COLLOIDS AND SURFACES B-BIOINTERFACES
·
2022
·
IF 5.999
Dongdong Gao,Fang Cheng,Xinglong Wang,Heqing Yang,Chong Liu,Chunmei Li,Eva Murong Yang,Gang Cheng,Wei He
Antibody-functionalized nanoparticles (Ab-NPs) are widely used in bioassays due to their excellent affinity, specificity toward antigen, and ease of operation. However, the uncontrollable molecular status of antibodies on NPs severely limits their applications. This work aims at developing a simple method to evaluate the antigen-binding activity of Ab-NPs using two parameters, i.e . , antibody adsorption amount and antigen-binding strength . Herein, we proposed a mathematical expression, G, to quantitively describe the amount and strength of Ab-NPs. G value could be used to assess the antigen-binding performance of NPs influenced by surface and solution factors. Seven types of polymers with different surface properties, including four positively and three negatively charged polymer brushes , were grown from silica NPs via surface-initiated atom transfer radical polymerization (SI-ATRP). A pair of antigen and antibody, human chorionic gonadotropin (hCG) and anti-hCG, were selected to screen the antibody immobilization property of polymer brushes. Among them, the G values of 2 polymer-NPs with opposite charges reached maximum, resulting in low detection limits for hCG, where pDMAEA-NP and pMMA-NP represent Poly[ N,N -(dimethylamino)ethyl acrylate]-NP and poly(methyl methacrylate)-NP, respectively. The G value of Ab-NPs makes it feasible to estimate the molecular status of the adsorbed antibodies on surfaces, thus showing great potential for in vitro biosensing and bioseparation.
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Nanoscale
·
2019
·Vol. 11
·期 39
·18081-18089
·
IF 6.97
Ran Xiong,Wenting Zhang,Yifan Zhang,Ye Zhang,Yimin Chen,Yuan He,Haiming Fan
Remote modulation of nanoscale biochemical processes in a living system using magnetic stimulation is appealing but is restricted by the lack of a highly efficient nanomediator which can deliver timely and effective response to biological molecules under an external magnetic field. Herein, we report the development of a novel nanocatalyst based on a ferrimagnetic vortex-domain nanoring (FVIO)–enzyme hybrid that enables real-time modulation of enzymatic catalysis under an alternating magnetic field (AMF). The role of the FVIO is to provide localized heating immediately upon exposure to an AMF, which efficiently and selectively promotes the activity of conjugated enzymes on the surface. The reaction rate of the as-fabricated FVIO–β-Gal hybrid was shown to be boosted up to 180% of its initial value by localized heat generated under an AMF of 550 Oe in less than 2 s and without heating up the bulk solution. Moreover, the degree of activity acceleration was shown to be tunable by increasing the strength of the AMF. The concept of remote magnetic stimulation of enzymatic reactions has been further applied to other enzymes (e.g. FVIO–KPC and FVIO–GOx), demonstrating the general applicability of this strategy. Since almost all metabolic processes in cells rely on enzymatic catalysis to sustain life, the FVIO–enzyme system developed in this work provides a valuable nanoplatform for spatiotemporally manipulating biochemical reactions, which might pave the way for future remote manipulation of living organisms.
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高影响因子
Nature Protocols
·
2019
·Vol. 14
·期 9
·2672-2690
·
IF 11.334
Ma Wei,Ma Hui,Peng Yue-Yi,Tian He,Long Yi-Tao
Understanding the photoinduced electron-transfer process is of paramount importance for realizing efficient solar energy conversion. It is rather difficult to clarify the link between the specific properties and the photoelectrochemical performance of an individual component in an ensemble system because data are usually presented as averages because of interplay of the heterogeneity of the bulk system. Here, we report a step-by-step protocol to fabricate an ultrasensitive photoelectrochemical platform for real-time detection of the intrinsic photoelectrochemical behaviors of a single entity with picoampere and sub-millisecond sensitivity. Using a micron-thickness nanoparticulate TiO 2 -filmed Au ultramicroelectrode (UME) as the electron-transport electrode, photocurrent transients can be observed for each individual dye-tagged oxide semiconductor nanoparticle collision associated with a single-entity photoelectrochemical reaction. This protocol allows researchers to obtain high-resolution photocurrent signals to quantify the photoinduced electron-transfer properties of an individual entity, as well as to precisely process the data obtained. We also include procedures for dynamic light scattering (DLS) analysis, transmission electron microscopy (TEM) imaging and collision frequency–concentration correlation to confirm that the photoelectrochemical collision events occur at an unambiguously single-entity level. The time required for the entire protocol is ~36 h, with a single-entity photoelectrochemical measurement taking <1 h to complete for each independent experiment. This protocol requires basic nanoelectrochemistry and nanotechnology skills, as well as an intermediate-level understanding of photoelectrochemistry.
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Nature Communications
·
2021
·Vol. 12
·期 1
·1-16
·
IF 14.919
Wang Yongchao,Gong Ningqiang,Ma Chi,Zhang Yuxuan,Tan Hong,Qing Guangchao,Zhang Jimei,Wang Yufei,Wang Jinjin,Chen Shizhu,Li Xianlei,Ni Qiankun,Yuan Yuan,Gan Yaling,Chen Junge,Li Fangzhou,Zhang Jinchao,Ou Caiwen,Zhao Yongxiang,Liu Xiaoxuan,Liang Xing-Jie
Immunological adjuvants are essential for successful cancer vaccination. However, traditional adjuvants have some limitations, such as lack of controllability and induction of systemic toxicity, which restrict their broad application. Here, we present a light-activable immunological adjuvant (LIA), which is composed of a hypoxia-responsive amphiphilic dendrimer nanoparticle loaded with chlorin e6. Under irradiation with near-infrared light, the LIA not only induces tumour cell lysis and tumour antigen release, but also promotes the structural transformation of 2-nitroimidazole containing dendrimer to 2-aminoimidazole containing dendrimer which can activate dendritic cells via the Toll-like receptor 7-mediated signaling pathway. The LIA efficiently inhibits both primary and abscopal tumour growth and induces strong antigen-specific immune memory effect to prevent tumour metastasis and recurrence in vivo. Furthermore, LIA localizes the immunological adjuvant effect at the tumour site. We demonstrate this light-activable immunological adjuvant offers a safe and potent platform for in situ cancer vaccination.
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高影响因子
Communications Biology
·
2025
·
IF 5.1
Bai Xianan,Cai Shanshan,Jiang Jie,Tian Maojin,Du Jianxin,Zhao Peiqing
Breast cancer remains a leading cause of cancer-related mortality worldwide, largely due to the persistence of an immunosuppressive tumor microenvironment that limits therapeutic efficacy. However, the molecular mechanisms underlying this immune suppression are not fully understood. Here, we identify Semaphorin 7 A (Sema7a) as a key regulator of antitumor immunity in breast cancer through its interaction with Lysine Demethylase 4 A (Kdm4a). Transcriptomic analyses of The Cancer Genome Atlas (TCGA) dataset revealed Sema7a as an immune-related hub gene using Weighted Gene Co-expression Network Analysis and Least Absolute Shrinkage and Selection Operator algorithms. Functional experiments in breast cancer cell lines demonstrated that loss of Sema7a reduced Kdm4a expression, induced DNA replication stress, and activated the cGAS-STING signaling pathway, thereby increasing IFN-β and CXCL10 secretion. These changes enhanced CD8⁺ T cell chemotaxis and cytotoxic activity, suppressing tumor growth and metastasis in vivo. Conversely, Kdm4a overexpression reversed the antitumor effects of Sema7a deficiency. Our findings establish the Sema7a - Kdm4a axis as a crucial mechanism shaping the immunosuppressive microenvironment in breast cancer and highlight its potential as a therapeutic target to enhance antitumor immunity.
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Tingmin Pan,Jiuhui Ye,Jiening Li,Kuo Gui,Jian Li,Juntao Feng,Zhiqing Ma,Peng Lei,Yanqing Gao
Terpene-derived quaternary ring compounds with an oxime moiety were designed and prepared to create fungicides from natural products. A preliminary assessment of their antifungal activity against seven common pathogenic fungi was conducted, and the median effective concentration (EC50) values against Rhizoctonia solani were obtained. The effects of compound 6a19 (3-bromothiophene-containing), which had an outstanding EC50 value (1.62 μg/mL), on the morphology, ultrastructure, reactive oxygen species production, mitochondrial membrane potential, nuclear morphology, and defense-related and respiration-related enzyme activities of mycelia were evaluated. The test compound was speculated to obstruct the bio-oxidative process, inhibiting mycelial growth. Compound 6a19 exhibited a satisfactory in vivo control effect on leaf sheath-infected rice plants. After treating rice plants with 50, 100, and 200 μg/mL 6a19, the protective and therapeutic efficacy values were 48.3 and 70.3%, 58.6 and 75.7%, and 69.0 and 81.1%, respectively. Moreover, a linear quantitative structure–activity relationship (R2 = 0.932, F = 61.3, and S2 = 0.020) was established using density functional theory calculations. Four chemical descriptors that were crucial to the antifungal activity were analyzed: the number of occupied electronic levels of atoms, the minimum atomic orbital electronic population, maximum net atom charge for a H atom, and minimum net atomic charge. In overall consideration of experimental results, it was speculated that the target compounds satisfactorily inhibited R. solani by interfering with biological oxidation pathways, which provided an insight into the future intensive and systematic action mechanism. This research is promising for the invention of novel fungicides from natural terpenes with multiple potential targets and satisfactory ecological compatibility.
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FOOD AND AGRICULTURAL IMMUNOLOGY
·
2017
·
IF 1.392
Liqiang Liu,Steven Suryoprabowo,Qiankun Zheng,Shanshan Song,Hua Kuang
An immunochromatographic strip was developed for detection of acetamiprid (AC) and cross-reactivity with thiacloprid (TC) in cucumber and apple samples. This detection system was developed based on a sensitive and specific monoclonal antibody produced in our laboratory. Under optimized conditions, the cut-off limits of the test strip for AC were found to be 1 and 0.25 ng/mL for TC in phosphate-buffered saline. Meanwhile, with simple preparation for detection cucumber and apple samples, the cut-off limits for AC were found to be 5 and 2.5 ng/mL for TC in cucumber samples. The immunochromatographic assay also revealed AC cut-off values of 30 and 15 ng/mL in apple samples. Each test can be evaluated within 5 min. The data indicate that the method is sensitive, rapid, specific, and has the advantages of simplicity; therefore, this immunochromatographic assay is a useful screening method for detection of AC and TC residues in cucumber and apple samples.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2021
·
IF 8.128
Hanli Xing,Xiaofang Li,Susu Zhang,Jun Li,Baokun Tang
It is well known that almost all photoluminescent molecules are aromatic or heterocyclic ring compounds for bioimaging analysis. A question remains as to whether a breakthrough can be achieved regarding a novel photoluminescent molecule without a ring structure, and in a what manner. In this study, we explored the photoelectric conversion and structure of photoluminescent compounds, and constructed an intra-molecular coupling positive–negative-junction (P N J) with an open-loop and rigid Π 5 6 delocalized conjugated structure of the coupling p-π conjugate system. This was performed to enable strong absorption of the R/tail-end band for the high probability of an n → π*/n → σ* electron transition for photoluminescence production. Subsequently, the Π 5 6 structure was formed in a short-chain aliphatic molecule as a hydrolytic product of citric acid and urea, and computational methodology was employed to estimate the feasibility of the molecule photoluminescence. Finally, a quantum dot material was fabricated from the aliphatic molecule, the optical properties of the quantum dots were investigated, and the biocompatibility and bioimaging ability of quantum dots were assessed. This work presents not only a theoretical exploration but also practical application of a new strategy to obtain molecules, compounds, and materials with bioimaging.
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高影响因子
JOURNAL OF THE AMERICAN CERAMIC SOCIETY
·
2025
·Vol. 109
·期 1
·e70457
·
IF 3.8
Cong Ao,Qingqing Yang,Qingyang Shen,Zhehui Sun,Zhigang Peng,Qinghuang Zhang,Xiaofeng Zhang,Jun Xie,Dehua Xiong
Photovoltaic highly reflective coatings present a cost-effective strategy for enhancing solar module efficiency by facilitating secondary light absorption. However, developing a photovoltaic coating that simultaneously satisfies manufacturability, application suitability, and ultrahigh reflectivity remains a formidable challenge. In this study, we developed a novel Bi 2 O 3 –B 2 O 3 –ZnO–SiO 2 system glass formulated as photovoltaic ink for preparing a highly reflective coating. The glass components were designed and optimized via exploring the crystallinity and transmittance characteristics, yielding a 4%–5% increase in the reflectance of the coating. Furthermore, this study provides the first comprehensive analysis of the mechanisms governing the influence of several key factors—including glass fluidity, coating thickness, titanium dioxide content, and particle morphology—on coating reflectance. Through systematic optimization of coating composition and microstructure, an exceptional reflectance of 92.41% was achieved. These research findings represent a significant advance in the performance of highly reflective coatings and provide design principles for reflective composites in energy applications.
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JOURNAL OF BIOSCIENCES
·
2020
·Vol. 45
·期 1
·1-7
·
IF 1.645
Jin Jia-Yuan,Chen Ye,Wang Xing-You,Li Chen-Ming,Chen Wei-Lin,Li Li
Herein, we found that serum concentration of superoxide dismutase 3 (SOD3) was significantly reduced in children with mycoplasma pneumonia (MP) infection. To study the roles of SOD3 in inflammatory regulation of MP infection, human A549 type II alveolar epithelial cells were stimulated with 10 7 CCU/ml of MP to build MP infection in vitro . Secretion of pro-inflammatory cytokine interleukin (IL)-8 and tumor necrosis factor (TNF)-α were measured via enzyme-linked immunosorbent assay (ELISA) to assess the inflammatory response of A549 cells. Levofloxacin (LVFX) was used as an anti-inflammatory drug while recombinant TNF-α was used as an inflammatory promotor in MP-infected cells. Transcriptional activity of nuclear factor (NF)-кB was assessed by detecting protein levels of nuclear NF-кB and cytoplasm NF-кB using Western blot analysis. Our data suggested that the expression of SOD3 mRNA and protein, as well as content of SOD3 in cultured supernatant, were time-dependently inhibited in MP-infected A549 cells. However, lentiviruses-mediated SOD3 overexpression alleviated inflammatory response of MP-infected A549 cells, and prevented the unclear translocation of NF-кB, as evidenced by obviously reducing the production of IL-8 and TNF-α in cell cultured supernatant, as well as decreasing nuclear NF-кB while increasing cytoplasm NF-кB.
Inspiringly, SOD3 overexpression induced anti-inflammatory effect and the inactivation of NF-кB was similar to that of 2 μg/ml of LVFX, but reversed by additional TNF-α treatment. Therefore, we can conclude that transcriptional activity of NF-κB was the underlying mechanism, by which SOD3 regulated inflammatory response in MP infection in vitro .
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ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Jianlun Hu,Kangkang Feng,Yiyang Cong,Xiaoyang Li,Yanjun Jiang,Xiaodan Jiao,Yurong Li,Yuqin Zhang,Xinying Dong,Weifei Lu,Zhi Ding,Hao Hong
Shikonin (Shik), a natural pigment, has received growing interest in various biomedical fields due to its anti-inflammatory, antitumor, antimicrobial, and antioxidant ability. However, some inherent characteristics of Shik, such as its virulence, low bioavailability, and poor solubility, have limited its biomedical applicability. Here, we reported a facile synthetic method to produce the Shik-iron (III) nanoparticles (Shik-Fe NPs), which could overcome these limitations of Shik. The synthesized Shik-Fe NPs possessed a uniform size range of 110 ± 10 nm, negative surface charges, good water dispersity, and high safety. Iron distributed uniformly inside Shik-Fe NPs, and iron constituted 20% of total mass in PEGylated Shik-Fe NPs. When interacting with activated macrophages, Shik-Fe NPs significantly reduced the level of cellular inflammatory factors, for example, iNOS, IL-1β, and TNF-α. Furthermore, the Shik-Fe NPs demonstrated synergistic anti-inflammation and anti-bacterial properties in vivo, since they could release Fe3+ and Shik to eradicate bacteria (Staphylococcus aureus and P. aeruginosa were used as model microbes here) during wound infections and provide full recovery for scald wounds. Collectively, the study established a dual-functional Shik-derived nanoplatform, which could be useful for the treatment of various inflammation-involved diseases.
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RSC Advances
·
2017
·Vol. 7
·期 65
·41017-41023
·
IF 3.108
Ying Zhou,Jing Jing Fu,Ying Shuai Liu,Yue Jun Kang,Chang Ming Li,Ling Yu
Paper is a permeable porous material composed of a solid network of fibers. It is cheap, abundant, disposable and recyclable and has self-powered fluid wicking properties that are useful in building analytical devices. Paper-based cell assays are still in their infancy compared with enzyme- and protein-based analyses. For the first time, we show the potential of rice paper (an organic paper specifically used in Chinese calligraphy) for building cell analysis platforms. Rice paper's solution wicking and surface characterizations prove that it has a similar chemical configuration as that of a standard Whatman filter paper. Moreover, lactate dehydrogenase (LDH) release assay and WST-1 cell growth assay show that rice paper has better cell-compatibility features and improved cell distribution. The cell anchors and spreads along the cellulose fiber of the rice paper, whereas the porous rice paper matrix provides a sufficient surface area for cell growth. Cell-based immunohistochemistry was conducted to measure the expression of O-linked N-acetylglucosamine (O-GlcNAc) protein on prostate cancer cell DU145. An enhanced colorimetric signal was observed from cells grown on rice paper-based cell culture platform than those grown on 2D culture dish. The feasibility of fabricating rice paper with both direct crafting and wax printing—as well as on-paper cell immunoassays for on-demand applications—confirms the potential of rice paper as a new substrate for building paper devices for cell biology studies.
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危险品化学品经营许可证（带存储）