FOOD CONTROL
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2023
·
IF 6
Shiqi Hu,Xinglian Xu,Wangang Zhang,Chunbao Li,Guanghong Zhou
The activity of cathepsin B in Jinhua ham was affected by various processing parameters, which were investigated and quantified using a multi-layer artificial neural network (ANN). The key factors that influence ham quality are processing temperature, pH, and sodium chloride content. The study discussed the univariate analysis and multivariate interactions between two or three factors. The established ANN model was used for further investigation, and the actual enzyme activity of cathepsin B was characterized. The results showed that high temperature (greater than 60 °C) and an alkaline environment or the addition of Na + cation significantly reduced the enzyme activity ( p < 0.05). The optimal conditions for protease activity were found to be at 40 °C and pH 6.0. The enzyme was found to be unstable at a temperature of 20 °C or pH 6.5–7.0 in the presence of NaCl, indicating significant interactions between temperature, pH value and NaCl content ( p < 0.01). Furthermore, the ANN model showed good match between true and predicted values. The correlation coefficient was 0.98468, and the model provided supplementary information about the effect of NaCl on cathepsin B activity within 0.4M–0.6M. The findings could be useful in Jinhua ham production and the study of the structure-activity relationship of cathepsin B.
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高影响因子
SENSORS
·
2023
·Vol. 23
·期 14
·6277
·
IF 3.9
Ya Wang,Yuanhua Wei,Siqi Li,Guang Hu
Benefiting from the advantages like large surface area, flexible constitution, and diverse structure, metal-organic frameworks (MOFs) have been one of the most ideal candidates for nanozymes. In this study, a nitro-functionalized MOF, namely NO2-MIL-53(Cu), was synthesized. Multi-enzyme mimetic activities were discovered on this MOF, including peroxidase-like, oxidase-like, and laccase-like activity. Compared to the non-functional counterpart (MIL-53(Cu)), NO2-MIL-53(Cu) displayed superior enzyme mimetic activities, indicating a positive role of the nitro group in the MOF. Subsequently, the effects of reaction conditions on enzyme mimetic activities were investigated. Remarkably, NO2-MIL-53(Cu) exhibited excellent peroxidase-like activity even at neutral pH. Based on this finding, a simple colorimetric sensing platform was developed for the detection of H2O2and glucose, respectively. The detection liner range for H2O2is 1–800 μM with a detection limit of 0.69 μM. The detection liner range for glucose is linear range 0.5–300 μM with a detection limit of 2.6 μM. Therefore, this work not only provides an applicable colorimetric platform for glucose detection in a physiological environment, but also offers guidance for the rational design of efficient nanozymes with multi-enzyme mimetic activities.Keywords:nanozyme;metal-organic frameworks;multi-enzyme mimetics;glucose;colorimetric sensor
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FOOD HYDROCOLLOIDS
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2023
·
IF 10.7
Huishan Rao,Fei Chen,Lianzhu Lin,Mouming Zhao
Flavonoids have a variety of bioactivities, but their poor solubility, low bioavailability, and weak stability limit their application as functional factors in food systems. Soy extracts (SE) mainly composed of soy proteins and soluble polysaccharides at mass ratios of 4.1–5.8: 1 and possessing good bioactivity and nutritional property might be candidate functional encapsulation carriers to improve defects of flavonoids and enhance overall nutritional quality . In this study, galangin (Gal), a poorly water-soluble flavonoid, was used as an example to evaluate the application potential of SE in flavonoid delivery system. Gal-loaded SE nanoparticles (Gal-loaded SE NPs) with high Gal solubility, encapsulation efficiency and bioaccessibility were successfully constructed by pH-driven method. The solubilizing effect of SE on Gal largely depended on the concentrations of SE and Gal. Combining the encapsulation efficiency and retention rate, the optimal conditions were identified as SE concentration of 40 mg/mL, binding time of 5 min, and Gal to SE mass ratio of 1%. Gal-loaded SE NPs showed good storage, thermal, redissolution, and sodium ions stabilities. Based on Fourier transform infrared spectroscopy , X-ray diffraction, and transmission electron microscope analysis , the proteins in SE might undergo a typical de-folding and re-folding process during pH-shifting and mainly combine with Gal by hydrophobic interaction to form Gal-loaded SE NPs with spherical shape (particle diameter <100 nm). This study broadened the application of SE in flavonoid delivery system and provided new ideas for the design and development of new plant-based health food ingredients with multiple functional factors and high nutritional quality.
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Food Bioscience
·
2023
·
IF 5.2
Xianghui Kong,Weiqi Xu,Kaixin Zhang,Guijie Chen,Xiaoxiong Zeng
Theaflavins (TFs) and theasinensins (TSs) are the key components formed from the enzymatic oxidation of tea catechins , contributing significantly to the biological activities and taste of black and Oolong teas. In this study, we aimed to reveal the catechin conversion and the enzymatic synthesis of TFs and TSs under reaction temperatures and pH values using polyphenol oxidase (PPO) from potato tuber ( Solanum tuberosum L.). The results indicated that different temperatures, pH values and durations had significant effects on the conversion of catechins and the formation of TFs and TSs. During the reaction, the most of catechin substrates was consumed at 60–90 min after reaction initiation, and large amounts of the products TFs (0.04–0.41 mg/mL) and TSs (0.51–1.52 mg/mL) were obtained. The content of TSs was significantly higher and the maximum content of TSs was more than three times that of TFs. Reaction at a lower temperature (20 °C) is suitable for producing dimers, especially for TFs. Higher pH value resulted in higher catechin consumption and higher TSs content, while the maximum of TFs was obtained at pH 5.0. The present results provided a theoretical basis and practical guidance for the targeted processing of tea catechin dimers in vitro .
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ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Yukun Wang,Zhiyu Jin,Zijun Zhang,Changkai Zhou,Zhe Sun,Feng Yan
With the requirements for food safety and quality, there has been increasing attention on intelligent food packaging, especially pH-responsive intelligent packaging. However, the toxicity of indicators and the vulnerability of composite films to leakage tend to change the composition of food and endanger human health. In this study, 2-allyoxy-1-hydroxy-anthraquinone (AhAQ), a pH-responsive plant dye that was modified from alizarin (AI), was grafted onto the pH-responsive intelligent film (AhAQF) via click polymerization. The obtained AhAQF film shows color change in response to ammonia vapor and exhibits adequate reversibility after treatment with volatile acetic acid. The obtained AhAQF exhibits zero leakage, owing to the covalent immobilization of AhAQ. Thus, the prepared pH-responsive films are non-toxic and antibacterial and show promising application prospects in visual food intelligent packaging and gas-sensitive labels.
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PubMed
高影响因子
ANALYST
·
2023
·Vol. 148
·期 10
·2375-2386
·
IF 4.2
Yuwei Du,Lei Cao,Xinlu Li,Tongtong Zhu,Ruhong Yan,Wen-Fei Dong,Li Li
Carbon dots (CDs) with red fluorescence emission are highly desirable for use in bioimaging and trace- substance detection, with potential applications in biotherapy, photothermal therapy, and tumor visualization. Most CDs emit green or blue fluorescence, thus limiting their applicability. We report a novel fluorescent detection platform based on high-brightness red fluorescence emission carbon dots (R-CDs) co-doped with nitrogen and bromine, which exhibit pH and oxidized L-glutathione (GSSG) dual-responsive characteristics. The absolute quantum yield of the R-CDs was as high as 11.93%. We discovered that the R-CDs were able to detect acidic pH in live cells and zebrafish owing to protonation and deprotonation. In addition, GSSG was detected in vitro over a broad linear range (8–200 μM) using the R-CDs with excitation-independent emission. Furthermore, cell imaging and bioimaging experiments demonstrated that the R-CDs were highly cytocompatible and could be used as fluorescent probes to target lysosomes and nucleolus. These studies highlight the promising prospects of R-CDs as biosensing tools for bioimaging and trace-substance detection applications.
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CHEMOSPHERE
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2023
·
IF 8.8
Yang-xiao-xiao Shi,Jia-qi Cui,Feng Zhang,Ke-wei Li,Jun Jiang,Ren-kou Xu
Hexavalent chromium (Cr(VI)) is a toxic heavy metal and its mobility and bioaccessibility in soils are influenced by soil properties. In this study, the soil pH and organic carbon contents of Ultisol , Alfisol , and Inceptisol were adjusted before they were polluted with 230 mg kg −1 Cr(VI). Alkaline digestion, sequential extraction, and an in vitro experiment were conducted to study the valence state, species, and bioaccessibility of Cr in the soils. The results showed that a high soil pH was not favorable for reduction of Cr(VI); therefore the Cr(VI) and exchangeable Cr contents were positively related to soil pH. Soil organic carbon promoted the reduction of Cr(VI). Almost all Cr(VI) was reduced to Cr(III) when the soil organic carbon content reached 10 g kg −1 . Chromium bioaccessibility in simulated gastric and intestinal phase solutions was influenced by Cr(VI) and Cr(III) adsorption/desorption, dissolution/precipitation, and redox reactions. Chromium bioaccessibility differences between the gastric and intestinal phases were associated with the Cr(VI)/Cr(III) ratio. Acidic conditions and a high organic carbon content promoted the conversion of Cr(VI) to Cr(III). When soil pH was increased from 4.01 to 5.85, Cr(VI) in Alfisol without the addition of humic acid increased from 96.38 to 174.78 mg kg −1 , the exchangeable Cr proportion increased from 9.7% to 22.6%, and Cr bioaccessibility increased from 41.29% to 49.14% in the gastric phase and from 41.32% to 48.24% in the intestinal phase. When the organic carbon content increased from 3.95 to 9.28 g kg −1 in Alfisol, Cr(VI) content decreased from 167.66 to 20.52 mg kg −1 , which led to a decrease in Cr bioaccessibility from 49.15% to 13.8% in the gastric phase and from 45.85% to 7.67% in the intestinal phase. Therefore, acidic conditions and increasing soil organic carbon levels can reduce the health risk posed by Cr in soils.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Rui Wang,Man-Qin Zeng,Yu-Wei Wu,Yong-Xin Teng,Lang-Hong Wang,Jian Li,Fei-Yue Xu,Bo-Ru Chen,Zhong Han,Xin-An Zeng
In this study, soy protein isolate (SPI) was modified by a pulsed electric field (PEF) combined with pH shifting treatment (10 kV/cm, pH 11) to prepare SPI nanoparticles (PSPI11) for efficient loading of lutein. The results showed that when the mass ratio of SPI to lutein was 25:1, the encapsulation efficiency of lutein in PSPI11 increased from 54% to 77%, and the loading capacity increased by 41% compared to the original SPI. The formed SPI-lutein composite nanoparticles (PSPI11-LUTNPs) had smaller, more homogeneous sizes and larger negative charges than SPI7-LUTNPs. The combined treatment favored the unfolding of the SPI structure and could expose its interior hydrophobic groups to bind with lutein. Nanocomplexation with SPIs significantly improved the solubility and stability of lutein, with PSPI11 showing the greatest improvement. As a result, PEF combined with pH shifting pretreatment is an effective method for developing SPI nanoparticles loaded and protected with lutein.
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高影响因子
Acta Biomaterialia
·
2023
·
IF 9.7
Jie Zhang,Liwen Wei,Xiaocao Ma,Jingguo Wang,Siping Liang,Kang Chen,Minhao Wu,Li Niu,Yuanqing Zhang
Metabolic dysregulation contributes not only to cancer development but also to a tumor immune microenvironment (TIME), which poses great challenges to chemo- and immunotherapy. Targeting metabolic reprogramming has recently emerged as a promising strategy for cancer treatment, but the lethality against solid tumors appears to be fairly restricted, partially due to the poor solubility of small molecule drugs. Herein, we construct a versatile biomimetic nanoplatform (referred to as HM-BPT) employing pH-sensitive tumor-tropism hybrid membrane-coated Manganese oxide (MnO 2 ) nanoparticles for the delivery of BPTES, a glutamine metabolism inhibitor. Basically, hybrid membranes consisting of mesenchymal stem cell membranes (MSCm) and pH-sensitive liposomes (pSL) enable the biomimetic nanoplatform to target TME and escape from endo/lysosomes after endocytosis . The results reveal that HM-BPT treatment leads to remarkable tumor inhibition, cytotoxic T lymphocyte (CTL) infiltration , as well as M1 phenotype repolarization and stimulator of IFN genes (STING) pathway activation in macrophages in a 4T1 xenograft model. Furthermore, glutathione (GSH) depletion and oxygen (O 2 ) supply synergistically ameliorate the immunosuppressive status of the TME, boosting potent antitumor immune responses. Overall, our study explores an integrated therapeutic platform for TME reprogramming and immune activation, offering tremendous promise for cancer combination therapy. Statement of significance Metabolic abnormalities and the tumor immune microenvironment (TIME) lead to hyporesponsiveness to conventional therapies, ultimately resulting in refractory malignancies. In the current work, a biomimetic nanoplatform (HM-BPT) was developed for TME metabolic reprogramming in favor of immunotherapy. Particularly, hybrid membrane camouflage endowed the nanoplatform with TME targeting, endo/lysosomal escape, and sensitive release properties. The impact of hybrid membrane fusion ratio on cellular uptake and cell viability was explored, yielding beneficial references for the future development of bioactive nanomaterials . Intravenous administration of HM-BPT substantially relieved tumor burden and restored innate and acquired immune activation in 4T1 xenograft models. In conclusion, the created HM-BPT system has the potential to be a promising nanoplatform for combining cancer therapies.
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JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2023
·
IF 4.3
Rui Wang,Simin He,Wanjing Liu,Hebing Pei,Nijuan Liu,Ruibin Guo,Zunli Mo
Carbon quantum dots are widely used in the field of analysis and detection due to their excellent optical properties. At present, many studies have focused on the preparation of multi-color fluorescent quantum dots by simple methods. Inspired by this, carbon quantum dots with green (G-CQDs) and yellow fluorescence (YN-CQDs) emission were prepared by hydrothermal method using PTCDA and urea as precursors. After characterizing their morphology, structure, and optical properties, fluorescent probes for different purposes were constructed according to their different fluorescence characteristics. Since green G-CQDs are sensitive to acidic environments, pH detection kits are developed. In addition, G-CQDs can also efficiently detect Asp. YN-CQDs are used to detect metal ions and can specifically detect Fe 3+ . Both of them were applied to the detection of actual samples, showing high efficiency and reliability. It provides a new idea for the multi-purpose detection of carbon quantum dot fluorescent probes.
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高影响因子
Materials Today Communications
·
2023
·
IF 3.8
Zuokun Zhang,Sisi Zhou,Xingui Wang,Ruimei Liang,Xingxin Sheng,Yongbo Zhu,Lijun Huang,Binbin Zhou,Ming Zhong
Conventional self-assembled peptide hydrogels have poor mechanical properties and tend to release abruptly when they are used as drug carriers, limiting the application of these materials in biomedical field. In this work, gelatin was embedded in a pH-responsive peptide Ac-FALNLAKD-NH 2 (PEP) to construct gelatin/peptide composite hydrogels (Gel/PEP) for controlling the delivery of the anticancer drug chelerythrine (CHE). The physicochemical properties of Gel/PEP composite hydrogels were evaluated. The results of circular dichroism and Fourier transform infrared spectroscopy characterization showed that compared with PEP hydrogels and gelatin, the Gel/PEP composite hydrogel has a superposition of two secondary structures, in which β-sheet and random coil accounted for 44.7 % and 55.3 % of the conformation, respectively. Rheology and scanning electron microscope characterization confirmed that the Gel/PEP hydrogel has a highly dense nanofibrous meshwork structure, which enhances mechanical and injectable properties. In addition, the encapsulation rate of CHE could reach more than 97 % at a gelatin/peptide mass ratio of 7:1. More importantly, in vitro results showed that the composite hydrogel still had good pH and thermal response performance, which can achieve controlled release of CHE. Therefore, it is possible to synthesize supramolecular drug release soft materials by composite of gelatin and peptide hydrogels.
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高影响因子
Gels
·
2023
·Vol. 9
·期 6
·438
·
IF 4.6
Chao Ma,Shuyi Peng,Lian Chen,Xingyu Cao,Ye Sun,Lin Chen,Lang Yang,Chunming Ma,Qijie Liu,Zhenzhong Liu,Shaohua Jiang
Stimuli-responsive color-changing and shape-changing hydrogels are promising intelligent materials for visual detections and bio-inspired actuations, respectively. However, it is still an early stage to integrate the color-changing performance and shape-changing performance together to provide bi-functional synergistic biomimetic devices, which are difficult to design but will greatly expand further applications of intelligent hydrogels. Herein, we present an anisotropic bi-layer hydrogel by combining a pH-responsive rhodamine-B (RhB)-functionalized fluorescent hydrogel layer and a photothermal-responsive shape-changing melanin-added poly (N-isopropylacrylamide) (PNIPAM) hydrogel layer with fluorescent color-changing and shape-changing bi-functional synergy. This bi-layer hydrogel can obtain fast and complex actuations under irradiation with 808 nm near-infrared (NIR) light due to both the melanin-composited PNIPAM hydrogel with high efficiency of photothermal conversion and the anisotropic structure of this bi-hydrogel. Furthermore, the RhB-functionalized fluorescent hydrogel layer can provide rapid pH-responsive fluorescent color change, which can be integrated with NIR-responsive shape change to achieve bi-functional synergy. As a result, this bi-layer hydrogel can be designed using various biomimetic devices, which can show the actuating process in the dark for real-time tracking and even mimetic starfish to synchronously change both the color and shape. This work provides a new bi-layer hydrogel biomimetic actuator with color-changing and shape-changing bi-functional synergy, which will inspire new strategies for other intelligent composite materials and high-level biomimetic devices.
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高影响因子
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