INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2024
·Vol. 59
·期 6
·3823-3833
·
IF 2.6
Yufeng Lin,Weiqian Luo,Beibei Xiong,Jian Guo,Jinmei Wang,Wenhao Tan,Xiaoquan Yang
Summary In this work, soy protein isolates (SPIs) were subjected to microparticulation, turning soy protein ingredients into microparticles with improved thermal stability which could be used in high-protein foods. The pH is a critical determinant in microparticulation. Our work is mainly to investigate the effect of pH on the formation and properties of protein microparticles. To clarify the effects of pH conditions on the properties of the resulting particles, microparticulation was performed at different pH conditions (7.0, 6.0 and 5.0). Aggregation behaviours described by the parameters calculated from the protein dispersion viscosity combined with the observation of the morphology change were used to analyse the particle formation process. For the microparticles prepared at pH 5.0, soy proteins were more inclined to aggregate into clusters on a micro-scale which had a flexible conformation. After spray drying, This powder could be easily dispersed in water, creating a dispersion with a protein concentration of 10 wt%. At this concentration, the thermal gelation capacity of the proteins was observed to decrease. Conversely, the tendency of protein aggregation was largely restricted when soy protein microparticulation was carried out at pH 7.0. As these soy protein particles in nano-scale were dispersed in water, a decrease rather than an increase was recorded in the viscosity of this dispersion after it was heated, suggesting that these particles had improved thermal stability. This work has demonstrated that implementing appropriate pH conditions during microparticulation can prepare soy protein particles with different sizes and rheological properties, which might meet the demands of various food processing.
研究领域:
食品科学
·
蛋白质化学
✓ 已验证
DOI
高影响因子
JOURNAL OF BIOMATERIALS APPLICATIONS
·
2017
·
IF 2.31
Mazhar Ali Raja,Muhammad Arif,Chao Feng,Shah Zeenat,Chen-Guang Liu
A novel pH-responsive polymer based on amphiphilic N-acetyl histidine and arginine-grafted chitosan was synthesized using N-acetyl histidine as hydrophobic segment and arginine as hydrophilic segment by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide-mediated coupling reactions as anticancer drug delivery system for doxorubicin. The structure of the synthesized polymer was confirmed by Fourier transform infrared and 1H nuclear magnetic resonance analysis. Due to self-association behavior, N-acetyl histidine and arginine-grafted chitosan structured nanoparticles with in size range of 204 nm. N-acetyl histidine and arginine-grafted chitosan with different substitution degree of N-acetyl histidine were initially prepared and characterized. The critical micelle concentration decreased with increasing substitution degree of N-acetyl histidine. Furthermore, N-acetyl histidine and arginine-grafted chitosan nanoparticles exhibited an acidic pH-triggered aggregation and disassembling nature. The doxorubicin-encapsulated nanoparticles based on synthesized conjugate (N-acetyl histidine and arginine-grafted chitosan/doxorubicin nanoparticles) showed a sustained drug release pattern, which could be hastened under acidic pH conditions but delayed with increasing substitution degree of N-acetyl histidine. Anticancer effects demonstrated that N-acetyl histidine and arginine-grafted chitosan/doxorubicin nanoparticles could suppress both sensitive and resistant human breast tumor cell line (MCF-7) efficiently in a dose- and time-dependent pattern. Confocal microscopy results evidenced increased cellular uptake and enhanced retention of the synthesized nanoparticles in drug-resistant cells demonstrating better efficacy of nanoparticles over native doxorubicin. These results suggest that N-acetyl histidine and arginine-grafted chitosan/doxorubicin nanoparticles might be promising carriers for delivery of hydrophobic drug doxorubicin against drug-resistant tumors.
研究领域:
药学
·
生物材料
·
肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2019
·
IF 4.959
Chengqiang Ding,Hongwei Wu,Zheng-Zhi Yin,Jun Gao,Datong Wu,Yong Qin,Yong Kong
Hydrophilic poly (acrylic acid) (PAA) and hydrophobic α-tocopherol succinate (TOS) were integrated via a two-step amidation with cystamine (Cys) as the linkage, and then the self-assembly of amphiphilic PAA-cys-TOS occurred in the aqueous solution of methotrexate (MTX), an anti-cancer drug, resulting a vesicle structured drug carrier. Since the disulfide (–S–S–) bridge of Cys is sensitive to glutathione (GSH) and the amide bonds in PAA-cys-TOS are sensitive to pH, disulfide-cleavage- and pH-triggered drug delivery was achieved with the amphiphilic self-assembly. Of particular interest was that the topography of the self-assembly varied remarkably during the triggered delivery, which was indicated by TEM results.
研究领域:
药剂学
·
生物化学
·
肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2017
·
IF 3.887
Tingsheng Yan,Jinju Cheng,Zongjun Liu,Feng Cheng,Xinjing Wei,Jinmei He
In order to optimize the chemotherapeutic efficacy of doxorubicin (DOX) and improve the photodynamic therapeutic effectiveness of rose bengal (RB), a mesoporous silica nanoparticle system was designed as the carrier of RB and DOX for chemo-photodynamic combination therapy. A pH-sensitive strategy has been exploited to enhance the delivery efficiency. Our results suggested that the production of singlet oxygen was independent of the release of RB while strongly influenced by the external DOX layer. This method showed several benefits, including accelerating cellular uptake of the payloads and enabling chemo-photodynamic combination therapy for synergistic cancer treatment. Our study provides a new way for co-delivery of chemotherapy agents and photosensitizers.
研究领域:
生物医学工程
·
药剂学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Small
·
2025
·
IF 12.1
Bingzhao Wu,Lianning Li,Hu Huang,Zhiyu Feng,Xinlong Zhang,Lei Zhang,Chunyu Zhao
Bottom-up construction of artificial cells is pivotal for investigating the functional and structural evolution of protocell systems. Here, a streamlined methodology is reported for constructing a protocell model that exhibits pH-adaptive responsiveness and functions as an efficient microreactor for cascade biochemical reactions. These protocells are engineered through liquid–liquid phase separation (LLPS) of poly(diallyldimethylammonium chloride) (PDDA) and adenosine triphosphate (ATP), yielding coacervate microdroplets that serve as nucleation sites for spontaneous self-assembly of phospholipid semipermeable membranes. By tuning the pH environment of the membranized protocells, the hydrolytic property of ATP under acidic conditions modulates the surface charge density of coacervates, triggering elevated surface potential and altered interfacial tension, which ultimately induce secondary phase separation inside the protocells to yield dynamically reconfigurable microcompartments. Notably, introduction of small-molecule substrates triggers enzymatic reactions within the molecularly crowded lumen of the protocells, which in turn drives pH-mediated micro-compartmentalization, enabling the coupling of biocatalysis with structural dynamics. This protocell model, characterized by programmable dynamic micro-compartmentalization, offers distinct advantages for applications in cellular mimicry, synthetic protobiology, and the development of artificial biomolecular microreactors.
研究领域:
合成生物学
·
生物物理学
·
分子自组装
·
原始细胞模拟
·
酶催化级联反应
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2020
·Vol. 10
·期 61
·36971-36979
·
IF 3.119
Hua Tian,Yongcheng Dai,Wenzhe Fu,Haifang Liu,Mengting Li,Meiyuan Lv,Xueqiong Yin
In this work, a multifunctional ratiometric fluorescence (FL) nanohybrid (CSCDs@DC) was synthesized from chitosan based carbon dots (CSCDs) and dansyl chloride (DC) at room temperature. The CSCDs@DC revealed strong FL intensity, great stability and excellent anti-photobleaching properties. Herein, CSCDs@DC was responsive to pH value in the range of 1.5–4.0 and exhibited color-switchable FL properties between acidic and alkaline environments. In addition, CSCDs@DC showed good selectivity and sensitivity towards Fe3+ ions. A good linear relationship for the Fe3+ ion detection was obtained in the range from 0 μM to 100 μM, with a detection limit of 1.23 μM. What's more, CSCDs@DC can be used as a fluorescent ink. It expressed superior optical properties after 3 months of storage or continuous exposure to UV light for 24 h. This study suggested that CSCDs@DC had potential in the detection of pH and metal ions, as well as showing promising application in the anti-counterfeiting field.
研究领域:
材料科学
·
分析化学
·
生物医学工程
✓ 已验证
DOI
高影响因子
BIOMATERIALS
·
2011
·
IF 7.883
Yunlu Dai,Cuimiao Zhang,Ziyong Cheng,Ping’an Ma,Chunxia Li,Xiaojiao Kang,Dongmei Yang,Jun Lin
In this study, we design a controlled release system based on CaF 2 :Ce 3+ /Tb 3+ -poly(acrylic acid) (PAA) composite microspheres , which were fabricated by filling the pH-responsive PAA inside CaF 2 :Ce 3+ /Tb 3+ hollow spheres via photopolymerization route. The CaF 2 :Ce 3+ /Tb 3+ hollow spheres prepared by hydrothermal route possess mesoporous structure and show strong green fluorescence from Tb 3+ under UV excitation. Doxorubicin hydrochloride (DOX), a widely used anti-cancer drug, was used as a model drug to evaluate the loading and controlled release behaviors of the composite microspheres due to the good biocompatibility of the samples using MTT assay . The composite carriers provide a strongly pH-dependent drug release behavior owing to the intrinsic property of PAA and its interactions with DOX. The endocytosis process of drug-loaded microspheres was observed using confocal laser scanning microscopy (CLSM) and the in vitro cytotoxic effect against SKOV3 ovarian cancer cells of the DOX-loaded carriers was investigated. In addition, the extent of drug release could be monitored by the altering of photoluminescence (PL) intensity of CaF 2 :Ce 3+ /Tb 3+ . Considering the good biocompatibility, high drug loading content and pH-dependent drug release of the materials, these hybrid luminescent microspheres have potential applications in drug controlled release and disease therapy.
研究领域:
生物材料学
·
药理学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Junjie Fan,Jianpeng Cui,Tianhao Zhang,Jianwen Hu,Ziqi Wu,Mingshuo Chi,Wenqing Xie,Shuangqing Sun,Songqing Hu
In this study, we successfully synthesized silica coated Fe 3 O 4 nanoparticles (Fe 3 O 4 @SiO 2 NPs) and further animated their surface to create amino modified Fe 3 O 4 @SiO 2 (Fe 3 O 4 @SiO 2 -NH 2 ) NPs. By taking advantage of the pH-responsive dynamic (covalent) imine bond between the hydrophilic Fe 3 O 4 @SiO 2 -NH 2 NPs and the hydrophobic cinnamaldehyde molecule (R), we developed dynamic covalent Fe 3 O 4 @SiO 2 (Fe 3 O 4 @SiO 2 -R) NPs with switching wettability . These NPs were then utilized to form Pickering emulsions , and whose stability and pH/magnetic response were thoroughly investigated. As the NPs concentration in the emulsion increased, the emulsion stability initially improved but then subsequently slightly decreased. Emulsifiers exhibited emulsifying effect on various oil phases, with paraffin oil demonstrating the best effect. By adding Hydrochloric acid to the emulsion, we successfully broke the imine bond at a pH value of 2.8, resulting in demulsification. Applying a magnetic field also achieved demulsification. After five cycles of pH or magnetic response-induced emulsification and demulsification, a stable Pickering emulsion could still be obtained. Stimuli-responsive Pickering emulsions have been extensively studied, but there is limited research on double-responsive Pickering emulsions. This study presents a novel design of Pickering emulsions with pH/magnetic dual responsiveness, offering great potential for oil-water separation applications.
研究领域:
材料科学
·
化学工程
·
界面科学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Water Process Engineering
·
2024
·
IF 6.3
Guoqi Liu,Jihong Fu,Junli Yu,Yaxue Zhang,Ronglan Wu
pH-responsive smart materials with switchable wettability have a great potential application in bidirectional oil-water separation. In this work, a simple, low-cost and environmentally friendly strategy is developed to construct a pH-responsive melamine sponge (MS) with reversible wettability via polydopamine (PDA) modification followed by grafting long-chain alkanes with carboxyl (–COOH) and methyl (–CH 3 ) groups. The as-modified sponge exhibits unique pH-responsiveness, that is, the water contact angle (WCA) is measured to be 144.9° under acidic and neutral environments, while the wettability is radically changed from hydrophobic to hydrophilic under alkaline conditions. Moreover, in alkaline environment, the pH-responsive sponge can rapidly release the absorbed oil, demonstrating a controllable desorption performance. Also, the prepared sponge is utilized to accomplish controllable separation of various oil/water mixtures, as well as to effectively separate oil-in-water emulsions. Furthermore, after 50 cycles, the oil-water separation efficiency was still higher than 92 %. This smart wettability sponge has an extensive application prospect in controlled oil-water separation and on-demand oil-spill remediation.
研究领域:
材料科学
·
环境工程
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2024
·Vol. 48
·期 21
·9549-9558
·
IF 2.7
Ting-Ting Li,Mengdan Jia,Shuxia Li,Ying Zhang,Xiaomeng Wang,Sheng Chu,Bing-Chiuan Shiu,Ching-Wen Lou,Jia-Horng Lin
Oil spills and industrial wastewater discharge have inflicted severe damage upon the ecological environment and human health. Therefore, the development of an environmentally friendly and pollution-free membrane material for oil–water separation is imperative. This study presents the development of a novel oil–water separation material (PP–PPy–pHR) that is environmentally benign and capable of switching between wetting properties. The material was synthesised by dopamine modification, treatment with a pH-responsive polymer and the use of a high-efficiency photothermal layer composed of self-assembled polypyrrole (PPy), and it not only separates incompatible oil–water mixtures but also efficiently separates oil–water emulsions under different pH conditions. Due to the good photothermal effect of PPy, the evaporation rate of PP–PPy–pHR composite membrane loaded with PPy was 3.95 times higher than that of pure water for oily wastewater. In addition, the PPy–pHR composite membrane exhibited excellent durability and high stability in extremely acidic and alkaline solutions, showing remarkable potential for oil–water separation applications.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
DYES AND PIGMENTS
·
2024
·
IF 4.1
Xingyun Gao,Jiaguang Meng,Yongzhen Wang,Yaming Liu,Chao Zhi,Lu Yang,YuQing Chen,Chao Wang
Relative humidity (RH), temperature, and pH as important environmental parameters that are closely related to industry, agriculture, and people's life. Therefore, the development of a colorimetric sensor that can monitor the RH, temperature, and pH in real-time is highly desired. In this work, a fabric-based flexible colorimetric sensor is easily manufactured by screen printing the mixture of cresol red (CR), thymol blue (TB) and boric acid (H 3 BO 3 ) on the fabric. Compared with the traditional single stimulus colorimetric sensor, this fabric device can respond to multiple stimulus factors including humidity, temperature, or pH. The simple coating treatment can endow the colorimetric sensor with fast response without compromising its flexibility. Exposed to different stimulus conditions, the colorimetric sensor has a unique color change effect, such as humidity-responsive pink-yellow, temperature-responsive yellow-pink, and pH-responsive pink-purple. The flexibility and versatility of the fabric sensors are showcased with the applications in temperature/humidity/pH detection, cultural relics protection and information encryption. We believe the colorimetric sensor would have potential in environmental monitoring, health care, encrypted information patterns, and intelligent wearables, etc.
研究领域:
材料科学
·
环境科学
·
传感技术
✓ 已验证
DOI
高影响因子
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2020
·
IF 2.69
Meijie Wang,Kaiwei He,Jilu Li,Tong Shen,Yang Li,Yiting Xu,Conghui Yuan,Lizong Dai
In this article, we introduce a pH-responsive charge-reversible and photo-crosslinkable polymer nanoparticle. It is prepared via typical self-assembly from a block copolymer poly((7-(4-vinyl-benzyloxyl)-4-methylcoumarin)-co-acrylicacid)-b-poly((2-dimethylamino) ethyl methacrylate)-co-styrene) (P(VBMC-co-AA)-b-P(DMAEMA-co-St)), whose two blocks have different ionizable moieties. In an aqueous solution of pH ≤ 4, the cationic polymer nanoparticles are formed due to the fully protonated PDMAEMA. At a pH ranging from 5.0 to 7.8, partially ionized PAA and protonated PDMAEMA lead to the formation of polymer nanoparticles with a mixed shell. In a pH range of 8–10, a large amount of precipitation is produced within the isoelectric point (IEP) region because of the weak hydrophilic two blocks. In an aqueous solution of pH ≥ 10, polymer nanoparticles are reformed with PAA shell and P(DMAEMA-co-St) core. The coumarin groups of polymer can undergo photo-crosslinking and photo-cleavage reactions under UV light irradiation at λ = 365 nm and λ = 254 nm, respectively. The reversible nature of the photo-reaction can regulate the reversal of polymer nanoparticles. Furthermore, the aggregation-induced fluorescence emission (AIFE) property of polymer nanoparticles at different pH is tested by fluorescence emission spectra. The results indicate that the aggregation state of coumarin blocks in solution also changes with the pH value. The DOX release experiment in vitro shows that the release behavior of DOX-loaded nanoparticles can be adjusted by pH and light to achieve significant control. The inhibitory effect on the growth of tumor cells is displayed by cellular uptake and cytotoxicity test in vitro. The self-assembly system of polymer nanoparticles can be cooperatively controlled by multiple stimulations and displays potential applications in controlled drug delivery.
研究领域:
材料科学
·
药学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）