JOURNAL OF HAZARDOUS MATERIALS
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2019
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IF 7.65
Zhanghui Wang,Shuang Guo,Bin Zhang,Jinchao Fang,Liping Zhu
Persistent organic micropollutants have seriously damaged aquatic ecological equilibrium and affected human health. Conventional adsorbents are limited due to slow adsorption rate. Therefore, it’s significant to integrate adsorbent into porous membrane to develop a highly efficient continuous filtration method for water purification. Herein, β-cyclodextrin polymer (β-CDP) composite porous membranes were prepared via convenient interfacial cross-linking. The membranes combined the adsorption ability of β-CDP and the convective mass transport process of filtration membrane to quickly remove contaminants from water by flow-through adsorption. In optimized preparation conditions, the composite membrane exhibited a 100% of removal efficiency towards bisphenol A and a high treating capacity up to 440 mg m -2 . The treating capacity kept nearly unchanged in acidic and neutral pH condition, while increased greatly with the addition of salts due to the salting-out effect. Also, the membrane could completely remove pollutants with ultrahigh flux up to 2500 L·m -2 h -1 . In addition, the used membranes were fully regenerated by mild ethanol cleaning.
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
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2019
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IF 2.606
Qi Lan,Donghua Di,Siling Wang,Qinfu Zhao,Yikun Gao,Di Chang,Tongying Jiang
The aim of this study was to develop a novel ocular drug delivery system based on the hydroxypropyl beta cyclodextrin (HP-β-CD) inclusion complex coated with the synthesized chitosan-N-acetyl- l -cysteine conjugate (CS-NAC) for the hydrophobic drug quercetin . The inclusion complex was prepared by the solvent evaporation method, and the concentration of HP-β-CD and CS-NAC was optimized by isolated corneal penetration test. The optimized concentration of HP-β-CD and CS-NAC was 20% (w/v) and 0.1% (w/v), respectively. The CS-NAC/quercetin-HP-β-CD had the effect on improving the ocular penetration with little ocular irritation and could increase the ocular distribution significantly. Meanwhile, Coumarin-6 (C6) labelled CS-NAC/C6-HP-β-CD possessed enhanced fluorescence and located deeper into the epithelium of cornea at the same time point compared with the uncoated one. It could also transport drug through the corneal epithelium by intracellular routes except for intercellular routes. Furthermore, it could delay the occurrence of cataract about 1 d when administrated with full-dosage quercetin. The HP-β-CD inclusion complex coated with CS-NAC is a safe and promising ocular drug delivery system for improving the bioavailability and corneal penetration of hydrophobic drug like quercetin.
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ANALYST
·
2019
·Vol. 145
·期 2
·619-625
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IF 4.019
Nuo Zhang,Yaoguang Wang,Guanhui Zhao,Chao Wang,Yueyuan Li,Yong Zhang,Huan Wang,Qin Wei
An ultrasensitive label-free photoelectrochemical (PEC) immunosensor was developed to detect amyloid β-protein (Aβ) based on CdS/CdTe-cosensitized SnO2 nanoflowers. Specifically, SnO2 with a flower-like porous nanostructure was utilized as a perfect substrate for the construction of PEC immunosensors, and the SnO2-modified electrode was first coated with CdTe quantum dots (QDs) and then further deposited with CdS by successive ionic layer adsorption and reaction techniques. The formed SnO2/CdS/CdTe-cosensitized structure exhibited excellent photocurrent intensity and was employed as an excellent photoactive matrix to immobilize Aβ antibody to further construct the immunosensor. Under optimal conditions, the as-constructed PEC immunosensor was used to detect Aβ and exhibited a wide linear concentration range from 0.5 pg mL−1 to 10 ng mL−1, with a low limit of detection (LOD, 0.18 pg mL−1, S/N = 3). Meanwhile, it also presented good reproducibility, specificity, and stability and may open a new promising platform for the clinical detection of Aβ or other biomarkers.
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RSC Advances
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2019
·Vol. 9
·期 52
·30249-30258
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IF 3.049
Feng Yang,Yu Liu,Tong Liu,Shuan Liu,Haichao Zhao
In this work, a water-soluble host–guest complex containing carboxymethylated beta-cyclodextrin (CM-β-CD) and an aniline trimer (AT) was synthesized. The application of AT–CM-β-CD as an inhibitor for alleviating the corrosion of Q235 carbon steel in 1 M HCl solution was investigated by potentiodynamic polarization and electrochemical impedance spectroscopy (EIS). Results showed that the inhibition efficiency was significantly increased in the presence of AT–CM-β-CD, and the inhibition efficiency was up to 99.2% when the concentration of AT–CM-β-CD was 250 mg L−1. Field emission scanning electron microscopy (SEM) and laser scanning confocal microscopy (LSCM) confirmed that the corrosion inhibitor had excellent corrosion inhibition effects due to the formation of an adsorption film on the surface of Q235 carbon steel. According to the data extracted from the Langmuir adsorption model, AT–CM-β-CD absorption involves both physisorption and chemisorption.
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CHEMICAL ENGINEERING JOURNAL
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2022
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IF 16.744
Guo Wen Sun,Meng Jing Jin,Qian Yu Liu,Chao Yue Zhang,Jiang Long Pan,Yan Chun Wang,Xiu Ping Gao,Geng Zhi Sun,Xiao Jun Pan,Jin Yuan Zhou
Electric field from ferroelectric materials was often applied to energy-storage devices to enhance their electrochemical reaction kinetics via the electrostatic interaction. However, traditional ferroelectric materials are difficult to be used commercially for their insulation. Herein, we elucidate a novel strategy to introduce the electrostatic interaction by polarizing the polyvinylidene fluoride (PVDF) binder used in the C/S cathodes for lithium-sulfur batteries. In particular, we show both experimentally and theoretically how an external electric field from β -phase PVDF induces unprecedented performance and stabilities. Density functional theory calculation results indicate that the polarized topological β -phase PVDF possesses not only an strong built-in electric field and an improved conductance that benefits both the deposition of LiPSs and the transport of Li + ions, but also an enhanced adsorption capability to polysulfide ions. As a result, the specific capacity was improved by over 60 %, the rate capability (from 0.1 to 2.0 C) was enhanced by 138 %, and the capacity decay rate (1000 cycles at 1.0 C) largely decreases from 0.059 % to 0.038 % per cycle. Besides, the enhancement mechanism of Li 2 S deposition by electrostatic interaction has also been explored. This research would provide some new insight into the ferroelectric enhanced design of high-performance energy-storage devices.
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EUROPEAN JOURNAL OF PHARMACOLOGY
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2026
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IF 5.7
Nana Long,Youwei Zuo,Jian Li,Renxiu Yao,Xiao Zhang,Hongping Deng
Background Atopic dermatitis (AD) is characterized by epidermal barrier impairment and immune-driven inflammation. Strategies that restore barrier architecture while constraining inflammation are of therapeutic interest. Purpose To evaluate the barrier-reparative and anti-inflammatory effects of β-myrcene (MYR) in experimental atopic dermatitis and to investigate whether ECM–FAK/Src–YAP signaling is associated with these effects. Methods A 2,4-dinitrochlorobenzene (DNCB)-induced murine model of AD-like dermatitis was evaluated by transepidermal water loss (TEWL), histopathology, dermal collagen organization, filaggrin (FLG) immunofluorescence, and circulating inflammatory mediators. Integrated skin transcriptomic and proteomic analyses and public human AD transcriptomic datasets were used to identify MYR-associated pathways. In primary human keratinocytes stimulated with tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ), YAP localization, FAK Tyr397 and Src Tyr416 phosphorylation, YAP/TAZ target genes, barrier markers, and inflammatory outputs were assessed. Peptide 17 was used to examine the contribution of YAP-dependent transcription. Results MYR reduced dermatitis severity and epidermal hyperplasia, lowered TEWL, partially restored FLG immunofluorescence, and preserved dermal collagen organization in DNCB-challenged mice. Multi-omics profiling identified MYR-associated changes in extracellular matrix (ECM)–receptor interaction, focal adhesion, cytoskeletal remodeling, epidermal differentiation, and immune-related programs. In inflamed keratinocytes, MYR was associated with increased FAK and Src phosphorylation, YAP nuclear accumulation, CCN1/CCN2 expression, and barrier-marker expression, together with reduced inflammatory mediator production. Peptide 17 attenuated MYR-associated YAP nuclear accumulation and several downstream transcriptional responses. These findings support the participation of YAP-dependent signaling but do not establish MYR as a direct regulator of FAK, Src, or YAP or demonstrate a strictly linear signaling sequence. Conclusion MYR produced barrier-restorative and anti-inflammatory effects in experimental atopic dermatitis. The results identify ECM–FAK/Src–YAP signaling as a candidate pathway associated with the MYR response, with pharmacological inhibition providing partial functional evidence for a YAP-dependent component. Download: Download high-res image (265KB) Download: Download full-size image
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Journal of Biological Engineering
·
2026
·
IF 6.3
Zhang Zhi,Li Xue,Tang Teng Hua,Yang Wan Jia,Lu Qin Hui,Qiu Yan Yan,Wu Cheng Xin
Background Poly(β-L-malic acid) (PMA) is a biodegradable, biocompatible, and chemically modifiable polymer with broad applications. However, the production of high-molecular-weight, high-purity PMA (hhPMA) has long been constrained by “upstream–downstream linkage obstacles”, resulting in relatively low yield, high cost, and significant environmental burden. To address this issue, we systematically reconstructed the hhPMA production chain, establishing an integrated green chain covering the upstream, midstream, and downstream stages. Results In the upstream stage, we isolated a high-performing strain ( Aureobasidium pullulans 389) for hhPMA from Hevea brasiliensis , which inherently produces high titers of PMA with a dominant high-molecular-weight fraction, and systematically engineered it by reducing by-product biosynthesis, enhancing precursor supply, and balancing cofactors, thereby improving PMA synthesis further. In the midstream stage, a mixed-substrate strategy combining refined sugars with H. brasiliensis sawdust hydrolysate reduced refined carbon and nitrogen inputs by ~ 85% and ~ 100% in shake flasks, and achieved a PMA titer of ~ 260 g/L in a 5-L bioreactor. In the downstream stage, a green separation process integrating CaCO₃ recycling, anion-exchange resin adsorption, and ultrafiltration achieved an overall PMA recovery of > 90%, with > 95% recovery and nearly 99% purity for the ≥ 20 kDa fraction, minimizing Ca-containing wastewater and eliminating solvents. Conclusions This study systematically reconstructed the hhPMA production chain through coordinated upstream strain engineering, midstream mixed-substrate fermentation, and downstream green purification. The reconstructed production chain overcomes “upstream–downstream linkage obstacles,” enabling a more efficient, economical, and green hhPMA manufacturing process from lignocellulosic biomass of H. brasiliensis wood. These findings offer a valuable reference for the production of hhPMA and related organic acids/biopolymers in A. pullulans .
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NEUROSCIENCE
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2026
·
IF 3.3
Xiaoyu Lin,Wanliang Du,Ruile Fang,Ying Ma,Yongjun Wang,Jialei Yang
Systemic inflammation worsens ischemic stroke by increasing brain injury, neurological deficits, and neuroinflammation. • Systemic inflammation enhances microglial NF-κB activity and promotes p65/β-catenin interaction. • Systemic inflammation downregulates microglial Wnt/β-catenin/CD36 axis and impairs microglial efferocytosis. • Lithium chloride restores Wnt/β-catenin signaling and microglial efferocytosis, improving stroke outcome under systemic inflammation. Systemic inflammation is associated with worse outcomes after ischemic stroke, but the underlying mechanisms remain poorly understood. Microglial efferocytosis limits post-stroke inflammation and promotes recovery, whereas Wnt/β-catenin signaling has been implicated in neuroprotection and in regulation of CD36, a key efferocytotic receptor. Here, we investigated whether systemic inflammation is associated with alterations in microglial Wnt/β-catenin/CD36 axis and efferocytosis during ischemic injury. Mice were pretreated with lipopolysaccharide (LPS) before transient middle cerebral artery occlusion/reperfusion (tMCAO/R), and BV2 microglia were exposed to LPS before oxygen-glucose deprivation/reoxygenation (OGD/R). LPS pretreatment markedly increased infarct volume, worsened neurological deficits, and amplified post-ischemic neuroinflammatory responses. These effects were accompanied by enhanced microglial NF-κB signaling, increased p65/β-catenin interaction, and reduced nuclear β-catenin, consistent with suppression of Wnt/β-catenin signaling. Reduced CD36 expression and impaired microglial efferocytosis were observed in parallel. Notably, pharmacological activation of Wnt/β-catenin signaling with lithium chloride (LiCl) restored CD36 expression and microglial efferocytosis under LPS-pretreated conditions, and was accompanied by smaller infarcts, improved neurological function, and attenuated neuroinflammation. Collectively, these findings show that LPS pretreatment exacerbates ischemic stroke and is associated with enhanced microglial NF-κB signaling, dysregulation of the Wnt/β-catenin/CD36 axis, and impaired microglial efferocytosis. These results support defective microglial efferocytosis as a potential biological link between systemic inflammation and aggravated ischemic injury and identify Wnt/β-catenin signaling as a candidate pathway for further therapeutic investigation Download: Download high-res image (128KB) Download: Download full-size image
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JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
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2023
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IF 5.3
Xiaojing Xing,Meichen Liu,Xuguang Wang,Qianxue Guo,Hongyue Wang,Wenxue Wang
Diffuse large B-cell lymphoma (DLBCL) is difficult to treat due to the high recurrence rate and therapy intolerance, so finding potential therapeutic targets for DLBCL is critical. FK506-binding protein 3 (FKBP3) contributes to the progression of various cancers and is highly expressed in DLBCL, but the role of FKBP3 in DLBCL and its mechanism are not clear. Our study demonstrated that FKBP3 aggravated the proliferation and stemness of DLBCL cells, and tumour growth in a xenograft mouse model. The interaction between FKBP3 and parkinsonism associated deglycase (PARK7) in DB cells was found using co-immunoprecipitation assay. Knockdown of FKBP3 enhanced the degradation of PARK7 through increasing its ubiquitination modification. Forkhead Box O3 (FOXO3) belongs to the forkhead family of transcription factors and inhibits DLBCL, but the underlying mechanism has not been reported. We found that FOXO3 bound the promoter of FKBP3 and then suppressed its transcription, eventually weakening DLBCL. Mechanically, FKBP3 activated Wnt/β-catenin signalling pathway mediated by PARK7. Together, FKBP3 increased PARK7 and then facilitated the malignant phenotype of DLBCL through activating Wnt/β-catenin pathway. These results indicated that FKBP3 might be a potential therapeutic target for the treatment of DLBCL.
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BIOCHEMICAL PHARMACOLOGY
·
2023
·
IF 5.8
Wen-long Zhao,Di Xu,Jun-song Wang
Aldose reductase (AR) is an important enzyme involved in the reduction of various aldehyde and carbonyl compounds, including the highly reactive and toxic 4-hydroxynonenal (4-HNE), which has been linked to the progression of various pathologies such as atherosclerosis , hyperglycemia , inflammation, and tumors. AR inhibitors have potential therapeutic benefits for these diseases by reducing lipid peroxidation and mitigating the harmful effects of reactive aldehydes. In this study, we found that torachrysone-8- O -β- d -glucoside (TG), a natural product isolated from Polygonum multiflorum Thunb., functions as an effective inhibitor of AR, exhibiting potent effects in clearing reactive aldehydes and reducing inflammation. TG up-regulated the mRNA levels of several antioxidant factors downstream of NRF2, especially glutathione S-transferase (GST), which is significantly increased, thus detoxifying 4-HNE by facilitating the conjugation of 4-HNE to glutathione, forming glutathione-4-hydroxynonenal (GS-HNE). By employing a combination of molecular docking, cellular thermal shift assay, and enzyme activity experiments, we demonstrated that TG exhibited strong binding affinity with AR and inhibited its activity and blocked the conversion of GS-HNE to glutathionyl-1,4-dihydroxynonene (GS-DHN), thereby preventing the formation of protein adducts and inducing severe cellular damage. This study provides novel insights into the anti-inflammatory mechanisms of AR inhibitors and offers potential avenues for developing therapeutic strategies for AR-related pathologies. Our findings suggest that TG, as an AR inhibitor, may hold promise as a therapeutic agent for treating conditions characterized by excessive lipid peroxidation and inflammation. Further investigations are needed to fully explore the clinical potential of TG and evaluate its efficacy in the treatment and management of these complex diseases.
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INDUSTRIAL CROPS AND PRODUCTS
·
2023
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IF 5.9
Yang Yang,Ru Zhao,Hongfei Gao,Zehao Wang,Xinyu Yang,Ming Ruan,Huiyan Gu,Lei Yang,Hao Tian,Changwen Fan,Tingting Liu
In this study, secoiridoids (gentiopicroside, loganic acid, swertiamaroside, and sweroside) were extracted from Gentiana rigescens roots and rhizomes with β-cyclodextrin (β-CD) as the booster and biobased glycine-glycerol deep eutectic solvent (DES) as the extraction solvent and with ultrasound-assisted extraction. The molecular docking performances showed that in DES aqueous solution, β-CD interacts with secoiridoids through noncovalent bonds and hydrogen bonds, and the four secoiridoids studied can be well adapted to the hydrophobic cavity of β-CD. The hydrogen bonds present between secoiridoids and β-CD all reached more than four, and the corresponding binding energies were all lower than − 5.0 kcal/mol. The yields of gentiopicroside, loganic acid, swertiamaroside, and sweroside were 39.82 ± 2.05 mg/g, 6.61 ± 0.34 mg/g, 0.79 ± 0.04 mg/g, and 0.57 ± 0.04 mg/g, respectively, under optimal extraction conditions. The results showed that ultrasound-assisted extraction of secoiridoid compounds in G. rigescens with an aqueous β-CD/DES solution was more effective compared with conventional extraction solvents, which provides a scientific basis for the promising use of β-CD/DES as extraction solvents in natural drugs.
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Open Medicine
·
2023
·Vol. 18
·期 1
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IF 2.1
Haibin Li,Chunyan Zhang,Qiang Liu
Adverse cardiovascular events are associated with vascular calcification (VC) process, where vascular smooth muscle cells (VSMCs) differentiate into osteoblastic phenotype and deposit hydroxyapatite crystals. Microtubule-associated protein kinesin family member 2C (KIF2C) expression is decreased obviously in VSMC during calcification induction. Accordingly, we investigate the role and potential mechanism of KIF2C on VSMC calcification. The effects of β-glycerophosphate (β-GP)/KIF2C/lumican (LUM) on calcification, calcium content, alkaline phosphatase (ALP) activity, calcification-related markers, Tubulin, the ratio of polymerized (Po) to free (Fr) tubulin, as well as levels of LUM, apolipoprotein B (APOB), and KIF2C were assessed by Alizarin red S staining, calcium assay kit, ALP assay kit, Western blot, immunofluorescence, and quantitative real-time PCR. The interplay between LUM and APOB was estimated using co-immunoprecipitation and immunofluorescence. As a result, β-GP promoted calcification of human VMSCs (HVMSCs) and repressed KIF2C expression. KIF2C overexpression reversed the effect of β-GP on HVSMCs. LUM silencing attenuated β-GP-induced promotion on HVSMC calcification and increased KIF2C expression by interacting with APOB. Collectively, LUM silencing can alleviate β-GP-induced VSMC calcification through mitigating the repression of APOB on KIF2C expression.
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