JOURNAL OF NANOBIOTECHNOLOGY
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2021
·Vol. 19
·期 1
·1-17
·
IF 10.435
Pan Xiang,Qi Yuchen,Du Zhen,He Jian,Yao Sheng,Lu Wei,Ding Kefeng,Zhou Min
Background Colorectal cancer is a common malignancy occurring in the digestive system and ranks second in cancer mortality worldwide. In colorectal cancer, hydrogen sulfide (H2S) is selectively upregulated, resulting in the further exacerbation of the disease. Therefore, the clearance of H2S and the regulation of the enzymes on the H2S pathways are of great significance for colorectal cancer therapy.Methods Here, we investigated the H2S content in various clinical tumor tissues from patients and confirmed that overproduced concentration of H2S in colorectal cancer. Accordingly, we developed an H2S-responsive nanoplatform based on zinc oxide coated virus-like silica nanoparticles (VZnO) for the therapy of colorectal cancer.Results Owing to its excellent H2S scavenging ability, VZnO could effectively reduce H2S content in colorectal cancer to prohibit the growth of CT26 and HCT116 colorectal cancer cells. Moreover, the removal of H2S in colorectal cancer also leads to tumor inhibition through activating ferroptosis, a non-apoptotic form of cell death. The biosafety-related toxicological and pathological analysis confirmed the low toxicity and high safety of VZnO in colorectal cancer treatment. Furthermore, as an H2S-responsible nanosystem, VZnO appears to have no therapeutic effect on other non H2S rich cancers, such as the 4T1 breast cancer model.Conclusion sWe anticipate that the H2S-depletion-induced ferroptosis strategy using zinc oxide-based nanomaterials would provide insights in designing nanomedicines for colorectal cancer-target theranostics and may offer clinical promise.Graphic abstract
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Nature Communications
·
2025
·
IF 15.7
Xiao-Rui Ren,Baichuan Kou,Qing Hao,Francesco Bertocchi,Ying Xu,Lu Wang,Zhen-Lian Zhao,Ting Chen,Li-Jun Wan,Dong Wang
Engineering symmetry breaking is a fertile ground to develop innovative materials with fascinating properties. Herein, we introduce crystallographically incompatible rotation element into two-dimensional polymers and construct twist-stacked covalent organic framework nanofibers (abbreviated as t -COFs). These t -COFs are derived from polymerization between 2,4,6-triformylphloroglucinol and diamines modulated by monodentate alkylamine. Comprehensive electron microscopy analysis reveals their well-defined fibrous morphology and twist-stacking details. The time-dependent growth process study discloses a new radial growth pattern, which enables t -COFs to inherit twist-stacking from hexagonal macrocycle nanotube intermediate. Intriguingly, the twist-stacking mode is found to be intrinsic when using amine modulator, and enantiomeric bias towards one stacking direction can be achieved using chiral amine. Disrupting the inversion symmetry, the consecutive regular rotation of COF layers endows COF nanofibers with structural chirality and large chiroptical response. Twisted two-dimensional materials are materials with fascinating properties. Here, the authors report on the incorporation of a crystallographically incompatible rotation element into two dimensional polymers to construct twist-stacked covalent organic framework nanofibers with interesting chiroptical properties.
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JOURNAL OF MATERIALS SCIENCE
·
2018
·Vol. 54
·期 3
·2535-2551
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IF 2.993
Shen Hao,Song Jiayi,Yang Ye,Su Ping,Yang Yi
The exploration of new tactics for manufacturing artificial immobilized multi-enzyme systems based on enzyme cascades has recently attracted considerable interest because of the urgent need for multi-enzyme catalysts and the high cost of free enzymes. Because of the inevitable limitations of native enzymes such as instability and storage issues, the development of nanozyme–enzyme cascades is needed. A versatile strategy was developed for fabricating an efficient multi-catalyst system by immobilizing glucose oxidase (GO x ) on ferriferous oxide nanocomposites functionalized with nitrogen-doped graphene quantum dots (Fe 3 O 4 @N-GQDs) through DNA-directed immobilization. The Fe 3 O 4 @N-GQDs acted as a carrier for the natural enzyme and showed high peroxidase activity which enabled an enzyme cascade that included GO x to be set up. This multi-catalyst system showed great catalytic activity, reversibility and operational stability. The surfaces of GO x -targeted magnetic nanoparticles were regenerated by mild dehybridization of DNA. The Michaelis constant ( K m ) and maximum initial velocity ( V max ) of the multi-catalyst system were 1.069 mM and 11.2 × 10 −8 M s −1 , respectively, which are considerably better than the corresponding values for adsorbed and free bienzyme combinations. The increased bioactivity of the multi-catalyst system is ascribed to the satisfactory peroxidase-like activity of Fe 3 O 4 @N-GQDs, the enzyme–promoting effect of the QDs and enhancement by DNA-directed immobilization. Because of the diverse range of possible nanozyme–enzyme combinations and high efficiency of this approach, this work provides a novel pathway for the manufacturing of synthetic enzyme catalyst systems, which have great potential in the field of biotechnology.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 412
·期 4
·915-922
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IF 3.637
Wu Zhi-Wei,Xie Xing-Chen,Guo Hong-Ran,Xia Huan,Huang Ke-Jing
A tetrahedral DNA probe can effectively overcome the steric effects of a single-stranded probe to obtain well-controlled density and minimize nonspecific adsorption. Herein, a highly sensitive electrochemical biosensor is fabricated for determination of protein using a tetrahedral DNA probe and rolling circle amplification (RCA). N- and P-co-doped graphene (NP-rGO) is prepared, and AuNPs are then electrodeposited on it for DNA probe immobilization. Benefitting from the synergistic effects of the excellent electrical conductivity of NP-rGO, the stability of the tetrahedral DNA probe and the signal amplification of RCA, the biosensor achieves a low limit of 3.53 × 10 −14 M for thrombin and a wide linear range from 1 × 10 −13 to 1 × 10 −7 M. This study provides a sensitive and effective method for the detection of protein in peripheral biofluids, and paves the way for future clinical diagnostics and treatment of disease.
Graphical abstract
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JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2014
·Vol. 18
·期 10
·2829-2835
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IF 2.234
Wang Mo,Yin Huanshun,Fu Zhengliang,Guo Yunlong,Wang Xinxu,Zhou Yunlei,Ai Shiyun
MicroRNAs (miRNAs), a class of small endogenous nonprotein-coding RNAs, regulate a wide range of biological processes, and their abnormal expressions are related to the growth and development of plants. Thus, a simple, rapid, and highly sensitive assay for miRNA detection is of great significance. In this work, a label-free and ultrasensitive assay for miRNA detection using protein cage nanoparticles has been developed. Apoferritin-encapsulated Cu nanoparticles (Cu-apoferritin) could be immobilized on the electrode through special reaction between amino and carboxyl. Hybridization event between the probe DNA and the target miRNA-159a is confirmed by electrochemical oxidation signal after Cu released into the detection buffer by adjusting the pH. This assay is highly selective and sensitive with a low detection limit of 3.5 fM. Moreover, the developed method can even discriminate single-base mismatched strand between the complementary targets. The effect of abscisic acid on the expression level of miRNA-159a in Arabidopsis thaliana seeds was also investigated.
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MICROCHIMICA ACTA
·
2013
·Vol. 180
·期 7
·573-580
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IF 3.434
Chen Lijian,Wu Na,Sun Bing,Su Haichao,Ai Shiyun
We describe a rapid and convenient colorimetric method for the detection of oxidative DNA damage caused by peroxynitrite (ONOO − ) using unmodified gold nanoparticles (AuNPs). AuNPs are stable in the presence of single-stranded DNA (ssDNA) against the aggregation induced by a high ionic strength. If adsorbed ssDNA are cleaved by ONOO − to form smaller fragments, the AuNPs rapidly aggregate due to electrostatic attraction. As a result, the color of the solution changes from red to blue, and this can be seen with bare eyes. We also have evaluated the activity of the antioxidants gallic acid, ascorbic acid and caffeic acid to scavenge ONOO − . This method therefore also can be applied to screen for anti-oxidation drugs and agents.
ONOO − -induced ssDNA cleavage can be visually detected by a red-to-blue color change of AuNPs.
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MICROCHIMICA ACTA
·
2016
·Vol. 184
·期 2
·541-546
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IF 4.831
Zhang Ruoxi,Deng Li,Zhu Paijin,Xu Shuxia,Huang Chengpeng,Zeng Ying,Ni Shijun,Zhang Xinfeng
The article describes a bienzyme visual system for aptamer-based assay of Hg(II) at nanomolar levels. The detection scheme is based on the finding that Hg(II) ions captured by aptamer-functionalized magnetic beads are capable of inhibiting the enzymatic activity of uricase and thus affect the formation of H 2 O 2 and the blue product, i.e., oxidized tetramethylbenzidine. This strategy allows for a visual detection of Hg(II) at nanomolar levels without additional amplification procedure. Measuring the absorbance at 650 nm, the logarithmic calibration plot is linear in the concentration range of 0.5–50 nM and the limit of detection (LOD) is 0.15 nM. This is as low as the LOD obtained by atomic fluorescence spectrometry (AFS). The ions K + , Mg 2+ , Na + , Ca 2+ , Cu 2+ , Zn 2+ , Fe 3+ , Al 3+ , Co 2+ , AsO 2
− , Ni 2+ , Cd 2+ and Pb 2+ do not have a significant effect on color formation. The method was applied to the analysis of (spiked) river water, lake water, mineral water, tap water and certified reference water samples, and the results agreed well with those obtained by AFS or certified values, with recoveries ranging from 97% to 109%. The relative standard deviation for five parallel detections at a 10 nM Hg(II) level is 5.2%.
A bienzyme-based visual aptasensor was fabricated for label-free detection of nanomolar Hg 2+ in water samples without any amplification or enrichment procedure.
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 8
·1-9
·
IF 5.479
Liu Hongying,Zhu Langlang,Ma Huan,Wen Jiajun,Xu Hanxiao,Qiu Yubing,Zhang Linan,Li Lihua,Gu Chunchuan
The authors describe the preparation of Cu(II)-coated Fe 3 O 4 ) nanoparticles (NPs) that possess excellent peroxidase-like activity. The NPs were formed by chelation between Cu(II) ions and the oxygen functional groups of sodium ligninsulfonate. The morphology and structure of the NPs were characterized by scanning electron microscopy, transmission electron microscopy, X-ray powder diffraction, X-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy. The NPs have an average diameter of 220 nm. They are shown to be viable peroxidase mimics that can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine by hydrogen peroxide to produce a blue coloration. The findings were used to design a colorimetric assay that has a linear response in the 2.5 to 100 μM H 2 O 2 concentration range and a 0.2 μM detection limit. The assay excels by its selectivity, high sensitivity, good selectivity, portability and cost efficiency.
Fe 3 O 4 -Cu 2+ nanoparticles with excellent peroxidase-like activity were successfully prepared via a facile strategy, and then used to design a facile as well as sensitive colorimetric H 2 O 2 sensor. The linear range and the detection limit were 2.5~100 μM and 0.212 μM.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2018
·Vol. 411
·期 19
·4569-4576
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IF 3.307
Wang Kun,Zhai Fu-Heng,He Meng-Qi,Wang Jin,Yu Yong-Liang,He Rong-Huan
A simple fluorescence biosensor is developed based on the enzyme-assisted cascade amplification strategy. The amplification system consists of a hairpin-structure DNA (H-DNA) and exonuclease III. The target DNA can hybridize with the H-DNA and initiate exonuclease III-assisted target recycling amplification to generate abundant G-rich DNA (G-DNA). One region of G-DNA is designed to possess the same sequence as target DNA. Thus, the G-DNA can also hybridize with H-DNA and initiate the digestion of H-DNA. The cascade strategy in this amplification system causes the concentration of G-DNA to grow exponentially. The fluorescence intensity of N -methylmesoporphyrin IX (NMM) is highly enhanced due to the formation of G-quadruplex configuration. Under optimal conditions, the cascade system could achieve an admirable sensitivity with a detection limit of 52 fM for HIV DNA, and guarantees a satisfactory specificity. Moreover, the cascade system could be implemented for other target DNA detections by substituting the recognition region of the H-DNA. In this way, a detection limit of 65 fM for HBV DNA could be achieved by the cascade system. The target DNA analysis in a real serum sample further indicates that this biosensor has potential for future application in clinical diagnosis.
A simple and label-free cascade amplification strategy is developed by exploiting hairpin DNA and EXO III for sensitive DNA detection.
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CHINESE JOURNAL OF POLYMER SCIENCE
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2025
·
IF 4
Yan Qin-Yu,Song Shun-Gang,Zhou Bu-Jie,Hu Jing,Feng Lian-Fang,Gu Xue-Ping,Zhang Cai-Liang
Poly(phenylene oxide) (PPO) exhibits excellent dielectric properties, making it an ideal substrate for high-frequency, high-speed copper-clad laminates. The phenolic hydroxyl group at the end of PPO plays a key role in its reactivity. Accurately quantifying the phenolic hydroxyl content in PPO is essential but challenging. In this study, we proposed a method for measuring the phenolic hydroxyl content of PPO using differential UV absorption spectroscopy. In alkaline solutions, the phenolic hydroxyl in PPO completely ionizes to form phenoxide ions, leading to a significant increase in UV absorbance at approximately 250 and 300 nm. Notably, the differential UV absorbance at approximately 300 nm was directly proportional to the phenolic hydroxyl concentration. Using 2,6-dimethylphenol as a standard, a calibration curve was established to relate the phenolic hydroxyl concentration to differential UV absorbance at approximately 300 nm, providing a precise and straightforward method for phenolic hydroxyl quantification in PPO with distinct advantages over conventional techniques.
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APOPTOSIS
·
2021
·Vol. 26
·期 1
·111-131
·
IF 4.677
Li Na,Wang JuYuan,Zang XiaoLing,Wang ZhaoYang,Zhang Tao,Zhao BaoXiang,Miao JunYing,Lin ZhaoMin
H 2 S is actual an endogenous signaling gas molecule and involved in a range of cell physiological processes. However, the mechanism of endogenous H 2 S regulating autophagy and apoptosis has not been thoroughly investigated. Here, we try to address this issue by using a H 2 S probe, (E)-2-(4-(4-(7-(diethylamino)-2-oxo-2H-chromene-3-carbonyl)-piperazin-1-yl)-styryl)-1, 3, 3-trimethyl-3H-indol-1-ium iodide (CPC), which could react with endogenous H 2 S. Herein, we reported that CPC inhibited autophagy and decreased the expression and activity of NF-E2-related factor 2 (Nrf2), then induced cell apoptosis. CPC inhibited autophagy and promoted apoptosis by inhibiting Nrf2 activation, which was H 2 S dependent. Furthermore, we found that CPC inhibited Nrf2 nucleus translocation by inhibiting glutathionylation of Kelch-like ECH-associated protein 1 (Keap1) at the Cys434 residue. CPC also inhibited various cancer cell growth, but had no effect on normal cell growth in vitro, and inhibited A549 cancer growth, but did not affect normal angiogenesis in vivo . Therefore, we not only found a new inhibitor of autophagy and Nrf2, but also suggested a novel mechanism that endogenous H 2 S could regulate autophagy, apoptosis and Nrf2 activity through regulating glutathionylation of Keap1 at the Cys434 residue.
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BMC Pharmacology & Toxicology
·
2022
·Vol. 23
·期 1
·1-14
·
IF 2.605
Chen Weisin,Yasen Miersalijiang,Wang Hanquan,Zhuang Chenyang,Wang Zixiang,Lu Shunyi,Jiang Libo,Lin Hong
Background Intervertebral disc degeneration results from a variety of etiologies, including inflammation and aging. Degenerated intervertebral discs feature down-regulated extracellular matrix synthesis, resulting in losing their ability to retain water and absorb compression. Celecoxib is a well-known selective cyclooxygenase-2 inhibitor for treating arthritis and relieving pain. Nevertheless, the mechanism of Celecoxib for treating inflammation-related intervertebral disc degeneration has not yet been clarified.MethodProtein synthesis was analyzed by western blot. Fluorescent probes DCFH-DA and MitoSox Red detected reactive oxygen species and were measured by flow cytometry. The activity of the kinase pathway was evaluated by protein phosphorylation. Autophagy was monitored by mRFP-GFP-LC3 transfection and LC3 analysis. Mitochondrial apoptotic proteins were analyzed by western blot and cell membrane integrity was measured by flow cytometry. The autophagic gene was silenced by siRNA.Results In this study, interleukin-1β stimulation reduced the synthesis of aggrecan, type I and II collagen and caused excessive production of reactive oxygen species. We looked for a therapeutic window of Celecoxib for nucleus pulposus cells to regain extracellular matrix synthesis and reduce oxidative stress. To look into nucleus pulposus cells in response to stimuli, enhancement of autophagy was achieved by Celecoxib, confirmed by mRFP-GFP-LC3 transfection and LC3 analysis. The mammalian target of rapamycin and a panel of downstream proteins responded to Celecoxib and propelled autophagy machinery to stabilize homeostasis. Ultimately, inhibition of autophagy by silencing autophagy protein 5 disrupted the protective effects of Celecoxib, culminating in apoptosis.Conclusion In summary, we have demonstrated a new use for the old drug Celecoxib that treats intervertebral disc degeneration by enhancing autophagy in nucleus pulposus cells and opening a door for treating other degenerative diseases.
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