ENVIRONMENTAL POLLUTION
·
2023
·
IF 8.9
Xiaojing Li,Qian Wu,Dan Chen,Yichen Bai,Yuhong Yang,Shiwen Xu
Cadmium (Cd) is a major contaminant in natural environments and exerts adverse effects on aquatic biota at low concentrations. Gill is as vital respiratory organ and may cause pollutants to enter fish during gas exchange. Baicalein (BAI), as a kind of flavonoids, possess antioxidant properties through inactivating free radicals. To confirm the potential effects and approaches of BAI addition in maintaining the gill stability, 90 common carps ( Cyprinus carpio L.) were selected and randomly divided into water environment exposure group (0.22 mg/L Cd) and/or feed added with 0.10 g/kg BAI for 30 days. The analysis of ion content in serum showed that Cd exposure disturbed ion homeostasis , and BAI could reduce serum Cd concentration. The histopathological results of gills showed that Cd exposure caused gill tissue lesions and structural damage, and BAI feeding effectively alleviated this damage. In addition, BAI could enhance antioxidant activity and activate Nrf2/HO-1 axis, thereby reducing oxidative stress and endoplasmic reticulum (ER) stress. Moreover, BAI lightened cytokine imbalance, inflammatory response, and necroptosis. Overall, the results indicated that BAI feeding could maintain gill homeostasis against Cd poisoning via the ROS/ER stress signaling. This trial revealed the properties of BAI resistance to metal Cd in aquaculture and partially elucidated its mechanism.
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高影响因子
FOOD HYDROCOLLOIDS
·
2023
·
IF 10.7
Yamei Wu,Yanyan Wu,Huan Xiang,Shengjun Chen,Yongqiang Zhao,Qiuxing Cai,Yueqi Wang
In this study, we aimed to investigate the influence of lysine-assisted ultrasonic treatment on the conformation, and interfacial and emulsification properties of sea bass myofibrillar proteins at the oil–water interface. It was observed that the modification treatment with lysine (1 g/L) combined with low-intensity ultrasound (200 W) enhanced the interfacial pressure and penetration rate. This enhancement could be attributed to the significant structural unfolding of myofibrillar proteins located at the oil–water interface (increase in β-sheets and reduction in α-helices), which exposed the hydrophobic groups. The rate of interfacial protein adsorption subsequently increased, owing to the strengthening of protein–protein and protein–oil phase interactions. Additionally, the interfacial swelling rheology experiment showed an enhancement in the ordered structure of interfacial proteins, aiding in the development of a protein gel network structure. Consequently, the elastic modulus of the oil–water interface was larger, and the droplet interfacial structure was tight, which facilitated uniform distribution and improved the emulsification properties of the droplets, ultimately enhancing the ability of the emulsions to be stored at room temperature.
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MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Jiabo Cheng,Mengfei Tian,Zarmina Gul,Chunyu Liang,Bin Qiao,Yuan Gao,Chunjian Zhao,Chunying Li
In this study, a functionalized surface active ionic liquid (SAIL) was designed and prepared, which exhibits pH-responsive behavior and high yield for 6 target flavonoids from the leaves of Vitex negundo L.. Firstly, a series of functionalized SAIL [N-C n , N'–CO 2 H-Im]Br (n = 10, 12, 14, 16) were prepared and used for extraction of the target components, [N-C 14 , N'–CO 2 H-Im]Br showed the highest yield. Secondly, optimized extraction conditions obtained by Plackett-Burman design (PBD) and response surface methodology (RSM) were concentration of 45.5 mM, liquid–solid ratio of 11.6 (mL/g), extraction time of 38.5 min, extraction temperature of 40 °C, and ultrasonic power of 400 W. The yield obtained by [N-C 14 , N'–CO 2 H-Im]Br of vitexin, isovitexin, hyperoside, luteolin, quercetin, apigenin is 2.04, 1.81, 1.24, 1.51, 2.47 and 1.21 mg/g, respectively, which is 1.11–1.56 folds higher than non-functionalized SAIL ([C 14 min]Br) and 1.50–5.36 folds higher than traditional solvents. More importantly, the solvent system can realize the in-situ separation of the target compound by adjusting pH. When pH is changed from 6.1 to 4.9, the system is divided into two phases, and the target compounds were separated to the upper phase with a high recovery rate. Finally, molecular simulation was used to reveal the extraction mechanism of functionalized SAIL on target compounds. The results showed that the target flavonoids were compatibilized to the palisade region of micelles, and the weak interaction between the target flavonoids and functionalized SAIL was mainly van der Waals force.
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JOURNAL OF ETHNOPHARMACOLOGY
·
2023
·
IF 5.4
Danilo de Souza Costa,Carla Monteiro Leal,Rayssa A. Cajas,Matheus Coutinho Gazolla,Lívia Mara Silva,Lara Soares Aleixo de Carvalho,Bruna L. Lemes,Renato Oliveira de Moura,Juliana de Almeida,Josué de Moraes,Ademar A. da Silva Filho
Ethnopharmacological relevance Roots of Pothomorphe umbellata (L.) Miq. are used in traditional medicine of Africa and South America for the treatment of malaria and helminthiasis . However, neither P. umbellata nor its isolated compounds have been evaluated against Schistosoma species. Aims of this study To investigate the antischistosomal effects of P. umbellata root extracts and the isolated compound 4-nerolidylcatechol (4-NC) against Schistosoma mansoni ex vivo and in murine models of schistosomiasis. Materials and methods The crude hydroalcoholic (PuE) and hexane (PuH) extracts of P. umbellata roots were prepared and initially submitted to an ex vivo phenotypic screening against adult S. mansoni . PuH was analyzed by HPLC-DAD, characterized by UHPLC-HRMS/MS, and submitted to chromatographic fractionation, leading to the isolation of 4-NC. The anthelmintic properties of 4-NC were assayed ex vivo against adult schistosomes and in murine models of schistosomiasis for both patent and prepatent S. mansoni infections. Praziquantel (PZQ) was used as a reference compound. Results PuE (EC 50 : 18.7 μg/mL) and PuH (EC 50 : 9.2 μg/mL) kill adult schistosomes ex vivo . The UHPLC–HRMS/MS analysis of PuH, the most active extract, revealed the presence of 4-NC, peltatol A, and peltatol B or C. After isolation from PuH, 4-NC presented remarkable in vitro schistosomicidal activity with EC 50 of 2.9 μM (0.91 μg/mL) and a selectivity index higher than 68 against Vero mammalian cells, without affecting viability of nematode Caenorhabditis elegans . In patent S. mansoni infection, the oral treatment with 4-NC decreased worm burden and egg production in 52.1% and 52.3%, respectively, also reducing splenomegaly and hepatomegaly . 4-NC, unlike PZQ, showed in vivo efficacy against juvenile S. mansoni , decreasing worm burden in 52.4%. Conclusions This study demonstrates that P. umbellata roots possess antischistosomal activity, giving support for the medicinal use of this plant against parasites. 4-NC was identified from P. umbellata roots as one of the effective in vitro and in vivo antischistosomal compound and as a potential lead for the development of novel anthelmintics.
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高影响因子
AQUACULTURE NUTRITION
·
2023
·
IF 3.5
Jianxin Zhang,Mengyuan Huang,Junchang Feng,Yongyan Chen,Meng Li,Xiaolin Meng,Xulu Chang
This study is aimed at exploring the effects of Bacillus coagulans NRS 609 on the growth performance, intestinal health, antioxidant activity, and immune response in common carp (Cyprinus carpio L.) by colonizing the intestinal tract with the beneficial strain. Gavage B. coagulans with GFP-tag at 109 CFU/mL into common carp for 7 days. Results indicated that B. coagulans were maintained in the intestine for at least 14 days. Four groups fed B. coagulans at different levels of inclusion, including 0 (CK), (A), (B), and (C) CFU/g of feed for 8 weeks. Experimental groups showed significantly better growth performance than the control (). The treatment groups had higher digestive enzyme activity, villus height, and muscle thickness than the control (). Supplementation with B. coagulans also altered the composition of the intestinal flora. In addition, an enhancement of the antioxidant enzyme and a reduction of malondialdehyde in the liver were observed with the addition of B. coagulans (). Moreover, the cytokines of IL-1β and IL-12, IL-10, and TGF-β, significant upregulation in experimental groups (). Based on the results, the optimal level of B. coagulans was 107 CFU/g in diets for common carp.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Qianni Chen,Jiaqi Fan,Lianzhu Lin,Mouming Zhao
The combination of polysaccharides is an effective way to develop prebiotics with stable performance during processing and digestion for human wellness. However, there is little information on optimal screening and complementary regulation of compound polysaccharides. This study aimed to optimally select a combination of Lycium barbarum L. polysaccharide (LBP) and Laminaria japonica polysaccharide (LJP) as a highly efficient prebiotic to regulate the gut probiotics and their metabolites. Two LBPs characterized as rhamnogalacturonan I enriched pectins and two LJPs characterized as fucoidans were obtained by enzyme-assisted acid extraction at moderate and dramatic temperatures and combined in pairs to obtain 4 groups containing 4 proportional combinations. All combinations showed better prebiotic effects than individual LJP. The combination of LBP and LJP extracted at 50 °C at a ratio of 4:1 exhibited the strongest prebiotic effect. The optimal compound polysaccharide achieved superior effect and complementary function via LBP-targeted proliferation of Bifidobacterium , Lactobacillus , and Bacteroides and production of SCFAs and non-SCFA health-associated metabolites, LJP-targeted accumulation of butyrate-producing bacteria and corresponding metabolites, as well as synergistic effect of LJP and LBP at exact proportion. Our study provided theoretical and methodological guidance for optimal screening of compound polysaccharides as new prebiotics.
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高影响因子
ANALYST
·
2023
·Vol. 148
·期 8
·1877-1886
·
IF 4.2
Jiahe Huang,Zhengjin Zhou,Chunhong Zhang,Chao Wang,Yanli Zhou,Lijia Liu,Junqing Li,Toshifumi Satoh,Yoshio Okamoto
A novel one-handed helical copoly(phenylacetylene) (CPA) bearing L-proline tripeptide pendants and a few triethoxysilyl residues was synthesized and hybridized into SiO2 porous microspheres (PMSs) during microsphere growth through the hydrolytic polycondensation of ethoxysilyl groups. Nuclear magnetic resonance and Fourier transform infrared spectroscopy results verified the successful preparation of CPA and its hybrid product using SiO2 PMSs. The chiral recognition ability of the resulting CPA with a hybridized-type chiral stationary phase (HCSP) for high-performance liquid chromatography (HPLC) was investigated, revealing its high recognition ability for selected racemates. Moreover, the HCSP showed good solvent tolerability, thus broadening the selection of suitable eluents. The separation effect of the HCSP for the racemate N,N-diphenylcyclohexane-1,2-dicarboxamide (7) improved significantly after introducing CHCl3 in the eluent, resulting in separation factors equivalent or superior to common commercially available polysaccharide-based chiral stationary phases. The proposed preparation strategy provides a new and valuable method for obtaining poly(phenylacetylene)-based HCSPs suitable for a wide range of applications and eluent conditions.
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高影响因子
ACS Biomaterials Science & Engineering
·
2019
·
IF 4.511
Linlong Li,Xincui Shi,Zongliang Wang,Min Guo,Yu Wang,Zixue Jiao,Peibiao Zhang
Three-dimensional (3D) composite porous scaffolds containing hydroxyapatite (HA) and polymer matrix showed wide applications in bone repair because of their improved biocompatibility, bioactivity, and mechanical properties in our previous studies. In this work, mesoporous hydroxyapatite (MHA) surface-modified by poly(γ-benzyl-l-glutamate) (PBLG) with different amounts (from 11 to 50 wt %) was synthesized by the in situ ring opening polymerization of γ-benzyl-l-glutamate N-carboxy anhydride (BLG-NCA) and then PBLG-g-MHA/PLGA composite films were prepared to illustrate the biological performance of the composites. Furthermore, porous scaffolds of PBLG-g-MHA/PLGA were fabricated through modified solvent casting/particulate leaching (SC/PL) method to demonstrate the ability of in vivo bone defect repair. In vitro cytological assay indicated that enhanced cell expansion on PBLG-g-MHA/PLGA with 11 wt % PBLG amounts and improved osteogenic differentiation on the composites with 33 and 50 wt % PBLG amounts were achieved. And the porous scaffolds exhibited high porosity and interconnected pores. Results of the in vivo rabbit radius defect repair indicated that rapid mineralization and new bone formation could be observed on the composites with 22 and 33 wt % PBLG. This study revealed that PBLG-g-MHA/PLGA composites might have potential applications in clinical bone repair.
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JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2019
·
IF 2.298
Yuwen Fan,Wenjun Zhu,Yonghong Hu,Wenge Yang,Qiang Xu,Xinxin Liu,Bin Heng
In the study, the solubility of l-serine were measured in eight common pure solvents and binary mixed solvents (acetonitrile + water, isopropanol + water, methanol + water, and acetone + water) from 278.15 to 333.15 K under atmospheric pressure by gravimetric method (acetone was measured from 278.15 to 328.15 K). The solubility of l-serine increases with increasing temperature, and the order in eight common pure solvents is water > acetonitrile > methanol > acetone > ethyl acetate > ethanol > n-butanol > isopropanol. In binary mixed solvents, the solubility of l-serine is dependent on temperature and solvent composition. Also, the solubility of l-serine increases with increasing temperature, and it decreases as the concentration of acetonitrile, isopropanol, methanol, or acetone increases at a constant temperature. The experimental data in eight pure solvents were fitted by the modified Apelblat model, Buchowski–Ksiazaczak λh model. The modified Apelblat equation, combined nearly ideal binary solvent/Redlich–Kister (CNIBS/R–K) model, and Jouyban–Acree model were used to correlate the experimental data in four binary solvents. Among the four models, the modified Apelblat model is more suitable with a higher accuracy than the other three models. The experimental data can be used to optimize the industrial manufacturing process of l-serine.
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高影响因子
Foods
·
2019
·Vol. 8
·期 6
·205
·
IF 3.011
Wei Zhang,Xianrui Liang
In this study, natural deep eutectic solvents (NADESs) were used as both the extraction and dilution matrix in static headspace gas chromatography-mass spectrometry (SHS-GC-MS) for the analysis of volatile components inIpomoea cairica(L).Sweet(ICS) leaves. Six NADESs were prepared and the NADESs composed of choline chloride and glucose with a 1:1 molar ratio containing 15% water were preferred due to the better peak responses. A total of 77 volatiles inICSleaves were detected and tentatively identified by mass spectral matching with the US National Institute of Standards and Technology (NIST, 2014) Mass Spectral Library and the retention index-assisted qualitative method. These 77 volatile components were mainly terpenoids, aromatics, and aliphatics. Among them, β-elemene, β-caryophyllene, α-humulene, and 2, 4-di-tert-butylphenol were found to be the main components. This investigation verified that the use of NADESs is an efficient green extraction and dilution matrix of the SHS-GC-MS method for direct volatile component analysis of plant materials without extra extraction work.Keywords:Ipomoea cairica(L.)Sweetleaves;natural deep eutectic solvents;static headspace gas chromatography-mass spectrometry;volatile components
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Polymers
·
2019
·Vol. 11
·期 7
·1123
·
IF 3.164
Hui Liang,Yun Zhao,Jinjun Yang,Xiao Li,Xiaoxian Yang,Yesudass Sasikumar,Zhiyu Zhou,Minfang Chen
The present work focuses on the preparation of poly(l–lactide)–magnesium oxide whiskers (PLLA–MgO) composites by the in-situ polymerization method for bone repair and implant. PLLA–MgO composites were evaluated using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), differential scanning calorimetry (DSC), scanning electron microscopy (SEM) and solid-state13C and1H nuclear magnetic resonance spectroscopy (NMR). It was found that the whiskers were uniformly dispersed in the PLLA matrix through the interfacial interaction bonding between PLLA and MgO; thereby, the MgO whisker was found to be well-distributed in the PLLA matrix, and biocomposites with excellent interface bonding were produced. Notably, the MgO whisker has an effect on the crystallization behavior and mechanical properties; moreover, the in vivo degradation of PLLA–MgO composites could also be adjusted by MgO. These results show that the whisker content of 0.5 wt % and 1.0 wt % exhibited a prominent nucleation effect for the PLLA matrix, and specifically 1.0 wt % MgO was found to benefit the enhanced mechanical properties greatly. In addition, the improvement of the degrading process of the composite illustrated that the MgO whisker can effectively regulate the degradation of the PLLA matrix as well as raise its bioactivity. Hence, these results demonstrated the promising application of PLLA–MgO composite to serve as a biomedical material for bone-related repair.Keywords:PLLA–MgO whiskers composites;in-situ polymerization;bone repair and fixation;mechanical properties and crystallization behavior;regulating in-vivo degradation
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2018
·
IF 3.141
Huijiao Yan,Long Chen,Aiying Bai,Hengqiang Zhao,Meng Kong,Li Cui
Ginkgolic acids (GAs), bioactive compounds found in Ginkgo biloba L., have received much attention in pharmacological research. Separation of individual GAs from their mixtures is, however, difficult. Herein, an efficient method for the preparative separation of five major GAs from the sarcotesta of Ginkgo biloba L. was developed using β-cyclodextrin (β-CD) clathration coupled with pH-zone-refining countercurrent chromatography (PZRCCC) and recycling CCC. Clathration by β-CD was employed to enhance the hydrophilicity of the GAs. Separation of β-CD-GA clathrates was achieved by PZRCCC using a two-phase solvent system composed of n-heptane–ethyl acetate–methanol–water (2:1:1.5:1, v/v). The upper phase was acidified with 40 mM HCl (stationary phase), and the lower phase was made alkaline with 5 mM triethylamine. Three GAs were obtained with purities over 98% together with two mixtures. The mixtures were separated in recycling CCC mode using n-heptane–ethyl acetate–methanol–acetic acid (5:4:1:1, v/v) as the solvent system. Five GAs, including C13:0, C15:0, C15:1, C17:1, and C17:2, were successfully separated with purities over 98%, and their structures were confirmed by comparison with a mixture of GA standard samples coupled with ESI-MS data.
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危险品化学品经营许可证（带存储）