Frontiers in Neurology
·
2022
·
IF 4.086
Ren Siying,Han Shanshan,Wang Likun,Huang Yuanxin,Wu Jing,Wu Guofeng
Objective: To investigate the role of minimally invasive surgery (MIS) in intracerebral hemorrhage (ICH) evacuation combined with deferoxamine (DFX) treatment on perihematomal tight junction protein (claudin-5 and ZO-1) expression levels and blood-brain barrier (BBB) permeability in rabbits.Methods: We randomly assigned 65 male rabbits (weight: 1.9–2.6 kg) to a normal control group (NC group, 13 rabbits), hemorrhage model group (HM group, 13), DFX treatment group (DFX group, 13 rabbits), MIS group (MIS group, 13 rabbits), or MIS combined with DFX treatment group (MIS + DFX group, 13 rabbits). ICH was established in all of the groups except the NC group. MIS was performed to evacuate the hematoma 6 h after the ICH model was created in the MIS and MIS + DFX groups. The DFX and MIS + DFX groups were treated with DFX (100 mg/kg, dissolved in 2 mL of 0.9% saline solution, administered intramuscularly) at 2 h, and then every 12 h for 7 d. The same dose of 0.9% saline solution was administered to the NC, HM, and MIS groups at the same time points. Sixty-five rabbits were divided into 5 groups, and 13 rabbits in each group. Neurological deficit (i.e., Purdy's score) was recorded in all rabbits before euthanasia (N total = 65). In each group, 2 rabbits were used for iron concentration measurement (N total = 10), 2 rabbits were used for brain water content measurement (N total = 10), 3 rabbits were used for BBB permeability measurement (N total = 15), 3 rabbits were used for claudin-5, ZO-1 expression detection by Western Blotting (N total = 15), and 3 rabbits were used for claudin-5, ZO-1 mRNA detection by real-time PCR (N total = 15). On day 7, the rabbits were sacrificed and the perihematomal brain tissue was harvested to test the iron concentration, brain water content (BWC), tight junction proteins (claudin-5 and ZO-1) expression, and BBB permeability.Results: Purdy's score, iron concentration, and BWC were lower in the MIS and MIS + DFX groups compared to the HM and DFX groups. The MIS + DFX group showed a significant decrease in these indicators. The use of MIS to evacuate the hematoma led to increased expression levels of claudin-5 and ZO-1, as well as decreased BBB permeability. The MIS + DFX group exhibited a remarkable increase in claudin-5 and ZO-1 expression levels and a significant decrease in BBB permeability.Conclusions: MIS combined with DFX treatment could increase the expression levels of perihematomal tight junction proteins (claudin-5 and ZO-1) expression, reduce BBB permeability, and improve the neurological function. MIS combined with DFX treatment may also prevent secondary brain damage following ICH.
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FOOD AND CHEMICAL TOXICOLOGY
·
2023
·
IF 4.3
Huiying Huang,Jian Shi,Zhongyi Li,Yifeng Rang,Weiye Li,Xueman Xiao,Congying Chen,Chunhong Liu
Nonylphenol (NP) exposure can trigger neurotoxicity and cause learning and memory impairment. Nicotinamide mononucleotide (NMN) has a therapeutic effect on neurodegenerative diseases , but the role of NMN on NP-induced learning and memory impairment is not known. Here, we examined the mitigative effect of NMN on the impaired learning and memory ability of rats exposed to NP. The NP impaired learning and memory in rats, while the low-dose intervention with NMN significantly prolonged the step-through latency of the PAT and improved the NAMPT and NMNAT1 content in brain tissue. At the same time, the NMN intervention also increased the content of 5-HTR 1A , 5-HTR 4 , and 5-HTR 6 related to learning and memory in the hippocampus. In line with this, we found that the NMN intervention activated the SIRT1/MAO-A pathway in brain tissue. NMN intervention, especially at 125 mg/kg doses, may improve rats’ NP-induced learning and memory impairment via the central 5-HT system and the NAD + /SIRT1/MAO-A pathway in the brain.
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ENERGY
·
2021
·
IF 7.147
Liangdong Hu,Longlong Ma,Guangzhi Hu,Wenjie Zhang,Ying Liu,Rui Xu,Wen Ge,Yubao Chen
Under simultaneous illumination and heating, Jatropha oil was converted into jet–fuel components over modified catalysts. XRD , HRTEM, N 2 adsorption–desorption, XRF , NH 3 /CO–NH 3 –TPD/CO–TPD, UV–Vis and TG–DTG–MS were employed to determine the surface morphology and acidic site content of the modified catalysts. Through the characterizations and experimental results, the band gap of the catalysts modified with titanium dioxide was found to be significantly reduced, which enhanced the visible light adsorption of the catalysts. Moreover, the catalyst loaded with Pt contained the highest amount of weakly acidic sites, which led to the highest conversion and selectivity for the Pt/TiO 2 –HZSM–5 catalyst at 100 °C and 0.4 MPa H 2 pressure. In addition, the pathway of photocatalytic decarboxylation and catalytic cracking of Jatropha oil was found to involve a cascade reaction from fatty acid to C n–1 alkane and eventually to cracking products.
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NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 25
·11213-11221
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IF 3.591
Yan Zhong,Ru-Quan Ren,Lei Qin,Jian-Bo Wang,Yi-Yi Peng,Qiang Li,Yong-Ming Fan
Electrochemical oxidation of 5-hydroxymethylfurfural (HMF) to 2,5-furandicarboxylic acid (FDCA) has received increasing attention as it is an eco-friendly strategy for synthesizing a key monomer for the production of bio-based plastics. Developing efficient, low-cost and stable electrocatalysts is crucial for this electrochemical reaction. In this work, we have reported a facile process for the synthesis of hybrid nanosheet-structured NiCo2O4 on carbon fiber paper (NiCo2O4-CFP) for the efficient electrochemical conversion of HMF into FDCA. As a non-noble binary metal-based catalyst, NiCo2O4-CFP featured a nanosheet network and 3D nanosheet flower-like microspheres, showing 94.3% selectivity and 89.6% faradaic efficiency (FE) for FDCA, which outperformed its single component metal oxides NiO-CFP and Co3O4-CFP. And it also exhibited relatively high stability by maintaining over 80% FE after four successive cycles. The proposed mechanism suggested that the synergistic effect of Ni and Co elements in NiCo2O4 could facilitate HMF oxidation.
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Scientific Reports
·
2020
·Vol. 10
·期 1
·1-14
·
IF 3.998
Zhang Chao,Zhang Fanghua,Han Mengnan,Wang Xuming,Du Jie,Zhang Honglei,Li Wei
Combination chemotherapy is still of great importance as part of the standard clinical care for patients with HER2 positive breast cancer. As an attractive component, gold nanoparticles (AuNPs) have been extensively studied as biosafety nanomaterials, but they are rarely explored as drug nanocarriers for targeted co-delivery of multiple chemotherapeutics. Herein, a novel affibody-DNA hybrid strands modified AuNPs were fabricated for co-loading nucleoside analogue (5-fluorodeoxyuridine, FUdR) and anthracycline (doxorubicin, Dox). FUdRs were integrated into DNA hybrid strands decorated on AuNPs by DNA solid phase synthesis, and Dox molecules were intercalated into their duplex regions. Affibody molecules coupled to the DNA hybrid strands were distributed the surface of AuNPs, giving them targeting for HER2. The new dual-drug-containing affibody-DNA-AuNPs (Dox@affi-F/AuNPs) owned compact and stable spherical nanostructures, and precise drug loading. Cytotoxicity tests demonstrated that these nanoparticles caused a higher inhibition in HER2 overexpressing breast cancer cells, and showed better synergistic antitumor activity than simple mixture of the two drugs. The related mechanistic studies proved that Dox@affi-F/AuNPs achieved a remarkable combined antitumor activity of Dox and FUdR by promoting more cells to enter apoptosis pathway. Our work provided a nanomedicine platform for targeted co-delivery of nucleoside analog therapeutics and anthracycline anticancer drugs to achieve synergistic treatment of HER2 + cancer.
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FUEL
·
2020
·
IF 5.578
Chaoxin Yang,Xiao Li,Zhenzhou Zhang,Bohan Lv,Jiachun Li,Zhenjian Liu,Wanzhen Zhu,Furong Tao,Guangqiang Lv,Yongxing Yang
The oxidation of 5-hydroxylmethylfurfural (HMF) to 2,5-furandicarboxylic acid (FDCA) is a sustainable and promising method for the preparation of bio-derived aromatic polyesters . Nitrogen doped carbon material is an effective catalyst support in preparing supported nanoparticle metal catalysts in heterogeneous reactions . In this work, by pyrolysis of bio-waste carbon material, pomelo peel, under 800 °C flowing NH 3 , high nitrogen (up to 11.4 wt%) doped porous carbon material (NC) was facilely obtained. Pt nanoparticles (NPs) can be uniformly dispersed on NC surface and stabilized by the doped nitrogen dopant . Supported Pt NPs by NC-800 (Pt/NC-800) was proved to be an efficient catalyst in aerobic oxidation of HMF into FDCA. Control experiments show that the oxidation of 5-hydrox-ymethyl-2-furancarboxylic acid (HMFCA) to 5-formyl-2-furancarboxylic acid (FFCA) by supported Pt NPs was the key step to determine the oxidation rate of HMF to FDCA. With controlled experiment results and previous reports, a catalytic mechanism involving water, molecular O 2 , hydroxyl ions and Pt NPs was given and explained well of the phenomenon of high HMFCA concentration accumulation during reaction.
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EUROPEAN JOURNAL OF PHARMACOLOGY
·
2021
·
IF 4.432
Fujing Wang,Rong Hu,Jiaxing Zhang,Tingting Pei,Zhuo'en He,Liliang Ju,Zhongxiao Han,Mingqing Wang,Wei Xiao
Renal fibrosis is the pathological consequence of progressive chronic kidney disease . Although it has been reported that vitamin D3 exerts antifibrotic effects, the underlying mechanisms remain unclear. This study is aimed at investigating the effects and molecular mechanisms in high-dose vitamin D3 treatment on renal fibrosis. A model of chronic kidney disease was established by 5/6 nephrectomy in rats characterised by high levels of serum creatine, urea nitrogen, and urinary protein. Serum 25-dihydroxyvitamin D3, calcium and parathormone levels were measured to evaluate vitamin D levels. Hematoxylin and eosin , periodic acid Schiff and Mallory's Trichrome staining were used to evaluate histopathological changes in rats. Moreover, the expression of vimentin , collagen I, α-smooth muscle actin and E-cadherin were analyzed at molecular and histopathological levels. Our results showed that exposure to vitamin D3 decreased the levels of serum creatine, urea nitrogen and urine protein and restored the homeostasis of calcium and parathormone. Vitamin D3 also downregulated the expression of vimentin, collagen I and α-smooth muscle actin and attenuated renal fibrosis and epithelial to mesenchymal transition in the kidney. Importantly, vitamin D3 treatment increased the expression of the vitamin D receptor and inhibited Transforming growth factor-β1 (TGF-β1)/Smad3 signaling pathway in rats kidneys with chronic kidney disease. Mechanistically, the upregulation of TGF-β1 and phosphorylation of Smad3 induced by vitamin D3 was reversed by activation of the vitamin D receptor. Our findings indicated that vitamin D3 is a potential antifibrotic drug in chronic kidney disease via the vitmin D receptor and inhibiting TGF-β1/Smad3 signaling pathway.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Zhisheng Zhou,Miao Zhu,Guowen Zhang,Xing Hu,Junhui Pan
5−Hydroxymethyl−2−furaldehyde (5-HMF) in dairy food products generated via Maillard reaction may bring health risks. Herein, the interaction of 5-HMF with β-casein (β-CN) and its influences on the structure and function of β-CN were investigated. 5-HMF was found to quench the inherent fluorescence of β-CN by a static mode and the binding constant was in the order of magnitude of 10 4 L mol −1 . Thermodynamic analysis indicated that hydrophobic forces played the major role in the interaction. The results of synchronous and three-dimensional fluorescence revealed that 5-HMF changed the microenvironment of tyrosine and tryptophan residues and interfered the stability of the polypeptide backbone structure in β-CN. Molecular docking revealed that 5-HMF bound to the hydrophobic cavity of β-CN mainly interacting with the amino acid residues Pro196, Val155, Leu163, Phe157 and Leu192. The binding of 5-HMF with β-CN caused partial unfolding of β-CN conformation, resulting in the increase in surface hydrophobicity and emulsifiability, and decrease in emulsification stability of β-CN, thereby reducing the solubility of β-CN. The study might facilitate the comprehensive understanding of 5-HMF affecting the structure and functional properties of β-CN.
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高影响因子
Nanomaterials
·
2019
·Vol. 9
·期 2
·290
·
IF 4.034
Weicheng Xu,Kaichun Lin,Daiqi Ye,Xueding Jiang,Junxing Liu,Yangda Chen
In this study, a series of HZSM-5 catalysts were prepared by the chemical liquid-phase deposition method, and low concentration toluene degradation was carried out in an atmospheric pressure dielectric barrier discharge (DBD) reactor. The catalysts were characterized by X-ray powder diffraction (XRD), SEM, TEM, and N2adsorption analysis techniques. In addition, several organic contaminants were used to evaluate the adsorption performance of the prepared catalysts, and the effect of pore size on the removal efficiency of toluene and byproduct formation was also investigated. The unmodified HZSM-5 zeolite (Z0) exhibited good performance in toluene removal and CO2selectivity due to the diffusion resistance of ozone and the amounts of active species (OH• and O•). Meanwhile, the time of flight mass spectrometry (TOF-MS) result showed that there were more byproducts of the benzene ring in the gas phase under the action of small micropore size catalysts. Moreover, the surface byproducts were detected by gas chromatography–mass spectrometry (GC-MS).Keywords:HZSM-5;dielectric barrier discharge;toluene;pore;byproduct
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高影响因子
CATALYSIS TODAY
·
2019
·
IF 4.888
Tianyu Gao,Yongxuan Yin,Guanghui Zhu,Qiue Cao,Wenhao Fang
Oxidative transformations of HMF are key routes toward a wide variety of chemicals and biofuels through an environmental friendly process. Co 3 O 4 NPs decorated Mn-Co-O solid solution, donated as Co 3 O 4 /Mn X Co, was prepared by co-precipitation method and used as non-precious metal catalyst for base-free oxidation of HMF to FDCA in the presence of O 2 and water. Comprehensive characterizations such as XRD, H 2 -TPR, O 2 -TPD, XPS, NH 3 /CO 2 -TPD and TEM were used to study materials properties especially the oxygen mobility and acidity-basicity. The effect of Mn/Co molar ratio in catalyst and the reaction conditions were investigated and optimized. In addition, the kinetics of the reaction was monitored to reveal the reaction route and rate-determining step. The variation of Mn/Co molar ratio was found to affect not only HMF conversion but also distribution of products. When the Mn/Co ratio was of 0.2, the Co 3 O 4 /Mn 0.2 Co catalyst showed the optimal catalytic performance (i.e., >99% yield of FDCA obtained under 1 bar O 2 at 140 °C after 24 h) due to the highest content of both Lewis and Brønsted acid sites on the surface, leading to the strongest ability of HMF adsorption and COOH group formation, as well as the enhanced oxygen mobility. This catalyst was shown stable after a minor deactivation (≤8%) during six recycling uses and its activity can be entirely regenerated by calcination in air.
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JOURNAL OF POWER SOURCES
·
2019
·
IF 7.467
Xiao Zeng,Zhanhong Yang,Jinlei Meng,Linlin Chen,Hongzhe Chen,Haigang Qin
The porous ZnO/C composites derived from metal organic framework-5 (MOF-5) is obtained via a calcination method subsequent to hydrothermal process. The results of materials characterization analysis show that the porous ZnO/C composites is well-crystallized with cube morphology and possesses a large specific surface area. According to the electrochemical measurements, the porous ZnO/C composites as anodic materials for Nickel–Zinc rechargeable battery exhibits excellent electrochemical performance compared with that of the commercial ZnO, keeping the discharge specific capacity at 587 mAh g −1 with the capacity retention ratio of about 89.1% after 800 cycles. The outstanding electrochemical property of porous ZnO/C composites can be ascribed to the interconnected conductive network generated by the carbon layer and stable cube architectures derived from metal organic framework-5 precursor, which can facilitate the transportation of electrons and restrain the formation of zinc dendrites during repeated charge-discharge cycling. Furthermore, a novel sphere structure consisting of numerous intersecting disk-like sheets is transformed from the cubic porous ZnO/C composites after 600 cycles, which can contribute to the uniform current distribution and improve the performance of energy storage, revealing the truth for the long cycle life of porous ZnO/C composites.
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NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
·
2019
·Vol. 393
·期 6
·967-978
·
IF 2.058
Rong Rong,Zhang Rui-zhen,Wang Xin,Dan Yu-han,Zhao Yun-li,Yu Zhi-guo
R -/ S -2-(2-hydroxypropanamido) benzoic acid ( R -/ S -HPABA), marine-derived anti-inflammatory antiplatelet drugs, were initially synthesised in our group. However, preliminary research showed that R -/ S -HPABA were eliminated rapidly because of extensive hydroxylation metabolism of phenyl ring in vivo. In order to reduce significant hydroxylation metabolism to improve pharmacological activity and bioavailability, trifluoromethyl group was incorporated into R -/ S -HPABA to synthesise R -/ S -2-(2-hydroxypropanamido)-5-trifluoromethyl benzoic acid ( R -/ S -HFBA), respectively. The purposes of this study were to report the synthesis of R -/ S -HFBA and compare the anti-inflammatory antiplatelet effect and pharmacokinetic properties of R -/ S -HFBA with those of R -/ S -HPABA. Carrageenan-induced rat paw edema assay was used for the evaluation of the anti-inflammatory activity. R -/ S -HFBA showed better results in inhibiting edema and were able to prolong the anti-inflammatory effect after carrageenan injection. The antiplatelet aggregation activity of R -/ S -HFBA and R -/ S -HPABA was studied on arachidonic acid-induced platelet aggregation of rabbit platelet-rich plasma. The aggregation inhibition rate of R- / S -HFBA was significantly ( p < 0.05) higher than that of R- / S -HPABA, respectively. Molecular docking study revealed that R -/ S -HFBA possess more potent binding affinity with COX-1/COX-2 than R -/ S -HPABA, respectively, and that the presence of trifluoromethyl group leads to increase in activity of R -/ S -HFBA. R -/ S -HFBA also afford more favorable pharmacokinetic properties than R- / S -HPABA, respectively, such as higher C max , larger AUC 0-∞ , and longer t 1/2 , which, as expected, are more metabolically stable.
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危险品化学品经营许可证（带存储）