AQUACULTURE NUTRITION
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2023
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IF 3.5
Jianxin Zhang,Mengyuan Huang,Junchang Feng,Yongyan Chen,Meng Li,Xiaolin Meng,Xulu Chang
This study is aimed at exploring the effects of Bacillus coagulans NRS 609 on the growth performance, intestinal health, antioxidant activity, and immune response in common carp (Cyprinus carpio L.) by colonizing the intestinal tract with the beneficial strain. Gavage B. coagulans with GFP-tag at 109 CFU/mL into common carp for 7 days. Results indicated that B. coagulans were maintained in the intestine for at least 14 days. Four groups fed B. coagulans at different levels of inclusion, including 0 (CK), (A), (B), and (C) CFU/g of feed for 8 weeks. Experimental groups showed significantly better growth performance than the control (). The treatment groups had higher digestive enzyme activity, villus height, and muscle thickness than the control (). Supplementation with B. coagulans also altered the composition of the intestinal flora. In addition, an enhancement of the antioxidant enzyme and a reduction of malondialdehyde in the liver were observed with the addition of B. coagulans (). Moreover, the cytokines of IL-1β and IL-12, IL-10, and TGF-β, significant upregulation in experimental groups (). Based on the results, the optimal level of B. coagulans was 107 CFU/g in diets for common carp.
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Gels
·
2022
·Vol. 8
·期 5
·279
·
IF 4.432
Hang Yang,Xianyu Lan,Yuzhu Xiong
Although many advances have been made in medicine, traumatic bleeding and wound infection are two of the most serious threats to human health. To achieve rapid hemostasis and prevent infection by pathogenic microbes, the development of new hemostatic and antibacterial materials has recently gained significant attention. In this paper, safe, non-toxic, and biocompatible polyvinyl alcohol (PVA); carboxymethyl cellulose (CMC), which contains several carboxyl and hydroxyl groups; and polyethylene glycol (PEG), which functions as a pore-forming agent, were used to prepare a novel PVA/CMC/PEG-based composite hydrogel with a macroporous structure by the freeze-thaw method and the phase separation technique. In addition, a PVA/CMC/PEG@ZIF-L composite hydrogel was prepared by the in situ growth of zeolitic imidazolate framework-L (ZIF-L). ZIF-L grown in situ on hydrogels released Zn2+and imidazolyl groups. They elicited a synergistic antibacterial effect in hemostasis with PVA and CMC, rendering the PVA/CMC/PEG@ZIF-L hydrogel with a good antibacterial effect againstStaphylococcus aureus. At the same time, the macroporous structure enabled the rapid release of Zn2+and imidazolyl groups in ZIF-L and promoted cell proliferation at an early stage, enhancing the coagulation efficiency. A rat liver injury model was used to confirm its rapid hemostasis capacity.Keywords:in situ growth;macroporous structure;synergistic antibacterial;rapid hemostatic;carboxymethyl cellulose;wound dressing;hydrogel;ZIF-L;polyvinyl alcohol;phase separation technique
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Food Science & Nutrition
·
2021
·Vol. 10
·期 2
·617-630
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IF 2.863
Xin Zhao,Zhifei Fu,Minghe Yao,Yu Cao,Tongtong Zhu,Rui Mao,Ming Huang,Yafen Pang,Xianghui Meng,Lin Li,Boli Zhang,Yuhong Li,Han Zhang
Dietary supplements are currently being used to ameliorate metabolic alterations via maintaining gut microflora balance. Mulberry leaf is known as medicine homologous food for its glucose- and lipid-modulating properties. However, the effects of mulberry leaf polysaccharide (MP) on metabolic dysbiosis and gut microbiota are still poorly understood. After extraction and characterization, the beneficial effects of water-soluble MP were evaluated in high-fat diet-induced obese mice. MP treatment could reduce adipose tissue, improve insulin resistance, and alleviate the pathological lesions in colon. Investigation of the underlying mechanism showed that MP modulated gut microbial community by 16S rRNA analysis and reversed the elevation of lipid indexes by plasma lipidomics analysis. Correlation analysis indicated that the abundance of seven key bacterial species and six lipids were closely associated with the metabolic traits, respectively. Overall, MP could ameliorate metabolic disorders, and modify the gut microbiota and lipids. This would greatly facilitate the utilization of MP as a functional food.
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JOURNAL OF ETHNOPHARMACOLOGY
·
2023
·
IF 5.4
Danilo de Souza Costa,Carla Monteiro Leal,Rayssa A. Cajas,Matheus Coutinho Gazolla,Lívia Mara Silva,Lara Soares Aleixo de Carvalho,Bruna L. Lemes,Renato Oliveira de Moura,Juliana de Almeida,Josué de Moraes,Ademar A. da Silva Filho
Ethnopharmacological relevance Roots of Pothomorphe umbellata (L.) Miq. are used in traditional medicine of Africa and South America for the treatment of malaria and helminthiasis . However, neither P. umbellata nor its isolated compounds have been evaluated against Schistosoma species. Aims of this study To investigate the antischistosomal effects of P. umbellata root extracts and the isolated compound 4-nerolidylcatechol (4-NC) against Schistosoma mansoni ex vivo and in murine models of schistosomiasis. Materials and methods The crude hydroalcoholic (PuE) and hexane (PuH) extracts of P. umbellata roots were prepared and initially submitted to an ex vivo phenotypic screening against adult S. mansoni . PuH was analyzed by HPLC-DAD, characterized by UHPLC-HRMS/MS, and submitted to chromatographic fractionation, leading to the isolation of 4-NC. The anthelmintic properties of 4-NC were assayed ex vivo against adult schistosomes and in murine models of schistosomiasis for both patent and prepatent S. mansoni infections. Praziquantel (PZQ) was used as a reference compound. Results PuE (EC 50 : 18.7 μg/mL) and PuH (EC 50 : 9.2 μg/mL) kill adult schistosomes ex vivo . The UHPLC–HRMS/MS analysis of PuH, the most active extract, revealed the presence of 4-NC, peltatol A, and peltatol B or C. After isolation from PuH, 4-NC presented remarkable in vitro schistosomicidal activity with EC 50 of 2.9 μM (0.91 μg/mL) and a selectivity index higher than 68 against Vero mammalian cells, without affecting viability of nematode Caenorhabditis elegans . In patent S. mansoni infection, the oral treatment with 4-NC decreased worm burden and egg production in 52.1% and 52.3%, respectively, also reducing splenomegaly and hepatomegaly . 4-NC, unlike PZQ, showed in vivo efficacy against juvenile S. mansoni , decreasing worm burden in 52.4%. Conclusions This study demonstrates that P. umbellata roots possess antischistosomal activity, giving support for the medicinal use of this plant against parasites. 4-NC was identified from P. umbellata roots as one of the effective in vitro and in vivo antischistosomal compound and as a potential lead for the development of novel anthelmintics.
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Frontiers in Chemistry
·
2021
·
IF 5.221
Zhang Zhikun,Zhao Wenmeng,Hu Cuixia,Cao Yapeng,Liu Yumin,Liu Qingju
L-Histidine (L-His) is an essential amino acid, which is used to synthesize proteins and enzymes. The concentration of L-His in the body is controlled to regulate tissue growth and repair of tissues. In this study, a rapid and sensitive method was developed for colorimetric L-his detection using Cu2+ ions to inhibit the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB)–H2O2 system. H2O2 can oxidize TMB to oxTMB in the presence of copper, and the change in color from colorless (TMB) to blue (oxTMB) is similar to that observed in the presence of peroxidase. However, because the imidazole ring and carboxyl group of L-His can coordinate with Cu2+ ions to form stable L-His–Cu2+ complexes, the color of the TMB–H2O2 solution remains unchanged after the addition of L-His. Therefore, because L-His effectively hinders the colorimetric reaction of TMB with H2O2, this assay can be used to quantitatively determine the concentration of L-His in samples. Under optimized conditions, our colorimetric sensor exhibited two linear ranges of 60 nM to 1 μM and 1 μM to 1 mM for L-His detection and a detection limit of 50 nM (S/N = 3); furthermore, the assay can be performed within 20 min. Moreover, the proposed assay was used to determine the concentration of L-His in urine samples, suggesting that this convenient and label-free colorimetric method presents promising applications in bioanalytical chemistry and clinical diagnosis.
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2021
·
IF 4.952
Han Wu,Hao-Nan Liu,Ai-Min Ma,Jian-Zhong Zhou,Xiu-Dong Xia
In this study, Lactobacillus plantarum strains, namely 70810, B1-6, and ZR were used to confirm the synergetic effects of co-inoculation with Lactobacillus and Rhizopus oryzae on whole-oat solid state fermentation (SSF). The results indicated that number of viable bacterial cells increased more quickly (10.72–11.23-fold higher) during co-inoculated SSF than that during pure Lactobacillus fermentation. Fungal ergosterol content reached slightly less during co-inoculation (137.04–155.12 mg/kg) than that during R. oryzae fermentation (193.64 mg/kg). However, compared to R. oryzae , R. oryzae combined with Lactobacillus produced more obvious hydrolysis of macromolecules and accelerated more water transformation from confined to free form. As for co-inoculated SSF of oat, the reducing sugar content reached the highest at 41.09–47.42 g/100g and then dropped, and free amino acid nitrogen content varied gradually until 40.66–41.67 mg/100g. Fermented oats (FOs) had higher total phenolic content than unfermented oat (UO) (2.56–2.72-fold, FO vs . UO), and their DPPH radical scavenging activity, ferric reducing capacity, and hydroxyl scavenging activity were increased 2.04-, 2.10-, and 1.99-fold at maximum (FO vs . UO), respectively. The Lactobacillus showed strain dependence during co-inoculated SSF, among which B1-6 displayed the best to enhance oat's overall quality.
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CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Ziyuan Qiao,Jian Zhang,Chang Zhou,Feng-Shou Xiao
The development of efficient Beta zeolite for conversion of L-lactic acid (L-LA) to L,L-lactide (L-LT) is in urgent need but still remains challenging. Herein, we reported an enhancement of the L-LT yield by passivation of external acidic sites on aluminosilicate Beta zeolites by coating pure silica Beta (Al-Beta@Si-Beta). The passivation of external acidic sites was strongly evidenced by in situ 2,4,6-tritertbutylpyridine (TTBP) adsorption FT-IR spectra, where the external acidic sites of Al-Beta@Si-Beta were effectively covered. In the tests for conversion of L-LA to L-LT, the Al-Beta@Si-Beta exhibited much higher L-LT yield than commercial microporous Beta zeolite, which is reasonably attributed to that the passivation of external acidic sites of the Beta zeolite significantly decreases molecular weight of the reaction intermediates, favoring for reduction of coke formation. Furthermore, this strategy is extended to passivate mesoporous Beta zeolite (Al-Beta-meso@Si-Beta). As expected, the Al-Beta-meso@Si-Beta displayed higher L-LT yield than the Al-Beta@Si-Beta because the presence of mesoporosity in the zeolite catalyst is favorable for mass transfer in the conversion of L-LA to L-LT. The strategy for coating pure siliceous zeolite might offer a new opportunity to develop highly efficient zeolite catalysts for the production of L-LT in the future.
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ENVIRONMENTAL POLLUTION
·
2023
·
IF 8.9
Xiaojing Li,Qian Wu,Dan Chen,Yichen Bai,Yuhong Yang,Shiwen Xu
Cadmium (Cd) is a major contaminant in natural environments and exerts adverse effects on aquatic biota at low concentrations. Gill is as vital respiratory organ and may cause pollutants to enter fish during gas exchange. Baicalein (BAI), as a kind of flavonoids, possess antioxidant properties through inactivating free radicals. To confirm the potential effects and approaches of BAI addition in maintaining the gill stability, 90 common carps ( Cyprinus carpio L.) were selected and randomly divided into water environment exposure group (0.22 mg/L Cd) and/or feed added with 0.10 g/kg BAI for 30 days. The analysis of ion content in serum showed that Cd exposure disturbed ion homeostasis , and BAI could reduce serum Cd concentration. The histopathological results of gills showed that Cd exposure caused gill tissue lesions and structural damage, and BAI feeding effectively alleviated this damage. In addition, BAI could enhance antioxidant activity and activate Nrf2/HO-1 axis, thereby reducing oxidative stress and endoplasmic reticulum (ER) stress. Moreover, BAI lightened cytokine imbalance, inflammatory response, and necroptosis. Overall, the results indicated that BAI feeding could maintain gill homeostasis against Cd poisoning via the ROS/ER stress signaling. This trial revealed the properties of BAI resistance to metal Cd in aquaculture and partially elucidated its mechanism.
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FOOD HYDROCOLLOIDS
·
2023
·
IF 10.7
Yamei Wu,Yanyan Wu,Huan Xiang,Shengjun Chen,Yongqiang Zhao,Qiuxing Cai,Yueqi Wang
In this study, we aimed to investigate the influence of lysine-assisted ultrasonic treatment on the conformation, and interfacial and emulsification properties of sea bass myofibrillar proteins at the oil–water interface. It was observed that the modification treatment with lysine (1 g/L) combined with low-intensity ultrasound (200 W) enhanced the interfacial pressure and penetration rate. This enhancement could be attributed to the significant structural unfolding of myofibrillar proteins located at the oil–water interface (increase in β-sheets and reduction in α-helices), which exposed the hydrophobic groups. The rate of interfacial protein adsorption subsequently increased, owing to the strengthening of protein–protein and protein–oil phase interactions. Additionally, the interfacial swelling rheology experiment showed an enhancement in the ordered structure of interfacial proteins, aiding in the development of a protein gel network structure. Consequently, the elastic modulus of the oil–water interface was larger, and the droplet interfacial structure was tight, which facilitated uniform distribution and improved the emulsification properties of the droplets, ultimately enhancing the ability of the emulsions to be stored at room temperature.
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FOOD CHEMISTRY
·
2021
·
IF 7.514
Weiqiang Li,Kaisheng Diao,Daoyang Qiu,Yifang Zeng,Kaijie Tang,Yifu Zhu,Yingying Sheng,Yangping Wen,Mingfang Li
A new strategy to mimic antibody for electrochemical recognition and detection of deoxynivalenol (DON) using a highly-sensitive and selective antibody-like sensor based on molecularly imprinted poly( l -arginine) (P-Arg-MIP) on carboxylic acid functionalized carbon nanotubes (COOH-MWCNTs) was proposed. l -arginine as functional monomer was screened to prepare imprinted electrode via its electro-polymerization in the presence of DON onto the surface of COOH-MWCNTs electrode coupled with theoretical calculation. Surface morphology, structural characteristics, and electrochemical properties of P-Arg-MIP/COOH-MWCNTs were characterized by SEM, EDS, FTIR, and CV, respectively. P-Arg-MIP/COOH-MWCNTs displayed relatively high conductivity, high effective surface area, antibody-like molecular recognition and affinity, and a good response towards DON in a linear range from 0.1 to 70 μM with LOD of 0.07 μM in wheat flour samples with satisfactory recovery and feasible practicability in comparison with HPLC. This method provides a promising biomimetic sensing platform for the determination of mycotoxins in food and agro-products.
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Journal of Functional Foods
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2020
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IF 3.701
Shan Shan Qi,Meng Li Shao,Sun Ze,Hong Xing Zheng
Salidroside from Rhodiola rosea L. has hypoglycemic effects, and has anti-osteoporosis effect in diabetic rats. Whether it has beneficial effects on diabetic nephropathy (DN) has not been fully reported. Here, a DN rat model was established by streptozotocin (STZ). Salidroside (10, and 20 mg kg −1 d −1 ) were orally giving for eight weeks. The purity of salidroside was 99%. The results showed that eight weeks salidroside supplementation dose-dependently decreased blood glucose, serum insulin, serum creatinine, blood urea nitrogen (BUN), as well as urinary albumin of DN rats, repaired the pathological changes of kidney tissue, the renal sclerosis and fibrosis in the kidney of DN rats were improved, and the activities of oxidative defense system associated enzyme such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) were also improved. Serum inflammatory cytokines (including tumor necrosis factor-α, monocyte chemoattractant protein-1, interleukin-1β, and interleukin-6) were decreased significantly in DN rats after eight weeks salidroside supplementation. Immunohistochemical results showed that fibrosis related proteins such as transforming growth factor-β1 (TGF-β1), phospho-Smad2 (p-Smad2), and phospho-Smad3 (p-Smad3) expression levels were decreased significantly in the kidney of salidroside treated group. Our results indicate that daily salidroside consumption could alleviate DN in rats, the main mechanisms are anti-inflammatory, anti-oxidative stress, and inhibition of the TGF-β1/Smad2/3 pathway. This study suggested that salidroside could be used as a nutrient against DN, as well as diabetes.
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BIORESOURCE TECHNOLOGY
·
2020
·
IF 7.539
Haiwei Jiang,Liangliang Fan,Chang Cai,Ying Hu,Fenghua Zhao,Roger Ruan,Weiran Yang
The hydrothermal liquefaction (HTL) of As enriched Pteris vittata L. (PVL, hyper-accumulator biomass) was performed with the recycled aqueous phase as reaction medium, aiming to dispose the biomass with high water content and produce high-quality bio-oil. After three times of aqueous phase recycling at 275 °C, 30 min, the bio-oil yield increased to 30.32% from 21.54% and the higher heating value (HHV, 28.51 MJ/kg) of the bio-oil was higher than that of the bio-oil from HTL with pure water (26.80 MJ/kg). The main compounds detected in bio-oils were phenols, ketones, hydrocarbons, and aldehydes. Acetic acid (17.21–24.77 mg/mL) was dominant in the aqueous phases, resulting in the low pH (4.31–4.89). The heavy metals (Cu, Pb, Zn, Cd) mainly remained in bio-char whereas As was transferred to aqueous phase. Thus, HTL by aqueous phase recycling could be a promising way for PVL treatment to obtain high-quality bio-oil and arsenic recovery.
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