查找使用阿拉丁产品的科研文献

O-GlcNAcylation with ubiquitination stabilizes METTL3 to promoting HMGB1 degradation to inhibit ferroptosis and enhance gemcitabine resistance in pancreatic cancer

Wang Qiuhong,Wei Dong,Li Chunman,Yang Xiawei,Su Kun,Wang Tao,Zou Renchao,Wang Lianmin,Cun Dongyun,Tang Bo,Wu Tao

Section Background Pancreatic cancer is highly lethal, and METTL3 plays a crucial role in the regulation of m6A. However, the therapeutic potential of METTL3 in pancreatic cancer is unclear. Section Methods By means of functional gain and loss experiments, the relationship between METTL3 and gemcitabine resistance as well as ferroptosis was explored. The target genes of METTL3 were screened, and the influence of O-GlcNAcylation on the stability and ubiquitination modification of METTL3 was dissected. Section Results We found that METTL3 is highly expressed in pancreatic cancer and regulates ferroptosis and gemcitabine resistance by promoting the degradation of HMGB1 in an m6A-YTHDF2-dependent manner. O-GlcNAcylation modulates the METTL3’s stability. The interaction between the deubiquitinase EIF3H and METTL3 is influenced by O-GlcNAcylation. Section Conclusion METTL3 affects pancreatic cancer progression by promoting degradation of HMGB1. The O-GlcNAcylation of METTL3 not only stabilizes METTL3 but also augments EIF3H-mediated deubiquitination. These findings uncover potential mechanisms underlying gemcitabine resistance in pancreatic cancer and offer potential therapeutic strategies for the treatment of this disease.

Knockout of bcas3 gene causes neurodevelopment defects in zebrafish

Liu Huihui,Sun Nianyi,Liu Zhenxing,Li Jinze,Zhang Xianqin

Section Background Neurodevelopmental disorders manifest in early childhood and are characterized by cognitive deficits, intellectual disabilities, motor disorders, and social dysfunction. Mutations in BCAS3 gene are associated with syndromic neurodevelopmental disorders in humans, while the detailed pathological mechanism is still unknown. Section Methods CRISPR/Cas9 technology was used to generate a bcas3 knockout zebrafish model. To investigate the effects of bcas3 on development, morphological evaluations were conducted. Locomotor behaviors, including performance in the light-dark test, novel tank test, mirror test, shoaling test, and social test, were assessed through video tracing and quantitative analysis of movement parameters. Transcriptome sequencing analysis was used to identify dysregulated pathways associated with development process. Additionally, Acridine Orange staining was employed to evaluate apoptosis. Western blot and real-time RT-PCR were used to analyze the expression levels of genes. Section Results Bcas3 knockout zebrafish exhibited early larval phenotypes resembling clinical features of patients with BCAS3 mutations, including global delayed development at early embryonic development, microcephaly and reduced body length. Behavior analysis revealed abnormal motor dysfunction, such as social impairment, increased anxiety and heightened aggression. Notably, human BCAS3 rescued the developmental defects and motor disorders in bcas3 knockout larvae. Transcriptomic analysis identified substantial downregulation of genes related to embryonic development and startle response, brain development and neuron migration in bcas3 knockout zebrafish, such as rpl10 , cyfip2 , erbb3b , eya4a , nr2f1b , prkg1b and ackr3b . Additionally, increased apoptosis was observed in bcas3 knockout zebrafish, which was further confirmed by Acridine Orange staining and a decreased Bcl2/Bax ratio in western blot analysis. The increased apoptosis observed in the brain of bcas3 knockout larvae could contribute to the developmental and locomotor deficits. Section Conclusion The bcas3 knockout zebrafish model recapitulates the clinical features observed in patients with BCAS3 mutations. Our results suggest that increased apoptosis may underlie the developmental deficits and motor disorders in these patients. The bcas3 knockout zebrafish model provides a valuable tool to identify dysregulated molecular targets for therapeutic intervention during the early stages of disease progression.

Exploring photocatalytic activity of Sr-doped BiFeO3 multiferroic nanoparticles with structural, magnetic, optical insights

Alhalili Zahrah,Shariq Mohammad,Al-Qasmi Noha,Alqahtani Zahrah,Madkhali O.,Alathlawi Hussain J.,Ali Syed Kashif,Sharma Mukul,Hasan K. F.,Elhelali Thoraya M.

This study used a sol-gel method to prepare BiFeO 3 (BFO) and strontium-doped bismuth ferrite Bi 1-x Sr x FeO 3 (x = 0, 0.01, 0.03, 0.05 and 0.09) (Sr-doped BFO) photocatalysts. The microstructural analysis of the Sr-doped BFO photocatalysts revealed a rhombohedral perovskite structure in the samples. The optical study shows that doping with Sr ions reduced the energy band gap of the photocatalyst from 2.12 eV to 1.97 eV. Moreover, the calculated saturation magnetization (Ms) value was increased after doping with Sr ions, and for Sr (x = 0.05), the doped BFO sample exhibits the highest magnetization value of 0.481 emu/g. It was revealed that the Sr (x = 0.05) doped BFO photocatalyst exhibits 91.2% degradation of methylene blue (MB) dye under visible light radiation. In this study, the formation of trap levels due to the Sr ions, as well as the formation of superoxide anions ( \({O}_{2}^{-}\) ) and OH° radicals played a significant role in the degradation of MB. Graphical

In situ generating poly ionic liquid composite electrolytes supported by mesoporous silica–modified PP separator enabling stable lithium-ion batteries

Gao Chenxiang,Zhang Hengpu,Hu Xiangpan,Ma Xiaoyan

Solid polymer electrolytes (SPEs) are flexible and safer, but inferior ionic conductivity, electrode/electrolyte interface and mechanical strength limit their use for lithium-ion batteries. Herein, we design and prepare a high ionic conductivity, low internal resistance, stable SPE by in situ polymerization of vinylene carbonate and 1-vinyl-3-pentyl cyano imidazole (trifluoromethanesulfonyl) imide in the mesoporous silica-modified polypropylene separators. Mesoporous silica modified polypropylene separators could efficiently adsorb polymer precursor before in-suit polymerization and inhibit lithium dendrite growth during cycling, improving the mechanical strength of SPEs. The poly ionic liquid with many polar groups could effectively promote the dissociation of lithium salts, while its crosslinked network provides Li + transport channels to facilitate the migration of Li + . Meanwhile, in situ polymerization leads to robust electrode/electrolyte interfaces, which reduce the internal resistance and improve the stability of batteries. The optimized SPE (KCE) has a room temperature ionic conductivity of 8.04 × 10 −4 S cm −1 and an electrochemical window of 4.5 V. Assembled Li//KCE//Li cell could run stably at a current density of 0.1 mA cm −2 for 1000 h. Furthermore, the capacity retention rate of LFP//KCE//Li battery after 500 cycles can maintain over 90% and NCM811//KCE//Li battery shows a capacity retention rate of 86.5% after 100 cycles. The LFP//KCE//Li soft pack battery can light an LED bulb and has an open circuit voltage of 2.95 V, showing great potential in the flexibility field. This work provides a workable strategy for improving the performance of solid polymer electrolytes.

A novel polyurethane microcapsule coated with polyacrylamide using D-sorbitol as chain extender for enhanced oil recovery

Wu Yaowei,Jiang Zuming,Wang Yuhao,Jiang Xiancai,Hou Jian,Wei Bei

Polyacrylamide (PAM) serves as an effective thickening agent for oil recovery. However, its viscosity loss during underground transport significantly reduces oil recovery efficiency in reservoir applications. To solve this problem, polyurethane was selected for microencapsulation of PAM through a simple emulsion polymerization method in this work. It is worth mentioning that non-toxic and renewable D-sorbitol was used as a chain extender in the synthesis of polyurethane microcapsules instead of the commonly used diols or diamines obtained from non-renewable petroleum resources, which has not been reported in previous reports. Mechanical stirring was used to simulate the state of polymer subjected to shear during stratigraphic transport. The releasing performance of the microcapsules was characterized by measuring the change of viscosity with time. Meanwhile, we optimized the ratio of D-sorbitol to achieve the longest release time. Experiments showed that D-sorbitol was feasible as a chain extender for the preparation of microcapsules of polyurethane, and the microcapsules prepared at a concentration of 8% D-sorbitol solution had the longest slow-release time of 75 h at 25 °C and 500 rpm. We also prepared samples with BDO as a chain extender for comparison, and the experiments showed that SO8 has a longer slow-release time and can better meet the demand. The prepared microcapsules SO8 exhibited excellent shear resistance and heat resistance, which was attributed to the rigidity and high cross-linking density brought about by D-sorbitol as a hard chain segment of polyurethane. These PAM-containing microcapsules had a promising application in enhancing oil recovery.

Engineering interconnected hierarchical porous carbon frameworks supported FeNi bimetallic sites for enhanced bifunctional oxygen electrocatalysis

Cheng Yi,Li Jiaxin,Lei Ying,Xu Chuanlan,Li Jianying,Si Yujun,Chen Jinlong,Wang Honghui,Han Wenjing

Carbon-supported bimetallic sites show great potential in the field of oxygen reduction electrocatalysis, yet multi-scale structural engineering of carbon supports plays an important role in modulating the catalytic activity. Herein, we ingeniously synthesized an interconnected multi-dimensional porous carbon matrixs supported FeNi bimetallic catalyst (denoted by FeNi-NC/CNT) through pyrolyzing ZIF-8 precursors mixed with polystyrene (PS) microspheres as a pore-forming agent and carbon nanotubes. The optimized FeNi-NC/CNT catalyst exhibited exceptional bifunctional catalytic activity with high half-wave potential E 1/2  = 0.87 V in the oxygen reduction reaction (ORR) process and high activity in the oxygen evolution reaction (OER) with E j=10  = 1.60 V, leading to an ultralow potential difference (730 mV) that is much lower than that of control sample (843 mV) without PS perforation. In practical application, the FeNi-NC/CNT based zinc-air battery achieved a peak power density of 148.6 mW cm −2 and a steady operation time exceeding 150 h, with a low potential difference of only 950 mV. The enhanced bifunctional catalytic activity can be attributed to multi-dimensional frameworks composed of hierarchical porous carbons and conductive CNTs, provide adequate exposure to active sites and mass/electron transfer pathway, and the tuned interaction of FeNi that contributes to equilibrating the intrinsic ORR/OER bifunctional catalytic activity. Graphical abstract

Fabrication of a novel bone adhesive (crosslinked phytic acid-gelatin coordinated with magnesium phosphate and calcined dolomite, and montmorillonite) for enhancing adhesion strength and biocompatibility

Hu Man-Na,Qu Xue-Jing,Chen Xiao-Lan,He Dan,Wang Guo-Ya,Liu Jia-Hui,Roy Tania,Kurniawan Alfin,Zhou Chun-Hui

Bone adhesives are known as a promising fracture treatment material because they can quickly heal broken bones. However, the existing bone adhesives have the disadvantages of weak binding ability to bone tissue, non-absorption, or difficulty in curing under wet conditions, which limits their wide application in the field of bone tissue engineering. In this study, the raw material of magnesium phosphate bone adhesive was modified, and the composite material of magnesium phosphate bone repair was prepared by the method of solid–liquid blending crosslinking. Mineral-organic composite, a new type of bone adhesive was prepared using calcined dolomite and montmorillonite as mineral components, phytic acid and gelatin as organic components. The compressive strength, porosity and bonding strength of the magnesium phosphate-based bone adhesive were analyzed by Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy. When the dosage of dolomite is 8 wt% and the concentration of gelatin is 9 wt%, the adhesion strength of the bone adhesive is 2.04 MPa after 168 h placement. And the compressive strength of the bone adhesive was 6.66 MPa after 168 h placement. After the prepared bone adhesives were immersed in SBF solution for 14 and 21 days, EDS analysis showed that the accumulated material was bone-like hydroxyapatite, indicating that the prepared bone adhesives had good osteogenic activity. In addition, it was also found that the bone adhesive had fluid absorption ability and no cytotoxicity. So, conclusively it can be stated that such newly synthesized bone adhesive has significant medical potential.

Multifunctional lanthanide metal-organic framework based ratiometric fluorescence visual detection platform for alkaline phosphatase activity

Xiong Li,Yu Long,Li Shuo,Feng Lixiang,Xiao Yuxiu

A turn-on/off ratiometric fluorescence detection platform based on multifunctional lanthanide metal-organic framework (Ln-MOF) and an enzymatic cascade reaction is proposed for alkaline phosphatase (ALP) activity assay. L-phosphotyrosine is hydrolyzed to levodopa (L-dopa) by two steps of enzymatic reaction. L-dopa further reacts with naphthoresorcinol to produce carboxyazamonardine with strong emission at 490 nm. In this process, multifunctional Ln-MOF (Cu@Eu-BTC, BTC is the 1,3,5-benzenetricarboxylic acid) acts not only as a nanozyme to catalyze the fluorogenic reaction between L-dopa and naphthoresorcinol but also as a fluorescence internal standard. The emission of Cu@Eu-BTC at 620 nm is quenched by phosphate anions, and the dual-response ratiometric fluorescence (F 490 /F 620 ) can be achieved. A good linear relationship was obtained between Δ(F 490 /F 620 ) and ALP activity in the range 0.3–24 U L −1 with the detection limit of 0.02 U L −1 . In addition, a portable assay tube was designed for visual and point-of-care testing of ALP activity by color variation (ratiometric chromaticity). Both the ratiometric fluorescence detection and the visual detection methods were successfully applied to monitor ALP activity in human serum samples with recovery between 95.5%–109.0% and 94.0%–110.1%, and relative standard deviation less than 8.1% and 9.5%, respectively. As far as we know, this is the first report of ALP activity assay assisted by multifunctional Ln-MOF. Graphical abstract

Contrasting effects of three aging processes on arsenic immobilization in red versus black soils amended by cerium-manganese modified biochar: the unique role of freeze–thaw cycling in governing arsenic fate at micro/nano interfaces

Lyu Peng,Huang Xiaoya,Li Lianfang,Jiao Yan

The aging mechanisms of modified biochar for arsenic (As) immobilization at micro/nano-interfacial scales in diverse soils remain poorly understood. Herein, we employed three aging treatments, including natural aging (NA), freeze–thaw cycles (FT), and dry–wet alternation (DW), to simulate the aging behavior of cerium-manganese modified biochar (CMBC) in two As-contaminated field soils. Results indicated that CMBC amendment significantly reduced soil pH by 7.5–16.7%, while simultaneously increasing dissolved organic carbon contents by 10–45%, available phosphorus levels by 11–43%, and the activities of four soil enzymes by 30–320% in comparison to unamended soils. These improvements proved to be most effective under FT-aging, followed by DW-aging and NA-aging. FT-aging also led to the most pronounced reduction in water-soluble As concentrations ranging from 94 to 99%, as well as a decrease in As mobilization coefficients of 38% to 59% in CMBC-amended soils when compared to DW-aging and NA-aging. The superior As immobilization under FT-aging can be attributed to adhesion mediated by Ce–Si crystal nano-bridge between soil microparticles and CMBC matrix, whereas such adhesion was not observed in NA/DW-aged samples. This unique interfacial configuration promoted Ca/Fe-oxide intercalation and amorphous Ce-oxides formation within CMBC, which facilitated the development of As–Fe/Ce crystalline phases. Meanwhile, the synergistic enrichment of metallic and oxygen-containing groups on FT-aged CMBC surface induced the formation of stable As–Ce/Fe–O species and triggered dual redox transformations: (1) Ce/Mn reduction drove bulk As(III) oxidation to As(V), and (2) Fe(0) oxidation mediated partial reduction of As(V)/As(III) to inert As(0). Notably, CMBC-amended red soil exhibited preferential As immobilization during aging due to the tighter adhesion between nano-CMBC and soil colloids. This enhanced adhesion strengthened the bonding of Ce/Fe-oxides with As and intensified the oxidation of As(III) to As(V) through increased Ce/Mn reduction. This study provides innovative microscale mechanistic insights into the aging behavior of modified biochar for remediating diverse soils contaminated with potentially toxic elements. Graphical

An LCA-assisted hierarchical design of radiative cooling coating for full life-cycle CO2 reduction

Cao Ningning,Chi Haowen,Chen Yuxin,Zhu Chen,Liu Tianji,Li Jiabao,Ma Yue,Zhu Bin,Wang Xueyang,Zhao Ke,Wang Zhenyu,Li Wei,Zhao Liang,Guan Dabo,Chen Jun,Zhu Jia

Reducing CO 2 emissions throughout a product’s life cycle, mainly encompassing four stages of raw materials extraction, processing, service, and end of life, is essential for carbon neutrality. Passive daytime radiative cooling coatings offer CO 2 reduction benefits, but primarily only during service stage. Life cycle assessment demonstrates maximized full life-cycle CO 2 reduction requires innovations in both raw materials extraction and service periods. Here, we develop a full life‑cycle carbon‑negative passive daytime radiative cooling coating, achieving 0.571 ~ 13.709 tons of CO 2 -equivalent reduction per ton over its lifespan across all climate zones compared to the commercial reflective cooling baseline, equivalent to planting 32 ~ 762 trees annually. The key to this is sodium dodecyl sulfate-induced hydromagnesite fillers, produced via scalable CO 2 mineral sequestration, which mitigate emissions in raw materials extraction stage. Moreover, the durable polyvinylidene fluoride resin envelops the above fillers with a hierarchical spherical-like structure, providing high optical characteristics and service stability, thus achieving service-stage emissions reduction. This work converges life cycle assessment, material design, and CO 2 mitigation technology, building a powerful platform for carbon-negative radiative cooling coatings and relevant materials, while also inspiring more advancement in sustainable technologies for resilient urban futures.

A universal strategy towards self-healing materials via dynamic interfacial liquid metal coordination

Li Zhiwei,Zhang Yue,Liu Songlin,Lan Jingyu,Peng Yan,Zhang Jiuyang

Self-healing polymer materials, capable of autonomously repairing physical damage, have been broadly applied in modern technologies. In various self-healing systems, metal–ligand coordination bonds have been extensively utilized for their advantages of rich metal–ligand species and functionalities. However, common metal-ligand coordination either has excessively stable bond strengths or is too weak to construct self-healing materials. This work introduces coordination metals into liquid metals (LMs) to form multi-component LMs (mLMs), which creatively leverage the inherent fluidity of mLMs to convert common metal-ligand coordination (e.g., silver-sulfur and zinc/copper-imidazole systems) into reversible interfacial coordination. Such dynamic coordination successfully offers the fantastic self-healing efficiency over 90% for general polymers. Considering the ultra-high thermal conductivity of mLMs, self-healable thermal interface materials (TIMs) are obtained, which successfully address the long-standing challenge of the irreversible damage in long-term used TIMs. The self-healable TIMs can lower the peak temperature of the central processing unit (CPU) by 20 o C under extreme conditions for long time (accumulated 16 hours thermal shock, −10 o C to 100 o C). This work provides a universal strategy for self-healing materials and greatly broadens the investigations of self-healing, coordination chemistry, liquid metal science, soft electronics, and thermal management materials.

Comparison of Freeze-drying and Gradient Dehydration Treatment on X-ray Imaging for Three-dimensional Reconstruction of Early Human Embryonic Brain Samples

Yao Yangqianbo,Hao Wenjie,Wu Shengju,He Qizhi,Xiao Tiqiao,Zhang Zhijun