JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·Vol. 23
·期 1
·1-22
·
IF 12.6
Yang Chuan,Xie Langlang,Deng Zihan,Ai Hongbo,Xiang Tingwen,Yan Xiaojing,Ling Zhiguo,Xiao Shiyu,Tang Yong,Huang Gang,Luo Fei,Chen Yueqi
Dysbiosis of gut microbiota significantly exacerbates the progression of rheumatoid arthritis (RA). Targeting gut microbiota may present a promising therapeutic strategy for RA. Gold nanospheres (GNS), known for excellent biocompatibility, stability and minimal toxicity, have emerged as precise modulators of gut microbiota, reshaping intestinal environments to treat various inflammatory diseases. Our study found that oral administration of 60-nm GNS effectively ameliorated collagen-induced arthritis (CIA) in mice, with a marked reduction in disease severity and synovial inflammation. Specifically, GNS notably enriched the probiotic genus Ligilactobacillus while restoring intestinal barrier function by upregulating tight junction proteins Claudin-1 and ZO-1. Targeted metabolomics analysis revealed GNS substantially increased the production of indole-3-propionic acid (IPA) and indole-3-acetic acid (IAA) in gut, which were shown to activate the aryl hydrocarbon receptor (AhR) pathway. Mechanistic studies demonstrated that the IPA/IAA mixture inhibited PTEN ubiquitination, stabilizing PTEN protein levels and suppressing NF-κB activation in synovial tissues. These changes correlated with reduced synovial hyperplasia and inflammatory infiltration. Our findings established GNS as an effective nanomodulator of the gut-joint axis, providing novel insights into microbiota-targeted therapies for RA and other inflammatory diseases. Graphical abstract
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BMC Complementary Medicine and Therapies
·
2025
·Vol. 26
·期 1
·9
·
IF 3.4
Zhu Liangting,Hu Junjie,Li Juan,Qu Xinyue,Zhou Xian,Gan Guoping,Liu Yanwen,Liu Songlin,Chen Xin
Background Sini San (SNS) is a traditional Chinese medicinal formulation originating from the “ Treatise on Febrile Diseases ”, an ancient text that has been historically employed for treating depression. However, the underlying mechanisms by which SNS potentially ameliorates symptoms of depression remain unclear. This study aimed to elucidate the role of gut microbiota in tryptophan metabolism and examine the involvement of short-chain fatty acids (SCFAs) in the putative antidepressant effects of SNS. Methods A rat model of depression using chronic unpredictable mild stress (CUMS) was established through simultaneous modeling and treatment for six consecutive weeks. The possible therapeutic effect of SNS on CUMS-induced rats was thoroughly assessed using body weight measurements, open-field tests, and sucrose preference tests. Alterations in rat gut microbiota were analyzed using full-length 16 S rRNA third-generation sequencing. Furthermore, metabolomic analysis was performed on fecal samples. An enzyme-linked immunosorbent assay (ELISA) was used to quantify tryptophan (Trp), serotonin (5-HT), and kynurenine (Kyn) in the hippocampal and colonic tissues. Reverse transcription quantitative PCR (RT-qPCR) was used to measure the mRNA levels of TPH-1 and TPH-2 in hippocampal and colonic tissues. Immunofluorescence was used to assess hippocampal IDO-1 and IBA-1 expression. Finally, gas chromatography-mass spectrometry (GC-MS) was used to analyze the SCFAs content in fecal samples. Results Administration of SNS significantly alleviated depressive symptoms induced by CUMS, as demonstrated by increased body weight, improved sucrose preference, and a statistically significant decrease in immobility time during the forced swim test ( P < 0.05). The Shannon and Simpson indices were elevated following SNS treatment, signifying an enhancement in both the quantity and variety of the gut microbiota. Analysis using 16 S rRNA gene sequencing revealed that SNS influenced the composition of the gut microbiome, favorably increasing the presence of beneficial bacteria, such as Firmicutes , Lactobacillaceae , Ruminococcaceae , and Oscillospiraceae , while simultaneously decreasing the levels of potentially detrimental bacteria, such as Muribaculaceae , Prevotellaceae , Lachnospiraceae , and Alloprevotella . Fecal metabolomic studies identified 87 metabolites, of which 57 were differentially expressed, most notably l-tryptophan and phenylalanine. These metabolites were primarily linked to 15 metabolic pathways, with notable enrichment in the biosynthetic pathways of phenylalanine, tyrosine, and tryptophan, as well as the metabolic pathways of arachidonic acid and phenylalanine. Metabolomic profiling indicated that SNS stimulated the kynurenine pathway, leading to increased levels of metabolites with antidepressant properties, such as Trp, 5-HT, and Kyn, in both hippocampal and colonic tissues. In addition, SNS promoted the synthesis of SCFAs, particularly butyrate and propionate, which are known for their neuroprotective properties. Conclusions The present study suggests that the antidepressant effects of SNS can be attributed to its ability to modulate the structure of the gut microbiota, consequently regulating tryptophan metabolism and SCFA levels, which ultimately ameliorate symptoms of depression. These findings provide experimental evidence supporting the concept of the brain-gut axis in depression treatment and lay a foundation for further investigation of the underlying antidepressant mechanisms of the SNS.
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Nanoscience and Nanotechnology Letters
·
2017
·Vol. 9
·期 11
·1700-1707
·
IF 1.889
Zou Jiao,Huang Lele,Jiang Xinyu,Jiao Feipeng,Yu Jingang
A novel chiral electrochemical sensor was fabricated and used for recognition of tryptophan (Trp) enantiomers. Graphene oxide (GO) was prepared by the modified Hummers' method. Moreover, the suspensions/solutions of GO, bovine serum albumin (BSA) and chitosan (CS) in deionized-distilled
water were coated onto the surface of GCE by a layer-by-layer drop method, and the novel triple layered GO/BSA/CS composite modified glassy carbon electrode (GO/BSA/CS/GCE) was fabricated. The electrochemical recognition and quantification of the Trp enantiomers on the GO/BSA/CS/GCE
were performed using differential pulse voltammetry (DPV). The Trp monomer concentrations exhibited a good linear relationship with the peak current values. The GO/BSA/CS/GCE exhibited good coefficients of determination (R
2
D-Trp
= 0.98917 and R
2
L-Trp
= 0.94824) for D- and L-Trp enantiomers in the concentration range of 1 to 8 mM, respectively. The GO/BSA/CS/GCE fabricated in this study exhibited superior recognition performance, good oxidation potential and current response signals for D- and L-Trp. The novel GO/BSA/CS/GCE
fabricated in this study exhibited potential to be used as a convenient, fast and efficient chiral electrochemical sensor for qualitative and quantitative determination of Trp enantiomers.
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FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Huijun Li,Xinyao Luo,Fengxia Li,Xiaochuan Ye,Meng Yu,Zhongmei Zou
Citri Reticulatae Pericarpium (CRP) is a traditional Chinese medicinal herb with both medicinal and edible properties. The supercritical CO 2 extract of Citri Reticulatae Pericarpium (C-CRP) has shown potential antidepressant effects, but the precise mechanisms remain unclear. In this paper, we confirmed that C-CRP alleviated depressive-like behaviors in a chronic unpredictable mild stress (CUMS) depression rat model, as evidenced by increased sucrose preference in the sucrose preference test (SPT), increased total distance traveled in the open field test (OFT), reduced immobility time in both the tail suspension test (TST) and forced swim test (FST), and elevated serum levels of 5-hydroxytryptamine (5-HT), dopamine (DA), and norepinephrine (NE) in CUMS rats. C-CRP reshaped gut microbiota dysbiosis, significantly regulating the abundance of specific bacteria, including Lactobacillus , unclassified_f_Lachnospiraceae , Blautia , Allobaculum , Prevotella , and UCG-005 via 16S rRNA sequencing. Untargeted/targeted metabolomics indicated that C-CRP normalized the dysregulated metabolic profiles in plasma, urine, and feces of depressed rats, and identified tryptophan metabolism and bile acid metabolism as key routes through which C-CRP exerts its effects, which was corroborated by pathway-specific protein and metabolite changes. Through multivariate correlation analysis, Blautia was identified as a crucial gut bacterium associated with the antidepressant efficacy of C-CRP, which was further confirmed in lipopolysaccharide (LPS)-induced depression-like mice by oral administration of Blautia obeum . Thus, the findings demonstrate that C-CRP exerts antidepressant effects by modulating specific gut bacteria—most notably Blautia obeum —and regulating tryptophan and bile acid metabolic pathways, thereby alleviating depression-like behaviors in the CUMS rats.
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CHIRALITY
·
2015
·Vol. 27
·期 9
·650-657
·
IF 1.886
Zhi Chen,Wei Zhang,Liping Wang,Huajun Fan,Qiang Wan,Xuehao Wu,Xunyou Tang,James Z. Tang
A novel method for chiral separation of flurbiprofen enantiomers was developed using aqueous two-phase extraction (ATPE) coupled with biphasic recognition chiral extraction (BRCE). An aqueous two-phase system (ATPS) was used as an extracting solvent which was composed of ethanol (35.0% w/w) and ammonium sulfate (18.0% w/w). The chiral selectors in ATPS for BRCE consideration were L-dioctyl tartrate and L-tryptophan, which were screened from amino acids, β-cyclodextrin derivatives, and L-tartrate esters. Factors such as the amounts of L-dioctyl tartrate and L-tryptophan, pH, flurbiprofen concentration, and the operation temperature were investigated in terms of chiral separation of flurbiprofen enantiomers. The optimum conditions were as follows: L-dioctyl tartrate, 80 mg; L-tryptophan, 40 mg; pH, 4.0; flurbiprofen concentration, 0.10 mmol/L; and temperature, 25 °C. The maximum separation factor α for flurbiprofen enantiomers could reach 2.34. The mechanism of chiral separation of flurbiprofen enantiomers is discussed and studied. The results showed that synergistic extraction has been established by L-dioctyl tartrate and L-tryptophan, which enantioselectively recognized R- and S-enantiomers in top and bottom phases, respectively. Compared to conventional liquid–liquid extraction, ATPE coupled with BRCE possessed higher separation efficiency and enantioselectivity without the use of any other organic solvents. The proposed method is a potential and powerful alternative to conventional extraction for separation of various enantiomers. Chirality 27:650–657, 2015 . © 2015 Wiley Periodicals, Inc.
研究领域:
手性化学
·
分离科学
·
分析化学
·
制药技术
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.5
Lu Wang,Chunyan Lu,Shugang Li,Tong Wu,Xiaomeng Ren,Shuang Song,Chunqing Ai
Aging is a multifactorial biological process in which chronic inflammation and oxidative stress are central to the development of age-related disorders, including neurodegenerative decline. Fucoidan, a sulfated polysaccharide extracted from brown algae, has well-documented anti-inflammatory and antioxidant effects, and therefore has the potential to be a neuroprotective agent against cognitive impairment associated with aging. In the present study, the major fucoidan fraction (LJF-2) isolated from Laminaria japonica was examined for its neuroprotective properties in a D-galactose induced aging mouse model. Oral administration of LJF-2 for 8 weeks significantly improved spatial learning and memory and suppressed neuroinflammatory responses and oxidative stress while significantly reducing the activation of astrocytes and microglia. These neuroprotective effects were linked to the regulation of key proteins involved in neuronal protection and synaptic function, such as neprilysin and synapsin, by cAMP response element-binding protein signaling. Furthermore, LJF-2 significantly remodeled the gut microbiota through a reduction in the abundance of the Bacteroidota, Proteobacteria, and several putative pathogenic genera, which enhanced the intestinal barrier integrity and modified the microbial metabolite profiles, especially those associated with tryptophan metabolism. Fecal microbiota transplantation experiments further confirmed the role of the gut microbiota modulated by LJF-2 in mediating its neuroprotective effects through reduction of oxidative stress and inflammation. Collectively, these findings suggest that LJF-2 may be a promising therapeutic approach to address the aging-related cognitive decline by modulating the gut–brain axis.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2022
·
IF 3.571
Mengmeng Song,Xianjie Sheng,Genyi Ye,Zhuicheng Xu,Xinru Li,Tong Xie,Haishan Deng,An Kang
Gut microbiota-derived tryptophan (TRP) metabolites, especially the indole derivatives, function as critical modulators of intestinal immune function and integrity via modulating the aryl hydrocarbon receptor pathway. Selective enrichment of these indole metabolites in biological samples is important for quantitative determination by routine methods such as HPLC. Here, we report a molecularly imprinted polymer (MIP) for solid-phase extraction (SPE) of TRP-derived indole metabolites from faeces of mice. The MIP was synthesized by surface polymerization by using indole-3-acetic acid (IAA) and acrylamide after cross-linking by ethylene glycol dimethacrylate (EGDMA) and initialed by 2′,2-azobisisobutyronitrile (AIBN). The MIPs were then characterized by transmission electron microscope (TEM) and fourier transform infrared spectroscopy (FTIR), and the adsorption capacity, selectivity and reusability of MIPs were systematically evaluated. Results showed that IAA-MIPs showed a uniformly distributed nanoshell layer with a thin shell thickness of 26 nm . The IAA-MIPs could selectively adsorb IAA, indole-3-propionic acid (IPA), and indole-3-lactic acid (ILA) in a mixed solution that also contains TRP and tyrosine. The adsorption capacity of IAA-MIPs only slightly decreased with the increase of recycling use. As purification material for SPE, the IAA-imprinted polymers (IAA-MIPs) were successfully applied to extract IAA, IPA, and ILA from normal and colitis mice for HPLC determination. Collectively, these surface molecularly imprinted polymers could find extensive use to selective enrichment of microbiota-derived indole metabolites in biological samples.
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JOURNAL OF SOLID STATE CHEMISTRY
·
2022
·
IF 3.656
Yue Chen,Shaobin Li,Li Zhang,Tao Jing,Jianxin Wang,Lijie Zhao,Fengbo Li,Chao Li,Jingyu Sun
Rational design and constructed new electrode material as high performance electrochemical sensor with outstanding selectivity, reproducibility and stability is an urgent demand for trace detection. Herein, a novel Ce-MOF/Ti 3 C 2 T X MXene composite as electrochemical sensor for L-Tryptophan (L-Trp) was constructed by utilizing the three-dimensional straw-sheaf-like Ce-MOF and Ti 3 C 2 T X MXene nanosheets . The combination of Ce-MOF and Ti 3 C 2 T X MXene not only significantly improves the conductivity of metal-organic framework (MOF) but also effectively prevents the stacking of Ti 3 C 2 T X MXene nanosheets. The electrochemical properties of the proposed sensor were investigated by electrochemical techniques in detail. The L-Trp sensor exhibits a wide linear range (0.2 μM–139 μM) and a low limit of detection (0.19 μM, S/N = 3). Moreover, the proposed sensor exhibits excellent selectivity, good reproducibility and long-term stability, as well as satisfactory practical ability in real blood serum samples.
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Analytical Methods
·
2021
·Vol. 13
·期 35
·3994-4000
·
IF 2.896
Xiao-Yang Zhao,Jia Wang,Qi-Shan Yang,Dong-Lei Fu,Dao-Kuan Jiang
Tryptophan (Trp) is one of the essential amino acids, which plays important roles in biological systems and the normal growth of human beings, and it is of great significance to be able to detect Trp in a rapid, efficient, and sensitive way. Herein, a 3D network metal–organic framework ([Sm2(BTEC)1.5(H2O)8]·6H2O) with excellent thermal and water stability was synthesized by a hydrothermal method. Interestingly, it could discriminate Trp from other natural amino acids in aqueous solution through a significant fluorescence enhancement effect, and showed high detection sensitivity (LOD = 330 nM) and outstanding anti-interference ability. The sensor system was successfully applied to the detection of Trp in practical samples, so it was expected to be a sensitive and efficient Trp sensor. In addition, the sensing mechanism was explained in detail by a series of characterization methods combined with density functional theory (DFT). There were many coordination water molecules in the crystal structure of the complex. Based on the small steric hindrance and molecular structure of water molecules, it provided the possibility for coordination interaction between Trp and Sm3+. On the other hand, the triplet energy level (T1) of Trp matched with the 4G5/2 vibrational energy level of Sm3+, so Trp could be used as the second “antenna molecule” besides 1,2,4,5-benzenetetracarboxylic acid (H4BTEC). Therefore, it effectively broadened the way for Sm-MOF to absorb excitation light.
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ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Hanyu Wang,Yukun Ouyang,Wenjing Zou,Xingchong Liu,Haimin Li,Ruonan Zhou,Xian Peng,Xiaoli Gong
Perovskite defect passivation with molecule doping shows great potential in boosting the efficiency and stability of perovskite solar cells (PSCs). Herein, an efficient and low-cost bifunctional Lewis base additive d-tryptophan is introduced to control the crystallization and growth of perovskite grains and passivation defects. It is found that the additive doped in the solution precursors could retard crystal growth by increasing activation energy, resulting in improved crystallization of large grains with reduced grain boundaries, as well as inhibiting ion migration and PbI2 aggregation. As a result, the PSCs incorporated with d-tryptophan additives achieve an improved power conversion efficiency from 18.18 to 21.55%. Moreover, the d-tryptophan passivation agent improves the device stability, which retains 86.85% of its initial efficiency under ambient conditions at room temperature after 500 h. This work provides Lewis base small-molecule d-tryptophan for efficient defect passivation of the grain boundaries toward efficient and stable PSCs.
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DALTON TRANSACTIONS
·
2022
·Vol. 51
·期 47
·18024-18032
·
IF 4.569
Guangjian Liao,Fuming Mei,Zhuqi Chen,Guochuan Yin
Dioxygen activation and catalysis around ambient temperature is a long-standing challenge in chemistry. Inspired by the significant roles of the hydrogen bond network in dioxygen activation and catalysis by redox enzymes, this work presents a Lewis acid improved dioxygen activation by an FeII(BPMEN)(OTf)2 complex towards tryptophan 2,3-dioxygenase (TDO) activity for 3-methylindole and common olefinic CC bond oxygenation and cleavage (enzymatic Brønsted acid vs. chemical Lewis acid). It was found that the presence of a Lewis acid such as Sc3+ could substantially improve olefinic CC bond oxygenation and cleavage activity through FeII(BPMEN)(OTf)2 catalyzed dioxygen activation. Notably, a more negative ρ value in the Hammett plot of para-substituted styrene oxygenations was observed in the presence of a stronger Lewis acid, disclosing the enhanced electrophilic oxygenation capability of the putative iron(III) superoxo species through its electrostatic interaction with a stronger Lewis acid. Thereof, this work has demonstrated a new strategy in catalyst design for dioxygen activation and catalysis for olefin oxygenation, a significant process in the chemical industry.
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ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Zhenying Xu,Yuanyuan Liu,Mingrui Lv,Xiujuan Qiao,Gao-Chao Fan,Xiliang Luo
The challenge of heavy biofouling in complex sweat environments limits the potential of electrochemical sweat sensors for noninvasive physiological assessment. In this study, a novel semi-interpenetrating hydrogel of PSBMA/PEDOT:PSS was engineered by interlacing PEDOT:PSS conductive polymer with zwitterionic PSBMA network. This versatile hydrogel served as the foundation for developing an anti-fouling wearable molecular imprinting sensor capable of sensitive and robust detection of tryptophan (Trp) in complex sweat. The incorporation of PEDOT:PSS conductive polymer into the semi-interpenetrating hydrogel introduced diverse physical crosslinks, including hydrogen bonding , electrostatic interactions , and chain entanglement. This incorporation considerably boosted the hydrogel's mechanical robustness and imparted commendable self-healing property. At the same time, the synergistic coupling between the well-balanced charge of the zwitterionic network and the high conductivity of the PEDOT:PSS polymer facilitated efficient charge transfer. The formation of the desired molecular imprinting membrane of semi-interpenetrating hydrogel was triggered by self-polymerization of dopamine (DA) in the presence of Trp. The designed biosensor demonstrated good sensitivity, selectivity and stability in detecting the target Trp. Notably, it also exhibited exceptional anti-fouling abilities, allowing for accurate Trp detection in complex real sweat samples, yielding results comparable to commercial enzyme-linked immunoassay (ELISA).
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