SEPARATION AND PURIFICATION TECHNOLOGY
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2026
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IF 9.1
Bin Wen,Fan Chen,Yi Ran,Dongyun Que,Zhihao Zhang,Youwei Zeng,Yuyuan Yao
Molybdenum disulfide (MoS₂) is a promising inorganic co-catalyst for Fenton processes due to its structural durability and avoidance of secondary organic pollution. However, the conventional 2H-phase MoS₂ suffers from intrinsically low conductivity, severely limiting Fe 3+ reduction in the Fenton reaction. Herein, L-cysteine served as a multifunctional precursor during the preparation of MoS₂, promoting a slow-release sulfur supply, carbon‑nitrogen co-doping, and the prevention of restacking. The carbon and nitrogen co-doped molybdenum disulfide (CN-MoS 2 ) architecture was fabricated via a single-step hydrothermal reaction mediated by sodium molybdate, thiourea, and L-cysteine. Remarkably, benefiting from the introduction of L-cysteine, the resulting CN-MoS 2 exhibited a high 1 T-phase content (68%), abundant sulfur vacancies, and a substantially reduced particle size. CN-MoS 2 accomplished near-complete elimination of carbamazepine (CBZ) within merely 3 min, yielding a prominent apparent rate constant of 0.7111 min −1 , which is a 19-fold kinetic acceleration over thiourea-derived MoS 2 . CN-MoS₂ also exhibited high TOC removal, broad-spectrum degradation, and robust CBZ removal under various water conditions. Additionally, electrochemical analyses further verified that CN-MoS₂ reduced charge-transfer resistance and enriched electron density, thereby facilitating rapid electron shuttling to Fe 3+ . Concurrently, quenching experiments and electron paramagnetic resonance (EPR) diagnostics unambiguously validated SO 4 •- , 1 O 2 and O 2 •- as the driving reactive forces. Moreover, a continuous-flow device incorporating a CN-MoS 2 -based membrane exhibited outstanding performance in wastewater purification. This work offers a new strategy for designing high-activity co-catalysts for advanced oxidation processes.
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高影响因子
Stem Cell Reports
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2026
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IF 5.6
Zhe Yang,Xin Li,Niujian Wu,Ling Wang,Zhanjin Lu,Lingling Wang,Shisong Han,Hongyun Lu
Bone marrow mesenchymal stem cell (BMSC) lineage commitment contributes to metabolic bone diseases, but whether glucagon-like peptide-1 (GLP-1) regulates osteo-adipogenic fate remains unclear. Here, RNA sequencing was performed to identify potential pathways regulated by GLP-1 in BMSCs, followed by gain- and loss-of-function experiments. Furthermore, BMSC-specific Hif-2α knockout mice with glucocorticoid-induced osteoporosis were treated with semaglutide to evaluate skeletal effects. GLP-1 suppressed adipogenesis and promoted osteogenesis of BMSCs in a dose-dependent manner. Transcriptomic analysis identified PI3K-AKT and hypoxia signaling as major GLP-1-regulated pathways. Mechanistically, GLP-1 inhibited AKT activation during adipogenesis and reshaped AKT signaling dynamics during osteogenesis. Loss of HIF-2α largely abolished GLP-1-mediated regulation of AKT activity. In vivo , semaglutide improved trabecular bone mass in osteoporotic mice, whereas this bone-protective effect was markedly diminished in Hif-2α -knockout mice. Our findings suggest that GLP-1 modulates BMSC lineage commitment by inhibiting adipogenesis and enhancing osteogenesis through HIF-2α-associated regulation of AKT signaling.
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PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
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2019
·Vol. 166
·期 16
·D908
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IF 3.12
Xinkuai He,Zhengyang Sun,Qingtian Zou,Jingjing Yang,Luye Wu
The codeposition behavior of Co(II) and Ni(II) in 1-ethyl-3-methylimidazolium bisulfate ([EMIM]HSO4) ionic liquid (IL) and ethylene glycol (EG) system is recorded using cyclic voltammetry (CV). The result indicates that the mechanism of Co-Ni codeposition is an anomalous codeposition, and both the inhibition for Ni(II) reduction and the anomalous codeposition phenomena can be improved by the increase of Co(II) concentration in the system. These Co-Ni deposits are characterized using EDS, XRD and SEM. SEM micrographs indicate that the surface morphology of Co-Ni deposits can be changed by the increase of Co content, and these deposits with many additional elongated acerose crystals, which are germinated on the spherical “nodules” surface, can be prepared. XRD pattern reveals the feature peak of crystalline Co1.2Ni2.8 with a preferred orientation direction and the average size of grains is calculated to be about 14.6 nm. Moreover, the electrocatalytic activity for hydrogen evolution reaction (HER) on the prepared Co-Ni catalyst surface is investigated using linear sweep voltammetry (LSV) and electrochemical impedance spectra (EIS). These results indicate that the obtained catalyst presents an excellent electrocatalytic activity and durability for HER with an overpotential of 139 mV at 10 mA cm−2 in 1 M KOH solution.
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Frontiers in Nutrition
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2026
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IF 5.5
Maoru Li,Zefeng Wang,Huan Zhai,Jiajiao Li,Ji Ma,Bin Qiu
BackgroundMetabolic dysfunction-associated steatotic liver disease (MASLD) is a rapidly growing metabolic disorder with no FDA-approved treatment. Pachymic acid (PA; synonym: 3-O-acetyltumulosic acid), a lanostane-type triterpenoid, shows potential for treating MASLD. However, its mechanisms remain underexplored.ObjectiveThis work evaluated the therapeutic efficacy and mechanisms of PA in treating MASLD.MethodsThe high-cholesterol diet (HCD)-induced zebrafish model, high-fat diet induced C57BL/6 mice model and free fatty acid (FFA)-treated BRL-3A cells was used to assess PA’s anti- MASLD effects in vivo and in vitro.ResultsPA improved lipid metabolism disrupted by HCD, reduced hepatic oxidative stress, as indicated by decreased MDA levels and increased SOD activity, and lowered inflammatory markers, including TNF-α, IL-6, and IL-1β. PA also enhanced antioxidant activity by regulating the Keap1-Nrf2-HO-1 pathway. PA decreased Keap1 mRNA expression induced by reactive oxygen species (ROS), upregulated Nrf2 and HO-1 expression, and promoted Nrf2 nuclear translocation. These effects suggest that PA inhibits ROS production by activating the Keap1-Nrf2-HO-1 pathway and possibly by inhibiting Nrf2 degradation.ConclusionPA improves MASLD through lipid metabolism regulation and antioxidant effects, primarily by activating the Keap1-Nrf2-HO-1 pathway. These findings highlight PA’s potential as a therapeutic agent for MASLD.
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高影响因子
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
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2026
·Vol. 1870
·期 11
·130988
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IF 2.9
Jingxian Han,Lei Hua,Siqi Zhang,Bin Yang,Zhiying Li,Xiangguang Meng
Background Heart failure (HF) remains a leading cause of death worldwide. Stem cells represent a promising strategy for myocardial regeneration due to their differentiation potential and paracrine effects. This study investigated the effect of human pericardial fluid Sca-1 + /Nanog + cells Conditioned Medium (hPFCs-CM) on doxorubicin (DOX)-induced damage to AC16 cardiomyocytes using transcriptomic analysis. Methods Human AC16 cardiomyocytes were divided into Control (CON), DOX, DOXh, and hPFCs groups. Cell viability, cytotoxicity (LDH release), reactive oxygen species (ROS) levels, and apoptosis were assessed. Transcriptomic sequencing was performed on CON, DOX, and DOXh groups. MYH3 and IFI16 genes were knocked down using siRNA, followed by Western blot analysis of apoptosis. Results DOX significantly increased ROS and apoptosis compared with CON group ( P < 0.05), while hPFCs-CM attenuated these effects ( P < 0.05). RNA-seq identified 52 differentially expressed genes between DOX and DOXh groups (adj P < 0.05). Three target genes ( MYH3 , IFI16 , and KCTD14 ) were selected for RT-qPCR validation. Further functional assays revealed that MYH3 knockdown significantly potentiated the anti-apoptotic activity of hPFCs-CM ( P < 0.05), whereas IFI16 knockdown showed no significant effect. Conclusion hPFCs-CM effectively attenuated DOX-induced AC16 cardiomyocyte damage through MYH3, providing potential molecular targets for future mechanistic studies in myocardial injury treatment.
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高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
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2026
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IF 3.5
Linlin Zhang,Yanfen Chen,Fangyu Wu,Aoxing Feng,Jiaqi Zhang,Tianyu Zhang,Xuenan Yang,Sisi Li,Qilin Lu,Jingjing Hou,Yuanhui Tang,Lixin Yu,Xiaolin Wang,Yakai Lin
Plasma leakage and thrombosis remain critical challenges limiting the long-term performance of poly(4-methyl-1-pentene) (PMP) hollow fiber membranes (HFMs) in extracorporeal membrane oxygenation (ECMO). In this work, a hierarchical hydrophobic modification strategy was developed to construct a stable anti-wetting interface on PMP HFMs. Surface hydroxyl groups were first generated via Fenton-induced oxidation, followed by interfacial coupling with trimesoyl chloride (TMC). Subsequently, SiO 2 nanoparticles were grafted to create a micro/nano-structured rough surface, and further modified with 1H,1H,2H,2H-perfluorodecyltrimethoxysilane (17-FAS) to reduce surface energy. Under the optimized conditions (120 min Fenton pre-treatment, 60 min TMC reaction, and 0.1% (mass) SiO 2 nanoparticles), the modified PMP–SiO 2 /17-FAS membrane exhibited a high N 2 flux of 54.1 ml·cm –2 ·min –1 ·MPa –1 , while the plasma leakage resistance time was significantly prolonged from 5910 to 8775 min. In addition, bovine serum albumin adsorption was reduced by approximately 50%, indicating enhanced antifouling performance. Coagulation tests further revealed a selective prolongation of activated partial thromboplastin time, while prothrombin time, thrombin time, and international normalized ratio remained nearly unchanged, suggesting suppressed contact-induced coagulation activation without disturbing normal coagulation pathways. The enhanced performance could be attributed to the synergistic effect of micro/nano-structured roughness and the fluorinated low-surface-energy layer, which stabilizes a non-wetting interfacial state and reduces protein-surface interactions. This work provides a feasible strategy for simultaneously improving plasma leakage resistance and hemocompatibility of PMP-based oxygenation membranes. Download: Download high-res image (297KB) Download: Download full-size image
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ADVANCED MATERIALS
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2026
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IF 29.1
Shuo Zhang,Yuyang Lu,Chong Yan,Xiangbiao Liao,Chen-Zi Zhao,Jun-Wei Zhao,Zhiyuan Dong,Zhenwei Zhu,Wenjie Meng,Xue-Fei Wen,Peng Wu,Jian Pei,Meng-Yao Wang,Xue-Kun Cao,Jiang-Kui Hu,Xiang Chen,Jingyi Qiu,Hao Zhang,Jia-Qi Huang,Qiang Zhang
The pursuit of high-energy-density lithium metal batteries requires simultaneous optimization of electrode architecture, electrolyte formulation, and interfacial stability. Here, we establish a fundamental parameter g(σ e , D e ) that quantifies the relationship between electrolyte dosage and capacity utilization in ultra-thick electrodes (>100.0 µm), enabling precise determination of the minimal electrolyte requirement (1.1 g Ah −1 ). Through systematic investigation of electrolyte compatibility with high-loading cathodes (> 10.0 mAh cm −2 ) at high voltages (4.8 V), we develop an optimized formulation that forms stable interfaces while suppressing parasitic reactions. By integrating these advances—including a lightweight lithium metal anode—we demonstrate a 54.2 Ah pouch cell achieving 769 Wh kg −1 , representing a 150% improvement over conventional lithium-ion batteries. This work provides both theoretical and practical frameworks for engineering next-generation batteries through electrolyte minimization and interface stabilization.
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高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2026
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IF 5.9
Runfeng Jia,Qingliang Chen,Khan Md Sabur,Zhongyu He,Bingxue Chen,Kaidi Hu,Xiaoqing Zhao,Hanbing Rao,Zhiwei Lu,Yanying Wang,Xing Wei,Mengmeng Sun
A single oral Lut-NPs/Gel system synergistically ameliorates both colitis and associated depression-like behaviors via the gut-brain axis. • Lut-NPs/Gel combats colitis via a synergistic combination of barrier repair, anti-inflammatory action, and beneficial microbiome remodeling. • Restoration of AKK abundance plays a critical regulatory role in gut microbiota remodeling. • Multi-omics analyses identified key modulated pathways, including TRP channels and neuroactive ligand-receptor interactions, functionally connecting gut repair to brain behavioral improvement. • Recovery of AKK in normal state is coordinated with upregulation of host repair genes (such as IGF1, Reg1), delineating a collaborative host-microbe network. Inflammatory bowel disease (IBD) is a chronic immune-mediated condition affecting the intestinal tract. Though conventional therapies aim to control symptoms and induce remission, their long-term use is hindered by systemic side effects and inadequate efficacy, highlighting the need for safer and more effective treatments. In this study, we developed luteolin-loaded nanoparticles (Lut-NPs) by encapsulating luteolin within metal-phenolic networks (MPNs) formed through coordination between epigallocatechin gallate (EGCG) and Ce 3 ⁺ ions. The resulting Lut-NPs showed excellent biocompatibility, strong antioxidant and anti-inflammatory activities, and remarkable stability under gastrointestinal conditions. To improve intestinal retention, the nanoparticles were incorporated into an inulin-based hydrogel, generating a Lut-NPs/Gel composite system. In a DSS-induced colitis model, oral administration of Lut-NPs/Gel demonstrated significant therapeutic efficacy, including restoration of the intestinal barrier, suppression of pro-inflammatory pathways, and reshaping the gut microbiome. The treatment also promoted macrophage polarization from the M1 to the M2 phenotype. AKK, usually considered beneficial but elevated during inflammation, was restored to normal levels after Lut-Nps/Gel treatment, suggesting its potential as a biomarker of intestinal recovery. Additionally, the treatment alleviated depression-like behaviors via the microbiota–gut–brain axis. Overall, this work provides a safe and promising multifunctional strategy for treating IBD and associated neuropsychiatric symptoms.
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高影响因子
ANNALS OF MICROBIOLOGY
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2026
·Vol. 76
·期 1
·12
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IF 3.4
Li Haodong,Tao Xian,Zhang Hongji,Zhang Yafei,Liang Qiancheng,Liu Qing,Luo Lan,Wang Yan,Yang Juan,Yang Xiaosheng,Peng Mei,Li Liangqun
Purpose The aim of this study was to isolate and identify a nicotine-degrading Paenarthrobacter nicotinovorans strain from the Epimedium rhizosphere , characterize its secondary metabolites, and evaluate its potential antioxidant activity against premature ovarian failure (POF). By exploring the bioactive properties of this rhizosphere microorganism, the research sought to uncover new insights into the functional roles of plant-associated microbes and discover potential natural agents for treating oxidative stress-related diseases. Methods A strain designated YYH-1 was isolated from the E. rhizosphere and identified through morphological observations and 16S rRNA gene sequencing. Ultra-performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS) combined with GNPS molecular networking was employed to profile the chemical components of the ethyl acetate extract from YYH-1 fermentation broth. In vitro experiments utilized the human ovarian granulosa cell line (KGN) to assess the extract’s effect on D-galactose-induced cellular senescence, measuring the activity of superoxide dismutase (SOD) as an indicator of antioxidant capacity. Additionally, molecular docking was performed to elucidate the interaction mechanisms between key compounds and the Nrf2 signaling pathway. Results The isolate was confirmed as P. nicotinovorans YYH-1. Chemical profiling identified 21 secondary metabolites, including flavonoids, phenylpropanoids, alkaloids, fatty acids, and amino acid derivatives. In vitro studies demonstrated that the YYH-1 extract significantly mitigated D-galactose-induced cellular senescence in KGN cells and effectively upregulated superoxide dismutase (SOD) activity, which increased by 50.94%, 48.27%, and 38.01% respectively compared with that in the Model group.. Molecular docking analysis revealed that 8-prenylnaringenin, a major compound in the extract, could activate the Nrf2 signaling pathway by stably binding to Keap1 and Nrf2 receptors. Conclusion This study represents the first report of P. nicotinovorans in the E. rhizosphere . The strain exhibits the potential to produce diverse bioactive secondary metabolites, and its extract demonstrates antioxidant activity against POF through the activation of the Nrf2 pathway. These findings expand our understanding of rhizosphere microbial functions and provide a basis for the development of natural anti-aging therapies targeting oxidative stress-related disorders.
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高影响因子
PROCESS BIOCHEMISTRY
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2026
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IF 4.4
Liyang Xu,Yujia Wang,Feiyu Yan,Wenjing Pan,Yingjia Tong,Jinsong Gong,Xiaomei Zhang,Jinsong Shi,Zhenghong Xu,Hui Li
Mycolicibacterium is a crucial industrial host for steroid biotransformation, yet its genetic engineering has long been limited by inefficient homologous recombination (HR) and strong non-homologous end joining (NHEJ) activity. Here, we established and optimized a suicide plasmid-based genome editing system for Mycobacterium sp. LY-1. The constructed vector p2NILB, combining kanamycin selection with sacB-mediated counter-selection, enabled precise and markerless allelic exchange. Suppression of NHEJ through deletion of ku or ligD , together with optimization of homology arm length, increased gene knockout efficiency to over 70%. This optimized platform was subsequently applied to metabolic engineering of sterol degradation to enhance 9α-hydroxyandrostenedione (9α-OH-AD) biosynthesis. Sequential deletion of five kstD isoenzymes blocked downstream steroid degradation, with kstD1 identified as the major contributor to product loss. Functional analysis revealed KshA2 as the key 9α-hydroxylase, and its overexpression increased the 9α-OH-AD titer to 3.41 g/L. In addition, deletion of the branched-chain acid transporter gene mmpL3 further enhanced 9α-OH-AD production to 4.19 g/L. To overcome substrate utilization limitations under high phytosterol loading conditions, a soybean oil‑Tween 80 solvent system overcame substrate limitation, enabling 15.71 g/L 9α‑OH‑AD from 30 g/L phytosterols. This study provides a robust genome editing framework and an effective strategy for engineering steroid-producing actinobacteria.
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高影响因子
AQUACULTURE NUTRITION
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2026
·Vol. 2026
·期 1
·4395252
·
IF 4.6
Haoran Yin,Lixing Wang,Zexin Lin,Ruilin Li,Qinxiao Cai,Zhiyu Zhou,Xiaoyi Wu
The hybrid grouper ( Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂), a typical carnivorous fish, has a limited ability to utilize dietary carbohydrates (CHOs). This study investigated the effects of different dietary arginine (Arg) levels (2.55 and 3.65%) and CHO-to-lipid ratios (C/L: 3.57, 2.39, 1.61) on the glycometabolism of hybrid grouper. Juvenile hybrid grouper (mean initial body length and weight: 7.8 cm and 13.04 g) were manually fed to fullness two times per day throughout a 7-week growth trial. The results showed that the C/L3.67 diets significantly elevated 2-h postprandial blood glucose concentration, as well as reduced hepatic glycogen level, intraperitoneal fat ratio (IPF), and the whole-body and hepatic lipid contents relative to the C/L2.39 and C/L1.61 diets. Dietary Arg supplementation significantly improved this metabolic disorder. These improvements were associated with enhanced plasma insulin-like growth factor 1 (IGF-1) concentration, hepatic phosphofructokinase (PFK) activity, nitric oxide (NO), and cyclic guanosine monophosphate (cGMP) levels. They were also linked to the upregulated expression of key molecules in related pathways, including IGF-1 receptor (igf1r), phosphatidylinositol 3-kinase (pi3k), protein kinase AMP-activated catalytic subunit α (ampk-α), and protein kinase B (Akt) genes, as well as glucose transporter 4 (GLUT4) and mechanistic target of rapamycin (mTOR) proteins. Generally, suitable dietary Arg supplementation can reduce postprandial blood glucose and hepatic glycogen concentrations in hybrid grouper fed high C/L diets by regulating key genes and proteins within the IGF-1 and NO-cGMP pathways. This finding provided a nutritional intervention to enhance CHO utilization in hybrid grouper aquaculture.
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高影响因子
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
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2026
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IF 5.3
Zhu Guo,Yujing Zhang,Ping Zou,Caiyun Sun,Liya Cui,Gang Wang,Chao Wang
A novel class of 6-aryl-1-(3,4,5-trimethoxyphenyl)-1H-pyrazolo[3,4-b]pyrazine derivatives was designed and synthesized as tubulin polymerization disruptors. Among them, compound 10t emerged as the most potent agent, exhibiting IC₅。 values of 0.13-0.18 µM against HeLa, SGC‑7901, and MCF‑7 cancer cell lines. Functional studies revealed that 10t effectively inhibits tubulin assembly in vitro, destabilizes cellular microtubule networks, induces G₂/M cell‑cycle arrest, and triggers apoptosis. Molecular docking indicated that 10t binds to the colchicine site on tubulin, forming a unique hydrogen bond with β‑Asn349 that is not observed with the reference compound CA‑4. Importantly, 10t showed significantly lower cytotoxicity toward normal HUVECs than CA‑4, suggesting an improved safety profile. Collectively, these findings establish the pyrazolo[3,4‑b]pyrazine scaffold as a promising platform for microtubule‑targeting anticancer agents and identify 10t as a compelling lead for further development.
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