Journal of Biomedical Nanotechnology
·
2017
·Vol. 13
·期 3
·243-254
·
IF 4.521
Jiang Dandan,Zhang Xiaopeng,Yu Dexin,Xiao Yanan,Wang Tianqi,Su Zhihui,Liu Yongjun,Zhang Na
Magnetic resonance imaging (MRI) contrast agents with tumor-microenvironment changeable relaxivity are effective to increase the sensitivity and selectivity of MRI in tumor diagnosis. In this study, pH-sensitive Gd-loaded Poly(L-lysine)/ Carboxymethyl Chitosan Nanoparticles (Gd-PCNPs)
were developed as relaxivity-changeable MRI contrast agents based on the "on–off" switchable strategy. The "on–off" switchable nano-contrast agents were capable of releasing Gd3+ in response to physical stimulation, with structure transformed. Gd-PCNPs could responsively
disassemble in an acidic tumor-microenvironment and increase the exchange of protons between water molecules and Gd3+ ions, thus selectively enhance the relaxivity in tumor area. Gd-PCNPs were self-assembled via electrostatic interaction between poly(L-lysine)-diethylenetriamine
pentaacetic acid-gadolinium and pH-sensitive carboxymethyl chitosan (CMCS). Gd-PCNPs exhibited spherical shape with uniform particle size distribution (166.00 ± 1 .71 nm) and negative zeta potential (–13.2 ± 4.7 mV). The relaxivity of Gd-PCNPs increased from 6.618 mM–1
· s–1 to 10.008 mM–1 · s–1 when the pH values decrease from 7.4 to 6.0, which was higher than Magnevist® (3.924 mM–1 · s–1 at both pH 7.4 and 6.0 (p <0 05). The
changeable relaxivity of Gd/PCNPs would result in enhanced tumor/normal tissue signal contrast, which was verified by in vivo MRI test. In vivo MRI test showed that the signal of Gd-PCNPs was significantly enhanced with prolonged imaging time in tumor tissue compared to Magnevist®
(p <0 05). Furthermore, Gd-PCNPs exhibited unobvious in vitro cytotoxicity under the experimental concentrations in B16 cells. No obvious damage was observed in the different tissues of mice. These results indicated that the relaxivity-changeable Gd-PCNPs exhibited demonstrated
sensitivity and selectivity in tumor diagnosis with a great potential as a novel MRI contrast agent.
研究领域:
医学影像
·
纳米技术
·
癌症研究
·
生物医学工程
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EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
·
2026
·
IF 5.1
Qiang Huang,Zhiren Zou,Dian Cheng,Ruyu Gao,Lin Zhou,Wencheng Wu,Yinyin Yuan,ChangJu Chun,Yuye Huang,Guang Liang,Wenqi Wu
Co-amorphous systems have emerged as a promising strategy to enhance the aqueous solubility of poorly water-soluble drugs. Although amino acids have been widely studied as co-formers, some amino acid-based co-amorphous systems exhibit limited dissolution improvement and inadequate physical stability. In this study, glycyl- l -histidyl- l -lysine (GHK) was selected as a co-former due to its biocompatibility and multiple functional groups, which were expected to enhance the dissolution and the physical stability of pure amorphous drugs. Basic mebendazole, neutral tadalafil and acidic indomethacin were selected as the model drugs. Glycine, histidine and lysine were used as co-formers for comparison. All drug-co-former combinations were prepared by ball milling at a 1:1 molar ratio. GHK achieved complete amorphization with all three model drugs within 15 min of milling, whereas most amino acid-based mixtures remained partially crystalline even after 60 min of milling. The formation of homogeneous amorphous forms was confirmed by differential scanning calorimetry, indicated by a single glass transition temperature in the drug-GHK system. The drug-GHK systems exhibited significantly enhanced dissolution performance, including faster dissolution rates, higher drug concentration and sustained supersaturation (up to 1440 min) compared to both the pure crystalline and amorphous drugs. Furthermore, physical stability studies confirmed that GHK-based systems maintained amorphous forms for at least 6 months, outperforming both amino acid-based counterparts and pure amorphous drugs. Notably, salt formation and hydrogen bonding were associated with the observed improvements in dissolution performance and physical stability. In conclusion, GHK represents a promising co-former, expanding the design space for co-amorphous systems.
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Polymers
·
2026
·Vol. 18
·期 3
·332
·
IF 4.9
Jinqiang Zhu,Wenjun Qin,Bo Wu,Haining Li,Cui Cheng,Xiao Han,Xiwen Jiang
Background: Chronic diabetic wounds, particularly diabetic foot ulcers (DFUs), are prone to recurrent infection and delayed healing, resulting in substantial morbidity, mortality, and economic burden. Multifunctional wound dressings that combine antibacterial, antioxidant, conductive, and self-healing properties may help to address the complex microenvironment of chronic diabetic wounds. Methods: In this study, ε-poly-L-lysine and amino-terminated polyethylene glycol were grafted onto carboxylated single-walled carbon nanotubes (SWCNTs) via amide coupling to obtain ε-PL-CNT-PEG. Aminated chondroitin sulfate (CS-ADH) and a catechol–metal coordination complex of protocatechualdehyde and Fe 3+ (PA@Fe) were then used to construct a dynamic covalently cross-linked hydrogel network through Schiff-base chemistry. The obtained hydrogels (Gel0–3, Gel4) were characterized for photothermal performance, rheological behavior, microstructure, swelling/degradation, adhesiveness, antioxidant capacity, electrical conductivity, cytocompatibility, hemocompatibility, and antibacterial activity in the presence and absence of near-infrared (NIR, 808 nm) irradiation. Results: ε-PL-CNT-PEG showed good aqueous dispersibility, NIR-induced photothermal conversion, and improved cytocompatibility after surface modification. Incorporation of ε-PL-CNT-PEG into the PA@Fe/CS-ADH network yielded conductive hydrogels with porous microstructures and storage modulus (G′) higher than loss modulus (G′′) over the tested frequency range, indicating stable gel-like behavior. The hydrogels exhibited self-healing under alternating strain and macroscopic rejoining after cutting. Swelling and degradation studies demonstrated pH-dependent degradation, with faster degradation in mildly acidic conditions (pH 5.0), mimicking infected chronic diabetic wounds. The hydrogels adhered to diverse substrates and tolerated joint movements. Gel4 showed notable DPPH• and H 2 O 2 scavenging (≈65% and ≈60%, respectively, within several hours). The electrical conductivity was 0.19 ± 0.0X mS/cm for Gel0–3 and 0.21 ± 0.0Y mS/cm for Gel4 (mean ± SD, n = 3), falling within the range reported for human skin. In vitro, NIH3T3 cells maintained >90% viability in the presence of hydrogel extracts, and hemolysis ratios remained below 5%. Hydrogels containing ε-PL-CNT-PEG displayed enhanced antibacterial effects against Escherichia coli and Staphylococcus aureus , and NIR irradiation further reduced bacterial survival, with some formulations achieving near-complete inhibition under low-power (0.2–0.3 W/cm 2 ) 808 nm irradiation. Conclusions: A dynamic, conductive hydrogel based on PA@Fe, CS-ADH, and ε-PL-CNT-PEG was successfully developed. The hydrogel combines photothermal antibacterial activity, antioxidant capacity, electrical conductivity, self-healing behavior, adhesiveness, cytocompatibility, and hemocompatibility. These properties suggest potential for application as a wound dressing for chronic diabetic wounds, including diabetic foot ulcers, although further in vivo studies are required to validate therapeutic efficacy.
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International Journal of Electrochemical Science
·
2023
·
IF 1.5
Yuting Wang,Ying Xiong,Hui Tian,Shi Wang,Qianying Zhang,Mingjing Zhang,Hong Ding,Ye Gu,Anlin Peng
A novel disposable sensor modified with MWNTs/Al 2 O 3 /poly-L-lysine film was developed for detection of clinical 17β-estradiol (17β-E 2 ) urine samples in this study. The electrochemical behavior of 17β-E 2 was greatly increased due to the good electrocatalytic activity of MWNTs/Al 2 O 3 /poly-L-lysine nanocomposites. The optimum experimental conditions such as scan rate, pH, accumulation time were obtained, and the peak potentials of 17β-E2 was explored at about 530mV. The peak current of 17β-E 2 measured by CV displayed the good linear property with the increasing of the concentrations. Under optimized conditions, the disposable sensor displayed excellent analytical performance with linear ranges of 17β-E 2 from 0.5nM to 50nM. And a very low detection limit of 0.014 nM was attained. Furthermore, the MWNTs/Al 2 O 3 /poly-L-lysine SPE was used to the rapid and successful determination of the hormone in spiked synthetic and real clinical pregnant woman urine samples with wonderful selectivity and sensitivity.
研究领域:
生物医学工程
·
临床检验医学
·
分子诊断学
·
电化学分析
·
纳米生物技术
·
激素检测
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INDUSTRIAL CROPS AND PRODUCTS
·
2023
·
IF 5.9
Shuai Zhang,Huangjingyi Chen,Zicong Shi,Ying Liu,Liang Liu,Juan Yu,Yimin Fan
A green and effective method was proposed for producing amino cellulose nanofibers (ε-PL-TOCNs) with a yield of > 60%, which was obtained via ε-poly- L -lysine-grafting of 2, 2, 6, 6-tetramethylpiperidine-N-oxyl (TEMPO)-oxidized cellulose (TOC). The different carboxyl group content of TOC, EDC/NHS molar ratio and their adding ways, ε-poly- L -lysine concentration on the yield, amino content and physicochemical properties of ε-PL-TOCNs were systematically investigated and optimized. The ε-PL-TOCNs with the characteristics of enhanced thermal stability, improved mechanical strength, and fluorescence properties was successfully produced. Moreover, the ε-PL-TOCNs was zwitterionic with both anionic and cationic charges due to the co-existence of carboxyl and amino groups, thus showed excellent dispersion stability in a wide pH range except for the isoelectric point around pH 9. Notably, the ε-PL-TOCNs possessed an excellent anti-microbial effect (>99%) against Escherichia coli and Saccharomyces cerevisiae at 200 μg/mL loading dosage because of the positive role of amino groups, which made it applicable to extend the freshness of fruits. This preparation approach of amino cellulose nanofibers was advantageous over traditional methods owing to green and efficient methods, which expanded the application of cellulose nanofibers in anti-microbial agent and food preservation.
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Food Packaging and Shelf Life
·
2022
·
IF 8.749
Xingzi Lan,Tingting Luo,Zhixin Zhong,Dongchao Huang,Chengfeng Liang,Yurong Liu,Han Wang,Yadong Tang
Gelatin nanofibrous membrane has attracted increasing attention as biodegradable and edible food packaging. However, the lack of bioactivity and the weak water resistance of gelatin nanofibrous membrane severely limits its application in food preservation. Therefore, in this study, antioxidant agent tea polyphenol (TP) and antibacterial agent ε-poly ( L -lysine) (ε-PL) were incorporated into gelatin nanofibrous membrane. To enhance the water resistance, the TP and ε-PL loaded gelatin nanofibrous membrane was cross-linked at high temperature. And the antibacterial activity and antioxidant activity of gelatin/TP/ε-PL nanofibrous membrane after cross-linking were investigated. The results showed that the gelatin/TP/ε-PL nanofibrous membrane exhibited homogenous bead-free structure. The UV–vis spectra confirmed the successful incorporation of TP and ε-PL into nanofibers . The water contact angle of the gelatin/TP/ε-PL nanofibrous membrane after cross-linking was increased. The fabricated nanofibrous membrane could prevent microbial penetration. And the nanofibrous membrane still exhibited excellent antibacterial activity and antioxidant activity after cross-linking. Finally, the MTT assay demonstrated the biocompatibility of nanofibrous membrane. The above results suggested that the prepared gelatin/TP/ε-PL nanofibrous membrane exhibited great potential in bioactive food packaging.
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MATERIALS & DESIGN
·
2022
·
IF 9.417
Fan Liu,Lei Xue,Li Xu,Jun Liu,Chungang Xie,Cen Chen,Yi Liu
The development of bioactive carrier with controlled delivery of Bone morphogenetic protein (BMP-2) is an important part in bone regeneration. Our study fabricated novel modified nanomaterials based on poly-l-lysine functionalized graphene oxide (GO-PLL) and explore its potential applications for BMP-2 delivery. GO-PLL was used to modify BMP-2 encapsulated bovine serum albumin (BSA) nanoparticles (GO-PLL/BMP-2) by self-assembling technology. The physiochemical and biological properties of GO-PLL modified BSA nanoparticles were investigated in vitro . Additionally, rabbit calvarial bone defects were created to investigate bone formation ability in the presence of GO-PLL/BMP-2 loaded alginate/gelatin scaffolds. The results suggested BSA particles were spatially interacted with GO-PLL complexes by strong electrostatic attraction. When compared with BMP-2 encapsulated BSA nanoparticles, a pronounced decrease in BMP-2 gentle release was observed in the presence of GO-PLL/BMP-2 nanoparticles, and extended to over 14-day release periods. More importantly, GO-PLL/BMP-2 nanoparticles significantly enhanced alkaline phosphatase activity (ALP) and matrix mineralization, and exhibited favorable bone formation ability when loaded into alginate/gelatin scaffolds. These results demonstrated that PLL functionalized GO complexes are considered as appropriate delivery system for BSA modification, and have great potential for BMP-2 delivery in bone regeneration.
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JOURNAL OF ETHNOPHARMACOLOGY
·
2023
·
IF 5.4
Gao-shang Chai,Juan Gong,Jia-jun Wu,Rui-kun Ma,Jun Zhu,Dong-dong Jia,Yu-qi Zhang,Xiao-run Zhai,Hong-xu Sun,Yun juan Nie,Peng Zhao,Yi-liang Xu,Hai tao Yu
Ethnopharmacological relevance Danggui Buxue decoction (DBD) is a classic herbal decoction consisting of Astragali Radix (AR) and Angelica Sinensis Radix (ASR) with a 5:1 wt ratio, which can supplement ‘blood’ and ‘qi’ (vital energy) for the treatment of clinical diseases. According to Traditional Chinese Medicine (TCM) theory, dementia is induced by Blood deficiency and Qi weakness, which causes a decline in cognition. However, the underlying mechanisms of DBD improving cognition deficits in neurodegenerative disease are no clear. Aim of the study This study aims at revealing the underlying mechanisms of DBD plays a protective role in the cognitive deficits and pathology process of Alzheimer's disease (AD). Materials and methods The APP/PS1 (Mo/HuAPP695swe/PS1-dE9) double transgenic mice were adopted as an experimental model of AD. Qualitative and quantitative analysis of 3 compounds in DBT was analyzed by HPLC. Morris water maze test, Golgi staining and electrophysiology assays were used to evaluate the effects of DBD on cognitive function and synaptic plasticity in APP/PS1 mice. Western blot , immunofluorescence and Thioflavin S staining were used for the pathological evaluation of AD. Monitoring the level of ATP, mitochondrial membrane potential, SOD and MDA to evaluate the mitochondrial function, and with the usage of qPCR and CHIP for the changes of histone post-translational modification. Results In the current study, we found that DBD could effectively attenuate memory impairments and enhance long-term potentiation (LTP) with concurrent increased expression of memory-associated proteins. DBD markedly decreased Aβ accumulation in APP/PS1 mice by decreasing the phosphorylation of APP at the Thr668 level but not APP, PS1 or BACE1 . Further studies demonstrated that DBD restored mitochondrial biogenesis deficits and mitochondrial dysfunction. Finally, the restored mitochondrial biogenesis and cognitive deficits are under HADC2-mediated histone H4 lysine 12 (H4K12) acetylation at the peroxisome proliferator-activated receptor-gamma coactivator 1α (PGC-1α) and N-methyl-D-aspartate receptor type 2B (GluN2B) promoters. Conclusions These findings reveal that DBD could ameliorate mitochondrial biogenesis and cognitive deficits by improving H4K12 acetylation. DBD might be a promising complementary drug candidate for AD treatment.
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BIORESOURCE TECHNOLOGY
·
2021
·
IF 9.642
Cheng Wang,Xi Chen,Yingying Jiang,Na Li,Ping Zhu,Hong Xu
Facile and green fabrication of reduced graphene oxide on loofah sponge (rGOLS) carrier was applied for cell immobilization of ε-Poly- l -lysine (ε-PL) production. Due to surface properties including large specific surface area, high polarity, and low interaction energy, rGOLS-1 was employed as the optimum rGOLS to enhance immobilization of Streptomyces albulus . Compared with raw LS, batch experiments showed rGOLS-1 facilitated superior cell vitality for ε-PL production due to the presence of reduced graphene oxide. In the sequential fed-batch cultivation of Streptomyces albulus using rGOLS-1 with an aerobic plant fibrous-bed bioreactor (rGOLS-1-AFPB), the maximum ε-PL concentration and productivity reached to 39.2 ± 0.63 g/L and 0.48 g/L/h. The cells immobilized in rGOLS-1 with high vitality and ε-PL production efficiency were reused six times over a period of 624 h. This research afforded an effective approach to enhance the fermentation performance of immobilized cells with the design of an advanced immobilization carrier.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Jiadong Pang,Baoxiong Zhuang,Li-Ming Zhang
Pancreatic cancer is one of the most lethal malignancies in the world and remains one of the leading causes of cancer related death. For its treatment, a lot of investigations have dealt not only with individual chemotherapy by using polymeric carriers to deliver anticancer drugs, but also with individual gene therapy by using polymeric carriers to deliver nucleic acids such as small interfering RNA (siRNA) and plasmid DNA . However, relatively few studies have been focused on the co -delivery of gene and anticancer drug by multifunctional polymeric carriers for its synergistic therapy. In this work, a DPLL-functionalized amylose (ADP) was prepared by the click reaction between azidized amylose and propargyl focal point poly( l -lysine) dendrons, and then used to co-deliver plasmid pIRES2-EGFP-TNFα and curcumin for pancreatic cancer treatment. Due to the internal hydrophobic cavity of amylose component, ADP could load efficiently curcumin with anticancer activity and showed a sustained release behavior. Moreover, the curcumin-loaded ADP could form colloidally stable nanocomplexes with plasmid DNA in aqueous system due to the existence of cationic poly( l -lysine) dendrons and exhibited high gene transfection efficiency. The in vitro and in vivo tests confirmed the effectiveness of using ADP to co-deliver plasmid pIRES2-EGFP-TNFα and curcumin for synergistic therapy of pancreatic cancer.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Jiajie Ye,Anni Li,Tianshu Chu,Xin Pan,Bingfang He
Penicillin G acylase (PGA) from Providencia rettgeri PX04 (PrPGA) was utilized to synthesize β-lactam antibiotics. Poly-lysine supported cross-linked enzyme aggregates (PL-CLEAs) were prepared using PGA. Addition of poly-lysine significantly increased retention of PGA activity in CLEAs, with a decrease in the synthesis/hydrolysis (S/H) ratio. PL-CLEAs with 0.56 mg/mL poly-lysine retained 83% of free PGA activity, and displayed a higher S/H ratio than that of the free enzyme. Both PL-CLEAs and CLEAs exhibited high pH and thermal stabilities . PL-CLEAs possessed the best stability profile, and the lowest α value [( k cat / K m ) Ps /( k cat / K m ) AD ], and was most effective at amoxicillin synthesis. A >94% yield of amoxicillin was achieved using a D-HPGME/6-APA ratio of 1.2:1 (240 mM, 200 mM), with fed-batch addition of D-HPGME. PL-CLEAs displayed excellent operational stability during amoxicillin synthesis. Over 97% of initial conversion was retained after twenty rounds of catalysis. PL-CLEAs exhibited greater potency than CLEAs in practical catalysis, permitting a higher concentration of reactants.
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FOOD CHEMISTRY
·
2018
·
IF 4.946
Yuting Li,Lin Li,Marianne N. Lund,Bing Li,Yi Hu,Xia Zhang
(−)-Epicatechin (EC) and (−)-epigallocatechin gallate (EGCG) (0.001–5%) were found to reduce N ε -(carboxymethyl) lysine (CML) concentrations by up to 0.80 ± 0.11 mM and 0.85 ± 0.07 mM, respectively, as quantified by HPLC-MS during heating at 80 °C and 100 °C in a glucose-lysine model system at pH 5, 7 and 8, which mimic different food processing conditions. With the addition of 1 mM EC, the free CML concentration in coconut milk was reduced to 5.7 ± 0.1 μg/mL (∼2.8 × 10 −2 mM), which was significantly lower than that in unheated coconut milk (6.1 ± 0.2 μg/mL (∼3.0 × 10 −2 mM), p < 0.05) indicating that the free CML may be eliminated by the addition of EC in coconut milk. Products of EC/EGCG quinones reacted with CML by a Michael addition or Schiff base addition in model systems were tentatively identified by UPLC-ESI-MS/MS.
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