BIOMATERIALS
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2016
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IF 8.387
Zi-Wei Jing,Yi-Yang Jia,Ning Wan,Min Luo,Meng-Lei Huan,Tai-Bin Kang,Si-Yuan Zhou,Bang-Le Zhang
The covalently modified ureido-conjugated chitosan/TPP multifunctional nanoparticles have been developed as targeted nanomedicine delivery system for eradication of Helicobacter pylori . H. pylori can specifically express the urea transport protein on its membrane to transport urea into cytoplasm for urease to produce ammonia, which protects the bacterium in the acid milieu of stomach. The clinical applicability of topical antimicrobial agent is needed to eradicate H. pylori in the infected fundal area. In this study, we designed and synthesized two ureido-conjugated chitosan derivatives UCCs-1 and UCCs-2 for preparation of multifunctional nanoparticles. The process was optimized in order to prepare UCCs/TPP nanoparticles for encapsulation of amoxicillin. The results showed that the amoxicillin-UCCs/TPP nanoparticles exhibited favorable pH-sensitive characteristics, which could procrastinate the release of amoxicillin at gastric acids and enable the drug to deliver and target to H. pylori at its survival region effectively. Compared with unmodified amoxicillin-chitosan/TPP nanoparticles, a more specific and effective H. pylori growth inhibition was observed for amoxicillin-UCCs/TPP nanoparticles. Drug uptake analysis tested by flow cytometry and confocal laser scanning microscopy verified that the uptake of FITC-UCCs-2/TPP nanoparticles was associated with urea transport protein on the membrane of H. pylori and reduced with the addition of urea as competitive transport substrate. These findings suggest that the multifunctional amoxicillin-loaded nanoparticles have great potential for effective therapy of H. pylori infection. They may also serve as pharmacologically effective nanocarriers for oral targeted delivery of other therapeutic drugs to treat H. pylori .
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Materials Science & Engineering C-Materials for Biological Applications
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2016
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IF 3.42
Xuan Liu,Yunfei Mo,Xiaoyu Liu,Rui Guo,Yi Zhang,Wei Xue,Yuanming Zhang,Changyong Wang,Seeram Ramakrishna
The development of safe and efficient gene carriers is the key to the clinical success of gene therapy. In the present study, carboxymethyl chitosan (CMCS) was prepared by chitosan (CS) alkalisation and carboxymethylation reactions. Then polyethyleneimine (PEI) was grafted to the backbone of CMCS by an amidation reaction. The CMCS–PEI copolymer showed strong complexation capability with DNA to form nanoparticles, and achieved lower cytotoxicity and higher transfection efficiency compared with PEI (25 kDa) towards 293T and 3T3 cells. Moreover, the haemocompatibility of the CMCS–PEI copolymer was investigated through the aggregation, morphology and lysis of human red blood cells (RBCs), along with the impact on the clotting function with activated partial thromboplastin time (APTT), prothrombin time (PT) and thromboelastographic (TEG) assays. The results demonstrated that the CMCS–PEI copolymer with a concentration lower than 0.05 mg/mL had little impact on the aggregation, morphology or lysis of RBCs, or on blood coagulation. Therefore, the copolymer may be a strong alternative candidate as an effective and safe non-viral vector.
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Analytical Methods
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2017
·Vol. 9
·期 35
·5149-5155
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IF 1.9
Zhenliang Li,Zunli Mo,Ruibin Guo,Shujuan Meng,Ruijuan Wang,Huhu Gao,Xiaohui Niu
A novel chiral electrochemical sensor based on hydroxyethyl chitosan (HECS) covalently binding with the carboxylic multi-walled carbon nanotubes (MWCNT–COOH) was fabricated for discrimination of tryptophan (Trp) enantiomers. HECS preferably combined with L-Trp than with D-Trp because of the favorability of host–guest interactions between HECS and Trp enantiomers. Thus, the recognition efficiency was remarkably improved after hydroxyethyl groups were grafted onto chitosan (CS); this might be attributed to the formation of hydrogen bonds between Trp enantiomers and HECS. In addition, the small stereo-hindrance effect could play an important role in enantioselective discrimination.
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Nuclear Engineering and Technology
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2017
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IF 1.144
Shuting Zhuang,Yanan Yin,Jianlong Wang
Chitosan was modified by gamma radiation–induced grafting with maleic acid and then used for the removal of cobalt ions from aqueous solutions. Chitosan-g-maleic acid was characterized by Fourier Transform infrared spectroscopy (FT-IR). The effect of the dose (1–5 kGy) and monomer concentration (0.3–1.3%, m/v) on the grafting ratio was examined. The adsorption kinetics and isotherms were also investigated. The results showed that the optimal dose for grafting was 2 kGy. When monomer concentration was within the range of 0.3–1.3% (m/v), the grafting ratio increased almost linearly. For the adsorption of cobalt ions by chitosan-g-maleic acid beads, the pseudo second-order kinetic model (R 2 = 0.99) and Temkin isotherm model (R 2 = 0.96) were able to fit the experimental data reasonably well. The equilibrium adsorption capacity of cobalt ions increased from 2.00 mg/g to 2.78 mg/g after chitosan modification.
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Science Bulletin
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2016
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IF 0
Miao He,Liying Lu,Jinchi Zhang,Danzhen Li
Highly stable dispersions of nanosized copper (Cu) particles with an average size of (2.6 ± 0.5) nm were synthesized by in situ reduction of Cu(II) to immobilize Cu nanoparticles on the amino-enriched surface of chitosan (CTS). The synthetic process and stability of the l -ascorbic acid-stabilized Cu-CTS nanocomposites were investigated by X-ray photoelectron spectroscopy and Fourier transform Infrared spectroscopy. The antimicrobial efficiency and potency of the Cu-CTS nanocomposites were studied. The Cu-CTS nanocomposites were found to exhibit a broad antimicrobial spectrum and high antimicrobial activity against Gram-positive bacterial pathogen Staphylococcus aureus and fungal pathogen Monilia albican . The minimum inhibitory concentration of the Cu-CTS nanocomposites toward S. aureus was found to be 6.4 μg mL −1 , much lower than those reported in the literature. Furthermore, the Cu-CTS nanocomposites were stable and maintained good disinfection potential even after 90-day shelf-time under ambient conditions.
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Materials Science & Engineering C-Materials for Biological Applications
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2015
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IF 3.088
Qingqing Yao,Wei Li,Shanshan Yu,Liwei Ma,Dayong Jin,Aldo R. Boccaccini,Yong Liu
Novel chitosan–polyvinyl pyrrolidone/45S5 Bioglass® (CS-PVP/BG) scaffolds were prepared via foam replication and chemical cross-linking techniques. The pristine BG, CS-PVP coated BG and genipin cross-linked CS-PVP/BG (G-CS-PVP/BG) scaffolds were synthesized and characterized in terms of chemical composition, physical structure and morphology respectively. Resistance to enzymatic degradation of the scaffold is improved significantly with the use of genipin cross-linked CS-PVP. The bio-effects of scaffolds on MC3T3-E1 osteoblast-like cells were evaluated by studying cell viability, adhesion and proliferation. The CCK-8 assay shows that cell viability on the resulting G-CS-PVP/BG scaffold is improved obviously after cross-linking of genipin. Cell skeleton images exhibit that well-stretched F-actin bundles are obtained on the G-CS-PVP/BG scaffold. SEM results present significant improvement on the cell adhesion and proliferation for cells cultured on the G-CS-PVP/BG scaffold. The drug release performance on the as-synthesized scaffold was studied in a phosphate buffered saline (PBS) solution. Vancomycin is found to be released in burst fashion within 24 h from the pristine BG scaffold, however, the release period from the G-CS-PVP/BG scaffold is enhanced to 7 days, indicating improved drug release properties of the G-CS-PVP/BG scaffold. Our results suggest that the G-CS-PVP/BG scaffolds possess promising physicochemical properties, sustained drug release capability and good biocompatibility for MC3T3-E1 cells' proliferation and adhesion, suggesting their potential applications in areas such as MC3T3-E1 cell stimulation and bone tissue engineering.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2015
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IF 2.858
Bitao Lu,Tianyou Wang,Zhiquan Li,Fangying Dai,Lingmei Lv,Fengling Tang,Kun Yu,Jiawei Liu,Guangqian Lan
A chitosan–gelatin sponge (CSGT) was prepared using a chitosan/ascorbic acid solution blend containing gelatin, followed by crosslinking with tannin acid and freeze-drying, thereby combining the chitosan sponge and gelatin sponge. The structure of the CSGT was observed by scanning electron microscopy and was shown to have uniform and abundant pores measuring about 145–240 μm in size. We also characterized the sponges by infrared spectroscopy, thermogravimetric analysis, mechanical property tests, swelling behavior analysis, water retention capacity tests, antibacterial property analysis, and cytotoxicity tests. Our data showed that the CSGT had good thermostability and mechanical properties as well as efficient water absorption and retention capacities. Moreover, the CSGT could effectively inhibit the growth of Escherichia coli and Staphylococcus aureus with low toxicity. In animal experiments, macroscopic observations and histological examinations showed that the wound covered by the CSGT healed quickly. Additionally, loading of the CSGT with platelet-rich plasma resulted in further acceleration of wound healing. Therefore, the CSGT and the CSGT with platelet-rich plasma were suitable for application as a wound dressing and may have potential for use in various biomedical applications.
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RSC Advances
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2015
·Vol. 5
·期 120
·99418-99424
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IF 3.84
Jinda Fang,Ke Zhang,Jingwei Jia,Zhengke Wang,Qiaoling Hu
To improve the drug-loading capacity and control the initial release of amphiphilic drug carriers, a series of N-phthaloyl-chitosan-g-(PEO–PLA–PEO) compounds were synthesized with well-defined structures. The self-assembly behavior of copolymers in aqueous solutions was confirmed by various techniques such as fluorescence spectrometry, dynamic light scattering, and transmission electron microscopy. The results demonstrated that the micellization behavior of graft copolymers was different from that of their linear counterparts. The micelle sizes of the graft copolymers could be tuned with chemical composition as well as temperature. Furthermore, when hydrophobic indomethacin was loaded into the micelles, the graft copolymer micelles trapped more indomethacin than PEO–PLA–PEO micelles, facilitating the in vitro control of the initial burst release of the drug. Drug release could be controlled in vitro by simply altering the EO/LA ratio of the grafting chains. The graft copolymer showed low cytotoxicity to 293T cells, indicating its great potential application for drug delivery.
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Nano Energy
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2015
·
IF 10.325
Pin Hao,Zhenhuan Zhao,Yanhua Leng,Jian Tian,Yuanhua Sang,Robert I. Boughton,C.P. Wong,Hong Liu,Bin Yang
Graphene-based nitrogen self-doped hierarchical porous carbon aerogels were synthesized for supercapacitor electrode application by using chitosan as a raw material through a carefully controlled aerogel formation–carbonization–activation process. The as-synthesized N-doped graphene-based carbon aerogels contained both macropores and mesopores from the aerogel preparation and carbonization process, and micropores from the chemical activation, confirmed by TEM, SEM, BET, etc. Because chitosan is a nitrogen-containing renewable biopolymer , the carbon aerogel derived from chitosan in this work was N-self-doped. The carbonized carbon aerogel was composed of a graphene framework and amorphous carbon , and the ratio between these two components was controlled by the activation temperature. With an increase in activation temperature, the amorphous carbon was etched away gradually, and a stable graphene portion remained to form a framework. Accordingly, the performance of the graphene-based carbon aerogel as a supercapacitor varied with increasing activation temperature. Electrochemical investigation measurements showed that the N-doped graphene-based hierarchical porous carbon aerogel represents a good electrode candidate for construction of a solid symmetric supercapacitor, which displays a high specific capacitance of about 197 F g −1 at a current density of 0.2 A g −1 . In addition, the solid state supercapacitor displayed excellent cyclability with a capacitance retention of about 92.1% over 10,000 cycles. The excellent energy storage ability of the chitosan-derived hierarchical graphene-based carbon aerogels is ascribed to the high conductivity of the graphene framework with nitrogen doping and the high storage ability of amorphous carbon with variable pore size and distribution.
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NANO
·
2014
·
IF 1.26
HUI LI,CHUANXI WANG,YINGNAN JIANG,ZHANCHEN CUI,QUAN LIN
Lanthanide-doped luminescent nanoscale materials have great potential applications in biological researches. Herein, we reported a novel and mild method for one-step synthesis of chitosan/ NaGdF 4 : Eu 3+ nanocomposites. The luminescent Eu 3+ ions and magnetic resonance imaging (MRI) contrast agent Gd 3+ ions were incorporated to these biocompatible nanocomposites. The resultant nanocomposites exhibited strong fluorescence and attractive magnetic features. The nanocomposites also have pure hexagonal phase with uniform size of about 65 nm. FT-IR spectra revealed that these nanocomposites were successfully coated by hydrophilic chitosan, whose amine groups conferred the nanocomposites excellent dispensability in aqueous solution. Besides, the MTT assay and laser confocal microscopy images have confirmed the good biocompatibility of the nanocomposites. These results indicated that the as-prepared nanocomposites could be used as an excellent targeted imaging agent in biological fields.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2014
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IF 3.107
Minhao Xie,Bing Hu,Yan Wang,Xiaoxiong Zeng
A new, simple, and effective method to graft gallic acid (GA) onto chitosan (CS) in aqueous solution in the presence of carbodiimide and hydroxybenzotriazole was developed. The grafting amount of GA reached as much as 209.9 mg/g of copolymer, which appears as the highest one among the reported literature, and the grafting degree of GA to CS was adjustable with modulation of the mass ratio of GA to CS. The covalent insertion of GA onto the polymeric backbones was confirmed by UV–vis and 1H NMR analyses. Grafting endowed the resulting copolymer GA-grafted-CS (GA-g-CS) with both the advantages of CS and GA. The antioxidant capacity of GA-g-CS was much higher than that of the plain CS examined by assays of DPPH, superoxide, and ABTS radicals scavenging activities, reducing power, chelating power, inhibition of lipid peroxidation, ferric reducing antioxidant potential, and β-carotene-linoleic acid assays. Particularly, GA-g-CS showed significantly higher antioxidant activity than GA in β-carotene-linoleic acid assay. Furthermore, the viscosity of GA-g-CS was significantly higher than that of CS. The present study developed a novel approach to synthesize GA-g-CS that could be a potential biomaterial in food industries.
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JOURNAL OF MOLECULAR LIQUIDS
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2014
·
IF 2.083
Lei Zhang,Zhengjun Cheng,Xiao Guo,Xiaohui Jiang,Rong Liu
We report an investigation of the title process. The corresponding process variables such as the concentration of surfactant, temperature, initial concentration of dye have been optimized by the response surface methodology (RSM). A 2 3 full factorial design was employed to analyze the individual and interaction effects of the process variables. The experimental data have been well-fitted to second order polynomial equations and the models were also examined using the analysis of variance and t test statistics. The obtained optimal conditions are as following: C s = 30.92 μM, T = 20 °C and C OG = 320 mg/L for the orange G , and C s = 34.10 μM, T = 50 °C and C OA = 500 mg/L for the acid orange 7. The measured experimental maximum adsorption capacities for the orange G and acid orange 7 systems under above optimization conditions were 1452.07 and 2352.99 mg/g, which were in good agreement with their corresponding predicted values (1427.13 and 2357.31 mg/g), with small relative errors of 1.72% and − 0.18%, respectively. Moreover, many aspects of the adsorption kinetics and isotherms for the two dyes are presented. The results indicated that the two adsorption equilibriums were achieved within 210 and 240 min for the acid orange 7 and orange G, the pseudo-second-order model was followed, and Langmuir isotherm model could be well described their adsorption data. In addition, the adsorption mechanism of two dyes onto chitosan/surfactant was discussed further by FTIR and X-ray diffraction (XRD) methods.
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