COLLOIDS AND SURFACES B-BIOINTERFACES
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2026
·
IF 5.9
Wenjie Zhang,Lingxin Zeng,Ying Li,Xinggang Yang,Wei Xiong
Phosphatidylserine liposomes target injured renal tubules. • Spermine-modified chitosan enables responsive drug release. • Targeted dihydroartemisinin delivery restores Klotho and reduces fibrosis. Restoration of endogenous Klotho represents a promising strategy for suppressing renal fibrosis, but efficient delivery of Klotho-restoring therapeutics to injured renal tubules remains challenging. Here, we developed dihydroartemisinin (DHA)-loaded phosphatidylserine liposomes coated with spermine-modified chitosan (DHA-PSLPs@SC) to achieve sequential kidney injury molecule-1 (KIM-1) targeting and spermine oxidase (SMOX)-responsive release, thereby enhancing renal delivery of DHA to restore endogenous Klotho and attenuate renal fibrosis. Dioleoylphosphatidylserine (DOPS) was incorporated to facilitate uptake by KIM-1-expressing injured tubular epithelial cells, whereas the spermine-modified chitosan (SC) coating was designed to promote cargo release in the SMOX-enriched fibrotic cellular environment. The optimized DHA-PSLPs@SC exhibited a diameter of 102.3 nm, a zeta potential of 33.8 mV, and an encapsulation efficiency of 69.4%. In H₂O₂-injured HK-2 cells, DOPS-containing liposomes displayed enhanced uptake and colocalization with KIM-1, while KIM-1 antibody blockade markedly reduced their internalization. In TGF-β1-stimulated NRK-52E cells, the SC coating preserved cellular uptake and increased cumulative DHA release to 74.2% at 12 h, while DHA-PSLPs@SC more effectively restored Klotho and suppressed fibrotic markers than free DHA or non-targeted liposomes. In unilateral ureteral obstruction (UUO) mice, PSLPs@SC preferentially accumulated in obstructed kidneys, with approximately 2.7-fold higher renal accumulation than non-targeted liposomes at 2 h. DHA-PSLPs@SC improved renal function, reduced tubular injury and collagen deposition, restored renal Klotho, and suppressed fibrosis. These findings support sequential KIM-1 targeting and SMOX-responsive release as a strategy to enhance renal delivery and antifibrotic efficacy of DHA, restore endogenous Klotho, and attenuate renal fibrosis.
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Journal of Integrative Neuroscience
·
2026
·Vol. 25
·期 5
·
IF 3.3
Hui Cai,Xinran Xu,Xuerui Zhuo,Zhe Liu,Hao Liu,Yiming Sun
Background:Epilepsy is a central nervous system disorder characterized by abnormal neuronal discharges in the brain. The purpose of this study was to investigate the protective effects of a caspase-1 inhibitor on glial hyperactivation and neuronal apoptosis in epilepsy.Methods:A pilocarpine-induced status epilepticus (SE) mouse model was established. Belnacasan (VX765), a caspase-1 inhibitor, was administered intraperitoneally. ELISA was used to detect inflammatory cytokines interleukin (IL)-1β, IL-6, and tumor necrosis factor-alpha (TNF-α) in peripheral blood. Immunohistochemistry and transmission electron microscopy were used to evaluate glial activation and neuronal damage in the hippocampus. Western blotting was performed to detect caspase-1 and Gasdermin D N-terminal (GSDMD-N) expression. In vitro, primary glial cells were stimulated with lipopolysaccharide (LPS), and the effects of glial conditioned medium on HT22 neuronal apoptosis were assessed using cell counting kit-8 (CCK-8) and flow cytometry. Results:In vivo experiments showed that, as epilepsy progressed, the levels of pro-inflammatory cytokines IL-1β and IL-6 in peripheral blood were significantly increased, consistent with findings in patients with epilepsy. At 21 days after epilepsy induction, the numbers of hyperactivated microglia and astrocytes increased significantly and exhibited activation-related features such as organelle swelling, whereas neuronal numbers were markedly reduced and displayed cytological features of apoptosis. VX765 significantly alleviated seizure frequency and severity in epileptic mice and attenuated peripheral blood levels of IL-1β and IL-6, hippocampal caspase-1 activity, glial hyperactivation, and neuronal apoptosis. In vitro experiments demonstrated that glial conditioned medium (CMG) promoted apoptosis of HT22 neurons by regulating Bcl-2-associated X protein (Bax) and B-cell lymphoma 2 (Bcl-2) expression, whereas VX765 alleviated HT22 neuronal apoptosis by inhibiting the secretion of inflammatory factors from glial cells.Conclusions:These results indicate that inhibiting glial cell hyperactivation and neuroinflammation via caspase-1 inhibition may represent a potential therapeutic strategy for epilepsy.
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REDOX REPORT
·
2026
·
IF 6.9
Dongcen Lang,Yanning Zheng,Jing Zhang,Zheng Li,Mengyu Niu,Jian Wang,Xue Wang,Liyong Huang,Jiangyu Xue,Li Li,Ying Chen
Objective Subarachnoid hemorrhage (SAH) is associated with high mortality and poor outcomes, which are closely related to white matter injury (WMI). (-)-Epigallocatechin-3-gallate (EGCG) exerts neuroprotective effects by inhibiting oxidative stress-related ferroptosis in astrocytes and improving neurological function. However, the role and mechanism of EGCG in regulating reactive astrocytes (RAs) to alleviate WMI after SAH remain unclear.Methods A mouse model of SAH was used to evaluate the effects of EGCG-loaded nanoparticles (EGCG-NPs). Western blot, qPCR, and immunofluorescence were performed for biochemical analysis. Neurological function was assessed using neurological deficit scores and the Morris water maze test.Results Following SAH, HO-1-mediated iron accumulation and ROS production promoted A1/A2 reactive astrocyte polarization, resulting in myelination damage and aggravated WMI. Knockdown of S100A10 inhibited iron-dependent oxidative stress in RAs and attenuated WMI. EGCG-NPs significantly suppressed HO-1/S100A10-mediated iron overload and oxidative stress in both A1 and A2 RAs, thereby alleviating WMI after SAH.Conclusion EGCG-NPs attenuate SAH-induced WMI by inhibiting the iron overload-activated HO-1/S100A10 axis in RAs, representing a promising therapeutic strategy.
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Journal of Fungi
·
2026
·Vol. 12
·期 2
·157
·
IF 4.3
Yajing Yin,Hanxing Zhang,Zhenying Zhang,Mi Zhou,Shaojie Li,Chengcheng Hu
Fusarium verticillioides is a significant agricultural pathogen and an emerging causative agent of invasive fusariosis in clinical settings. Fusarium species frequently exhibit resistance to available antifungal agents, yet the molecular mechanisms underlying azole resistance remain poorly characterized. In this study, we identified the Zn(II) 2 Cys 6 transcription factor FvADS-1 as a positive regulator of the azole stress response in F. verticillioides . The transcription of FvADS-1 was significantly induced by ketoconazole (KTC), and its deletion increased susceptibility to multiple azole compounds. Mechanistically, FvADS-1 positively regulates the KTC-induced expression of genes encoding ABC transporters and ergosterol biosynthesis enzymes, thereby modulating intracellular KTC accumulation and sterol homeostasis under azole stress. Furthermore, FvADS-1 positively regulates the transcriptional response of peroxisomal genes and contributes to fungal tolerance to oxidative stress. Notably, deletion of FvADS-1 attenuates the virulence of F. verticillioides on maize. The function of ADS-1 is evolutionarily conserved: heterologous expression of N. crassa ads-1 restored azole resistance in FvADS-1 deletion mutant, and the deletion of the F. oxysporum homolog FoADS-1 similarly increased azole susceptibility. Collectively, our study demonstrates that the conserved transcription factor ADS-1 plays a central role in regulating azole resistance and virulence in the pathogen F. verticillioides , offering new insights into antifungal resistance mechanisms in pathogenic filamentous fungi.
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Food Chemistry-X
·
2026
·
IF 9.2
Wen Zeng,Zhiwen Zeng,Tao Wang,Yacong Hou,Youfeng Jiang,Dafeng Dong,Tianxiang Li,Lufeng Fu,Ying Liu,Xuesong Cao,Zhuo Chen,Peitao Lü
The unique apple-like aroma of the ‘Fenza 1’ (FZ1) banana has long been valued by consumers, yet the underlying chemical basis remains poorly understood. Therefore, this study aimed to investigate the specific compounds and mechanisms behind this distinctive aroma phenotype using a multi-faceted approach. Descriptive sensory analysis revealed that FZ1 displayed an apple-like aroma intensity intermediate between the common ‘Baxi’ (BX) banana and the ‘Ruixue’ (RX) apple. Headspace solid-phase microextraction gas chromatography–mass spectrometry (HS-SPME-GC–MS) and gas chromatography-olfactometry (GC-O) analyses identified 17 aroma-active compounds contributing to the aroma variations among the three fruits. Among these, hexyl acetate (OAV = 58), trans-2-hexenal (OAV = 502), and hexanal (OAV = 1191) were identified as key contributors to the apple-like aroma. Aroma addition experiments further demonstrated that these compounds, particularly in combination, enhanced the perception of apple-like aroma within a banana matrix. Molecular docking and molecular dynamics simulations suggested stable interactions between these compounds and specific human olfactory receptors via hydrophobic interactions and hydrogen bonding. These findings clarify the chemical basis of this distinctive aroma and can guide flavor enhancement strategies for bananas.
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Surfaces and Interfaces
·
2026
·
IF 6
Mohamed M. Abdel-Halim,Zhongyu Wang,Zhenyi Hu,Xuesong Jiang,A. Shahboub,Zongrong Zhou,Boyuan Ban,Jian Chen
A new collector, N-Oleyl-1, 3-propanediamine (NOPDA), has strong collecting power and excellent selectivity. • NOPDA achieves effective separation of mica and quartz using a low dosage a 0.016 mmol/L at pH 2.5. • NOPDA preferentially adsorbs onto mica surfaces through hydrogen bonding and electrostatic interactions. • NOPDA is demonstrated as a new flotation collector for the mica surface with high-efficiency separation. The selective separation of mica from quartz remains a significant challenge because of their similar physicochemical properties. In this study, a cationic diamine collector, N-oleyl-1,3-propanediamine (NOPDA), was evaluated for the flotation separation of mica from quartz under acidic conditions. Single-mineral flotation results demonstrated a substantial recovery difference of approximately 84%, with mica recovery reaching 95%, whereas quartz recovery remained low (10.91%) at pH 2.5, indicating a strong selectivity. Mixed-mineral flotation further confirmed effective separation, yielding a concentrate enriched in mica-associated components, including Al 2 O 3 (33.05%, 94.41% recovery) and K 2 O (11.39%, 98.46% recovery), with a relatively low SiO 2 recovery (32.50%). The flotation mechanisms were analyzed through zeta potential testing, contact angle, zeta potential, FTIR, and XPS analyses, which revealed that NOPDA preferentially adsorbs on mica surfaces, significantly enhancing hydrophobicity, whereas only weak interactions were observed on quartz. The adsorption mechanism is primarily attributed to the electrostatic attraction and hydrogen bonding of the protonated amine species with the surface Al–O and Si–O sites of the adsorbent. Density Functional Theory (DFT) calculations indicated that the adsorption energy difference between NOPDA on mica and quartz was 2.75 eV, which is consistent with the flotation results. Overall, these findings demonstrate that NOPDA exhibits promising selective flotation performance for mica–quartz separation under acidic conditions. The combined flotation experiments, surface characterization, and DFT analysis provide insights into the preferential adsorption behavior of NOPDA on the mica surface. Download: Download high-res image (175KB) Download: Download full-size image
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2026
·
IF 5.4
Renfan Chen,Kaile Zhao,Weilong Wu,Kunxi Huang,Mengyao Zhou,Xiaolong Huang,Bing Deng,Zhen Wang,Wenpu Zhang
Efficient flotation separation of pentlandite from pyrite is central to improving the utilization efficiency of nickel-cobalt sulfide resources. In this work, 3-mercapto-1-propanol (3-MP) was employed as a selective pyrite depressant in Ni-Co sulfide flotation. The selective depression mechanism was elucidated through single-mineral, artificial mixed-mineral and real ore flotation tests, combined with contact angle measurements, adsorption measurements, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy characterizations. Single-mineral flotation results demonstrated that 3-MP reduced pyrite recovery to 19% while maintaining pentlandite recovery steadily at 75%. In artificial mixed-mineral flotation, 3-MP significantly upgraded the nickel concentrate grade, with a nickel recovery of 85.42%. Open-circuit flotation (one rougher, one scavenger and two cleaner stages) on a real ore yielded a nickel concentrate assaying 3.40% Ni and 1.73% Co, corresponding to enrichment ratios of 5.7-fold and 5.6-fold, respectively, and the sulfur recovery to the concentrate was merely 10.07%, indicating favorable practical application potential of this reagent. Surface analyses confirmed that 3-MP chemically bonds with Fe sites on the pyrite surface via its thiol group (–SH), while exhibiting negligible interaction with Ni sites on pentlandite, thereby leaving the surface properties and collector adsorption behavior of pentlandite unaffected and ensuring its excellent floatability. This work provides a theoretical basis and technical support for the efficient separation and utilization of nickel-cobalt sulfide resources. Download: Download high-res image (176KB) Download: Download full-size image
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PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
·
2026
·
IF 7.9
Xin Qi,Ziwei Wang,Qixin Xia,Xiuping Liang,Guiyin Wang,Changlian Xu,Guochun Lv,Junzhuo Cai,Zhang Cheng,Zhanbiao Yang,Liling Wang,Xiaoxun Xu,Hua Yin
Although microbial degradation has been extensively studied for removing 2,2′,4,4′-tetrabrominated diphenyl ether (BDE-47), knowledge regarding the comparative efficiency and safety of degradation by facultative anaerobes under varying oxygen conditions remain limited. This study investigated the degradation of BDE-47 by Pseudomonas sp. strain QS-1 under both aerobic and anaerobic conditions. Our findings revealed that 47.5% and 12.4% of the initially added BDE-47 (0.5 mg/L) were transformed within 7 days under aerobic and anaerobic conditions, respectively. The aerobic transformation involved a complex pathway consisting of debromination, hydroxylation, ether bond cleavage, and ring opening, whereas the anaerobic transformation was limited to debromination. Toxicity assessments using an Escherichia coli model revealed that aerobic degradation products reduced cytotoxicity, whereas anaerobic degradation products induced higher rates of cell apoptosis than the parent BDE-47. Metabolomics analysis attributed this enhanced toxicity of anaerobic products to the specific inhibition of purine metabolism. In soil microcosm experiments, strain QS-1 exhibited stronger colonization and degradation performance under aerobic conditions, shifting the soil microbial community assembly towards more deterministic processes and strengthening network interactions with indigenous taxa. These findings provide insights into the biological effects of BDE-47 degradation products, and inform the assessment of efficacy and safety for bioremediation strategy. Download: Download high-res image (175KB) Download: Download full-size image
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Genes
·
2026
·Vol. 17
·期 5
·586
·
IF 3.1
Jiayi Ying,Zhishuang Wang,Yinuo Shen,Yangdeng Lin,Yao Wang,Congying Zhu,Yiyang Xu,Luanfang Lin,Xiangtao Zhu,Xia Chen,Qianqian Wang
Background/Objectives: Tree peony (Paeonia suffruticosa) has a short flowering period, and its regulatory mechanisms remain poorly understood. These limitations have severely constrained its industrial development. Expansins (EXPs) are key proteins that mediate cell wall loosening associated with petal expansion, yet they remain uncharacterized in tree peony. Methods: This study utilized gene family identification, key gene screening and functional research, as well as regulatory network analysis to reveal the role of the EXP family in the regulation of flower opening. Results: This study presents the first genome-wide identification of 36PsEXPgenes in tree peony, classifying them into four evolutionarily conserved subfamilies (PsEXPA, PsEXPB, PsEXLA, and PsEXLB). Promoter analysis revealed that 28 out of 36PsEXPpromoters contain gibberellin (GA)-responsive elements. Exogenous GA3treatment significantly promoted flower opening and upregulated eightPsEXPs, withPsEXPA4-1showing the highest expression level and promoter GA-responsive element abundance. Subcellular localization confirmed that PsEXPA4-1 was targeted to the cell wall. Overexpression ofPsEXPA4-1inArabidopsisled to early flowering and enlarged petals, indicating thatPsEXPA4-1had the potential to promote cell expansion, consistent with its proposed role in tree peony flower opening. Mechanistically, we identified a bHLH transcription factor, PsbHLH25, whose expression was induced by GA. Y1H and dual-luciferase assays indicated that PsbHLH25 can bind to thePsEXPA4-1promoter. Conclusions: This study systematically characterized the EXP gene family in tree peony, revealedPsEXPA4-1as a key effector downstream of GA promoting flower opening, and discoveredPsbHLH25as a transcriptional activator linking GA signaling toPsEXPA4-1. These findings provided important insights into GA-mediated floral opening and genetic resources for understanding the molecular mechanisms and enabling precise flowering time regulation in tree peony.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2026
·
IF 9.1
Shuxin Zhang,Kun Liu,Shuyuan Yu,Zifeng Lin,Yu Xie,Yanbin Jiang,Longfu Wei,Changlin Yu
Traditional propylene oxide (PO) production processes consume large amounts of organic feedstock and release hazardous by-products. The synthesis of PO via the epoxidation of propylene using O 2 is an ideal route offering 100% atomic economy. However, achieving high selectivity and high efficiency under mild conditions remains a significant challenge. In this study, we have developed a visible-light-driven tandem reaction system combining a carbon- and oxygen-co-doped three-dimensional spherical indium sulfide (C,O-In 2 S 3 ) photocatalyst with titanium silicate molecular sieve (TS-1), thereby achieving the distinctly selective oxidation of propylene at ambient temperature. Assembled from ultra-thin nanosheets, the three-dimensional structure of C,O-In 2 S 3 provides abundant active sites and mass transfer space, with carbon‑oxygen co-doping narrowing the bandgap and enhancing visible light absorption. In a pure water system, the H 2 O 2 yield of C,O-In 2 S 3 reached 1450.40 μmol·g −1 ·h −1 , which is 17.8 times that of pristine In 2 S 3 . Utilizing the excellent compatibility of the methanol/water system, the photocatalytic generated H 2 O 2 was directly employed in the TS-1-catalysed epoxidation of propylene, achieving a PO yield of 2230.03 μmol·g −1 within 2 h with a selectivity of 99.1%. This work applies sulfide-based photocatalysts to a tandem reaction involving photocatalytic H 2 O 2 production and propylene epoxidation, providing a new strategy for the green, safe and efficient synthesis of PO.
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CHINESE JOURNAL OF POLYMER SCIENCE
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2026
·
IF 4.6
Jia Hui-Wen,Lv Xue,Ma Yan-Ming,Xu Wang,Yang Xiao-Ning
Conductive hydrogels with high mechanical strength, good adhesion, and high sensitivity are highly demanded for wearable electronics. A multifunctional hydrogel based on 2-acrylamido-2-methylpropanesulfonic acid (AMPS) and acrylic acid (AA) was synthesized via free-radical polymerization. The system incorporates dynamic hydrogen bonds from guanine–cytosine (G–C) base pairs and homogeneously dispersed carbon nanotubes (CNTs) modified using a dual-ionic liquid strategy. Short-chain (C 4 ) and long-chain (C 12 ) imidazole-based ionic liquids act synergistically to exfoliate and stabilize CNTs, facilitating uniform dispersion and strong interfacial bonding. Owing to the continuous conductive pathways formed by the CNTs, the hydrogel exhibited enhanced electrical conductivity with a gauge factor of 5.61, together with excellent short-term cyclic stability and responsiveness for monitoring dynamic human motions. The combination of covalent cross-linking, reversible hydrogen bonding, and reinforced nanofillers endows the hydrogel with high stretchability, good adhesion (5.5 kPa), and superior fatigue resistance, showing great potential for use in flexible wearable bioelectronics.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·Vol. 74
·期 30
·23674-23683
·
IF 6.7
Xu Meng,Liu Han,Lin Guangyu,Jing Zijian,Wen Tao,Xue Weijie,Wang Hualei,Wei Dongzhi
Transaminases
provide an effective approach for the asymmetric
synthesis of chiral amines. However, native ω-transaminases
often suffer from low catalytic efficiency and poor substrate concentration
tolerance, limiting industrial applicability. Targeting the synthesis
of ( S )-1-Boc-3-aminopiperidine, this study engineered
the ( S )-selective ω-transaminase CC1012 from Caulobacter sp. D5 through active pocket remodeling and
substrate tunnel engineering. Hydrophobic remodeling of the small
binding pocket yielded the single mutant M1 (Q25F) with a 27-fold
improvement in intrinsic catalytic efficiency ( k cat / K m ). Conservation analysis
and virtual saturation mutagenesis further identified R424 and D320
as key residues in the loop region at the substrate tunnel entrance.
Iterative saturation mutagenesis on these sites using M1 as a template
produced the optimal mutant M3 (Q25F/D320M/R424L), exhibiting a 45-fold
enhancement in k cat / K m over the wild type, achieving 98% conversion with ee > 99.9% at 1000 mM substrate concentration. This study
provides a superior biocatalyst for the efficient synthesis of ( S )-1-Boc-3-aminopiperidine.
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