Systems Microbiology and Biomanufacturing
·
2025
·
IF 2.9
Shi Yu,Li Huifan,Lu Yuqi,Chen Ming,Yang Hua,Fu Dongmei
A simple and robust reversed-phase high performance liquid chromatography procedure was developed with only an aqueous H 3 PO 4 solution (pH 2.1) as the mobile phase for the simultaneous determination of nine organic acids (oxalic, formic, malic, lactic, acetic, citric, succinic, fumaric, and gallic acids) in beer based on a polar-copolymerized 3-chloropropyl C18 column. The proposed method was validated according to linearity, limits of detection, accuracy, precision, repeatability, and reproducibility. Quantifications of the nine organic acids in 12 commercial Chinese beer samples from five different brands were conducted only after degassing and filtration of the samples. In all beer samples, the most abundant organic acid was succinic acid; its concentration was in the range of 907.99–3557.22 mg/L. This was followed by malic acid (170.39–936.78 mg/L). Principal component analysis was used to compare the different brand of beers according to organic acid concentration, and results may provide a reference for the evaluation of beer quality and taste.
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 9
·1-8
·
IF 5.479
Fang Dandan,Zhang Shupei,Dai Hong,Li Xiuhua,Hong Zhensheng,Lin Yanyu
An ultrasensitive competitive-type electrochemiluminescence immunoassay for the mycotoxin zearalenone is described. The method is based on the use of (a) a mimotope peptide that was selected from a phage displayed peptide library and used to substitute ZEN for designing the competitive assay; (b) NiFe 2 O 4 nanotubes with large specific surface area loaded with the ECL probe Ru(bpy) 3 2+ ; and (c) poly(vinylpyrrolidone) (PVP)-assisted synthesis of TiO 2 mesocrystals that acts as the sensing platform and support for antibody immobilization. Under the optimized conditions and at an ECL working potential of 1.1 V, a linear response is found for ZEN in the 0.1 to 1.0 × 10 −5 ng·mL −1 concentration range with a detection limit as low as 3.3 fg·mL −1 .
An ultrasensitive competitive-type electrochemiluminescence (ECL) immunosensor based on mimotope peptide was constructed for the detection of Zearalenone.
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JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2025
·Vol. 172
·期 4
·047502
·
IF 3.3
Lanyue Wang,Beibei Wang,Kai Kang,Xueping Ji,Zhenzhen Zhao,Congwei Li,Yuping Wang
An electrochemical sensor utilizing Au-Ni@ etched metal-organic framework (etched MOF) on a glassy carbon electrode (GCE) was constructed for ultrasensitive detection of 3-nitrophenol (3-NP). The Au-Ni@etched MOF in situ nanocomposite was synthesized via a simple one-step process that combines the electrochemical etching of Ni-hemin MOF with the simultaneous electrodeposition of Au-Ni bimetallic nanoparticles. The etched MOF not only provides abundant active sites for adsorbing 3-NP, but also serves as a supporter for loading Au-Ni bimetallic nanoparticles. This innovative design endows the electrochemical sensor with exceptional electrocatalytic activity towards 3-NP, achieving a low detection limit of 0.015 μM over the ranges of 0.06 ∼ 10 μM and 10 ∼ 200 μM. Moreover, the electrochemical sensor also shows excellent sensitivity, repeatability, and stability in 3-NP detection. This method offers a highly sensitive electrochemical strategy for accurately assaying 3-NP in water samples and opens new avenues for the application of MOF derivatives in electrochemical platforms.
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BIORESOURCE TECHNOLOGY
·
2015
·
IF 4.494
Yu-Cai He,Dan-Ping Zhang,Zhi-Cheng Tao,Yun Lu,Yun Ding,Feng Liu,Zheng-Zhong Zhu,Huan Rui,Gao-Wei Zheng,Xian Zhang
To reduce dependence on the expensive cofactor and effectively biotransform ethyl 4-chloro-3-oxobutanoate, l -glutamine and glycine were found to enhance the content of intracellular NADH and the reductase activity. Adding the mixture of 200 mM of l -glutamine and 500 mM of glycine to the reaction media, a 1.67-fold of reductase activity was increased over the control without the addition of the two compounds. Moreover, β-cyclodextrin (0.4 mol β-cyclodextrin/mol ethyl 4-chloro-3-oxobutanoate) was also added into this reaction media, and the biocatalytic activity of the whole-cell biocatalyst of Escherichia coli CCZU-K14 was increased by 1.34-fold than that without β-cyclodextrin. In this β-cyclodextrin–water media containing l -glutamine (200 mM) plus glycine (500 mM), ethyl ( S )-4-chloro-3-hydroxybutanoate (>99% ee ) could be obtained from 3000 mM ethyl 4-chloro-3-oxobutanoate in the yield of 98.0% after 8 h. All the positive features demonstrate the potential applicability of the bioprocess for the large-scale production of ethyl ( S )-4-chloro-3-hydroxybutanoate.
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JOURNAL OF THE EUROPEAN CERAMIC SOCIETY
·
2025
·
IF 6.2
Hao Li,Fei Liu,Lijin Cheng,Jiarui Huang,Shaojun Liu
Complex perovskite-structured Ba(Zn 1/3 Nb 2/3 )O 3 (BZN) ceramics with high B-site 1:2 ordering degree and excellent dielectric properties were successfully fabricated using digital light processing (DLP). BZN ceramic slurry with 82 wt. % solid loading, low viscosity, and high curing properties was used to prepare green bodies. The samples were exposed to debinding process in vacuum and then sintered at 1350–1450 ℃ for 4 h. The as-sintered BZN ceramics with disordered B-site structure exhibited significantly low dielectric properties. However, the BZN ceramics annealed at 1300 ℃ for 24 h with high B-site 1:2 ordering degree exhibited excellent dielectric properties ( ε r = 38.9, Q × f = 89,600 GHz, and τ f = 22 ppm/℃) that were identical to those of dry-pressed ceramics. Furthermore, a DLP-fabricated cylindrical BZN dielectric resonator antenna exhibited a >90% radiation efficiency and resonant frequency of 7.10 GHz ( S 11 = -29.94 dB), which is consistent with the simulation results.
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International Journal of Biological Sciences
·
2017
·
IF 3.873
Chenxiao Yu,Yang Jiao,Jiao Xue,Qi Zhang,Hongying Yang,Ligang Xing,Guangxia Chen,Jinchang Wu,Shuyu Zhang,Wei Zhu,Jianping Cao
Non-small cell lung cancer (NSCLC) is the most common type of lung cancer. (-)-Epigallocatechin-3-gallate (EGCG), a major polyphenol in green tea, is widely studied as a cancer chemopreventive agent with potential anti-cancer effects. The NF-E2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling pathway is considered to mediate cellular resistance to EGCG. Metformin, a classical antidiabetic drug, has been shown to prevent cancer progression. Researchers have not reported whether metformin potentiates the anti-cancer efficacy of EGCG. In this study, metformin inhibited HO-1 expression and augmented the anti-tumor effect of EGCG. Metformin also enhanced ROS (reactive oxygen species) generation induced by EGCG (100 μM), subsequently resulting in apoptosis. Based on the results of the in vivo study, size of xenografts treated with the combination of metformin and EGCG was smaller than other groups. Mechanistically, metformin modulated the EGCG-activated Nrf2/HO-1 pathway through Sirtuin 1 (SIRT1)-dependent deacetylation of Nrf2. Moreover, metformin upregulated SIRT1 expression partially through the NF-kB pathway. Comparatively, the combination of EGCG and metformin showed little impact on normal lung epithelial BEAS-2B cells. Based on our findings, metformin sensitized NSCLC cells to the EGCG treatment by suppressing the Nrf2/HO-1 signaling pathway.
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MEDICAL SCIENCE MONITOR
·
2018
·
IF 1.894
Xuechan Tang,Xiaoli Xie,Xin Wang,Yan Wang,Xiaoyu Jiang,Huiqing Jiang
Background Piwi-interacting RNA (piRNA) is the largest class of small non-coding RNA, which has also been identified in somatic tissues, and aberrant expression of piRNAs in tumor tissues may be implicated in carcinogenesis. piR-823 is increased in liver cirrhosis and hepatocellular carcinoma (HCC). However, there is no report on the function of piR-823 in hepatic stellate cells (HSCs) activation during hepatic fibrosis. The present study investigated the role of piR-823 in HSC activation. Matreial/Methods Liver fibrosis was induced in mice by carbon tetrachloride (CCL 4 ) injection and bile duct ligation (BDL). The primary HSCs were isolated from mice and cultured. The expression of piR-823 was measured by real-time PCR. The effect of piR-823 on HSCs was evaluated by either sense sequence or antisense sequence of piR-823 carried by liposome. Proteins binding to piR-823 were assayed by RNA pull-down technique and liquid chromatography-mass spectrometry (LC-MS). Results Our data for the first time show that piR-823 is significantly upregulated in activated HSCs. Overexpression of piR-823 promoted HSC proliferation, α-SMA and COL1a1 production, whereas inhibition of piR-823 suppressed the activity of HSCs. Interestingly, the combination of piR-823 and EIF3B promoted TGF-β1 expression. Conclusions Our data illustrate a novel mechanism of piR-823 in HSC activities. The combination of piR-823 and EIF3B increased TGF-β1 expression, which activates HSCs in liver fibrosis. piR-823 may be a new target in the treatment of liver fibrosis.
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Food & Function
·
2022
·Vol. 13
·期 8
·4612-4623
·
IF 6.317
Xiang Ye,Wen Chen,Pengcheng Tu,Ruoyi Jia,Yangyang Liu,Qiong Tang,Chuan Chen,Caihong Yang,Xiaodong Zheng,Qiang Chu
Cyanidin-3-O-glucoside (C3G), an essential representative of anthocyanins, has been proved to possess a myriad of biological activities. However, the effects of C3G on glucose metabolism and its underlying molecular mechanisms remain elusive. The aim of the present study was to investigate the metabolic impact of C3G on db/db mice and to determine whether its consequent anti-diabetic effects were related to glucose transporter-1 (GLUT-1) by in vivo and in vitro studies. As a result, through diabetic db/db mice, C3G treatment was found to significantly reduce the fasting blood glucose level and increase glycogen synthesis, which were associated with upregulation of GLUT-1 expression in the liver of the mice. In addition, in liver cells of the HepG2 and L02 lines, we further discovered that C3G could effectively promote glucose consumption by regulating the Wnt/β-catenin-WISP1 signaling pathway. Nevertheless, such effects would be restricted when the expression of GLUT-1 was blocked by the inhibitor IWR-1. Meanwhile, molecular docking technology was applied to simulate the possible action sites of C3G at the molecular level, and the results indicated that C3G might bind to β-catenin. In conclusion, our study provided evidence of the antihyperglycemic effect of C3G in vivo and in vitro via regulating GLUT-1 expression and the related signaling pathways.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
·
IF 5.4
Xiaoqi Ban,Jin Yao,Wanzhong Yin,Weifan Du,Taozhong Zhang,Wenju Sun,Yulian Wang
The depletion of high-grade sphalerite has directed attention to smithsonite as a strategic substitute for sustaining zinc supply. However, its close association with quartz complicates selective separation, while conventional direct flotation requires moderately to strongly alkaline pulps, causing equipment corrosion, excessive reagent consumption, and unstable concentrate quality that hinder large-scale use. To address this issue, 1-dodecyl-3-methylimidazolium bromide (DMB) was employed as a new collector, providing an alternative chemical environment and enabling, for the first time, an efficient reverse-flotation route for separating smithsonite from quartz. Micro-flotation tests showed that at pH = 7.0 with only 30 mg/L DMB, the concentrate reached 47.05 % Zn with 92.78 % Zn recovery and 95.03 % desilication efficiency, demonstrating both high selectivity and low reagent consumption. Electrokinetic, spectroscopic, and microscopic analyses revealed that DMB adsorption on quartz is governed by electrostatic attraction and hydrogen bonding, increasing surface potential, hydrophobicity, and roughness, whereas electrostatic repulsion limited adsorption on smithsonite, preserving its hydrophilicity. This study establishes a simplified flotation route independent of strong alkalinity, showing high adaptability to high-silica, low-grade smithsonite ores and offering valuable theoretical insight and a broader framework for advancing efficient recovery and utilization of oxidized zinc resources.
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CONSTRUCTION AND BUILDING MATERIALS
·
2025
·
IF 8
Kai Ma,Xingyu Gan,Weichao Li,Laibo Li,Lingchao Lu,Haiming Zhang
Carbon sequestration of cement-based composites is an essential pathway towards the strategic goal of achieving carbon neutrality. However, the efficiency of cement-based composites to sequester carbon dioxide (CO 2 ) is limited. Inspired by legume structure, a sandwich-structured graphene oxide embedded with In(OH) 3 (SGOI) was synthesized to simultaneously enhance the CO 2 sequestration capacity, hydration kinetics, and mechanical performances of cement-based composites. The effect of SGOI content on the CO 2 sequestration capacity, hydration kinetics, and mechanical performances of cement-based composites was evaluated. The results show that the CO 2 sequestration, compressive strength, flexural strength, and eco-mechanical index of the hardened cement paste containing 0.03 wt% SGOI were achieved 52.55 g/kg, 105.1 MPa, 13.9 MPa, and 0.75 kgCO 2 /MPa, representing enhancements of 255.07 %, 32.70 %, 34.95 %, and 167.86 % compared to those of cement paste without SGOI, respectively. Meanwhile, the hydration reaction rate and hydration degree of cement at each stage were improved. The enhancement of these parameters is derived from the structural characteristics of SGOI, including the specific surface area (30.52 m 2 /g), interlayer distance (14.85 nm), and roughness (14.10 nm). One ton of hardened cement paste containing 0.03 wt% SGOI can sequestrate at least 31.09 kg of CO 2 within 28 days, highlighting its significant potential for promoting CO 2 neutrality.
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Journal of Materials Chemistry A
·
2025
·
IF 9.5
Guangsen Tian,Shouzheng Li,Shaojia Song,Hongxi Zhao,Linfeng Zhang,Huadong Wu,Ye Luo,Jianding Li,Jia Guo,Qun Yi
The rational design of D–A type polymeric photocatalysts using triphenylamine (TPA) as the electron donor (D) and a benzothiadiazole derivative (BTDO) as the electron acceptor (A) is investigated. To optimize photocatalytic hydrogen evolution, we systematically enhanced the electron-donating capability by incorporating oligothiophene linkers of different lengths, monothiophene (T), bithiophene (DT), and terthiophene (TT), between the TPA and BTDO units. Comprehensive characterization revealed that the number of thiophene rings significantly affects both the photophysical and electrochemical properties of the polymers, thereby directly affecting photocatalytic activity. The results demonstrate that introducing thiophene oligomers significantly improves performance, with the terthiophene (TT) linker proving exceptionally advantageous. The TPA-TT-BTDO polymer exhibits a substantially broadened visible light absorption range, a reduced bandgap, and most critically, highly efficient suppression of photogenerated charge carrier recombination. This enhancement is attributed to the unique coplanar conformation enforced by the TT unit, which minimizes the dihedral angle between TPA and BTDO, facilitating intramolecular charge transfer. TPA-TT-BTDO achieved an outstanding photocatalytic hydrogen evolution rate of 101.2 mmol h−1 g−1 under visible light (λ ≥ 420 nm) at 10 °C without noble metal co-catalysts. Remarkably, its activity further increased to 125 mmol h−1 g−1 under natural sunlight irradiation. Systematic analysis revealed that longer thiophene chains induce a bathochromic shift, diminish PL intensity, yet improve photoelectrochemical properties and charge transfer efficiency. This work validates the molecular design strategy of precisely controlling oligothiophene chain length to modulate the band structure and electronic properties of D–A polymers. The superior performance of TPA-TT-BTDO stems synergistically from its extended light harvesting, efficient charge separation, and rapid interfacial transfer. These findings may provide crucial insights and practical guidance for developing high-efficiency polymeric photocatalysts through targeted molecular engineering.
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Nanoscale
·
2025
·Vol. 17
·期 35
·20465-20475
·
IF 5.1
Weiyi Li,Min Zhang,Shen Cai,Yan Xin,Huajun Tian
Alluaudite-type Na2+2xFe2−x(SO4)3 has been a promising cathode material for sodium-ion batteries (SIBs) due to its high operating voltage and stable structure. However, its actual electrochemical performance suffers from intrinsic sluggish kinetics and poor electronic conductivity. In this work, for the first time, we propose a Na2.48(Fe0.89Mg0.03Sn0.04)1.76(SO4)3 cathode material prepared via a Mg/Sn co-doping strategy. Inactive Mg2+ stabilizes the structure, while Sn4+ inhibits the decomposition of electrolytes under high voltage. The Mg/Sn co-doping strategy enhances the kinetics of sodium ion diffusion reactions, leading to improved electrochemical properties, especially at low temperatures. The optimal NFMS/C-Sn0.03 cathode exhibits a long-term capacity retention of 91.6% after 1500 cycles at 5C and outstanding reversible capacities of 74.3 and 58.3 mAh g−1 at 10C and even at 50C, respectively. Furthermore, the NFMS/C-Sn0.03 cathode demonstrates a high capacity retention of 95.5% at −5 °C and 88.4% at −15 °C, with a remarkable capacity retention of 93.9% after 1000 cycles at room temperature and 85.5% after 700 cycles at −15 °C, respectively. Electron paramagnetic resonance (EPR) and atomic force microscopy (AFM) techniques confirmed that the presence of unpaired electrons and enhanced electronic conductivity could be attributed to the Mg/Sn co-doping. This work provides a feasible approach for designing low-cost, durable, low-temperature, and high-performance cathode materials for SIBs.
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