JOURNAL OF FLUORESCENCE
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2013
·Vol. 24
·期 2
·533-539
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IF 1.789
Liu Chengxuan,Liang Gaiting,Liu Zhen,Zu Lily
Time-resolved fluorescence anisotropy spectroscopy was applied to study the interaction between a peptide truncated from the binding site of tumor suppressor p53 protein and the DNAs covalently labeled with 6-carboxyfluorescein (FAM) dye. Fluorescence intensity quenching and changes of anisotropy decay lifetime were monitored when FAM labeled DNA formed complex with the peptide. The results demonstrated that the sequence of DNA could not define the binding specificity between the peptide and DNA. But the anisotropy decay of FAM can be used to examine the binding affinity of the peptide to DNA. The fluorescent dynamics of FAM can also be used to represent the rigidity of the complex formed between the peptide and DNA.
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Food Analytical Methods
·
2021
·Vol. 14
·期 5
·957-967
·
IF 3.366
Sha Junyi,Lin Hong,Timira Vaileth,Sui Jianxin
To detect enrofloxacin (ENR) and ciprofloxacin (CIP) residues in fishery foods, a computer simulation was used to predict and analyze the binding sites existing aptamer specific to ENR and CIP. A new aptamer (SEQ.3) that can recognize ENR and CIP was screened and evaluated, and the dissociation constants (K d ) of SEQ.3 from ENR and CIP were determined to be 210.955 nM and 887.49 nM which are higher than other aptamers SEQ.4 and SEQ.5 by microscale thermophoresis (MST). Colorimetric detection method based on gold nanoparticles (AuNPs) was developed through optimization of temperature, incubation time, aptamer concentration, and other conditions. The results showed that the limit of defection (LOD) of the new aptamer to ENR and CIP were 1.89 nM and 6.84 nM, respectively, which were similar to that of the original aptamer. The recovery rate of the colorimetric method was between 87.8~102.18%. All the results implied that the developed colorimetric aptamer sensor has broad application prospects in food safety detection and environmental monitoring.
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ENVIRONMENTAL GEOCHEMISTRY AND HEALTH
·
2017
·Vol. 40
·期 2
·763-776
·
IF 2.616
Wang Lanjun,Wang Jinhua,Zhu Lusheng,Wang Jun
The production of commercial livestock and poultry often involves using with antibiotics and feed additives, such as oxytetracycline (OTC) and copper (Cu). These are often excreted into the soil by animal feces; hence, combined pollutants may contaminate the soil. To evaluate single and combined toxic effects of OTC and Cu on the soil ecology, changes in quantities of bacteria, fungi, and actinomycetes in the soil were studied over a 28-d incubation period by a plate count method, microbes numbers counted on days 7, 14, 21, and 28. Abundances of ammonia monooxygenase ( amoA ) gene expression by ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) in soil samples also were tested by real-time polymerase chain reactions (RT-PCRs) on day 21. The results revealed that the numbers of bacteria, fungi and actinomycetes and amoA genes copies of AOA and AOB were reduced seriously by exposure to Cu (1.60 mmol/kg). Similarly, the combined pollution treatments (mole ratios of OTC: Cu was 1:2, 1:8, and 1:32) also had inhibitory effect on bacteria, fungi, and actinomycetes numbers and amoA gene copies of AOA and AOB; the inhibitory rate was on obvious growth trend with the increasing mole ratios. Effects from single OTC pollution were found on bacteria (days 7 and 14), fungi (days 7, 14, 21, and 28), and AOA- amoA gene copies (day 21), with promotion at a low concentration (0.05 mmol/kg) and suppression at higher concentrations (0.2 and 0.8 mmol/kg). Also, numbers of bacteria, fungi, and actinomycetes decreased with longer culture times. Combining OTC and Cu led to a higher inhibition of soil microbes than when either chemical was used alone. However, there was no significant relationship between single and combined toxic chemicals because of their complicated interactions, either antagonistic or synergistic. The results also indicated the sensitivity of bacteria, fungi, actinomycetes on toxic chemicals existed difference and that the AOA were more tolerant than the AOB to these chemicals.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2016
·Vol. 409
·期 7
·1797-1803
·
IF 3.125
He Meng-Qi,Wang Kun,Wang Jin,Yu Yong-Liang,He Rong-Huan
To specifically and sensitively identify bisphenol A (BPA) with a simple and rapid method is very important for food safety. Using an anti-BPA aptamer and Mo 2 C nanotubes, we developed a label-free and low-background signal biosensor for BPA detection. The anti-BPA aptamer drastically increased the fluorescence signal of N -methylmesoporphyrin IX under an assistance of Help-DNA. Additionally, BPA can interact with the anti-BPA aptamer and switch its conformation to prevent the formation of a G-quadruplex, resulting in fluorescence quenching. Simultaneously, Mo 2 C nanotubes can reduce the background signals due to the adsorption of Help-DNA on their surface. This method shows a linear range of 2–20 nM with a detection limit of 2 nM for detecting BPA. This label-free BPA aptasensor with low background signal is inexpensive, easy to use, and can be applied to determine BPA in real water samples.
A low-background and label-free biosensor was designed based on Mo 2 C nanotubes and aptamer for BPA detection.
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Nano Research
·
2020
·Vol. 14
·期 1
·222-231
·
IF 8.183
Zhu Huimin,Cao Guodong,Fu Yike,Fang Chao,Chu Qiang,Li Xiang,Wu Yulian,Han Gaorong
Multidrug resistance (MDR) restricts chemotherapy efficacy due to P-glycoprotein (P-gp) mediated drug efflux, whereas current approaches to suppressing P-gp expression suffer from intrinsic challenges, such as low transfection, high toxicity and poor specificity. Here, hollow ferric-tannic acid complex nanocapsules (HFe-TA), which can be effectively degraded by the reaction with adenosine triphosphate (ATP), are synthesized for the delivery of glucose oxidase (GOx) and doxorubicin (DOX) for tumor treatment. The findings indicate that the intracellular ATP is significantly decreased due to the combined effect of HFe-TA degradation and GOx-mediated glucose consumption. Along with this ATP down-regulation, P-gp expression of tumor cells is suppressed remarkably, which in turn promotes the intracellular accumulation and anticancer efficacy of DOX. In addition, the production of OH by Fe ions released from HFe-TA is promoted by the by-products of the oxidation of glucose process by GOx. In consequence, HFe-TA nanocapsules loaded with DOX and GOx enable significant inhibition effect to tumors both in vitro and in vivo due to the synergistic effect of cascade reactions. This study has therefore provided an alternative therapeutic platform for effective tumor inhibition with the potential in overcoming intrinsic MDR.
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JOURNAL OF MATERIALS SCIENCE
·
2025
·
IF 3.9
Hao Siyu,Zhu Qingsong,Peng Xingna,Fan Jiayi,He Zimeng,Li Wei
Given the rapid advancements in digital and communication technology, electromagnetic wave pollution has emerged as a pressing concern for society. In addition, wave-absorbing materials are typically utilized in complex and dynamic environments, where they are susceptible to corrosion, which can result in a reduction in their effectiveness. Herein we synthesized a polypyrrole functionalized Ti 3 C 2 MXene (PPy-TM) hybrid with significant electromagnetic wave absorption and superior corrosion resistance by in situ polymerization method, which due to the in situ polymerization process of PPy on TM's surface causes the smoothness of TM's surface to turn coarse. The composite exhibited an optimal reflection loss of − 35.5 dB at 3.60 GHz for a 7.50 mm thickness. Additionally, the value of |Z| 0.01 Hz for PPy-TM 0.5% (5.1218 × 10 4 Ω cm 2 ) was an order of magnitude higher than the WEP (7.9778 × 10 3 Ω cm 2 ) coating after 30 days of exposure to 3.5 wt% NaCl electrolyte. This study establishes a benchmark for designing next-generation materials that integrate robust microwave absorption with long-term corrosion protection.
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Nature Communications
·
2025
·Vol. 16
·期 1
·11068
·
IF 15.7
Meng Shengnan,Wang Hongxing,Zhao Qian,Lü Xiaozhou,Yu Jianyong,Si Yang
Transpiration cooling is a thermal management technique that utilizes the phase change of liquid coolant to effectively dissipate heat. Porous ceramic media play a crucial role in this cooling process by facilitating liquid transport and heat exchange; however, their intermittent capillary action and inter-grain phonon scattering significantly hinder rapid cooling. Here, we propose a strategy to create AlN-based nanofiber aerogel as a transpiration thermo-cooler, featuring vertically aligned channels and monocrystalline nanofibers by combining nanoengineering and multiscale structural assembly techniques. Benefiting from the unconstrained capillarity of aerogel channels, our thermo-coolers exhibit a fast liquid transport rate of up to 8.33 ± 0.026 mm s −1 , surpassing that of state-of-the-art porous media by one to two orders of magnitude. In addition, the enhanced phonon conduction properties of single-crystal AlN nanofibers enables thermo-coolers to achieve a fast cooling rate of 156.8 °C s −1 , outperforming advanced cooling materials by a factor of five and making them ideal for various thermal management applications.
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Nature Communications
·
2025
·
IF 15.7
Guo Yuan,Yun Yuzhe,Xiang Wenzhi,Xu Guorui,Sun Luye,Xu Wei
On-surface synthesis recently enabled access to generate a series of cyclo[ n ]carbons (C n , up to C 26 ) via delicately designed precursors. However, for larger cyclocarbons, synthesis of the corresponding precursors is being challenged. Here, we report another strategy for synthesizing larger cyclocarbons via the coupling and ring-opening reactions from smaller ones. We chose C 10 as the precursor, and induce the coupling and ring-opening reactions via tip manipulation to successfully generate C 20 and C 30 . Bond-resolved atomic force microscopy imaging unambiguously reveals the polyynic structure of C 30 , aligning with theoretical prediction. This approach establishes a new pathway to access larger cyclocarbons, and potentially enables controlled polymerization of cyclocarbons.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2025
·Vol. 36
·期 35
·2235
·
IF 2.8
Murtaza Muhammad,Khan Salman Ali,Khan Hayat,Hossain S. K. Safdar
Facile, fast and high yield synthesis of silver nanowires (Ag NWs) have always been difficult due to lack of optimized control over affecting parameters. Solution-based polyol synthetic routes have enabled Ag NWs with unique and novel properties to provide a base for a wide range of applications. Ag NWs are indispensable for flexible electronics but achieving fast high yield synthesis with single crystalline and high purity remains a challenge. Here, we report a novel, scalable solution-phase method that produces Ag NWs with \(>\) 90% yield, single-crystalline structure, and ultrahigh purity (no detectable oxides or impurities) with fast synthetic process. By optimizing a dual-reductant system (ascorbic acid/citric acid) ratio in a mixed solvent (ethylene glycol/water) with precise control of silver nitrate (AgNO₃) concentration (0.03 M) and PVP (0.09 M, MW: 360 k), we achieve high yield uniform NWs with mean diameter (μ) = 64.4 ± 16.2 nm (nm) with single crystalline nature. Finally, the potential applications of as-synthesized Ag NWs are demonstrated by successfully preparing Ag-based conductive ink. The conductive material exhibits resistivity of 11.7 µΩ cm equals 7.4 times the resistivity of bulk Ag. Flexibility and stability of the prepared Ag conductive material were determined by tensile-bending testing system and placing the Ag conductive material in air, respectively. It indicates that the prepared Ag conductive material has the potential to be used in flexible electronics and wearable equipment.
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JOURNAL OF POLYMER RESEARCH
·
2025
·Vol. 32
·期 12
·467
·
IF 2.8
Li Xiaoyun,Wang Zhen,Wang Junwei,Zhang Yingan,Kang Maoqing,Li Qifeng,Zhao Yuhua,Li Huanting
Bifunctional reactive cyclic carbonate compounds were synthesized and introduced into the epoxy system acting as diluting plasticizer and toughening agent to improve their plasticity and flexibility. Effects of content and structure of cyclic carbonates on the processibility, mechanical performances and thermal properties of the materials were investigated. An impressive improvement in viscosity reduction and mechanical performances is observed by loading of cyclic carbonate. The modified epoxy resin composites exhibit a significant enhancement in mechanical properties at a 30 wt% loading of cyclic carbonate with the tensile strength 170.6% higher than the original resin and 23.9% improvement in the flexural strength. The enhancement in material performance can be attributed to a combination of factors: the presence of soft segments, hydrogen bonding interactions, decrease in crosslinking density, and the plasticizing effect of unreacted components. Moreover, the thermal properties like degradation temperature, T g temperature and thermo-mechanical properties of epoxy resin composites were also affected by the introduction of cyclic carbonate.
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Nature Communications
·
2025
·
IF 15.7
Liu Jiaqi,Liao Mingquan,Latella Riccardo,Yuan Junhui,Liu Zheng,Wen Jiaxin,Ling Qinghao,Xu Yinsheng,Konstantinou Georgios,Lecoq Paul,Xia Mengling,Niu Guangda
Ultrafast radiation detection is crucial for advancing medical imaging, high-energy physics, astronomy, and industrial applications, offering high spatiotemporal resolution and reduced radiation exposure. However, the bottleneck lies in the formidable challenge of achieving scintillators with both ultrafast response and high efficiency. Contrasting with previous approaches that focused on molecular-level confinement, here we propose the strategy of pushing exciton confinement to the limit of atomic scale. Using 2D perovskites as a model, we design organic A-site cations to selectively enhance in-plane distortion to localize excitons, while suppressing out-of-plane and intra-octahedral distortions to minimize the formation of inefficient, long-lived self-trapped excitons. Specifically, (1,4-CMA)PbBr 4 exhibits a rare combination of fast response (0.62 ns) and high light yield (19,700 photons MeV −1 ), surpassing leading commercial and research scintillators. These properties enable breakthroughs in advanced imaging, including fast positron emission tomography with timing precision of 43.3 ps and high-resolution X-ray imaging (32 lp mm −1 ).
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Nature Communications
·
2025
·
IF 15.7
Na Qi,Zhang Xiaowang,Long Linna,Qi Siyu,Zhang Zhiyu,Zhi Ruoyu,Tan Zhuotao,Ying Hanjie,Zhu Chenjie
The textile dyeing industry has shaped modern aesthetics through vibrant coloration technologies. Indigoid dyes embody this potential but are hindered by the hazardous chemo-synthesis and water-intensive chemo-dyeing processes. While biosynthesis and bio-dyeing efforts offer alternatives, disjointed synthesis-dyeing steps lead to unsustainable water and energy demands, and complex biosynthetic pathways cause low atom economy and inefficiency. Here, we report a one-pot one-step approach that unifies biosynthesis and bio-dyeing of indigoids to drastically reduce water usage in the dyeing process. By combining alcohol dehydrogenase with flavin-containing monooxygenase, we construct an artificial redox-neutral cascade biosynthetic pathway to produce bluish-violet indigoid dyes, which significantly enhances atom economy and achieves high production efficiency. Various natural and synthetic fabrics are dyed in-situ with the biosynthetic indigoids utilizing recombinant E. coli whole cells. Crucially, this system dyes synthetic nylon fibers with the historic purple hue.
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