JOURNAL OF HAZARDOUS MATERIALS
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2015
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IF 4.529
Yao Wang,Wentao Zhu,Jing Qiu,Xinru Wang,Ping Zhang,Jin Yan,Zhiqiang Zhou
In the present study, the enantioselective metabolism, cytotoxicity of hexaconazole and its influence on tryptophan metabolism in rat hepatocytes in vitro were investigated. Following the exposure of primary rat hepatocytes to rac -hexaconazole, the concentrations of its enantiomers in the media were determined by chiral high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS). The half-lives ( t 1/2 ) of (+)-hexaconazole and (−)-hexaconazole were 5.17 h and 19.80 h, respectively, indicating that the metabolic process was enantioselective with (−)-hexaconazole enrichment. Using the MTT method, the EC 50 values of rac -hexaconazole, (+)-hexaconazole and (−)-hexaconazole after 12 h of exposure were determined to be 71.62, 62.71 and 67.94 μM, respectively. Tryptophan metabolism was monitored using metabolomics profiling techniques. Hexaconazole and its enantiomers caused the down-regulation of tryptophan levels and the up-regulation of kynurenine (KYN) levels, suggesting a role for hexaconazole in the activation of the KYN pathway and providing information for the mechanism of its toxicity.
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Oncotarget
·
2016
·
IF 5.008
Hui-Yun Zhang,Wen-Qian Xu,Yuan-yuan Zheng,Emmanuel Omari-Siaw,Yuan Zhu,Xia Cao,Shan-Shan Tong,Jiang-nan Yu,Xi-ming Xu
Cardiac glycosides could increase intracellular Ca 2+ ion by inhibiting the Na + /K + ATPase to induce apoptosis in many tumor cells. However, narrow therapeutic index, poor tumor selectivity and severe cardiovascular toxicity hinder their applications in cancer treatment. To improve the safety profile and tumor targetablility of cardiac glycosides, we designed octreotide conjugated periplocymarin, a cardiac glycoside isolated from Cortex periplocae . The conjugate showed higher cytotoxicity on MCF-7 cells and HepG2 tumor cells (SSTRs overexpression) but much less toxicity in L-02 normal cells. Tissue distribution studies of the conjugate using H 22 tumor model in mice showed higher accumulation in tumor and lower distribution in heart and liver than periplocymarin. Furthermore, in vivo anticancer effects of the conjugate on mice bearing H 22 cancer xenografts confirmed enhanced anti-tumor efficacy and decreased systemic toxicity. Altogether, octreotide-conjugated periplocymarin demonstrated tumor selectivity and may be useful as a targeting agent to improve the safety profile of cardiac glycosides for cancer therapy.
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POLYMERS FOR ADVANCED TECHNOLOGIES
·
2025
·Vol. 36
·期 3
·e70136
·
IF 3.4
Ping Xie,Jing He,Yangjun Ou
Photothermal therapy is a promising adjunctive treatment technique requiring a nanoplatform with excellent biocompatibility, photothermal stability, and precise targeting capabilities. This study aims to develop such a nanoplatform by synthesizing two new manganese(II)-based coordination polymers. The polymers were created by integrating 1,4-bis(imidazol-1-yl)benzene with the ternary ligand tris( p -carboxyphenyl)phosphane oxide, resulting in the formulas {[Mn 3 (L) 2 (bib) 3 (H 2 O) 7 ]·12H 2 O} n (CP 1 ) and {[Mn(HL)(bib)]·H 2 O} n (CP 2 ). These structures, with large surface areas and porosity, enhance drug loading efficiency. Doxorubicin (DOX) was successfully incorporated into a biocompatible HA/CMCS hydrogel-CP composite (HA/CMCS-CPs@DOX), forming a robust nanotherapeutic platform. Under near-infrared light irradiation, these metal-hydrogel composites effectively induced localized hyperthermia, aiding ocular melanoma treatment. We evaluated the effect of HA/CMCS-CP 2 @DOX on the cell proliferation of the uveal melanoma cell line, and the regulatory effects of the system on lncRNA ARAP1-AS1/miR-149-3p/S100A4 were also explored.
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ANALYST
·
2024
·
IF 3.6
Xiuqin Sheng,Jiakai Chen,Jiahui Shao,Xiaoya Zhang,Bing Wang,Chuan-Fan Ding,Yinghua Yan
The analysis of protein phosphorylation and glycosylation is critical for investigating disease development. In this work, 1,2-epoxy-5-hexene and N,N-methylenebisacrylamide were polymerized with vinyl phosphate to produce a polymer (denoted as PVME), which contained a variety of hydrophilic groups. The material's hydrophilicity was further enhanced by a ring-opening reaction with cysteine (the product was denoted as Cys-PVEM). Finally, titanium sulfate was combined with Cys-PVME to form titanium-rich polymers (Cys-PVME-Ti4+) for the enrichment of phosphopeptides and glycopeptides. Cys-PVME-Ti4+ has a good sensitivity (0.02 fmol) and selectivity (1 : 1000) with a loading capacity of 62 mg g−1, recyclability (9 cycles), and a good recovery rate (101.6 ± 0.60%) for phosphopeptides, and good sensitivity (0.01 fmol μL−1), selectivity (1 : 2000), a loading capacity of 62.5 mg g−1, recyclability (9 cycles), and a good recovery rate (98.7 ± 1.2%) for glycopeptides. In addition, after enrichment with this material, 27 phosphopeptides with 14 phosphoproteins and 223 glycopeptides associated with 88 glycoproteins were captured from the serum of colorectal cancer patients, while 27 phosphopeptides associated with 14 phosphoproteins and 210 glycopeptides associated with 111 glycoproteins were also captured from the serum of a normal control. Gene ontology (GO) analysis revealed that complement activation, extracellular region, extracellular space, blood coagulation, the IgG immunoglobulin complex, and heparin binding were different between normal control and colorectal cancer, implying that related pathways are likely involved in colorectal cancer pathogenesis.
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DESALINATION
·
2024
·
IF 8.3
Jiapeng Wang,Hua-Bin Yuan,Yongxuan Xiang,Lili Xing,Xinpeng Chen,Yifeng Wang,Jiazhuang Chen,Guoqiang Chen,Tieling Xing
Oxygen evolution reaction (OER), as a half-reaction of water decomposition, has a high theoretical overpotential. Therefore, the development of electrocatalysts with high OER performance is favorable for electrolytic hydrogen production. In situ growth of nanomaterials on conductive substrates is an effective strategy for the preparation of electrocatalysts. In this work, we grew Ni/Co bimetallic metal-organic framework (MOF) on carbon cloth substrates and successfully constructed a robust NiCo 2 S 4 /FeS@CC electrocatalyst through a MOF derivatization strategy. This electrocatalyst can be used for efficient and robust OER performance. MOF-derived NiCo 2 S 4 /FeS has the advantage of a porous heterostructure and multicomponent with many active sites and faster charge transfer rate, while sulfur doping greatly improves the OER performance. The current density of this self-supported heterogeneous material in alkaline freshwater reaches up to 10 mA cm −2 with an overpotential of only 238 mV. NiCo 2 S 4 /FeS@CC exhibited good OER performance in alkaline brine with an overpotential of only 241 mV at 10 mA cm −2 . We speculate that this is due to the generation of a negatively charged SO 4 2− anionic layer on the catalyst surface during the electrooxidation process, which effectively avoids Cl − corrosion. And both conditions demonstrate excellent long time stability. Additionally, we succeeded in further reducing the onset potential and energy consumption by adding ethanol or methanol to the electrolyte. This work provides an effective method to improve the OER performance of MOF-derived transition metal electrocatalysts for hydrogen production from electrolytic water.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Gong Chen,Panyang Gu,Wenfang Wu,Yuan Yin,Liangyu Pan,Shu Huang,Wei Lin,Meichun Deng
Histone methylations play a crucial role in the development of neuropathic pain, and SET domain containing 2 (SETD2), a histone methyltransferase, serves as the sole tri-methylase known to catalyze H3K36me3 at the gene body. The N -methyl- d -aspartate receptor (NMDAR) is activated and mediates excitatory synaptic transmission in neuropathic pain. Nevertheless, the involvement of SETD2 in neuropathic pain and the specific regulatory mechanisms affecting NMDARs remain poorly understood. The expression levels of SETD2 were significantly decreased in the spinal cord and dorsal root ganglion (DRG) of rodents undergoing neuropathic pain induced by sciatic nerve chronic constrictive injury. Lentiviral shRNA-mediated SETD2 knockdown and conditional knockout in sensory neurons caused sustained NMDAR upregulation in DRG and spinal cord, which resulted in heightened neuronal excitability and increased pain hypersensitivity. SETD2 deficiency also led to reduced H3K36me3 deposition within the Grin1 (glutamate ionotropic receptor NMDA type subunit 1) gene body, thereby promoting aberrant transcription of the NMDARs subunit GluN1. The absence of SETD2 in the DRG potentiated neuronal excitability and increased presynaptic NMDAR activity in the spinal dorsal horn. Chromatin immunoprecipitation sequencing targeting H3K36me3 identified NFAT5 as a co-transcription factor in the transcriptional regulation of Grin1 . These findings highlight SETD2 as a key regulator in pain signal transmission and offered new perspectives on the development of analgesics through the targeted modulation of epigenetic mechanisms.
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Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Zhifeng Li,Haomiao Cao,Zhaoyi Yin,Xiaoyi Dong,Jinge Hao,Zhiguo Song,Zhengwen Yang,Jianbei Qiu,Yongjin Li
Harvesting near-infrared (NIR) light to address the energy crisis and environmental pollution remains a challenge. In this study, full-spectrum-driven photocatalysts were realized through the design of Er 3+ /Yb 3+ co-doped Bi 3 Ti 2 O 8 F nanoplates. The Bi 3 Ti 2 O 8 F: Yb 3+ , Er 3+ nanoplates, when excited at 980 nm, demonstrated nearly single-band red upconversion (UC) emission. Furthermore, the corresponding UC emission mechanism was elucidated through the relationship between pump power and the UC emission spectrum. The experimental results and DFT calculation analysis revealed that the Er 3+ dopant ions form an impurity energy level, which broadens the light photoresponse range and promotes the separation of electron-hole (e − /h + ) pairs. Consequently, Bi 3 Ti 2 O 8 F:Yb 3+ , Er 3+ nanoplates exhibit 1.42 times better photocatalytic degradation of ciprofloxacin (CIP) than Bi 3 Ti 2 O 8 F under full-spectrum light irradiation and 4.54 times better than Bi 3 Ti 2 O 8 F: Er 3+ under NIR light irradiation. The trapping experiments and EPR analysis reveal that •O 2 – and h + are the main active species during the photocatalytic reaction process. Thus, this work demonstrates that efficient bismuth-based broadband photocatalysts can be developed through the simultaneous application of UC emission and doping engineering.
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Agronomy-Basel
·
2024
·Vol. 14
·期 6
·1302
·
IF 3.3
Xianghan Cheng,Feifei Liu,Peng Song,Xiaolei Liu,Qin Liu,Taiji Kou
The changes in biomass (including yield), copper (Cu) concentration, and the accumulation of wheat (Triticum aestivum. L) in response to soil Cu pollution under nighttime warming had still not been explored. Hence, this study was carried out, and these variations were analyzed from a physiological perspective. Pot trials were performed at two levels of ambient temperatures (no-warming (NT) and average nighttime warming of 0.28 °C (WT)) and two levels of soil Cu concentrations (control check without Cu application (CK) and 100 mg/kg Cu application (Cu)). Soil was collected from the carbonate cinnamon soil region of central China. The warming effects of the passive nighttime warming system were prominent, and the average increment was 0.28 °C. Antioxidant enzyme activities were promoted by warming (p< 0.05) and Cu. The highest yield was achieved in NT-Cu, mainly attributed to relatively strong root activity and photosynthesis caused by supplemental Cu, but the Cu concentration in its grains was close to the threshold (10 mg/kg) for Cu concentration in foodstuff and could present a potential food safety risk. Though nighttime warming did not increase the total biomass and yield of wheat, it decreased the Cu accumulation of wheat grown in Cu-contaminated soil, especially in grains. Moreover, WT-CK and WT-Cu increased the Cu concentration in the roots and glumes and reduced the Cu concentration in grains by 13.09% and 55.84%, respectively, probably because of a lower transpiration rate. Among them, the Cu concentration of grains in WT-Cu was the lowest and significantly lower than other applications. Our findings reveal that nighttime warming has the potential to reduce the Cu risk of grains in wheat grown in the Cu-contaminated carbonate cinnamon soil region of central China and could then provide a theoretical reference for risk assessment of food quality for wheat subjected to dual stress from nighttime warming and Cu pollution.
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Fishes
·
2025
·Vol. 10
·期 3
·116
·
IF 2.4
Qikai Liu,Yang Liu,Yaqi Mao,Ru Li,Yujie Jiao,Jiali Lei,Fenzhen Zhang
The degradation of organic matter using TiO2nanotube photocatalytic technology is limited by the short lifetime and diffusion radius of the generated hydroxyl radicals, decreasing the removal efficiency. This study developed a chlorine radical-coupled photoelectrocatalytic system, significantly enhancing the performance of TiO2nanotube arrays in removing sulfonamide antibiotics (SAs) from cold-water aquaculture systems. The highest degradation rates were observed at 5 mM NaCl and 15 mM NaNO3. When SA concentrations were 0.1–10 mg/L, degradation efficiency decreased with higher initial concentrations. The best degradation was achieved at an initial pH of 3 for SA. Humic acid and sodium acetate, natural organic matter in the water column, served as low-concentration promoters and high-concentration inhibitors. In our study, three degradation intermediates were identified, and hydrolysis and nitration reactions are proposed as the primary pathways for SA degradation. We confirmed that oxygen radicals play a major role in this system. Furthermore, toxicology experiments revealed the weakening of the toxicity of the degraded products. This study provides an efficient method for treating organic matter in cold-water fish culture water in chloride-containing saline and alkaline waters.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 9
·1-11
·
IF 5.3
Lu Juan,Guo Yanjia,Shan Xiangyu,Song Yujia,Li Ruidan,Tian Li,Li Xuwen
A solid-state electrochemiluminescence (ECL) sensor was fabricated by immobilizing luminol, a classical luminescent reagent, on a Zn-Co-ZIF carbon fiber-modified electrode for the rapid and sensitive detection of procymidone (PCM) in vegetable samples. The sensor was created by sequentially modifying the glassy carbon electrode with Zn-Co-ZIF carbon fiber (Zn-Co-ZIF CNFs), Pt@Au NPs, and luminol. Zn-Co-ZIF CNFs, prepared through electrospinning and high-temperature pyrolysis, possessed a large specific surface area and porosity, making it suitable as carrier and electron transfer accelerator in the system. Pt@Au NPs demonstrated excellent catalytic activity, effectively enhancing the generation of active substances. The ECL signal was significantly amplified by the combination of Zn-Co-ZIF CNFs and Pt@Au NPs, which can subsequently be diminished by procymidone. The ECL intensity decreased proportionally with the addition of procymidone, displaying a linear relationship within the concentration range 1.0 × 10 –13 to 1.0 × 10 –6 mol L −1 (R 2 = 0.993). The sensor exhibited a detection limit of 3.3 × 10 –14 mol L −1 (S/N = 3) and demonstrated outstanding reproducibility and stability, making it well-suited for the detection of procymidone in vegetable samples. Graphical Abstract
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Chuanbo Ding,Jiali Yang,Ning Wang,Qiteng Ding,Shuwen Sun,Yang Gao,Liqian Shen,Ting Zhao,Yue Wang
Diabetic wounds are typically chronic wounds and the healing process is limited by problems such as high blood glucose levels, bacterial infections, and other issues that make wound healing difficult. Designing drug-loaded wound dressings is an effective way to promote diabetic wound healing. In this study, we developed an SA/PVA nanofiber (SPS) containing Shikonin (SK) for the treatment of diabetic wounds. The prepared nanofibers were uniform in diameter, had good hydrophilicity and high water vapor permeability, and effectively promoted gas exchange between the wound site and the outside world. The results of in vitro experiments showed that SPS was effective in antimicrobial, antioxidant, and biocompatible. In vivo tests showed that the wound healing rate of mice treated with SPS reached 85.5 %. Immunohistochemical staining results showed that SPS was involved in the diabetic wound healing process through the up-regulation of growth factors (CD31, HIF-1α) and the down-regulation of inflammatory factors (CD68). Western blotting experiments showed that SPS attenuated the inflammation through the inhibition of the IκBα/NF-κB signaling pathway. These results suggest that SPS is a promising candidate for future clinical application of chronic wound dressings.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Zhen-Long Wang,Ke-An Wang,Xuechun Xiao,Hai-Bin Zhu
Amorphous and crystalline catalysts both have their own advantages to reduce the overpotential of OER. However, there is limited research on the relationship between the OER catalytic performance and the crystallinity degree. Herein, this key issue was explored by preparing FeW oxides with varying degrees of crystallinity. DFT combined with practical experimental results indicate that the change in crystalline state not only affects the electronic interactions in Fe-W-O but also the M-O bond lengths, both influencing the spin state of FeW oxides. At the critical temperature of amorphous-to-crystalline transition, a low-spin state of Fe in FeWO-450 is found to optimize the adsorption of intermediates during OER. FeWO-450 exhibits even better performance than commercial noble metal catalysts and numerous catalysts reported in the literature. This work showcases the effect of crystallinity of metal oxides on electron spin and offer a guidance how to improve OER properties by altering the electron spin states.
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危险品化学品经营许可证（带存储）