JOURNAL OF MATERIALS SCIENCE
·
2017
·Vol. 52
·期 18
·11109-11119
·
IF 2.599
Zhu Hong,Li Rui,Wang Fanghui,Chen Nanjun,Li Ziming,Wang Zhiqian
To improve the conductivity of anion exchange membranes without reducing the mechanical properties, a series of poly ionic liquids (PILs) with precursors of 1,3,5-tris(1-imidazolyl)benzene (TIB) containing tri-conduct groups were synthesized and incorporated into quaternized poly (2,6-dimethyl phenylene oxide) (QPPO) membranes. The new poly ionic liquids we designed contain ternary conduct groups in one monomer which are advantageous to form ionic channels and thus dramatically increase the conductivity of membranes. Various alkyl chains were introduced into TIB during polymerization to tune the distance between two tri-conduct groups. The resulting PILs/QPPO composite membranes showed good thermal stability, appropriate mechanical properties, enhanced conductivity and fuel cell performance. When the numbers of –CH 2 – between two tri-conduct groups reached 12, the composite membrane showed the highest conductivity and fuel cell performance (55.5 mS/cm at 80 °C and 168.9 mW/cm 2 ). The composite membranes we prepared show potential application in anion exchange membrane fuel cells (AEMFCs).
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高影响因子
DALTON TRANSACTIONS
·
2017
·Vol. 46
·期 32
·10630-10634
·
IF 4.029
Mei-Yan Gao,Shumei Chen,Lan-Xia Hu,Lei Zhang,Jian Zhang
Four titanium-oxo-clusters with cyclohex-3-ene-1-carboxylate (Cec) ligands, Ti6(μ2-O)2(μ3-O)2(Cec)4(OiPr)12 (PTC-91; HOiPr = isopropanol), Ti6(μ3-O)6(Cec)6(OiPr)6 (PTC-92 and PTC-93) and H2[Ti18(μ2-O)11(μ3-O)14(Cec)20(OiPr)4] (PTC-94), have been solvothermally synthesized under different conditions, respectively. These clusters with the same Cec ligand show totally different structures, and they are stable in water and other common solvents. The Ti6 clusters with different conformations are presented in PTC-91, PTC-92 and PTC-93, while PTC-94 is an unusual Ti18 cluster. The photocatalytic studies reveal that PTC-91 and PTC-94 have good H2 evolution ability.
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BIOELECTROCHEMISTRY
·
2022
·
IF 5.76
Xianghui Li,Yichan Huang,Jiawen Chen,Shuangmu Zhuo,Zhenyu Lin,Jianxin Chen
A sensitive homogeneous electrochemiluminescence (ECL) biosensor for flap endonuclease 1 (FEN1) detection was developed by combining highly sensitive ECL detection, high efficiency of branched hybridization chain reaction (BHCR) amplification, a convenient homogeneous strategy, and simple ultrafiltration separation. Magnetic beads were first modified with well-designed double flap DNAs containing 5′-flaps. In the presence of FEN1, the 5′-flap can be cleaved, and a large amount of single-stranded DNA can be produced, which can be separated easily from the double-flap DNA-modified beads by a magnet. Then, the cleaved 5′-flap can be used to initiate BHCR amplification to produce a large amount of long-strand dsDNA. Ru(phen) 3 2+ can insert dsDNA to form Ru-dsDNAs, which can be easily separated from the main solution through ultrafiltration. The ECL signal from the separated Ru-dsDNAs has a good linear relationship with the logarithm of the FEN1 concentration ranging from 6.5 × 10 −2 ∼ 6.5 × 10 3 U/L with a detection limit of 2.2 × 10 −2 U/L. The proposed biosensor was used to evaluate FEN1 activity in real samples with satisfactory results.
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Journal of Cell Communication and Signaling
·
2024
·
IF 3.6
Le Cao,Weilu Gao,Haitao Yang,Ran Zeng,Zongsheng Yin
Inflammation promotes the degradation of the extracellular matrix, which contributes to the development of osteoarthritis (OA). Adipocyte enhancer binding protein 1 (AEBP1) participates in multiple pathological processes related to inflammatory diseases. However, the role of AEBP1 in OA development is unknown. We found a higher AEBP1 expression in articular cartilage of OA patients ( n = 20) compared to their normal controls ( n = 10). Thus, we inferred that AEBP1 might affect OA progression. Then mice with destabilization of the medial meniscus (DMM) surgery and chondrocytes with IL-1β treatment (10 ng/mL) were used to mimic OA. The increased AEBP1 expression was observed in models of OA. AEBP1 knockdown in chondrocytes reversed IL-1β-induced inflammation and extracellular matrix degradation, which was mediated by the inactivation of NF-κB signaling pathway and the increased IκBα activity. Co-immunoprecipitation assay indicated the interaction between AEBP1 and IκBα. Importantly, IκBα knockdown depleted the protective role of AEBP1 knockdown in OA. Moreover, AEBP1 knockdown in mice with OA showed similar results to those in chondrocytes. Collectively, our findings suggest that AEBP1 knockdown alleviates the development of OA, providing a novel strategy for OA treatment.
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PubMed
高影响因子
Agronomy-Basel
·
2022
·Vol. 13
·期 1
·46
·
IF 3.949
Han Yan,Rui Wang,Ning Ji,Jiangkuo Li,Chao Ma,Jiqing Lei,Liangjie Ba,Guangzhong Wen,Xiaobo Long
In order to study a short-term and efficient technology by 1-methylcyclopropene (1-MCP) in blueberry, the fruit was treated with 0, 0.5, 1 and 3 μL/L 1-MCP for 2 h then stored at 25 ± 1 °C with 40–50% relative humidity (RH) for 9 d. The weight loss, decay incidence, respiration rate, firmness, soluble solid content (SSC), titratable acid (TA), Brix-acid ratio (BAR), sensory evaluation, content of cell wall polysaccharide, activities of cell wall composition-related enzymes and energy metabolism in blueberry were determined during shelf life. The results showed that the weight loss, decay incidence and respiration rate were reduced by 3 μL/L 1-MCP treatment. Compared to other groups, the firmness, the content of TA and anthocyanins were maintained in 3 μL/L 1-MCP-treated blueberry. In contrast, the SSC and BAR were lower compared to those untreated. However, the sensory evaluation of “taste” and “aroma” value showed no differences in all fruits. The content of protopectin, cellulose and hemicellulose was higher in 1-MCP-treated blueberry, accompanied by a decrease in polygalacturonase (PG) and pectin methyl esterase (PME) activity. The content of water-soluble pectin (WSP) was lower in 1-MCP-treated blueberry than untreated ones. The activity of phenylalanine ammonia lyase (PAL), peroxidase (POD), cinnamyl alcohol dehydrogenase (CAD) and 4-coumarate-CoA ligase (4CL) was higher in 1-MCP-treated blueberry than the untreated, which induced more serious lignification. The results of energy metabolism also showed that the 1-MCP treatment could ensure sufficient intracellular energy supply. The 3 μL/L 1-MCP treatment could maintain the shelf quality and retard decomposition of cell wall polysaccharide by ensuring sufficient intracellular energy supply and inhibiting cell wall-degrading enzymes activity. Taken together, this study highlighted an efficient and short-term 1-MCP treatment technique.Keywords:blueberry;1-MCP;cell wall polysaccharide;shelf life;karst fruit
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Cell and Bioscience
·
2025
·Vol. 15
·期 1
·159
·
IF 6.2
Sun Weikang,Zhang Mengyu,Wang Ruilin,Yang Jie,Rasool Ameena Tur,Luo Renjie,Liu Xiangdong,Cao Peng,Li Erguang
Background The RNA-binding protein polypyrimidine tract-binding protein 1 (PTBP1), also known as heterogeneous nuclear ribonucleoprotein I (hnRNP I), mediates gene expression through splicing regulation. Its role in virus infection is undefined. Results We show that genetic ablation of PTBP1 renders cell resistant to herpes simplex virus 1 (HSV-1) infection. HSV-1 utilizes 3-O-sulfated heparan sulfate proteoglycans (HSPGs) for attachment and for infection of epithelial cells. We found that knockout of PTBP1 expression resulted in loss of HS3ST3A1 and HS3ST3B1, heparan sulfate glucosaminyl 3-O-sulfotransferase genes for 3-O-sulfation of the heparan sulfate (HS) chains of HSPGs. Each of the HS3ST3A1/HS3ST3B1 genes is composed of 2 exons separated by an extraordinarily long intron whose removal requires PTBP1-associated looping. We found that PTBP1 interacted with the intronic region of HS3ST3A1/HS3ST3B1 pre-mRNAs and modulated their processing to mRNA. The essential role of PTBP1 in functional HS3ST3A1 expression and in HSV-1 infection was demonstrated by ectopic re-expression in the knockout (ko) cells. In addition, we showed that targeting PTBP1 expression by microRNA mimics reduced disease symptoms in a mouse herpetic stromal keratitis (HSK) model. Conclusions The results demonstrate that PTBP1 mediates HSV-1 infection of epithelial cells through splicing regulation of HS3ST3A1/HS3ST3B1. These studies provide a new area for novel therapeutic strategies through splicing regulation. Graphical abstract By Sun et al. RNA-binding protein PTBP1 mediates HSV-1 attachment and infection through regulation of heparan sulfate 3-O-sulfotransferase gene expression.
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PubMed
高影响因子
PHARMACEUTICAL BIOLOGY
·
2025
·
IF 4.8
Yong-xiang Huang,Jin-ru Zhang,Shi-hua Fu,Zhe Liu,Hui-jun Zhong,Qing-yang Zhong,Yin-di Luo,Ying-jie Hu,Hai-peng Zhu,Bin Wen,Song Chen
Context Macrophages play a critical role in the pathogenesis of ulcerative colitis (UC). Indirubin (IDR), a natural ligand of the aryl hydrocarbon receptor (AhR), has been shown to ameliorate DSS-induced colitis in our previous study (Liu Z et al.).Objective To investigate whether IDR exerts its protective effects by regulating M1/M2 polarization and inhibiting ferroptosis in macrophages.Materials and Methods Immunohistochemistry staining targeting CD206 and F4/80 was performed to evaluate the effect of IDR on the polarization of M1/M2 macrophages in colitis. Subsequently, the effects of IDR on the M1- or M2-polarization of THP-1-derived macrophages were investigated. Furthermore, the effects of IDR on ferroptosis in the colon tissue of mice and on RSL3-induced ferroptosis in THP-1-derived macrophages were assessed. The results were verified in mouse peritoneal macrophages.Results In addition to reducing the infiltrated macrophages, IDR treatment preserved CD206+ macrophages in DSS-induced colitis. Using cultured THP-1 cells, we demonstrated that IDR inhibited M1 polarization and prompted M2 polarization. Furthermore, we showed that IDR treatment decreased levels of 4-HNE while increasing GPX4 and NRF2 in DSS-induced colitis and THP-1 cells. IDR treatments also reduced cellular reactive oxygen species (ROS) and iron content, and mitigated RSL3-induced ferroptosis in THP-1-derived macrophages. Similarly, IDR augmented M2-polarization and alleviated ferroptosis in peritoneal macrophages.Discussion and Conclusion IDR skews the polarization of macrophages from M1 to M2. Furthermore, it inhibits ferroptosis in both mice and THP-1-derived macrophages. These mechanisms may contribute to the therapeutic effects of IDR in the treatment of UC.
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PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Jiawei Ruan,Xiaoyi Zhang,Wanting Zhang,Xinzi Wu,Qian Liu,Zhen Song,Lifang Chen,Zhiwen Qi
The direct synthesis of dimethyl carbonate (DMC) from CO 2 is a promising route for CO 2 utilization, however it is constrained by high CO 2 activation barrier and inherent thermodynamic limitations. Herein, we developed a reactive extraction strategy to intensify the reaction through 1-alkyl-3-methylimidazolium triazolide ionic liquids (ILs) (CnMImTz, n = 2, 4, 6, and 8) by modulating IL polarity. The absorption and activation mechanism of CO 2 in ILs was demonstrated, and subsequent transformation into DMC can be achieved under mild conditions. Remarkably, C2MImTz achieves the highest DMC yield (9.4 % after 12 h at 25 °C), revealing chain-length dependent catalytic efficiency of IL analogs at both kinetics- and thermodynamic-controlled stages. Phase analyses, binding energy calculations, and molecular dynamics simulations revealed that the highly polar C2MImTz preferentially concentrate CH 3 OH and H 2 O, while spontaneously segregating weakly polar DMC into a separate phase. The enhanced CH 3 OH concentration and diffusion coefficients promote the mass transfer and reaction kinetics. Moreover, the segregation of DMC can effectively shift thermodynamic equilibrium toward DMC formation. Therefore, the integrated absorption-conversion-separation process within a single functionalized IL presents a scalable platform for CO 2 capture and utilization.
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高影响因子
Catalysts
·
2025
·Vol. 15
·期 4
·390
·
IF 4
Zezheng Bing,Yuanyuan Gao,Zhongyi Liu,Qiaoyun Liu
Biomass, as a renewable carbon resource, holds broad application prospects. Among various bio-based platform molecules, furan derivatives play a significant role in green chemical production. Notably, the conversion of 2-methylfuran (2-MF) to 3-acetyl-1-propanol (3-AP) over bifunctional catalysts has attracted considerable interest. In this study, a Pd@PHZSM-5 catalyst was prepared by encapsulating Pd nanoparticles within P-doped HZSM-5 for 2-MF conversion. The encapsulation improved Pd dispersion and metal–acid synergy, enhancing both catalytic activity and 3-AP selectivity. Additionally, phosphorus doping increased HZSM-5 crystallinity, resulting in excellent stability. This work provides a feasible strategy for optimizing metal–acid cooperation, offering theoretical guidance for bifunctional catalysis and biomass valorization.
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高影响因子
HISTOCHEMISTRY AND CELL BIOLOGY
·
2025
·Vol. 163
·期 1
·1-15
·
IF 2.1
Zhong Wen-Jing,Xiong Jian-Bing,Zhang Chen-Yu,Jin Ling,Yang Nan-Shi-Yu,Sha Han-Xi,Liu Yu-Biao,Duan Jia-Xi,Guan Cha-Xiang,Zhou Yong,Su Feng
Pulmonary fibrosis (PF) is an insidious, progressive, and fatal age-associated disease that occurs primarily in older adults and has a poor prognosis. Alveolar epithelial cell (AEC) senescence is the critical pathological mechanism of PF. The accumulation of oxygen radicals, commonly referred to as reactive oxygen species (ROS), strongly contributes to cellular senescence. The triggering receptor expressed on myeloid cells-1 (TREM-1) is a pattern recognition receptor. Triggering via TREM-1 results in ROS, leading to the amplification of inflammation. However, whether TREM-1 is involved in PF by inducing oxidative stress to exacerbate AEC senescence remains unclear. We first observed that blockade of TREM-1 during the fibrotic phase attenuated bleomycin (BLM)-induced PF in mice, with decreased expression of senescence-related proteins, including p16, p21, p53, and γ-H2AX, in the lung tissue. Moreover, TREM-1 blockade during the fibrosis stage restored antioxidant levels by increasing the percentage of Nrf2- and HO-1-positive cells in mice with PF. Notably, TREM-1 was highly expressed in surfactant-associated protein (SPC)-positive AECs in mice with PF. In vitro, blocking TREM-1 activated Nrf2 antioxidant signaling, thereby decreasing intracellular ROS levels and diminishing BLM-induced senescence in AECs. Furthermore, inhibition of Nrf2/HO-1 partially counteracted the anti-senescence effect of blocking TREM-1 in BLM-treated AECs. In this study, we reported that TREM-1 stimulated the senescence of AECs, induced ROS and exacerbated PF. We also provide compelling evidence suggesting that the Nrf2/HO-1 signaling pathway underpins TREM-1-triggered senescence. Therefore, our findings provide new insights into the molecular mechanisms associated with TREM-1 and AEC senescence in the pathogenesis of PF.
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PubMed
高影响因子
FUEL
·
2024
·
IF 6.7
Zunlong Hu,Peng Hu,Xiulin Wang,Tongtong Wu,Sida Ge,Haodong Xie,Baijun Liu,Shengli Chen,Zhijie Wu
The hydrocracking catalyst is characteristic of bifunctional catalyst mainly requiring metal–acid balance, which includes metal–acid ratio and metal–acid proximity. Here, we screened and clarified the optimal metal–acid balance on 1-methylnaphthalene (1-MN) hydrocracking by adjusting metal–acid ratio (n M /n A ) via physically mixing the γ-Al 2 O 3 supported NiW samples and Beta zeolite with different acidity (denoted as NiW/AB(x) and x presents the molar ratio of SiO 2 /Al 2 O 3 for Beta zeolite). NiW/AB(60) catalyst with a n M /n A of 0.40 showed the highest BTX selectivity (14.4 %) at a 1-MN conversion of 76.6 %, suggesting an optimal site balance between the metal sulfide site and medium-strong acid sites ( ca . 1:2.5). Also, four different metal–acid proximities with centimeter, millimeter, micrometer and nanoscale distances were compared. The nanoscale proximity enabled the highest BTX selectivity (19.6 %) as compared to the microscale (14.4 %), millimeter scale (12.4 %) and centimeter scale (7.1 %) catalysts, confirming that the selectivity of BTX benefited from the fast diffusion for transporting reaction intermediates between metal and acid sites. Thus, we realized a n M /n A of 0.40 and a nanoscale site proximity for improving the reactivity and selectivity of 1-MN hydrocracking. This work not only presents an elegant showcase of achieving the synergistic effects of bifunctional catalyst, but also provides a rational approach for enhancing the catalytic performance of hydrocracking and beyond.
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高影响因子
MACROMOLECULAR RAPID COMMUNICATIONS
·
2022
·Vol. 44
·期 6
·2200826
·
IF 5.006
Jiaqi Li,Weilin Feng,Mengxiao Zhang,Xiaohe Wang,Chuanjie Fang,Jianyu Wang,Lin Zhang,Liping Zhu
Polymers of intrinsic microporosity (PIMs) are a class of microporous organic materials that contain interconnected pores of less than 2 nm in diameter. Such materials are of great potential used in membranes for molecular separation, such as drug fractionation in pharmaceutical industry. However, the PIMs membranes are often susceptible to low separation selectivity toward different molecules due to their wide pore size distribution. Herein, a linear polyimide, Matrimid, is incorporated with PIM-1 (a typical member of PIMs) by solution blending, and the blends are dip-coated onto a polyimide P84 support membrane to prepare thin-film composite (TFC) membranes to control pore size distribution while keep high microporosity. The component miscibility, pore characteristics, and molecular separation performances of the Matrimid/PIM-1 TFC membranes are investigated in detail. The Matrimid and PIM-1 are partially miscible due to their similar Hansen solubility parameters. The Matrimid endows the selective layers (coatings) with narrower pore size distribution due to more compact chain packing. The prepared Matrimid/PIM-1 TFC membranes show high selectivity for separation of riboflavin (80% of retention) and isatin (only 5% of retention). The developed membranes exhibit great potential for separating molecules with different molecular weights.
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高影响因子
危险品化学品经营许可证（带存储）