CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2020
·Vol. 36
·期 2
·301-306
·
IF 1.063
Liu Shuo,Yang Mingjie,Guo Weiwei
Metal-organic frameworks(MOFs)-based nanozyme plays an important role in biosensing, therapy and catalysis. In this study, the effects of single-stranded DNA(ssDNA) with programmable sequences and its complementary DNA(TDNA) on the intrinsic peroxidase-like activity of hemin loaded MOFs(UiO-66- 2 ), denoted as hemin@UiO-66-NH 2 , were investigated. The hemin@UiO-66- 2 exhibited improved catalytic activity compared with free hemin. However, the catalytic activity is inhibited in the presence of ssDNA, as ssDNA can be adsorbed by MOFs and therefore protected the active sites from contact with substrates. Upon the addition of the T DNA , double-stranded DNA(dsDNA) was formed and detached from the MOFs, resulting in the recovery of catalytic activity. Sequentially adding ssDNA or its complementary DNA strands can achieve the reversible regulation of the catalytic activity of MOFs nanozymes. Moreover, the DNA hybridization-based regulation was further applied to a cascaded catalytic system composed of the nanozyme, hemin@UiO-66-NH 2 , and glucose oxidase. These nanozyme based programmable and reversibly regulated catalytic systems may have potential applications in future smart biosensing and catalysis systems.
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Scientific Reports
·
2017
·Vol. 7
·期 1
·6783-6783
·
IF 4.259
Fang Liu,Na Zhang,Zhaojie Li,Xiong Wang,Hongjie Shi,Changhu Xue,Robert W. Li,Qingjuan Tang
Chondroitin sulfate (CS) has been widely used for medical and nutraceutical purposes due to its roles in maintaining tissue structural integrity. We investigated if CS disaccharides may act as a bioactive compound and modulate gut microbial composition in mice. Our data show that CS disaccharides supplementation for 16 days significantly reduced blood LPS in the mice experiencing exhaustive exercise stress. CS disaccharides partially restored total fecal short-chain fatty acids from the level significantly repressed in mice under the stress. Our findings demonstrated that CS was likely butyrogenic and resulted in a significant increase in fecal butyrate concentration. CS disaccharides had a profound impact on gut microbial composition, affecting the abundance of 13.6% and 7.3% Operational Taxonomic Units in fecal microbial communities in healthy and stressed mice, respectively. CS disaccharides reduced the prevalence of inflammatory Proteobacteria. Together, our findings demonstrated that CS may ameliorate stress-induced intestinal inflammation. Furthermore, CS significantly increased intestinal Bacteroides acidifaciens population, indirectly exerting its immunomodulatory effect on the intestine. CS disaccharides had a significant impact on a broad range of biological pathways under stressed condition, such as ABC transporters, two-component systems, and carbohydrate metabolism. Our results will facilitate the development of CS as a bioactive nutraceutical.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2020
·Vol. 27
·期 22
·27849-27858
·
IF 3.056
Ou Qi,Xu Shuxia,Long Yuanli,Zhang Xinfeng
The development of efficient solar driven catalyst for the degradation of antibiotics has become increasingly important in environmental protection. However, the reported efficient photocatalysts for antibiotic degradation are limited. In this work, porous Fe 3+ -doped graphitic carbon nitride (g-C 3 N 4 ) with outstanding photocatalytic ability is synthesized and then used as the photocatalyst for the efficient degradation of sulfadiazine (SDZ) under visible light. A series of characterization results indicate that Fe 3+ is successfully doped into the interlayer of g-C 3 N 4 and is stabilized in g-C 3 N 4 by Fe-N coordination bond. The SEM, DRS and ESI and transient photocurrent results demonstrated that Fe 3+ -doped g-C 3 N 4 has a porous structure, a low band gap, improved separation efficiency of photogenerated electron and holes as well as a wider light absorption range. Such improved physical and chemical properties greatly enhanced the photocatalytic ability. Using Fe 3+ -doped g-C 3 N 4 for photocatalytic degradation of SDZ under white light, almost complete degradation of SDZ was achieved with a degradation efficiency as high as 99.8% (whereas only 52.1% for bulk g-C 3 N 4 ) within 90 min. The degradation was mainly ascribe to 1 O 2 during the irradiation, and also a small amount of •O 2 − , OHand h + are involved in the degradation process. The Fe 3+ -doped g-C 3 N 4 was applicable for the degradation of a wide range of antibiotic pollutants.
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BIOTECHNOLOGY LETTERS
·
2016
·Vol. 38
·期 6
·1009-1013
·
IF 1.639
Kang Le,Bai Yajun,Cai Yujie,Zheng Xiaohui
Objectives To characterize a novel feruloyl esterase from Escherichia coli BL21 DE3. Results The gene encoding BioH was cloned and overexpressed in E. coli . The protein was purified and its catalytic activity was assessed. BioH exhibited feruloyl esterase activity toward a broad range of substrates, and the corresponding kinetic constants for the methyl ferulate, ethyl ferulate, and methyl p -coumarate substrates were: K
m
values of 0.48, 6.3, and 1.9 mM, respectively, and k
cat
/ K
m
values of 9.3, 3.8, and 3.8 mM −1 s −1 , respectively. Conclusions Feruloyl esterase from E. coli was expressed for the first time. BioH was confirmed to be a feruloyl esterase.
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AAPS PHARMSCITECH
·
2018
·Vol. 19
·期 5
·2048-2057
·
IF 2.666
Zhai Qingqing,Li Hailong,Song Yanlin,Wu Ruijiao,Tang Chuanfang,Ma Xiaodong,Liu Zhihao,Peng Jinyong,Zhang Jianbin,Tang Zeyao
This work aimed to develop and optimize several lipid nanocapsule formulations (LNCs) to encapsulate cisplatin (CDDP) for treatment of hepatocellular carcinoma. By comparing the effect of oil/surfactant ratio, lecithin content, and oil/surfactant type on LNC characteristics, two LNCs were selected as optimal formulations: HS15-LNC (Solutol HS 15/MCT/lecithin, 54.5:42.5:3%, w / w ) and EL-LNC (Cremophor EL/MCT/lecithin, 54.5:42.5:3%, w / w ). Both LNCs could effectively encapsulate CDDP with the encapsulation efficiency of 73.48 and 78.84%. In vitro release study showed that both LNCs could sustain the release CDDP. Moreover, cellular uptake study showed that C6-labeled LNCs could be effectively internalized by HepG2 cells. Cellular cytotoxicity study revealed that both LNCs showed negligible cellular toxicity when their concentrations were below 313 μg/mL. Importantly, CDDP-loaded LNCs exhibited much stronger cell killing potency than free CDDP, with the IC50 values decreased from 17.93 to 3.53 and 5.16 μM after 72-h incubation. In addition, flow cytometric analysis showed that the percentage of apoptotic cells was significantly increased after treatment with LNCs. Therefore, the prepared LNC formulations exhibited promising anti-hepatocarcinoma effect, which could be beneficial to hepatocellular carcinoma therapy.
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JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2021
·Vol. 25
·期 3
·1073-1081
·
IF 2.647
Jin Xin,Lv Mengmeng,Pan Qing,Fang Shu,Zhu Ningning
We describe a label-free electrochemical aptasensor for adenosine triphosphate (ATP) assay based on bifunctional Fe 3 O 4 @Au nanocomposites. Due to the introduction of Au nanoparticles, the resulting bifunctional Fe 3 O 4 @Au nanocomposites can be easily modified with ATP-specific aptamer molecules through Au–S interaction to build the nanoprobe and used to immobilize on a glassy carbon electrode simply with the assistance of a magnet. Once exposed to the target ATP, the current signal obtained at nanoprobe-containing magnetic glassy carbon electrode decreases immediately. The decrease in sensor current is a result of the less methylene blue adsorbed on the aptamer caused by the fact that the ATP-specific aptamer prefers to bind with ATP rather than with methylene blue. The decrease in sensor current can be used for electrochemical determination of ATP, for example, to determine ATP in human serum samples.
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MICROCHIMICA ACTA
·
2016
·Vol. 183
·期 12
·3201-3208
·
IF 4.831
Lan Jianming,Li Li,Liu Yingxin,Yan Liu,Li Chunyan,Chen Jinghua,Chen Xiaosong
Upconversion nanoparticles (UCNPs) of the type α-NaYF 4 :Yb 3+ , Er 3+ and a typical diameter of 6–7 nm were synthesized by thermal decomposition of the respective rare-earth stearate. The oleic acid on the surface of the UCNPs was then replaced by aptamer DNA. The assay was performed in a microplate format with a capture probe immobilized in the wells. Following binding of the vascular endothelial growth factor (VEGF), an auxiliary probe DNA is added that is labeled with UCNPs and binds to the VEGF-loaded capture probe. The method enables highly sensitive and highly specific detection of the VEGF which is a marker for breast cancer. Under the optimum conditions, the intensity of the upconversion luminescence (at excitation/emission wavelengths of 980/541 nm) is linearly proportional to the VEGF concentration in the 50 pM to 2000 pM concentration range, with a 6 pM detection limit. The method was applied to the determination of VEGF in spiked serum, typically at a 500 pM level, and gave recoveries that ranged from 98 to 113 %, with RSDs between 2.9 and 3.6 %. This makes it a viable tool for early diagnosis of breast cancer.
An upconversion luminescence based assay is presented for trace analysis of vascular endothelial growth factor. It is based on a sequence fragment-linked technique of target-induced aptamer (AP) and upconversion nanoparticles (UCNPs) and has potential application in the early diagnosis of breast cancer.
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JOURNAL OF MATERIALS SCIENCE
·
2018
·Vol. 53
·期 13
·9368-9381
·
IF 2.993
Liu Le,Li Tuanwei,Ruan Zheng,Yan Lifeng
Photodynamic therapy (PDT) combining with near infrared (NIR) imaging is attractive. However, the intrinsic hypoxia in tumor and consumption of oxygen during treatment will decrease PDT. Here an artificial red cell was prepared using polypeptides conjugated hemoglobin as an oxygen carrier. A NIR photosensitizer-brominated 4,4-difluoro-4-bora-3a,a-diaza-s-indacene (BODIPY-Br 2 ) possessing both high fluorescence emission and singlet oxygen generation efficiency was synthesized and also conjugated to polypeptides to achieve NIR imaging-guided PDT. In vitro studies performed on HepG2 cancer cells verified the oxygen carrier, cancer tracing and curing abilities of the as-prepared polymeric nanoparticles. Even under hypoxia condition, it also obviously increases the cell killing rate when exposed light at a low energy (25 mW/cm 2 , 10 min). Meanwhile, the fluorescence of BODIPY in NPs would light up cells for real-time imaging. These results show the potential of the biocompatible and biodegradable P-Hb-B NPs for enhancement of simultaneous tracing and treating of cancer.
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Journal of Superconductivity and Novel Magnetism
·
2025
·Vol. 38
·期 2
·112
·
IF 1.7
Li Xiaofeng,Gao Yuxia,Tian Zhaoming,Ren Weijie,Sun Xin,Wang Mingli
KNi(PO 3 ) 3 was successfully synthesized by solid-state method and the magnetic properties were characterized. The crystal structure of KNi(PO 3 ) 3 belongs to trigonal crystal system with space group R 3, where Ni 2+ ions form the distorted honeycomb-lattice within the ab-plane stacking in the “ABAB” type fashion along the c -axis. Magnetization measurements reveal the presence of antiferromagnetic (AFM) interaction with Curie Weiss (CW) temperature θ CW = − 12.905 K but without long-range magnetic order down to 2 K. Notably, the CW fitted effective moment µ eff = 3.35µ B and saturated magnetization M S = 2 μ B /Ni 2+ at 2 K support KNi(PO 3 ) 3 as an S = 1 spin system. Intriguingly, the zero-field specific heat exhibits a broad peak maximized at ~ 2.5 K indicating the onset of short-range spin correlation between Ni 2+ ions, while the integrated magnetic entropy ( S mag ) is close to the expected Rln3 for S = 1 system, indicative of large spin fluctuation below 2 K driven by spin frustration.
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RARE METALS
·
2025
·
IF 11
Dong Fei-Long,Liu Zhen-Hua,Jiang Ying,Liang Zhe,Chu Xu,Ma Chun-Hui,Sun Hao,Xie Hai-Ming
TiNb 2 O 7 (TNO) is regarded as a potential fast-charging anode for lithium-ion batteries due to its fast reversible phase transition and safe working potential, but it suffers from unsatisfactory electronic conductivity, sluggish ion kinetics, and poor structural stability under high rates. Herein, tantalum (Ta) with strong Ta–O bonds is doped into TNO to enhance its fast-charging property and structural stability. Proper amount of Ta doping not only increases the interlayer spacing for fast Li + transport, but also improves the electrical conductivity, being successfully proved by experimental analyses. Density functional theory (DFT) calculation verifies that the lattice distortion induced by the incorporation of strong Ta-O bonds can effectively strengthen the structural stability. Notably, in situ X-ray diffraction (XRD) technology reveals that Ta doping relieves volumetric strain during lithiation and de-lithiation. Thus, the optimized Ta 0.1 -TNO sample owns impressive long-term cycling stability even at a high current density of 10C, which delivers a high-capacity retention of 85.12% (a capacity decay of less than 0.01% per cycle) after 1500 cycles. This work provides a fast-charging anode material with superior structural stability for lithium-ion batteries. Graphical abstract
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IRANIAN POLYMER JOURNAL
·
2025
·
IF 2.8
Lai Yiming,Wang Xinyu,Xiao Longqiang,Lan Yunhong,Zhao Rui,Hou Linxi
The chemical fixation of CO 2 into high-value cyclic carbonates is of great significance for achieving CO 2 utilization (CCU) technology. In this study, a series of imidazolium-based ionic linkers, designated as IL-2C, IL-3C, IL-4C, IL-5C, and IL-6C, were synthesized using 1-vinylimidazole and five different dibromoalkane linkers. The linker design incorporates flexible alkyl chains, where increasing chain length modulates the distance between imidazolium centers, influencing the resulting polymer network structure. Subsequently, their corresponding poly(ionic liquid) (PILs) porous polymers (PIL-2C, PIL-3C, PIL-4C, PIL-5C and PIL-6C) were synthesized through free radical copolymerization with octavinyl POSS using 2,2'-azobis(2-methylpropionitrile) (AIBN) as the initiator, resulting in crosslinked porous networks. The structure, morphology, and thermal stability of the PILs were characterized by NMR, FTIR, XRD, BET, SEM and TGA. The results indicate that the synthesized poly(ionic liquid) catalysts possess a mixed microporous/mesoporous structure, enhancing their performance in various applications. The PILs were evaluated as catalysts for the cycloaddition of CO 2 to epoxides, with PIL-5C exhibiting the best performance under ambient pressure, solvent-free, and co-catalyst-free conditions, achieving a cyclic carbonate yield as high as 99.9%. Reaction kinetic studies revealed that the POSS-based polymer significantly reduces the activation energy of the CO 2 cycloaddition reaction, and density functional theory (DFT) simulations provide a complete reaction pathway. Furthermore, PIL-5C demonstrated excellent catalytic activity for epoxides of varying sizes. As a heterogeneous catalyst, the POSS-based polymer showed no structural changes after recycling, as confirmed by FTIR and SEM analyses. The catalyst could be reused for 6 cycles while maintaining high catalytic performance. Graphical abstract
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Nature Communications
·
2025
·Vol. 16
·期 1
·11179
·
IF 15.7
Chen Tingxu,Liu Diru,Zhang Mengyuan,He Yueqing,Zhao Lin,Wang Yiying,Wang Qiang,Xu Guangyan,He Hong
Overcoming the trade-off between activity and selectivity has long been a challenge in catalytic reactions. Dual-atom catalysts (DACs) exhibit exceptional catalytic performance in cascade catalysis, owing to the synergistic effects of distinct active sites, which make them particularly promising for enhancing catalytic selectivity. Here, we present dual-atom Ir-W x /CeO 2 catalysts that integrate both oxidation (Ir) and reduction (W) sites for the selective catalytic oxidation of ammonia, a major precursor of air pollutants. Comprehensive characterizations revealed that Ir atoms were embedded on the CeO 2 planes in single-atom form, while W sites were anchored on the CeO 2 surface, forming Ir-W dimer structures. Operando studies and theoretical calculations demonstrated that NH 3 was oxidized on Ir sites, producing NO, which then reacted with NH 3 on W sites via selective catalytic reduction (SCR) to generate N 2 and H 2 O. The synergistic effect of the Ir-W dual-atom dimer significantly enhanced low-temperature activity (≥ 92% at 200 °C) and high-temperature selectivity (≥ 92% at 300 °C) on the Ir-W 7 /CeO 2 catalyst. Furthermore, this dual-atom strategy extends to Ir-Mo/CeO 2 and Ir-Nb/CeO 2 catalysts, demonstrating broad applicability. These findings highlight the potential of DACs for the rational design and application in various cascade catalytic reactions.
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