Food and Bioprocess Technology
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2026
·Vol. 19
·期 3
·163
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IF 5.6
Xia Shiqi,Gao Tongxin,Cui Congli,Bi Chong-hao,Li Dong,Wang Li-jun
Decolorization is critical in sugar refining, where reducing sugar alkaline degradation products (RSADPs) constitute major colorants. Conventional decolorants face limitations including non-renewability, high cost, and narrow applicability. This study developed quaternary ammonium-functionalized chitosan adsorbents (QA) as high-performance and cost-effective alternatives. QA was synthesized via (3-chloro-2-hydroxypropyl) trimethylammonium chloride modification, followed by chemical cross-linking with glutaraldehyde and freeze-drying. QA displayed a more intricate surface morphology with rougher pore walls, while increasing quaternary ammonium-functionalized chitosan (QACS) content contributed to the preservation of the porous framework. Additionally, QA exhibited a stable positive charge (pH 3–11) and industrial-grade thermal stability. Compared to the unmodified chitosan adsorbent, QA exhibited superior adsorption performance and maintained pH-independent functionality, with adsorption capacity increasing with rising QACS content. QA 5 (with 5 wt.% quaternary ammonium-functionalized chitosan) performed optimally, achieving 95.9% RSADPs removal with 172.6 mg/g equilibrium capacity (theoretical max of 287.7 mg/g) and > 15% higher efficiency than unmodified adsorbent. The adsorption kinetics followed the pseudo-second-order model (chemisorption-dominated), and the isotherms fitted the Freundlich model, indicating multilayer heterogeneous adsorption. The QA thus provides an efficient, eco-friendly solution with broad pH tolerance for industrial sugar decolorization.
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Journal of Analytical Science and Technology
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2026
·Vol. 17
·期 1
·12
·
IF 2.4
Chen Lingling,Chen Chen
Non-small cell lung cancer (NSCLC) is a leading cause of global cancer mortality, with a poor five-year survival rate primarily attributed to late diagnosis and therapeutic resistance. Emerging evidence highlights the critical role of microRNAs (miRNAs) in NSCLC pathogenesis, progression, and prognosis. However, conventional polymerase chain reaction (PCR)-based miRNA detection methods often lack sufficient sensitivity and robustness. This study presents a novel, self-assembled bio-sensing platform for the highly sensitive analysis of circulating NSCLC-associated miRNAs. The platform integrates a target-primed hybridization chain reaction (THSP) with cooperative self-primed circular amplification, utilizing a Variable Hairpin Probe (VHP) biosensor coupled with the SYBR Green I fluorescent dye system. Upon specific recognition of target miRNA-21, the system initiates a cascade amplification, minimizing nonspecific background. The activated reaction generates abundant double-stranded DNA products, which bind SYBR Green I to produce a strong fluorescent signal without requiring expensive labeled probes. This straightforward method achieves a detection limit of 1.33 fM, significantly enhancing the sensitivity and practicality. It offers a promising strategy for developing highly specific nucleic acid analysis tools, advancing non-invasive miRNA detection toward precise and personalized NSCLC management.
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Scientific Reports
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2026
·
IF 4.9
Zhu Anming,Liu Junxi,Liu Youyun,Xu Rui,Yuan Yonglei,Sun Qiwen,Wang Feifei,Qu Liping
Metapanax delavayi (Franch.) J. Wen & Frodin (Araliaceae) is a plant with both medicinal and edible value, but there is no study examining its effects on skin neurogenic inflammation. We investigated the anti-neurogenic inflammatory and soothing potential of Metapanax delavayi extract (MDE) in vitro using SH-SY5Y cells activated by capsaicin and HaCaT cells stimulated by substance P, and in vivo using a heat-induced zebrafish model. We found that MDE significantly inhibited TRPV1 activation and intracellular calcium influx, down-regulated neuropeptides (CGRP, NGF, substance P) and proinflammatory cytokines (IL-1α, IL-6, IL-8), and regulated emotion-related hormones (decreased cortisol, increased β-endorphin). It also reduced heat-induced hypermotility in zebrafish. Critically, the mechanistic study suggests that biological activity may be associated with the inactivation of the CRHR1/POMC/MC1R signaling pathway. Collectively, these findings indicate that MDE possesses significant potential as a natural skincare ingredient for counteracting neurogenic inflammation associated with sensitive skin.
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Science China-Life Sciences
·
2026
·
IF 9.6
Wang Bao,Fu Junpeng,Qian Jian,Wei Yuang,Bao Meiling,Miao Haoqi,Zhao Xinzhe,Shao Kezheng,Song Yuan,Tan Ruoyun,Li Jie,Shao Pengfei
Clear cell renal cell carcinoma (ccRCC) exhibits aberrant lipid synthesis, yet how this metabolic reprogramming influences ferroptosis—a form of regulated cell death driven by lipid peroxidation—remains poorly understood. This study demonstrates that the key lipid transcription factor SREBP1 acts as a master regulator that promotes ccRCC progression by suppressing ferroptosis. Using SREBP1-knockdown cell models, we found that SREBP1 depletion enriches ferroptosis-related pathways and enhances ferroptosis sensitivity, as evidenced by elevated mitochondrial ROS, labile iron pool, PE-OOH levels, and characteristic mitochondrial alterations. Mechanistically, SREBP1 transcriptionally represses the pro-ferroptotic gene ACSL4 while simultaneously activating the lipid-synthesis gene FASN . Rescue experiments revealed that modulating either ACSL4 or FASN alone only partially reverses the ferroptosis phenotype induced by SREBP1 loss, whereas dual manipulation of both genes fully restores ferroptosis resistance. Metabolomic analyses showed that ACSL4 and FASN converge to regulate the cellular PUFAs/MUFAs ratio, which in turn feeds back to modulate SREBP1 nuclear translocation, thereby establishing a self-reinforcing positive-feedback loop. Clinically, low ACSL4 expression correlates with advanced tumor stage and poor patient survival. In vivo, SREBP1 promotes tumor growth by inhibiting ferroptosis via the ACSL4 / FASN axis. Collectively, our findings reveal that SREBP1 drives ccRCC malignancy through a dual-pronged mechanism—repressing ACSL4 and activating FASN—to alter the PUFAs/MUFAs balance and establish a feed-forward loop that sustains ferroptosis suppression. ACSL4 represents a potential prognostic biomarker, and targeting the SREBP1-ACSL4 / FASN axis offers a promising therapeutic strategy for ccRCC.
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JOURNAL OF NANOBIOTECHNOLOGY
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2026
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IF 15
Na Yang,Chunli Wang,Jiaxi Wang,Jikai Xia,Qingyong Cao,Henan Cao,Yingying Wang,Chunmin Bo,Shukai Ge,Chengjie Sun,Zhongyuan Cai,Hongyu Lin,Kun Liu
Real-time and accurate detection of drug-induced liver injury is critical for early intervention and treatment, yet clinically applicable visualization methods remain scarce. Based on the difference in glutathione (GSH) content between the normal liver and the livers with different-grade injury, we report a GSH-activated T 1 -weighted magnetic resonance imaging (MRI) nanoprobe (C-MnO), which can be used for non-invasive real-time in vivo magnetic resonance imaging (MRI) of the consumption of GSH in the liver, thereby being applied for the graded diagnosis of liver injury. Due to the presence of abundant disulfide bonds in C-MnO, the T 1 MRI signal around it remained "quenched" until encountering GSH. When C-MnO enters the body and is efficiently absorbed by the liver, it will disassemble and degrade under the action of GSH, thereby activating the T 1 MRI signal. Therefore, this nanoprobe provides an effective visual method for capturing the changes in GSH content during different degrees of liver damages at an early stage, which is beneficial for the graded diagnosis and precision treatment of drug-induced liver injury. Comprehensive in vitro and in vivo studies demonstrate that C-MnO, as a GSH-activated T 1 MRI nanoprobe, enables real-time monitoring and graded diagnosis of drug-induced liver injury, effectively addressing current clinical limitations in the detection of liver injury.
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JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
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2026
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IF 3.4
Li Xiao,Sun Hanju,Nagassa Merga,Gao Lingyan,Pei Hui,Liu Shuyun,Gu Yingying,He Xinzhou,He Shudong
In this study, a kind of instant kudzu root powder was prepared by twin-screw extrusion combined with ultrafine grinding. Extruded kudzu root powder (EK) and ultrafine extruded kudzu root powder (UEK) were prepared by twin-screw extruder and vibrating ultrafine pulverizer respectively. The effects of extrusion and ultrafine grinding on physicochemical properties, starch composition and instant solubility of kudzu root were revealed. Compared with that of raw powder, the gelatinization degree of EK increased, and that of RDS increased by 5.41%. The content of RS and SDS decreased by 1.25% and 9.18%, respectively. The particle size of UEK decreased significantly after ultrafine grinding, with an average particle size of 11.29–20.99 μm. The particle size was minimum (11.29 μm) after ultrafine grinding for 10 min. The microstructure diagram indicated that after extrusion and ultrafine grinding, the particles of EK and UEK presented in many amorphous flake structures. Infrared spectrum implied that kudzu root was transformed into amorphous structure. Compared with the control, the ΔH and T p of EK and UEK decreased. Compared with control, the instant solubility of the powder was improved after extrusion and ultrafine grinding.
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METALLURGICAL AND MATERIALS TRANSACTIONS B-PROCESS METALLURGY AND MATERIALS PROCESSING SCIENCE
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2026
·
IF 3.3
Chen Buxin,Li Yehui,Chen Mao,Liu Lingling,Ma Kaihui,Bai Chenguang,Hu Meilong
To support green transition of vanadium titanomagnetite blast furnace smelting, various low-carbon blast furnace atmospheres, including oxygen-enriched air injection (CO–N 2 ), oxygen-enriched H 2 -rich air injection (CO–H 2 ), and H 2 -rich air injection (CO–H 2 –N 2 ) were investigated to gain insight on the Ti(C,N) formation mechanisms. In this study, thermogravimetric analysis was first employed to investigate the kinetics of the TiO 2 reaction. Arrhenius-based kinetic modeling revealed that these reactions are primarily controlled by solid-state diffusion, and N 2 directly nitrides titanium oxides to TiN, and the activation energy decreases with increasing N 2 content. In contrast, H 2 lowers the activation energy of the reaction and promotes the reduction of TiO 2 to lower-valence oxides. Then, the carbothermic reduction of TiO 2 -bearing slags was investigated. Chemical composition analysis, XRD, and EPMA-WDS mapping demonstrated pronounced atmosphere-dependent variations in the formation of TiC/N phases. In CO–N 2 atmospheres, Ti(C,N) content decreases with increasing CO content and Ti(C,N) tends to agglomerate. With pure CO, only a few, sparsely dispersed TiC particles were detected. Whereas in CO–H 2 atmospheres, increasing the H 2 concentration markedly enhanced TiC formation, yielding uniformly dispersed particles with slight clustering. Therefore, the overall atmosphere effect on the amount of TiC/N formation follows the order: 100 pct CO < 80 pct CO–20 pct H 2 < 80 pct CO–20 pct N 2 < 60 pct CO–40 pct H 2 < 60 pct CO–40 pct N 2 < 30 pct CO–70 pct N 2 atmosphere (conventional blast furnace atmosphere). Graphical
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CATALYSIS LETTERS
·
2026
·Vol. 156
·期 3
·76
·
IF 2.5
Khan Khyrullah,Wang Wei,Chang Zhidong,Dong Bin
The innovation for highly effective solid acid catalysts that resist impurities is crucial for enhancing sustainable esterification and transesterification processes, which are vital for biofuel production and green chemistry. In this work, tungsten-modified sulfated titania (WO₃/SO₄ 2 ⁻/TiO₂) catalyst was prepared through the impregnation method and subsequently calcined at 550 °C to assess the impact of tungsten impurity on its structural and catalytic properties. The catalyst was characterized through FT-IR, XRD, NH 3 -TPD, and BET surface area analysis. These analyses revealed that the presence of sulfate and tungsten species inhibited TiO₂ crystallization, improving the textural properties and increasing surface acidity. The 5 wt.% WO₃/SO₄ 2 ⁻/TiO₂ catalyst demonstrated the largest surface area (35.99 m 2 /g) and total acidity (1.20 mmol NH₃/g). This catalyst achieved 86.4% conversion in the reaction of transesterification of ethyl acetate and n-butanol at 100 °C for 3 h. The enhanced catalytic performance and selectivity were attributed to the synergistic interaction between WO₃ and SO₄ 2 ⁻, resulting in the formation of Lewis-Bronsted acid sites. This study provides important insights into how tungsten affects the structure and acidity of sulfated titania, which could guide the design of more efficient, impurity-tolerant solid acid catalysts for environmentally sustainable chemical processes. Graphical
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Nature Communications
·
2026
·Vol. 17
·期 1
·2945
·
IF 18.1
Qiu Ruiying,Huang Huisi,Chi Junxi,Gao Longwei,Gao Qilin,Li Min,Cai Menghao,Yu Haishuang,Wang Shijie,Qian Zhilan,Jiang Minchuan,Liu Yu,Chen Bo,Zhou Yang,Zhang Zhi-Min,Wang Jian-bo
C -prenylated flavonoids possess notable pharmaceutical potential, but their production is hindered by the challenging selective prenylation of their unstable polyphenolic cores. Natural prenyltransferases offer a direct route but suffer from low activity and incomplete mechanistic understanding. Here, we report a directed evolution strategy to reshape the active pocket of the prenyltransferase AtaPT, uncovering an aromatic cage that governs both regioselectivity and donor specificity. By tuning cage occupancy, we engineer three mutants with high chemo- and regioselectivity toward dimethylallyl diphosphate or geranyl pyrophosphate. Structural analysis and molecular simulations validate the role of the cage in guiding flavonoid prenylation. Notably, the aromatic cage mechanism observed in AtaPT is not unique and can be recapitulated in homologous enzymes. Introduction of the aromatic cage consistently enhances both activity and selectivity, confirming its crucial role. AtaPT mutants enable the efficient and scalable synthesis of 27 C -prenylated flavonoids, including 8 previously unreported compounds. With an integrated donor regeneration system, preparative-scale biotransformations achieve product titers up to 400 mg/L. This study establishes a selective and scalable biocatalytic platform for flavonoid prenylation and offers mechanistic insights for enzyme engineering.
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BMC Chemistry
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2026
·
IF 5.1
Shi Xingyi,Cai Huahuan,Tang Wei,Wei Erjun,Zhao MingXiao,Zhao Yufen
The evolutionary origin of copper-binding proteins remains a central question in prebiotic chemistry. Modern copper proteins predominantly coordinate copper through histidine and cysteine residues, suggesting that these amino acids were selectively favored during early protein evolution. Here, we propose that ultraviolet (UV) radiation acted as a key selective factor in shaping primordial copper-peptides. Spectroscopic analyses suggest that His 2 interacts with Cu(II) through contributions from both its N- and C-terminal donor groups, generating a comparatively stronger binding environment and supporting its plausibility as a primitive Cu-binding motif. Yet His 2 is intrinsically vulnerable to UV-induced degradation, highlighting the need for stabilizing partners. In contrast, Tyr 2 exhibits remarkable photostability and preferentially binds Cu(II) through its N-terminal, consistent with a second-shell protective role analogous to those observed in modern Cu-binding proteins. Together, these findings suggest that Earth’s UV-rich environment favored cooperative copper-binding dipeptides, where His₂ provided catalytic functionality while Tyr 2 contributed photostability, establishing a plausible pathway from simple Cu-peptide complexes to functional metalloenzymes.
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Science China-Materials
·
2026
·
IF 7.7
Zeng Jie,Xiong Feilong,Zan Jun,Wang Dongan,Peng Shuping,Yang Hejin,Shuai Cijun
Bacterial infection following endometrial injury would delay tissue regeneration and further progress to endometritis and other reproductive disorders. Photodynamic therapy (PDT) is a promising bacterial strategy in the postantibiotic era. However, its therapeutic efficacy is often limited by the rapid recombination of photogenerated charge carriers and the limited penetration depth of visible excitation light. In this study, we report a previously unexplored up-conversion-mediated, Type I dominant photodynamic antibacterial uterine scaffold specifically designed for infection-associated endometrial injury. The core innovation lies in the construction of a Schottky Ag 0 -Ag 2 S heterojunction on the surface of NaYF 4 :Yb,Tm nanoparticles using ZIF-8 as a sacrificial precursor, which enabled highly efficient charge separation and oxygen-independent hydroxyl radical generation, effectively overcoming the inherent oxygen dependence and compromised performance of conventional Type II PDT under hypoxic uterine conditions. The upcon-version core further enables deep-tissue-penetrable NIR activation, addressing the poor penetration of visible light. Moreover, beyond instantaneous PDT, the dynamic release of Ag + ions provides synergistic bactericidal activity, enabling spatiotemporally coordinated biofilm disruption. Importantly, these nanostructures were incorporated into patient-customizable, biodegradable PLLA scaffolds fabricated by selective laser sintering, achieving simultaneous antibacterial therapy and endometrial regeneration in a single platform. Collectively, the integration of an oxygen-independent PDT mechanism, MOF-templated heterojunction engineering, and 3D printed personalized uterine implants constitutes a comprehensive therapeutic strategy not previously reported for treating infection and endometrial injury. The alternative text for this image may have been generated using AI.
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CARDIOVASCULAR DRUGS AND THERAPY
·
2026
·
IF 3.5
Chen XinJing,Hong Jingxuan
Objective This study aimed to determine whether dexmedetomidine (DEX) attenuates myocardial ischemia/reperfusion injury (MIRI) by regulating DNA methyltransferase 3B (DNMT3B)–mediated methylation of a CpG island within the Kruppel-like factor 4 (KLF4) promoter. Methods A rat model of MIRI was established and treated with DEX, DNMT3B knockdown, or DEX combined with DNMT3B overexpression. Histopathological alterations, apoptosis, and DNMT3B/KLF4 expression were assessed using H&E staining, TUNEL/cTnT co-staining, RT-qPCR, western blot, immunofluorescence, and immunohistochemistry. H9C2 cardiomyocytes subjected to oxygen-glucose deprivation/reperfusion (OGD/R) were treated with DEX and transfected with DNMT3B or KLF4 knockdown/overexpression plasmids. CpG island methylation within the KLF4 promoter was predicted using MSP and ChIP-PCR. Results MIRI impaired cardiac function, increased infarct size and myocardial enzyme release, upregulated DNMT3B expression, downregulated KLF4 expression, and promoted cardiomyocyte apoptosis. DNMT3B was predominantly localized in cTnT-positive cardiomyocytes. DEX treatment or DNMT3B knockdown reduced DNMT3B expression, restored KLF4 levels, and alleviated myocardial injury in vivo as well as OGD/R-induced damage in vitro . DEX was associated with decreased methylation of the investigated CpG island within the KLF4 promoter. DEX may inhibit DNMT3B-mediated promoter hypermethylation, thereby enhancing KLF4 transcription. KLF4 knockdown abolished the cardioprotective effects of DEX, while DNMT3B overexpression reversed DEX-mediated improvements in vivo . Conclusions DEX may attenuate MI/RI by downregulating DNMT3B and reducing methylation at a CpG island within the KLF4 promoter, thereby promoting KLF4 expression. However, the intrinsic resolution limitations of MSP and ChIP-PCR restrict precise mapping of methylation sites. Higher-resolution techniques, such as whole-genome bisulfite sequencing or single-molecule methylation sequencing, are warranted to validate these findings.
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