EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY
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2017
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IF 1.975
Ting Zhang,Ying Chen,Yang Yang,Zhonghui Wang,Qi Pan,Sichong Xu,Zuyue Sun
The purpose of this study is to identify the potential of a two-dimensional preantral follicle culture as an in vitro model of predicting premature ovarian failure . The two-dimensional preantral follicles culture method was established by cultivating preantral follicles collected from ICR F1 hybrids (aged 12–14 days) for 12 days. The preantral follicles were incubated with 0.54 mg/ml cyclophosphamide , 0.5 mg/ml busulfan , 0.12 mg/ml cisplatin , 3.12 mg/ml 4-vinylcyclohexene diepoxide, 5 mg/ml D (+) galactose , and 0.5 mg/ml hydrocortisone for 24 h at culture days 2, 6 and 11. The diameter of follicles, the cumulus-oocyte complex number and the maturity of oocytes were recorded as the parameters to detect follicular maturation induced by the culture agents. The results indicated that, except for busulfan, D (+) galactose, and hydrocortisone, such test articles could significantly decrease follicular growth ( p < 0.05 or p < 0.01), and induce oocyte degeneration and follicle atresia when the follicles were treated at day 2. With the exception of hydrocortisone, such agents also gradually decreased follicular development ( p < 0.05 or p < 0.01) when the follicles were treated at day 6. All of the test articles but hydrocortisone can interfere with the ovulation , the cumulus-oocyte complex discharge and oocyte maturation of follicles when treated at days 2, 6 and 11. It is suggested that two-dimensional preantral follicle culture could be utilized as a potential in vitro system to mimic the POF model. It may also be employed in screening potential ovarian toxic agents, reducing laboratory animal use and promoting animal welfare .
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INTERNATIONAL IMMUNOPHARMACOLOGY
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2017
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IF 2.956
Xiaobao Gong,You Yang,Ligua Huang,Qingyan Zhang,Rong-Zhen Wan,Peng Zhang,Baoshun Zhang
To evaluate the hepatoprotective effects and potential mechanisms of paeonol (Pae) against acute liver failure (ALF) induced by lipopolysaccharide (LPS)/ d -galactosamine ( d -GalN) in mice, we examined anti-oxidative, anti-inflammatory and anti-apoptotic activities of Pae. We found that Pae pretreatment markedly reduced the activities of alanine transaminase and aspartate transaminase as well as the histopathological changes induced by LPS/ d -GalN. Catalase , glutathione and superoxide dismutase activities increased and reactive oxygen species activity decreased after Pae treatment compared with LPS/ d -GalN treatment. Pretreatment with Pae also significantly inhibited the expression levels of iNOS, nitric oxide (NO), COX-2 and prostaglandin E 2 (PGE 2 ). In addition, Pae administration prevented the phosphorylated expression of IκB kinase, inhibitor kappa B in the nuclear factor-kappa B (NF-κB) signaling pathway , and suppressed the phosphorylated expression of extracellular signal-regulated kinase (ERK), c-jun-N-terminal kinase and p38 in the MAPK signaling pathway. Pretreatment with Pae also inhibited hepatocyte apoptosis by reducing the expression of caspases 3, 8, 9, and Bax, and increasing Bcl-2. In total, protective effects of Pae against LPS/ d -GalN-induced ALF in mice are attributed to its antioxidative effect, inflammatory suppression in NF-κB and MARK signaling pathways, and inhibition of hepatocyte apoptosis inhibition. Therefore, Pae can be a potential therapeutic agent in attenuating LPS/ d -GalN-induced ALF in the future.
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JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY
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2017
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IF 1.591
Tengfei Bian,Xiujuan Zhang,Gaohong He,Zhijun Duan,Chunxu Dong,Xiangcun Li,Nana Guo
Hepatic encephalopathy (HE) is a neuropsychiatric disorder caused by chronic and acute liver diseases. It is believed that ammonia played an important role on the pathogenesis of the disease. Herein, acid-loaded water-in-oil-in-water (W1/O/W2) multiple emulsions with over 95% encapsulation efficiency were prepared and used to absorb colonic ammonia. The study of citric acid release from W1 to W2 in bulk deionized water indicated that only 17% acid released in 8 hours, which proved the stability of the multiple emulsions and hence prevented the intestine from being irritated by acid burst release. In vitro, the W1/O/W2 emulsion could remove around 90% ammonia in 1.5 hours from either the 3 mmol/L ammonia solution or the artificial colonic fluid with 1 and 0.5 mmol/L ammonia without acidifying the artificial colonic fluid. In vivo, compared with lactulose, W1/O/W2 emulsions could efficiently reduce the blood ammonia to the similar level in the rat models with HE without increasing the water content in feces. All these results indicated a potential application of W1/O/W2 multiple emulsions for the treatment of HE.
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Antioxidants
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2024
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IF 6
Xiao Xiao,Feiyu Yang,Yuling Huang,Shaohui Liu,Zhenhua Hu,Shanggao Liao,Yuanyuan Li
Numerous approaches targeting hepatic stellate cells (HSCs) have emerged as pivotal therapeutic strategies to mitigate liver fibrosis and are currently undergoing clinical trials. The investigation of herbal drugs or isolated natural active compounds is particularly valuable, due to their multifaceted functions and low risk of side effects. Recent studies have hinted at the potential efficacy of verbascoside (VB) in ameliorating renal and lung fibrosis, yet its impact on hepatic fibrosis remains to be elucidated. This study aims to evaluate the potential effects of VB on liver fibrosis by assessing its ability to inhibit HSC activation. VB demonstrated significant efficacy in suppressing the expression of fibrogenic genes in activated LX-2 cells. Additionally, VB inhibited the migration and proliferation of these activated HSCs by scavenging reactive oxygen species (ROS) and downregulating the AMPK pathway. Furthermore, a biosafe reverse microemulsion loaded with VB (VB-ME) was developed to improve VB’s instability and low bioavailability. The optimal formulation of VB-ME was meticulously characterized, revealing substantial enhancements in cellular uptake, ROS-scavenging capacity, and the suppression of HSC activation.
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ACS ES&T Engineering
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2024
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IF 7.4
Yi Shen,Mingzheng Yang,Chao Zhu,Haizhong Zhang,Renlan Liu,Jun Wang,Qile Fang,Shuang Song,Baoliang Chen
The activation of peroxymonosulfate (PMS) to generate singlet oxygen (1O2) for the removal of emerging organic pollutants (EOPs) from complex aqueous environments has garnered widespread attention. However, the low efficiency and selectivity of current PMS activation for 1O2 generation result in suboptimal EOP degradation. To enhance the selectivity of PMS activation and promote the non-radical pathway, non-metal heteroatoms with varying electronegativities were introduced to disrupt the symmetrical coordination structure of Fe active sites in Fe single-atom catalysts. The results showed that, in the B-Fe1/GLCNs/PMS system, the pseudo-first-order kinetic rate for bisphenol A (BPA) degradation reached 4.435 min–1, which is 7.4 times higher than that of the unmodified control group. Experimental and theoretical calculations demonstrated that the doping of non-metal heteroatoms altered the electron density and distribution at the Fe active sites, thereby modulating the adsorption configuration of HSO5– and increasing the selectivity for PMS activation to generate 1O2. Additionally, the degradation of EOPs by 1O2 produced intermediate products with lower biological toxicity, and 1O2 demonstrated strong anti-interference capability. The change in HSO5– morphology improved the rate of 1O2 generation. This study provides deep insights into designing high-performance PMS activation catalysts via non-metal doping to regulate the electronic structure of active sites for a selective non-radical pathway.
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ACS Nano
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2024
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IF 15.8
Kuo Wang,Yajie Luo,Hongtu Zhan,Xiao-Xia Liu,Xiaoqi Sun
Zn metal anodes experience dendritic growth and hydrogen evolution reactions (HER) in aqueous batteries. Herein, we propose an interface regulation strategy with a trace (1.4 × 10–4 mol kg–1) all-in-one epicatechin (EC) electrolyte additive to solve the above issues and reveal the roles of individual functional groups. By the disassembly of EC into simple molecules combined with entire molecule investigations, we show that phenol and ether sites preferentially anchor on the Zn surface, while the hydroxyl group pointing outward enters Zn2+ solvation shells at the interface. It modifies the following desolvation path, which not only enables uniform deposition with the thermodynamically favored plate morphology but also inhibits HER. With these synergistic effects of trace EC additive, the lifespan of symmetric cells extends to 8.5 times that of the baseline ZnSO4 electrolyte. The capacity retention of Zn//MnO2 full batteries with N/P = 3 also increases from 59.1 to 85.6% after 500 cycles.
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CHEMICAL ENGINEERING JOURNAL
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2024
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IF 13.3
Chong Zhang,Xiaoyi Jiang,Shuai Zhang,Xian Cao,Hui Wang,Takashi Sakamaki,Xianning Li
The polycyclic aromatic hydrocarbons (PAHs) frequently accumulate in sediments, particularly those near industrial sites. The efficacy of microbial electrochemical systems (MESs) in electricity generation and PAHs degradation is intricately linked to the microbiome’s response to high contaminant levels. Metabolomics and metagenomics were applied to elucidate the impact of a high pyrene concentration on the functional structure and metabolic dynamics of sediment microbiomes within MES. In sediment with a high pyrene concentration, both the electrical output of MES and efficiency of their microbiomes in degrading organic substrates and pyrene were markedly reduced. These conditions were associated with the enrichment of PAH-degrading microorganisms, such as Hydrogenophaga and Flavobacterium . Analyses of metabolites and genetic profiles revealed that metabolic pathways were primarily influenced in MES exposed to low pyrene concentrations whereas fewer metabolic pathways were activated in MES exposed to high concentrations; instead, pathways representing functions critical for microbial survival and communication were activated as well. According to enzymatic analyses, low pyrene levels promoted carbohydrate metabolism by sediment microorganisms, whereas high levels had an inhibitory effect. An examination of electron transport enzymes revealed that intracellular electron transfer was facilitated by both low and high concentrations. However, high levels impeded enzymes associated with outer membrane electron transfer, hindering electron movement from intracellular to extracellular electron acceptors and thus negatively affecting the electrical output of MES. Our study provides crucial theoretical insight and mechanistic understanding for addressing the challenges that may be encountered when MES are used to treat pyrene-contaminated sediment near industrial sites.
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JOURNAL OF BIOLOGICAL CHEMISTRY
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2024
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IF 4
Chengmeng Jin,Wei Hu,Yuqi Wang,Hao Wu,Siying Zeng,Minfeng Ying,Xun Hu
Most cancer cells exhibit high glycolysis rates under conditions of abundant oxygen. Maintaining a stable glycolytic rate is critical for cancer cell growth as it ensures sufficient conversion of glucose carbons to energy, biosynthesis, and redox balance. Here we deciphered the interaction between PKM2 and the thermodynamic properties of the glycolytic pathway. Knocking down or knocking out PKM2 induced a thermodynamic equilibration in the glycolytic pathway, characterized by the reciprocal changes of the Gibbs free energy (ΔG) of the reactions catalyzed by PFK1 and PK, leading to a less exergonic PFK1-catalyzed reaction and a more exergonic PK-catalyzed reaction. The changes of the ΔGs of the two reactions causes the accumulation of intermediates, including the substrate PEP (the substrate of PK), in the segment between PFK1 and PK. The increased concentration of PEP in turn increased PK activity in the glycolytic pathway. Thus, the interaction between PKM2 and the thermodynamic properties of the glycolytic pathway maintains the reciprocal relationship between PK concentration and its substrate PEP concentration, by which, PK activity in the glycolytic pathway can be stabilized and effectively counteracts the effect of PKM2 KD or KO on glycolytic rate. In line with our previous reports, this study further validates the roles of the thermodynamics of the glycolytic pathway in stabilizing glycolysis in cancer cells. Deciphering the interaction between glycolytic enzymes and the thermodynamics of the glycolytic pathway will promote a better understanding of the flux control of glycolysis in cancer cells.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2024
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IF 9.4
Yue Chen,Shaohua Zhang,Dongni Zhao,Zhixian You,Yubiao Niu,Liqiang Zeng,Nagarathinam Mangayarkarasi,Oleg V. Kolosov,Jianming Tao,Jiaxin Li,Yingbin Lin,Yongping Zheng,Long Zhang,Zhigao Huang
The widespread application of Li 4 Ti 5 O 12 (LTO) anode in lithium-ion batteries has been hindered by its relatively low energy density. In this study, we investigated the capacity enhancement mechanism of LTO anode through the incorporation of Na + cations in an Li + -based electrolyte (dual-cation electrolyte). LTO thin film electrodes were prepared as conductive additive-free and binder-free model electrodes. Electrochemical performance assessments revealed that the dual-cation electrolyte boosts the reversible capacity of the LTO thin film electrode, attributable to the additional pseudocapacitance and intercalation of Na + into the LTO lattice. Operando Raman spectroscopy validated the insertion of Li + /Na + cations into the LTO thin film electrode, and the cation migration kinetics were confirmed by ab initio molecular dynamic (AIMD) simulation and electrochemical impedance spectroscopy, which revealed that the incorporation of Na + reduces the activation energy of cation diffusion within the LTO lattice and improves the rate performance of LTO thin film electrodes in the dual-cation electrolyte. Furthermore, the interfacial charge transfer resistance in the dual-cation electrolyte, associated with ion de-solvation processes and traversal of the cations in the solid-electrolyte interphase (SEI) layer, are evaluated using the distribution of relaxation time, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. Our approach of performance enhancement using dual-cation electrolytes can be extrapolated to other battery electrodes with sodium/lithium storage capabilities, presenting a novel avenue for the performance enhancement of lithium/sodium-ion batteries.
CAS / MDL:
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POLYMER
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2024
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IF 4.1
Zhenxian Chen,Yingying Wang,Zhao Li,Hui Zhang,Ming Wan,Nan Zheng,Bijin Xiong,Jintao Zhu
The cavitation behavior of polypropylene-silica (PP-silica) and high density polyethylene-silica (HDPE-silica) composites with varying silica content during uniaxial tension was investigated via in situ X-ray techniques. The introduction of silica was found to significantly influence the mechanical properties of the materials. Small-angle X-ray scattering (SAXS) was employed to determine the cavitation onset strain and to observe the evolution of cavity structures. Through model fitting, the size evolution of nanoscale cavities at the onset of cavitation was quantitatively characterized. Scanning electron microscopy (SEM) was utilized to examine the morphology before and after stretching, revealing that cavity structures primarily resulted from internal defects in the polymer matrix and interfacial debonding between silica and the polymer. The long axis of the nanoscale cavities at the onset of cavitation was perpendicular to the tensile direction, and further deformation led to the coalescence into micron-sized cavities with the long axis parallel to the tensile direction. Wide-angle X-ray scattering (WAXS) was used to characterize the microscale deformation process of crystals, showing that during stretching, the lamellae reoriented with the angle between their normal direction and the tensile direction gradually decreasing. Several sets of transition strains representing the plastic deformation were used to compare the microscale and macroscale deformation, elucidating the sequence and differentiation between crystal plastic deformation, cavitation, and yield in the two polymer materials. Intriguingly, both PP-silica and HDPE-silica composites exhibited optimal silica additions and initial structural parameters for regulating cavitation behavior. These findings offer significant guidance for the control of internal structures in porous separators in industrial production.
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Advanced Science
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2024
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IF 14.3
Dingyuan Guo,Nan Li,Xiaoyan Zhang,Runxin Zhou,Jie He,Xiao-Ping Ding,Weixing Yu,Fuqiang Tong,Sibi Yin,Yu Wang,Xin Xu,Long Wang,Mingzhu Fan,Shan Feng,Ke Liu,Ke Tang,Zhuqing Ouyang,Yusong R Guo,Yugang Wang
N 6 -acetyl- L - lysine residue is abundant in dietary protein but little is known about its potential influences on the diet-consumers. Herein, it is reported that Lysyl-tRNA synthetase (KARS) mediates co-translational deposition of diet-derived N 6 -acetyl- L -lysine (AcK) in nascent proteins to contribute to the acetylome in cells. Acetylated dietary protein is a direct source of AcK that can widely and substantially regulate the acetylome in multiple organs of mice. By analyzing the mechanisms underlying AcK contributing to the acetylome in mammalian cells, it is found that KARS can utilize AcK as an alternative substrate to produce N 6 -acetyl- l -lysyl-tRNA. The crystal structure of KARS in complex with AcK at 2.26 Å resolution shows that AcK shares the same substrate-binding pocket as L -lysine, allowed by a sidechain flip of Tyr499. The generated N 6 -acetyl- L -lysyl-tRNA introduces AcK into growing nascent polypeptide and results in protein acetylation, including the regions buried inside folded proteins that are post-translational modification (PTM)-inaccessible and functionally important. This undocumented protein modification mechanism is inherently different from PTM and termed as co-translational modification (coTM). It is expected to extend the repertoire of acetylome and improve the understanding of protein modification mechanisms in cells.
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CHEMOSPHERE
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2024
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IF 8.1
Huilin Chen,Maomao Xie,Wenjie Li,Lipin Tan,Xiaoming Cai,Mingjing Shen,Ruibin Li
The extensive use of synthetic antioxidants, notably N-(1,3-dimethylbutyl)-N′-phenyl- p -phenylenediamine (6-PPD), in rubber-related products, particularly in tire manufacturing, has induced concerns regarding their environmental impact and potential health hazards. Despite the identification of 6-PPD and its derivative, 6-PPD quinone (6-PPDQ), in various water samples and their lethal effects on certain aquatic species ( e.g. , coho salmon, rainbow trout and brook trout), the levels of airborne 6-PPD/6-PPDQ and their respiratory toxicity remain relatively unexplored. In this study, we aimed to evaluate the respiratory toxicity potential of 6-PPD and its derivatives, with a specific focus on detecting these compounds in airborne particulates and assessing their toxic effects on lung cells. Characterization of four airborne fine particulate (FP) samples revealed spherical morphologies with diameters ranging from 17.7 to 225.7 nm, displaying slight agglomeration and negative surface charge. methanol/acetonitrile extraction followed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) analysis confirmed the presence of both 6-PPD and 6-PPDQ on the surfaces of FPs, with significant variations (0.26–1.05 μg g −1 ) in loading capacity observed among the samples. Subsequent exposure of lung cells (THP-1, BEAS-2B, and A549) to 6-PPD and 6-PPDQ revealed dose-dependent declines in mitochondrial metabolic activity induced by 6-PPD, along with severe membrane damage, ATP depletion, and pro-inflammatory cytokine release. Conversely, 6-PPDQ exhibited negligible toxicity in all tested parameters. These findings underscore the potential health risks associated with airborne 6-PPD exposure and emphasize the importance of further research into the respiratory toxicity of 6-PPD derivatives.
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危险品化学品经营许可证（带存储）