MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 6
·1-10
·
IF 5.3
Liu Hao,Sun Xuyuan,Dai Zhengyuan,Wang Ying,Li Li,Fan Jie,Ding Yaping
Nifedipine (NIF), as one of the dihydropyridine calcium channel blockers, is widely used in the treatment of hypertension. However, misuse or ingestion of NIF can result in serious health issues such as myocardial infarction, arrhythmia, stroke, and even death. It is essential to design a reliable and sensitive detection method to monitor NIF. In this work, an innovative molecularly imprinted polymer dual-emission fluorescent sensor (CDs@PDA-MIPs) strategy was successfully designed for sensitive detection of NIF. The fluorescent intensity of the probe decreased with increasing NIF concentration, showing a satisfactory linear relationship within the range 1.0 × 10 −6 M ~ 5.0 × 10 −3 M. The LOD of NIF was 9.38 × 10 −7 M (S/N = 3) in fluorescence detection. The application of the CDs@PDA-MIPs in actual samples such as urine and Qiangli Dingxuan tablets has been verified, with recovery ranging from 97.8 to 102.8% for NIF. Therefore, the fluorescent probe demonstrates great potential as a sensing system for detecting NIF. Graphical abstract
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Food Analytical Methods
·
2025
·
IF 3
Zeng Jingxuan,Zheng Haoyue,He Rifeng,Zhou Dan,Peng Xiaoyan,Mo Nan,Mo Zhonghai,Zhou Xue,Ge Fahuan
In this paper, we proposed a new supercritical CO 2 defatting—pH multistage regulation extraction method (SD-PMRE) of high water soluble walnut protein isolate (HWPI), by investigating the effects of PMRE, three defatting methods, and walnut seed coat on the functional properties of HWPI. The results showed that HWPI had high yield of 48.56% and high protein content of 87.58%. More importantly, it had a solubility of 91.58%, significantly higher than walnut protein isolate (WPI) with the highest solubility. Meanwhile, other functional properties of HWPI were greatly improved, with emulsification capacity and stability of 118.07 m 2 /g and 91.03%, and foaming capacity and stability of 129.17% and 89.29%, respectively. Through the SD-PMRE, the problem of WPI’s poor functional properties such as solubility was solved, avoiding the shortcomings brought by modification methods and expanding the application of walnut protein in the fields of food and medicine.
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MICROCHIMICA ACTA
·
2024
·Vol. 192
·期 1
·1-12
·
IF 5.3
Hou Linqian,Wei Jinya,Xiang Chen,Yang Dezhi,Yang Yaling
A novel copper and iron doped containing chitosan and heparin sodium carbon dots (CS-Cu,Fe/HS) nanozyme was formulated through a single-step microwave digestion method. CS-Cu,Fe/HS exhibits excellent peroxidase (POD)-like activity and positive charge characteristics, and it can oxidize the negatively charged 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) in the presence of H 2 O 2 to produce a green compound (ox-ABTS). Furthermore, CS-Cu,Fe/HS enhances electron transfer and provides additional active sites through the valence state transformations of Fe 2+ /Fe 3+ and Cu 2+ /Cu + . Interestingly, the POD-like activity of CS-Cu,Fe/HS is inhibited with the introduction of ampicillin (AMP), which may be because the Cu and Fe ions in CS-Cu,Fe/HS form complexes with AMP, leading to changes in the structure or surface properties of the nanozyme, thereby reducing the number of active sites on the nanozyme. Drawing from this, a straightforward and reliable colorimetric sensor was constructed for AMP detection, featuring a linear range of 0.033 to 110 μg/mL and a detection limit as low as 11.6 ng/mL. The proposed detection method for AMP performed well in real samples, with recoveries ranging from 94.8% to 110.2%. Graphical abstract
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COLLOID AND POLYMER SCIENCE
·
2025
·
IF 2.3
Hu Jun,Shi Lei-ting,Luo Yue,Chen Mao,Jin Cheng,Guo Yong-jun,Yuan Na
Chemical flooding has gained significant attention as an economical and effective technique for enhancing heavy oil recovery. Nevertheless, its application in heavy oil production is often hampered by low sweep efficiency and high injection pressure. To address these challenges, a new oil-displacing agent involving a macromolecular surfactant and a surfactant-polymer compound system has been proposed. This study introduces a surfactant-polymer (PAS) synthesized from acrylamide, 2-acrylamido-2-methylpropane sulfonic acid, and N -hexadecyl- N -sulfo acrylamide, along with a macromolecular surfactant (PSS) made from acrylamide, acrylic acid, sodium p-styrene sulfonate, N -hexadecyl- N -sulfo acrylamide, butyl acrylate, methylene-bis-acrylamide, and nano-silica. The structural composition of PAS and PSS was confirmed through 1 H-NMR and FT-IR analyses. Extensive testing was conducted comparing the performance of PAS, PSS, and a PAS-PSS compound system against an association polymer (AP-1) and a conventional polymer (HPAM). The study focused on thickening performance, interfacial activity, viscosity reduction of heavy oil, and core flooding performance. Results revealed that the PAS-PSS combination significantly outperforms both HPAM and AP-1 in terms of reducing heavy oil viscosity and improving interfacial activity. Specifically, a 1500 mg/L PAS-PSS system achieved a 90.83% reduction in heavy oil viscosity, reduced water–oil interfacial tension to 4.64 mN/m, and had an apparent solution viscosity of 6.45 mPa·s. Oil displacement experiments further demonstrated that the PAS-PSS system enhanced heavy oil recovery by 43.33%, markedly higher than the recovery rates for AP-1 (26.63%) and HPAM (16.76%). Additionally, the compounded system exhibited a lower apparent viscosity, leading to reduced injection pressure. Investigations into the mechanism showed that the PAS-PSS system effectively emulsifies heavy oil to lower its viscosity and increases the viscosity of the water phase. This dual action significantly improves both the oil displacement efficiency and sweep efficiency, subsequently enhancing overall heavy oil recovery. In summary, the use of a surfactant-polymer and a macromolecular surfactant complex system offers a promising advancement in chemical flooding techniques for high-viscosity heavy oil extraction, presenting a novel method to bolster heavy oil recovery operations. Graphical
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Journal of Food Measurement and Characterization
·
2017
·Vol. 12
·期 2
·728-735
·
IF 0.536
Lu Ming,Han Yan-Ping,Zhao Xin-Huai
Gelatin is an important protein-based hydrocolloid, providing excellent gelling properties in processed foods. Horseradish peroxidase (100 U/g protein), glucose oxidase (1 U/g protein), and glucose (0.025 mmol/g protein) were applied to cross-link bovine gelatin at 37 °C for 3 h, and the gelling properties of cross-linked gelatin were evaluated. Obviously, the cross-linking process did not change the composition of amino acids. The cross-linked gelatin had 39.2% increase in surface hydrophobicity, 54.7% increase in emulsion stability index, but 28.2% decrease in emulsifying activity index, in comparison with bovine gelatin. Laser scanning confocal microscopy observation indicated that cross-linked gelatin rather than bovine gelatin conferred better droplet stability on the generated emulsion after long-storage period of 48 h or 7 days. Moreover, cross-linked gelatin had decreased in vitro digestibility during pepsin hydrolysis (38.5%) and pepsin–trypsin hydrolysis (14.5%) than bovine gelatin. Finally, the gelling and melting temperatures of cross-linked gelatin were 2 °C higher than those of bovine gelatin. Overall, the cross-linking process of bovine gelatin led to easier gelation and improved heat resistance of the subsequently gels.
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Scientific Reports
·
2017
·Vol. 7
·期 1
·1-10
·
IF 4.259
Yu Bing,Song Qianqian,Cong Hailin,Xu Xiaodan,Han Dongwei,Geng Zhongmin,Zhang Xiaoyan,Usman Muhammad
In this paper, a thermo- and pH-responsive microfiltration membrane was prepared based on three-dimensional (3D) inverse colloidal crystals (ICC). To manufacture the smart ICC membrane, the typical thermo-responsive N-isopropylacrylamide (NIPAM) and pH-responsive methacrylic acid (MAA) were polymerized inside silica colloidal crystals. The smart ICC membranes were characterized by SEM, IR and contact angle measurements. Moreover, the permeability of smart microfiltration membrane was carried out by the KCl diffusion tests. The result showed that effective diameter of the polymer ICC membrane can be reversible tuned by temperature and pH. Besides, the functional ICC membrane showed outstanding temperature- and pH-responsive gating property, which was applied to separate particles of different sizes. The savvy environment-responsive gating membranes have potential uses in filtration, separation, purification, sensor and other applications.
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Journal of Central South University
·
2017
·Vol. 24
·期 8
·1727-1735
·
IF 0.601
Gao Zheng-cai,Li Li-qing,Li Hai-long,Chen Ruo-fei,Wang S.,Wang Yang-gang
A zeolitic imidazolate hybrid membrane (Co-IM-mIM) containing two imidazolate ligands deposited on a macroporous α-alumina support was prepared by pre-depositing and secondary growth technique. XRD, TGA and SEM characterizations demonstrate that a stable and thin, but dense and pure-phase Co-IM-mIM membrane can be obtained on the macroporous-alumina discs in Teflon-lined autoclave at 120 °C after pre-depositing by dip-coating at room temperature. No visible cracks, pinholes or other defects were observed on the membrane layer. The gas separation studies of Co-IM-mIM membrane were carried out at 25 °C and 1×10 5 Pa, showing ideal selectivity of 6.95, 5.25, 3.40 for H 2 /CO 2 , H 2 /N 2 and H 2 /CH 4 , respectively, and a permeance of 17.37× 10 −6 mol/(m 2 ·s·Pa) for H 2 . The influence of temperature and trans-membrane pressure on hydrogen separation and permeation was also carried out. The gas permeation and selectivity demonstrate that this membrane may have potential applications for efficient H 2 separation.
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MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 8
·2919-2924
·
IF 4.58
He Linghao,Guo Chuanpan,Song Yingpan,Zhang Shuai,Wang Minghua,Peng Donglai,Fang Shaoming,Zhang Zhihong,Liu Chun-Sen
The authors describe an electrochemical immunoassay for the β-adrenergic agonist ractopamine (RAC). It is based on the use of chitosan-stabilized gold nanoparticles (chit-AuNPs). Antibodies against RAC (anti-RAC) readily adsorb on the surface of the chit-AuNPs due to their high bio-affinity. The amount of chitosan used to synthesize chit-AuNPs does not affect the size and distribution of the NPs but amplifies the electrochemical signal of the electrode. The assay has a detection limit as low as 2.3 pg∙mL −1 (equivalent to a 6.7 fM concentration), with a response that is linear in the 0.01 to 5 ng∙mL −1 RAC concentration range. The assay is selective, acceptably reproducible, stable, and well applicable to the detection of RAC.
Chitosan-stabilized gold nanoparticles (chit-AuNPs) were prepared for electrochemical immunoassay of the ractopamine (RAC). The assay exhibits a detection limit of 2.3 pg·mL −1 (equal to 6.7 fM), is highly selective, acceptably reproducible, and stable.
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MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 8
·2909-2917
·
IF 4.58
Han Caiqin,Li Yuqi,Jia Qin,Bradley Layne Hyer,Gan Yun,Yao Yue,Qu Lulu,Li Haitao,Zhao Yiping
An on-demand, disposable and portable surface-enhanced Raman scattering (SERS) array has been prepared by modifying the surface of cellulose paper and patterning it with silver nanoparticles (AgNPs) via pen-writing technique. The paper is initially modified with hexadecenyl succinic anhydride to generate a hydrophobic top layer that is capable of concentrating melamine (and other hydrophobic analytes) by preventing the mainly aqueous sample from spreading on the paper. The AgNPs are subsequently written onto the hydrophobic paper with a high degree of control to form large-scale SERS sensing arrays. The resulting arrays demonstrate outstanding Raman scattering enhancement as demonstrated for the fluorescent dye Rhodamine 6G which can be detected by SERS with an 80 pM limit of detection (LOD) without interference by fluorescence. The reproducibility in the preparation of spots has a relative standard deviation of <15% (for n = 128 spectra). The arrays were applied to the determination of the illicit milk additive melamine. The plot of SERS intensity at Δν = 681 cm −1 versus the logarithm of melamine concentration is linear in the concentration range from 0.3 to 20 mg·L −1 , and the LOD is 0.27 mg·L −1 . In our perception, the disposable arrays presented here integrate the advantages of easy production and reliable reproducibility, and therefore are an ideal choice for use in SERS detection.
An on-demand, disposable and portable surface-enhanced Raman scattering (SERS) array has been developed by pen writing. Such a SERS array can be applied for the highly sensitive determination of the illicit milk additive melamine, which holds great promise for field-deployable applications.
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BIOTECHNOLOGY LETTERS
·
2017
·Vol. 39
·期 8
·1229-1235
·
IF 1.73
Wang Qianqian,Xu Yixiang,Xu Jiaqi,Wang Xudong,Shen Chen,Zhang Yan,Liu Xiufeng,Yu Boyang,Zhang Jian
Objectives To characterize glycosyltransferases from Bacillus subtilis ATCC 6633 and investigate their substrate specificity towards plant polyphenols. Results Among the cloned and expressed six UDP-glycosyltransferases (BsGT1-6), BsGT-1 showed activity with a wide range of polyphenols: morin, quercetin, alizarin, rehin, curcumin and aloe emodin. The gene of BsGT-1 has an ORF of 1206 bp encoding 402 amino acids. The recombinant enzyme was purified to homogeneity by Ni–NTA affinity chromatograph, and its biochemical characteristics were identified by HPLC–UV/MS, 1 H-NMR and 13 C-NMR. BsGT-1 has an MW of approx. 46 kDa as indicated by SDS-PAGE; its activity was optimal at 40 °C and pH 8.5. The Km value of BsGT-1 towards morin was 110 μM. Conclusions BsGT-1 from B. subtilis was cloned. It had high catalytic capabilities towards polyphenols which would make it feasible for the structural modification of polyphenols.
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Food Analytical Methods
·
2017
·Vol. 10
·期 11
·3565-3571
·
IF 2.038
Yue Mei-E,Lin Qiaoyan,Li Qian,Xu Jie,Jiang Ting-Fu
An integrative coupling approach of headspace in-tube microextraction (HS-ITME) and reverse-flow micellar electrokinetic capillary chromatography (RF-MECC) was proposed for determination of phthalic acid esters (PAEs). In the novel method, by placing a capillary filled with background electrolyte (BGE) of RF-MECC in the HS above the sample solution, the PAEs were extracted into the acceptor phase in the capillary. Then, the extracts were separated and determined by RF-MECC. The influence of some essential BGE components such as sodium dodecyl sulfate (SDS) and organic modifier concentrations were investigated. Extraction parameters were also systematically investigated, including the extraction temperature, extraction time, salt concentration, and volume of the sample solution. Under the optimized conditions, enrichment factors for three PAEs were 169, 2534, and 1526, respectively. The proposed method provided a good linearity, low limits of detection, and good repeatability (RSDs below 5.44%, n = 5). This method was then utilized to analyze the three PAEs in tap water, seawater, and beverage samples. The results indicated that the developed method is an excellent alternative for the PAE routine analysis in the food field.
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JOURNAL OF FLUORESCENCE
·
2017
·Vol. 27
·期 4
·1495-1503
·
IF 1.461
Lin Bixia,Yu Ying,Liu Fangfei,Cao Yujuan,Guo Manli
Indole propionic acid (IPA) is one of the important plant growth hormones for promoting rooting and fruiting. Labeling IPA receptor in plant tissues is able to further track the signal transduction processes of IPA and uncover the function mechanism of IPA on crop productions. In this paper, a tunable and nontoxic fluorescent probe for IPA receptors was designed and synthetized base on carbon dots (C dots). Firstly carboxyl-modified carbon dots were prepared by high temperature cracking of citric acid. The fluorescence emission wavelengths of C dots varied with the excitation wavelengths change. Then IPA-modified carbon dots (IPA-C dots) were prepared by coupling the amino of tryptophan with the carboxyl of as-prepared carbon dots. Compared with C dots, the fluorescence intensity of IPA-C dots was double and the fluorescence stability was satisfactory under various conditions. This probe retained the biological activity of IPA and acted as target recognition of IPA receptors in plant tissues. The probe could avoid green fluorescence background of plants. The imaging results showed that the IPA receptors mainly existed on the membrane of stele. The toxicity test indicated the probe was less toxic than traditional inorganic semiconductor quantum dots.
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