Frontiers in Cardiovascular Medicine
·
2022
·
IF 5.846
Hongge Fa,Dandan Xiao,Wenguang Chang,Lin Ding,Lanting Yang,Yu Wang,Mengyu Wang,Jianxun Wang
Objective Many studies have reported that microRNAs (miRs) are involved in the regulation of doxorubicin (DOX)-induced cardiotoxicity. MiR-194-5p has been reported significantly upregulated in patients with myocardial infarction; however, its role in myocardial diseases is still unclear. Various stimuluses can trigger the endoplasmic reticulum (ER) stress and it may activate the apoptosis signals eventually. This study aims to explore the regulatory role of miR-194-5p in DOX-induced ER stress and cardiomyocyte apoptosis. Methods H9c2 was treated with 2 μM DOX to induce apoptosis, which is to stimulate the DOX-induced cardiotoxicity model. The expression of miR-194-5p was detected by quantitative real-time PCR (qRT-PCR); the interaction between miR-194-5p and P21-activated kinase 2 (PAK2) was tested by dual luciferase reporter assay; terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay and caspase-3/7 activity were used to assess apoptosis; trypan blue staining was applied to measure cell death; Western blotting was performed to detect protein expressions; and ER-related factors splicing X-box binding protein 1 (XBP1s) was detected by polyacrylamide gel electrophoresis and immunofluorescence to verify the activation of ER stress. Results MiR-194-5p was upregulated in cardiomyocytes and mouse heart tissue with DOX treatment, while the protein level of PAK2 was downregulated. PAK2 was predicted as the target of miR-194-5p; hence, dual luciferase reporter assay indicated that miR-194-5p directly interacted with PAK2 and inhibited its expression. TUNEL assay, caspase-3/7 activity test, and trypan blue stain results showed that either inhibition of miR-194-5p or overexpression of PAK2 reduced DOX-induced cardiomyocyte apoptosis. Silencing of miR-194-5p also improved DOX-induced cardiac dysfunction. In addition, DOX could induce ER stress in H9c2, which led to XBP1 and caspase-12 activation. The expression level of XBP1s with DOX treatment increased first then decreased. Overexpression of XBP1s suppressed DOX-induced caspase-3/7 activity elevation as well as the expression of cleaved caspase-12, which protected cardiomyocyte from apoptosis. Additionally, the activation of XBP1s was regulated by miR-194-5p and PAK2. Conclusion Our findings revealed that silencing miR-194-5p could alleviate DOX-induced cardiotoxicity via PAK2 and XBP1s in vitro and in vivo . Thus, the novel miR-194-5p/PAK2/XBP1s axis might be the potential prevention/treatment targets for cancer patients receiving DOX treatment.
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BMJ Open Diabetes Research & Care
·
2023
·Vol. 11
·期 3
·e003282
·
IF 4.1
Lijuan Chen,Lin Guo,Susu Feng,Congcong Wang,Zhicheng Cui,Sijing Wang,Qingmiao Lu,Hang Chang,Bo Hang,Antoine M Snijders,Jian-Hua Mao,Yibing Lu,Dafa Ding
Introduction Gut microbiome (GM) deregulation has been implicated in major conditions such as obesity and type 2 diabetes (T2DM). Our previous prospective study indicated that fecal microbiota transplantation (FMT) successfully improved patients with T2DM. We hypothesized that FMT may be a potential therapeutic method for T2DM, but its precise mechanisms in T2DM remains to be elucidated. Research design and methods Eight db/m mice were FMT donors and control mice, and 16 genetically diabetic db/db mice were equally divided into two groups (db/db+phosphate-buffered saline (PBS) group, db/db+FMT group). The db/db+FMT group was administered fresh fecal suspension (0.2 mL/mice) daily for 4 weeks. Analysis of the GM and serum metabolome was carried out by 16S ribosomal RNA sequencing and liquid chromatogram-mass spectrometry, respectively. Effects of FMT on the gut barrier and pancreas were assessed using protein assays, messenger RNA, immunohistology and clinical indicators testing. Results Our results showed that FMT treatment of db/db mice relieves a series of clinical indicators, including fasting plasma glucose, serum insulin and oral glucose tolerance test among others. Compared with non-diabetic control mice, db/db+PBS mice exhibited decreased abundance of Ruminococaceae, Porphyromonadaceae and increased abundance of Rikenellaceae and Lactobacillaceae. FMT treatment reversed this effect on the microbiome. Eleven metabolites were changed between the db/db+PBS and db/db+FMT groups. Correlation analysis showed that the structural changes of the GM were correlated with host metabolite levels. We further showed that FMT treatment of db/db mice improved intestinal barrier function, reduced inflammation and caused an alteration in the number of circulating immune cells. Conclusions FMT-mediated changes in the GM, serum metabolites, intestinal epithelial barrier, inflammation and circulating immune cells play an important role in the efficacy of FMT on T2DM disease progression.
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JOURNAL OF MOLECULAR LIQUIDS
·
2023
·
IF 6
Hongbo Zhou,Yang Chen,Chao Luo,Honggun Song,Hong Yan,Lishibao Lin,Zhi Hu
The high-efficiency and non-toxic inhibitors for copper and its alloys are of great importance for reducing the corrosion rate of copper in aggressive solutions. In this work, the inhibition effect of 2-mercaptopyridine (2-MP) for copper in NaCl aqueous solution was investigated by experiment and theoretical calculation. The comprehensive experimental results showed that the surface of the copper sample was still smooth after 72 h immersion in NaCl solution containing 0.75 g/L 2-MP inhibitor, and the inhibition efficiency could reach 93.27 %. Meanwhile, the Cu N/S Cu bonds were detected by X-ray photoelectron spectroscopy and Raman spectroscopy, indicating that the 2-MP inhibitor could adsorb on the copper surface directly. The adsorption of copper by 2-MP is compatible with the Langmuir isotherm adsorption. The difference in reactivity between 2-MP and 4-MP was analyzed by quantum chemistry calculation, while molecular dynamics (MD) simulation results showed that 2-MP has better inhibition performance than 4-MP. Furthermore, the compactness and hydrophobicity of the 2-MP molecular layer were increased with a rise in 2-MP concentration, which effectively obstructed the diffusion of the water molecular and prevented the corrosive medium contact with the copper surface.
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MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Zhefei Hu,Yanhao Jiang,Panpan Cao,Yanjuan Liu,Yuefei Zhang,Wei Chen,Sheng Tang
In this work, 2-undecylimidazole (UI) and propyl methacrylate (PMA) dual-functionalized silica gel was prepared and applied as a mixed-mode chromatographic stationary phase. Successful preparation of this stationary phase (SiO 2 -UI-PMA) was proved through a range of characterization techniques. The chromatographic performance of the obtained SiO 2 -UI-PMA was investigated for the separation of environmental endocrine disruptors (phthalates, steroid hormones, bisphenols and alkylphenols), sulfonamides and food-borne stimulants in reversed-phase chromatography, as well as for the separation of inorganic anions in anion-exchange chromatography. The effect of column temperature, acetonitrile content, salt concentration and mobile phase pH on retention was studied to explore the separation mechanism, and the results indicated that this stationary phase provided multiple interactions with analytes. Compared with the commercial C18 column, the SiO 2 -UI-PMA demonstrated more preferable separation ability under the mobile phase conditions with a low content of acetonitrile, thereby acting as a good alternative candidate for the conventional reversed-phase stationary phase in chromatographic analysis. Furthermore, the SiO 2 -UI-PMA stationary phase exhibited high column efficiency (45 860 plates m −1 for progesterone) even though it was prepared from the non-serviceable silica based chromatographic packing materials.
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ACS Sustainable Chemistry & Engineering
·
2021
·
IF 8.198
Lungang Chen,Yong Liu,Canshuo Gu,Gang Feng,Xinghua Zhang,Jianguo Liu,Qi Zhang,Chenguang Wang,Longlong Ma
Selective production of 2-butanol from levulinic acid (LA) hydrogenolysis over a non-precious catalyst is long desirable. However, the process requires the precious metal Ru, and the yield of 2-butanol was less than 65%. Herein, we prepared Ni-based bimetallic catalysts, among which the NiMn catalyst exhibited the highest efficiency and reached an 84.5% yield of 2-butanol at 100% LA conversion under optimal conditions. The electronic states and surface properties were characterized by various techniques such as X-ray diffraction, X-ray photoelectron spectroscopy, temperature-programmed desorption, temperature-programmed reduction, and scanning electron microscopy/transmission electron microscopy. The high efficiency of the NiMn catalyst was due to the synergy between Ni0 and Mn2+ species, and the modified surface showed a high basicity and a high affinity to adsorb LA and LA-derived molecules with C═O or COOH. The high affinity of the NiMn catalyst was further confirmed by density functional theory calculations.
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BIOSENSORS & BIOELECTRONICS
·
2023
·
IF 12.6
Jiareng Chen,Willis Kwun Hei Ho,Bohan Yin,Qin Zhang,Chuanqi Li,Jiaxiang Yan,Yingying Huang,Jianhua Hao,Changqing Yi,Yu Zhang,Siu Hong Dexter Wong,Mo Yang
Upconversion nanoparticles (UCNPs) are ideal donors for luminescence resonance energy transfer (LRET)-based biosensors due to their excellent upconversion luminescence properties . However, the relatively large size of antibodies and proteins limits the application of UCNPs-based LRET biosensors in protein detection because the large steric hindrance of proteins leads to low energy transfer efficiency between UCNPs and receptors. Herein, we developed a magnetic responsive UCNPs-based LRET biosensor to control the coupling distance between antibody-functionalized UCNPs (Ab-UCNPs) as donors and antibody-PEG linker-magnetic gold nanoparticles (Ab-PEG-MGNs) as acceptors for ultrasensitive and highly selective detection of SARS-CoV-2 spike proteins . Our results showed that this platform reversibly shortened the coupling distance between UCNPs and MGNs and enhanced the LRET signal with a 10-fold increase in the limit of detection (LOD) from 20.6 pg/mL without magnetic modulation to 2.1 pg/mL with magnetic modulation within 1 h. The finite-difference time-domain (FDTD) simulation with cyclic distance change confirmed the distance-dependent LRET efficiency under magnetic modulation, which supported the experimental results. Moreover, the applications of this magnetic-responsive UCNP-based LRET biosensor could be extended to other large-size biomolecule detection.
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MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Mengying Luo,Yinnan Song,Niaz Ali Khan,Wanru Wei,Ying Lu,Qinghua Zhao,Haiqing Jiang,Mufang Li,Dong Wang
H 2 O 2 sensor is widely used in biomedicine, environmental governance and food processing. However, it is limited by the complex preparation processes and high cost of raw materials. In this work, we develop a facile, simple and cost-effective method to fabricate H 2 O 2 sensor based on a screen-printed electrode via in-situ growth method. The copper was first electrodeposited on the working electrode of screen-printed electrode (SPE) as a sacrificial layer, subsequently, the SPE was immersed in horseradish peroxidase (HRP) containing phosphate buffer saline (PBS). The Cu ions formed by oxidation of the copper layer combined with HRP and phosphate ions to construct HRP-Cu 3 (PO 4 ) 2 hybrid nanoflowers on its surface. The fabrication process is straightforward, without complex reaction conditions and dangerous drugs, which is conducive to mass production. This sensor showed a good linear response to H 2 O 2 with a detection limit of 1.8 μM and a sensitivity of 263.3 μA∙mM −1 ∙cm −2 . Additionally, it possessed excellent selectivity and long-time stability. The performance of this sensor was investigated in various beverages such as milk, beer, and juice, showing reliable detection of H 2 O 2 which highlights the potential practical applications of the sensor.
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ACS Applied Nano Materials
·
2023
·
IF 5.9
Junru Li,Yunqi Huang,Yanli Zhou,Hui Dong,Haoyuan Wang,Haoran Shan,Yifei Li,Maotian Xu,Xiaobing Wang
The conductive metal–organic framework (CMOF) is a promising application nanomaterial for the construction of electrochemical sensors. Three two-dimensional conductive M3(HHTP)2 materials with larger aspect ratios were prepared by a one-pot method using acetate-based metals (Cu, Ni, Co) and 2,3,6,7,10,11-hexahydroxytriphenylene (HHTP) with the introduction of N,N-dimethylformamide (DMF) as the limiting reagent. Compared with Ni3(HHTP)2 and Co3(HHTP)2, Cu3(HHTP)2 had a larger double capacitance, pore size, specific surface area, and smaller resistance. As a result, Cu3(HHTP)2 provided greater catalytic oxidation active surface area and charge transfer efficiency for malachite green (MG) detection. Hence, Cu3(HHTP)2 efficiently catalyzed MG oxidation and significantly improved the sensitivity of MG detection. Meanwhile, the oxidation current signal from the copper of Cu3(HHTP)2 was used as an internal reference signal to form a ratiometric electrochemical sensor with an MG oxidation current signal, which effectively reduced the external interference. The constructed electrochemical assay for MG detection displayed broad linearity (5–1000 nM) with a detection limit of 1.34 nM. In the meantime, MG residues in fish was evaluated and high reliability was obtained, confirming the feasibility of this valuable method for food safety and health evaluation of aquatic products.
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MOLECULAR PHARMACEUTICS
·
2019
·
IF 4.396
Jiaoyang Tao,Weidong Fei,Hongxia Tang,Chaoqun Li,Chaofeng Mu,Hongyue Zheng,Fanzhu Li,Zhihong Zhu
The poor capability of drugs to permeate through the blood–brain barrier (BBB) and further release inside glioma greatly limits the curative effects of glioma chemotherapies. In this study, we prepared angiopep-2-conjugated liposome-silica hybrid nanovehicles for targeted delivery and increased the permeation of arsenic trioxide (ATO) in glioma. Polyacrylic acid (PAA) was grafted on mesoporous silica nanoparticles (MSN) for pH-sensitive release and supporting the lipid membrane. The prepared “core–shell” nanovehicles (ANG-LP-PAA-MSN) were characterized with uniform size, high drug loading efficiency (8.19 ± 0.51%), and superior pH-sensitive release feature. From the experiments, the enhanced targeted delivery of ATO by ANG-LP-PAA-MSN (ANG-LP-PAA-MSN@ATO) was evidenced by the improvement of transport, enhanced cellular uptake, and apoptosis in vitro. In addition, the pharmacokinetic study was creatively carried out through the blood–glioma synchronous microdialysis and revealed that the half-life (t1/2) of blood and glioma tissue in the ANG-LP-PAA-MSN@ATO treatment group was extended by 1.65 and 2.34 times compared with the ATO solution group (ATO-Sol). The targeting efficiency of ANG-LP-PAA-MSN@ATO (24.96%) was dramatically stronger than that of the ATO-Sol (5.94%). Importantly, ANG-LP-PAA-MSN@ATO had a higher accumulation (4.6 ± 2.6% ID per g) in tumor tissues and showed a better therapeutic efficacy in intracranial C6 glioma bearing rats. Taken together, the blood–glioma synchronous microdialysis was successful used for the pharmacokinetic study and real-time monitoring of drug concentrations in blood and glioma; ANG-LP-PAA-MSN could be a promising targeted drug delivery system for glioma therapy.
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INORGANIC CHEMISTRY
·
2022
·
IF 5.436
Dan-Na Song,Wen-Yan Li,Hong-Yun Wang,Jing Zhang,Dao-Jun Zhang,Jun-Jie Wang,Ji-Min Du,Ren-Chun Zhang,Yong-Lin An
Cluster-based framework metal iodides have diverse structures and excellent luminescence properties, and show promising applications in sensing and solid-state lighting. However, the design and synthesis of these materials remain great challenges because excess I– ions introduced into the synthesis systems decrease the condensation degree of M–I units. In this work, a new strategy is developed to control the condensation behavior of Ag–I units, and a new silver-rich cluster-based framework iodide [DabcoAg8I6(SPh)2]n (1) (Dabco = 1,4-diazabicyclo [2.2.2] octane) has been synthesized under solvothermal conditions in the presence of silver thiophenolate (AgSPh)n. Compound 1 features a three-dimensional (3-D) cluster-based framework with a pillared layer structure composed of cationic [Ag8I6]2+ clusters bridged by SPh– and Dabco, and displays low-temperature dual emission and luminescence thermochromism.
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DALTON TRANSACTIONS
·
2023
·Vol. 52
·期 48
·18342-18353
·
IF 4
Minghao Dou,Mengjie Yao,Kai Ding,Yuye Cheng,Hongyu Shao,Shenjie Li,Yanyan Chen
Heterostructures formed by combining semiconductor materials with different band structures can provide work functions, d-band positions and electronic properties different from bulk materials and are considered as an effective strategy to improve the catalytic activity through electronic modification. In this study, an efficient MoS2/Fe-Ni3S2/NF heterojunction material was prepared by a two-step hydrothermal method. With the help of flake Ni(OH)2 synthesized in the first step, growth sites were provided for flake Ni3S2. The electronic structure of Ni3S2 was optimized by Fe doping, while the construction of the MoS2/Fe-Ni3S2 heterostructure allowed the catalyst to expose more active sites. MoS2/Fe-Ni3S2/NF exhibited a small charge transfer resistance and excellent electrocatalytic performance. At a current density of 10 mA cm−2, only low overpotentials of 148 mV and 118 mV were required for the oxygen precipitation reaction (OER) and hydrogen precipitation reaction (HER), respectively. Notably, when MoS2/Fe-Ni3S2/NF is used as the anode and cathode for overall hydrolysis, only 1.51 V is required to reach a current density of 10 mA cm−2, demonstrating its great potential for application in hydrolysis. This work provides a feasible idea for the rational construction of non-precious metal bifunctional electrocatalysts with excellent performance.
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BIOORGANIC & MEDICINAL CHEMISTRY
·
2022
·
IF 3.461
Ting Xu,Jie-Ru Meng,Wanqing Cheng,Jia-Zheng Liu,Junyan Chu,Qian Zhang,Nannan Ma,Li-Ping Bai,Yong Guo
Honokiol, isolated from a traditional Chinese medicine (TCM) Magnolia officinalis , is a biphenolic compound with several biological activities. To improve and broaden its biological activity, herein, two series of honokiol thioethers bearing 1,3,4-oxadiazole moieties were prepared and assessed for their α -glucosidase and SARS-CoV-2 entry inhibitory activities. Among all the honokiol thioethers, compound 7l exhibited the strongest α -glucosidase inhibitory effect with an IC 50 value of 18.9 ± 2.3 µM, which was superior to the reference drug acarbose (IC 50 = 24.4 ± 0.3 µM). Some interesting results of structure–activity relationships (SARs) have also been discussed. Enzyme kinetic study demonstrated that 7l was a noncompetitive α -glucosidase inhibitor, which was further supported by the results of molecular docking. Moreover, honokiol thioethers 7e , 9a , 9e , and 9r exhibited potent antiviral activity against SARS-CoV-2 pseudovirus entering into HEK-293 T-ACE2 h . Especially 9a displayed the strongest inhibitory activity against SARS-CoV-2 pseudovirus entry with an IC 50 value of 16.96 ± 2.45 μM, which was lower than the positive control Evans blue (21.98 ± 1.98 μM). Biolayer interferometry (BLI) binding and docking studies suggested that 9a and 9r may effectively block the binding of SARS-CoV-2 to the host ACE2 receptor through dual recognition of SARS-CoV-2 spike RBD and human ACE2. Additionally, the potent honokiol thioethers 7l , 9a , and 9r displayed relatively no cytotoxicity to normal cells (LO2). These findings will provide a theoretical basis for the discovery of honokiol derivatives as potential both α -glucosidase and SARS-CoV-2 entry inhibitors.
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