FOOD CHEMISTRY
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2023
·
IF 8.8
Rui Wang,Man-Qin Zeng,Yu-Wei Wu,Yong-Xin Teng,Lang-Hong Wang,Jian Li,Fei-Yue Xu,Bo-Ru Chen,Zhong Han,Xin-An Zeng
In this study, soy protein isolate (SPI) was modified by a pulsed electric field (PEF) combined with pH shifting treatment (10 kV/cm, pH 11) to prepare SPI nanoparticles (PSPI11) for efficient loading of lutein. The results showed that when the mass ratio of SPI to lutein was 25:1, the encapsulation efficiency of lutein in PSPI11 increased from 54% to 77%, and the loading capacity increased by 41% compared to the original SPI. The formed SPI-lutein composite nanoparticles (PSPI11-LUTNPs) had smaller, more homogeneous sizes and larger negative charges than SPI7-LUTNPs. The combined treatment favored the unfolding of the SPI structure and could expose its interior hydrophobic groups to bind with lutein. Nanocomplexation with SPIs significantly improved the solubility and stability of lutein, with PSPI11 showing the greatest improvement. As a result, PEF combined with pH shifting pretreatment is an effective method for developing SPI nanoparticles loaded and protected with lutein.
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Advanced Healthcare Materials
·
2023
·
IF 10
Ni Ding,Gangjian Luo,Huiting Li,Chengyuan Xing,Yuanji Gao,Wenjie Xi,Weijie Wu,Dan Wang,Lei Zheng,Yang Kang,Xinjin Chi
The mortality rate of sepsis remains high despite improvements in the diagnosis and treatment of sepsis using symptomatic and supportive therapies, such as anti-infection therapy and fluid resuscitation. Nucleic acid-based drugs have therapeutic potential, although their poor stability and low delivery efficiency have hindered their widespread use. Herein, it is confirmed that miR-223 can polarize proinflammation M1 macrophages to anti-inflammation M2 macrophages. A pH-sensitive nano-drug delivery system comprising β-cyclodextrin-poly(2-(diisopropylamino)ethyl methacrylate)/distearoyl phosphoethanolamine-polyethylene glycol (β-CD-PDPA/DSPE-PEG) is synthesized and developed to target M1 macrophages and miR-223 is encapsulated into nanoparticles (NPs) for sepsis treatment. NPs/miR-223 demonstrated in vitro pH responsiveness with favorable biosafety, stability, and high delivery efficiency. In vivo studies demonstrate that NPs/miR-223 are preferentially accumulated and retained in the inflammation site, thereby reducing inflammation and improving the survival rate of mice with sepsis while exhibiting ideal biosafety. Mechanically, NPs/miR-223 regulates macrophage polarization by targeting Pknox1 and inhibiting the activation of the NF-κB signaling pathway, thereby achieving an anti-inflammatory effect. Collectively, it is demonstrated that the miRNA delivery vector described here provides a new approach for sepsis treatment and accelerates the advancement of nucleic acid drug therapy.
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Journal of Environmental Chemical Engineering
·
2023
·
IF 7.7
Yi Liu,Dengjie Zhong,Yunlan Xu,Haixing Chang,Lin Dong,Zhuofan Han,Jun Li,Nianbing Zhong
The limited metal sites of monometallic MOFs and the high influence of pH limit their adsorption capacity. To solve these problems, La/Al bimetallic MOFs/chitosan composite was prepared by hydrothermal synthesis and impregnation methods and then characterized by XRD, FTIR, SEM, BET and XPS techniques. The doping of La has no obvious effect on the structure of Al-MOFs, but chitosan has greatly changed the structure of La/Al bimetallic MOFs. The phosphate adsorption of the composite was affected by La/Al molar ratio, chitosan/bimetallic MOFs mass ratio, temperature, phosphate initial concentration, adsorbent dosage, pH, and coexisting ions. The optimum molar ratio of La/Al and mass ratio of chitosan/bimetallic MOFs are 0.5:1 and 5.0%, respectively. The addition of La and chitosan can provide additional adsorption active sites, inhibit metal leaching, and enhance the phosphate adsorption performance, stability, and acid-alkali resistance of the composite. Its maximum adsorption capacity was 264.48 mg·g −1 . Its phosphate removal efficiency is above 90% at pH= 3.0–9.0. The actual phosphorus-containing wastewater with 7.064 mg·L −1 PO 4 3- and initial pH 7.4 can be treated to meet Chinese first-class effluent quality standard. La, Al, amino groups, and hydroxyl groups play an important role in the adsorption process of phosphate. When pH < pH pzc (4.99), phosphate is adsorbed by electrostatic gravitation between it and protonated positively charged amino and hydroxyl groups on La and Al, while when pH > pH pzc , phosphate is removed by ligand exchange between it and hydroxyl groups on La and Al. A stable, low-cost, and efficient bimetallic MOFs-based phosphate adsorbent was designed and prepared in this paper, and the results can provide reference for researchers in environmental chemical engineering .
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2021
·
IF 8.128
Qi Zhou,Xuelin Dong,Binbin Zhang,Xinwei Zhang,Kaide Ou,Qin Wang,Yonggui Liao,Yajiang Yang,Hong Wang
In this work, a pH-tunable multicolor luminescent lanthanide-based hydrogel (CS/DEX/CP) was prepared based on lanthanide coordination polymer (CP), dextran aldehyde (DEX) and chitosan (CS). The CP was obtained by the self-assembly of guanosine acid (GMP), ciprofloxacin (CIP), Eu 3+ , and Tb 3+ . As-prepared CS/DEX/CP hydrogel could emit blue, green, and red luminescence of CIP, Tb 3+ , and Eu 3+ , respectively. It was also found that the luminescence of CS/DEX/CP hydrogel exhibited visual color change in the pH range of 5.5 to 8. Such pH-sensitive hydrogel was multicolor-responsive to protons produced by bacterial growth, therefore, it could provide early warning of bacterial infection by naked-eye. In addition, the increased acidity resulted in not only the degradation of acid-labile Schiff base linkages between DEX and CS, but also the fracture of coordination between CIP and lanthanide ions. As a result, the released CIP and CS showed significantly antibacterial activity against both S. aureus and E. coli .
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2021
·
IF 7.312
Yingxin Zhao,Cailian Zhao,Ying Yang,Zhuoran Li,Xiaojie Qiu,Junzhi Gao,Min Ji
As a widely used antibiotic, sulfamethoxazole (SMX) has attracted increasing research attention. However, acidic condition was normally required for efficient SMX removal, and the energy variation of adsorption system is not fully elucidated. In this study, based on a typical mineral adsorbent of volcanic rock (VR), anion exchange was introduced by Polypyrrole (PPy) modification to overcome the electrostatic repulsion in alkaline environment, and site energy distribution (SED) theory was applied to explore adsorption behavior and mechanism on SMX from an energy perspective. The batch adsorption experiments and a series of complementary characterization were combined, confirming that pore filling and hydrogen bonding are the dominant adsorption mechanisms for SMX removal by VR. While the adsorption distinction between PPy supported VR (PPy-VR) and VR could be explained by electrostatic attraction, π-π electron donor-acceptor interaction (π-π EDA) and ion exchange, which further explained the wide pH application potential of modified sorbent. Moreover, Dubinin-Ashtakhov (DA) model was used to calculate the energy distribution, and PPy-VR exhibited a stronger adsorption affinity ( E m = 8.55–11.57KJ/mol) and energy heterogeneity ( δ e *=2.15–2.57) for SMX. The high-energy adsorption centers increased significantly (92.63%) after modification, which mainly attributed to π-π EDA and ion exchange introduced by PPy loading. The surface modification method provides a feasible idea for the improvement of application environment of adsorbents, and the analysis of SED is expected to promote the understanding of the connection between site energy and adsorption mechanism.
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MICROCHIMICA ACTA
·
2021
·Vol. 189
·期 1
·1-9
·
IF 5.833
Liu Shuqi,Liu Huiling,Chen Qinqin,Hou Juan,Yang Guang
For the first time, boronic acid–modified polymer dots (B-PDs) were fabricated by a “synthesis-modification integration” route using polyethylenimine (PEI) and phenylboronic acid as precursors. Under optimized preparation conditions, the B-PDs exhibited an average size of 2.2 nm, good water solubility, and high fluorescence quantum yield of 8.69%. The B-PDs showed reversible fluorescence response in acid solutions (blue emissions) and alkaline solutions (green emissions). The fluorescence emissions of B-PDs demonstrated an obvious red shift with varying the pH value from 1 - 13. Moreover, glucose could assemble on the surface of B-PDs due to the reversible reaction between boronic acid and cis -diols, which resulted in a blue shift of emission wavelength and an obvious increase of FL intensity at λ ex = 380 nm based on the aggregation-induced enhancement effect. The glucose sensing method was thus developed in the range 0.0001 - 1.0 mol L −1 . Applications to real human blood and glucose injection samples demonstrated satisfactory results. The B-PDs based on the analytical method display good selectivity, wide detection range, and simplicity in preparation and detection, implying promising applications as a practical platform for biosensing. Graphical abstract
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SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Ying Zhang,Qian Liu,Mengting Liu,Xueni Zhang,Xiaoya Li,Lihui Dai,Meng Meng,Dingbin Liu,Yongmei Yin,Rimo Xi
The microenvironment of lysosomes plays an important role in various functions within cells. Abnormal pH changes in lysosomes account for multiple diseases including tumors. Acid-responsive nanomaterials with reconfigurable structures and properties have garnered significant interests. Owing to the potential in biosensing, controlled release and nanomachinery, DNA based pH-responsiveness has been widely pursued. Herein, we designed an AuNPs-i-motifs based SERS probe to monitor the pH dynamics of lysosomal maturation in different cells. Taking advantages of biocompatibility and structural flexibility of DNA, unique optical and plasmonic properties of gold nanoparticles , excellent stability and enhanced sensitivity of background-free Raman reporter, the designed SERS probe could indicate the pH variation dynamically in lysosomes. What is more valuable is that 4-mercaptobenzonitrile is not only used as a Raman reporter to provide sensitive Raman signal for pH sensing, but also used as a sealing agent allowing for controllable aggregation, to improve the stability and accuracy of the sensor. With this versatile design, the sensor was prepared simply and feasibly and a tedious trial was avoided. It provides a feasible tool for understanding the relationship of lysosomal pH with fundamental cellular functions and mechanism of diseases.
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Journal of Chemistry
·
2021
·
IF 2.506
Xiaohui Song,Shuaiqi Sun,Lingzong Meng,Rongjian Ying,Yafei Guo,Tianlong Deng
The densities and pH values in the system NaBO2–Na2SO4–H2O at 298.15 K and 323.15 K were investigated. Combining the equilibrium constants for different boron species, the distributions of six boron species in the mixed solution were calculated with total boron concentration and pH values. The molar fractions of the six boron species are mainly affected by the total boron concentration and temperature, but rarely affected by the concentration of SO42–. The dominant boron species in the mixed solution at the two temperatures is B(OH)4‒. The mole fraction of B(OH)3, B5O6(OH)4‒, and B3O3(OH)4‒ can be neglected. The polyborate ions are easier to form as the temperature increases. The results of distribution for boron species in this study and those with the Pitzer model can both be used to describe the distribution of boron species in the mixed solution.
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POLYMER BULLETIN
·
2017
·Vol. 74
·期 11
·4525-4536
·
IF 1.43
Huang Zengfang,Zhang Xiqi,Zhang Xiaoyong,Wang Shiqi,Yang Bin,Wang Ke,Yuan Jinying,Tao Lei,Wei Yen
Abstract In this paper, a fluorescein methacryloyl monomer (named Flu-MA) was firstly fabricated by the esterification reaction of fluorescein and methacryloyl chloride; subsequently, a novel amphiphilic fluorescent copolymer (Flu-PEG) with smart pH sensitivity was successfully prepared by RAFT copolymerizing of Flu-MA and poly(ethylene glycol) monomethacrylate (PEGMA). The result of 1 H NMR and FTIR implied that both Flu-MA and PEGMA monomers were successfully incorporated into Flu-PEG copolymers, the molecular weight ( M
n ) of which was about 39,800 with polydispersity index (PDI) as about 1.30. In aqueous solution, Flu-PEG copolymers could self-assemble into fluorescent organic nanoparticles (FONs) with about 200 nm diameters due to high water dispersibility and good fluorescence, respectively, endowed by PEGMA and Flu-MA, the fluorescence intensity of which was high sensitive to pH value of solution. More importantly, due to their high water dispersibility, excellent biocompatibility and good fluorescence, the as-prepared Flu-PEG FONs were high attractive for the application in cell imaging. Graphical abstract A novel amphiphilic fluorescent copolymer (Flu-PEG) with smart pH-sensitivity was successfully fabricated by RAFT copolymerizing of Flu-MA and PEGMA, which showed high water dispersibility, uniform size and excellent biocompatibility, making them be highly potential for bioimaging application.
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MOLECULAR PHARMACEUTICS
·
2019
·
IF 4.396
Jiaoyang Tao,Weidong Fei,Hongxia Tang,Chaoqun Li,Chaofeng Mu,Hongyue Zheng,Fanzhu Li,Zhihong Zhu
The poor capability of drugs to permeate through the blood–brain barrier (BBB) and further release inside glioma greatly limits the curative effects of glioma chemotherapies. In this study, we prepared angiopep-2-conjugated liposome-silica hybrid nanovehicles for targeted delivery and increased the permeation of arsenic trioxide (ATO) in glioma. Polyacrylic acid (PAA) was grafted on mesoporous silica nanoparticles (MSN) for pH-sensitive release and supporting the lipid membrane. The prepared “core–shell” nanovehicles (ANG-LP-PAA-MSN) were characterized with uniform size, high drug loading efficiency (8.19 ± 0.51%), and superior pH-sensitive release feature. From the experiments, the enhanced targeted delivery of ATO by ANG-LP-PAA-MSN (ANG-LP-PAA-MSN@ATO) was evidenced by the improvement of transport, enhanced cellular uptake, and apoptosis in vitro. In addition, the pharmacokinetic study was creatively carried out through the blood–glioma synchronous microdialysis and revealed that the half-life (t1/2) of blood and glioma tissue in the ANG-LP-PAA-MSN@ATO treatment group was extended by 1.65 and 2.34 times compared with the ATO solution group (ATO-Sol). The targeting efficiency of ANG-LP-PAA-MSN@ATO (24.96%) was dramatically stronger than that of the ATO-Sol (5.94%). Importantly, ANG-LP-PAA-MSN@ATO had a higher accumulation (4.6 ± 2.6% ID per g) in tumor tissues and showed a better therapeutic efficacy in intracranial C6 glioma bearing rats. Taken together, the blood–glioma synchronous microdialysis was successful used for the pharmacokinetic study and real-time monitoring of drug concentrations in blood and glioma; ANG-LP-PAA-MSN could be a promising targeted drug delivery system for glioma therapy.
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CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Ting-Ting Li,Li Sun,Ying Zhang,Bing-Chiuan Shiu,Hai-Tao Ren,Hao-Kai Peng,Jia-Horng Lin,Ching-Wen Lou
Bacterial infection, management of wound exudate and monitoring of healing status are the most prominent problems in wound treatment, however, according to research findings, the large multifunctionality of dressings studied so far is relatively homogeneous, and making wound dressings with simultaneous antibacterial and antioxidant properties, unidirectional fluid delivery and monitoring of wound healing is still a challenge in current research. Here, based on electrostatic spinning and electrostatic spraying techniques, a multifunctional Janus composite membrane was prepared by combining a hydrophilic layer polyacrylonitrile@poly(dopamine) (PAN@PDA), a hydrophobic layer polycaprolactone@ curcumin/silk fibroin (PCL@CUR/SF), and a drug-carrying layer aminated mesoporous silica-chitosan/curcumin (MSN-NH 2 -CS/CUR) to exploit the difference in wettability between the layers, excess wound exudate was drained. Interestingly, the cumulative in vitro release rate of the composite membrane was 7.67% at 551 h. The antioxidant activity was 79.01% at 120 min. The antibacterial properties of the composite membrane against Escherichia coli and Staphylococcus aureus were about 98% and 99%, respectively, indicating the excellent drug retardation and antibacterial and antioxidant properties of the composite membrane. The color of the composite membrane slowly changed from yellow to orange-red when exposed to pH 6, 7.2, 8 and 9 buffers, indicating that the composite membrane can be used as a pH indicator in the wound healing process. MTT and cell adhesion experiments showed that the composite membrane has good cytocompatibility and is conducive to cell adhesion and proliferation. This multifunctional Janus composite membrane will be useful for wound management and treatment.
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ACS Applied Materials & Interfaces
·
2026
·
IF 7.8
Huang Zhu,Waner Li,Qingjie Li,Jianlan Li,Mengqi Zhang,Ying Liu,Qingqing Pan,Chi Liu,Rong Liu
The treatment of diabetic wounds is hindered by multiple factors, including Staphylococcus aureus (S. aureus) biofilms (the predominant pathogen in diabetic wound infections), dysregulated immune responses, and insufficient angiogenesis. The complex interactions among these local pathological factors have been insufficiently addressed in the development of existing therapeutic approaches. This study reports a hybrid biocatalytic platform consisting of abundant iridium (Ir) clusters supported on ceria (Ir-CeO2). This architectural configuration enables pH-responsive reactive oxygen species (ROS) catalysis, allowing the biocatalyst to exert concurrent antimicrobial and anti-inflammatory effects, which collectively promote healing of diabetic wounds. Ir-CeO2 displays dual pH-dependent ROS modulation: ROS generation under acidic conditions and ROS scavenging under neutral pH. In S. aureus-infected environments, the biocatalyst exerts potent antibacterial effects via catalytic ROS production. Following pathogen clearance, Ir-CeO2 continues to play a bifunctional role by alleviating oxidative stress in inflamed wounds, promoting the polarization of macrophages toward the M2 phenotype-a critical event for accelerating wound healing. Collectively, these results highlight the multifaceted therapeutic efficacy of Ir-CeO2, which seamlessly integrates potent antibacterial activity against S. aureus infections with anti-inflammatory and pro-angiogenic functions. This synergistic profile establishes Ir-CeO2 as a highly promising agent for the holistic management of complicated diabetic ulcers, thereby addressing key shortcomings of existing treatment modalities.
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