BIOELECTROCHEMISTRY
·
2019
·
IF 4.474
Shuan Zhang,Chunyan Zhang,Yilei Jia,Xiaobo Zhang,Yunhui Dong,Xinjin Li,Qing Liu,Yueyun Li,Zengdian Zhao
A sandwich-type electrochemical immunosensor was fabricated for the quantitation of alpha fetoprotein (AFP). To this end, the Au@Pt dendritic nanorods loaded with amino functionalized molybdenum selenide nanosheets (Au@Pt 41 DNRs/NH2-MoSe2 NSs) with enhanced peroxidase-like properties were selected as the secondary antibody label (Ab2) to achieve signal amplification. The as-obtained Au@Pt DNRs/NH2-MoSe2 NSs exhibited better catalytic activity toward hydrogen peroxide reduction and offered rich anchors for bioconjugation . Meanwhile, gold nanoparticles anchored on an amino functionalized graphene (Au NPs/NH2-GS) composite with admirable conductivity and biocompatibility was used as the matrix material to improve sensitivity. Under optimal conditions, amperometric current responses had a good linear relationship with the logarithm values of AFP concentration in the range 10 fg mL –1 to 200 ng mL –1 with a detection limit of 3.3 fg mL –1 (S/N = 3). Additionally, the immunosensor had excellent reproducibility, selectivity, and stability, which indicated superior performance for AFP detection compared with previous reports. The satisfactory results of human serum samples analysis showed that the designed immunosensor has potential applicability for the sensitive detection of other tumor markers.
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高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 12
·1-11
·
IF 5.833
Wang Xiao,Liao Xiaochen,Zhang Bingjian,Zhang Mengmeng,Mei Lisha,Wang Fangwai,Chen Siyu,Qiao Xiuwen,Hong Chenglin
Abstract Carcinoembryonic antigen levels in the human body reflect the conditions associated with a variety of tumors and can be used for the identification, development, monitoring, and prognosis of lung cancer, colorectal cancer, and breast cancer. In this study, an amperometric immunosensor with CuCo/carbon nanocubes (CuCo/CNC) as the signal label is constructed. The bimetal-doped carbon skeleton structure has a high specific surface area and exhibits good electrocatalytic activity. In addition, Au/g-C 3 N 4 nanosheets (Au/g-C 3 N 4 NSs) are used to modify the substrate platform, facilitating the loading of more capture antibodies. The reaction mechanism was explored through electrochemical methods, X-ray powder diffraction, X-ray photoelectron spectroscopy, and other methods. Kinetic studies have shown that CuCo/CNC have good peroxidase-like activity. In addition, the electrocatalytic reduction ability of CuCo/CNC on hydrogen peroxide can be monitored using amperometric i – t curve (− 0.2 V, vs. SCE), and the response current value is positively correlated with the CEA antigen concentration. The prepared electrochemical immunosensor has good selectivity, precision, and stability. The dynamic range of the sensor was 0.0001–80 ng/mL, and the detection limit was 0.031 pg/mL. In addition, the recovery and relative standard deviation in real serum samples were 97.7–103 % and 3.25–4.13 %, respectively. The results show that the sensor has good analytical capabilities and can provide a new method for the clinical monitoring of CEA. Graphic abstract
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2017
·
IF 6.216
Ziyin Yang,Xiaohui Zheng,Jianbin Zheng
A novel strategy is developed to synthesize three-dimensional porous Au@Pt core-shell nanoflowers supported on GO (Au@Pt-nFs/GO) for enhanced electrochemical sensing of hydrazine (N 2 H 4 ). Au@Pt-nFs/GO nanocomposite was synthesized through a facile and green method, where ascorbic acid and chitosan were employed as the reductant and linking agent, respectively. The experiment results revealed that a large numbers of Au@Pt-nFs with Au cores and Pt dendritic shells were distributed on the surface of GO sheets. Meanwhile, Au@Pt-nFs/GO with large surface area, abundant active sites and open structure reduced the overpotential and accelerated kinetics of N 2 H 4 oxidation, therefore making the sensor based on Au@Pt-nFs/GO exhibit an excellent performance for N 2 H 4 analysis with a wide linear range of 0.8 μM–0.429 mM, a high sensitivity of 1695.3 μA mM −1 cm −2 and a low detection limit of 0.43 μM (S/N = 3). Moreover, the sensor also exhibited good selectivity, repeatability and stability. Application of the sensor for the detection of N 2 H 4 in tap water samples was demonstrated.
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高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2025
·Vol. 172
·期 4
·047502
·
IF 3.3
Lanyue Wang,Beibei Wang,Kai Kang,Xueping Ji,Zhenzhen Zhao,Congwei Li,Yuping Wang
An electrochemical sensor utilizing Au-Ni@ etched metal-organic framework (etched MOF) on a glassy carbon electrode (GCE) was constructed for ultrasensitive detection of 3-nitrophenol (3-NP). The Au-Ni@etched MOF in situ nanocomposite was synthesized via a simple one-step process that combines the electrochemical etching of Ni-hemin MOF with the simultaneous electrodeposition of Au-Ni bimetallic nanoparticles. The etched MOF not only provides abundant active sites for adsorbing 3-NP, but also serves as a supporter for loading Au-Ni bimetallic nanoparticles. This innovative design endows the electrochemical sensor with exceptional electrocatalytic activity towards 3-NP, achieving a low detection limit of 0.015 μM over the ranges of 0.06 ∼ 10 μM and 10 ∼ 200 μM. Moreover, the electrochemical sensor also shows excellent sensitivity, repeatability, and stability in 3-NP detection. This method offers a highly sensitive electrochemical strategy for accurately assaying 3-NP in water samples and opens new avenues for the application of MOF derivatives in electrochemical platforms.
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Xuemin Zhong,Peng Yuan,Yanfu Wei,Dong Liu,Dusan Losic,Mengyuan Li
The aerobic oxidation of 5-hydroxymethylfurfural (HMF), a key platform compound derived from biomass, to 2,5-furandicarboxylic acid (FDCA) is a highly important reaction in the production of green and sustainable chemicals. Here, we developed a highly efficient and stable halloysite-supported Pt–Au alloy catalyst for the selective oxidation of HMF to FDCA. The catalyst was synthesized through the organosilane functionalization of halloysite nanotubes, followed by the in situ formation and dispersion of Pt–Au alloy nanoparticles on the internal and external surfaces of nanotubes. The composition, morphology, and structure of the prepared catalyst were characterized. The catalyst with the optimal composition of Pt/Au molar ratio of 1/4 and metal loading of 1.5 wt % exhibited outstanding catalytic activity for the oxidation of HMF to FDCA using O2 as an oxidant with 100% conversion of HMF and 99% selectivity of FDCA. This excellent catalytic performance is mainly attributed to the high dispersion and alloying effects of bimetallic nanoparticles, which promoted the activation of reactants or intermediates and further improved FDCA selectivity. Furthermore, the halloysite-supported Pt/Au bimetallic catalyst showed high stability and reusability. This study provides a promising strategy by combining clay mineral halloysite and bimetallic alloys for developing efficient catalysts with high FDCA selectivity and stability for the oxidation of HMF to FDCA.
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PubMed
高影响因子
Biosensors-Basel
·
2022
·Vol. 13
·期 1
·7
·
IF 5.743
Huiping Fu,Ning Ding,Dan Ma,Qing Xu,Bingyong Lin,Bin Qiu,Zhenyu Lin,Longhua Guo
In this work, a simple, low-cost, green, and mild method for the preparation of three-dimensional nanocomposite materials of gold nanorods (Au NRs)@TiO2is reported. The surface of Au NRs was coated with TiO2in situ reduction at room temperature without a complicated operation. The synthetic Au NRs@TiO2nanocomposites were used as surface-enhanced Raman spectroscopy (SERS) active substrates for the reusable and sensitive detection of environmental pollutants. The results showed that the pollutants on Au NRs@TiO2nanocomposites have higher SERS activity and reproducibility than those on the Au NR substrate without the presence of TiO2. Moreover, the SERS substrate can be readily recycled by UV-assisted self-cleaning to remove residual analyte molecules. Malachite green (MG) and crystal violet (CV) were used as examples to demonstrate the feasibility of the proposed sensor for the sensitive detection of environmental pollutants. The results showed that the limit of detections (LODs) were 0.75 μg/L and 0.50 μg/L for MG and CV, respectively, with the recoveries ranging from 86.67% to 91.20% and 83.70% to 89.00%. Meanwhile, the SERS substrate can be easily regenerated by UV light irradiation. Our investigation revealed that within three cycles, the Au NRs@TiO2substrates still maintained the high SERS enhancement effect that they showed when first used for SERS detection. These results indicated that the method can be used to detect MG and CV in really complex samples. Due to the high sensitivity, reusability, and portability and the rapid detection property of the proposed sensor, it can have potential applications in the on-site detection of environmental pollutants in a complex sample matrix.Keywords:surface-enhanced Raman spectroscopy (SERS);self-cleaning;photocatalytic activity;recyclable;environmental pollutants
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PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Zhiqing Cui,Weikang Wang,Cuijiao Zhao,Chun Chen,Miaomiao Han,Guozhong Wang,Yunxia Zhang,Haimin Zhang,Huijun Zhao
We present the self-assembly synthesis of core–shell structure Au/CeO2 composites with different Au loadings through a spontaneous chemical redox approach at an ambient temperature utilizing HAuCl4 and Ce(NO3)3 as reaction substrates in an alkaline environment. The results demonstrate that the as-synthesized Au/CeO2 composites exhibit spherical shape morphologies with porous structures, composed of Au nanoparticle (∼10 nm) cores and CeO2 nanoparticle shells with abundant oxygen vacancies. The introduction of Au nanoparticles in CeO2 not only effectively improves the visible light utilization efficiency but also provides rich surface catalytic active sites for highly efficient visible light photocatalysis. As visible light photocatalysts (λ > 400 nm), the as-synthesized Au/CeO2 composites with the Au loading amount ≥4.0 wt % exhibit high conversion and selectivity (∼100%) of benzyl alcohol to benzaldehyde under the given experimental conditions. Moreover, Au/CeO2 also shows a general applicability as a visible light photocatalyst for the selective oxidation of other alcohols to corresponding aldehydes or ketones. The photocatalytic mechanism studies indicate that the photoelectrons/holes produced from the photoexcited Au and the formed superoxide radicals in the oxygen vacancies of CeO2 synergistically contribute to the high performance of the selective photocatalytic oxidation of alcohols to aldehydes or ketones.
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PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Sha Liu,Siyu Lu,Shihao Sun,Jun Hai,Genping Meng,Baodui Wang
Quantitative detection of cancer cells using portable devices is promising for the development of simple, fast, and point-of-care cancer diagnostic techniques. However, how to further amplify the detection signal to improve the sensitivity and accuracy of detecting cancer cells by portable devices remains a challenge. To solve the problem, we, for the first time, synthesized folic-acid-conjugated Au nanoframes (FA-Au NFs) with amplification of pressure and temperature signals for highly sensitive and accurate detection of cancer cells by portable pressure meters and thermometers. The resulting Au NFs exhibit excellent near-infrared (NIR) photothermal performance and catalase activity, which can promote the decomposition of NH4HCO3 and H2O2 to generate corresponding gases (CO2, NH3, and O2), thereby synergistically amplifying pressure signals in a closed reaction vessel. At the same time, Au NFs with excellent peroxidase-like activity can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to produce TMB oxide (oxTMB) with a strong photothermal effect, thereby cooperating with Au NFs to amplify the photothermal signal. In the presence of cancer cells with overexpressing folate receptors (FRs), the molecular recognition signals between FA and FR can be converted into amplified pressure and temperature signals, which can be easily read by portable pressure meters and thermometers, respectively. The detection limits for cancer cells using pressure meters and thermometers are 6 and 5 cells/mL, respectively, which are better than other reported methods. Moreover, such Au NFs can improve tumor hypoxia by catalyzing the decomposition of H2O2 to produce O2 and perform photothermal therapy of cancer. Together, our work provides new insight into the application of Au NFs to develop a dual-signal sensing platform with amplification of pressure and temperature signals for portable and ultrasensitive detection of cancer cells as well as personalized cancer therapy.
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PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2013
·
IF 5.695
Shao-Kai Sun,Lu-Xi Dong,Yang Cao,Hao-Ran Sun,Xiu-Ping Yan
Facile fabrication of multimodal imaging probes is highly desired for bioimaging application due to their integrated advantages of several imaging modalities. Here, we report a simple and one-step mild strategy to fabricate a multifunctional Gd2O3/Au hybrid nanoprobe. Bovine serum albumin (BSA) was used as the template in the biomineralization synthesis. The fabricated BSA-Gd2O3/Au nanoprobe showed excellent chemical stability, intense near-infrared (NIR) fluorescence, and good magnetic resonance imaging (MRI) ability. The multimodal imaging potential of the prepared multifunctional nanoprobe was demonstrated by successful NIR fluorescent and magnetic resonance blood pool imaging. Further modification of BSA-Gd2O3/Au with arginine–glycine–aspartic acid peptide c(RGDyK) (RGD) enabled the nanoprobe for targeted tumor imaging in vivo.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Mingdang Li,Ping Wang,Fubin Pei,Haoxuan Yu,Yunhui Dong,Yueyun Li,Qing Liu,Peng Chen
In this work, the gold nanoparticles (Au NPs) substrate and the prepared mesoporous trimetallic PdPtCu nanospheres (m-PdPtCu) as the label of secondary antibodies (Ab 2 , Ab 2 labeled probe) were used as the basis for fabricating a highly sensitive immunosensor to detect trace amount of prostate specific antigen (PSA) in human serum. The Au NPs can provide a suitable microenvironment for immobilizing primary antibodies (Ab 1 ) and accelerate the electron transfer on the electrode interface due to the good biocompatibility and conductivity. The sensitivity of immunosensor was greatly amplified by the designed novel Ab 2 labeled probe, which can be attributed to the m-PdPtCu with mesoporous structure not only can provide high specific surface area, but also offer abundant catalytically active sites and accessible pores for reactants. Under the optimal conditions, a linear relationship between current responses and the logarithm of the PSA concentrations were obtained in the wide range of 10 fg mL −1 –100 ng mL −1 and the detection limit was 3.3 fg mL −1 . Remarkably, the detection result of PSA in real human serum samples by the immunosensor is well in correlation with commercial ELISA, which confirms that the immunosensor is promising in early clinical detection of biomarkers.
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高影响因子
GREEN CHEMISTRY
·
2022
·Vol. 24
·期 22
·8840-8852
·
IF 11.034
Zongyang Liu,Yuan Tan,Jie Li,Xiuli Li,Yan Xiao,Juan Su,Xingkun Chen,Botao Qiao,Yunjie Ding
Aerobic oxidation of 5-hydroxymethylfurfural (HMF) to 2,5-furandicarboxylic acid (FDCA) is a promising alternative route to produce renewable biodegradable plastics, which has stimulated intense interest in recent years. Supported gold catalysts have shown great potential for this reaction, but the selectivity and stability need to be improved. In addition, the requirement for base additives makes the process less effective and sustainable. Herein, highly active and selective Au–Ag bimetallic nanoclusters supported on MgAl-hydrotalcite (HT) were developed for HMF oxidation to FDCA under base-free reaction conditions using molecular oxygen as the oxidant. The presence of small amounts of Ag (0.034–0.065 wt%) in Au25 clusters greatly enhanced the catalytic reaction rates and selectivity for HMF tandem oxidative steps, leading to both high conversion of HMF (>97%) and high yield of FDCA (>95%). Besides, the catalyst stability was also enhanced by the addition of Ag. The characterization results suggested that the substitution of Ag atoms modified the electronic structure of Au while likely maintaining the primary geometry. Thus, a significant synergistic effect could be observed over trace Ag modified Au–Ag bimetallic nanoclusters. The present work provides new insight into the alloying effect, and helps understand highly efficient Au-based catalysts for aerobic oxidation of biomass derived molecules under base-free conditions.
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高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Haolin Xiao,Shanshan Wei,Miao Gu,Zhencheng Chen,Liangli Cao
Alpha-fetoprotein (AFP) plays an important role as a marker in the clinical diagnosis of cancer. However, the sensitive detection of AFP in clinical samples is still challenging. Therefore, we developed a highly sensitive electrochemical immunosensor for the effective detection of AFP. In this study, thionine (Thi) and gold nanoparticles (AuNPs) co-modified reduced graphene oxide-tetraethylene pentamine (rGO-TEPA-Thi-Au), which was used as the immunosensor platform to fix the primary antibody (Ab 1 ), leading to a large improvement in the sensitivity of the immunosensor. Furthermore, ordered mesoporous carbon (CMK-3) modified with AuPt binary nanoparticles ( [email protected] ) was used as the signal probe for further signal amplification because CMK-3 can provide a large specific surface area for in-situ reduction of AuPtNPs with good conductivity. Therefore, a dual signal amplification was observed in the amperometric i-t curve, and the detection limit of the proposed immunosensor was effectively enhanced. The constructed immunosensor showed a wide linear range of 0.005 to 100 ng mL −1 and a low detection limit of 0.0022 ng mL −1 at S/N = 3. Furthermore, the proposed immunosensor exhibited excellent stability and recovery, demonstrating its potential for clinical applications.
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高影响因子
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