ENVIRONMENTAL POLLUTION
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2015
·
IF 4.143
Hai-Ming Zhao,Huan Du,Lei Xiang,Yan-Wen Li,Hui Li,Quan-Ying Cai,Ce-Hui Mo,Gang Cao,Ming-Hung Wong
To increase understanding on the mechanisms of cultivar difference in contaminant accumulation in crops, this study was designed to compare the physiological responses to di- n -butyl phthalate (DBP) exposure between low ( Lvbao70 ) and high ( Huaguan ) DBP cultivars of Chinese flowering cabbage ( Brassica parachinensis L.). Under high DBP exposure, significant differences in various physiological responses were observed between the two cultivars, which might account for the variation in DBP accumulation. Ultrastructure observation also showed different alterations or damages in the mesophyll cell structures between both cultivars, especially for the chloroplast disintegration, starch grain quantity, and plastoglobuli accumulation. Compared with Huaguan , Lvbao70 suffered greater decreases in biomass, chlorophyll content, carbon assimilation, gas exchange parameters, photosynthetic electron transport capacity, and antioxidase activities, which would have resulted in a great reduction of photosynthetic capacity. Although Lvbao70 enhanced energy dissipation and activities of some antioxidant enzymes, they did not provide sufficient protection against oxidative damage caused by DBP. The result suggested that the lower DBP tolerance of Lvbao70 might be associated with its poor physiological performances, which was responsible for its lower DBP accumulation to protect itself from toxicity. Additionally, Lvbao70 had a significantly lower transpiration rate and stomatal conductance than Huaguan , which might be the factors regulating DBP-accumulation variation.
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2015
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IF 4.152
Chune Zhu,Ying Huang,Xiaoying Zhang,Liling Mei,Xin Pan,Ge Li,Chuanbin Wu
The purpose of this study was to compare the properties of exenatide-loaded poly ( d , l -lactic-co-glycolic acid) microparticles (Ex-PLGA-MPs) prepared by a novel ultra-fine particle processing system (UPPS) and spray drying. UPPS is a proprietary technology developed by our group based on the disk rotation principle. Characteristics of the MPs including morphology, particle size distribution, drug content, encapsulation efficiency and in vitro release were comparatively studied. Cytotoxicity of the MPs was examined on A549 cells and the pharmacodynamics was investigated in vivo in type 2 diabetes Sprague-Dawley (SD) rats. Ex-PLGA-MPs prepared by UPPS showed larger particle size, denser surface, greater encapsulation efficiency, less initial burst release, and stable sustained release for more than one month in vitro as compared with the spray drying MPs. Meanwhile, the UPPS MPs effectively controlled the body growth rate and blood glucose in diabetes rats for at least three weeks after a single injection, while the spray drying MPs showed effective control period of about two weeks. UPPS technology was demonstrated to manufacture Ex-PLGA-MPs as a potential sustained release protein/polypeptide delivery system, which is an alternative method for the most commonly used spray drying. This comparative research provides a new guidance for microparticle preparation technology.
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POLYMER BULLETIN
·
2015
·Vol. 73
·期 1
·97-112
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IF 1.438
Teng Lijing,Nie Wangyan,Zhou Yifeng,Chen Pengpeng
In this paper, a series of star-shaped poly( l -lactide)s (s-PLLA) were synthesized via ring-opening polymerization of l -lactide (LLA) using erythritol as initiator. The structure and properties of s-PLLA were characterized by 1 H NMR, GPC and DSC. Then taking rifampicin (RIF) as the model drug, RIF-loaded s-PLLA microspheres were prepared using an oil-in-water emulsion solvent evaporation method. SEM and size analysis indicated that s-PLLA with high monomer/initiator molar ratios (s-PLLA98 and s-PLLA99) could form regular microspheres with smooth surface and have narrow size distributions, which hold the drugs well. The XRD spectra of the RIF-loaded microspheres demonstrated that s-PLLA and RIF were both in their amorphous state after the encapsulation process, which might be caused by the impediment effect of each other. The in vitro release profiles of the RIF-loaded microspheres showed that the s-PLLA99 microspheres were able to sustain the release of RIF for a considerable period of time (70–80 % within 180 h) and the release profiles of RIF from s-PLLA microspheres followed the Baker–Lonsdale model equation. Therefore, these biodegradable and biocompatible s-PLLA microspheres may find practical applications as drug delivery carriers.
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FOOD CHEMISTRY
·
2024
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IF 8.5
Ziwen Wang,Dongdong Wang,Jiaxuan Fang,Zixin Song,Jiman Geng,Jianfei Zhao,Yifan Fang,Changtao Wang,Meng Li
In this study, an ultrasonic-assisted natural deep eutectic solvent (NaDES) was used to extract flavonoids from Perilla frutescens (L.) Britt. leaves. Of 10 tested NaDESs, that comprising D-(+)-glucose and glycerol exhibited the best total flavonoid extraction rate. Response surface methodology (RSM) was used for extraction modeling and optimization, and the total flavonoid content reached 87.48 ± 1.61 mg RE/g DW, which was a significant increase of 5.36% compared with that of 80% ethanol extraction. Morphological changes in P. frutescens leaves before and after extraction were analyzed by scanning electron microscopy (SEM), and the mechanism of NaDES formation was studied by Fourier transform infrared (FT-IR) spectroscopy. Furthermore, 10 flavonoids were identified by UPLC-Q-TOF-MS. In addition, the NaDES extract had better biological activity according to several antioxidant, cyclooxygenase-2 (COX-2) and hyaluronidase (Hyal) inhibition experiments. Moreover, the stability test revealed that the total flavonoid loss rate of the NaDES extract after four weeks was 37.75% lower than that of the ethanol extract. These results indicate that the NaDES can effectively extract flavonoids from P. frutescens leaves and provide a reference for further applications in the food, medicine, health product and cosmetic industries.
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Journal of Fungi
·
2024
·Vol. 10
·期 11
·801
·
IF 4.2
Di Wang,Lingkui Meng,Haijue Zhang,Rong Liu,Yuhan Zhu,Xinyu Tan,Yan Wu,Qingchao Gao,Xueyan Ren,Qingjun Kong
Black spot, one of the major diseases of kiwifruit, is caused byAlternaria alternata. A comprehensive investigation into its pathogenicity mechanism is imperative in order to propose a targeted and effective control strategy. The effect of L-arginine on the pathogenicity ofA. alternataand the underlying mechanisms were investigated. The results showed that treatment with 5 mM L−1of L-arginine promoted spore germination and increased the colony diameter and lesion diameter ofA. alternatain vivo and in vitro, which were 23.1% and 9.3% higher than that of the control, respectively. Exogenous L-arginine treatment also induced endogenous L-arginine and nitric oxide (NO) accumulation by activating nitric oxide synthase (NOS), arginine decarboxylase (ADC) and ornithine decarboxylase (ODC). In addition, exogenous L-arginine triggered an increase in reactive oxygen species (ROS) levels by activating the activity and inducing gene expression upregulation of NADPH oxidase. The hydrogen peroxide (H2O2) and superoxide anion (O2.−) levels were 15.9% and 2.2 times higher, respectively, than in the control group on the second day of L-arginine treatment. Meanwhile, antioxidant enzyme activities and gene expression levels were enhanced, including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), glutathione peroxidase (GPX), and glutathione reductase (GR). In addition, exogenous L-arginine stimulated cell wall-degrading enzymes in vivo and in vitro by activating gene expression. These results suggested that exogenous L-arginine promoted the pathogenicity ofA. alternataby inducing the accumulation of polyamines, NO, and ROS, and by activating systems of antioxidants and cell wall-degrading enzymes. The present study not only revealed the mechanism by which low concentrations of L-arginine increase the pathogenicity ofA. alternata, but also provided a theoretical basis for the exclusive and precise targeting ofA. alternatain kiwifruit.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Yiwen Guo,Zitao Nie,Minjie Cao,Tian Yang,Guanjun Tao,Lijun Song,Ruijie Liu,Ming Chang,Xingguo Wang
Chimonanthus praecox (L.) Link kernel oil (LMO) has the potential to expand the variety of nutraceutical plant oils available and provide support for the application of functional food. This study aimed to assess the edible potential of LMO by examining its physicochemical characteristics, digestion behaviors, and nutraceutical properties. The results revealed that LMO has a high oil content of 40.84% and is particularly rich in linoleic acid (53.37–56.30%), oleic acid (22.04–25.08%) and triacylglycerol (TAG) of linoleic acid -palmitoleic acid- oleic acid (10.57–12.70%). The quality characteristics and phytochemical composition of LMO were found to be influenced by variety and extraction methods used. In simulated in vitro digestion tests, LMO showed a better lipid release rate and degree. Animal studies further demonstrated that LMO led to better TAG and cholesterol excretion compared to soybean oil and camellia oleifera oil. Overall, this study highlights the potential of LMO as a high-quality edible oil .
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Zongwei Hao,Ailong Hu,Juntao Cheng,Zhenni Ma,Zhaofeng Li,Jiali Lv,Huajian Xu,Huifang Ge,Hongyan Wang,Zhenyu Yu,Zhongwen Xie,Yiqun Du
The digestibility of starch-based foods is receiving increased attention. To date, the full understanding of how including L-theanine (THE) can modify the structural and digestive properties of starch has not been fully achieved. Here, we investigated the multi-scale structure and digestibility of maize starch (MS) regulated by THE in ultrasound field and the molecular interactions . Ultrasound disrupted the structure of starch granules and opened the molecular chains of starch, promoting increased THE binding and producing more low-order or disordered crystal structures. In this case, the aggregation of starch molecules, especially amylose , was reduced, leading to increased mobility of the systems. As a result, the apparent viscosity, G', and G" were significantly decreased, which retarded the starch regeneration. Density functional theory calculations indicated that there were mainly non-covalent interactions between THE and MS, such as hydrogen bonding and van der Waals forces. These interactions were the main factors contributing to the decrease in the short-range ordering, the helical structure, and the enthalpy change (ΔH) of MS. Interestingly, the rapidly digestible starch (RDS) content of THE modified MS (MS-THE-30) decreased by 17.89 %, while the resistant starch increased to 26.65 %. These results provide new strategies for the safe production of resistant starch.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Xiaoxiao Liu,Kai Li,Luona Ye,Xinghong Cao,Pengbo Wang,Xiaoman Xie,Min Yang,Li Xu,Yunjun Yan,Jinyong Yan
We propose a co-immobilized chemo-enzyme cascade system to mitigate random intermediate diffusion from the mixture of individual immobilized catalysts and achieve a one-pot reaction of multi-enzyme and reductant. Catalyzed by lipase and lipoxygenase, unsaturated lipid hydroperoxides (HPOs) were synthesized. 13( S )-hydroperoxy-9Z, 11E-octadecadienoic acid (13-HPODE), one compound of HPOs, was subsequently reduced to 13( S )-hydroxy-9Z, 11E-octadecadienoic acid (13-HODE) by cysteine. Upon the optimized conditions, 75.28 mg of 13-HPODE and 4.01 mg of 13-HODE were produced from per milliliter of oil. The co-immobilized catalysts exhibited improved yield compared to the mixture of individually immobilized catalysts. Moreover, it demonstrated satisfactory durability and recyclability, maintaining a relative HPOs yield of 78.5% after 5 cycles. This work has achieved the co-immobilization of lipase, lipoxygenase and the reductant cysteine for the first time, successfully applying it to the conversion of soybean oil into 13-HODE. It offers a technological platform for transforming various oils into high-value products.
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JOURNAL OF FOOD ENGINEERING
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2024
·
IF 5.3
Obafemi Ibitayo Obajemihi,Jun-Hu Cheng,Da-Wen Sun
The effect of plasma functionalized water (PW) pretreatment and influencing factors including treatment time and voltage were investigated on the drying kinetics, nutrition and sensory properties of tomato slices during infrared-accelerated pulsed vacuum drying (IR-PVD). Results revealed that PW pretreatment caused disruptions in tomato cellular structure that were greater with increasing treatment time and voltage, due to strong acidification and etching from reactive species in PW. These disruptions accelerated the removal of moisture during IR-PVD to shorten drying time by 25%, and improve visual appearance, rehydration, porosity, phenols and flavonoids, soluble solids, lycopene, ascorbic acid, but reduced chewiness, gumminess and hardness properties. Overall, the findings revealed the propitious potentials of PW and indicated the significant effects of PW influencing factors for accelerating drying characteristics and increasing the functional and nutrition properties of tomato slices during IR-PVD, providing a theoretical basis for developing novel energy-saving drying approaches.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Junbo Chu,Songyi Lin,Yi Yuan,Siqi Zhang,Simin Zhang
Eel is a commercially important marine fish, frequently featured as sushi or roasted preparations. This study determined the formation of heterocyclic amines (HAs) and advanced glycation end products (AGEs) in roasted eel and evaluated the inhibitory mechanism of quercetin and l -ascorbic acid on their formation. The results indicate a respective reduction of 75.07% and 84.72% in total HAs, alongside a decline of 23.03% and 39.14% in AGEs . Additionally, fundamental parameters of roasted eel, lipid oxidation indicators and precursors were measured to elucidate the mechanisms and impact of natural antioxidants on HAs and AGEs formation in roasted eel. Furthermore, endeavors were made to probe into the molecular mechanisms governing the influence of key differential lipids on the generation of HAs and AGEs through lipid-mics analysis. This research emphasizes the potential of natural antioxidants in preventing harmful substances formation during eel thermal processing, which is helpful to food manufacturers for healthier food production.
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JOURNAL OF POWER SOURCES
·
2024
·
IF 8.1
Chenhan Xiong,Qiang Long,Jiaqi Chen,Yanqiu Yu,Xinming Lian,Rui Xue,Junjie Wang,Shufang Zheng,Guoping Du,Nan Chen
Effective design and construction of highly electrochemically active materials on conductive substrates with hierarchical nanostructures are critical for enhancing the electrochemical energy storage performance of electrodes. However, the design of such special structures is still challenging. In this study, a special three-dimensional@three-dimensional (3D@3D) NiCo-layered double hydroxide (NiCo-LDH) composite nanostructure is developed through the innovative approach of in-situ growth of arrayed Co-Zif-L on conductive substrate, disrupting the Zif-67 formation. This is followed by ion exchange process and electrochemical synthesis for growing 3D NiCo-LDH nanosheets. A unique structure with the 3D NiCo-LDH embedded in the 3D Co-Zif-L structure, termed as 3D@3D, is resulted. This spatial structure not only enhances their mechanical stability and adhesion strength through the in-situ growth process, but also provides a greater number of electrochemical active sites due to its ultra-large specific surface area. Density functional theory (DFT) calculations reveal that the NiCo-LDH@Co-Zif-L composite nanostructure exhibits an enhanced density of states (DOS) near the Fermi level compared to individual components, indicating excellent conductivity. With this unique structure, the NiCo-LDH@Co-Zif-L/NF electrode demonstrates an area-specific capacity of 4.1C cm −2 and retains 81.9 % of its initial capacitance after 5000 cycles. Moreover, the assembled hybrid supercapacitor achieves an energy density of 222.2 mWh cm −2 at a power density of 800 mW cm −2 (66.9 Wh kg −1 at 245 W/kg). The innovative approach in this work provides new insights into the utilization of Zif materials and designing electrode materials with special structures for high-performance electrochemical energy storage devices.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Xintian Wang,Hengye Chen,Yao Fan,Wei Lan,Juan He,Haiyan Fu,Xiaolong Yang
The economic and medicinal value of Atractylodis Rhizoma (AR) is intricately tied to its geographical origin and mainly depends on the atractylodin (IUPAC Name: 2-[(1E,7E)-nona-1,7-dien-3,5-diynyl]furan) content in it. In this study, a green fluorescent sensor using N-acetyl-L-cysteine (NAC, IUPAC Name: (2R)-2-acetamido-3-sulfanylpropanoic acid)-capped CdTe quantum dots (QDs) was developed for the detection of AR origin discrimination. Both electron transfer and internal filtration effects was introduced into sensor to improve the sensitivity and selectivity. The sensors can achieve visual quantitative analysis of atractylodin using the partial least squares regression (PLSR) model. This model with a detection limit of 0.321 µg /mL and the quantification range of 0.002–0.030 mg /mL. Furthermore, the visual sensor also could be applied to the quantification of atractylodin in the actual AR samples with recoveries as high as 95.36 % to 105 %. Their sensor was also able to identify different origin of AR with an accuracy of 100 % for the training set and 95.29 % for the prediction set using the Partial Least Squares Discriminant Analysis (PLSDA) model. The method offers direction for verifying the authenticity of traditional Chinese medicine and precise analysis of intended constituents in complex systems.
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