MOLECULAR NEUROBIOLOGY
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2024
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IF 4.6
Xu Yao,Liu Yongsheng,Wu Yan,Sun Jingshan,Lu Xiaocheng,Dai Kun,Zhang Yiting,Luo Chengliang,Zhang Jian
Early brain injury caused by subarachnoid hemorrhage (SAH) is associated with inflammatory response and ferroptosis. Curcumin alleviates neuroinflammation and oxidative stress by as yet unknown neuroprotective mechanisms. The objective of this study was to investigate the impact of curcumin on neuronal ferroptosis and microglia-induced neuroinflammation following SAH. By examining Nrf2/HO-1 expression levels and ferroptosis biomarkers expression both in vitro and in vivo, it was demonstrated that curcumin effectively suppressed ferroptosis in neurons after SAH through modulation of the Nrf2/HO-1 signaling pathway. Furthermore, by analyzing the expression levels of Nrf2, HO-1, p-p65, and inflammation-related genes, it was confirmed that curcumin could prevent the upregulation of pro-inflammatory factors following SAH by regulating the Nrf2/HO-1/NF-κB signaling pathway in microglia. The ability of curcumin to reduce neuronal damage and cerebral edemas after SAH in mice was validated using TUNEL staining, Nissl staining, and measurement of brain tissue water content. Additionally, through implementation of the modified Garcia test, open field test, and Y-maze test, it was established that curcumin ameliorated neurobehavioral impairments in mice post-SAH. Taken together, these data suggest that curcumin may offer a promising therapeutic approach for improving outcomes following SAH by concurrently attenuating neuronal ferroptosis and reducing neuroinflammation. Graphical Abstract
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Clinical Oral Investigations
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2024
·Vol. 28
·期 10
·1-14
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IF 3.1
Liu Runze,Liu Pei,Luo Yi,Fan Wei,Fan Bing
Objectives The mannose phosphotransferase system (Man-PTS) plays crucial roles in the adaptive metabolic activity of Enterococcus faecalis ( E. faecalis ) in adverse environments. The aim of this study was to evaluate the role of Man-PTS in the alkaline resistance of E. faecalis against calcium hydroxide (CH) and the effect of metformin (Met) on the alkaline resistance of E. faecalis to CH. Materials and methods The regulatory role of Man-PTS EII in the alkaline resistance of E. faecalis was firstly investigated using a wild-type highly alkaline-resistant E. faecalis XS 003, standard ATCC 29212 and Man-PTS EIID gene deficient (△ mptD ) and overexpressing (+ mptD ) strains of E. faecalis . RNA sequencing of Met-treated E. faecalis was performed to further validate the effect of Met on Man-PTS. The effect of Met on CH resistance of E. faecalis was verified by evaluating the survival, membrane potential and permeability, intracellular pH and ATP, and the expression of Man-PTS EII and membrane transporter-related genes of E. faecalis . The effect of Met on the ability of CH to remove E. faecalis biofilm on the dentin surface was also tested. The in vivo therapeutic effect of Met plus CH (CHM) was further investigated in a rat apical periodontitis model induced by E. faecalis XS 003. Results Man-PTS EII significantly promoted the survival ability of E. faecalis in CH and enhanced its resistance to CH. The inhibition of Man-PTS EII by Met resulted in reduced alkaline resistance of E. faecalis in the presence of CH, while also enhancing the antimicrobial properties of CH against E. faecalis biofilm on dentin. Additionally, Met plus CH showed the synergistically promoted intra-canal E. faecalis infection control and healing of periapical lesion in rats. Conclusions Met could significantly reduce the alkaline resistance of E. faecalis against CH through the modulation of Man-PTS EII, and improved the antibacterial effect of CH against E. faecalis infection both in vitro and in vivo. Clinical relevance Met could significantly enhance the ability of CH to control E. faecalis infection through reducing the alkaline resistance of E. faecalis .
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JOURNAL OF NANOBIOTECHNOLOGY
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2024
·Vol. 22
·期 1
·1-27
·
IF 10.6
Pu Chaoyu,Wang Yong,Xiang Honglin,He Jiangtao,Sun Qiyuan,Yong Yuan,Chen Lu,Jiang Ke,Yang Hanfeng,Li Yuling
Background In diabetic wounds, hyperglycemia-induced cytotoxicity and impaired immune microenvironment plasticity directly hinder the wound healing process. Regulation of the hyperglycemic microenvironment and remodeling of the immune microenvironment are crucial.Results Here, we developed a nanozymatic functionalized regenerative microenvironmental regulator (AHAMA/CS-GOx@Zn-POM) for the effective repair of diabetic wounds. This novel construct integrated an aldehyde and methacrylic anhydride-modified hyaluronic acid hydrogel (AHAMA) and chitosan nanoparticles (CS NPs) encapsulating zinc-based polymetallic oxonate nanozyme (Zn-POM) and glucose oxidase (GOx), facilitating a sustained release of release of both enzymes. The GOx catalyzed glucose to gluconic acid and (H₂O₂), thereby alleviating the effects of the hyperglycemic microenvironment on wound healing. Zn-POM exhibited catalase and superoxide dismutase activities to scavenge reactive oxygen species and H₂O₂, a by-product of glucose degradation. Additionally, Zn-POM induced M1 macrophage reprogramming to the M2 phenotype by inhibiting the MAPK/IL-17 signaling diminishing pro-inflammatory cytokines, and upregulating the expression of anti-inflammatory mediators, thus remodeling the immune microenvironment and enhancing angiogenesis and collagen regeneration within wounds. In a rat diabetic wound model, the application of AHAMA/CS-GOx@Zn-POM enhanced neovascularization and collagen deposition, accelerating the wound healing process.Conclusion sTherefore, the regenerative microenvironment regulator AHAMA/CS-GOx@Zn-POM can achieve the effective conversion of a pathological microenvironment to regenerative microenvironment through integrated control of the hyperglycemic-immune microenvironment, offering a novel strategy for the treatment of diabetic wounds.
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Nature Communications
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2024
·Vol. 15
·期 1
·1-9
·
IF 14.7
Yang Qing,Yang Xinyi,Wang Yixuan,Fei Yunfan,Li Fang,Zheng Haiyan,Li Kuo,Han Yibo,Hattori Takanori,Zhu Pinwen,Zhao Shuaiqiang,Fang Leiming,Hou Xuyuan,Liu Zhaodong,Yang Bing,Zou Bo
Luminescent materials that simultaneously embody bright singlet and triplet excitons hold great potential in optoelectronics, signage, and information encryption. However, achieving high-performance white-light emission is severely hampered by their inherent unbalanced contribution of fluorescence and phosphorescence. Herein, we address this challenge by pressure treatment engineering via the hydrogen bonding cooperativity effect to realize the mixture of n –π*/π–π* transitions, where the triplet state emission was boosted from 7% to 40% in isophthalic acid (IPA). A superior white-light emission based on hybrid fluorescence and phosphorescence was harvested in pressure-treated IPA, and the photoluminescence quantum yield was increased to 75% from the initial 19% (blue-light emission). In-situ high-pressure IR spectra, X-ray diffraction, and neutron diffraction reveal continuous strengthening of the hydrogen bonds with the increase of pressure. Furthermore, this enhanced hydrogen bond is retained down to the ambient conditions after pressure treatment, awarding the targeted IPA efficient intersystem crossing for balanced singlet/triplet excitons population and resulting in efficient white-light emission. This work not only proposes a route for brightening triplet states in organic small molecules, but also regulates the ratio of singlet and triplet excitons to construct high-performance white-light emission.
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MICROCHIMICA ACTA
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2019
·Vol. 187
·期 1
·1-9
·
IF 5.479
Lin Fangyuan,Lai Zhiwei,Zhang Linchun,Huang Yipeng,Li Feiming,Chen Pingyun,Wang Yiru,Chen Xi
Manganese(II)-doped zinc sulfide nanocrystals (Mn:ZnS NCs) with dual-emission fluorescence (peaks at 445 nm and 590 nm under 330 nm excitation), good water stability and low toxicity were synthesized by hot injection. The fluorescence intensity of both emission bands of the nanocrystals can change rapidly by the content of gaseous and dissolved oxygen. The process is fully reversible. Compared with the maximum intensity of Mn:ZnS sensing film in 100% nitrogen, the emission of the blue emission decreases by 72% in the presence of 100% oxygen, and the yellow emission by 32%. Response is linear in the presence of 3% to 12% of oxygen percentage in gas. For water-dissolved oxygen, the linear response occurs between 0.54 and 11.4 mg·L −1 .
Mn-doped ZnS NCs with dual-emission fluorescence were synthesized by hot-injection method. The reversible and rapid sensing characteristics of Mn-doped ZnS NCs to oxygen were studied, and the possible sensing mechanism was investigated.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-9
·
IF 15.7
Wu Yu,Li Jian,Jiang Wenxuan,Xu Weiqing,Zheng Lirong,Wang Canglong,Gu Wenling,Zhu Chengzhou
Early drug discovery is a time- and cost-consuming task requiring enzymes. Although nanozymes with metal sites akin to metallocofactors display similar activities, the lack of proximal amino acids hinders them from more adequately mimicking enzymes for drug discovery purposes. Hence, the rational design of the nanozyme second coordination sphere is desirable yet remains challenging. Herein, we report a nanozyme featuring atomically dispersed Cu-N 4 sites with proximal hydroxyl groups (CuNC-OH). Experimental and theoretical results reveal that Cu-N 4 site and hydroxyl respectively behave as cofactor and amino acid of the enzymatic pocket to interact with adsorbates, regulating nanozyme activity and inhibition. This mechanism involving dual sites is similar to that of thyroid peroxidases, which enables specific inhibition of CuNC-OH by antithyroid drugs. Based on these findings, a nanozyme-assisted drug discovery kit is established to analyze inhibition features of thyroid peroxidase inhibitors and screen out promising antithyroid drugs with a significant cost reduction compared with traditional enzyme kits.
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JOURNAL OF NANOBIOTECHNOLOGY
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2025
·Vol. 23
·期 1
·1-12
·
IF 12.6
Che Junyi,Huang Danqing,Wang Yang,Gao Guangtao,Zhao Yuanjin
Bioactive substance-integrated hydrogels have demonstrated efficacy in diabetic wound treatment. However, challenges remain in identifying naturally derived, multifunctional active substances capable of addressing the complex pathophysiology of wounds, as well as in tailoring hydrogels to enhance their suitability for wound applications. Here, we present a novel biological hydrogel microcarrier system by integrating Bletilla striata -derived nanoparticles (PdNPs) and polydopamine nanozymes (PDAs) into a hyaluronic acid-methacrylate (HAMA) hydrogel. PdNPs can polarize over-activated macrophages to an anti-inflammatory phenotype and restore fibroblast functionality. Meanwhile, PDAs act as potent reactive oxygen species (ROS) scavengers and protect fibroblasts from oxidative stress-induced apoptosis. When encapsulated into HAMA microcarriers, the PdNP + PDA@HAMA microcarriers significantly accelerate wound healing in a diabetic rat model. These outcomes demonstrate the therapeutic potential of our natural, multifunctional hydrogel microcarriers as a promising wound dressing platform for the treatment of chronic diabetic wounds.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
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2025
·Vol. 36
·期 2
·1-13
·
IF 2.8
Feng Hao,Li Xiaohua,Gao Shilun,Guo Ruijie,Wei Yuchen,Yang Dandan,Yang Huabin
With low redox potential, natural abundance and cost-effective of sodium resources, sodium-ion batteries (SIBs) are considered as a promising alternative for the currently dominant energy storage devices, i.e., lithium-ion batteries. However, developing suitable anode materials is still a challenge for the practical applications of SIBs. Alloy anodes have high specific capacity and low operating voltage, but the inherent volume expansion results in rapid capacity decay and poor cycling stability. Herein, focus on this issue, a novel carbon-coated alloy composite (SbSn@C) was synthesized by the solid-phase reduction of chloride method. Surface morphology analysis confirms that the SbSn@C composite exhibits a porous structure with a carbon layer of 20–30 nm, which can accommodate volume expansion. Furthermore, the synthesized SbSn@C composite exhibits impressive electrochemical performance with a good reversible capacity (450.5 mAh g −1 after 100 cycles, 90% capacity retention). As expected, such synthesis strategy provides a basis for the development of cost-effective and environmentally friendly sodium-ion batteries.
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Nature Communications
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2025
·Vol. 16
·期 1
·1-8
·
IF 15.7
Li Jidong,Zhao Wei,Fu Chenwei,Zhai Zhenpeng,Xu Pengfei,Diao Xinyuan,Guo Wanlin,Yin Jun
Biological nervous systems rely on distinct spiking frequencies across a wide range for perceiving, transmitting, processing, and executing information. Replicating this frequency range in an artificial neuron would facilitate the emulation of biosignal diversity but it remains challenging. Here, we develop an ion-electronic hybrid artificial neuron by compactly integrating a nonlinear electrochemical element with a solid-state memristor. This hybrid neuron employing a minimalist architecture exhibits a tunable spiking frequency spanning five orders of magnitude, significantly surpassing the capability of artificial neurons based on electronic devices. Notably, stimuli-dependent ion fluxes enable inherent afferent sensing of liquid flow, temperature, and chemical constituents, eliminating the need for separate, bulky sensors. Connection to biomotor nerves facilitates muscle actuation with frequency-regulated modes. The frequency encoding of a hybrid neuron array allows for the recognition of handwritten patterns. This hybrid neuron design, taking advantage of both ionic and electronic features, offers a promising approach for advanced e-skin and neurointerface technologies.
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BRITISH JOURNAL OF CANCER
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2025
·
IF 6.8
Chen Xiao,Cao Lixia,Feng Mengdan,Shuai Qiuying,Guo Min,Li Zhaoxian,Qi Chunchun,Shi Jie,Liu Yuxin,Zuo Siyu,Yu Tianwen,Hu Huayu,Wang Yanjing,Xue Yao,Sun Peiqing,Shi Yi,Wang Hang,Liu Lei,Yang Shuang
Background Breast cancer cells that break through the basement membrane interact directly with neighboring adipocytes. Therefore, adipocytes adjacent to the invasive front of tumour undergo lipolysis and transform into cancer-associated adipocytes (CAAs), which is vital for the malignant progression of breast cancer. However, tumor-derived factors that trigger this process are still to be determined. Methods Transcriptome sequencing was used to identify the downstream transcription factor of adrenomedullin (AM). Tet-On system was used to construct the 3T3-L1 cell line with inducible overexpression of Zeb1. Adipocyte-specific knock-in Zeb1 transgenic mice ( Zeb1 adiTG ) were used to construct an allograft tumor model. Results Breast cancer cell-derived AM downregulated the transcriptional expression of Zeb1 by triggering the cAMP/PKA/Creb1 pathway, thereby exerting lipolytic effects in CAAs. On the contrary, adipose tissue-specific upregulation of Zeb1 significantly attenuated AM-induced lipolytic phenotypes. Of note, we used the Zeb1 adiTG mice to construct allograft tumor models. The results confirmed that breast cancer cell-derived AM conferred tumorigenicity in vivo, which effect was predominantly dependent on the aberrant expression of adipocyte-specific Zeb1. Conclusions These findings collectively suggested that breast cancer cell-derived AM promotes lipid metabolic reprogramming through a Zeb1-dependent manner in CAAs, which displays significant clinical implications and may provide promising therapeutic approaches for targeting the breast cancer-associated adipose microenvironment.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-9
·
IF 15.7
Wang Xingyu,Fan Qi,Liu Ziheng,Zhu Xinyue,Yang Mei,Guo Zhiyuan,Chen Yuting,Wang Liuqi,Jing Yu,Xia Hui
The ether-based electrolytes are acknowledged for their compatibility with a diverse array of sodium-ion battery anodes, as well as their capability to enable efficient and reversible electrochemical reactions. However, they encounter a challenge of oxidation at high voltages. We find that a standard diglyme-based electrolyte starts to oxidize and break down at voltages exceeding 3.9 V (vs. Na + /Na). This deterioration is attributed to the nucleophilic nature of the diglyme solvent and the presence of oxygen atoms that possess two unpaired electrons. To address this issue, we incorporate foreign anions into the electrolyte system to passivate the reactive sites of terminal H on diglyme solvents, inhibiting further dehydrogenation and oxidation during battery operation. The constructed cathode electrolyte interphase, enriched with NaF and NaN x O y , substantially boosts the oxidation resistance of electrolyte to over 4.8 V (vs. Na + /Na), expanding the stability window and rendering it feasible for various high-voltage cathode materials. Our approach also ensures compatibility with either hard carbon or commercial graphite anodes, guaranteeing operation in pouch cells. This study elucidates the relationship between interfacial chemistry and oxidation tolerance at high voltages, offering an approach to the development of practical ether-based electrolytes for high-energy-density battery technologies.
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CATALYSIS LETTERS
·
2025
·Vol. 155
·期 7
·1-12
·
IF 2.4
An Li-Da,Li Feng,Qiu Yi-Feng,Liang Guo-Chao,Zhang Hao,Liu Hai Yang
Comprehending how substituent effect influences catalytic performance is essential for advancing efficient catalysts and unraveling reaction mechanisms. Examining the substituent effects of metal corrole catalyzed Baeyer–Villiger (B–V) oxidation reaction remains limited. In this work, four iron tri-aryl corrole complexes with distinct electronic structures, iron(IV) 5,10,15-tris(pentafluorophenyl) corrole [Fe IV (TPFC)Cl] ( 1 a), iron(IV) 5,15-bis(pentafluorophenyl)-10-phenyl corrole [Fe IV (BPFPC)Cl] ( 2 a), iron(IV) 5,15-bis(phenyl)-10-pentafluorophenyl corrole [Fe IV (BPPFC)Cl] ( 3 a) and iron(IV) 5,10,15-tris(phenyl) corrole [Fe IV (TPC)Cl] ( 4 a) have been prepared. The impact of electron-withdrawing meso-substituents on their catalytic performance in B-V oxidation reaction was then investigated. The results reveal that an increased number of pentafluorophenyl substituents, which enhance the electron-withdrawing effect, correlates with superior catalytic activity. The catalytic activity follows an order 1 a > 2 a > 3 a > 4 a. The plausible mechanism to explain the observed catalytic behavior was also discussed. Graphical
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