TALANTA
·
2018
·
IF 4.244
Miaoduo Deng,Meng Li,Yu Zhao,Zhen Jiang,Xingjie Guo
With native β-cyclodextrin (β-CD) added into the polymerization mixture directly, a novel, convenient and low-cost one-pot strategy was developed to prepare the β-CD functionalized organic polymer monolithic capillary column. Diazabicyclo[5.4.0]undec-7-ene (DBU) as a basic catalyst for the ring opening reaction between β-CD and glycidyl methacrylate (GMA) was introduced into the polymerization system for the first time. Thereby, two consecutive reactions namely the in situ methacrylation of β-CD and copolymerization reaction can be achieved in one pot. The preparation conditions including the type and composition of porogens，the ratio of functional monomer to crosslinker and amount of 2-acrylamido-2-methyl propane sulfonic acid (AMPS) were optimized. The specific surface area and morphology of the prepared monolith were characterized using scanning electron microscopy (SEM) and nitrogen adsorption analysis, respectively. Raman spectroscopy and nuclear magnetic resonance (NMR) spectroscopy confirmed that β-CD was covalently bonded onto the monolith successfully. Then, the monolithic column was applied to enantioseparation of six basic drugs in capillary electrochromatography (CEC). Under the optimal conditions, tropicamide , homatropine , homatropine methylbromide, brompheniramine , chlorpheniramine and clorprenaline were all totally separated with the resolution (Rs) values of 2.84, 4.70, 4.61, 3.01, 2.57 and 2.33, respectively. Furthermore, the column demonstrated satisfactory stability and repeatability of retention time and enantioselectivity using homatropine as model analyte.
研究领域:
分析化学
·
药物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Applied Biological Chemistry
·
2024
·Vol. 67
·期 1
·1-11
·
IF 2.3
Yu Ziqing,He Wen,Shi Weiwu
All chronic renal disorders eventually lead to renal interstitial fibrosis (RIF). Chronic inflammation and pro-fibrotic substances are familiar companions of the fibrotic process. The Sulforaphane (Sul) molecule is particularly useful in protecting the liver from oxidative damage. To investigate the Sul effects on fibrosis markers and inflammatory proteins in the kidney of NRK52E cell line and rats and clarify the mechanism of TGF-β/Smad signaling pathway in a rat model of RIF were developed in the present study. Sul (50, 100, and 200 ng/ml) remarkably reduced the gene expressions of tumor necrosis factor (TNF-α), interleukin-6 (IL-6), interleukin (IL)-1β, collagen 3 (COL3A1), collagen 1 (COL1A1), and α-smooth muscle actin (α-SMA) in fibrotic NRK52E cells compared with those in cells inspired by transforming growth factor-α (TGF-α). Histopathological investigations showed that Sul administration retained renal tissue structure and decreased kidney tissue fibrosis in rats subjected to unilateral ureteral blockage (UUO). The expression level of TNF-α, IL-6, IL-1β, COL3A1, COL1A1, and α-SMA in the rats’ kidneys exposed to UUO was also suppressed by the treatment of Sul. In the present study, western blot analysis showed that Sul upregulated the expressions of fibrotic NRK52E cells Smad7 and rat model UUO groups while simultaneously decreasing the stimulation of Smad2/3 and the expressions of cyclooxygenase-2, NF-κB, Smad4, activator protein-1, and high-mobility group protein B1. Ultimately, Sul’s ability to inhibit the TGF-β/Smad pathway and the development of inflammation factors may mitigate RIF.
研究领域:
医学
·
药理学
·
分子生物学
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2018
·
IF 3.997
Conglian Yang,Yuting Qin,Kun Tu,Chenfeng Xu,Zhen Li,Zhiping Zhang
Multidrug resistance (MDR) remains as an obstacle for effective cancer treatment. Herein, we developed a novel and efficient nanomedicine by virtue of the carrier characters and MDR inhibition effects of β-cyclodextrin (β-CD) and d-α-tocopheryl polyethylene glycol succinate (TPGS). A series of star-shaped polymers CD-g-TPGS with different TPGS substitution degree were synthesized for doxorubicin (DOX) delivery, where β-CD was identified as a core and TPGS as branches. These star polymers can self-assemble into nanoparticles with DOX . These nanoparticles showed no significant differences in size, zeta potential and morphology except for in vitro stability. They demonstrated good biocompatibility and enhanced cellular uptake in both drug sensitive and resistant cancer cells. Notably, the nanoparticles exhibited superiority of cytotoxicity in drug resistant cancer cells against free DOX . In vivo antitumor effect also demonstrated the improved cancer inhibition effect. This work suggests that star-shaped polymers CD-g-TPGS are promising drug carriers to overcome MDR in cancer treatment.
研究领域:
药学
·
纳米技术
·
癌症研究
✓ 已验证
DOI
高影响因子
BRAIN
·
2021
·Vol. 144
·期 9
·2759-2770
·
IF 13.501
Zhang Yalun,Zhang Yi,Aman Yahyah,Ng Cheung Toa,Chau Wing-Hin,Zhang Zhigang,Yue Ming,Bohm Christopher,Jia Yizhen,Li Siwen,Yuan Qiuju,Griffin Jennifer,Chiu Kin,Wong Dana S M,Wang Binbin,Jin Dongyan,Rogaeva Ekaterina,Fraser Paul E,Fang Evandro F,St George-Hyslop Peter,Song You-Qiang
The molecular link between amyloid-β plaques and neurofibrillary tangles, the two pathological hallmarks of Alzheimer’s disease, is still unclear. Increasing evidence suggests that amyloid-β peptide activates multiple regulators of cell cycle pathways, including transcription factors CDKs and E2F1, leading to hyperphosphorylation of tau protein. However, the exact pathways downstream of amyloid-β-induced cell cycle imbalance are unknown. Here, we show that PAX6, a transcription factor essential for eye and brain development which is quiescent in adults, is increased in the brains of patients with Alzheimer’s disease and in APP transgenic mice, and plays a key role between amyloid-β and tau hyperphosphorylation. Downregulation of PAX6 protects against amyloid-β peptide-induced neuronal death, suggesting that PAX6 is a key executor of the amyloid-β toxicity pathway. Mechanistically, amyloid-β upregulates E2F1, followed by the induction of PAX6 and c-Myb, while Pax6 is a direct target for both E2F1 and its downstream target c-Myb. Furthermore, PAX6 directly regulates transcription of GSK-3β, a kinase involved in tau hyperphosphorylation and neurofibrillary tangles formation, and its phosphorylation of tau at Ser356, Ser396 and Ser404. In conclusion, we show that signalling pathways that include CDK/pRB/E2F1 modulate neuronal death signals by activating downstream transcription factors c-Myb and PAX6, leading to GSK-3β activation and tau pathology, providing novel potential targets for pharmaceutical intervention.
研究领域:
神经科学
·
分子生物学
·
病理学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2016
·Vol. 27
·期 8
·8254-8270
·
IF 1.798
Xi Junhua,Ding Mingye,Dai Jianbin,Pan Yajing,Chen Daqin,Ji Zhenguo
In this paper, Yb 3+ /Er 3+ co-doped hexagonal NaYF 4 have been successfully synthesized by solvothermal, thermal decomposition, hydrothermal and molten salt method, respectively. The crystal structures, particle morphologies, sizes and corresponding upconversion luminescent properties of β-NaYF 4 :Yb 3+ /Er 3+ nano/microcrystals are systemically studied. It is interesting to observed that the temperature-dependent UC luminescent properties of β-NaYF 4 :20 %Yb 3+ , 2 %Er 3+ samples are strongly related to their synthesis methods. Comparison of the upconversion luminescence and thermal sensitivity between the β-NaYF 4 :Yb 3+ /Er 3+ samples reveals that β-NaYF 4 :Yb 3+ /Er 3+ sub-microplates synthesized by thermal decomposition method have much stronger green emission intensity, better luminescent thermal stability and higher temperature sensitivity. This findings are relevant to the application of β-NaYF 4 :Yb 3+ /Er 3+ in optical temperature sensors and to the further understanding of the UC luminescent mechanism.
研究领域:
材料科学
·
光学
✓ 已验证
DOI
高影响因子
Oxidative Medicine and Cellular Longevity
·
2022
·
IF 7.31
Chao Sun,Lei Wang,Dan-dan Du,Jian-bo Ji,Xiao-xia Yang,Bing-fang Yu,Peng-fei Shang,Xiu-Li Guo
Downregulated DSC2 involved in the metastasis of cancers. Unfortunately, its role on the development of gastric cancer (GC) and the potential mechanisms remain unclear. Bioinformatics analysis, Western blot, qRT-PCR, and immunohistochemistry were performed to detect the DSC2 levels of human GC and normal stomach tissues. The role of DSC2 and the downstream signaling in gastric carcinogenesis were explored by using GC specimens, GC cells with different DSC2 expression, inhibitors, and mouse metastasis models. We found that the level of DSC2 decreased significantly in GC tissues and cells. Recovered DSC2 inhibited the invasion and migration of GC cells both in culture and in xenografts. Mechanistically, DSC2 could not only decrease Snail level and nuclear BRD4 level by forming DSC2/BRD4, but also inhibit nuclear translocation of β-catenin. We concluded that DSC2 inhibited the metastasis of GC, and the underlying mechanisms were closely related to the regulation on nuclear translocation of BRD4 and β-catenin. Our results suggest that DSC2 may serve as a novel therapeutic target for GC.
研究领域:
肿瘤学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
BIOORGANIC CHEMISTRY
·
2025
·
IF 4.7
Menglong Zhao,Danqing Wu,Weiqian Qiu,Hongchen Zhang,Min Chen,Haixia Zhou,Xiaoxia Ye,Zhong Han,Xiangchao Shi
4-anilinoquinazoline scaffold, a key structure in the development of EGFR-TKI anticancer drugs such as Gefitinib and Erlotinib, continues to drive advancements in non-small cell lung cancer (NSCLC) therapy. In this study, we synthesized a series of novel derivatives ( B3–B11 ). Structure-activity relationship (SAR) analysis revealed that electron-donating groups para-substituted at the 4-anilino position significantly enhanced antiproliferative activity. Notably, B10 incorporating an acrylamide group at the 4-anilino position demonstrated the most potent activity against NSCLC, with an IC 50 of 1.28 μM in A549 cells, nearly six times more effective than Gefitinib (7.81 μM). Acute toxicity studies confirmed that B10 is safe at doses below 50 mg/kg. In vivo studies using an A549 xenograft model showed a 46 % tumor growth inhibition (TGI) with B10 (25 mg/kg), surpassing the 21 % inhibition of Gefitinib at the same dose. Mechanistic studies revealed that B10 inhibited the AKT/GSK-3β/β-catenin signaling pathway, downregulating Wnt target genes (c-Myc, c-Jun), and reduced COX2 expression and IL-8 levels, showcasing its dual anti-inflammatory and antitumor effects. These results position B10 as a promising NSCLC therapeutic candidate, combining potent efficacy with a favorable safety profile, and enhance our understanding of the SAR and mechanism of action of 4-anilinoquinazoline derivatives.
研究领域:
肿瘤学
·
药理学
·
分子生物学
·
药物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CATALYSIS TODAY
·
2022
·
IF 6.562
Mingsheng Luo,Chenmeng Li,Qinglong Liu,Zhi Yang,Yatao Wang,Hongjuan Li
Direct hydrogenation of CO to alcohols through Fischer-Tropsch reaction process is taken great interest in recent years. In this study, a series of β-Mo 2 C/γ-Al 2 O 3 catalyst are prepared to investigate the influence of carbon and molybdenum precursor, calcination temperature, as well as the molar ratio of the carbon precursor to molybdenum precursor compounds on catalyst microstructures and the catalytic performance. The microstructure characteristics of the catalysts were examined with XRD, BET, ICP, SEM and CO-TPD. The performance test of the catalysts for syngas-to-alcohol was investigated in a fixed-bed reactor. Properly excessive amount of hexamethylenetetramine as carbon precursor calcined at a high temperature (≥700 ℃) favors the formation of high crystalline β-Mo 2 C on γ-Al 2 O 3 carrier. Catalysts with different amount of Mo were prepared to study the effect of active metal loading on microstructure and catalytic performance. The results show that the particle size of β-Mo 2 C and CO dissociation adsorption capacity, as well as the CO conversion increases with raised Mo loading. The selectivity of all higher alcohols reached the maximum when 25 % of Mo was loaded.
研究领域:
催化
·
化学工程
·
材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2025
·
IF 9
Zhihong Dong,Ying Zhu,Xuexi Zhang,Xinliang Zhang,Xueting Zhao,Jiefeng Pan
Covalent organic frameworks (COFs) are ideal materials for engineering molecular sieves membranes. Colloidal COFs play an important role in the preparation of defect-free COF membranes. Herein, we present the alkali-mediated approach to synthesize uncharged β -ketoenamine-linked COFs with high colloidal dispersibility that can serve as preferred membrane-forming materials. The colloidal β -ketoenamine-linked COFs are synthesized in the single solution-phase system and alkali is employed to regulate the reversible dynamic amine-aldehyde condensation process by directing the weak hydrogen bond networks. The colloidal COFs obtained from alkali-mediated synthesis exhibit promoted crystallinity and outstanding dispersity for membrane construction. Furthermore, the alkali-mediated synthesis approach is universal and suitable for synthesizing a series of colloidal β -ketoenamine-linked COFs without the limitations of charged monomers or frameworks. The colloidal COFs are further assembled into COF membranes by vacuum-assisted assembly. Thanks to the intrinsic regular pore structure of COFs, the COF membranes exhibit high permeability (58 L m −2 h −1 ·bar −1 ) and can achieve accurate screening of dye molecules. This work provides a facile and scalable synthesis strategy for uncharged colloidal COFs, and can be employed to develop COF membranes for on-demand molecule separation.
✓ 已验证
产品关联
DOI
高影响因子
Arabian Journal of Chemistry
·
2015
·
IF 3.725
Shifeng Li,Yanming Shen,Min Xiao,Dongbin Liu,Lihui Fan
Controlled release formulations have been proven to have potential in overcoming the drawbacks of conventional plant growth regulators. Novel controlled-release formulation of β -naphthoxyacetic acid (BNOA) intercalated MgAl-layered double hydroxides (LDHs) was prepared by co-precipitation method. The effects of temperature, pH value, and release medium on BNOA release were studied and the releasing mechanisms were discussed. The results of release show that the increase of temperature induces the increase of BNOA release extent. The release rate and accumulated release amount of BNOA are found to be dependent to the anion in the aqueous solution in the order of CO 3 2− > SO 4 2− > Cl − . Moreover, the pH is the key controlling factor for the BNOA release processes, and with strong acid medium, the release character of BNOA is different from those at pH 7 and 12, which accompanies with fast collapse of LDHs nanolayered structure. The nanohybrid of BNOA intercalated LDHs possessed good controlled release properties and the BNOA release character from the nanohybrid fitted pseudo-second-order model in neutral medium.
研究领域:
材料科学
·
农业科学
·
植物生理学
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Shumin Zhang,Kexin Zou,Qingmin Chen,Jie Wei,Tianhui Jiao,Min Chen,Yi Xu,Quansheng Chen,Xiaomei Chen
Developing a fast, accurate, and simple detection method for food allergens is imperative to prevent food allergy. In this study, an on–off-on photoelectrochemical (PEC) aptasensor with dual-signal amplification was constructed based on a covalent organic frame (COF) film to detect β-lactoglobulin (BLG) and Ara h1. Specifically, a uniform and stable TFTA COF film was in situ grown as a PEC signifier on the surface of an indium tin oxide (ITO) electrode. BLG recognition triggered enzyme-assisted circular signal amplification to introduce non-conductive SiO 2 -modified DNA (S0) to the platform, effectively quenching the PEC signal for ultra-sensitive detection of BLG. Upon adding Ara h1, SiO 2 -DNA was released from the sensing platform to restore the PEC signal, and hemin was added to form a G-quadruplex/hemin compound for secondary signal amplification. Thus, sensitive detections of BLG and Ara h1 were achieved with low detection limits (18 ng/mL for BLG and 2.6 ng/mL for Ara h1) and wide linear ranges (0.05–5 µg/mL for BLG and 0.01–0.2 µg/mL for Ara h1). The dual-amplification and dual-target-responsive PEC aptasensor provides a new idea to develop PEC analysis methods for multi-targets in food and environmental monitoring.
研究领域:
食品科学
·
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
KEY ENGINEERING MATERIALS
·
2017
·
IF 0
Song Tao Cai,Jian Xin Luo,Hong Wei Liu
β-Cyclodextrin polymer were prepared by inverse-phase suspension polymerization using epichlorohydrin (EPI) as crosslinker. The architecture has been characterized by Fourier transform infrared spectra (FT-IR) and thermogravimetric analysis (TGA), which confirmed that cyclodextrin polymer have been synthesized successfully. β-Cyclodextrin polymer (EPI-CD) can interact with a variety of hydrophobic compounds to form inclusion complexes, such as phenol. Experimental results indicated that the adsorption reached to 58.51umol/g under the initial concentration of 120mg/L at 298K. The adsorption isotherm was fitted to the Langmuir and Freundlich models. From the experimental data, it may be inferred that EPI-CD polymer was an effective adsorbent for the removal of phenol in wastewater treatment.
研究领域:
环境科学
·
材料科学
✓ 已验证
DOI
危险品化学品经营许可证（带存储）