EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
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2024
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IF 4.3
Ziming Liu,Sihui Li,Pengfei Xu,Huiqin Gan,Pengfei Yue,Shuying Xie,Zixia Zhang,Weifeng Zhu,Zhiyu Guan
Objective To prepare chitosan-loaded nanoparticles (NPs) that enhance the oral bioavailability of puerarin (Pur) and render it responsive to reactive oxygen species (ROS). Significance This research makes substantial progress towards the theory of intelligent drug delivery, offering a new reference for combining Pur with other natural medicinal active ingredients. Methods The acylation reaction between chitosan and ROS-sensitive 3-carboxyphenylboronic acid (PBA) was used to synthesise ROS-sensitive phenylboronylated chitosan (PBACS). Subsequently, PBACS-PBA-Pur-NPs and PBACS-TPP-Pur-NPs were prepared via ion gelation after the addition of PBA and sodium tripolyphosphate(TPP), respectively. The physicochemical and functional properties of both NPs were compared, and their differences were preliminarily studied through molecular docking. Results Reactive oxygen species-sensitive PBACS was successfully synthesised. Of the two NPs prepared, PBACS-TPP-Pur-NPs had a size of 127.2 ± 0.80 nm, polydispersity index (PDI) of 0.129 ± 0.0008, and an encapsulation rate of 95.75 ± 0.387%, whereas PBACS-PBA-Pur-NPs had a size of 149.8 ± 0.1414 nm, PDI of 0.389 ± 0.0012, and an encapsulation rate of 91.77 ± 0.279%. The micromorphology of the PBACS-TPP-Pur-NPs exhibited better physical properties. However, PBACS-PBA-Pur-NPs demonstrated a faster in vitro release and more significant in vitro anti-inflammatory effects. Pharmacokinetically, the AUC 0-24 , T max , and C max of PBACS-PBA-Pur-NPs were 3.485, 2.117, and 3.339 times higher, respectively, than those of Pur. The AUC 0-24 , T max , and C max of PBACS-TPP-Pur-NPs were 2.41, 1.33, and 2.03 times higher, respectively, than those of Pur. Molecular simulation revealed that the binding energy of PBACS-PBA-Pur -NPs was approximately -4.34 kcal/mol and that of PBACS-TPP-Pur-NPs was even lower, approximately -5.93 kcal/mol, suggesting that the NPs prepared with TPP are more densely packed than those designed with PBA, resulting in slower and reduced drug release. Conclusion The NPs constructed in this study effectively reduced inflammatory factors at the disease site, providing a theoretical and experimental basis for the application of nano drugs in inflammatory disease models. In addition, the molecular docking study of the two NPs offered insights into the relationship between the release and structure of subsequent nano drugs.
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JOURNAL OF MEMBRANE SCIENCE
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2024
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IF 8.4
Yinyu Li,Cai Zhang,Jianqing Rao,Xiaogeng Lin,Yasan He
Covalent organic frameworks (COFs) based membranes have demonstrated great potential in molecular separation. However, COFs are usually synthesized as powders, and difficult to form large-area and highly crystalline membranes. There normally exists unfavorable crystal flaws and stacking defects in membranes, impairing their rejection performance. The severe reaction conditions of solvothermal synthesis also inhibit their fabrication. Here, a comprehensive strategy was developed to fabricate a homogeneously assembled COF membrane co-stitched by β-cyclodextrin (β-CD) and chitosan (CS). Specifically, the COF crystallites (named TATP) were homogeneously formed and assembled to be films at room temperature under the control of n-propylamine; The aminated CS chemically bonded the TATP crystallites on the substrate and helped assemble them to form dense films. β-CD molecules were subsequently embedded into the TATP layer and crosslinked to further stitch membrane defects. The prepared CD-TATP-NH 2 CS/nylon membrane demonstrated enhanced excellent rejection performance. It could reject 100 % of R250 (BB), Congo red, Direct blue15, and 99.4 % of Alcian blue. It also could selectively separate Methyl orange (MO) and BB mixed solutions with only MO passing through, owing to the co-stitching of the membrane defects by β-CD and CS. This study provides a significant concept and strategy for fabricating high-performance COF membranes.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Wenhui Chen,Jiayao Wang,Chiyin Zhang,Shaokui Cao,Jingguo Li,Jun Shi
Hollow natural polysaccharide microcapsules have broad applications in drug delivery field due to their excellent biocompatibility and drug loading efficiency. In this paper, pH/near-infrared (NIR) dual-responsive microcapsules composed of hyaluronic acid (HA), chitosan (CS) and hollow CuS (HA/CS/HA@CuS) had been fabricated via a layer-by-layer (LbL) approach. The negative charge, rough surface and hollow structure of microcapsules are very favorable for loading positively charged DOX. As a result, hollow microcapsules display a high drug loading efficiency of 91.15 %. The variation in the degree of amino ionization at different pH values leads to the changes in the electrostatic force between CS/HA multilayers, resulting in the structural change in microcapsules. Therefore, microcapsules exhibit significant pH-responsive drug release properties. In addition, hollow CuS nanoparticles with excellent photothermal conversion ability are capped on the multilayer surface, enabling microcapsules to exhibit excellent NIR-responsive drug delivery properties. Overall, hyaluronic acid/chitosan-based hollow microcapsules with notable pH/NIR dual-responsiveness have been prepared, which can be used as a potential drug carrier for controlled drug delivery and photothermal chemical combination therapy.
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CARBOHYDRATE POLYMERS
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2024
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IF 10.7
Yu Wang,Haojie Yu,Li Wang,Lei Zhang,Jinyi Liu,Dingning Chen,Jian Yang,Chenguang Ouyang,Jian Hu,Jingyi Feng,Chengjiang Li
Severely diabetic patients need insulin input to maintain the body's glycemic balance. However, traditional injection methods are often associated with poor adherence and an increased risk of hypoglycemia. Microneedle technology offers a promising solution by minimizing pain and trauma during insulin administration. Nonetheless, achieving prolonged glycemic control by microneedle with high insulin loading remains a significant challenge. Herein, we introduce an innovative microneedle patch that draws inspiration from the elegant light-induced blooming of water lily petals. The patch features a glucose-responsive hydrogel network crafted from two modified polysaccharide polymers, which enables the delivery of long-acting insulin without depending on glucose oxidase. By incorporating phenylboronic acid-modified sodium alginate, quaternary ammonium chitosan, and polyvinyl alcohol into a hydrogel matrix, we have created a microneedle system that harbors dynamic borate ester linkages and electrostatic attractions, resulting in heightened sensitivity to blood glucose levels. The electrostatic interaction acts as a relatively stable crosslinking point, balancing the dynamic reproducibility response based on the borate ester bond. This self-adaptive hydrogel can regulate insulin-controlled release by responding to changes in glucose concentration. Herein, we achieved massive insulin loading (20 IU) with long lasting glycaemic control (48 h) in a single treatment of diabetic SD rats.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Wei Su,Ying Zhang,Yiqing Chen,Lini Huo,Fangzhou Wen,Jinyun Cai,Qikun Gong,Peiyuan Li
Bacterial infections impede skin wound healing, and antibacterial hydrogels have garnered significant attention in the field of wound care due to their combined therapeutic effects. In this study, an intelligent, responsive AC-Gel@Cur-Au hydrogel was developed using temperature-sensitive agarose and pH-responsive chitosan as the structural framework, infused with Gel@Cur and AuNR. The AC-Gel@Cur-Au hydrogels demonstrated excellent mechanical properties, swelling capacity, tissue adhesion, and biodegradability. Upon irradiation, the hydrogels exhibited effective photodynamic therapy (PDT), photothermal therapy (PTT), and heat-sensitive gel-sol conversion capabilities, allowing for the release of curcumin in response to temperature and pH changes. Through these synergistic effects, the AC-Gel@Cur-Au hydrogel displayed a rapid bactericidal effect against Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ), effectively treating persistent biofilms. In vivo studies using a full-thickness defect model of S. aureus infection demonstrated that the hydrogel possessed strong antibacterial activity and enhanced wound healing capabilities, significantly reducing inflammation. Furthermore, the hydrogel significantly promoted granulation tissue formation, collagen deposition, and angiogenesis. Importantly, all the components of the AC-Gel@Cur-Au hydrogel were found to be non-cytotoxic, indicating good biocompatibility. This safe, synergistic antibacterial hydrogel holds great promise for the treatment of bacterial infections in wound healing.
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Nanomaterials
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2024
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IF 4.4
Xinrui Guo,Zhongxin Zhang,Zhanyan Liu,Hui Huang,Chunlei Zhang,Huaxin Rao
Non-fluorinated chitosan-based proton exchange membranes (PEMs) have been attracting considerable interest due to their environmental friendliness and relatively low cost. However, low proton conductivity and poor physicochemical properties have limited their application in fuel cells. In this work, a reinforced nanofiller (sulfonated CS/GO, S-CS/GO) is accomplished, for the first time, via a facile amidation and sulfonation reaction. Novel chitosan-based composite PEMs are successfully constructed by the incorporation of the nanofiller into the chitosan matrix. Additionally, the effects of the type and amount of the nanofillers on physicochemical and electrochemical properties are further investigated. It is demonstrated that the chitosan-based composite PEMs incorporating an appropriate amount of the nanofillers (9 wt.%) exhibit good membrane-forming ability, physicochemical properties, improved proton conductivity, and low methanol permeability even under a high temperature and low humidity environment. When the incorporated amounts of S-CS/GO are 9 wt.%, the proton conductivity of the composite PEMs was up to 0.032 S/cm but methanol permeability was decreased to 1.42 × 10 −7 cm 2 /s. Compared to a pristine CS membrane, the tensile strength of the composite membrane is improved by 98% and the methanol permeability is reduced by 51%.
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POLYMER
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2025
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IF 4.5
Peng He,Guanghui Gu,Youyin Xu,Gang Wei,Mingjin Xu
Skin wound healing faces challenges of bacterial infection and environmental stimulation, and hence the design of biocompatible wound dressings with antibacterial and self-healing abilities is promising. Herein, we report the design and synthesis of bioactive composite hydrogels (CHP) with chitosan (CS), hyaluronic acid (HA), and polypeptide (PP). Ascribing to dynamic molecular cross-linking, the fabricated CHP hydrogels exhibit good stability, excellent biocompatibility, injectability, self-healing ability, and antimicrobial activity. Microscopic analysis indicates that the CHP hydrogels maintain a 3D porous dual-network structure, and the porosity of hydrogels is related to the content of PP as the reinforcement. Antimicrobial evaluation of the CHP hydrogels demonstrates significant efficacy against E. coli and S. aureus , with improved performance at higher PP concentration. Furthermore, the hydrogels show enhanced cytocompatibility, minimal hemolysis, and improved in vitro cell proliferation. The tests with wound mouse models indicate that the CHP hydrogels can accelerate wound healing effectively, enhance angiogenesis, and promote tissue regeneration. Histological evaluation confirms complete epidermal regeneration, improved collagen deposition, and increased protein expression. The designed CHP hydrogels present potential clinical applications as a wound dressing material to meet the needs of rapid and effective wound healing.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Shuo Yang,Jianhao Liang,Nan Wang,Xiao-Kun Ouyang,Junhua Hu
Infected wounds are highly susceptible to bacterial contamination, which not only delays the healing process but also leads to a range of complications. NO is one of the most widely used gas molecules in wound treatment strategies due to its potent antibacterial and healing-promoting properties. However, excessive NO in infected wounds can damage cellular components and exacerbate inflammation. Therefore, precise control of NO dosage and targeted delivery to the wound site are crucial for effective treatment. Herein, a cationic hydrogel was constructed using modified guar gum and polyvinyl alcohol to enhance antibacterial effects, with guanidine-modified chitosan (Met-CS) and the photosensitizer sodium copper chlorophyllin (SCC) incorporated to create a light-triggered NO release system. Both in vitro and in vivo experiments confirmed that the proposed hydrogel exhibits excellent antibacterial performance, anti-inflammatory effects, and wound healing capabilities, demonstrating its potential for precise therapeutic applications.
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VACUUM
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2024
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IF 3.8
Difei Wang,Wei Chang,Zhiyuan Ren,Xiaojing Zhang,Yanpin Du,Bin Liu,Yunfeng Li
A novel visible-light-responsive ZnIn 2 S 4 /chitosan graphene aerogel (ZCGA) was prepared using a low-temperature hydrothermal self-assembly method for the adsorption and degradation of tetracycline. Infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS) indicated interactions between ZnIn 2 S 4 and chitosan graphene aerogel (CGA). Under the irradiation of a 500 W xenon lamp, ZCGA exhibited excellent adsorption and photocatalytic degradation performance for tetracycline, with a removal rate exceeding 90 % within 180 min. This significant performance improvement is attributed to the high carrier transport properties of CGA, which greatly enhanced the activity of the photocatalytic reaction and also showed high efficiency in removing other types of organic pollutants. Radical trapping experiments further confirmed that the superoxide anion (·O 2 − ) is the main active species in the photocatalytic process. In addition, the monolithic aerogel has good recyclability and mechanical stability. This study not only demonstrates the great potential of ZCGA in the field of photocatalysis but also provides new ideas for designing efficient, recyclable, and visible light-responsive photocatalysts.
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Alexandria Engineering Journal
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2025
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IF 6.8
Dong Li
This study investigated the development and application of TiO 2 /chitosan nanocomposite coatings for food preservation, focusing on their physicochemical properties and preservation efficacy. The nanocomposite synthesis achieved uniform particle dispersion with an average size of 85 ± 5 nm and zeta potential ranging from + 35.2 mV to + 42.8 mV. FTIR analysis revealed characteristic coordination bonds at 1384 cm-1 between Ti atoms and chitosan amino groups, while XRD patterns confirmed successful incorporation of TiO 2 nanoparticles without phase transformation. The optimal formulation (1.0 % TiO 2 ) demonstrated remarkable preservation effects, reducing respiratory activity by 42 % compared to controls and maintaining peroxidase activity 65 % lower than untreated samples. MDA levels in treated samples remained at 1.68 μmol/kg compared to 4.25 μmol/kg in controls after 15 days. The nanocomposite coating effectively suppressed microbial growth, maintaining total aerobic counts below 3.5 log CFU/g throughout storage, while preserving essential nutrients with 78 % retention of ascorbic acid content. The addition of thymol enhanced antimicrobial efficacy, generating inhibition zones of 18.5 mm against E. coli. The coating's stability was maintained over 30 days with less than 5 % variation in particle size distribution, demonstrating its potential for commercial food preservation applications.
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DRUG DELIVERY
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2012
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IF 1.456
Ning Li,Chun-Yang Zhuang,Mi Wang,Cheng-Guang Sui,Wei-San Pan
In this study, low molecular weight chitosan coated liposomes (LCHL) were designed and prepared for ocular drug delivery, the coating mechanism was studied, and in vitro and in vivo characterization was conducted. The effects of molecular weight and concentration of low molecular weight chitosan on the liposomal coating were studied. The numeric relations between coating variables and coating efficiency were established using a mathematical model. Morphology of LCHL was examined by transmission electron microscopy (TEM). Cytotoxicity and cell internalization of FITC-BSA labeled LCHL in a rabbit conjunctival epithelium (RCE) cell line were studied. Cyclosporin A (CsA) was encapsulated as a model drug, and in vitro drug release and in vivo drug absorption were investigated. LCHL demonstrated low toxicity to RCE cells. In vitro drug release measurement showed that LCHL had a delayed release profile compared with non-coated liposomes. In vivo study in rabbits showed that the concentrations of CsA in cornea, conjunctiva, and sclera were remarkably increased by LCHL. In conclusion, LCHL might be a potential ocular drug carrier with characteristics such as prolonged drug retention, enhanced drug permeation, and biocompatibility.
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POLYMER DEGRADATION AND STABILITY
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2011
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IF 2.594
Qin Chen,Wenjun Xiao,Lilong Zhou,Tinghua Wu,Ying Wu
Hydrolysis of chitosan in ionic liquids was carried out under microwave irradiation (MW) using sulfonic acid-functionalized ionic liquids (SFILs) as catalysts. The effect of microwave power, irradiation time, dosage of SFILs and DMSO was investigated by orthogonal tests. Under the optimal reaction conditions, the yield of total reducing sugars (TRS) reached over 90% within 2 min. The viscosity-average molecular weight of degraded chitosan was determined by viscosity method. The structures of the original and degraded chitosan were characterized by Fourier-transform infrared (FTIR) spectra, X-ray powder diffraction (XRD) analysis and carbon-13 nuclear magnetic resonance spectroscopy ( 13 C NMR). The influence of microwave power and irradiation time on the TRS and M v was further studied. This method can dramatically reduce reaction time.
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