CARBOHYDRATE POLYMERS
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2025
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IF 12.5
Qijun Yu,Jinshui Yang,Liang Liu,Yaru Huang,Entao Wang,Dongmei Li,Hongli Yuan
Enzyme immobilization technology holds significant value in biocatalysis. Carbohydrate-binding modules (CBMs), with their specific binding to natural polysaccharides, offer a highly promising immobilization method. In the present study, the binding ability with their natural substrates and heterologous expression levels of four CBMs using fluorescent protein tagging were studied, revealing that CBM2r presented the highest immobilization efficiency and expression level. Using the Design of Experiments (DOE), the immobilization conditions for mCherry-CBM2r were optimized, achieving a protein loading of 2.45 wt% on Avicel under optimal conditions: a solid-liquid ratio of 1:30, NaCl concentration of 108 mM, protein concentration of 6 mg/mL, and incubation time of 120 min. Subsequently, CBM2r gene was fused with chitosanase gene from Bacillus subtilis (BsCsn) and expressed in Escherichia coli for establishing a novel one-step immobilization of fusion enzymes mediated by CBM2r on microcrystalline cellulose. The immobilized CBM2r-BsCsn-Avicel was used for batch hydrolysis of high-concentration chitosan to produce chito-oligosaccharides, with the enzyme retaining 96 % substrate degradation efficiency over seven cycles and achieving a space-time yield of 232.8 kg/m 3 /h. This study provides a simple, cost-effective, environment friendly, and competitive biocatalytic immobilization strategy.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Mengxia Wang,Xiaran Miao,Feng Guo,Zhiqing Deng,Fenggang Bian,Tiqiao Xiao,Chunhai Chen
The use of renewable bioresources and their nanoforms in developing edible coating materials is considered a promising approach for preserving food freshness. Herein, cellulose nanofibers (CNF) with different morphologies were combined with gelatin to prepare composite preservation film following by brushing over the surface of cherry tomatoes as an edible coating. The gelatin-based composite film containing 0.3 % CNF20 (GC2–0.3) exhibited the lowest water vapor permeability (WVP, 1.97 × 10 −4 barrer), lower oxygen permeability (OP, 2.54 × 10 −2 barrer), higher transparency (Tr = 85.28 %) and excellent mechanical properties (σ = 47.45 MPa, E = 1.84 GPa). When coated on cherry tomatoes, it maintained good luster and freshness, significantly reducing the water loss of cherry tomatoes. The weight loss was only 16 % after 14 days of storage at 25 °C and 30 % humidity, compared to >30 % for the uncoated cherry tomatoes. This work provides a viable strategy for developing sustainable, green fresh-keeping materials that can prolong the storage time of the putrescible food.
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CHEMICAL ENGINEERING SCIENCE
·
2024
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IF 4.1
Qian Li,Ke Qiu,Changhai Li,Huan Li,Mengqing Zhang,Haijun Liu
Firefighting foam stability is a critical aspect in determining fire extinguishing efficiency. The stability and rheological properties of environmental firefighting foams are investigated in this work. The results show that increasing NaCMC polymer concentration decreases the equilibrium surface tension of CTAB/501W surfactants, while enhancing the viscosity and conductivity. And the yield strain and yield stress of the foam improve. However, when the NaCMC concentration is enhanced to 0.8 wt.%, the NaCMC concentration has less effect on the change in rheological characteristics. Slight NaCMC concentration addition enhances the foaming ability of CTAB/501W surfactant solutions, but further addition of NaCMC concentration decreases its foaming ability. Increasing NaCMC cocentration reduces the height decay of the foam produced by CTAB/501W surfactant solutions, retarding the bubble coarsening and liquid drainage process, and leading to foam stability enahncement, where the stability turning point occurs when NaCMC concentration is 0.5 wt.%. This is mainly attributed to the interaction between NaCMC polymer and CTAB molecule, and the increased viscosity.
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Sustainable Materials and Technologies
·
2025
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IF 9.2
Yan Xu,Qingyu Wu,Dicong Yang,Biwei Qiu,Xiaoliang Mo,Bo You,Limin Wu
It is a huge challenge to design multifunctional superhydrophilic coatings by bio-based raw materials and low carbon emissions in full life cycle. As a green, hydrophilic, low-cost material, cellulose has irreplaceable advantages in the preparation of sustainable superhydrophilic coatings. Here, a novel and large-scale transparent saxifrages-biomimetic superhydrophilic cellulose-based coating (SES) was fabricated by one-step method through blending semi-wet maleic anhydride esterified cellulose (EC) modified cellulose with waterborne styrene-acrylate latex (SAL) and sodium dodecyl sulfate (SDS). The EC was chosen as fillers because of its smallest particle size and the best dispersion of cellulose in waterborne latex. With the addition of SDS, affected by polarity, the hydrophilic head group orientally arranges the cellulose to form a saxifrage-like structure on the surface of coating. The obtained superhydrophilic coating with a static water contact angle of 0° and a waterborne oil contact angle of 157.1°, and the coating remained in a superhydrophilic state after 600 times of wear resistance cycle test. The SES coatings have excellent performance in anti-fogging, biological anti-fouling and oil-water separation, this is due to the synergy effects of the micro-nano scale roughness, high surface energy and hydrophilic group of coating film. The life cycle assessment (LCA) analysis of saxifrages-biomimetic SES superhydrophilic coating proved that the cellulose-based coatings were low-cost, easily prepared, and non-pollution, reducing carbon emission.
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CARBOHYDRATE POLYMERS
·
2024
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IF 10.7
Xiaokai Jing,Sufeng Zhang,Fengjiao Zhang,Congcong Chi,Shuyuan Cui,Hao Ding,Jinrui Li
Due to the unsatisfactory mechanical properties of natural polymer-based conductive hydrogels , their applications are limited. Shaanxi Biangbiang noodles can be toughened by applying external mechanical forces through stretching and beating movements; this process provides inspiration for the preparation of high-strength hydrogels. In this paper, we propose a strategy for the preparation of ultrastrong and ultratough conductive hydrogels by directional prestretching and solvent exchange. Neatly arranged fiber bundles containing many intermolecular hydrogen bonds and metal ion coordination bonds are successfully constructed inside the prepared hydrogels. The hydrogel has exceptional mechanical properties, with a fracture stress exceeding 50 MPa, fracture strain approaching 105 %, fracture toughness exceeding 30 MJ m −3 , and high conductivity reaching 11.738 ± 0.06 mS m −1 . Impressively, the hydrogel can maintain its high mechanical properties after being frozen at an ultralow temperature of −80 °C for 7 days. Compared with other tough hydrogels, natural tendons and synthetic rubbers , the hydrogel exhibits excellent mechanical properties. The cellulose-based conductive hydrogel prepared in this study can be applied to robotic soft tissues (such as the Achilles tendon) that require high strength and are operated in extreme environments.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Chenguang Jiang,Yuchen Chao,Wenyuan Xie,Defeng Wu
Network optimization is vital for the polysaccharide based hydrogels with multiple crosslinks. In this study, we developed a ‘two-step’ strategy to activate synergistic effect of chemical and physical crosslinks using a poly (vinyl alcohol) (PVA)/bacterial cellulose (BC) hydrogel as a template. The BC nanofibers, on the one hand, acted as nucleating agents, participating in the crystallization of PVA, and on the other hand, were also involved in the formation of boronic ester bond, anchored with the PVA chains via chemical bonding. Therefore, the existence of BC nanofibers, as ‘bridge’, linked the crystalline regions and amorphous parts of PVA together, associating the two characteristic crosslinks, which was conducive to load transfer. The mechanical properties of resultant hydrogels, including the tensile elongation and strength, as well as fracture toughness, were significantly improved. Moreover, the dually cross-linked hydrogels possessed ionic conductivity, which was sensitive to the tensile deformation and environmental temperature. This study clarifies a unique role of BC nanofibers in hydrogels, and proposes an effective approach to construct multiple networks in the nanocellulose reinforced PVA hydrogels.
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Green Energy & Environment
·
2025
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IF 14.6
Kaile Li,Jiahui Hu,Zhiqiang Xu,Shijie Yu,Qinghai Li,Yanguo Zhang,Hui Zhou
Hydrothermal treatment of cellulose is a promising green route for bioenergy and biochemical production, yet requiring investigations of the mechanisms. In this study, the effects of cellulose crystallinity and decoupled temperature and pressure conditions on cellulose conversion and product distribution were investigated. Microcrystalline cellulose was ball-milled for varying durations, leading to a reduction in crystallinity, with 4 h of milling sufficient to achieve near-complete amorphization. Unlike concurrent recrystallization and hydrolysis observed under autogenous pressure, decoupled conditions significantly accelerated hydrolysis of cellulose. Notably, lower crystallinity cellulose exhibited significant improvements in glucose and 5-HMF yields, with 4-hour ball milling showing optimal performance among all samples. Furthermore, carbon sub-micron spheres were largely produced, which were confirmed via PTFE encapsulation experiments to primarily consist of secondary char deriving from re-polymerization and condensation reactions of the liquid phase. Overall, this study demonstrates that lower crystallinity not only facilitates hydrolysis but also accelerates the carbonization processes under decoupled pressure conditions, highlighting its potential for efficient biomass conversion into valuable products.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2024
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IF 8.1
Jinyu Liu,Zikang Qin,Linlin Yang,Jing Wei,Chao Feng,Bo Tang,Wenju Jiang,Lin Yang,Lu Yao,Junfeng Zheng,Zhongde Dai
Membranes based on two-dimensional (2D) materials often show great separation efficiency and therefore have great potential in the treatment of dye wastewater. However, improving the water flux of 2D material-based membranes remains a challenge. In this work, 2D ZIF-67/CNF membranes were prepared on a polyether sulfone (PES) support layer via a simple extraction filtration method. The added CNF intertwined with 2D ZIF-67 nanosheets and formed an interlocked stacked laminar flow structure, which improved both the water flux and stability of the thin-film nanocomposite (TFN) membranes. The optimized membrane showed a water flux of 357.8 L m − 2 h − 1 bar − 1 , and its rejections of four dyes (Direct Red 80, Methyl Blue, Alkali Blue 6B and Congo Red) were all above 99.8 %. Furthermore, the optimized membrane maintained a rejection of 97 % after 8 h of continuous operation, indicating that the facile mixing of nanofibers into nanosheets can be considered a promising strategy for preparing TFN membranes for dye wastewater treatment.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Dezhi Zhao,Hexiang Wang,Zheng Wang,Shuang Lu
Amidoxime groups were successfully introduced to develop a novel amidoxime-functionalized cellulose fiber (AO-Cell) for absorptive removal of heavy metal ions in wastewater. The chemical structure , and the competitive adsorption of Cu 2+ and Zn 2+ by AO-Cell were investigated by experiments study, Density functional theory (DFT) and molecular dynamic (MD) simulation. The results showed the N and O atoms in the amidoxime group can spontaneously interact with Cu 2+ and Zn 2+ through sharing long pair electrons to generate stable coordination structure, which was the dominant adsorption mechanism. Besides, the enlarged surface area, improved hydrophilicity and dispersion offered by AO-Cell facilitate the adsorption process by increasing the accessibility of absorption sites. As results of these synergetic modification, AO-Cell can remain effective in a wide pH range (1–6) and reach adsorption equilibrium within 60 min. At optimal conditions, the achieved theoretical adsorption capacity is as high as 84.81 mg/g for Cu 2+ and 61.46 mg/g for Zn 2+ in the solution with multiple ions. The competition between Cu 2+ and Zn 2+ in occupying the absorption sites arises from the difference in the metallic ion affinity and covalent index with the adsorbent as demonstrated by the MD analysis. Importantly, AO-Cell demonstrated favorable recyclability after up to 10 adsorption-desorption cycles.
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ACS Applied Polymer Materials
·
2024
·
IF 4.4
Zhi Li,Kai Shen,Miao Liu,Min Hu,Shengjie Yao,Qiong Liu,Chuangang Fan,Xiaoxu Wu
Oil–water separation has attracted widespread attention from researchers because of increasing worldwide water pollution. Therefore, it is urgent to design an environmentally friendly material with a high efficiency oil–water separation property. Herein, a facile strategy for fabricating ultralight, biodegradable, and high porosity methyltrimethoxysilane (MTMS) modified poly(vinyl alcohol) (PVA)/cellulose aerogels (PCAs) by chemical vapor deposition is reported. The obtained results show that the oil–water separation ability of PCA is enhanced with the increase of the PVA load. Besides, the hydrophobicity of the modified PCA is improved relative to that of pristine PCA. In particular, the water contact angle of MTMS-P0.4CA was 140.3°. Furthermore, PCA modified MTMS exhibits an excellent repeated adsorption capacity for organic liquids. The as-prepared aerogels with outstanding hydrophobicity and oil–water separation ability show great potential as recyclable organic liquid captors.
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CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Feiyu Tang,Bin Wang,Jinpeng Li,Jun Xu,Jinsong Zeng,Wenhua Gao,Kefu Chen
Water-soluble silver nanoclusters (AgNCs) as a new type of fluorescent material have attracted much attention for their remarkable optical properties and excellent cytocompatibility. However, it is still challenging to synthesize water-soluble AgNCs with good cytocompatibility and excellent fluorescence. Herein, the dialdehyde nanofibrillated cellulose (DANFC)- reduced water-soluble AgNCs capped by glutathione (GSH) with tunable fluorescence emissions were first reported. The DANFC provides a mild reduction environment and crystal growth system for the coordination between silver ions and GSH compared to conventional methods using strong reducing agents. The AgNCs with intense red fluorescence (R-AgNCs@GSH, size ∼2.24 nm) and green fluorescence (G-AgNCs@GSH, size ∼1.93 nm) were produced by varying the ratios of silver sources and ligands, and could maintain stable fluorescence intensity over 6 months. Moreover, the CCK-8 study demonstrated that the R-AgNCs@GSH and G-AgNCs@GSH reduced by DANFC of excellent cytocompatibility (cell viability >90 %) and enable precise multicolor intracellular imaging of Hela cells in 1 h. This work proposes a novel method to synthesize water-soluble AgNCs with tunable fluorescence emission at room temperature based on the classical silver- mirror reaction (SMR) using DANFC as reducing agent, and the synthesized fluorescent AgNCs have great potential as novel luminescent nanomaterials in biological research.
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BIOMASS & BIOENERGY
·
2024
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IF 5.8
Yufa Gao,Wenlu Zhang,Mohammad Ali Asadollahi,Chao Liu,Guang Yu,Haiming Li,Bin Li
Clean and efficient pretreatment is a critical process for producing cellulosic sugar platform for the production of advanced biofuels and chemicals. Combining a chemical treatment with ball milling is a relatively simple and environmentally friendly procedure, which may reduce the pretreatment time and energy consumption. Hence, in this study, the mineral salts (e.g. ZnCl 2 , ZnBr 2 , LiCl, and LiBr) were used to enhance the ball milling. Firstly, microcrystalline cellulose (MCC) as a model compound of pure cellulose was pretreated by mineral salt-assisted ball milling to investigate the effect of mineral salt and ball milling for promoting cellulose saccharification. LiBr showed the best effect to improve the effectiveness of ball milling pretreatment, and the glucose yield of saccharification reached up to 93.8 % under the optimal conditions. Then, corncob residue (CCR) as a distinctive starting material was pretreated by LiBr-assisted ball milling, and the corresponding glucose yield of saccharification (48 h) with enzyme dosage of 5 FPU/g-CCR was as high as 97.4 %. Characterization verified that, the effect of LiBr and ball milling could more effectively break hydrogen bonds and crystalline structure of cellulose, thus highly promoting the followed enzymatic saccharification without lignin removal and washing after LiBr-assisted ball milling pretreatment. This work can help to understand the effect of combining mineral salt with ball milling, which is beneficial to the development of clean and effective pretreatment approach for lignocellulose biorefinery.
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