Food and Bioprocess Technology
·
2026
·Vol. 19
·期 10
·519
·
IF 5.6
Pei Yiqiao,Yang Xu,Zhang Cheng,Sun Junna,Zhang Xiaohan,Cheng Jayden,Wang Hao,Fan Kaijie
High relative humidity (RH) in prawn storage environments accelerates microbial proliferation, resulting in a shelf life within 3 days at 4 °C. Based on this preservation environment, this study developed an antimicrobial smart film composed of cinnamon essential oil (CEO)-liposome-mussel adhesion proteins (MAP)-polyvinyl alcohol (PVA), evaluated prawn preservation effects, and explored the RH trigger mechanism. The results showed that the film containing CEO-loaded liposomes and 75 mg of MAP (F-CLP 75 ) could reduce the premature loss of CEO under relatively dry conditions. Under high-RH conditions, the film swelled and promoted CEO release while maintaining structural integrity, thereby delaying prawn quality deterioration associated with free water accumulation, microbial proliferation, and protein degradation, and extending the acceptable storage period of prawns to approximately 5–6 days at 4 °C. Additionally, compared with the blank liposome film (F-LP 0 ), F-CLP 75 exhibited significantly improved water resistance, thermal stability, mechanical properties, and barrier properties. This study provides useful guidance for the development of RH-responsive antimicrobial packaging films and the upgrading refrigerated preservation technologies for aquatic products. Graphical
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JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
·
2026
·
IF 4.1
Ren Wen-long,Huang Qian,Wang Yi-kai,Zhang Ming-xing,Guo Kai-ge,Zhang Cheng-chen,Zeng Yu-yang,Lin Zhi-wen,Xiong De-hao,Zhu Chen-guang
Potassium perchlorate (KClO 4 ), as a vital energetic oxidizer, has its application in propellants and other fields limited by its inherently high decomposition temperatures. To overcome this limitation, this study employs a freeze-drying-assisted assembly strategy to successfully synthesize ferrite/carbon nanotube (MFe 2 O 4 /CNTs, M = Cu, Co, Ni) composites. Material characterization techniques, including SEM, XRD, and XPS, confirmed the morphology, crystal structure, elemental chemical states, and surface oxygen speciation. Differential thermal analysis demonstrated that the prepared composites significantly reduce the thermal decomposition temperature of KClO 4 and substantially decrease the activation energy. Among them, CoFe 2 O 4 /CNTs exhibited the optimal catalytic performance, lowering the decomposition temperature from 615.4 to 492.6 ℃ at a heating rate of 10 ℃ min −1 . Mechanism analysis via the reaction function Z(α) revealed that the decomposition of pure KClO 4 follows a three-dimensional nucleation and growth mechanism (Avrami–Erofeev model). In contrast, the introduction of CoFe 2 O 4 /CNTs shifts the rate-controlling step to a three-dimensional diffusion mechanism (D3 model), indicating that the catalyst reshapes the decomposition pathway by accelerating the diffusion of gaseous intermediates. The enhanced catalytic performance originates from multiple synergistic effects: the redox activity of the spinel structure, the activation of oxygen species by surface oxygen vacancies, and the accelerated electron transport facilitated by the three-dimensional conductive network constructed by the CNTs. Furthermore, combustion tests of the energetic material confirmed that incorporating 3 mass% CoFe 2 O 4 /CNTs catalyst shortened the combustion time by 57.2%, expanded the flame propagation area, increased the combustion temperature, and led to a more stable combustion profile.
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CELL DEATH AND DIFFERENTIATION
·
2023
·Vol. 30
·期 5
·1260-1278
·
IF 12.4
Sun Ge,Wei Yuntao,Zhou Baosheng,Wang Manlin,Luan Ruina,Bai Yu,Li Hao,Wang Shan,Zheng Dantong,Wang Chunyu,Wang Shengli,Zeng Kai,Liu Shuchang,Lin Lin,He Mingcong,Zhang Qiang,Zhao Yue
The estrogen receptor alpha (ERα) signaling pathway is a crucial target for ERα-positive breast cancer therapeutic strategies. Co-regulators and other transcription factors cooperate for effective ERα-related enhancer activation. Recent studies demonstrate that the transcription factor CTCF is essential to participate in ERα/E2-induced enhancer transactivation. However, the mechanism of how CTCF is achieved remains unknown. Here, we provided evidence that BAP18 is required for CTCF recruitment on ERα-enriched enhancers, facilitating CTCF-mediated chromatin accessibility to promote enhancer RNAs transcription. Consistently, GRO-seq demonstrates that the enhancer activity is positively correlated with BAP18 enrichment. Furthermore, BAP18 interacts with SMARCA1/BPTF to accelerate the recruitment of CTCF to ERα-related enhancers. Interestingly, BAP18 is involved in chromatin accessibility within enhancer regions, thereby increasing enhancer transactivation and enhancer-promoter looping. BAP18 depletion increases the sensitivity of anti-estrogen and anti-enhancer treatment in MCF7 cells. Collectively, our study indicates that BAP18 coordinates with CTCF to enlarge the transactivation of ERα-related enhancers, providing a better understanding of BAP18/CTCF coupling chromatin remodeling and E-P looping in the regulation of enhancer transcription.
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ANALYTICAL SCIENCES
·
2025
·
IF 2
Xia Shaojie,Yang Zhenyuan,Sun Xiaohui,Huang Junjie,Peng Kuan,Wang Yonghong
Ochratoxin A (OTA), a highly toxic mycotoxin, extensively contaminates a wide range of food commodities. Given increasing demands for sensitive and user-friendly detection platforms, we developed a colorimetric OTA biosensor leveraging copper-silver nanoclusters (Cu/Ag NCs), whose peroxidase-like activity is augmented via G/C-rich DNA hybridization. The system employs two tailored DNA strands: a template strand featuring a cytosine-rich domain for NC synthesis flanked by terminal recognition segments and an enhancer strand containing a guanine-rich signal amplification domain and a complementary linker. Upon OTA binding with the aptamer, the apt-template complex dissociates, facilitating template-enhancer hybridization to form G/C-rich double-stranded DNA. This structural transition substantially enhances the peroxidase-like activity of DNA-Cu/Ag NCs, accelerating the oxidation of 3,3′,5,5′-tetramethylbenzidine by H 2 O 2 while simultaneously amplifying the colorimetric signal. Quantitative OTA detection is accomplished by monitoring this dynamic color response. The biosensor demonstrates a semi-log linear detection range from 5.0 nM to 1.0 μM with a detection limit of 5.0 nM. Furthermore, the specific test has high selectivity for other common mycotoxins, and its applicability has been validated through real sample analysis, suggesting that the DNA-Cu/Ag NCs biosensor used for OTA detection holds promising practical applications in food samples. Graphical abstract
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npj Science of Food
·
2026
·
IF 9.7
Li Ya,Wu Hailin,Liang Jianfeng,Pan Yuhua
HS-SPME-GC/MS and UPLC were used to profile volatiles and non-volatiles in four Liupao tea aroma types (betelnut, stale, ginseng, and jujube). Combined flavoromics and molecular docking clarified aroma differentiating metabolites and their sweet taste binding mechanisms. In total 1509 volatiles across 15 classes were detected; terpenes, esters and ketones dominated. 47 key aroma compounds (ROAV≥100) distinguishing the four types were screened via OPLS-DA. Nonvolatile catechin, gallic acid and theobromine also mediated flavor divergence, with Mantel tests revealing synergistic correlations between catechin compounds and key volatiles. The 12 volatile compounds mainly bind to the T1R2 and T1R3 sweet taste receptors by Van der Waals and electrostatic interactions. Terpenoid and amino acid metabolic pathways dominated aroma differentiation. This work identifies type specific key metabolites and their molecular flavor-forming mechanisms to support Liupao tea aroma classification.
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BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
·
2025
·Vol. 116
·期 1
·10
·
IF 2.2
Huang Junyun,Liu Boying,Yuan Sijia,Zhou Haiyun,Bao Jian,Tu Yong,Wang Zunyao,Wang Weixia
The UV filter benzophenone-3 (BP-3) is a prevalent environmental pollutant due to its extensive use in personal care products, raising concerns about its ecological risks. This study investigated the pH-dependent oxidative stress effects of benzophenone-3 (BP-3) on Carassius auratus by measuring four key biomarkers—superoxide dismutase, catalase, glutathione, and malonaldehyde in liver tissue. The results demonstrated that BP-3 exposure led to significant oxidative stress, as evidenced by altered antioxidant levels and increased lipid peroxidation. The integrated biomarker response indicated that stress was more pronounced at higher BP-3 concentrations and varied with pH, being greatest under acidic conditions. These findings demonstrate that the toxicity of BP-3 is strongly influenced by environmental pH, highlighting the necessity of incorporating pH variations into ecological risk assessments of ionizable organic pollutants.
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MICROCHIMICA ACTA
·
2025
·Vol. 193
·期 1
·49
·
IF 5.3
Wang Chuang,Peng Yue,Miao Yuanyuan,Sun Xinyuan,Cheng Wenting,Li Jinlong
DNA methyltransferases (MTases) play a critical role in epigenetic regulation and are closely associated with the occurrence and development of various diseases. To address the limitations of current detection methods, which often suffer from complicated operation and limited sensitivity we have developed a label-free fluorescent biosensing platform based on DNA-templated silver nanoclusters (DNA-AgNCs) for the detection of DNA adenine methyltransferase (Dam MTase) activity and the screening of its inhibitors. In this system, DNA-AgNCs signal probes are synthesized via a simple reduction reaction. Dumbbell-shaped DNA substrates are designed to be specifically methylated by Dam MTase in the presence of S-adenosylmethionine (SAM). The methylated substrates are subsequently recognized and cleaved by DpnI, yielding DNA fragments with 3′-OH termini. These termini are extended by terminal deoxynucleotidyl transferase (TdT) in the presence of dGTP, generating G-rich sequences, which hybridize with the DNA-AgNCs and significantly enhance the fluorescence intensity. This cascade reaction enables the sensitive detection of Dam MTase activity. Compared with the other approaches, the proposed method exhibits a wider linear range (0.5–10 U/mL) and a lower detection limit (0.165 U/mL). Furthermore, inhibition studies demonstrate that 5-fluorouracil and gentamicin effectively suppress Dam MTase activity. The method also shows excellent performance in complex biological samples. Overall, this fluorescence-based strategy offers high sensitivity and excellent specificity, demonstrating broad application prospects in epigenetic research and drug discovery. Graphical
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MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 11
·1-12
·
IF 6.408
Sha Zhou,Wang Chunnan,Ma Rui,Gao Xiaochun,Sun Shuqing
Uniform and monodisperse quantum dot (QD)–encoded magnetic microbeads with Janus structure were produced in a microfluidic device via photopolymerization. UV light through a microscope objective was used to solidify the microbeads which showed sharp interfaces and excellent magnetic responses. QDs with different emission peaks (450 nm for blue and 640 nm for red) were mixed at different ratios to provide three spectral codes. The QD-encoded microbeads can be distinguished by analyzing their fluorescent images in HSV color space. After hydrolysis of the anhydride group in alkaline solution, protein was immobilized on microbeads via activation of carboxyl groups using (1-ethyl-3(3-dimethylaminoprophyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS). A microhole array in polydimethylsiloxane (PDMS) substrates with a specific size was fabricated to trap individual microbeads in a single microhole. The combination of Janus-structured QD-encoded magnetic microbeads and microhole arrays facilitates both flexibility, binding kinetics, sensitivity for suspension assay, and fluorescence mapping analysis for conventional biochips, thus providing a novel platform for multiplex bioanalysis. The capability of this integration for multiplex immunoassays was verified using three kinds of IgG and their corresponding anti-IgG. A detection limit of 0.07 ng/mL was achieved for human IgG, indicating practical applications in various fields. Graphical abstract
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Journal of Orthopaedic Surgery and Research
·
2023
·Vol. 18
·期 1
·1-12
·
IF 2.6
Su Hairong,Yang Yulan,Lv Wanchun,Li Xiaoli,Zhao Binxiu
Background Osteoporosis (OP) is a systemic skeletal disorder with increased bone fragility. Human bone marrow mesenchymal stem cells (hBMSCs) have multi-lineage differentiation ability, which may play important roles in osteoporosis. In this study, we aim to investigate the role of hBMSC-derived miR-382 in osteogenic differentiation.Methods The miRNA and mRNA expressions in peripheral blood monocytes between persons with high or low bone mineral density (BMD) were compared. Then we collected the hBMSC-secreted sEV and examined the dominant components. The over-expression of the miR-382 in MG63 cell and its progression of osteogenic differentiation were investigated by qRT-PCR, western blot and alizarin red staining. The interaction between miR-382 and SLIT2 was confirmed by dual-luciferase assay. The role of SLIT2 was also confirmed through up-regulation in MG63 cell, and the osteogenic differentiation-associated gene and protein were tested.Results According to bioinformatic analysis, a series of differential expressed genes between persons with high or low BMD were compared. After internalization of hBMSC-sEV in MG63 cells, we observed that the ability of osteogenic differentiation was significantly enhanced. Similarly, after up-regulation of miR-382 in MG63 cells, osteogenic differentiation was also promoted. According to the dual-luciferase assay, the targeting function of miR-382 in SLIT2 was demonstrated. Moreover, the benefits of hBMSC-sEV in osteogenesis were abrogated through up-regulation of SLIT2.Conclusion Our study provided evidence that miR-382-contained hBMSC-sEV held great promise in osteogenic differentiation in MG63 cells after internalization by targeting SLIT2, which can be served as molecular targets to develop effective therapy.
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MOLECULAR AND CELLULAR BIOCHEMISTRY
·
2021
·Vol. 476
·期 5
·2061-2073
·
IF 3.396
Wang Gang,Wang Hefeng,Zhang Lijuan,Guo Feng,Wu Xiangyu,Liu Yang
Studies have reported that miR-195-5p plays a role in the Hirschsprung disease (HSCR). Our previous work found GDNF family receptor alpha 4 (GFRA4) is also associated with HSCR. In this study, we focused on whether miR-195-5p induces the absence of enteric neurons and enteric neural crest in HSCR by regulating GFRA4. The expression levels of GFRA4 and miR-195-5p in colon tissues were evaluated by real-time PCR (RT-PCR) assay. We overexpressed GFRA4 or miR-195-5p in SH-SY5Y cells, the cell proliferation, cell cycle, apoptosis and invasion were subsequently investigated by CCK-8 assay, EdU staining, Flow cytometry analysis and Transwell assay, respectively. We also established the xenograft model to detect the effect of miR-195-5p on tumor growth and GFRA4 and p-RET expressions. GFRA4 expression was significantly downregulated in the HSCR colon tissues when compared with that in the control tissues. Overexpression of GFRA4 significantly promoted proliferation, invasion and cell cycle arrest, and inhibited apoptosis of SH-SY5Y cells. We also proved that GFRA4 is a direct target of miR-195-5p, and miR-195-5p inhibited proliferation, invasion, cell cycle arrest and differentiation, and accelerated apoptosis in SH-SY5Y cells which can be reversed by GFRA4 overexpression. Furthermore, we demonstrated that miR-195-5p suppressed tumor growth, and observably decreased GFRA4 and p-RET expressions. Our findings suggest that miR-195-5p plays an important role in the pathogenesis of HSCR. MiR-195-5p inhibited proliferation, invasion and cell cycle arrest, and accelerated apoptosis of nerve cells by targeting GFRA4.
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Scientific Reports
·
2022
·Vol. 12
·期 1
·1-19
·
IF 4.996
Ilhan Recep,Üner Göklem,Yilmaz Sinem,Atalay Sahar Esra,Cayli Sevil,Erzurumlu Yalcin,Gozen Oguz,Ballar Kirmizibayrak Petek
Endoplasmic reticulum-associated degradation (ERAD) is a well-characterized mechanism of protein quality control by removal of misfolded or unfolded proteins. The tight regulation of ERAD is critical for protein homeostasis as well as lipid metabolism. Although the mechanism is complex, all ERAD branches converge on p97/VCP, a key protein in the retrotranslocation step. The multifunctionality of p97/VCP relies on its multiple binding partners, one of which is the endogenous ERAD inhibitor, SVIP (small VCP-interacting protein). As SVIP is a promising target for the regulation of ERAD, we aimed to assess its novel physiological roles. We revealed that SVIP is highly expressed in the rat adrenal gland, especially in the cortex region, at a consistently high level during postnatal development, unlike the gradual increase in expression seen in developing nerves. Steroidogenic stimulators caused a decrease in SVIP mRNA expression and increase in SVIP protein degradation in human adrenocortical H295R cells. Interestingly, silencing of SVIP diminished cortisol secretion along with downregulation of steroidogenic enzymes and proteins involved in cholesterol uptake and cholesterol biosynthesis. A certain degree of SVIP overexpression mainly increased the biosynthesis of cortisol as well as DHEA by enhancing the expression of key steroidogenic proteins, whereas exaggerated overexpression led to apoptosis, phosphorylation of eIF2α, and diminished adrenal steroid hormone biosynthesis. In conclusion, SVIP is a novel regulator of adrenal cortisol and DHEA biosynthesis, suggesting that alterations in SVIP expression levels may be involved in the deregulation of steroidogenic stimulator signaling and abnormal adrenal hormone secretion.
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Human Cell
·
2023
·Vol. 36
·期 3
·987-996
·
IF 4.3
Yiou Wang,Zhihong Wang,Shibai Zhu,Shanni Li,Wenwei Qian
The dysregulation of microRNAs plays a critical role in the development of rheumatoid arthritis (RA). This study aims to explore the functional significance of miR-326 in RA. The RT-qPCR results showed that miR-326 was downregulated in synovial tissues of RA patients and RA fibroblast-like synoviocytes (RA-FLS). We found that miR-326 could target and reduce the expression of inhibitor of DNA binding 1 (Id1). MTT assay and flow cytometry were conducted to explore the biological function of miR-326. Our data revealed that the upregulation of miR-326 suppressed cell proliferation and induced apoptosis in RA-FLS. In collagen-induced arthritis mice, intraarticular injection of lentivirus carrying miR-326 overexpression vectors could reduce the arthritis score and attenuate synovial inflammation and cartilage destruction. We also found that long non-coding RNA-Ewing sarcoma-associated transcript 1 (lncRNA-EWSAT1) was significantly increased in RA synovial tissues and RA-FLS. The RNA immunoprecipitation and RNA pull-down assay indicated that lncRNA-EWSAT1 directly bound and negatively regulated the expression of miR-326. Knockdown of lncRNA-EWSAT1 could upregulate miR-326 expression and attenuate its proliferation inhibition and apoptosis induction effect in RA-FLS. In conclusion, the lncRNA-EWSAT1/miR-326 axis might provide a novel therapeutic target in the treatment of RA.
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