CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Feiyang Xu,Ting Cui,Haidong Shen,Hongfu Liu,Jianfei Xie,Kai Yang,Yugui Tao
Multidrug-resistant (MDR) bacterial infections remain a critical public health concern worldwide, with conventional antibacterial strategies presenting limitations such as inconsistent target localization, suboptimal therapeutic efficiency, and progressive antimicrobial resistance development. Nitric oxide (NO), a bioactive molecule, exhibits potent antibacterial effects, resulting in the emergence of NO-based gas therapy as a promising and innovative therapeutic strategy. However, these therapies present the principal challenge of effective NO delivery, owing to its short half-life and limited diffusion capacity. Hence, this study aimed to develop AI‑molybdenum disulfide (L-Arg/IR780@MoS 2 ) nanoflowers (NFs) employing L-arginine (L-Arg) as an endogenous NO precursor and MoS 2 as a dual carrier for L-Arg and IR780 iodide (IR780). Following near-infrared irradiation during photodynamic therapy, generated reactive oxygen species initiated an oxidation cascade for L-Arg-to-NO conversion. The produced NO, in combination with photothermal therapy, modulated the bacterial microenvironment. This significantly altered the expression of key genes in MDR bacteria associated with nucleotide metabolism and biofilm formation, thereby effectively disrupting biofilms and suppressing bacterial growth. Furthermore, the AI-MoS 2 NFs exhibited notable ancillary therapeutic benefits, including anti-inflammatory activity, enhanced re-epithelialization, increased collagen deposition, and angiogenesis modulation, which collectively accelerated wound healing. Altogether, the findings of this study present a synergistic therapeutic strategy designed to address the dual challenges posed by bacterial biofilms and antimicrobial resistance.
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高影响因子
FOOD CONTROL
·
2025
·
IF 6.3
Gang Yang,Xinrui Zhang,Muying Du,Kewei Chen,András Nagy,Krisztina Takács,Ferenc Hegyi,Amel Ahmed Ibrahim,Nassra Dabour,Sameh Awad,Zsolt Zalán,Jianquan Kan
Corn is highly susceptible to pathogen invasion, such as Aspergillus flavus , leading to aflatoxin contamination and posing a significant threat to food safety. Yeasts are widely applied in the food industry, and the use of yeasts for pathogen control as an alternative to physical and chemical methods has gained increasing attention. Previous studies have investigated the aflatoxin-degrading capability of Geotrichum candidum XG1 (an unconventional yeast), but its antifungal activity and application potential have not been explored. In this study, the antifungal activity of G. candidum XG1 was evaluated both in vitro and in vivo , and its impact on corn quality was investigated. The mechanisms and safety of the yeast were also further examined. The results indicated that G. candidum XG1 significantly inhibited A. flavus , reducing the fungus and aflatoxin contamination by over 90% on corn. A 75-day storage experiment assessing changes in corn color, starch, protein, fat, and fatty acid values revealed that G. candidum XG1 had minimal impact on corn quality. Compared to the control group (infected with A. flavus ), it significantly reduced the quality deterioration caused by A. flavus invasion. Gas chromatography-mass spectrometry (GC-MS) analysis identified phenylethyl alcohol as a key compound involved in the antifungal activity of the yeast. Phenylethyl alcohol inhibited A. flavus by affecting its cell morphology, energy metabolism, and defense systems. Safety evaluations, including whole-genome sequencing and animal trials, confirmed the safety of the strain. This study provides valuable insights into the application of antagonists like G. candidum XG1 for controlling pathogen in corn.
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高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Erwei Wang,Yaqin Li,Bibole Lubamba Maguy,Zaixiang Lou,Hongxin Wang,Wanqing Zhao,Xiaohua Chen
Separation and enrichment of phenolic components from Citrus medica L. var. sarcodactylis were performed to improve the antibiofilm and antibacterial activity/efficiency of the fractions for the first time. Through separation of the crude extracts, the preparation of four fractions was done by chromatography on macroporous resin. It was found that the antibiofilm and antibacterial activity of the fractions were significantly enhanced. The obtained 30% ethanol eluted fraction (EEF) and 60% EEF both significantly inhibited biofilm formation. After the second separation by polyamide resin chromatography, the activity of the fractions was also improved. Complete inhibition (100%) on biofilm formation of S. aureus was achieved at 2.0 mg/mL. The MIC of the fractions on S. aureus was decreased to 2.0 mg/mL. There has been variation of 7.3–185.6 mg/g of phenolic content in the fractions, and a strong correlation between the anti-biofilm, the antibacterial activity and the phenolic content. Chemical composition analysis showed the EEF comprised 17 phenolic compounds. Moreover, the obtained EEF exhibited much higher antibacterial activity in tofu than crude extract. Therefore, chromatography separation significantly improved the antibacterial and antibiofilm activity/efficiency of the fractions both in vitro and in tofu.
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高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2015
·
IF 2.837
Xiao-Lin Qi,Xiao Peng,Yu-Yan Huang,Lu Li,Zuo-Fu Wei,Yuan-Gang Zu,Yu-Jie Fu
In the present study, deep eutectic solvents (DESs) as new extraction solvents, are proposed for the green and efficient extraction and separation of bioactive flavonoids from Equisetum palustre L. with negative pressure cavitation-assisted extraction (NPCE) combined with macroporous resin enrichment. A series of different DES systems were investigated for the extraction of bioactive flavonoids in E. palustre. Compared with conventional extraction solvents, DES composed of choline chloride/betaine hydrochloride-ethylene glycol possessed a more effective extraction yields. In addition, the DES-NPCE extraction procedures were also optimized systematically. The optimal conditions were obtained as follows: choline chloride-betaine hydrochloride-ethylene glycol formed a DES with the mole ratio of 1:1:2, 20% water content, extraction pressure −0.07 MPa, extraction temperature 60°C, solvent to solid ratio 25:1 mL/g, and the extraction time 20 min. Furthermore, the direct enrichment of nine flavonoids in DES extraction solution were efficiently achieved using macroporous resin HPD-826. The contents of the nine target flavonoids separated by HPD-826 resin were from 0.17% to 3.37%, and the recovery yields from 57.14% to 89.25%. In conclusion, the developed DES-NPCE combined with macroporous resin enrichment can be an alternative procedure for the green and efficient extraction and separation of bioactive flavonoids from plant sample matrices.
研究领域:
天然产物化学
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植物化学
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分析化学
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药物分离与纯化
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绿色化学
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
N. Zabeti,A.R. Faraji,A.K. Kayhanizadeh,Y. Ramezan,M. Soltani
Aflatoxin M 1 (AFM 1 ) contamination is a significant factor contributing to economic losses in the dairy industry, and it can also pose human health risks. AFM 1 can be transported easily to aquatic environments from agricultural fields by rain or re-enter the food cycle through sewage. Here, a facile wet co-participation/impregnation method was applied to fabricate rice hull-spinel manganese ferrite embedded in a porous L -cysteine-trichloro- s -triazine-cellulose scaffold (CTCR-MCF) to develop a bio-adsorbent for AFM 1 . The rice hull waste was deaggregated with spinel ferrite beads and bound to the S/N/O-H conjugates-cellulose template to synthesize an amphiphilic and pH-responsive nano-biosorbent. The modified cellulosic polymer was comprehensively characterized using structural and morphological techniques. The adsorption capacity of engineered CTCR-MCF was 0.18520 mg g −1 , and it effectively removed 99.3% (and ∼ 75.2%) of AFM 1 from 50.0 μg mL −1 AFM 1 solution (and spiked cow milk) within 45.0 min under neutral pH. The Freundlich adsorption isothermal model (R 2 = 0.982) and pseudo-2st-order kinetic model (R 2 = 0.984) demonstrated that the removal occurs as a multi-layer on a non-uniform surface without chemical/physical interactions between molecules in a spontaneous/endothermic entropy-increasing process. Additionally, environmental compatibility, chemical/thermal stability, and regeneration were assessed in detail. The results showed that the durable bio-adsorbent had no significant effects on milk nutritional qualities and was successful in removing unsafe heavy metals from milk by using a less energy-intensive isolation method. These findings offer an efficient platform for “ treating toxins with biosorbent ” to mitigate their hazardous effects in environmental and food samples.
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PubMed
高影响因子
Journal of Materials Chemistry B
·
2026
·
IF 6.2
Yan Du,Miao Zhang,Hongyan Jiang,Genmeng Yang,Ruike Xu,Ziqing Lin,Zhuo Wang,Yurong Sun,Hongqiang Gao,Kuangpin Liu,Hailei Wang
Acne is a chronic skin disorder closely associated with the over-proliferation of P. acnes within hair follicles. To avoid the overuse of antibiotics and address the low efficiency of conventional transdermal drug delivery, this study reported a chitosan oligosaccharide/gallic acid-modified ε‑poly‑L‑lysine/polyvinyl alcohol microneedles (CEGP MNs) patch for acne treatment. The patch possessed sufficient needle length (600 μm) and mechanical strength (0.8 N/needle) to penetrate the stratum corneum and dissolve rapidly in the dermis, thereby releasing the active ingredients COS and EPL‑GA. Experiments demonstrated that COS and EPL‑GA effectively kill bacteria through their enhanced antibacterial action, with an antibacterial rate exceeding 80%, thus alleviating acne‑related inflammation. Furthermore, owing to the antioxidant and anti‑inflammatory properties of GA, CEGP MNs significantly reduced the expression levels of TNF‑α, IL‑6, and IL‑1β. Meanwhile, CEGP MNs up‑regulated the expression of IL‑10 and maintained VEGF at a moderate level to create favorable conditions for the acne repair phase, helping to reduce pigmentation and prevent scar formation. Consequently, in a severe acne mice model induced by P. acnes, the biocompatible CEGP MNs exhibited promising therapeutic effects. This study proposes an effective acne treatment strategy based on the transdermal delivery of natural compounds.
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PubMed
高影响因子
ANALYST
·
2023
·Vol. 148
·期 8
·1877-1886
·
IF 4.2
Jiahe Huang,Zhengjin Zhou,Chunhong Zhang,Chao Wang,Yanli Zhou,Lijia Liu,Junqing Li,Toshifumi Satoh,Yoshio Okamoto
A novel one-handed helical copoly(phenylacetylene) (CPA) bearing L-proline tripeptide pendants and a few triethoxysilyl residues was synthesized and hybridized into SiO2 porous microspheres (PMSs) during microsphere growth through the hydrolytic polycondensation of ethoxysilyl groups. Nuclear magnetic resonance and Fourier transform infrared spectroscopy results verified the successful preparation of CPA and its hybrid product using SiO2 PMSs. The chiral recognition ability of the resulting CPA with a hybridized-type chiral stationary phase (HCSP) for high-performance liquid chromatography (HPLC) was investigated, revealing its high recognition ability for selected racemates. Moreover, the HCSP showed good solvent tolerability, thus broadening the selection of suitable eluents. The separation effect of the HCSP for the racemate N,N-diphenylcyclohexane-1,2-dicarboxamide (7) improved significantly after introducing CHCl3 in the eluent, resulting in separation factors equivalent or superior to common commercially available polysaccharide-based chiral stationary phases. The proposed preparation strategy provides a new and valuable method for obtaining poly(phenylacetylene)-based HCSPs suitable for a wide range of applications and eluent conditions.
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高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2023
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IF 3.4
Jieyu Zhang,Yan Chen,Wenxiang Fan,Linnan Li,Yueming Ma,Zhengtao Wang,Rong Shi,Li Yang
The combined efficacy in lowering serum lipid levels and increasing kidney protection of Plantago asiatica L. seed (Plantago) and Coptis chinensis Franch. rhizoma (Coptis) is far better than the effects of either herb alone. This finding suggests that there must be some degree of herb-herb interactions (HHI) affect potency. Here, we chose geniposidic acid (GPA), acteoside (ACT), and plantagoamidinic acid A (PLA) as active components in Plantago, and berberine (BBR) as the active component in Coptis, and, using transporter gene-transfected Madin-Darby canine kidney (MDCK) cells in combination with specific substrates and inhibitors, investigated Plantago- Coptis HHIs. We also established a UPLC-MS/MS analytical method to determine substrate content. Results showed that PLA in Plantago was a substrate of rOCT1/2 and rMATE1, and had inhibitory effects on rOCT2 and rMATE1. We also found that ACT is a substrate of rMATE1, but GPA was not a substrate of any transporter that we investigated. When BBR was used as the substrate, the inhibition rate of 10 μM PLA was 53.6% on rOCT2 and 31.5% on rMATE1. The inhibition rates of 30 μM ACT and 30 μM GPA on rMATE1 were 47.0% and 31.0%, respectively. Thus, our findings suggest that GPA, ACT, PLA, and BBR have competitive interactions that are driven by the rOCT2 and rMATE1 transporters. These interactions affect the transport and excretion of compounds and result in efficacy changes after co-administration.
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高影响因子
MOLECULAR PHARMACEUTICS
·
2023
·
IF 4.9
Qinming Xu,Xing Li,Jingang Yang,Yan Zhang,Xiaocui Deng,Gang Li,Qingmei Yuan
Four peptide amphiphiles (PA1-4) with different degrees of polymerization (DP = 40, 15, 10, and 6) were synthesized by Fuchs-Farthing and ring-opening polymerization followed by post-polymerization modification, as fully characterized by 1H NMR, FT-IR, gel permeation chromatography, and circular dichroism (CD) spectroscopy. It was found that PAs could self-assemble to form regular spherical micelles in low-concentration (about 1 mg/mL) aqueous solution, which had different contents of secondary structures and mainly adopted random coil conformations. The water solubility of PAs increases with the increase of DP, the polypeptide chain stretches randomly in water, the β-sheets decrease, and the random coil conformations dominate. When the pH of PA solution decreases or increases, intramolecular hydrogen bonds break, and molecular chains stretch, leading to a decrease of α-helix, turn conformations, and an increase of β-sheets. Meanwhile, the particle size of micelles increases. At around 0.4 mg/mL, the hemolysis ability of PA2 is negligible at pH 7.4 and 6.5 and about 33% at pH 5.5. Cisplatin (CDDP) was linked to micelles by coordination bonds to explore their potential as drug carriers, exhibiting controlled pH and reduction in dual drug release effects. MTT assay showed that the HeLa cell viability was 78% when cultured in the 13.5 μg/mL PA2 blank micelles for 2 days, while the cell viability was 60% in the CDDP-loaded micelles. Furthermore, a high concentration of PA2 (about 100 mg/mL) could self-assemble into a fibrous hydrogel at pH 5.5, which self-healed 2 h after incision and self-degraded 71% within 14 days. The CDDP-loaded fiber hydrogel exhibited a sustained release effect similar to the CDDP-loaded micelles. The cytotoxicity of CDDP-loaded fibers at 48 h was detected to be the same as that of the same amount of CDDP, and the cell viability was 7%. Therefore, we provide a new strategy for the synthesis of amphiphilic peptides with potential applications in nano-drug carriers and cancer therapy.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Kun Wang,Ying Yu,Wei Li,Da Li,Hui Li
In this study, heparin-like polysaccharides were successfully produced by sulfation of carboxymethylcellulose sodium, then a fully biobased bilayer composed of sulfated carboxymethylcellulose sodium (SCMC) and chitosan (CS) was composited on the surface of Poly (L-lactic acid) (PLA) through layer-by-layer (LBL) assembly for the potential blood-contact application such as bioresorbable vascular scaffold. The preliminary structure and bioactivity of SCMC with different degree of sulfation were investigated, and the SCMC with best performance was selected. The surface chemical compositions, morphologies and wettability of SCMC/CS multilayer-modified PLA films were researched by X-ray photoelectron spectrometer, scanning electron microscopy and water contact angle meter. A series of anticoagulation tests of SCMC/CS multilayer-modified PLA films were performed. In term of (SCMC/CS) 15 multilayer-modified PLA film, the protein adsorption and plate adhesion decreased by 44.6 % and 71.5 %, respectively, the activated partial thromboplastin time prolonged by 11.9 times and thrombin time exceed 300 s, the contact activation and hemolysis rate significantly reduced compared with unmodified PLA film. Besides, this modified PLA films performed good cytocompatibility to L929 fibroblast cells, excellent anti-inflammatory and antibacterial abilities. In conclusion, the multifunctional SCMC/CS multilayer-modified PLA films with hemocompatibility, cytocompatibility, anti-inflammatory and antibacterial properties may have promising potential in future clinical applications.
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高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Jiabo Cheng,Mengfei Tian,Zarmina Gul,Chunyu Liang,Bin Qiao,Yuan Gao,Chunjian Zhao,Chunying Li
In this study, a functionalized surface active ionic liquid (SAIL) was designed and prepared, which exhibits pH-responsive behavior and high yield for 6 target flavonoids from the leaves of Vitex negundo L.. Firstly, a series of functionalized SAIL [N-C n , N'–CO 2 H-Im]Br (n = 10, 12, 14, 16) were prepared and used for extraction of the target components, [N-C 14 , N'–CO 2 H-Im]Br showed the highest yield. Secondly, optimized extraction conditions obtained by Plackett-Burman design (PBD) and response surface methodology (RSM) were concentration of 45.5 mM, liquid–solid ratio of 11.6 (mL/g), extraction time of 38.5 min, extraction temperature of 40 °C, and ultrasonic power of 400 W. The yield obtained by [N-C 14 , N'–CO 2 H-Im]Br of vitexin, isovitexin, hyperoside, luteolin, quercetin, apigenin is 2.04, 1.81, 1.24, 1.51, 2.47 and 1.21 mg/g, respectively, which is 1.11–1.56 folds higher than non-functionalized SAIL ([C 14 min]Br) and 1.50–5.36 folds higher than traditional solvents. More importantly, the solvent system can realize the in-situ separation of the target compound by adjusting pH. When pH is changed from 6.1 to 4.9, the system is divided into two phases, and the target compounds were separated to the upper phase with a high recovery rate. Finally, molecular simulation was used to reveal the extraction mechanism of functionalized SAIL on target compounds. The results showed that the target flavonoids were compatibilized to the palisade region of micelles, and the weak interaction between the target flavonoids and functionalized SAIL was mainly van der Waals force.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Qianni Chen,Jiaqi Fan,Lianzhu Lin,Mouming Zhao
The combination of polysaccharides is an effective way to develop prebiotics with stable performance during processing and digestion for human wellness. However, there is little information on optimal screening and complementary regulation of compound polysaccharides. This study aimed to optimally select a combination of Lycium barbarum L. polysaccharide (LBP) and Laminaria japonica polysaccharide (LJP) as a highly efficient prebiotic to regulate the gut probiotics and their metabolites. Two LBPs characterized as rhamnogalacturonan I enriched pectins and two LJPs characterized as fucoidans were obtained by enzyme-assisted acid extraction at moderate and dramatic temperatures and combined in pairs to obtain 4 groups containing 4 proportional combinations. All combinations showed better prebiotic effects than individual LJP. The combination of LBP and LJP extracted at 50 °C at a ratio of 4:1 exhibited the strongest prebiotic effect. The optimal compound polysaccharide achieved superior effect and complementary function via LBP-targeted proliferation of Bifidobacterium , Lactobacillus , and Bacteroides and production of SCFAs and non-SCFA health-associated metabolites, LJP-targeted accumulation of butyrate-producing bacteria and corresponding metabolites, as well as synergistic effect of LJP and LBP at exact proportion. Our study provided theoretical and methodological guidance for optimal screening of compound polysaccharides as new prebiotics.
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高影响因子
危险品化学品经营许可证（带存储）