Environmental Technology & Innovation
·
2026
·
IF 7.3
Wenjie Wang,Xulong Fan,Yaquan Cao,Ruolin Shi,Ping Wang,Gaofeng Liang
Benzophenone-3 (BP-3), a common ultraviolet (UV) filter widely used in sunscreens, has raised increasing concern because early-life exposure may interfere with normal neurodevelopment. In this study, we fabricated a 3D inverse-opal polyethylene glycol diacrylate (PEGDA) scaffold and established a scaffold-supported brain organoid model for in vitro evaluation of BP-3-induced neurotoxicity. The scaffold was characterized by scanning electron microscopy (SEM), which revealed a highly ordered porous morphology with uniform pore size. It also showed good biocompatibility for organoid culture. BP-3 exposure induced concentration- and time-dependent toxicity in brain organoids, with more pronounced effects observed at 50 and 100 μM after 72 h of exposure. Under these defined exposure conditions, BP-3 was associated with increased oxidative stress, calcium-related functional changes, impaired membrane integrity, increased cytotoxicity, reduced expression of synaptic structural markers, and altered Wnt/β-catenin-related protein expression. These findings suggest that BP-3 exposure may disrupt early neurodevelopment-related processes in this model and is associated with synaptic-related alterations and Wnt/β-catenin dysregulation. Overall, this study supports the applicability of scaffold-supported brain organoids as an in vitro platform for evaluating BP-3-induced neurotoxic responses under controlled experimental conditions.
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高影响因子
ULTRASONICS SONOCHEMISTRY
·
2026
·
IF 10.2
Xinran Niu,Di An,Liang Li
This study systematically investigated the effects of ultrasonic pretreatment on the fibrillation behavior of soy protein isolate (SPI), β-conglycinin (7S) and glycinin (11S). A multi-analytical approach combining Thioflavin T fluorescence spectroscopy, SDS-PAGE, dynamic light scattering, zeta potential measurement, and spectroscopic analyses was employed to monitor fibrillation kinetics and structural modifications. Ultrasonication significantly accelerated the fibrillation process by enhancing nucleation, increasing the apparent rate constant of 7S by 44% and that of SPI by 67%. SDS-PAGE analysis indicated that ultrasound treatment promoted protein degradation, generating a greater number of low-molecular-weight peptides, which may have facilitated fibril nucleation. Ultrasound treatment reduced protein particle size, increased surface charge, and promoted conformational transitions toward β-sheet-rich structures, with U-7SN showing a 20.8% rise in β-sheet content. These structural changes were correlated with enhanced functional properties, including improved solubility, emulsifying activity, and viscosity. The findings demonstrated that ultrasound promoted fibrillation by enhancing hydrophobic interactions and the formation of β-sheet structures, offering a theoretical basis for tailoring plant protein nanofibrils with targeted functionalities for food and material applications.
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CHEMICO-BIOLOGICAL INTERACTIONS
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2026
·
IF 5.2
Jiaqi Li,Zhengxin Lu,Nan Zhang,Xiaowei Zhou,Yuanbiao Ma,Jia-qi Li,Yimu Zhang,Zhewen Deng,Na Lin,Peng Yue,Yilin Yang,Qi Zhang,Bo Ma
Andrographolide (Andro), the primary bioactive compound of Andrographis paniculata , has known bioactivities but also male reproductive toxicity with unclear mechanisms. This study investigated its testicular injury effects and mechanism, focusing on Sertoli cells (SCs) ferroptosis. Toxicity was evaluated in mice and SCs. Mechanisms were probed using RT-qPCR, Western blot, co-immunoprecipitation, and ubiquitination assays. LC-MS/MS assessed tissue distribution. Molecular docking and dynamics simulations characterized the Andro-β-TrCP interaction. The results demonstrated that Andro exposure caused significant testicular atrophy, blood-testis barrier (BTB) disruption, and spermatogenic impairment in mice. Meanwhile, pharmacokinetic analysis revealed SCs as the primary target of Andro-induced testicular damage, showing rapid Andro accumulation that was subsequently detected in sperm and the epididymis. Furthermore, Andro triggered ferroptosis in testicular injury and SCs, marked by increased ROS, MDA, iron overload, and lipid peroxidation. These effects were rescued by the ferroptosis inhibitor deferoxamine. Mechanistically, Andro directly bound to β-TrCP, downregulating its expression and subsequently stabilizing ATF4 by impairing its ubiquitin-mediated degradation. Silencing ATF4 inhibited ferroptosis and restored cell viability upon Andro challenge. The direct Andro-β-TrCP interaction was consistently confirmed by molecular docking, dynamics simulations, and CETSA. This study reveals that Andro triggers SCs ferroptosis by inhibiting β-TrCP-mediated ATF4 ubiquitination and degradation, leading to BTB disruption and spermatogenic impairment. These findings provide a theoretical basis for optimizing clinical application of Andro in the future.
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Longbin Li,Chi Feng,Fuhou Lei,Jiufang Duan,Kun Wang,Jianxin Jiang
β-Cyclodextrin/tea saponin (CT) complexes were prepared. • CT-stabilized eugenol emulsion shows superior storage and environmental stability. • CT complex retards the release rate of eugenol in the composite film. • Cationic guar gum maintained emulsion structural integrity during film formation. • The film integrates antibacterial, UV-shielding, and sustained-release properties. Addressing the severe postharvest losses of perishable fruits and environmental concerns caused by plastic packaging, this study developed a multifunctional composite film. Specifically, a composite of β-cyclodextrin and tea saponin—a byproduct of camellia oil processing—was synthesized and served as a stabilizer for the eugenol emulsion. This composite exhibited favorable surface activity, endowing the emulsion with excellent storage stability. Further, incorporating the emulsion into a κ-carrageenan/cationic guar gum film-forming matrix, the obtained film exhibited favorable mechanical properties (tensile strength: 22.62 MPa, elongation at break: 33.16%), outstanding antioxidant activity (scavenging rates of 60.77% for DPPH and 94.03% for ABTS radicals), and excellent sustained-release effect. Strawberry preservation experiment further confirmed that the composite film can inhibit microbial growth and reduce water loss, thereby extending the shelf life of fruits. This study provides valuable insights for advancing green packaging technologies and promoting the sustainable utilization of food processing byproducts. Download: Download high-res image (142KB) Download: Download full-size image
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Ziteng Huang,Yaru Li,Yuyang Hu,Qiankun Xu,Danya Li,Juzhou Zhang,Xianbiao Wang
Friedel–Crafts acylation yields β-diketone polymer superior to Claisen condensation. • The probe exhibits a vivid green-to-blue shift and intensity enhancement with Al 3+ . • Conformation restriction by Al 3+ coordination enables fluorescence change. • Dual-mode sensing enables both visual screening and precise quantification. • Results in wheat food samples show high consistency with standard ICP-MS methods. Aluminum additives in flour-based foods pose safety concerns and require rapid monitoring. This study presents a high-performance β-diketone main-chain polymer fluorescence sensor synthesized via Friedel–Crafts acylation for specific aluminum detection. This preparation method yielded a polymer with superior sponge-like porosity and extended π-conjugation. Upon Al 3+ coordination, the probe exhibits a distinct fluorescence transition from dim green to brilliant deep blue, enabling visual identification. The response follows a positive cooperative logistic model, achieving a limit of detection of 1.78 μmol/L. Mechanistic studies attribute signal enhancement to a synergistic effect, where coordination restricts the conformational relaxation of the polymer backbone to suppress non-radiative decay while simultaneously increasing radiative oscillator strength. Validated in wheat derivatives with a calibration strategy, the method achieved quantitative results consistent with inductively coupled plasma mass spectrometry. This work highlights the value of backbone-integrated recognition sites and conformational restriction for sensing. A β-diketone main-chain polymer fluorescence sensor addresses the challenge of monitoring aluminum in wheat-based foods. The probe exhibits a positive cooperative logistic response to Al 3+ , characterized by a vivid green-to-blue emission shift. By leveraging a coordination-induced restriction of main-chain conformational relaxation mechanism, the sensor achieves excellent selectivity and sensitivity, allowing for both naked-eye inspection and precise instrumental quantification in real samples. Download: Download high-res image (140KB) Download: Download full-size image
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Shuo Wang,Yanqing Zhang,Mengyu Zhang,Min Wang,Tan Yang,Junbo Xie
Spinosin is an excellent antiglycation agent. • The cross-linked structure and β-amyloid proteins were formed during glycosylation. • The glycation sites protected by Spinosin were determined by molecular docking analysis. • Spinosin quenched the fluorescence of β-lactoglobulin via a static quenching mechanism. Inhibiting advanced glycation end products (AGEs) in thermally processed foods is crucial for managing diabetic complications. This study evaluated the antiglycation activity and mechanisms of Spinosin (SP) from sour jujube seeds in three glycation systems, namely, β-lactoglobulin (β-Lg) with glucose, glyoxal, or glycolaldehyde, incubated at 37 °C in the dark. The results showed that 40–160 μM SP significantly inhibited AGEs formation, with inhibition rates at 160 μM reaching 98.83 ± 0.1%, 83.46 ± 2.1%, and 37.26 ± 0.1% for the glucose, glyoxal, and glycolaldehyde systems, respectively. In addition, SP also inhibited protein oxidation, cross-linking, and the formation of amyloid cross-β structures, potentially contributing to the preservation of β-Lg structure. Molecular docking suggested that SP may shield glycation-prone residues via steric hindrance. Fluorescence titration indicated hydrogen-bonding and van der Waals interactions with a temperature-decreased binding constant (exothermic). These findings suggest that SP is a multi-pathway natural antiglycation agent, but its efficacy in real food matrices requires further validation. Download: Download high-res image (190KB) Download: Download full-size image
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EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2026
·Vol. 252
·期 9
·331
·
IF 4.2
Li Donghui,Gong Jinhua,Liu Shilin,Ran Wenhua,Zhao Jing,Li Yan
Water-in-oil-in-water (W1/O/W2) emulsions are capable of co-encapsulating hydrophilic and hydrophobic compounds, but their practical application is often limited by poor physicochemical stability. In this study, a W1/O/W2 emulsion was developed by stabilizing the internal and external aqueous phases with chitosan (CS) and whey protein concentrate (WPC), respectively, while incorporating cinnamaldehyde (CA) into the intermediate oil phase for potential dual-interface stabilization through interfacial interactions. Optimization of the phase composition led to an emulsion with significantly improved storage stability (extending its shelf-life from 7 to 60 days) and enhanced tolerance ability against pH and ionic strength variations. The optimized emulsion successfully co-encapsulated tea polyphenols (TPPs) and β-carotene (BC), maintaining high bioactive compound levels and preserving antioxidant activity under varying pH and thermal conditions. In vitro gastrointestinal digestion assays revealed that CA-modified emulsions significantly improved the bioaccessibility of TPPs and BC to 61.72% and 48.72%, respectively, corresponding to approximately 3.1-fold and 2.0-fold increase compared to their free form. Moreover, the co-encapsulation system exhibited higher antioxidant capacity. This study provides a promising strategy for improving the stability and co-delivery performance of hydrophilic and lipophilic bioactive compounds, with potential applications in development of functional and value-added foods.
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JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2026
·
IF 4.4
Mengchao Feng,Yuchang Wang,Xuewei Lang,Feng Wei,Huayan Si,Jianbin Zhang
This work reports the synthesis of a novel ( α / β- CdS)/Ce(OH)CO 3 composite featuring both heterojunction and heterogeneous junction structures via a simple injection titration-stirring method. The as-prepared composite exhibited excellent visible-light-driven photocatalytic activity for the degradation of high-concentration oxytetracycline (OTC) solution. Under optimal conditions determined by single-factor and response surface analyses, 0.0300 g of the composite achieved a 75.26% degradation efficiency for 50 mL of 160 mg/L OTC solution within 120 min of visible light irradiation. Notably, the composite demonstrated remarkable stability, retaining 62.39% of its initial activity after five cycles, and showed enhanced performance under real sunlight for 120 min, with a degradation rate of 78.50%. The improved photocatalytic performance is attributed to the uniform dispersion of CdS nanoparticles with abundant sulfur vacancies, which increase light absorption and facilitate charge carrier separation. Furthermore, the formation of direct Z -scheme heterojunctions and heterogeneous junctions between CdS and Ce(OH)CO 3 promotes efficient separation and transport of photogenerated carriers, thereby enhancing both photocatalytic activity and material stability.
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高影响因子
XENOBIOTICA
·
2026
·
IF 1.5
Jiexin Qiu,Beibei Yao,Qing Yu,Jiajie Wang,Lingling Huang,Jiale Chen,Yufeng Lu,Chunying Wang
Cis-2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside (cis-THSG), a photoisomer of the major bioactive component in Polygoni Multiflori Radix Praeparata (He-Shou-Wu), has enhanced hypolipidemic activity but is associated with idiosyncratic hepatotoxicity. Its metabolic fate under pathological conditions and key clearance enzymes remain unclear.This study aimed to compare metabolite profiles of cis-THSG in normal and hyperlipidemic rats in vivo and in vitro, identify metabolites, and characterise enzyme kinetics in rat liver microsomes (RLMs) and major CYP450 isoforms. Metabolites in plasma, urine, faeces, bile, and intestinal bacteria incubation fluids were analysed by UHPLC-Q-TOF-MS/MS. Enzymatic kinetics and CYP450 isoforms were investigated via chemical inhibition assays.A total of 27 metabolites were characterised; cis-THSG underwent rapid glucoside hydrolysis, and its aglycone underwent phase I and II reactions. Hyperlipidaemia reduced metabolites to 20 and markedly increased plasma cis-THSG and aglycone abundance. Kinetic parameters were Km 32.86 μmol/L, Vmax 0.7285 μmol/(min·mg protein), CLint 0.02217 L/(min·mg protein). Rat liver microsomal CYP isoforms analogous to human CYP3A4 and CYP2C9 mediated cis-THSG oxidative metabolism.This study provides the first comprehensive metabolite map of cis-THSG, identifies its aglycone as a potential bioactive form, and reveals a disease-related metabolic shift. CYP phenotyping offers a mechanistic basis for predicting clinical drug–drug interactions.
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高影响因子
ChemistrySelect
·
2026
·Vol. 11
·期 36
·e74500
·
IF 2.3
Cong Zhu,Tianqi Li,Wenhao Liu,Teng Wang,Mengjing An,Yunjun Luo
Multi-hydrogen bond chain extenders are widely used to improve the mechanical properties of polyurethane elastomers, but their incorporation often requires polar organic solvents, limiting solvent-free processing and large-scale production. Herein, a one-step solvent-free strategy was developed by dispersing β-hydroxyethylurea (2-Hu) in low-molecular-weight polyethylene glycol (PEG) to construct a mixed chain-extending system for hydroxyl-terminated polycaprolactone polyether (HTCE)-based polyurethane. Fourier transform infrared spectroscopy (FTIR) confirmed successful polyurethane formation, while small-angle x-ray scattering (SAXS) and low-field nuclear magnetic resonance (LFNMR) revealed that 2-Hu enhanced hydrogen bonding, promoted hard-domain aggregation, and strengthened microphase separation. Density functional theory (DFT) calculations demonstrated stronger intermolecular hydrogen-bonding interactions for 2-Hu than for 1,4-butanediol (BDO). Consequently, the crosslink density increased from 3.06 × 10 − 4 to 3.41 × 10 − 4 mol·mL − 1 , and the tensile strength improved from 0.86 to 1.00 MPa. Moreover, the 2-Hu-containing polyurethane exhibited complete elastic recovery after 10 loading–unloading cycles, demonstrating excellent reversible deformation enabled by the dynamic hydrogen bonding network. This solvent-free strategy provides a simple and scalable route for preparing polyurethane elastomers containing multi-hydrogen bond interactions. Graphical 2-Hu is dispersed in polyethylene glycol (PEG) and then incorporated into polyurethane systems. The formation of multiple hydrogen bonds creates a dynamic crosslinked network. This structural modification effectively enhances the overall mechanical performance, including improved strength and toughness. The strategy offers a facile route to develop high-strength polyurethane elastomers with tunable properties.
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高影响因子
PLoS One
·
2025
·Vol. 20
·期 6
·e0326978
·
IF 2.6
Chang Chen,Qiqi Yun,Juanli Ran,Ziyao Zhou,Pengxiang Zhang,Rong Li
Objective
This study investigated the interplay between Hippo-YAP and β-catenin signaling in regulating odontogenic differentiation of human dental pulp stem cells (DPSCs) and explored its potential implications for dentin-pulp regeneration.
Methods
Using lentivirus-mediated YAP overexpression/silencing, β-catenin siRNA knockdown, and pharmacological Wnt inhibition (via WIF-1), we assessed DPSC proliferation, migration, mineralization, and molecular markers (via qRT-PCR, immunofluorescence). In vivo validation employed subcutaneous transplantation of DPSC-seeded scaffolds in immunocompromised mice.
Results
YAP activation enhanced DPSC proliferation (1.44-fold), migration (1.39-fold), invasion (1.54-fold), and differentiation, as evidenced by elevated ALP activity (1.46-fold) and mineralization (1.36-fold). We observed transcriptional upregulation of odontogenic markers (RUNX2, DSPP, DMP1, OCN, ALP) and Wnt pathway components (β-catenin, Cyclin D1, c-Myc). Immunofluorescence revealed coordinated YAP and β-catenin expression patterns during differentiation. β-catenin silencing or Wnt inhibition abolished YAP-mediated functional enhancements and simultaneously suppressed YAP expression, partially confirming bidirectional regulation. In vivo , YAP-overexpressing DPSCs exhibited 1.27- to 1.62-fold induction of dentin-specific markers and β-catenin, whereas YAP silencing impaired these markers expression.
Conclusions
Our findings demonstrate that coordinated YAP and β-catenin signaling drives DPSC odontogenesis, with potential implications for dentin regeneration. Although reciprocal regulation is evident, direct molecular interactions require further validation.
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BIOCHEMICAL PHARMACOLOGY
·
2025
·
IF 5.6
Xiaoshen Zhang,Tao Jiang,Lingyun Ye,Jianguo Sun,Lei Wang,Juanjuan Li,Fengying Wu,Shengxiang Ren,Guanghui Gao
Non-small cell lung cancer (NSCLC) accounts for 85% of lung cancer cases, posing a major health burden. Our prior work indicated fibronectin promotes NSCLC angiogenesis and progression by upregulating Wnt-inducible signaling pathway protein 3 (WISP3) and activating Wnt signaling. This study aims to explore WISP3 ′ s role and mechanisms in NSCLC progression. Cells were transfected with a reconstructed plasmid to study gene overexpression/silenced effects. Functional assays were conducted, including tube formation, sphere formation, immunohistochemistry, western blotting, Transwell, and MTT. Mass spectrometry and bioinformatics analysis were used to identify differentially expressed proteins and their associated signaling pathways in NSCLC cells. Additionally, the xenograft tumor model of NSCLC was constructed. High expression of WISP3 in NSCLC is associated with poor prognosis, as it enhances angiogenic and tumorigenic potential in NSCLC cells, further verified in our constructed xenograft tumor experiment. In terms of mechanisms, the activation of the TGF-β signaling pathway, indicated by p-smad2/3 activation, is implicated in the progression of NSCLC promoted by WISP3. Additionally, overexpression of WISP3 leads to increased syntenin binding protein (SDCBP) expression in vitro and in vivo , and WISP3 binding to SDCBP enhances angiogenic and tumorigenic potential in NSCLC, which can be counteracted by silencing SDCBP, indicating their crucial role in NSCLC progression. We offer new insights into WISP3 ′ s role in promoting NSCLC progression, showing it upregulates SDCBP and is involved in TGF-β signaling. The findings contribute to a better understanding of NSCLC biology and suggest potential therapeutic targets for the disease.
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