COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2024
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IF 4.9
Zhaolei Zhang,Fengyan Fang,Pingping Zhang,Xiaokang Zhang,Hongchao Ma,Yanhui Wei
Efficient detection of 4- nitrophenol (4-NP) organic pollutant is always a very important issue due to the rapid development of chemical industry and the abuse of pesticides and so on. Here, we designed and synthesized a novel supramolecular fluorescent probe , 4-[4-(acetylamino)styryl]-1-cyclodextrin pyridinium p-toluenesulfonate (ASPT-β-CD), for the selective and sensitive detection of 4-NP. The introduction of β-CD not only improves the water solubility of probe and can also be used as a host to encapsulate 4-NP. In alkaline aqueous solution, the absorption spectrum of 4-NP overlaps the excitation spectrum of ASPT-β-CD and suggests that an internal filtration effect occurs between them. We explored the possible response mechanism and speculated that the high selectivity and high sensitivity were derived from the synergistic effect of inner filtering effect and host-guest interaction. Benefiting from the synergistic effect, the proposed sensor ASPT-β-CD displayed significant "turn-off" after adding 4-NP, while showing a fast response to 4-NP within 2 min and low LOD about 0.01 μM. In addition, this method has been successfully applied to the determination of 4-NP in lake water samples, indicating that the obtained ASPT-β-CD has great practical application potential in the environmental field.
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Journal of Materials Chemistry B
·
2024
·
IF 6.1
Caiyan Yuan,Shuangyan Chang,Chong Zhang,Dirong Dong,Jiahui Ding,Ali Reza Mahdavian,Zheng Hu,Lili Sun,Songwei Tan
Nasopharyngeal carcinoma (NPC) is one of the most common tumors in South China and Southeast Asia and is thought to be associated with Epstein-Barr virus (EBV) infection. Downregulation of latent membrane protein 1 (LMP1) encoded by EBV can reduce the expression of NF-κB and PI3K, induce apoptosis, and inhibit the growth of EBV-related NPC. To targeting cleave Lmp1 oncogene via the CRISPR/Cas9 gene editing system, a post-cross-linked ROS-responsive poly (β-amino ester) (PBAE) polymeric vector was designed for the delivery of CRISPR/Cas9 plasmids both in vitro and in vivo. After composition optimization, the resultant polymer-plasmid polyplex nanoparticles (NPs) showed a diameter of ~ 230 nm and zeta-potential of 22.3 mV with good stability. Compared with the non-cross-linked system, the cross-linked NPs exhibited efficient and quick cell uptake, higher transfection efficiency in EBV-positive C666-1 cells (53.5% vs. 40.6%), more efficient gene editing ability against the Mucin2 model gene (Muc2) (17.9% vs. 15.4%) and Lmp1 (8.5% vs. 5.6%), and lower intracellular reactive oxygen species (ROS) levels. The NPs achieved good tumor penetration and significant tumor growth inhibition in the C666-1 xenograft tumor model via Lmp1 cleavage, indicating their potential for gene therapy of EBV-related NPC.
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Biosensors-Basel
·
2025
·Vol. 15
·期 1
·52
·
IF 5.6
Qi Yuan,Yunqing Wang
High-sensitivity and repeatable detection of hydrophobic molecules through the surface-enhanced Raman scattering (SERS) technique is a tough challenge because of their weak adsorption and non-uniform distribution on SERS substrates. In this research, we present a simple self-assembly protocol for monolayer SERS mediated by 6-deoxy-6-thio-β-cyclodextrin (β-CD-SH). This protocol allows for the rapid assembly of a compact silver nanoparticle (Ag NP) monolayer at the oil/water interface within 40 s, while entrapping analyte molecules within hotspots. The proposed method shows general applicability for detecting hydrophobic molecules, exemplified as Nile blue, Nile red, fluconazole, carbendazim, benz[a]anthracene, and bisphenol A. The detection limits range from 10−6to 10−9M, and the relative standard deviations (RSDs) of signal intensity are less than 10%. Moreover, this method was used to investigate the release behaviors of a hydrophobic pollutant (Nile blue) adsorbed on the nanoplastic surface in the water environment. The results suggest that elevated temperatures, increased salinities, and the coexistence of fulvic acid promote the release of Nile blue. This simple and fast protocol overcomes the difficulties related to hotspot accessibility and detection repeatability for hydrophobic analytes, holding out extensive application prospects in environmental monitoring and chemical analysis.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2026
·
IF 8.7
Keyu Lin,Keyi Liu,Jiangqi Xu,Jiabao Yan,Tonghaoyang Han,Haiyan Gao,Zhen Qin
A V. vinifera GH 17 β-1,3-glucanase was heterologously expressed and characterized. • Its catalytic groove accommodates β-1,6 branches, enabling antifungal action. • VvGlu17a produces β-1,3-oligosaccharides (DP 2–6) with prebiotic potential. • VvGlu17a exhibits potential in agricultural biocontrol and biomedical prebiotic production. β-1,3-Glucanases, a class of pathogenesis-related (PR) proteins, play critical roles in plant defense against phytopathogens. However, the enzymatic mechanism underlying their fungistatic effect remains to be further explored. This study focused on Vitis vinifera GH17 family endo -β-1,3-glucanase (VvGlu17a) to clarify its enzymatic properties and fungistatic mechanism. A genome-wide identification identified 54 GH17 β-1,3-glucanase genes in V. vinifera , with VvGlu17a belonging to subfamily B and possessing only a conserved GH17 domain. The purified recombinant VvGlu17a with optimal activity at pH 6.0 and 55 °C, and a specific activity of 408 U/mg toward linear β-1,3-glucan (curdlan), producing oligosaccharides with degrees of polymerization ranging from 2 to 6. In vitro fungistatic assays demonstrated that VvGlu17a significantly inhibited the growth of four phytopathogens, with the highest inhibition against Colletotrichum gloeosporioides . Structural modeling revealed that VvGlu17a adopts a typical (β/α)₈ TIM-barrel fold with an elongated, twisted catalytic groove containing eight glycosyl-binding sites (−3 to +5). Notably, the unobstructed C6 positions at subsites −1 to +3 allowing the enzyme to accommodate β-1,6-branched glucans—the structural feature of fungal cell wall polysaccharides. This study provides insights into the fungistatic mechanism of GH17 β-1,3-glucanases and their potential for developing biocontrol agents and functional oligosaccharides. Download: Download high-res image (259KB) Download: Download full-size image
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DENTAL MATERIALS
·
2026
·
IF 6.3
Xin Zhou,Zengqi Ye,Xian Tong,Zhaoping Chen,Jiancheng Yu,Yuncang Li,Li Zhu,Cuie Wen,Jixing Lin,Jianfeng Ma
Co-addition of β-spodumene and LM enhances densification of LDGCs. • A-SiO 2 promotes LM-to-LS 2 conversion but increases porosity and defects. • β-spodumene/LM synergy boosts flexural strength and fracture toughness. • Excessive LM or A-SiO 2 reduces density and mechanical performance. • Co-addition of β-spodumene and LM improves manufacturing accuracy. Objectives This study aims to improve the densification and strength of additively manufactured lithium disilicate glass-ceramics (LDGCs) for dental restorations by introducing a β-spodumene–lithium metasilicate (LM)–amorphous silicon dioxide (A-SiO 2 )–glass system. Methods Precursor slurries with varying ratios of β-spodumene, LM, and A-SiO 2 were shaped using digital light processing and subsequently sintered without applied pressure. The resulting LDGC samples were systematically evaluated in terms of density, porosity, phase composition, microstructure, mechanical properties, fracture morphology, and molding accuracy. Results The combined addition of β-spodumene and LM significantly reduced porosity, promoted densification and crystallinity, and produced a uniform interlocked microstructure. In contrast, the introduction of A-SiO 2 facilitated the transformation of LM into lithium disilicate, but increased porosity and defects by impeding densification. The optimized β-spodumene/LM/glass ternary system achieved a maximum flexural strength of ∼332.5 MPa and a fracture toughness of ∼2.3 MPa·m 1/2 , while exhibiting hardness and elastic modulus comparable to natural enamel. Moreover, crown manufacturing demonstrated high molding accuracy. Significance The synergistic addition of β-spodumene and LM effectively enhances densification and strength in additively manufactured LDGCs, establishing a promising material system for high-load aesthetic restorations.
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FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Jiayao Gan,Wenyu Jiang,Wei Liao,Huan Liu,Jielun Hu
The purpose of this research was to investigate the application of azobenzene modified polysaccharide on the single probiotic encapsulation by carbodiimide-mediated amidation and evaluate its targeted delivery potential in colon. Yeast β-glucan (YBG) was first modified by azobenzene groups via esterification (AA-YBG), which was then conjugated to the amino groups on the Escherichia coli Nissle 1917 (EcN) surface membrane through carbodiimide-mediated amidation, resulting in the successful fabrication of single probiotic microcapsules (EcN@AA-YBG). SEM and TEM showed that AA-YBG formed a nano-sacle intact thin film on the surface of EcN through covalent coupling between amino groups on the EcN membrane and the carboxyl groups in AA-YBG molecular chains as evidenced by FT-IR, and the biocompatibility analysis showed that AA-YBG coupled nanolayer has no significant effect on the proliferation of probiotics. In vitro gastrointestinal digestion showed that compared to the free EcN which suffered structural disintegration and lost all the viability within 0.5 h of gastric acid digestion, the EcN@AA-YBG maintained robust structural integrity with viability above 77% after the entire 6 h of digestion. Colonic targeted release analysis revealed that EcN@AA-YBG not only susceptible to enzymatic cleavage by colonic azo reductase and hypoxic colonic condition, triggering the rapid release of probiotics in colon, the adhesion of EcN@AA-YBG to colon mucosa was significantly improved as well. Furthermore, due to the compact and intact surface nanocoating of AA-YBG, the resistance of EcN@AA-YBG to high temperature, antibiotics and reactive oxygen species conditions were all improved significantly.
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ULTRASONICS SONOCHEMISTRY
·
2026
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IF 10.2
Xinran Niu,Di An,Liang Li
This study systematically investigated the effects of ultrasonic pretreatment on the fibrillation behavior of soy protein isolate (SPI), β-conglycinin (7S) and glycinin (11S). A multi-analytical approach combining Thioflavin T fluorescence spectroscopy, SDS-PAGE, dynamic light scattering, zeta potential measurement, and spectroscopic analyses was employed to monitor fibrillation kinetics and structural modifications. Ultrasonication significantly accelerated the fibrillation process by enhancing nucleation, increasing the apparent rate constant of 7S by 44% and that of SPI by 67%. SDS-PAGE analysis indicated that ultrasound treatment promoted protein degradation, generating a greater number of low-molecular-weight peptides, which may have facilitated fibril nucleation. Ultrasound treatment reduced protein particle size, increased surface charge, and promoted conformational transitions toward β-sheet-rich structures, with U-7SN showing a 20.8% rise in β-sheet content. These structural changes were correlated with enhanced functional properties, including improved solubility, emulsifying activity, and viscosity. The findings demonstrated that ultrasound promoted fibrillation by enhancing hydrophobic interactions and the formation of β-sheet structures, offering a theoretical basis for tailoring plant protein nanofibrils with targeted functionalities for food and material applications.
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XENOBIOTICA
·
2026
·
IF 1.5
Jiexin Qiu,Beibei Yao,Qing Yu,Jiajie Wang,Lingling Huang,Jiale Chen,Yufeng Lu,Chunying Wang
Cis-2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside (cis-THSG), a photoisomer of the major bioactive component in Polygoni Multiflori Radix Praeparata (He-Shou-Wu), has enhanced hypolipidemic activity but is associated with idiosyncratic hepatotoxicity. Its metabolic fate under pathological conditions and key clearance enzymes remain unclear.This study aimed to compare metabolite profiles of cis-THSG in normal and hyperlipidemic rats in vivo and in vitro, identify metabolites, and characterise enzyme kinetics in rat liver microsomes (RLMs) and major CYP450 isoforms. Metabolites in plasma, urine, faeces, bile, and intestinal bacteria incubation fluids were analysed by UHPLC-Q-TOF-MS/MS. Enzymatic kinetics and CYP450 isoforms were investigated via chemical inhibition assays.A total of 27 metabolites were characterised; cis-THSG underwent rapid glucoside hydrolysis, and its aglycone underwent phase I and II reactions. Hyperlipidaemia reduced metabolites to 20 and markedly increased plasma cis-THSG and aglycone abundance. Kinetic parameters were Km 32.86 μmol/L, Vmax 0.7285 μmol/(min·mg protein), CLint 0.02217 L/(min·mg protein). Rat liver microsomal CYP isoforms analogous to human CYP3A4 and CYP2C9 mediated cis-THSG oxidative metabolism.This study provides the first comprehensive metabolite map of cis-THSG, identifies its aglycone as a potential bioactive form, and reveals a disease-related metabolic shift. CYP phenotyping offers a mechanistic basis for predicting clinical drug–drug interactions.
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ChemistrySelect
·
2026
·Vol. 11
·期 36
·e74500
·
IF 2.3
Cong Zhu,Tianqi Li,Wenhao Liu,Teng Wang,Mengjing An,Yunjun Luo
Multi-hydrogen bond chain extenders are widely used to improve the mechanical properties of polyurethane elastomers, but their incorporation often requires polar organic solvents, limiting solvent-free processing and large-scale production. Herein, a one-step solvent-free strategy was developed by dispersing β-hydroxyethylurea (2-Hu) in low-molecular-weight polyethylene glycol (PEG) to construct a mixed chain-extending system for hydroxyl-terminated polycaprolactone polyether (HTCE)-based polyurethane. Fourier transform infrared spectroscopy (FTIR) confirmed successful polyurethane formation, while small-angle x-ray scattering (SAXS) and low-field nuclear magnetic resonance (LFNMR) revealed that 2-Hu enhanced hydrogen bonding, promoted hard-domain aggregation, and strengthened microphase separation. Density functional theory (DFT) calculations demonstrated stronger intermolecular hydrogen-bonding interactions for 2-Hu than for 1,4-butanediol (BDO). Consequently, the crosslink density increased from 3.06 × 10 − 4 to 3.41 × 10 − 4 mol·mL − 1 , and the tensile strength improved from 0.86 to 1.00 MPa. Moreover, the 2-Hu-containing polyurethane exhibited complete elastic recovery after 10 loading–unloading cycles, demonstrating excellent reversible deformation enabled by the dynamic hydrogen bonding network. This solvent-free strategy provides a simple and scalable route for preparing polyurethane elastomers containing multi-hydrogen bond interactions. Graphical 2-Hu is dispersed in polyethylene glycol (PEG) and then incorporated into polyurethane systems. The formation of multiple hydrogen bonds creates a dynamic crosslinked network. This structural modification effectively enhances the overall mechanical performance, including improved strength and toughness. The strategy offers a facile route to develop high-strength polyurethane elastomers with tunable properties.
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CHINESE JOURNAL OF CHEMISTRY
·
2026
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IF 5.7
Junpu Yang,Kairui Zhang,Shangwei Guo,Wenxuan Yang,Shuode Feng,Yining Zheng,Guobing Yu,Weinan Sun,Peng Lin,Jian Lin
Comprehensive Summary Radiation scintillators that combine high sensitivity, stability, and multimodal detection capability are highly desirable for advanced radiation imaging and monitoring. Herein, we report a rational lanthanide-doping strategy to construct a high-performance metal–organic framework (MOF) scintillator, namely Eu 3+ ⊂Bi-ndc . In this design, high-atomic-number Bi 3+ nodes serve as efficient X-ray absorbers, while Eu 3+ ions act as isolated luminescent activators, and rigid 2,6-naphthalenedicarboxylate (ndc 2– ) ligands function as effective antennae to facilitate energy transfer and suppress non-radiative relaxation. Under X-ray excitation, Eu 3+ ⊂Bi-ndc shows intense radioluminescence with a low detection limit of 2.5 μGy·s –1 , a light yield of 25,695 photons MeV –1 , and outstanding irradiation stability. Flexible scintillating films fabricated from Eu 3+ ⊂Bi-ndc enable high-resolution X-ray imaging with a spatial resolution of approximately 7 lp·mm –1 . Remarkably, Eu 3+ ⊂Bi-ndc also exhibits measurable β-ray–induced scintillation, representing the first example of a lanthanide-containing MOF functioning as a β-ray scintillator. This study advances a synergistic high- Z /lanthanide MOF paradigm that bridges X-ray and β-ray scintillation, paving the way for novel multifunctional radiation detectors.
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WATER ENVIRONMENT RESEARCH
·
2026
·Vol. 98
·期 4
·e70380
·
IF 2.8
Shifeng Li,Yan Zhou,Shiyu Wang,Shuhua Yao,Min Xiao
Arsenic contamination in water, particularly from highly toxic arsenite (As(III)), continues to pose a significant global public health challenge due to its high mobility and resistance to conventional treatment methods. This study presents an innovative core-shell β-FeOOH@MnO 2 nanocomposite, which synergistically integrates MnO 2 nanosheets to oxidize As(III) into adsorptive arsenate (As(V)) and β-FeOOH to facilitate the efficient uptake of As(V). This approach addresses common limitations such as particle aggregation and suboptimal adsorption observed in pure MnO 2 systems. The nanocomposite, synthesized by hydrothermal methods, exhibits a doubled surface area of 172.95 m 2 /g, offering numerous active sites and mesopores conducive to rapid mass transfer. Under optimized conditions (pH 3.0, 0.4-g/L dosage), the nanocomposite achieved 98.39% removal of As(III) and a maximum adsorption capacity of 50.26 mg/g at 25°C, following pseudo-second-order kinetics and endothermic chemisorption. Characterization techniques, including X-ray diffraction (XRD), transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS), confirmed the synergistic mechanism, wherein MnO 2 oxidizes As(III) through the reduction of Mn 4+ to Mn 3+ , and the resulting As(V) is adsorbed by β-FeOOH. This advancement offers a cost-effective and efficient solution for improved arsenic remediation in water treatment applications. Graphical A core-shell β-FeOOH@MnO 2 nanocomposite effectively removes toxic arsenite by oxidizing mobile As(III) to adsorptive As(V) and adsorbing it, achieving high removal efficiency.
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Journal of Water Process Engineering
·
2026
·
IF 6.6
Yuhang Wang,Xiaoran Zhang,Xin Gao,Qiming Li,Qiuyue Hu,Xingyu Wang,Lincai Peng,Heng Zhang
The development of high-performance biomass adsorbents from renewable resources is crucial to mitigating antibiotic pollution in water. In this study, an eco-friendly composite porous aerogel was fabricated by crosslinking regenerated cellulose (RC) derived from corn stalk parenchyma with carboxylated β-cyclodextrin (βCD-COOH). The composite possesses a hierarchical micro-nano architecture that exposes abundant active sites, enabling remarkable selectivity and efficiency in capturing ciprofloxacin (CIP). Through a dual cross-linking strategy combining physical entanglement and covalent bonding, βCD-COOH was effectively integrated into the RC network, which synergistically enhanced CIP adsorption via hydrophobic cavity inclusion and carboxyl-mediated electrostatic interactions. Attributed to the synergistic effects of host-guest complexation and electrostatic interactions, the composite material exhibited a significantly enhanced adsorption capacity toward CIP, reaching a capacity of 22.97 mg/g. This work offers a sustainable and scalable approach to converting agricultural waste into an efficient adsorbent for antibiotic removal from aqueous systems.
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