PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2025
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IF 4
Meilin Li,Cuilin Huang,Liya Xiong,Xiao Yang,Charles A. Powell,Ronghui Wen,Muqing Zhang
Sugarcane leaf scald, caused by Xanthomonas albilineans , is a significant bacterial disease that poses a substantial threat to global sugar production. The quorum-sensing (QS) system in X. albilineans presents a promising target for anti-virulence strategies. Here, we demonstrate that penicillic acid exhibits potent antibacterial activity against X. albilineans , with a minimum inhibitory concentration (MIC) of 6 μg/mL and an effective concentration for 50 % inhibition (EC₅₀) of 3.703 μg/mL. Transcriptome analysis, molecular docking, and surface plasmon resonance (SPR) confirmed that penicillic acid competitively binds the sensory histidine kinase RpfC, disrupting DSF-mediated QS signaling. At sub-inhibitory concentrations (½ MIC), penicillic acid significantly suppressed twitching, swimming, and swarming motility by downregulating genes associated with type IV pili, flagellar assembly, and chemotaxis. At full MIC, direct binding of penicillic acid to the FlgE and CheW proteins further impaired motility. These findings elucidate the molecular mechanism of penicillic acid and support its potential as a quorum-sensing inhibitor for biocontrol of sugarcane leaf scald.
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Applied Food Research
·
2025
·
IF 6.2
Shenchao Dai,Yilin Gao,Zhaozheng Yin,Tian Ding,Ziyi Hu,Daxi Ren
The rising consumption of Silkie chicken has underscored the challenge of its short shelf life, which results from the predominant distribution in chilled, bulk form. This study aims to screen lactic acid bacteria-derived postbiotics with antimicrobial activity and to fabricate postbiotics-loaded gelatin-chitosan composite films with enhanced antimicrobial properties via electrospinning technology for retarding the spoilage of Silkie chicken. The postbiotics derived from Lactobacillus plantarum ZJUIDS18 (GQ1501) exhibited significant antibacterial activity against five common spoilage bacteria ( Escherichia coli ATCC 25,922, Staphylococcus aureus ATCC 25,923, Pseudomonas aeruginosa ATCC 9027, Salmonella enterica serovar Typhimurium ATCC 14,028, and Listeria monocytogenes CMCC 54,007), with inhibition zone diameters of 2.00 ± 0.50 mm against P. aeruginosa and 1.17 ± 0.09 mm against Listeria . A comprehensive evaluation of the physicochemical and preservative properties of the composite films was conducted. The results demonstrated that increasing the postbiotics content led to notable improvements in film performance. Specifically, the film incorporating 15 % postbiotics showed a maximum decomposition temperature of 323.9 °C, a water contact angle of 51.8°, a tensile strength of 1.12 MPa, and an elongation at break of 3.69 %. Application of the 15 % postbiotics film on Silkie chicken during 10-day storage at 4 °C significantly reduced the total viable count by 2.59 log CFU/g and decreased the total volatile basic nitrogen (TVB-N) content by 18.70 mg/100 g compared to the control. Furthermore, it effectively delayed lipid and protein oxidation, thereby extending the shelf life of Silkie chicken by 4 days. These findings confirm the promising potential of the developed postbiotics-loaded film for application in active food packaging.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Lihao Lei,LinFeng Yang,Lan Li,Shuangli Zhang,Shuai Wu,Rongyu Li,Xianhui Yin,Yue Ma,Zhuo Chen
Postharvest losses, specifically due to Penicillium italicum disease, pose a significant challenge to the mandarin industry. To enhance mandarin preservation efficacy, this study developed a chitosan (CS)-based berberine sulfate/alpha-lipoic acid composite film (CS@BS/ALA) and evaluated its effects on mandarin preservation. Among the various concentrations, 4 % CS demonstrated optimal wettability and adhesion. Incorporating BS/ALA into CS formed a dense double-network structure that reduced oxygen permeability. CS@BS/ALA treatment effectively inhibited weight loss, delayed fruit softening, maintained gloss, and preserved the brix acid ratio. It also enhanced antioxidant capacity by reducing malondialdehyde (MDA) content and sustaining superoxide dismutase (SOD) and phenylalanine ammonia-lyase (PAL) activities. Furthermore, antimicrobial tests revealed pronounced inhibitory effects of CS@BS/ALA against Penicillium italicum . These findings indicate that CS@BS/ALA extends mandarin shelf life by regulating respiratory metabolism, enhancing antioxidant defenses, and suppressing microbial infection, thereby providing an innovative solution for reducing postharvest losses.
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CARBOHYDRATE POLYMERS
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2025
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IF 12.5
Hao Xu,Yanan Zheng,Guangling He,Xiaohui Jiang,Xuefeng Yan,Liangmin Yu
Carbon-based materials, owing to their abundance, highly tunable dielectric constants, and easily controllable microstructures, are among the most important electromagnetic wave (EMW) absorbing materials. However, they still suffer from the limitation of a narrow effective absorption bandwidth (EAB). In this work, we propose a water-induced self-assembly strategy of agar with NaCl sacrificial crystalline support to construct agar aerogel-derived porous carbon (AAPC) featuring hierarchical porosity, heteroatom doping, and conductive networks for lightweight and broadband electromagnetic wave absorption. Under conditions of low density (11.08 mg/cm3) and a low filling ratio (2 %), AAPC 4 –700 achieves an EAB of 7.68 GHz at a thickness of 2.55 mm, with a minimum reflection loss (RL min ) of −50.0 dB. In addition, AAPC exhibits excellent thermal insulation and flame-retardant properties, enabling its potential application in extreme environments. This study elucidates the EMW dissipation mechanism of AAPC and opens a new pathway for the design of lightweight EMW absorbers with broad EAB.
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International Journal of Nanomedicine
·
2025
·
IF 6.5
Size Wu,Chengfang Wang
Purpose: To investigate whether multi-focused acoustic radiation force impulse (MF-ARFI) applied to murine xenograft liver tumors prior to intravenous administration of doxorubicin-loaded PEGylated liposomes (DOX@Lip) can enhance drug delivery efficiency through modulating the enhanced permeation and retention effect of the tumor, reduce side effects, and improve antitumor effect.Materials and Methods: DOX@Lip and tumor-mimetic matrices were synthesized and characterized. Huh-7 cells and DOX@Lip were exposed to MF-ARFI and observed. MF-ARFI was applied to both tumor-mimetic matrices and saline with DOX@Lip to assess displacement effects. Subsequently, murine xenograft models were established and underwent MF-ARFI preconditioning before DOX@Lip injection. Tumor volume dynamics and body weight changes were longitudinally monitored. Terminal assessments included histopathology (H&E), apoptosis (TUNEL), and molecular profiling (BCL-2 and BAX by Western blot) of tumors and major organs.Results: There was no significant difference in the live/dead cell staining results between the Huh7 cells with and without MF-ARFI. There was no significant difference in cell apoptosis rates of Huh7 cells between DOX@Lip and DOX@Lip+MF-ARFI. MF-ARFI exposure induced measurable displacement of DOX@Lip in both tumor-mimetic matrices and saline. Mice receiving combined DOX@Lip and MF-ARFI treatment exhibited significantly attenuated tumor growth (p< 0.05) and slight weight loss, which were significantly different from DOX and DOX+MF-ARFI treatments. Cardiac histopathology revealed no significant differences in myocardial toxicity between DOX@Lip and DOX@Lip+MF-ARFI groups relative to PBS controls. Conversely, tumors from the DOX@Lip+MF-ARFI group demonstrated distinct histopathological alterations compared to other groups. TUNEL staining results indicated a relatively higher level of cell apoptosis in mice treated with DOX@Lip+MF-ARFI. Molecular analyses showed MF-ARFI pretreatment significantly reduced BCL-2 expression (p< 0.05) while elevating the BAX/BCL-2 ratio versus DOX@Lip monotherapy.Conclusion: Preconditioning xenograft tumors with MF-ARFI prior to DOX@Lip administration faciliates DOX@Lip delivery and significantly enhances antitumor effect while reducing cardiotoxicity. This combinatorial strategy demonstrates translational potential for optimizing liposomal chemotherapeutic delivery and effect.Plain Language Summary: Doxorubicin (DOX) can be used for the therapy of some liver cancers ineligible for surgery. However, DOX has severe side effects in high concentration. Using liposomes to encapsulate DOX to form nanoscale DOX@Lip can control the release of DOX in the blood circulation and reduce side effects. DOX@Lip can pass the tumor capillary and accumulate in the tumor stroma and release sustainably. However, its release efficiency can be impacted by the tumor stroma status. If the tumor stroma becomes stasis due to necrotic tumor and other cellular debris after previous treatments, the subsequently administrated drug will be difficult to pass the capillaries to accumulate in the tumor stroma. Multi-focused acoustic radiation force impulse (MF-ARFI) is a kind of low-intensity ultrasound, with it irradiation of liver tumor, the structures of tumor stroma can occur push, pull, and displacement, facilitating the drug leak out the capillaries, promoting the waste substances in the stroma moving away. Therefore, using MF-ARFI before nanoscale drug administration can facilitate the drug delivery and distribution in the tumor. The results of this study showed that using MF-ARFI irradiated xenograft liver tumor of mice followed by intravenous infusion of DOX@Lip significantly enhanced anti-cancer effect and reduce side effects.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2025
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IF 9
Tunga Kuhana Arsene,Miaomiao Tian,Yatao Zhang
The increasing demands of modern industrialization underscore the need for efficient separation and purification technologies. Multifunctional nanofiltration (NF) membranes are gaining prominence as a means to lower energy consumption, enhance efficiency, and increase process flexibility. This study explores the development of thermally stable NF membranes through interfacial polymerization (IP), employing organophosphorus bis(4-aminophenyl) phenylphosphonate (BAPP) and trimesoyl chloride (TMC) as monomers in the aqueous and organic phase, respectively. The optimized BAPP-4 membrane (containing 0.1550 w/v% BAPP), which presented a nodular buried structure, exhibited exceptional water permeability (9.23 L m −2 h −1 bar −1 ) and high Na 2 SO 4 rejection (98.52 %). Furthermore, the membrane exhibited enhanced antibacterial properties, achieving 98.00 % Escherichia coli removal, attributed to hydrogen bonding and electrostatic interactions involving amide and phosphorus groups. The BAPP-4 membrane also showed rapid flux recovery in antifouling tests, primarily due to its surface hydrophilicity. Notably, the utilization of BAPP increased the rigidity of the cross-linked polyamide network, leading to improved thermal stability. This research presents a facile and effective approach for developing high-performance and multifunctional NF membranes.
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BIOMACROMOLECULES
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2025
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IF 5.4
Shunlan Chen,Jing Liu,Dapeng Li,Ying Wen,Yiwan Huang,Shijun Long,Xuefeng Li
Bioadhesive hydrogels of favorable tribological attributes have attracted intensive attention for their potential usage due to the large benefits they can bring to surgical critical care. We report an all-natural polymer-based and bilayer-integrated asymmetrical Janus hydrogel bioadhesive patch for efficient myocardial infarction (MI) repair. We developed a low-friction polyelectrolyte composite biohydrogel (Bio-PEC hydrogel), CA, using naturally occurring cationic chitosan quaternary ammonium salt (HACC) and anionic sodium hyaluronate (HA). We further developed a high-adhesion Bio-PEC hydrogel, TA@CA, by incorporating tannic acid (TA). Ultimately, we integrated them into a bilayer asymmetrical Janus hydrogel bioadhesive, CA|TA@CA, which exhibited excellent bulk mechanical properties and favorable one-sided adequate adhesion to myocardial tissue (fracture strength 0.98 MPa, fracture strain 330%, and interfacial toughness 50.87 J/m2). Additionally, the in vivo myocardial repair surgery using the CA|TA@CA Janus hydrogel has been shown to inhibit ventricular remodeling more effectively. Such CA|TA@CA Janus hydrogel bioadhesives may have high potential as medical patches for MI repair.
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POLYMER ENGINEERING AND SCIENCE
·
2025
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IF 3.2
Ahmad Ali,Jiaqi Yu,Qasim Qasim,Faizan Ikhlaq,Yangxufei Huang,Shun Yao
This study presents the pioneering development and comprehensive characterization of argan oil (AO)-loaded polyacrylamide (PAm) hydrogel (AO-PAm), establishing its multifunctional potential for biomedical applications. Systematic evaluation revealed that the AO-PAm hydrogel combines excellent mechanical properties (90% transparency, > 25 cm stretchability) with remarkable biological activity, demonstrating: (1) potent antibacterial effects against E. coli (1.2 ± 0.2 mm inhibition zone), P. aeruginosa (0.7 ± 0.2 mm), and S. aureus (0.6 ± 0.2 mm); (2) exceptional antioxidant capacity (97.6% DPPH scavenging at 75 μg/mL); and (3) pH-responsive swelling behavior. GC–MS analysis identified key bioactive compounds (β-pinene, camphene, undecanal, β-bisabolene) in AO that contribute to these properties, while microscopic characterization confirmed successful AO encapsulation within the PAm matrix. The hydrogel exhibited controlled drug release following Higuchi kinetics ( R 2 = 0.9547), with enhanced performance at physiological pH (6.5–6.8). These findings position AO-PAm hydrogel as a promising platform for advanced wound care, transdermal drug delivery, and daily healthcare applications, combining the therapeutic benefits of AO with the structural advantages of a synthetic hydrogel matrix.
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Microorganisms
·
2025
·Vol. 13
·期 7
·1698
·
IF 4.2
Yue Cheng,Qian Fu,Junjia Xu,Xinhua Niu,Lin Liu,Jiaqi Wang,Jingwen Quan,Qingyue Yu,Baoyan Chi,Haitao Li,Rongmei Liu
Prometryn and acetochlor are herbicides used to control weeds in farmlands and other areas. They enter the soil through direct application, residual accumulation in crops, and atmospheric deposition. The pollution of their residues in the environment has attracted people’s attention. Bioremediation is one of the main methods to solve such problems. In this study, the effects of prometryn and acetochlor-degrading strain NM-1 on soil enzymes, soil microbial communities, and physiological indexes of soybean seedlings during soil remediation were studied, and the relationship between them was discussed. The results showed that 81.54% of prometryn (50 mg·L−1) and 89.47% of acetochlor (50 mg·L−1) were degraded within 15 days after NM-1 inoculation in soil. NM-1 positively affected soil enzyme activities and soil microbial communities, and the abundance of beneficial bacteria in soil increased. More importantly, the inoculation of strain NM-1 under prometryn and acetochlor stress significantly increased plant height, root length, root volume, water content, chlorophyll concentration, and root activity of soybean. The results of these studies showed that the NM-1 strain showed significant potential in bioremediation in order to provide technical support for solving the problem of prometryn and acetochlor pollution.
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ACS Nano
·
2025
·
IF 16
Xianyou Sun,Zihan Jin,Shaoxiang Zhang,Peng Lin,Xueming Zhang,Jiaying Sun,Yanchi Zhang,Yong Zhou,Yanjie Hu,Zhuoru Huang,Jiahao Hu,Shichao Tian,Guangqing Ren,Ping Wang,Liubing Kong,Hao Wan
Urine, abundant in disease-related biomarkers, offers advantages such as easy collection and noninvasiveness. Portable biochemical sensors are ideal for efficient on-site applications due to their flexibility and accessibility. However, existing sensors face challenges in complex sample operations and limited sensor renewal, hindering large-scale and convenient urine analysis. This study presents the “lab on pipette” (LOP) concept to address these issues, enabling efficient on-site urine analysis. The LOP system integrates a multifunctional pipette tip and a miniaturized system, facilitating the detection of multiple biomarkers in urine. Laser-induced graphene (LIG) technology fabricates an electrochemical electrode array on flexible polyimide, and custom-designed electrical interfaces ensure quick plug-and-play connections. Gold nano dendrites, Prussian blue (PB), and nickel hexacyanoferrate (NiHCF) form a multilayer functional interface, enabling highly sensitive detection of pH, hydrogen peroxide, glucose, and ascorbic acid with pH compensation. NiHCF doping enhances PB stability, while nanoalumina-doped porous enzyme membranes improve sensor repeatability and reliability. Gold nano dendrites anchor the enzyme membrane on curved surfaces, ensuring robust performance. The LOP achieves detection limits of 7.19 and 8.57 μM for glucose and ascorbic acid, respectively. Its low cost, disposability, portability, and reliability highlight its potential for on-site biochemical detection and personalized health monitoring.
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POLYMER TESTING
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2025
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IF 6
Guohao Li,Lingli Shang,Dapeng Li,Yiwan Huang,Shijun Long,Xuefeng Li
The antifouling capabilities of zwitterionic hydrogels have been well established, yet, drawbacks such as single antifouling performance and osmotic pressure-driven swelling in water, which lead to poor mechanical properties and low bonding strength, respectively, remain obstacles to practical applications. In this work, silane-modified polyampholyte (S-PA)-based hydrogel coatings with high adhesive strength and multi-scale antifouling ability were prepared by introducing silane onto the hydrogel surfaces. The resulting S-PA hydrogel coatings exhibited as high as 1980 J m -2 adhesion, due to the covalent bonding between the vinyl groups in PA and silanols in silane molecules. Furthermore, the S-PA hydrogel coatings demonstrated good protein adsorption resistance (the protein adsorption capacity of bovine serum protein was 5 mg g -1 ) and bacteriostatic ability, which endows the coating's multi-scale antifouling effect together with its excellent anti-diatom adhesion property (the adhesion area of navicular on S-PA hydrogel was as low as 7.43 mm 2 ). The high adhesive strength and multi-scale antifouling ability of the S-PA hydrogel coatings presented in this work may provide a new route toward marine antifouling strategies.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2025
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IF 7.7
Jinhang Li,Yue Li,Chi Hu,Lingfeng Yuan,Jiaojiao Liu,Huilin Wen,Jinhui Xie,Jing Zhang,Ziyi Yu
High-throughput sorting of microorganisms from genetic libraries is crucial for various bi- ological applications, yet existing methods often rely on exogenous markers or bulk-scale measurements that fail to capture the intricate dynamics of cellular communication. Quorum sensing, a ubiquitous intercellular communication mechanism, offers a universal signal for sorting but requires close cell proximity, and coculturing different species is constrained by interspecies competition. We address these challenges by employing core-shell microcompart- ments in microfluidics that spatially segregate microbial species while supporting controlled coculture. Through encapsulation, species such as Escherichia coli , Synechococcus elongatus , and Pseudomonas aeruginosa are immobilized in distinct core or shell compartments, creating a controlled microenvironment for intercellular communication. By integrating this system into a microfluidic workflow, we achieve unprecedented levels of precision and scalability, enabling the real-time detection and sorting of microorganisms based on their native communication pathways. This approach leverages the unique synergy between core-shell microcompartments and microfluidics, offering a versatile platform for high-throughput cell sorting with minimal genetic modification, greatly enhancing the use of quorum sensing as a natural biomarker for precise microorganism screening.
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