ELECTROCHEMISTRY COMMUNICATIONS
·
2025
·
IF 4.2
Fang Liu,Yingying Gao,Ying Guo,Jianchun Li,Wei Huo,Weiwei Dai
Cardiac troponin I (cTnI) detection plays a critical role in the early diagnosis of acute myocardial infarction (AMI). In this study, a sandwich-type electrochemical immunosensor was developed for ultrasensitive quantification of cardiac troponin I (cTnI). The sensor platform utilized silver nanoclusters (AgNCs) incorporated with β -cyclodextrin functionalized 3D porous graphene ( β -CD@3DG-AgNCs) to enhance electrical conductivity and primary antibody (Ab 1 ) immobilization. For signal amplification, ferrocenecarboxylic acid combined with β -CD@3DG ( β -CD@3DG-Fc-COOH) was employed as the signal probe, which demonstrated excellent secondary antibody (Ab 2 ) capture capability. The synthesized nanomaterials were characterized using SEM, TEM, FTIR spectroscopy, X-ray Photoelectron Spectroscopy (XPS) and Thermo-Gravimetric Analysis (TGA). The electrochemical performance of the surface-modified electrodes was systematically investigated through cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under optimized conditions, differential pulse voltammetry (DPV) revealed that the developed sensor exhibited an extensive linear response range (100 fg mL −1 –100 ng mL −1 ) for cTnI quantification, with an exceptionally low detection limit of 45.5 fg mL −1 . The recovery rate ranging from 97.39% to 102.83% was achieved in human serum analysis, suggesting the potential applicability of this immunosensor for clinical diagnosis.
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Journal of the Mechanical Behavior of Biomedical Materials
·
2024
·
IF 3.3
Chao Yao,Prisana Pripatnanont,Junbiao Zhang,Srisurang Suttapreyasri
One strategy to correct alveolar bone defects is use of bioactive bone substitutes to maintain the structure of defect site and facilitate cells and vessels' ingrowth. This study aimed to fabricate and characterize the freeze-dried bone regeneration scaffolds composed of polymeric Type I collagen, nano Beta-tricalcium phosphate (β-TCP), and gelatin. The stable structures of scaffolds were obtained by thermal crosslinking and EDC/NHS ((1-ethyl-3-(3-dimethylaminopropyl) carbodiimide)/(N-hydroxysuccinimide)) chemical crosslinking processes. Subsequently, the physicochemical and biological properties of the scaffolds were characterized and assessed. The results indicated the bioactive composite scaffolds containing 10% and 20% (w/v) nano β-TCP exhibited suitable porosity (84.45 ± 25.43 nm, and 94.51 ± 14.69 nm respectively), a rapid swelling property (reaching the maximum swelling rate at 1 h), excellent degradation resistance (residual mass percentage of scaffolds higher than 80% on day 90 in PBS and Type I collagenase solution respectively), and sustained calcium release capabilities. Moreover, they displayed outstanding biological properties, including superior cell viability, cell adhesion, and cell proliferation. Additionally, the scaffolds containing 10% and 20% (w/v) nano β-TCP could promote the osteogenic differentiation of MC3T3-E1. Therefore, the bioactive composite scaffolds containing 10% and 20% (w/v) nano β-TCP could be further studied for being used to treat alveolar bone defects in vivo.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
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IF 6.2
Lili Qi,Shun Zhang,Feiqiang Cao,Yuxiang Li,Zhijian Ke,Mengting Wang,Haiguang Mao,Wei Ma,Yong Yu,Jinbo Wang
Resveratrol production via enzymatic hydrolysis of polydatin is hindered by the thermal instability of β-glucosidases. This study employed rational design to engineer Bacillus sp. D1-derived BglD2 through surface charge optimization. The D156E mutant achieved a 42.7% increase in catalytic efficiency (kcat/Km = 49.76 s–1·mM–1) toward polydatin and a 2.1-fold longer half-life at 40 °C. The D411E mutant exhibited a 5.2-fold improvement in thermostability while retaining native activity. Structural analyses revealed that D156E formed a stabilizing salt bridge (2.7 Å) with Lys111 and a hydrogen bond with Pro112, while D411E established compensatory hydrogen bonds with Phe408. Both mutants maintained broad pH activity (pH 6.0–7.0), high glucose tolerance (Ki > 55 mM), and metal ion resilience. This work demonstrates that targeted surface charge engineering concurrently enhances thermostability and catalytic efficiency, enabling efficient resveratrol production with reduced enzyme consumption in industrial applications.
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APPLIED SURFACE SCIENCE
·
2025
·
IF 6.9
Jiehao Li,Meng Ning,Yi-lei Li,Rui-hong Liu,Haiyang Gao,Ying Liu,Yingjuan Hao,Hui-ying Mu,Fa-tang Li
The construction of heterojunction nanomaterials offers a viable solution to enhance degradation capabilities. In this work, in-situ 2D/2D β-Bi 2 O 3 /Bi 5 NO 3 O 7 extra-low van der Waals (VDW) heterojunctions with oxygen vacancies are prepared by a simple solution combustion method. The successful preparation of extra-low VDW heterojunctions are verified by X-ray diffraction (XRD), high-resolution transmission electron microscopy (HRTEM), X-ray photoelectron spectroscopy (XPS), Density Functional Theory (DFT), etc. The carrier flow direction is determined by DRS, Mott-Schottky, Kelvin probe and femtosecond transient absorption spectra (fs-TAS) characterization. The rate of photocatalytic degradation of Rhodamine B under visible light by the 5.6 β-Bi 2 O 3 /Bi 5 NO 3 O 7 (5.6 BBNO) heterojunction is 1.59 times that of Bi 5 NO 3 O 7 and 3.28 times that of β-Bi 2 O 3 . This is attributed to the extra-low van der Waals heterojunction, which not only enhances the separation efficiency of photogenerated carriers but also lowers the valence band maximum of Bi 5 NO 3 O 7 . Furthermore, capture agent experiments and EPR tests confirm that holes (h + ) play a key role in photocatalytic degradation.
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Biosensors-Basel
·
2025
·Vol. 15
·期 1
·52
·
IF 5.6
Qi Yuan,Yunqing Wang
High-sensitivity and repeatable detection of hydrophobic molecules through the surface-enhanced Raman scattering (SERS) technique is a tough challenge because of their weak adsorption and non-uniform distribution on SERS substrates. In this research, we present a simple self-assembly protocol for monolayer SERS mediated by 6-deoxy-6-thio-β-cyclodextrin (β-CD-SH). This protocol allows for the rapid assembly of a compact silver nanoparticle (Ag NP) monolayer at the oil/water interface within 40 s, while entrapping analyte molecules within hotspots. The proposed method shows general applicability for detecting hydrophobic molecules, exemplified as Nile blue, Nile red, fluconazole, carbendazim, benz[a]anthracene, and bisphenol A. The detection limits range from 10−6to 10−9M, and the relative standard deviations (RSDs) of signal intensity are less than 10%. Moreover, this method was used to investigate the release behaviors of a hydrophobic pollutant (Nile blue) adsorbed on the nanoplastic surface in the water environment. The results suggest that elevated temperatures, increased salinities, and the coexistence of fulvic acid promote the release of Nile blue. This simple and fast protocol overcomes the difficulties related to hotspot accessibility and detection repeatability for hydrophobic analytes, holding out extensive application prospects in environmental monitoring and chemical analysis.
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Journal of Materials Chemistry B
·
2024
·
IF 6.1
Caiyan Yuan,Shuangyan Chang,Chong Zhang,Dirong Dong,Jiahui Ding,Ali Reza Mahdavian,Zheng Hu,Lili Sun,Songwei Tan
Nasopharyngeal carcinoma (NPC) is one of the most common tumors in South China and Southeast Asia and is thought to be associated with Epstein-Barr virus (EBV) infection. Downregulation of latent membrane protein 1 (LMP1) encoded by EBV can reduce the expression of NF-κB and PI3K, induce apoptosis, and inhibit the growth of EBV-related NPC. To targeting cleave Lmp1 oncogene via the CRISPR/Cas9 gene editing system, a post-cross-linked ROS-responsive poly (β-amino ester) (PBAE) polymeric vector was designed for the delivery of CRISPR/Cas9 plasmids both in vitro and in vivo. After composition optimization, the resultant polymer-plasmid polyplex nanoparticles (NPs) showed a diameter of ~ 230 nm and zeta-potential of 22.3 mV with good stability. Compared with the non-cross-linked system, the cross-linked NPs exhibited efficient and quick cell uptake, higher transfection efficiency in EBV-positive C666-1 cells (53.5% vs. 40.6%), more efficient gene editing ability against the Mucin2 model gene (Muc2) (17.9% vs. 15.4%) and Lmp1 (8.5% vs. 5.6%), and lower intracellular reactive oxygen species (ROS) levels. The NPs achieved good tumor penetration and significant tumor growth inhibition in the C666-1 xenograft tumor model via Lmp1 cleavage, indicating their potential for gene therapy of EBV-related NPC.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
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IF 4.9
Zhaolei Zhang,Fengyan Fang,Pingping Zhang,Xiaokang Zhang,Hongchao Ma,Yanhui Wei
Efficient detection of 4- nitrophenol (4-NP) organic pollutant is always a very important issue due to the rapid development of chemical industry and the abuse of pesticides and so on. Here, we designed and synthesized a novel supramolecular fluorescent probe , 4-[4-(acetylamino)styryl]-1-cyclodextrin pyridinium p-toluenesulfonate (ASPT-β-CD), for the selective and sensitive detection of 4-NP. The introduction of β-CD not only improves the water solubility of probe and can also be used as a host to encapsulate 4-NP. In alkaline aqueous solution, the absorption spectrum of 4-NP overlaps the excitation spectrum of ASPT-β-CD and suggests that an internal filtration effect occurs between them. We explored the possible response mechanism and speculated that the high selectivity and high sensitivity were derived from the synergistic effect of inner filtering effect and host-guest interaction. Benefiting from the synergistic effect, the proposed sensor ASPT-β-CD displayed significant "turn-off" after adding 4-NP, while showing a fast response to 4-NP within 2 min and low LOD about 0.01 μM. In addition, this method has been successfully applied to the determination of 4-NP in lake water samples, indicating that the obtained ASPT-β-CD has great practical application potential in the environmental field.
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JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Wenyan Jiang,Shan Zhang,Chen Xu,Haojie Sun,Ziye Xing,Xiao Liang,Hongming He,Quanshun Li
The prevalence of antibiotics in natural aquatic environment has become an emerging class of organic pollutants, impacting both aquatic ecosystems and human health. Herein, spherical mesoporous covalent organic frameworks (SMCOF) was successfully synthesized and employed in the immobilization of OXA-48 β-lactamase through pore adsorption, in which 1,3,5-tris(4-aminophenyl)benzene and 2,5-dimethoxyterephthalaldehyde were utilized as the node and linker, respectively. Structural characterization demonstrated that OXA-48 was successfully adsorbed into the pores of SMCOF, achieving a loading capacity of 546.81 mg/g. Meanwhile, OXA-48@SMCOF maintained the catalytic activity of 62.08 % and 104.98 % after the treatment at 50 °C (pH of 7.4) and 37 °C (pH of 11) for 30 min, respectively. Further, all the catalytic activities of OXA-48@SMCOF remained above 88 % after the incubation in the presence of various organic solvents or proteinase K for 1 h. Moreover, OXA-48@SMCOF retained 53.78 % and 41.18 % of original activity after 15 cycles of reuse and 10 days of storage, respectively, indicating its favorable reusability and storage stability. In the application of degrading antibiotics in lake water samples, OXA-48@SMCOF demonstrated superior degradation performance compared to free OXA-48, attributing to the protective effect of SMCOF. Overall, the present study provides a promising technique to construct high performance immobilized enzymes, which is beneficial for the future application of OXA-48@SMCOF in the degradation of antibiotics during water treatment process.
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INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
·
2025
·
IF 2.5
Qiang Wang,Xinyi Chang,Yifan Wang,Qian Yu,Lingzi Xu,Xianming Kong
Dye wastewater pollutes aquatic ecosystems, has toxic effects on aquatic organisms and can enter the human body through the food chain, causing potential health hazards. Magnetic ferrite-based adsorbents have attracted a lot of attention because they combine the advantages of magnetic materials and adsorbent materials. In this study, the activated carbon (AC) was crosslinked with Fe3O4 nanoparticles and β-cyclodextrin polymer (β-CDP) by a simple method to obtain MAC/β-CDP biosorbent, which can effectively remove methylene blue (MB). The specific surface area of MAC/β-CDP was 434 m2 g−1 by nitrogen adsorption desorption method. The MAC/β-CDP composite shown good adsorption capacity. The optimal adsorption conditions were determined as follows: initial pH = 8, MAC/β-CDP dosage (0.2 g/50 mL), contact time (180 min) and the removal rate of cationic dye reached more than 97.14%. In addition, the maximum adsorption capacity of MAC/β-CDP composite for MB (50 mL) was 1.21 mg g−1, and the adsorption isotherm curve followed Langmuir (R2 = 0.9621) and Freundlich (R2 = 0.9982) models. In addition, after three cycles of regeneration, the regeneration rate of MAC/β-CDP was 96.76%. These results demonstrate the potential of MAC/β-CDP in the practical removal of MB pollutants in wastewater.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yanan Guo,Tianfu Cheng,Shuo Zhang,Haotian Liu,Ramnarain Ramakrishna,Lianzhou Jiang,Zhongjiang Wang,Zengwang Guo
The interaction between polysaccharides and proteins in co-adsorption played a critical role in determining interfacial properties and emulsion stability. This study focused on the regulation mechanism of different concentrations of polysaccharide on the dynamic adsorption behavior of protein, and how the interfacial film microstructure affected the stability of emulsion. The research demonstrated that Konjac Glucomannan (KGM) and β-conglycinin (7S) formed stable composite through hydrogen bonding and hydrophobic interactions. The results from interfacial adsorption kinetics and Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D) indicated that the structure of 7S stable interface layer is loose and disordered, which was not conducive to the formation of emulsion. KGM could synergistically facilitate the initial diffusion, penetration, and rearrangement of 7S at the oil-water interface thus forming a dense and viscoelastic three-dimensional gel-like multilayer interfacial structure. High-density adsorption of the 7S-KGM composite significantly increased the thickness and the protein content of the interfacial layer, enhancing emulsion stability. Dissipative particle dynamics simulations further demonstrated that the synergistic adsorption of KGM enhanced the interfacial adsorption efficiency of 7S, thereby reducing droplet size. This provided insights into the mechanisms by which polysaccharides enhanced protein emulsification performance, offering critical guidance for optimizing protein interfacial properties and enhancing emulsion stability.
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FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Jiayao Gan,Wenyu Jiang,Wei Liao,Huan Liu,Jielun Hu
The purpose of this research was to investigate the application of azobenzene modified polysaccharide on the single probiotic encapsulation by carbodiimide-mediated amidation and evaluate its targeted delivery potential in colon. Yeast β-glucan (YBG) was first modified by azobenzene groups via esterification (AA-YBG), which was then conjugated to the amino groups on the Escherichia coli Nissle 1917 (EcN) surface membrane through carbodiimide-mediated amidation, resulting in the successful fabrication of single probiotic microcapsules (EcN@AA-YBG). SEM and TEM showed that AA-YBG formed a nano-sacle intact thin film on the surface of EcN through covalent coupling between amino groups on the EcN membrane and the carboxyl groups in AA-YBG molecular chains as evidenced by FT-IR, and the biocompatibility analysis showed that AA-YBG coupled nanolayer has no significant effect on the proliferation of probiotics. In vitro gastrointestinal digestion showed that compared to the free EcN which suffered structural disintegration and lost all the viability within 0.5 h of gastric acid digestion, the EcN@AA-YBG maintained robust structural integrity with viability above 77% after the entire 6 h of digestion. Colonic targeted release analysis revealed that EcN@AA-YBG not only susceptible to enzymatic cleavage by colonic azo reductase and hypoxic colonic condition, triggering the rapid release of probiotics in colon, the adhesion of EcN@AA-YBG to colon mucosa was significantly improved as well. Furthermore, due to the compact and intact surface nanocoating of AA-YBG, the resistance of EcN@AA-YBG to high temperature, antibiotics and reactive oxygen species conditions were all improved significantly.
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WATER ENVIRONMENT RESEARCH
·
2026
·Vol. 98
·期 4
·e70380
·
IF 2.8
Shifeng Li,Yan Zhou,Shiyu Wang,Shuhua Yao,Min Xiao
Arsenic contamination in water, particularly from highly toxic arsenite (As(III)), continues to pose a significant global public health challenge due to its high mobility and resistance to conventional treatment methods. This study presents an innovative core-shell β-FeOOH@MnO 2 nanocomposite, which synergistically integrates MnO 2 nanosheets to oxidize As(III) into adsorptive arsenate (As(V)) and β-FeOOH to facilitate the efficient uptake of As(V). This approach addresses common limitations such as particle aggregation and suboptimal adsorption observed in pure MnO 2 systems. The nanocomposite, synthesized by hydrothermal methods, exhibits a doubled surface area of 172.95 m 2 /g, offering numerous active sites and mesopores conducive to rapid mass transfer. Under optimized conditions (pH 3.0, 0.4-g/L dosage), the nanocomposite achieved 98.39% removal of As(III) and a maximum adsorption capacity of 50.26 mg/g at 25°C, following pseudo-second-order kinetics and endothermic chemisorption. Characterization techniques, including X-ray diffraction (XRD), transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS), confirmed the synergistic mechanism, wherein MnO 2 oxidizes As(III) through the reduction of Mn 4+ to Mn 3+ , and the resulting As(V) is adsorbed by β-FeOOH. This advancement offers a cost-effective and efficient solution for improved arsenic remediation in water treatment applications. Graphical A core-shell β-FeOOH@MnO 2 nanocomposite effectively removes toxic arsenite by oxidizing mobile As(III) to adsorptive As(V) and adsorbing it, achieving high removal efficiency.
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