POLYMER INTERNATIONAL
·
2020
·Vol. 69
·期 10
·985-994
·
IF 2.574
Meng Ma,Xinpeng Wang,Kai Liu,Si Chen,Yanqin Shi,Huiwen He,Xu Wang
The preparation of poly( l -lactic acid) (PLLA) with high mechanical and ideal flame-retardant properties is a great challenge. Herein, a simultaneous toughness and flame-retardant PLLA composite was successfully fabricated by using a one-step process which introduces 4,4′-methylenediphenyl diisocyanate and ammonium polyphosphate (APP) into PLLA/poly(ε-caprolactone) blends. SEM, Fourier transform infrared spectroscopy and TGA were adopted to confirm that APP participated in the in situ reaction during the melt process. The impact strength was increased to 13.5 kJ m −2 from 1.0 kJ m −2 for L8P2A5 composite, indicating the toughening effect of reactive blending. The cone calorimeter test, limiting oxygen index and vertical burning test results indicate that the flame-retardant properties of the composites are enhanced with increasing APP content. This work provides a method to prepare PLLA with high mechanical properties and enhanced flame retardancy. © 2020 Society of Chemical Industry
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ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Yingcong Zhang,Ze Zhang,Shengzhong Rong,Hongwei Yu,Hongmin Gao,Qian Sha,Ping Ding,Hongzhi Pan,Dong Chang
An ultrasensitive sandwich-type electrochemiluminescence (ECL) immunosensor was developed for detection of prostate specific antigen (PSA) using an amplification strategy based on ZnO nanorods- l -cysteine-luminol nanocomposites and the biotin-streptavidin system. The biotin-streptavidin system served as a capture probe to increase the number of antibodies. ZnO nanorods not only acted as a nanocarrier that increased the number of luminol molecules and secondary antibodies, but also enhanced the ECL signal of luminol-H 2 O 2 system by promoting H 2 O 2 decomposition, which can further increase ECL intensity. Under optimized conditions, the proposed immunosensor demonstrated excellent analytical performance with a wide linear detection range of 0.03 pg mL −1 to 30 ng mL −1 and a detection limit of 0.01 pg mL −1 (the detection limit in real samples was 0.021 pg mL −1 ). This method exhibited excellent stability, reproducibility, and selectivity. In addition, the results of PSA determinations in human serum samples obtained using the proposed immunosensor were consistent with data collected using the commercial chemiluminescence immunoassay analyzer.
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BIORESOURCE TECHNOLOGY
·
2021
·
IF 9.642
Qian Feng,Laiwei Luo,Xindi Chen,Kaijie Zhang,Fang Fang,Zhaoxia Xue,Chao Li,Jiashun Cao,Jingyang Luo
The N-Acy-L-homoserine lactones (AHLs) mediated quorum sensing (QS) system exhibited important ecological significance in bacterial biofilm formation. However, the previous studies mainly focused on indigenous AHLs while the role of exogenous AHLs has remained unclear. This study evaluated the roles of exogenous AHLs on the biofilm formation of Pseudomonas aeruginosa . Both the C6-HSL and C8-HSL promoted the biofilm formation of P. aeruginosa with an enhancement of 2.47 and 1.88 times, respectively. Further analysis showed that exogenous AHLs contributed greatly to the adhesive ability instead of growth rate. Also, the bacterial motility and metabolic activities were significantly improved by AHLs. Moreover, the microbial functional genes ( i.e. lasI , lasR , rhlI and rhlR ) involved in regulating the biofilm formation were highly expressed in AHLs reactors. These findings expanded the knowledge of AHLs functions in mediating biofilm formation, and provided insightful guidance on the biofilm regulation in the wastewater treatment via biofilm technology.
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JOURNAL OF FISH BIOLOGY
·
2021
·Vol. 99
·期 6
·1843-1856
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IF 2.051
Liping Yang,Shaoyang Zhi,Guokun Yang,Chaobin Qin,Xiao Yan,Mingming Niu,Wenlei Zhang,Mingyu Liu,Mengjuan Zhao,Guoxing Nie
Glucose transporter 4 (GLUT4) is comprehensively investigated in mammals, while the comparative research of GLUT4 in common carp is deficient. To investigate the function of GLUT4, carp glut4 was first isolated. The open reading frame of carp glut4 was 1518 bp in length, encoding 505 amino acids. A high-sequence homology was identified in carp and teleost, and the phylogenetic tree displayed that the carp GLUT4 was clustered with the teleost. A high level of glut4 mRNA was analysed in fat, red muscle and white muscle. After fasting treatment, glut4 mRNA expression was increased significantly in muscle. In the oral glucose tolerance test experiment, glut4 mRNA was also significantly elevated in muscle, gut and fat. Furthermore, intraperitoneal injection of insulin resulted in the upregulation of glut4 gene expression significantly in white muscle, gut and fat. On the contrary, the glut4 mRNA level in the white muscle, gut and fat was markedly downregulated after glucagon injection. These results suggest that GLUT4 might play important roles in food intake and could be regulated by nutrient condition, insulin and glucagon in common carp. Our study is the first to report on GLUT4 in common carp. These data provide a basis for further study on fish GLUT4.
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Journal of Oleo Science
·
2021
·
IF 1.601
Fang-Xiang Xu,Jing-Yu Zhang,Jing Jin,Zu-Guang Li,Yuan-Bin She,Maw-Rong Lee
In the past decade, natural deep eutectic solvents (NADESs) as green and sustainable extraction solvents with great potential for the efficient extraction of bioactive compounds from the plants are emerging. In this study, a microwave-assisted technology is used to prepare natural deep eutectic solvents. And natural deep eutectic solvents as pretreatment solvents coupled with microwave-assisted hydrodistillation (MAHD) for isolating essential oil (EO) derived from turmeric (Curcuma longa L.) is investigated. To improve the essential oil yield of turmeric (Curcuma longa L.) as a target, various factors affecting extraction efficiency including the type and amount of natural deep eutectic solvents, pretreatment time, pretreatment temperature and hydrodistillation (HD) time are discussed and optimized through central composite design (CCD) of the response surface methodology (RSM). The optimal conditions are as follows: natural deep eutectic solvent composed of choline chloride and oxalic acid (molar ratio with 1:1) as a pretreatment solvent, an amount of 60 g, a pretreatment time of 5 min, a pretreatment temperature of 84 ºC, a hydrodistillation time of 76 min. Under the optimum conditions, the highest essential oil yield of 0.85% is achieved. Additionally, the essential oil is analyzed by using gas chromatography-mass spectrometry (GC-MS), with a total of 49 compounds being identified. Through combining natural deep eutectic solvents with a microwave-assisted hydrodistillation technique, this work provides an eco-friendly extraction way of isolating essential oil, which boosts development in the monitoring other spice quality field.
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CELLULOSE
·
2019
·Vol. 26
·期 17
·9323-9333
·
IF 3.917
Zhou Jing,Hu XinYue,Zhu YiYuan,Lyu HongFen,Zhang Lei,Fu FeiYa,Liu XiangDong
This work proposes a facile approach to improve the durability of antibacterial cotton fabric by using carboxymethyl chitosan (CMC) and l -methionine (Met) as a combination binder to immobilize silver nanoparticles (Ag NPs). The durable antibacterial function is achieved by grafting CMC and Met molecules onto a cotton fabric by a simple pad-dry-cure process, followed by the preparation of Ag NPs on the modified surface. This surface modification process provides the cotton fabric with an excellent antibacterial effect and outstanding laundering durability. Structural analyses of the modified surface revealed that the covalent bonds between Met, CMC, and cotton fibers were formed, and the thioether groups caused the stable immobilization of the Ag NPs. Notably, the modification process does not significantly damage the original fiber structure. The desired cotton properties such as vapor permeability, water absorptivity, and flexibility of the modified fabric were very close to the original cotton. Moreover, the safety to human skin was verified by cytotoxicity tests using fibroblast cells.
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CHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 8.355
Zhao-Hui Zhang,Jian-Wang Zhang,Cheng-Fei Cao,Kun-Yu Guo,Li Zhao,Guo-Dong Zhang,Jie-Feng Gao,Long-Cheng Tang
Frequent fire disasters have not only caused massive casualties, irreparable property and priceless artefacts loss, but also create severe environmental damage. Therefore, it is imperative, but also challenging, to obtain excellent fire resistance and efficient early-warning alarm response in precombustion of combustible materials. In this work, a green flame-retardant graphene oxide (GO) based paper/coating is designed and constructed via using a facile 3-methacryloxypropyltrimethoxysilane and L-ascorbic acid (LAA) co-functionalization strategy. The optimized co-modified GO network shows good structure stability and improves the flame retardancy of combustible materials. Further, such GO network can provide an ultrafast flame detection signal of only ~1 s and ideal fire early warning responses (e.g. a low responsive temperature of ~120 °C and an extremely rapid responsive time of ~7 s at 300 °C) in precombustion which are among the best performances of the state-of-the-art fire alarm sensors. Moreover, the transformation of silane molecules into a compact protective layer on sheets and the resistance transition of insulating GO network into conductive reduced GO path promoted by LAA molecules are clarified. This work provides a novel strategy and paradigm to achieving excellent flame resistance and ideal fire early warning response of GO network for fire safety and prevention applications.
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BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Caixia Wang,Liming Chen,Peijin Wang,Mengsi Li,Defang Liu
In this work, an ultrasensitive electrochemiluminescence (ECL) biosensor was constructed using poly- L -lysine (PLL) as a novel co-reactant of luminol and poly(luminol/aniline) nanorods loaded reduced graphene oxide ( [email protected] ) as nanoprobe, which enable highly sensitivity detection of glutathione (GSH). To the best of our knowledge, it is the first time that PLL was used for the co-reactant of luminol. Notably, about a 5-fold enhancement was obtained compared with the individual [email protected] using GCE . Due to the remarkable quenching effect between the excited state of PLL and the reduced form of GSH in the ECL system of luminol/PLL, the ECL sensing platform exhibited wide linear ranges of 1.0 × 10 −9 –1.0 × 10 −4 M and 1.0 × 10 −4 –1.0 × 10 −2 M and a low detection limit of 7.7 × 10 −10 M. Simultaneously, the biosensor was also successfully applied to detect GSH in human serum sample with high recoveries. Hence, this work would open a new platform for the wide application of PLL in immunoassay and various sensors.
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MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 7
·1-8
·
IF 5.705
Wen Haibin,Jiang Peichun,Hu Yuling,Li Gongke
Poly(3,4-dihydroxy-L-phenylalanine) (polyDOPA) is a stable and biocompatible reducing agent. A versatile strategy is described here for the synthesis of core-shell Au@Ag nanostructures containing a polyDOPA interlayer. The latter provides abundant sites for deposition of nanocomposites, to immobilize molecules and to grow shells. The Au@polyDOPA@Ag nanoparticles are shown to generate strong and stable surface-enhanced Raman spectroscopy (SERS) signals compared to bare AuNPs and bare AgNPs. Folic acid was then immobilized on Au@polyDOPA@Ag nanoparticles and then applied to SERS imaging of human lung adenocarcinoma cell line A549 by the specific recognition of the folic acid receptor. The folic acid-conjugated SERS tags were promising to be nanoplatforms for imaging of cancer cells.
An Au@Ag core-shell nanostructures SERS nanotag with a polyDOPA interlayer was fabricated and then applied to SERS imaging of epithelial cells. (DOPA: 3,4-Dihydroxy-[ L -phenylalanine]; FA: folic acid; 4-MBA: 4-mercaptobenzoic acid).
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FISH & SHELLFISH IMMUNOLOGY
·
2018
·
IF 3.185
Dong-Liang Lu,Samwel Mchele Limbu,Hong-Bo Lv,Qiang Ma,Li-Qiao Chen,Mei-Ling Zhang,Zhen-Yu Du
Dietary α-lipoic acid (LA), β-glucan (Gluc) and l -carnitine (L-Ca) are commonly used additives to promote fish growth and stress resistance in aquaculture production. However their mechanisms and efficiencies in helping fish to resist diseases have not been compared before. In this study, we fed Nile tilapia ( Oreochromis niloticus ) with diets containing appropriate doses of LA, Gluc and L-Ca for five weeks and further intraperitoneally injected the fish with Aeromonas hydrophila . After dietary treatment, none of the additives affected the fish growth, but dietary Gluc and L-Ca reduced protein and lipid body contents in fish, respectively. After A. hydrophila challenge, all fish treated with the three dietary additives showed higher survival rate, but those fed on dietary L-Ca had lower survival than those fed on LA and Gluc diets, indicating high protection efficiency of LA and Gluc. The protective mechanisms of the three feed additives were quite different under A. hydrophila infection. Dietary LA induced higher total antioxidant capacity and higher mRNA expression of anti-oxidative genes than other additives in liver and also activated partly the immune function in serum and spleen. Gluc largely increased the immune function by activating the immunity enzymes in serum, inducing inflammation in liver and increasing the expression of immune genes in spleen and head kidney . Gluc also increased partly the antioxidant capacity in serum and liver and lipid catabolism in liver. L-Ca largely increased lipid catabolism in liver while it increased partly the antioxidant capacities in serum and liver. Taken together, these results indicate that, dietary LA, Gluc and L-Ca have various protective mechanisms and differ in their efficiencies on resisting A. hydrophila infection in Nile tilapia.
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Polymer Chemistry
·
2015
·Vol. 6
·期 38
·6857-6869
·
IF 5.52
Xingjie Wu,Linzhu Zhou,Yue Su,Chang-Ming Dong
A series of comb-like graft polypeptides having different side groups and tunable grafting ratios were prepared by sequential photocleavage reactions and Michael-type thiol–ene addition, as fully characterized by 1H NMR, gel permeation chromatography, FT-IR, and circular dichroism spectroscopy. The grafting ratio of the resulting polypeptides was close to the photolysis ratio of o-nitrobenzyl derived poly(L-cysteine) precursors. Both electrolytic acrylic acid (AA) and 2-(dimethylamino) ethyl acrylate (DMAEA) produced a larger disturbance on the ordered conformations than neutral poly(ethylene glycol) (PEG) methyl ether acrylate although the molecular weight and size of AA and DMAEA are much smaller than those of PEG. The polypeptide vesicles and micelles with a neutral or electrolytic corona could be facilely fabricated from these side group modified poly(L-cysteine)s, and the polypeptide vesicles exhibited both light- and redox-sensitivity in aqueous solution. As characterized by the MTT assay, flow cytometry, and CLSM, the anticancer drug doxorubicin (DOX) loaded nanoparticles quickly entered into HeLa cells and presented photo- and reduction-triggered cytotoxicity. The half maximal inhibitory concentration of HeLa cells (IC50) for the irradiated nanoparticles dropped to 0.40 μg DOX equiv. per mL compared to 1.27 μg DOX equiv. per mL for the non-irradiated sample; however, the IC50 for the irradiated sample increased about 1.5-fold after the BSO inhibitor treatment. Importantly, this work not only establishes a facile method for the preparation of comb-like graft polypeptides by the combination of photochemistry and thiol–ene click chemistry, but also provides a promising platform for on-demand nanomedicine and cancer therapy.
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POLYMER
·
2014
·
IF 3.766
Weichun Huang,Sheng Wang,Chengxin Guo,Xiaoming Yang,Yaowen Li,Yingfeng Tu
Two simple and effective methods, “click” chemistry and supramolecular interactions, are demonstrated here to synthesize well-defined poly( l -lactide) (PLLA) functionalized graphene oxide (GO) sheets. We provide a simple method to introduce azide groups on GO sheets by the ring opening reaction of sodium azide with the epoxide groups of GO. The GO-N 3 sheets can easily undergo “click” reaction with alkyne-terminated PLLA by “grafting onto” method to produce GO/PLLA composites with high grafting ratio and exfoliated structure. Interestingly, GO-N 3 can be grafted with oxygen-containing polymers such as PLLA, polymethyl methacrylate (PMMA) or polyethylene oxide (PEO) via supramolecular interactions between the azide groups and these oxygen atoms on polymers, producing GO/polymer composites with low grafting ratio and intercalated structure. These “grafting onto” methods are useful to produce a variety of GO/polymer composites with different structure via “click” reaction or supramolecular interactions, which have potential applications in material science.
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