iScience
·
2024
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IF 4.6
Ziqi Wang,Tongtong Li,Dongkai Liu,Mengjing Li,Shangming Liu,Xiaochen Yu,Hanzhen Li,Hui Song,Wei Zhao,Zhaojian Liu,Xiangfeng Chen,Gang Lu,Zi-Jiang Chen,Tao Huang,Hongbin Liu
Spermiogenesis defines the final phase of male germ cell differentiation. While multiple deubiquitinating enzymes have been linked to spermiogenesis, the impacts of deubiquitination on spermiogenesis remain poorly characterized. Here, we investigated the function of UAF1 in mouse spermiogenesis. We selectively deleted Uaf1 in premeiotic germ cells using the Stra8-Cre knock-in mouse strain ( Uaf1 sKO), and found that Uaf1 is essential for spermiogenesis and male fertility. Further, UAF1 interacts and colocalizes with USP1 in the testes. Conditional knockout of Uaf1 in testes results in disturbed protein levels and localization of USP1, suggesting that UAF1 regulates spermiogenesis through the function of the deubiquitinating enzyme USP1. Using tandem mass tag-based proteomics, we identified that conditional knockout of Uaf1 in the testes results in reduced levels of proteins that are essential for spermiogenesis. Thus, we conclude that the UAF1/USP1 deubiquitinase complex is essential for normal spermiogenesis by regulating the levels of spermiogenesis-related proteins.
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CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Xueping Chen,Hao Li,Kai Yu,Yanan Huang,Jianming Pan
Asymmetric nano-badminton is capable of enhancing the biocompatibility and maturation of immune cells, which in turn enhances its biomedical applications. This approach, in turn, enhanced biomedical applications compared with asymmetric spheres. However, this oriented structure and function of such a targeted product has not been universally explored. Moreover, enhancing the hollow architecture of nano-badminton for multifunctionality applications, such as cargo loading, remains an uncharted territory in this domain. In this work, a facile and continuous synthesis strategy was reported for asymmetric hollow nano-badminton with favorable biocompatibility by hydrolysis-condensation control engineering, which is the mediating via the hydrolysis-condensation kinetics of silane coupling agents. Due to its hydrodynamic morphology and hollow structure, silica nano-badminton has superior biocompatibility, and excellent blood lead capture and removal capacity (as high as 79%), outperforming commercial activated carbon by nearly 45% and making it viable for clinical blood purification. Three independent cell-based assessments consistently showing preserved viability and negligible toxicity validate the clinical safety of silica nano-badminton. This study provides a novel reference for the fabrication of silica hollow nano-badminton for biomedical applications.
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Journal of Thoracic Disease
·
2024
·Vol. 16
·期 11
·7941
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IF 2.1
Guowen Zou,Lei Jiang,Bin Xu,Jingyuan Xu,Zhikai Zeng,Lingchun Xia,Jian Tang,Bentong Yu
The scarcity of viable donor lungs is a formidable obstacle in lung transplantation (LTx) surgery. This issue significantly hinders the availability of this life-saving procedure for patients in need. To alleviate this shortage, researchers have turned to ex vivo lung perfusion (EVLP) technology as a means to evaluate and potentially repair marginal donor lungs, thereby increasing the donor pool and improving access to transplantation. However, it is important to note that the current capabilities of EVLP technology are still somewhat limited, and further research, development, and optimization are necessary to maximize its potential. To advance this field of study, animal experiments play a crucial role in providing a controlled environment for exploring the possibilities and limitations of EVLP. Among the various animal models available, the small animal EVLP platform for rats stands out by virtue of its simplicity, convenience, and cost-effectiveness. This platform offers researchers a unique opportunity to gain valuable insights into the workings of EVLP and its potential applications in LTx. However, conducting these experiments is not without its challenges. The complexity of the procedure requires a deep understanding of the relevant considerations and operational skills. To ensure the success of these experiments, it is essential that researchers have a comprehensive grasp of the intricacies involved. Drawing upon our practical experience over the past 3 years, our Jiangxi team has systematically refined and summarized the specific details of a rat EVLP experiment in this paper. It encompasses a comprehensive overview of the experimental setup, procedural steps, and potential pitfalls that should be kept in mind. We believe that this information will serve as a valuable resource for researchers in the field, providing them with a solid foundation upon which to build their own experiments and further advance the study of EVLP in LTx.
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CHEMOSPHERE
·
2024
·
IF 8.1
Xian Zhang,Ze Liu,Hafiz Ihsan Ul haq Khan,Diederik P.L. Rousseau,Stijn Van Hulle
This study explored Mason pine-derived hydrochar (MPHC) as an effective adsorbent and persulfate (PS) activator for degrading bisphenol A (BPA). Increasing MPHC dosage from 0.25 to 2.0 g L −1 raised BPA removal from 42% to 87%. Similarly, at the same MPHC dosage range and fixed PS concentration (8 mM), BPA removal by MPHC/PS increased from 66% to 91%. Additionally, at a fixed MPHC dosage (1.0 g L −1 ), higher PS concentrations (2–32 mM) resulted in an overall BPA removal increase from 78% to 99%. The optimal pH for BPA removal by MPHC was at pH 3, while for MPHC/PS was at pH 9. BPA degradation by MPHC was optimal at pH 3, whereas MPHC/PS was at pH 3 and pH 9. Additionally, pH 7 favored BPA adsorption for both MPHC and MPHC/PS. The study also considered the influence of coexisting anions and humic acid (HA). PO 4 3− and NO 3 − influence adsorption on MPHC, but these anions' effect on MPHC/PS is limited. Furthermore, the existence of HA had minimal influence on BPA removal by MPHC/PS. The contributions of different reactive species by MPHC for BPA degradation are as follows: electron-hole (h + ) 2%, singlet oxygen ( 1 O 2 ) 7%, superoxide radicals (O 2 •– ) 13%, electron (e – ) 2%, hydroxyl radical ( • OH) 3%, whereas the remaining 48% removal was the contribution of adsorption. For MPHC/PS, adsorption accounted for 39 %, more reactive species were involved in degradation, and the donations are (h + ) 3%, sulfate radicals (SO 4 •– ) 3%, ( 1 O 2 ) 19%, (O 2 •– ) 15%, (e – ) 2%, and ( • OH) 2%. Additionally, the performance of MPHC remains stable after three operational cycles. The preparation cost of MPHC is 3.01 € kg −1 . These results highlight the potential of MPHC as an environmentally friendly material for activating PS and removing organic pollutants, suggesting its promising application in future environmental remediation efforts.
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ACS ES&T Water
·
2024
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IF 4.8
Ze Zhang,Wankui Shao,Qiaoling Xu,Shuying Shen,Huaman Sun,Jiehao Zhan,Beini Gong,Lihua Cui,Zhujian Huang
In this study, an electrochemical system combined with peroxymonosulfate (PMS) and chlorine was developed to enhance the removal efficiency of Microcystis aeruginosa. The almost complete inactivation of algal cells can be achieved in 30 min at a chlorine concentration of 30.0 μM, PMS concentration of 0.75 mM, and current density of 15 mA/cm2. Quenching experiments indicate the involvement of •OH, SO4•–, Cl•, •O2–, and 1O2 in the inactivation of M. aeruginosa, and SO4•– plays a crucial role. Besides the inherent contribution of SO4•– in fortifying the electrolysis/PMS/chlorine system, the ability of the system to control algal proliferation is also bolstered by the SO4•– indirect activation of chlorine species. The alterations in superoxide dismutase, CAT, and malondialdehyde activities substantiate the induction of apoptosis in algal cells. The production of free radicals precipitates a cascade of oxidative impairment within algal cells, which is characterized by marked transformations in cell morphology and structural integrity. Further, microcystin-LR in actual water body also could be effectively removed in the electrolysis/PMS/chlorine system. The findings of this study convincingly establish the electrolysis/PMS/chlorine system as an efficient and environmentally friendly electrochemical application in alga wastewater treatment.
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Science Advances
·
2025
·Vol. 11
·期 12
·
IF 12.5
Xiaoran Zhang,Yanhong Lyu,Jingjing Ding,Xin Wang,Bernt Johannessen,San Ping Jiang,Jianyun Zheng
l-phenylalanine (l-Phe), an essential amino acid for humans, is widely used as building blocks. Currently, l-Phe is obtained via biosynthetic methods including microbial and enzymatic processes, but their tightly complex feedback regulation and lengthy reaction steps lead to a low practical yield of l-Phe. Here, we have designed a hierarchical Si-based photocathode for l-Phe synthesis by photoelectrochemical coupling of waste CO2 and nitrophenyl ethane, achieving a high yield rate of 37.5 μg·hour−1·cm−2 and a remarkable Faradaic efficiency of 21.2% at low applied potential under 1 sun illumination. The hierarchical structure with CuO-TiO2-C mixtures dispersed in amorphous TiO2 layer/n+p-Si creates an internal built-in electric field and forms plentiful conducting channels to efficiently realize the injection of electrons into Cu and Ti sites. These Cu and Ti sites adsorb and activate the CO2 and nitrophenyl ethane, respectively, cooperatively facilitating the l-Phe synthesis. This work introduces an environmentally friendly and highly efficient approach for converting solar energy into valuable amino acid products.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2025
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IF 9
Ruzhen Zhao,Manxing Huo,Ziwen Ying,Lijuan Liu,Qifeng Wei,Xiulian Ren
Lithium and boron are important strategic resources related to national defense and people’s livelihood. We invented an acidic extraction system N221 for simultaneous extraction of lithium and boron. However, in the process of extracting Li + from acidic extraction system, the pH of the system will inevitably decrease, which will increase the acidity during stripping. Therefore, we propose to add N235 to N221 to form an ion-pair extraction system, so that Li + can enter the organic phase in the form of neutral compound LiCl. The results show that the ion pair system can effectively improve the pH of the system and increase the operating pH range during stripping, and it is not necessary to use acid for stripping. This not only avoids the corrosion of equipment by the use of acid, but also avoids the harm to the environment caused by the use of acid. And the addition of N235 effectively reduced the Mg/Li ratio. Besides, N221 extractant has good regeneration performance and can meet the later industrial practical application. This experiment overcomes the shortcomings of N221 system, and it can be foreseen that this study also has broad application prospects in recovering lithium from spent lithium-ion batteries.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
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IF 5.4
Lu Wang,Lijuan Zhang,Fangtong Liu,Shuncheng Lin,Jun Wu,Panmin Guo,Aniruddha Majumder,Jie Lu
Carbamazepine (CBZ) is a widespread polymorphic drug, with Form II being the metastable polymorph that exhibits higher solubility, making it more attractive for pharmaceutical applications. However, Form II is prone to rapidly converting into the thermodynamically stable Form III at ambient temperatures. In this study, CBZ was crystallized in an aerogel synthesized by crosslinking TEMPO-oxidized cellulose nanofibers (TOCNF) with citric acid, resulting in the formation of the metastable Form II polymorph. PXRD and DSC analyses demonstrated that the aerogel could stabilize Form II at both room and high temperatures. Further storage studies indicated that the carboxyl and hydroxyl groups within the aerogel formed hydrogen bonds with Form II, effectively inhibiting its transformation to the stable polymorph. Molecular dynamics simulations revealed the interaction between cellulose nanofibers and the crystal face of different CBZ polymorphs. The results indicated that the interaction with Form II was the strongest, followed by Form III, while Form I exhibited the weakest interaction. These findings not only provide experimental evidence supporting the stability of CBZ’s metastable polymorph but also offer insights into potential strategies for stabilizing metastable forms of other drugs. This research could pave the way for the development of more effective pharmaceutical formulations that leverage the benefits of metastable polymorphs.
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Science Advances
·
2025
·Vol. 11
·期 32
·
IF 12.5
Chao Liang,Jingyang Zhao,Pingdong Wei,Kang Wei,Hanqing Jiang
Comprehensive liver function monitoring is vital for liver damage diagnoses. However, multiplexed testing with clinical accuracy often requires paralleled liquid manipulation to avoid reagent interference and nonspecific adsorption, making it restricted to centralized facilities and specialists. Here, we report an equipment-free, quantitative, and multiplexed point-of-care testing (POCT), the energy-embodied POCT (EE-POCT). The driving energy (pressured gas) and liquid reagents are embodied in separate rigid-flexible chambers through a mechanical pressure sensing mechanism to achieve a paralleled, interference-free, and multiple-reagent operation with no need for external equipment. The EE-POCT achieves quantitative results for nine liver-related indicators from 180 microliters of whole blood within 4 minutes. Validated with 103 clinical specimens, it shows high concordance with a clinical gold standard analyzer (Pearson coefficients >0.9). The EE-POCT platform not only advances liver health diagnostics but also sets a precedent for developing versatile, accessible medical technologies that can significantly affect global health outcomes.
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Journal of Materials Research and Technology-JMR&T
·
2025
·
IF 6.6
Minghang Gong,Yihang Cai,Dongwei Wang,Lei Wu,Yong Wang,Gongwang Cao,Yuwei Liu,Quanzhong Guo,Chuan Wang,Yang Li
During marine navigation, ships inevitably encounter corrosion and biofouling from seawater, necessitating anodes with superior performance for impressed current cathodic protection of hulls. In this study, a hybrid material comprising reduced graphene oxide (RGO) and carboxylated multi-walled carbon nanotubes (CNTs), along with a TiO 2 nanotube arrays (TNTs) interlayer, was introduced into a metal oxide-coated electrode. The RGO-CNTs/RuO 2 –IrO 2 –SnO 2 /TNTs electrode was fabricated via thermal decomposition, and its surface morphology, electrocatalytic activity, practical chlorine evolution and antibacterial performance, as well as accelerated life, were systematically evaluated. The underlying mechanisms by which the hybrid material and interlayer enhance anode performance were analyzed. The results indicate that the electrode achieves a difference of 0.430 V between chlorine evolution potential (CEP) and oxygen evolution potential (OEP), with an accelerated life of 232 h. It effectively exerts a stimulatory effect on microorganisms, thereby achieving superior biofouling resistance. This enhancement is likely attributable to the synergistic effects of RGO-CNTs and TNTs, including barrier effects, labyrinth effect, mechanical reinforcement, and optimized electron transport, which collectively improve the electrocatalytic activity and stability of the anode. This study provides valuable theoretical insights for enhancing the activity and stability of coated electrodes, holding significant promise for applications in marine corrosion protection.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Linchuan Zuo,Chuanxuan Zhou,Zhiguang Guo
Anti-icing textiles were of great significance for outdoor research and exploration activities in polar environments. However, existing anti-icing textiles often fail to achieve the requirement for efficient thermal conversion under the extreme low-temperature conditions of the polar regions, which limited their practical applications. To address this challenge, silver nanoparticles (Ag NPs) and MXene nanosheets were synergistically loaded onto the polylactic acid (PLA) nonwoven fabric fiber network via redox deposition and impregnation coating techniques. Subsequently, the surface was modified with 1H,1H,2H,2H-perfluorooctyl triethoxysilane (FAS) to construct an anti-icing textile with efficient thermal conversion. The test results indicated that the modification with FAS significantly reduced the surface free energy of the textile and endowed it with superhydrophobicity (WCA = 151.5°). It can delay freezing for 90.1 min at −5 °C, and even under extremely cold condition at −20 °C, it can still delay freezing for 7.3 min. The uniform distribution of Ag@MXene endows the textile with excellent electrical conductivity ( R = 2.84 Ω) and photothermal conversion efficiency (PCE = 87.4 %). Under a constant voltage of 3 V, the temperature stabilized at 76.5 °C, and it can completely melt an ice layer with a thickness of 0.5 cm within 361 s. Under solar radiation with an intensity of 100 mW•cm −2 , the surface temperature of the textile can reach 71.2 °C, and it can rapidly melt a 10 μL ice droplet completely within 40 s. In addition, this textile also features excellent antibacterial rate, self-cleaning property, acid and alkali resistance, as well as good mechanical properties. Its degradable PLA base material, in coordination with multi-functional carriers, provided a new idea for the development of polar tents.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Jiayu Li,Yanchao Wang,Zihao Zhu,Yaoguang Chang,Changhu Xue
Umami peptides, as critical contributors to food flavor, necessitate precise methodologies to identify novel sequences and clarify their mechanisms for advancing the understanding of umami perception. This study aimed to develop an integrated pipeline for investigating umami peptides from oysters. A portfolio of 327 short peptides (2–5 amino acids) was identified from oyster hydrolysates using UPLC-Q-Orbitrap-MS/MS. Twelve potential umami peptides were further selected via virtual screening, and their interactions with the recombinant T1R1-Venus flytrap domain protein were validated using fluorescence spectroscopy. Among them, DVVHP exhibited the highest binding affinity, with a binding constant of 5061.74 M −1 , 324.26-fold stronger than monosodium glutamate. Molecular docking and isothermal titration calorimetry revealed that the binding was dominated by hydrophobic interactions, supplemented by hydrogen bonds and electrostatic forces. This pipeline provides a systematic framework for efficient umami peptide identification in aquatic products, illuminating their interaction mechanisms with umami receptor to facilitate novel flavor enhancer development.
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危险品化学品经营许可证（带存储）