INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
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IF 8.025
Haoyuan Li,Hongxiang Zhu,Zongyan Quan,Zhiping Chen,Lei Wang,Hui He
Gold nanoclusters with excellent fluorescence intensity, stability and biocompatibility have promising application prospects in the field of detection. Inspired by the growth behavior of sea squirts adhered to ropes, a novel bionic bifunctional cellulose nanocrystal (CNC)-based gold nanocluster probe was effectively constructed by directionally growing gold nanoclusters on CNCs modified with a specific DNA sequence . The constructed probe has excellent fluorescence properties and stability. The “ON or OFF” conversion mode of fluorescence was designed to detect different pathological markers in cells. In the “ON” mode, the probe readily combined with various sulfhydryl group containing markers (acetylcholinesterase, cysteine and glutathione) to produce fluorescence quenching and realize detection, the corresponding detection limits were as low as 0.015 mU/mL, 0.028 μM and 0.228 μM, respectively. Interestingly, the fluorescence of the probe could be regulated to the OFF mode via the assembly of the graphene oxide structure in accordance with Förster resonance energy transfer . The fluorescent probe with OFF mode could selectively recognize the carcinoembryonic antigen biomarker of malignant tumours via fluorescence enhancement, and its limit of detection was as low as 0.54 ng/mL. The as-developed novel strategy has positive significance for the early diagnosis of malignant tumours.
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高影响因子
PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
·
2022
·Vol. 40
·期 1
·2200159
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IF 3.467
Yiliang Xie,Baowen Dong,Xuan Zhang,Jimin Fan,Jianying Lin,Bing Zhang,Zhihuan Zhao
In this study, biosynthetic Ag@AgCl plasma is used as a photosensitizer for the first time to construct a photothermal/photodynamic/chemotherapy and pH-responsive synergistic antitumor nanomedicine delivery system CS-Ag@AgCl/C. The Ag@AgCl and AgNPs synthesized by the extracts of alpine banana, C itrus reticulata , and Citrus sinensis are characterized. The Ag@AgCl NPs synthesized with banana extract have a regular crystal shape and a granular size of 12 nm, and they are more stable than AgNPs synthesized with the other two extracts. In addition, Ag@AgCl plasma used as a photosensitizer can reduce the dependence of CS-Ag@AgCl/C on oxygen in the photodynamic process. After coating with chitosan, though the photothermal conversion efficiency of CS-Ag@AgCl/C decreases from 44.06% to 29.51%, the pH response character of it increases, and the pH response plays an important role in drug release experiments. Under an acidic environment of pH = 5.0 phosphate-buffered saline buffer, the drug release rate of the samples increases significantly. In the synergistic antitumor experiment, the cell survival rate increases from 36.83% to 78.69% after chitosan-coated Ag@AgCl/C NPs. When CS-Ag@AgCl/C-DOX+808 nm is co-cultured with HeLa cells, the cell survival rate is only 14.89%, which indicates that the constructed plasma drug delivery system has excellent photothermal/photodynamic/chemotherapy combined treatment capability.
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高影响因子
Pharmaceutics
·
2022
·Vol. 14
·期 11
·2307
·
IF 6.525
Xueyan Gou,Fenglin Ran,Jinru Yang,Yanrong Ma,Xin’an Wu
Background: Organic anion transporter 1 (OAT1) and OAT3 have an overlapping spectrum of substrates such that one can exert a compensatory effect when the other is dysfunctional. As a result, the knockout of either OAT1 or OAT3 is not reflected in a change in the excretion of organic anionic substrates. To date, only the mOAT1 and mOAT3 individual knockout mouse models have been available. Methods: In this study, we successfully generated aSlc22a6/Slc22a8double-knockout (KO) rat model using CRISPR/Cas9 technology and evaluated its biological properties. Results: The double-knockout rat model did not expressionmRNAfor rOAT1 or rOAT3 in the kidneys. Consistently, the renal excretion ofp-aminohippuric acid (PAH), the classical substrate of OAT1/OAT3, was substantially decreased in theSlc22a6/Slc22a8double-knockout rats. The relativemRNAlevel ofSlco4c1was up-regulated in KO rats. No renal pathological phenotype was evident. The renal elimination of the organic anionic drug furosemide was nearly abolished in theSlc22a6/Slc22a8knockout rats, but elimination of the organic cationic drug metformin was hardly affected. Conclusions: These results demonstrate that this rat model is a useful tool for investigating the functions of OAT1/OAT3 in metabolic diseases, drug metabolism and pharmacokinetics, and OATs-mediated drug interactions.Keywords:OAT1/OAT3;CRISPR/Cas9;gene knockout;pharmacokinetic
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高影响因子
CHEMICAL GEOLOGY
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2022
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IF 4.685
Ming Zhang,Ying Xu,Ke-Qing Xiao,Chun-Hui Gao,Shuang Wang,Di Zhu,Yichao Wu,Qiaoyun Huang,Peng Cai
Extracellular polymeric substances (EPS) exuded by microbes are found in diverse habitats, including soil. The quantification and characterization of EPS are essential prerequisites for understanding the ecological functions of extracellular products in soil. However, few studies have conducted in-depth profiling of soil EPS extraction steps and chemical characteristics. Here we examine the C and N contents, biopolymer contents, and optical properties of EPS involving three soil pre-treatment methods (moist soil, moist soil pre-incubated, and air-dried soil pre-incubated) and simultaneously track the compositional changes during the two-step extraction process (extract with CaCl 2 in Step 1 and phosphate buffer solution (PBS) with cation exchange resin (CER) in Step 2). The optical properties of soil EPS are investigated using ultraviolet-visible adsorption (UV–Vis), excitation-emission-matrix fluorescence (EEM), and fourier transform infrared (FTIR) spectroscopy coupled with multivariate analytical methods such as parallel factor analysis (PARAFAC), principal component analysis (PCA), and correlation analysis. Results show that Step 2 releases significantly more dissolved organic carbon and biopolymers (polysaccharides, proteins, and uronic acids) from soils. UV–Vis and EEM spectroscopy suggest that the fractions extracted in Step 2 exhibit higher mean aromaticity, molecular weight , and humification degree than those extracted in Step 1. When using the standard method to extract EPS from moist aggregates (Step 2), EPS has a distinct fluorescence profile, rich in tryptophan- and tyrosine-like signals. Reassuringly, the fluorescence-profiles found in pre-extracts (Step 1) are very different - except where i) aggregates have an experimental drying step added, and ii) pure water is used as a pre-extractant. Drying of aggregates is thus problematic, and the use of pure water as an alternative pre-extractant is not advisable. Soil treatments have significant effects on the qualities of the various extracts which are detected by both the FTIR-PCA and EEM-PCA analyses. EEM fluorescence profiles of EPS suggest that both tryptophan-like and tyrosine-like substances are key components of the EPS extracted from all soils regardless of pretreatment. Our findings indicate that chemical characterization of soil EPS combined with spectral-chemometric approaches can be applied to identify and characterise the extracellular responses of soil microbes and thus help reveal the ecological functions of extracellular products in soils.
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 47
·期 4
·2079-2085
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IF 3.925
Shuwen Li,Zhiguo Wang,Siyi Chen,Jialin Gu,Yanping Ma,Jiangang Jiang,Rui Zhang,Dan Zhang,Yiping Wang,He Wang
Based on the internal filtration effect between upconversion nanoparticles and square acid-FeIII(SA-FeIII), this method was developed for the rapid identification of total antioxidant capacity of different fruits without complex modification on the surface of upconversion nanoparticles. The near-infrared light excitation of rare-earth upconversion can effectively avoid food fluorescence and the photobleaching effect; thus, the components to be detected in the samples can be monitored real time without a complex extraction process. Due to the instability of the antioxidants at room temperature, the short time required for this reaction (6 min) will facilitate the study of the true antioxidant power of the samples. This method is simple and responds well to the main antioxidants of fruits and vegetables with a detection limit of 0.1 μmol L−1, such as ascorbic acid, polyphenols, and anthocyanin, and it shows good linearity in the concentration range of 0–1.0 mmol L−1.
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高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2022
·Vol. 58
·期 1
·383-396
·
IF 4.682
Luo Lan,Lv Chengkai,Xing Yukun,Ma Fen,Kong Jie,Chen Fangfang
Abstract Molecular imprinting materials can successfully improve the efficacy and safety of drugs, thus showing good potential in the field of drug delivery. However, some difficulties in imprinted materials, such as the deep embedding of imprinting sites and poor accessibility, limit their application. Herein, we have developed porous designed molecularly imprinted polymers (MIPs) based on cleavage hyperbranched polymers for thiamine hydrochloride controlled release. By introducing hyperbranched polymers with terminal double bonds and sensitive disulfide bonds, the resulting porous MIPs (PMIPs) can expose more effective recognition sites and improve the drug-controlled release behavior, where significantly improved the drug loading (101.25 mg g −1 ) compared with the porous non-imprinted polymers (PNIPs, 16.57 mg g −1 ). Moreover, under the optimized conditions of pH 1.7 and 25 °C, the whole drug release time was increased from 16 h (PNIPs) to 32 h (PMIPs). As a result, this porous structure of MIPs designed approach provides an insight to fabricating improved imprinted constituents with higher adsorption capacity and faster recognition kinetics, which not only inspires more brilliant work in the future but also paves the way for the application of MIPs in the field of drug delivery. Graphical abstract
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高影响因子
Surfaces and Interfaces
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2022
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IF 6.137
Daiwu Chen,Ruojun Peng,Xinxin Mo,Yijiao Jin,He Li
In this paper, TiO 2 @TCPP composite was prepared by TCPP (5, 10, 15, 20-tetrakis (4-carboxyphenyl) porphyrin) and titanium dioxide. It was used to construct photoelectrochemical sensor for detecting the hydrogen peroxide overexpressed in tumor microenvironment, and its mechanism of reactive oxygen species driven by light was explored, and its inhibitory effect on the growth of tumor cells was confirmed by cell apoptosis experiment. Compared with pure titanium dioxide, the composite TiO 2 @TCPP can not only absorb visible light which has almost no damage to normal cells, but also catalyze hydrogen peroxide and water to produce hydroxyl radicals which can kill tumor cells. At the same time, the photocurrent response of the composite TiO 2 @TCPP is stronger, indicating that the composite has good catalytic performance for hydrogen peroxide. The prepared photoelectrochemical sensor can quantitatively detect hydrogen peroxide. Under the optimum conditions, the linear range of hydrogen peroxide is 1.0 × 10 −5 mol/L ∼ 3.0 × 10 −3 mol/L, and the detection limit is 0.32 μmol/L (S/N = 3). The results show that the composite can be used to fabricate photoelectrochemical sensors to detect hydrogen peroxide in tumor microenvironment, and it also has the application prospect of photodynamic therapy for breast cancer.
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高影响因子
POLYMER INTERNATIONAL
·
2022
·Vol. 72
·期 5
·471-481
·
IF 3.213
Hongcai Wang,Xiuying Sun,Ting Shang,Dongze Li,Shanshan Cao,Xiuqiong Chen,Huiqiong Yan,Qiang Lin
To improve the bio-applicability of alginate hydrogels, a combination of interpenetrating network technology, oxidation of alginate, surface coverage of gelatin and endogenous crosslinking of hydroxyapatite/ d -glucono- δ -lactone complex was designed to construct homogeneous oxidized sodium alginate/polyacrylamide–gelatin (OSA/PAM-GT) composite hydrogels. The effects of mass ratio of PAM chemical crosslinked networks and OSA ionic crosslinked networks on the microscopic morphology, mechanical properties, swellability, biodegradability and biocompatibility of the composite hydrogels were comparatively studied. Experimental results showed that porous OSA/PAM-GT composite hydrogels with regular morphology can be prepared through the interpenetration of OSA ionic crosslinked network and PAM chemical crosslinked network via hydrogen bonds and Schiff bases, which not only enhanced the mechanical properties and thermal stability of the composite hydrogels, but also regulated their swelling and in vitro biodegradability. When the mass ratio of OSA to PAM was 1:2, the cell proliferation activity on OSA/PAM-GT composite hydrogels was the best, but excessive PAM reduced the living space of cells and hindered their growth due to the reduction of porosity. Considering the results obtained, interpenetrating network technology in combination with surface coverage of GT would be a useful method for alginate hydrogels to broaden their applications in the field of biomedicine. © 2022 Society of Industrial Chemistry.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
·
IF 8.025
Qian Liu,Zhenrong Tan,Dafeng Zheng,Xueqing Qiu
In order to improve the effect of anti-tumor drugs, a magnetic delivery system for targeted drug was reported. Firstly, aminated lignosulfonate (ALS) and carboxymethyl chitosan (CMCS) were used to fabricate nano Fe 3 O 4 to obtain pH-responsive magnetic Fe 3 O 4 /CMCS/ALS nanoparticles. Then the nanoparticles were loaded with doxorubicin hydrochloride (DOX), realizing the targeted delivery and controlled release of anti-tumor drugs. It was found that the amount of crosslinking agent and emulsifier were the key factors affecting the morphology and size of the magnetic nanoparticles. Under optimized conditions, the particle size was about 79.9–169.9 nm, exhibiting excellent pH responsiveness. When the drug-to-material ratio was 11:10, the DOX loading rate and the encapsulation rate of the nanoparticles was 48.68 % and 86.23 %. While the Fe 3 O 4 /CMCS/ALS-DOX particles could release 63.14 %, 56.71 %, and 14.28 % of DOX at pH 4.0, 5.3, and 7.4, respectively. The results showed that the Fe 3 O 4 /CMCS/ALS particles exhibited excellent drug loading and release behavior based on the pH responsiveness, which could be described by Langmuir adsorption model and Fick's law of diffusion respectively. MTT assay and Live/dead staining experiments also showed that the drug-loaded particles had obvious growth inhibition on cancer cells.
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高影响因子
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
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2022
·
IF 10.319
Dan Li,Danyang Wang,Ye He,Bailong Tao,Xiaoxia Liu,Yulu Yang,Lu Tan,Yuchen Zhang,Jingwei Hu,Weihu Yang,Yu Tang,Kaiyong Cai
Bacterial infection and insufficient osseointegration are critical factors affecting the long-term success of titanium-based implants. Unfortunately, the direct application of antibiotic on Ti implants easily leads to poor cytocompatibility, as well as the production of drug-resistant bacteria. So, in this work, we designed a prospective antibacterial strategy by combining photothermal and ciprofloxacin (CIP). The synergistic effect of photothermal and antibiotic may provide an effective bacteriostatic efficacy without sacrificing osteogenesis at a mild condition of moderate temperature and less antibiotic. Herein, CIP was loaded into mesoporous polydopamine (MPDA) nanoparticles ( [email protected] ), which were anchored on the surface of titanium and finally covered with sodium hyaluronate-catechol (HAc) coating. The hydrophilic HAc layer could inhibit the early adhesion of bacteria, and some bacteria could secrete bacterial hyaluronidase to accelerate the degradation of HAc. This enabled smart enzyme-triggered release of antimicrobials at the site of infection on-demand and avoided unwanted side effects on normal tissues. In addition, NIR light irradiation had a positive influence on both CIP release and MPDA nanoparticle's photothermal effect. Moreover, before anchoring [email protected] , by the construction of hydroxyapatite microstructure on Ti surface with micro-arc oxidation and alkali heat treatment, the ability of bone formation of Ti could be promoted also. Both in vitro as well as in vivo assays demonstrated that functional Ti has an excellent antibacterial effect and osteogenic ability.
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高影响因子
ENVIRONMENTAL RESEARCH
·
2022
·
IF 8.431
Jinxin Li,Dan Zhong,Yiru Chen,Kefei Li,Wencheng Ma,Shaobo Zhang,Jingna Zhang,Aoshuang Sun,Haijiao Xie
Coal pyrolysis wastewater (CPW) contained all kinds of toxic and harmful components, which would seriously threaten the natural environment and human health. However, the traditional advanced oxidation processes frequently failed to remove phenolic substances. An A 2 BO 4 -type perovskite (La 2 CuO 4 ) was successfully synthesized through sol-gel process and first applied in the treatment of CPW. More than 90% of 3, 5-dimethylphenol (DMP) was removed within 200 min at neutral conditions. Moreover, La 2 CuO 4 also displayed excellent catalytic activity and stability in the actual CPW treatment process. Results demonstrated that DMP was removed through the attack of ∙OH, ∙O 2 − and 1 O 2 in La 2 CuO 4 /H 2 O 2 system. The La 2 CuO 4 were more favorable for H 2 O 2 activation and have a lower adsorption energy than LaFeO 3 . XPS of fresh and spent La 2 CuO 4 illustrated that the decomposition of hydrogen peroxide (H 2 O 2 ) was mainly due to the redox cycle between surface copper and oxygen species. Moreover, the possible degradation pathway of DMP was deduced by identifying degradation products and analyzing density functional theory (DFT) calculations. This research provided a novel strategy for the development of perovskite-based catalytic materials on the treatment of practical CPW.
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高影响因子
TALANTA
·
2022
·
IF 6.556
Jia Liu,Rui Ma,Wei Ha,Hai-Xia Zhang,Yan-Ping Shi
The inhibition of acetylcholinesterase (AChE) from decomposing acetylcholine (ACh) is regarded as a common therapeutic strategy for Alzheimer's disease (AD). Screening AChE inhibitors is of great significance for AD treatment . In this work, AChE was physically absorbed on the synthesized MnO 2 -ZIF-67 through electrostatic interaction for the first time, and the fabricated MnO 2 -ZIF-67 immobilized AChE coupled with capillary electrophoresis (CE) was applied to screening AChE inhibitor. The established MnO 2 -ZIF-67 immobilized AChE with excellent pH, thermal and organic solvents tolerance stability was evaluated by Ellman's method. Huperzine A was used as model inhibitor to investigate the inhibition kinetics of the immobilized AChE, which possess the feasibility and applicability for its inhibitor screening. The screening mechanism of AChE inhibitor was based on the Ellman's method and measured by the changes of 5-thio-2-nitrobenzoic acid (TNB), which was produced by the reaction of thiocholine (TCh) with 5,5-dithiobis-(2-nitrobenzoic acid) (DTNB). Benefiting from the advantages of convenience and high sensitivity, the proposed platform was employed to screen AChE inhibitors from 11 compounds isolated from Inula macrophylla , and macrophyllic acid A (compound 1 ), dibutyl phthalate (compound 2 ) exhibited strong AChE inhibitory activity. Thus, this work not only provides a promising platform for monitoring AChE activity, but also holds great potential for the further exploration of active components with AChE inhibitory activity from Inula macrophylla .
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高影响因子
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