Nanoscale Research Letters
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2018
·Vol. 13
·期 1
·1-7
·
IF 3.125
Wang Jie,Li Meisheng,Qian Ming,Zhou Shouyong,Xue Ailian,Zhang Lili,Zhao Yijiang,Xing Weihong
Abstract TMC-incorporated carbon nitride (CN) with hexagonal and quadrangle honeycomb-like structure and having periodic lattice defects linked by –CONH– bond was synthesized through combining the high calcination with the chemical condensation of melamine and 1,3,5-benzenetricarbonyl trichloride. The obtained CN has a tri-s-triazine ring and benzene ring skeleton, which makes it have excellent mechanical and thermal stability. The BET specific surface area was enhanced to about 125.6 m 2 /g, and the mean pore size is about 3.43 nm. This CN exhibited an excellent adsorption-enhanced photocatalytic performance. Graphical abstract
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JOURNAL OF NANOBIOTECHNOLOGY
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2024
·Vol. 22
·期 1
·1-20
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IF 10.6
Liu Ying,Tang Rui,Cao Yuting,Wu Nianhong,Qin Qiaoxi,Chen Yuanyuan,Wei Xi,Ren Jianli,Sun Yang,Zhou Hong,Zhou Yang,Li Pan
Background Vasculogenic mimicry (VM), when microvascular channels are formed by cancer cells independent of endothelial cells, often occurs in deep hypoxic areas of tumors and contributes to the aggressiveness and metastasis of triple-negative breast cancer (TNBC) cells. However, well-developed VM inhibitors exhibit inadequate efficacy due to their low drug utilization rate and limited deep penetration. Thus, a cost-effective VM inhibition strategy needs to be designed for TNBC treatment. Results Herein, we designed a low-intensity focused ultrasound (LIFU) and matrix metalloproteinase-2 (MMP-2) dual-responsive nanoplatform termed PFP@PDM-PEG for the cost-effective and efficient utilization of the drug disulfiram (DSF) as a VM inhibitor. The PFP@PDM-PEG nanodroplets effectively penetrated tumors and exhibited substantial accumulation facilitated by PEG deshielding in a LIFU-mediated and MMP-2-sensitive manner. Furthermore, upon exposure to LIFU irradiation, DSF was released controllably under ultrasound imaging guidance. This secure and controllable dual-response DSF delivery platform reduced VM formation by inhibiting COL1/pro-MMP-2 activity, thereby significantly inhibiting tumor progression and metastasis. Conclusions Considering the safety of the raw materials, controlled treatment process, and reliable repurposing of DSF, this dual-responsive nanoplatform represents a novel and effective VM-based therapeutic strategy for TNBC in clinical settings. Graphical Abstract
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ACTA PHARMACOLOGICA SINICA
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2024
·
IF 6.9
Han Yu,Zhang Jia-qi,Ji Ya-wei,Luan Yi-wen,Li Shu-yi,Geng Hui-zhen,Ji Ying,Yin Cui,Liu Su,Zhou Chun-yi,Xiao Cheng
Nicotinic acetylcholine receptors (nAChRs) regulate pain pathways with various outcomes depending on receptor subtypes, neuron types, and locations. But it remains unknown whether α4β2 nAChRs abundantly expressed in the substantia nigra pars reticulata (SNr) have potential to mitigate hyperalgesia in pain states. We observed that injection of nAChR antagonists into the SNr reduced pain thresholds in naïve mice, whereas injection of nAChR agonists into the SNr relieved hyperalgesia in mice, subjected to capsaicin injection into the lower hind leg, spinal nerve injury, chronic constriction injury, or chronic nicotine exposure. The analgesic effects of nAChR agonists were mimicked by optogenetic stimulation of cholinergic inputs from the pedunculopontine nucleus (PPN) to the SNr, but attenuated upon downregulation of α4 nAChRs on SNr GABAergic neurons and injection of dihydro-β-erythroidine into the SNr. Chronic nicotine-induced hyperalgesia depended on α4 nAChRs in SNr GABAergic neurons and was associated with the reduction of ACh release in the SNr. Either activation of α4 nAChRs in the SNr or optogenetic stimulation of the PPN-SNr cholinergic projection mitigated chronic nicotine-induced hyperalgesia. Interestingly, mechanical stimulation-induced ACh release was significantly attenuated in mice subjected to either capsaicin injection into the lower hind leg or SNI. These results suggest that α4 nAChRs on GABAergic neurons mediate a cholinergic analgesic circuit in the SNr, and these receptors may be effective therapeutic targets to relieve hyperalgesia in acute and chronic pain, and chronic nicotine exposure.
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Frontiers in Energy
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2025
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IF 6.2
Zhao Zihao,Feng Xurui,Feng Daili,Li Chengming,Feng Yanhui,Wei Junjun
The use of porous skeletons for encapsulating phase change materials (PCMs) is an effective approach to addressing issues such as leakage, low thermal conductivity, and poor photothermal conversion efficiency. Inspired by the hollow skeletal structure found in birds in nature, high-quality 3D interconnected hollow diamond foam (HDF) was fabricated using a series of processes, including microwave plasma chemical vapor deposition (CVD), laser perforation, and acid immersion. This HDF was then used as a scaffold to encapsulate PEG2000. The results demonstrate that HDF significantly reduces the supercooling degree and latent heat discrepancy of PEG2000. Compared to pure PEG2000, the thermal conductivity of the HDF/PEG increased by 378%, while its latent heat reached 111.48 J/g, accompanied by a photothermal conversion efficiency of up to 86.68%. The significant performance improvement is mainly attributed to the combination of the excellent properties of the diamond with the inherent advantages of the 3D interconnected structure in HDF, which creates a high-conductivity transport network inside. Moreover, the HDF/PEG composite extends the temperature cycling time of electronic components by 4 times for heating and 2.3 times for cooling, thereby prolonging the operational lifetime of electronic devices. HDF/PEG offers an integrated solution for solar energy collection, photothermal conversion, heat dissipation in electronic components, and thermal energy transfer/storage. This innovative approach provides innovative ideas for the design and fabrication of composite PCMs and has great application potential, such as solar energy utilization, thermal management, and thermal energy storage.
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JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
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2025
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IF 3.2
Zhu Mengxuan,Liu Yifan,Ma Jiaxin,Wang Longyang,Wang Rui,Jiang Heng,Jin Changzi
Developing the novel green routes for synthesis of important aluminosilicate zeolites is of significant for theoretical research and practical application. In this work, MCM-22 zeolite with MWW-topology was synthesized through seed-assisted interzeolite transformation from FAU zeolite without using additional organic template. The key influence factor to the synthesis process was investigated and the zeolite products were characterized by a series of modern techniques. It has been shown that not only the presence of MCM-22 seed, but also the appropriate SiO 2 /Al 2 O 3 and Na 2 O/SiO 2 molar ratio of starting synthesis gel with were crucial to synthesize pure MWW zeolite. The hexamethyleneimine template occluded in seed also played a key role in successful interzeolite transformation, but did not participate structure-directing of MWW product. Although the zeolite synthesized by interzeolite transformation showed lower surface area and pore volume compared with MCM-22 zeolite prepared by conventional route, the Pt modified sample exhibited promising catalytic performance in hydroisomerization of n -heptane. Graphical
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Nature Communications
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2025
·Vol. 16
·期 1
·11294
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IF 15.7
Chen Zhezhe,Zhu Qiongjun,Xu Hong,Hu Yiqing,Wang Yanan,Chen Zhebin,Wang Yao,Huang Xingru,Fu Guosheng,Ma Boxuan,Zhang Wenbin
Many patients are suffering from atherosclerosis without typical risk factors, which can cause severe cardiovascular complications. Trimethylamine N-oxide (TMAO), derived from gut microbes, is a key unconventional contributor to the development of atherosclerosis. Here we present a strategy performed by orally administered nano-functionalized probiotics (PDMF@LGG) to inhibit TMAO through the gut microbiota-trimethylamine (TMA)-TMAO axis. PDMF@LGG, composed of polydopamine-coated Lacticaseibacillus rhamnosus GG and nanoparticles based on a reactive oxygen species (ROS)-responsive polymeric prodrug of fluoromethylcholine (FMC), can promote the retention of probiotics and nanoparticles in the intestine to persistently scavenge elevated ROS and release drugs. This process suppresses TMA production and absorption, lowering plasma TMAO levels. The therapeutic effects on male ApoE −/− mice demonstrate that PDMF@LGG reduces TMAO levels, alleviates atherosclerotic plaque formation, and regulates gut microbial composition and various metabolites. Together, PDMF@LGG represents a potential candidate for atherosclerotic therapy caused by TMAO and broadens the range of treatable atherosclerosis.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2016
·Vol. 408
·期 26
·7491-7503
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IF 3.125
Yang Ting,Xu Feifei,Sheng Yuan,Zhang Wen,Chen Yun
Apoptosis suppression caused by overexpression of anti-apoptotic proteins is a central factor to the acquisition of multi-drug resistance (MDR) in breast cancer. As a highly conserved anti-apoptotic protein, Bcl-2 can initiate an anti-apoptosis response via an ERK1/2-mediated pathway. However, the details therein are still far from completely understood and a quantitative description of the associated proteins in the biological context may provide more insights into this process. Following our previous attempts in the quantitative analysis of MDR mechanisms, liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based targeted proteomics was continually employed here to describe ERK/Bcl-2-mediated anti-apoptosis. A targeted proteomics assay was developed and validated first for the simultaneous quantification of ERK1/2 and Bcl-2. In particular, ERK isoforms (i.e., ERK1 and ERK2) and their differential phosphorylated forms including isobaric ones were distinguished. Using this assay, differential protein levels and site-specific phosphorylation stoichiometry were observed in parental drug-sensitive MCF-7/WT cancer cells and drug-resistant MCF-7/ADR cancer cells and breast tissue samples from two groups of patients who were either suspected or diagnosed to have drug resistance. In addition, quantitative analysis of the time course of both ERK1/2 and Bcl-2 in doxorubicin (DOX)-treated MCF-7/WT cells confirmed these findings. Overall, we propose that targeted proteomics can be used generally to resolve more complex cellular events.
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Chinese Journal of Integrative Medicine
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2019
·Vol. 26
·期 2
·138-145
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IF 1.445
Deng Chu-xin,Wu Zhi-bing,Chen Yi,Yu Zheng-miao
Objective To investigate the neuromodulatory effect of pinellia total alkaloids (PTA) on the gamma-aminobutyric acidergic (GABAergic) system in epileptic rats, and preliminarily evaluate the anti-epileptic effect of PTA. Methods Ninety-one male Sprague-Dawley rats were randomized to a control group ( n =17) or an epileptic group ( n =74) using computer-generated random numbers. Status epilepticus (SE) was induced with pilocarpine in the epileptic group. Epileptic rats that survived SE were randomly divided into 4 groups, namely an epilepsy group ( n =13), a topiramate (TPM, 60 mg/kg) group ( n =12), a high-dose PTA (800 mg/kg) group ( n =12), and a low-dose PTA (400 mg/kg) group ( n =10). Treatments were given intragastrically once daily for 14 days. The control group and epilepsy group received normal saline. Spontaneous recurrent seizures (SRSs) were monitored 8-h daily for 7 days after treatment. Then, the hippocampal formation tissues were collected. GABA level was measured using enzyme-linked immunosorbent assay. Protein and mRNA expression levels of glutamate decarboxylase 65 (GAD65), GABA transporter-1 (GAT-1), GABA transaminase (GABA-T), and GABA A receptor (GABA A R) α4, α5, γ2 and δ subunits were measured using Western-blotting analysis and quantitative polymerase chain reaction. Results PTA lowered the incidence and frequency of SRS (both doses vs. the TPM group, P >0.05). Compared with the epilepsy group, PTA increased the levels of GABA (both doses P <0.01) and GAD65 (mRNA, 800 mg/kg, P <0.01), and suppressed the levels of GAT-1 (mRNA, 800 mg/kg, P <0.01; 400 mg/kg, P <0.05), GABA-T (mRNA, both doses P <0.01), and GABA A R δ subunit (protein, 800 mg/kg, P <0.05) and γ2 subunit (protein, both doses P <0.01). PTA upregulated the low-expressed mRNA levels of GABA A R α5 subunit (400 mg/kg, P <0.01), δ subunit (800 mg/kg, P <0.05), and γ2 subunit (400 mg/kg, P <0.05). Conclusions PTA regulated the GABAergic system through modulating GABA levels and the expression levels of GAD65, GAT-1, GABA-T, and GABA A R α4, α5, γ2 and δ subunits. PTA may exert anti-epileptic effects on the pilocarpine-induced epilepsy model.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2022
·Vol. 414
·期 12
·3697-3708
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IF 4.478
Wahid Amir,Sohail Amir,Wang Huiyu,Guo Miao,Zhang Lu,Ji Yin,Wang Peng,Xiao Hua
Extracellular vesicles (EVs) play critical roles in intercellular communications, which contain valuable biomarkers for the detection of cancers. Phosphoproteomics analysis of human saliva EVs (sEVs) can help to discover lung cancer–related candidates. Due to the low abundance of phosphoproteins in sEVs, an efficient, reproducible, and cost-effective strategy is required for their enrichment. Here, we compared the latest phosphopeptide techniques, including TiO 2 , ZrO 2 , CaTiO 3 , and Ti 4+ -IMAC (immobilized metal affinity chromatography) methods, for phosphopeptide isolation. Our data demonstrated that Ti 4+ -IMAC was the superior one. By using the optimized Ti 4+ -IMAC approach, we identified more than 500 sEV phosphopeptides. Quantitative proteomics was employed to comprehensively decipher the sEV phosphoproteome of the normal group ( n = 6) and lung cancer group ( n = 6). Accordingly, 524 and 333 phosphopeptides were enriched, respectively, which corresponded to 439 and 282 phosphoproteins. In total, 857 unique sEV phosphopeptides corresponding to 721 phosphoproteins were revealed. Among 493 identified phosphosites, 37 were upregulated (> 1.5) and 217 were downregulated (< 0.66) in the cancer group. Our data collectively demonstrated that Ti 4+ -IMAC is an efficient and reproducible technology for comprehensive analysis of sEV phosphoproteome. Differentially expressed sEV phosphoproteins and phosphosites might be used for the detection of lung cancer non-invasively. Graphical abstract
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Neurotherapeutics
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2021
·Vol. 18
·期 2
·1064-1080
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IF 7.62
Wang Ni-Ya,Li Jin-Nan,Liu Wei-Lin,Huang Qi,Li Wen-Xing,Tan Ya-Hong,Liu Fang,Song Zi-Hua,Wang Meng-Yue,Xie Ning,Mao Rong-Rong,Gan Ping,Ding Yu-Qiang,Zhang Zhi,Shan Bao-Ci,Chen Li-Dian,Zhou Qi-Xin,Xu Lin
Brain capillaries are crucial for cognitive functions by supplying oxygen and other nutrients to and removing metabolic wastes from the brain. Recent studies have demonstrated that constriction of brain capillaries is triggered by beta-amyloid (Aβ) oligomers via endothelin-1 (ET1)-mediated action on the ET1 receptor A (ETRA), potentially exacerbating Aβ plaque deposition, the primary pathophysiology of Alzheimer’s disease (AD). However, direct evidence is still lacking whether changes in brain capillaries are causally involved in the pathophysiology of AD. Using APP/PS1 mouse model of AD (AD mice) relative to age-matched negative littermates, we identified that reductions of density and diameter of hippocampal capillaries occurred from 4 to 7 months old while Aβ plaque deposition and spatial memory deficit developed at 7 months old. Notably, the injection of ET1 into the hippocampus induced early Aβ plaque deposition at 5 months old in AD mice. Conversely, treatment of ferulic acid against the ETRA to counteract the ET1-mediated vasoconstriction for 30 days prevented reductions of density and diameter of hippocampal capillaries as well as ameliorated Aβ plaque deposition and spatial memory deficit at 7 months old in AD mice. Thus, these data suggest that reductions of density and diameter of hippocampal capillaries are crucial for initiating Aβ plaque deposition and spatial memory deficit at the early stages, implicating the development of new therapies for halting or curing memory decline in AD.
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MOLECULAR AND CELLULAR BIOCHEMISTRY
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2018
·Vol. 447
·期 1
·137-149
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IF 2.561
Wu Weiche,Wang Sisi,Xu Ziye,Wang Xinxia,Feng Jie,Shan Tizhong,Wang Yizhen
Betaine, a neutral zwitterionic compound, could regulate intramuscular fat (IMF) deposition and meat quality. However, the efficacy is controversial. Moreover, the regulatory mechanism of betaine on lipid metabolism in skeletal muscle cells remains unclear. Therefore, in this study, we examined the effects and regulatory mechanism of betaine on lipid accumulation in adipogenic-differentiated C2C12 cells. We found that adipogenic-induced C2C12 cells treated with 10 mM betaine for 24 and 48 h had more lipid accumulation than the control group. Real-time PCR and Western blot results revealed that betaine treatment did not alter the expression of lipolysis and lipid oxidation-related genes, but dramatically increased the expression of peroxisome proliferator-activated receptor γ (PPARγ) and its target genes such as fatty acid binding protein 4 (aP2), fatty acid synthase (FAS) and lipoprteinlipase (LPL). Furthermore, betaine combined with PPARγ inhibitor GW9662 treatment showed that betaine elevated C2C12 lipid accumulation through upregulation of PPARγ. Mechanistically, we found that betaine promoted PPARγ expression and lipid accumulation through inhibition of extracellular regulated protein kinases1/2 (ERK1/2) signalling pathway. These results demonstrate that betaine acts through ERK1/2-PPARγ signalling pathway to regulate lipid metabolism in adipogenic-differentiated skeletal muscle cells, which could provide some useful information for controlling muscle lipid accumulation by manipulating ERK1/2 and PPARγ signalling pathway.
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INTERNATIONAL JOURNAL OF ADVANCED MANUFACTURING TECHNOLOGY
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2026
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IF 3.7
Cui Xiangxiang,Wei Tianlu,Chen Xingqiang,Fang Yinghui,Yang Hai,Zhu Xingfei
Thin‑walled hard‑brittle curved‑surface quartz crucibles are critical high‑temperature components for Czochralski monocrystalline silicon growth. Inner‑wall defects such as chipping pits and black spots cause crucible rejection, whereas conventional planar chemical‑mechanical polishing (CMP) fails for curved‑surface defect remediation. Herein, a rare‑earth‑based photocatalysis‑assisted eco‑friendly CMP slurry was developed. Taguchi L25 (5 5 ) orthogonal tests were performed to optimize slurry formulation and processing parameters using areal roughness Sa, signal‑to‑noise ratio and mean roughness as criteria. White‑light interferometry and X‑ray photoelectron spectroscopy (XPS) were employed to characterize surface topography and interfacial chemistry, and crystal‑pulling trials evaluated the service performance of repaired crucibles. The optimal slurry contains 10 vol.% H 2 O 2 , 8 wt.% CeO 2 , pH 9.5, 0.8 wt.% CMC‑Na, 3 wt.% EDTA‑5Na, 3 wt.% TiO 2 and 1 wt.% lanthanum carbonate modifier. Optimal parameters are: normal load 12 N, polishing duration 60 min, illumination intensity 4000 Lux, rotational speed 3000 r/min, slurry flow rate 80 mL/min. Applying this integrated process to 32‑inch spent crucibles eliminates typical inner‑wall defects and yields ultra‑smooth surfaces with Sa 0.724–0.844 nm and seamless substrate‑polished‑zone transition. Comparative pulling tests reveal no statistically significant differences in key crystal‑growth metrics between repaired and brand‑new crucibles. XPS demonstrates that Ce‑based abrasives govern interfacial reactions: photocatalytically generated hydroxyl radicals produce silicate softening layers and surface Si‑OH groups, which condense with abrasive‑bound Ce‑OH to form Ce‑O‑Si bonds removed via mechanical shearing. CMC‑Na suppresses scratches and mitigates heavy‑load damage. This slurry‑photocatalysis synergy enables high‑value‑added remanufacturing of spent quartz crucibles and benefits material‑loss reduction for the photovoltaic silicon chain.
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