PLANT CELL REPORTS
·
2026
·Vol. 45
·期 10
·321
·
IF 5.3
Yan Xiao,Rao Mingxiang,Tao Yu,Ran Weixi,Wang Yuchun,Lv Wuyun,Ren Hengze,Chen Yanan,Lu Mengqian,Jing Tingting,Li Chunfang
Key message CsWRKY22 acts as a positive regulator of CsFLSd through binding to proCsFLSd, promoting quercetin accumulation in tea plants, which contributes to the astringent taste of tea. Flavonol derivatives, including quercetin, are nutritionally valuable dietary components with health benefits. In tea plants, their high accumulation not only contributes to the beverage’s nutritional quality but also influences its characteristic astringent taste. To gain a deeper insight into the mechanisms underlying flavonols biosynthesis in tea plants, this study first identified a candidate flavonol synthase-encoding gene, CsFLSd , via bioinformatic mining of the tea plant transcriptome. Functional validation showed that targeted manipulation of CsFLSd expression directly modulated quercetin levels: transient suppression of CsFLSd expression in tea plant leaves resulted in a significant reduction in quercetin content. Heterologous stable overexpression of CsFLSd in Arabidopsis thaliana further confirmed its role in promoting quercetin accumulation. To dissect the upstream regulation of CsFLSd , a Yeast One-Hybrid (Y1H) screen was performed using a tea plant cDNA library, leading to the identification of CsWRKY22 as a potential interacting partner of the CsFLSd promoter. Subsequent validation experiments revealed that CsWRKY22 could specifically bind to the CsFLSd promoter. Functional analyses demonstrated that transient overexpression of CsWRKY22 in tea plant leaves elevated the quercetin content, whereas transient suppression of its expression reduced quercetin accumulation, indicating that CsWRKY22 acts as a positive regulator of quercetin biosynthesis. Collectively, these findings characterize an important regulatory step in quercetin biosynthesis in tea plants, defined by the CsWRKY22 -proCsFLSd module that controls quercetin production. Additionally, this study provides valuable insights into flavonols biosynthesis in tea plants, advancing the understanding of flavonoid metabolic regulation in tea plants.
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JOM
·
2026
·
IF 2.3
Tian Zhiqiang,Wang Yu,Xu A-min,Chen Xinjie,Zhang Ting-An
This study systematically investigates the viscosity behavior of a potassium cryolite-based electrolyte (KF-AlF 3 ) over the temperature range of 450–800°C. Compared with previous measurements conducted at selected temperatures and cryolite ratios (CR), this work extends the viscosity characterization of potassium cryolite-based electrolytes across a wide temperature range covering both the practical electrolysis window and the solid–liquid coexistence region, while providing systematic insight into the effects of CR and Al 2 O 3 content. High-resolution viscosity–temperature data were obtained under controlled conditions with CR between 1.2 and 1.5 and Al 2 O 3 concentrations from 0 wt.% to 5 wt.%. The results indicate that viscosity is dominantly governed by the CR, while temperature and Al 2 O 3 content exert comparatively weaker effects. On average, viscosity increases by approximately 2.7% for every 0.1 wt.% increase in CR and by approximately 1.2% for each 1 wt.% increase in Al 2 O 3 content. Two distinct viscosity inflection points were observed in the 480–500°C interval. The primary inflection near 500°C corresponds to the onset of K 3 AlF 6 crystallization, whereas the secondary inflection around 480°C is tentatively attributed to enhanced interactions between Al 2 O 3 -derived solid particles during further cooling. A segmented Arrhenius-type model was established, providing a unified viscosity description across the investigated temperature and composition ranges with a mean relative deviation below 1.5%. The model parameters reveal a substantially higher activation energy in the low-temperature segment associated with solid–liquid coexistence, while the high-temperature regime exhibits mild viscosity variation characteristic of ionic-liquid-like behavior. This model is directly applicable to CFD-DEM coupled simulations and low-temperature aluminum electrolysis process optimization.
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MEDICAL ONCOLOGY
·
2025
·Vol. 42
·期 8
·1-13
·
IF 3.5
Cai Zhiren,Xie Yanqi,Li Luyao,Zheng Yuhang,Zhou Xianghui,Zhou Guanghua,Wang Ganping,Zeng Xin
Bladder cancer is a major health concern, and understanding its molecular mechanisms is essential for developing effective therapies. TET3, a DNA hydroxymethylase, has been linked to tumor progression, but its role in bladder cancer remains unclear. We analyzed single-cell RNA sequencing data and identified TET3 as a key regulator in tumor-promoting cells using scissor analysis. Functional experiments were performed to evaluate the effects of TET3 knockdown on cell proliferation, migration, and gene expression. In vivo tumor growth assays and histological analyses were conducted to assess the role of TET3 in tumor progression. Additionally, we assessed the impact of TET3 on stemness-associated gene expression and performed sphere formation assays to evaluate tumor cell self-renewal capacity. TET3 was highly expressed in tumor-promoting cells. Its knockdown significantly reduced cell proliferation, migration, and tumor growth in vivo. Tumors with TET3 knockdown had lower volumes, weights, and Ki67-positive cells. Mechanistically, TET3 regulated key tumor-related genes and pathways and reduced DNA 5hmC levels. Notably, TET3 knockdown downregulated multiple stemness-related transcription factors such as SOX2 and NANOG, and impaired sphere-forming efficiency, suggesting its essential role in maintaining cancer cell stemness. This study reveals that TET3 promotes tumor growth and progression in bladder cancer partly through modulation of the stemness pathway, highlighting its potential as a therapeutic target and prognostic marker.
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高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 7
·1-14
·
IF 5.3
Zou Na,Li Xin,Xu Meiling,Wang Zhaoxia,Zhang Junhua,Wang Xueliang
In the direct electrochemical sensing of endogenous hydrogen sulfide, the utilization of triple-pulse amperometry (TPA) enables the delivery of distinct cleaning and measurement pulses, effectively mitigating electrode surface passivation due to sulfur deposition. In order to further improve the sensor’s sensitivity and selectivity, gold nanoparticles(nano-Au), platinum nanoparticles (nano-Pt), and poly(3,4-ethylenedioxythiophene) (PEDOT) were separately employed to modify electrodes for constructing electrochemical sensors. Their performance in detecting hydrogen sulfide was evaluated using constant potential amperometry (CPA) alongside TPA. Selectivity coefficients were determined based on current responses to hydrogen sulfide as well as four major interfering substances: ascorbic acid (AA), dopamine (DA), uric acid (UA), and epinephrine (EP). After quantitative comparison, the optimal solution for direct electrochemical sensing of hydrogen sulfide involved employing a PEDOT/nano-Au composite film in conjunction with TPA technology. The sensor responds to hydrogen sulfide in the concentration range 3.0–24.0 µM with a detection limit of 0.035 µM. Furthermore, the sensor demonstrates excellent repeatability and stability, rendering it suitable for continuous electrochemical monitoring of hydrogen sulfide in simulated real biological environments. Graphical
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高影响因子
CHEMICAL PAPERS
·
2025
·
IF 2.5
Gu Peng
Dielectric composites with high dielectric constant, minimal dielectric loss and low conductivity can well serve for energy storage capacitors. High dielectric loss is not favorable due to large energy waste. Here, to improve dielectric constant of poly(vinylidene fluoride–hexafluoropropylene) by electric percolation, semiconducting NiO filler was added to form composites. However, high leakage current from interfaces can lead to high dielectric loss. Doping insulating α-Al 2 O 3 particles with stable structure, low chemical activity and high modulus into above binary composites can reduce dielectric loss. By changing NiO dose, dielectric constant was well tailored. Dose of α-Al 2 O 3 was left unchanged. Dielectric loss, conductivity and dielectric constant of composites were measured. By the same NiO loading, dielectric loss, conductivity and dielectric constant in ternary composites can be reduced, compared with binary composites. The optimal ternary composite should contain 35 wt% NiO and 4 wt% α-Al 2 O 3 . Optimized dielectric traits of this composite can be reflected by dielectric constant of ~ 64 and dielectric loss of ~ 0.27 under 100 Hz electric field.
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TRANSACTIONS OF TIANJIN UNIVERSITY
·
2025
·
IF 8.5
Huang Xiaorui,Zhang Wei,Xiao Liyang,Han Chunyan,Liu Ying,Zhang Jingtong,Tan Haiwen,Yin Pengfei,Zhang Rui,Dong Cunku,Liu Hui,Du Xiwen,Yang Jing
NiFe(oxy)hydroxide (NiFeOOH) has been widely studied as a catalyst for oxygen evolution reaction (OER), but its activity is still not satisfactory. Although metal doping has been employed as a promising strategy for addressing this issue, the instability and leaching of the high-valence dopant metals remain considerable challenges. Herein, an array of Cr-doped NiFeOOH nanosheets was in situ synthesized on nickel foam via a one-step hydrothermal method. The doping of NiFeOOH with Cr was found to induce partial electron transfer from Ni and Fe to Cr atoms, thereby modulating the electronic structure of the catalyst and enhancing its intrinsic activity. Electrochemical and in situ Raman spectroscopy analyses showed that Fe active sites with lower charge density enhance the adsorption of *OH and reduce the formation energy barrier of the *OOH intermediate during OER, thereby accelerating the OER. Moreover, Fe was found to promote the transfer of additional electrons to Cr, leading to electron accumulation at Cr sites. This electron accumulation effectively prevents Cr from excessive oxidation and leaching under anode potentials, thereby maintaining the structural stability of the catalyst. The optimized Cr-doped NiFeOOH self-supported electrode exhibited a current density of 50 mA/cm 2 with an overpotential of only 239 mV and remained stable for 100 h at 600 mA/cm 2 in 1 mol/L KOH.
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CATALYSIS LETTERS
·
2025
·Vol. 155
·期 8
·1-11
·
IF 2.4
Zhang Lan,Zou Zhipeng,Li Na,Tan Cong,Hao Baofei,Ma Huizhong
The residual antibiotics in the water environment pose a great threat to the ecological environment and human life safety. However, an effective and environmentally friendly method for degrading antibiotics is still lacking. Herein, a novel Bi 2 WO 6 catalyst with optimized morphology and abundant crystal defects was designed to remove tetracycline. The experimental results showed that the generation of grain boundaries and dislocations were induced through the epitaxial growth method, which endowed Bi 2 WO 6 with rapid separation and transfer of photoinduced electrons. Moreover, the specific surface area and light adsorption capacity of optimized catalyst (0.5Bi-Bi) were significantly improved. As a result, 0.5Bi-Bi exhibited the excellent photocatalytic performance, which degraded 90.78% of TC within 40 min under visible light illumination. Meanwhile, the synthesized 0.5Bi–Bi catalyst has robust photocatalytic stability and sustainability. The capture experiment proved that ⋅O 2 − was the prime active substance, and a possible mechanism of photodegradation of TC was proposed. Graphical
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POLYMER BULLETIN
·
2025
·
IF 4
Alassod Abeer,Alkhateeb Weaam,Alghoraibi Ibrahim,Alsabbagh Bassel,Hasan Aus Ali,Almenweer Ibrahim,alkutani M. H. D. abdalkabir,Amer Maysaa,Hlal Alaa,Dagher Wael
In this work, nanocomposite membrane-induced nanoparticles (cobalt–iron) have been successfully fabricated. The morphology observations revealed that the average fiber diameters gradually decreased. The presence of nanoparticles (iron–cobalt) was validated by X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS) analysis, indicating the successful modification. Parameters such as PH, contact time, sorbent mass, and temperature were examined to study their effect on adsorption behavior. The hydrophobicity of the composite membrane was measured using the water contact angle (WCA) test. The results showed that the WCA of obtained samples improved, where the highest WCA was around 146.45°, proving that the fabricated nanofibers were hydrophobic. The higher wicking height for PAN@Fe2 and PAN@Co2 was revealed to be (67 mm,61 mm) and (55 mm,50 mm) for soybean and motor oils. A modified new composite was successfully applied to absorb oils with a significant sorption capacity for different common oils and organic solvents. Besides, it exhibited good reusability and repeatability. All samples obeyed the pseudo-second model with a coefficient value R 2 > 0.999. The isotherm adsorption for obtained nanocomposite samples was well-fitted with the Langmuir model with a maximum reach in the range of 51.02 g/g–149.25 g/g for n-hexane and motor oil, respectively. Furthermore, the thermodynamic study revealed that the adsorption process was spontaneous and exothermic. This work is environmentally friendly, economical, and practical. It can produce superhydrophobic nanocomposite membranes on a large-scale production, which holds great potential in oil–water adsorption applications.
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CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2025
·
IF 3
Nie Saiyu,Zhang Peng,Zhang Qian,Ding Caifeng
The contamination of marine environments with antibiotics, such as kanamycin, poses a significant ecological threat due to its potential harmful effects on marine organisms. Conventional methods for detecting kanamycin, including high-performance liquid chromatography (HPLC) and enzyme-linked immunosorbent assays (ELISA), face challenges in terms of cost, complexity, and long detection times. This research aims to address these limitations by developing a novel, highly sensitive biosensing platform for kanamycin detection based on a DNAzyme-driven cascade reaction coupled with nanoparticle technology. The method utilizes aptamers for specific kanamycin recognition, which activates DNAzyme-1, triggering a downstream DNA walker system. This cascade reaction amplifies fluorescence signals, significantly enhancing sensitivity. The optimized platform demonstrated a wide detection range (0.05–3 nmol/L) and a low detection limit of 0.0327 nmol/L. This system showed excellent specificity for kanamycin, even in the presence of other antibiotics, and was successfully applied to detecting kanamycin in seawater samples with high recovery rates (94%–102.9%). This DNAzyme-based biosensor offers a promising approach for rapid, cost-effective, and high-sensitivity monitoring of antibiotic contamination in aquatic environments, with potential applications in environmental monitoring and ecological protection.
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CATALYSIS LETTERS
·
2025
·Vol. 155
·期 7
·1-12
·
IF 2.4
Wang Li,Song YingJie,Guo Yi,Chen Guobing,Zhang Hua,Liang Changhai
Cyanoacetamides(CAAM) are key precursors for the synthesis of agrochemicals and bioactive organic compounds. The activity of nitrile hydrolase (NHase) on nitrile substrates was greatly improved in our previous studies by optimizing the ribosome binding site and α/β subunit expression balance. However, during the NHase-catalyzed hydration of malononitrile, this increased catalytic activity greatly facilitated the further conversion of the intermediate cyanoacetamide to the by-product malonamide, thus preventing the enrichment of cyanoacetamide. To solve this problem, we designed a mutant library of NHase by substrate channel modification based on the strategy of semi-rational design. Among them, the βM40A mutant showed 7.68% higher regioselectivity than wild-type NHase. Structural and mechanistic analyses showed that the mutant (1) remodeled the substrate binding pocket, (2) narrowed the substrate channel, and (3) increased the metal ion-carbonitrile distance by 1.1 Å. Our results suggest that the enzyme’s regioselectivity for the product can be controlled by rational design of key amino acids in the NHase substrate channel, which provides an effective strategy for enrichment of the monocyanamide product. Graphical
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TRANSITION METAL CHEMISTRY
·
2025
·
IF 1.7
Huang Na-Jing,Li Guo-Hua,Xu Long-Jun,Wang Xiao-Yi
Using a one-step co-reduction method, AgCu bimetallic nanoparticles have been successfully loaded onto few-layer boron nitride nanosheets (BNNSs), which possess high thermal conductivity. The structure and morphology of both the support and the catalyst were characterized using X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and high-resolution transmission electron microscopy (HRTEM). Furthermore, the influence of near-infrared laser irradiation on the catalytic performance of the catalyst was investigated. The study found that the Ag 1 Cu 1 /BNNSs nanocomposite exhibited significant catalytic activity in the reduction of 4-nitrophenol (4-NP). This nanocomposite had an activation energy of only 42.9 kJ/mol and maintained high catalytic activity even after six cycles. Additionally, it was found that near-infrared laser irradiation further enhanced the catalytic activity of the composite material. This enhancement was primarily attributed to the photothermal effect of Ag nanoparticles. Moreover, the BNNSs possess high thermal conductivity. They transferred the photothermal energy generated by the Ag nanoparticles to the external environment, thereby further enhancing the local thermal effect of the catalyst. This work provided a foundation for advancing near-infrared or solar photothermal-enhanced bimetallic nanocomposite catalytic systems.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·Vol. 23
·期 1
·1-18
·
IF 12.6
Zhang Yifan,Zhou Ziyi,Liu Na,Wang Jing,Guo Qingxia,You Yong,Mao Kaiping,Wang Yuanfei,Zhang Weina,Wu Tong
Excessive scarring can impede the normal skin movement, resulting in decreased body flexibility and functional abnormalities. These issues, in turn, impact patients’ daily lives and comfort. To prevent the pathological healing of infected wounds, this study developed a composite hydrogel microneedle that possesses stable mechanical properties and favorable biocompatibility. The microneedles, composed of gelatin methacryloyl hydrogels, were integrated with curcumin-loaded PLGA microparticles to promote wound healing and allow the controlled release of curcumin within a suitable timeframe, thereby minimizing the formation of hypertrophic scars. Zinc oxide nanoparticles were integrated into the base layer of the microneedle, forming a physical barrier and exhibiting significant antibacterial activity against both Staphylococcus aureus and Escherichia coli . Due to the unique structural and functional design, we demonstrated that this class of microneedles could accelerate the healing of infected wounds and downregulate the expression of α-SMA and collagen I in scar fibroblasts, thereby showing potential to inhibit scar formation. In vivo investigations further validated the effective repair of infected wounds in rat skin and demonstrated the inhibition of pathological scars using hypertrophic scar models in rabbit ears. Altogether, these materials exhibit promising potential to provide a new option for preventing pathological scarring in the treatment of infected wounds.
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