SENSORS AND ACTUATORS B-CHEMICAL
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2023
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IF 8.4
Lulu Song,Tiantian Ma,Fangfang Gong,Leiliang He,Yilin Wang,Qiongwen Zhang,Shuying Zhang,Yongjun Wu,Li-e Liu,Fei Yu
The abnormal expression of DNA methyltransferase1 (DNMT1) leads to change of genome methylation pattern, which may result in the occurrence and development of cancer. Therefore, the determination of DNMT activity is of great significance for cancer diagnosis and drug screening. In this study, we developed a novel method to detect DNMT1 activity using self-assembly nucleic acid probe signal amplification based on a fluorescence assay. The semi-methylated Biotin-S1'-S2' with sticky ends was fixed to the magnetic beads through the affinity of streptavidin and Biotin . Afterward, pre-prepared poly Tetramethylrhodamine (TAMRA) was added for base complementary pairing with Biotin-S1'-S2' via its sticky end when DNMT1 is present and Bss HII shearing is blocked by fully methylated bilayers, thus, the amplified fluorescent signal can be detected. The results showed that the fluorescence intensity of the system was positively correlated with the concentration of DNMT1 in the concentration range of 1–100 nmol/L, and the detection limit was as low as 0.5 nmol/L. The method is simple, highly visualized and successfully applied for the recovery of DNMT1 activity in serum samples. Thus, the method shows great potential for application in clinical diagnosis related to DNMT1.
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JOURNAL OF SOLID STATE CHEMISTRY
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2023
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IF 3.3
Jianbo Xiong,Jiali Chen,Yu Han,Jianguo Ma,Shujuan Liu,Zhenzhen Xu,Xiaoting Liu,Xiaolan Tong,Jianqiang Luo
Uranium extraction from environment is not only conducive to the sustainable development of the nuclear energy industry but also significant to the remediation of radioactive contamination. Herein, effective adsorption sites for uranium extraction were introduced via the in situ growth of cobalt vanadate porous nanospheres on the graphene oxide (GO) layer (GO@Co 2 VO 4 PNSs). Uranium adsorption capacity of the GO@Co 2 VO 4 PNSs composite promoted significantly with 221.6% promotion compare to the pristine single GO and 80.8% promotion to the pristine Co 2 VO 4 PNSs. The adsorption efficiency also enhanced distinctively to the single components of either GO or Co 2 VO 4 PNSs at a wide pH value span from 3 to 6. The reusability efficiency kept at 89.94% even after five cycles of adsorption-desorption. XPS measurements and DFT calculations reveal that the adsorption affinity mainly originated from the oxygens of the cobalt vanadate porous nanospheres . What's more, the GO@Co 2 VO 4 PNSs composite show effective uranium adsorption under a wide pH range, which paved the real application in the spent fuel disposal. The outstanding adsorption capacity, reliable reusability, and excellent removal efficiency further provide a theoretical guidance and potential application for environmental uranium-pollution remediation.
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MOLECULES
·
2023
·Vol. 28
·期 5
·2272
·
IF 4.6
Zhangxi Gao,Mei Xiang,Mingyang He,Weiyou Zhou,Jiayao Chen,Jiamin Lu,Zeying Wu,Yaqiong Su
Catalytic conversion of CO2with the surplus glycerol (GL) produced from biodiesel manufacturing has attracted much academic and industrial attention, which proves the urgent requirement for developing high-performance catalysts to afford significant environmental benefits. Herein, titanosilicate ETS-10 zeolite-based catalysts with active metal species introduced by impregnation were employed for coupling CO2with GL to efficiently synthesize glycerol carbonate (GC). The catalytic GL conversion at 170 °C miraculously reached 35.0% and a 12.7% yield of GC was obtained on Co/ETS-10 with CH3CN as a dehydrating agent. For comparison, Zn/ETS- Cu/ETS-10, Ni/ETS-10, Zr/ETS-10, Ce/ETS-10, and Fe/ETS-10 were also prepared, which showed inferior coordination between GL conversion and GC selectivity. Comprehensive analysis revealed that the presence of moderate basic sites for CO2adsorption-activation played a crucial role in regulating catalytic activity. Moreover, the appropriate interaction between cobalt species and ETS-10 zeolite was also of great significance for improving the glycerol activation capacity. A plausible mechanism was proposed for the synthesis of GC from GL and CO2in the presence of CH3CN solvent over Co/ETS-10 catalyst. Moreover, the recyclability of Co/ETS-10 was also measured and it proved to be recycled at least eight times with less than 3% decline in GL conversion and GC yield after a simple regeneration process through calcination at 450 °C for 5 h in air.Keywords:CO2;glycerol;glycerol carbonate;ETS-10 zeolite;cobalt-based catalyst
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PubMed
高影响因子
FOOD HYDROCOLLOIDS
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2023
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IF 10.7
Juncheng Zhu,Yang Cheng,Zhiying Ouyang,Yuxin Yang,Liang Ma,Hongxia Wang,Yuhao Zhang
Dry-spraying transglutaminase (TG) was explored as surface modification agent for enhancing the surimi structures and promoting its practical 3D printing application. Glycerol functionalization, 3D extruding critical parameters, TG based surface crosslinking, practical cooking loss, and innovative applications of 3D-printed surimi were investigated. Rheological performances (shear ramp, strain sweep and thixotropy tests) indicate that glycerol could enhance excellent pseudo plasticity (fluid behavior index n < 1), high mechanical strength & strain resistance (high G′, and wide linear viscoelastic region 2.51%), and outstanding thixotropy (high recovery rates, up to 61.47%) of surimi, owing to that glycerol could promote the hydration of surimi myosin and prevent protein aggregation by stabilizing aggregation-prone intermediates. The further optimization of the extruding parameters (nozzle diameter 1.00 mm and “Rectilinear” filling structure) would improve the surimi dimensional printing deviations, and increase surimi gel strength (up to 22.29 g mm) by regulating interlayer bonding zones. Compared with chemical crosslinking agents NaCl and CaCl2 for modifying surimi, surface crosslinking based on dry-spraying TG would better raise the printable height efficiency (97.50%), improve the surimi texture, and enhance suitable water distribution, resulting in fine and dense network by hydrophobic interactions, disulfide bonds and non-disulfide covalent bonds. Subsequently, the cooking loss tests and International Dysphagia Diet Standardization Initiative tests proved that 3D-printed surimi could be classified as a potential transitional food for dysphagia groups. Surface crosslinking based on dry-spraying TG suggests a bright promise in practical high-accuracy, sophisticated 3D printing of surimi and holds potential in dysphagia diets and innovating food products.
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ENERGY & FUELS
·
2023
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IF 5.3
Jingyuan Ma,Shaocong Pang,Yuxiu An
Drilling fluids with high suspending capacity without high viscosity are required in drilling operations, such as horizontal wells and highly deviated wells, with the “cutting bed”. Non-viscosifying rheological modifiers that can enhance the rheological properties of polymer drilling fluids are urgently needed. This study reports the application of a choline-chloride-based deep eutectic solvent (Gly-DES) as a rheological modifier for polymeric water-based drilling fluids. The rheological effects of Gly-DES on bentonite/polymer fluids were evaluated by high-temperature rheological testing, rheological model fitting, and rheological parameter (yield point, fluidity index, gel strength, etc.) analysis. The results showed that 2–4 wt % Gly-DES can improve the rheological properties of polymer drilling fluid in the temperature range of 150 °C. The addition of Gly-DES significantly improved the rheological parameters of drilling fluids, such as the yield point, gel strength, low shear rate viscosity, etc. At the same time, the viscosity of drilling fluids was always stable at different temperatures, which means that Gly-DES can help improve the suspension and cutting carrying capacity of drilling fluids without significantly increasing the viscosity of drilling fluids. Mechanistic analysis revealed that Gly-DES was connected to the polymer and bentonite through hydrogen bonds and ionic bonds, which enhanced the cross-linked network structure of the polymer at high temperatures and promoted the formation of weak gels in Na bentonite suspensions. Gly-DES can simultaneously enhance rheology, inhibit clay hydration, and improve filter cake lubricity and has the potential to be a multifunctional drilling fluid treatment agent.
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Petroleum Science
·
2023
·
IF 5.6
Zhong-Zheng Xu,Ming-Wei Zhao,Yi-Ning Wu,Jia-Wei Liu,Ning Sun,Zi-Zhao Wang,Yi-Ming Zhang,Lin Li,Cai-Li Dai
The membrane method based on adaptive wettability shows great advantages in oil-water separation. At present, researches focus on the excellent application performance of the membrane material, while the quantitative analysis of interactions in oil-water separation is rarely recognized. Herein, we constructed an adaptable wettability membrane with multiple polymer networks by polydopamine (PDA) and mussel-inspired amphiphilic polymer. Based on the Owens three-probe liquid method, the surface energy of the modified membrane was verified to meet the adaptive wettability conditions, with surface energies ( γ S ) of 147.6 mJ m −2 (superhydrophilic/underwater superoleophobic) and 49.87 mJ m −2 (superhydrophobic/superoleophobic), respectively. The adhesion or repulsion of the membrane to the oil phase under different conditions during the separation process was quantified by the chemical probe AFM technique. In addition, the oil-water selective separation mechanism was further analyzed in a simplified membrane microchannel model. The results show that the different wetting produces capillary additional pressure in opposite directions, resulting in different energies to be overcome when the oil or water passes through the microchannels, thus achieving selective separation.
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高影响因子
MOLECULES
·
2023
·Vol. 28
·期 8
·3384
·
IF 4.6
Bing Han,Peifeng Chen,Jiaxuan Guo,Hongliang Yu,Shaojing Zhong,Dongmei Li,Chunhong Liu,Zhibiao Feng,Bin Jiang
The development of intelligent indicator film that can detect changes in food quality is a new trend in the food packaging field. The WPNFs-PU-ACN/Gly film was prepared based on whey protein isolate nanofibers (WPNFs). Anthocyanin (ACN) and glycerol (Gly) were used as the color indicator and the plasticizer, respectively, while pullulan (PU) was added to enhance mechanical properties of WPNFs-PU-ACN/Gly edible film. In the study, the addition of ACN improved the hydrophobicity and oxidation resistance of the indicator film; with an increase in pH, the color of the indicator film shifted from dark pink to grey, and its surface was uniform and smooth. Therefore, the WPNFs-PU-ACN/Gly edible film would be suitable for sensing the pH of salmon, which changes with deterioration, as the color change of ACN was completely consistent with fish pH. Furthermore, the color change after being exposed to grey was evaluated in conjunction with hardness, chewiness, and resilience of salmon as an indication. This shows that intelligent indicator film made of WPNFs, PU, ACN, and Gly could contribute to the development of safe food.Keywords:whey protein isolate nanofibers;anthocyanin;edible film;food packaging;food spoilage
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PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2023
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IF 7.7
Jiguang Huang,Haiping Chen,Run Qi,Jihao Yang,Zhaohao Li,Heng Zhang
Coal fly ash (CFA) is a substitute material for ceramic membrane which can effectively reduce the preparation cost. However, CFA based ceramic membranes usually show insufficient mechanical strength for application. Revealing pore-forming mechanisms of different pore-forming agents in membranes preparation is of great significance to improve mechanical strength without harming permeability. In this work, three different pore-forming agents, carbon powder, dolomite and dextrin, were adopted to prepare CFA membranes. Characters including porosity, pore size distribution and permeability of membranes were comparatively analyzed. Effects of pore-forming agents on membranes were discussed. The membrane with dextrin at 1200 °C sintering showed the best comprehensive properties. Its porosity, flexural strength and Darcian permeability coefficient were 39.46 %, 35.56 MPa and 3.84 × 10 −14 m 2 , respectively. Application in oil-in-water emulsion separation indicated higher than 94 % oil rejection. This research provides experimental bases for improving performances of low-cost CFA membranes, thereby conducive to engineering applications.
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COMPOSITES COMMUNICATIONS
·
2023
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IF 8
Ling Wang,Hao Wang,Wei Xiao,Qiancheng Wan,Jiefeng Gao
Wearable piezoresistive sensors possessing outstanding flexibility and breathability have aroused growing interests of researchers due to their promising applications in wearable electronics, health monitoring, etc. The development of waterproof and oil-proof piezoresistive sensors that respond to both tensile and compressive strains is still a great challenge. Herein, a two-step dip-coating method, i.e., ion reduction assisted phase separation and subsequent fluorination, is used to prepare amphiphobic rubber foam composites (ARFC). The ARFC shows excellent photothermal and electro-thermal conversion performance. The ARFCs with high electrical conductivity can be served as both strain and pressure sensors, demonstrating a dual sensing mode. The ARFC exhibits a relatively low detection limit (1%), wide working range (0–150%) and a gauge factor of 34.2 under 100%–150% strain. Also, the strain/pressure sensor exhibits outstanding cyclic sensing performance and can realize real-time monitoring of different body motions. Its amphiphobicity and dual sensing mode further broaden the application in smart wearable electronics.
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JOURNAL OF MEMBRANE SCIENCE
·
2023
·
IF 9.5
Yanrui Wang,Hailin Chang,Shu Jiang,Jialong Chen,Jinlong Wang,Heng Liang,Guibai Li,Xiaobin Tang
The interfacial polymerization (IP) plays a fundamental role in the fabrication of polyamide (PA) nanofiltration (NF) membranes with high ion selectivity and water permeability, which has enormous value for the increasing permeate quality and production in desalination. Herein, this study adopted a weak-polar organic solvent of ethyl formate (EF) to effectively regulate the IP process between piperazine (PIP) and trimesoyl chloride (TMC). The engineered PA separate layer conferred a uniform and ultrafine pore size (∼0.195 nm), exhibited a precise solute separation, and maintained a desirable permeability (18.4 ± 0.9 L m −2 h −1 bar −1 ). Molecular dynamics (MD) simulation indicated that EF involvement would significantly reduce the interfacial tension to motivate a fast and abundant diffusion of amine monomers from the aqueous phase into organic phase during the IP reaction, contributing to engineering a PA film with higher crosslinking degree. The optimized PA NF membranes conferred a more efficient rejection for divalent anions and cations (e.g., 99.5% for Na 2 SO 4 and 98.8% for MgCl 2 ). Remarkably, the membrane endowed a fairly high mono/divalent ion selectivity at sub-angstrom size, such as 145 for Cl − /SO 4 2− and 61 for Na + /Mg 2+ . The outstanding NF performance successfully broke the "trade-off" bound of perm-selectivity and was superior to the most "state-of-the-art" NF membranes. Overall, the co-solvent based IP strategy was beneficial to developing high-performance NF membranes without any significant changes to the existing fabrication process, and its facile and cost-effective merits were full of significance to the membrane manufacture.
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APPLIED CLAY SCIENCE
·
2023
·
IF 5.6
Qiming She,Minyang Qiu,Kejin Li,Jiahui Liu,Chunhui Zhou
Montmorillonite (Mt) as a solid acid catalyst or support has been widely used in catalytic reactions . However, the existence and catalytic activities of its surface basic sites have rarely been revealed. Here, the surface and structure of Na-Mt are modulated by thermal treatment, providing both acidic and basic sites for the transesterification of glycerol with dimethyl carbonate (DMC) to glycerol carbonate (GLC). The experimental results showed that the thermally activated Na-Mt exhibited bifunctional catalytic properties in glycerol transesterification. The Na-Mt calcined at 400 °C had a basic site density of 1.38 mmol/g and led to a glycerol conversion of 96.8% and a GLC yield of 94.5%. Edge surfaces of Na-Mt provided M II (M III ) atoms as Lewis acidic sites for facilitating the generation of glyceroxide anions from activated glycerol and -M-OH groups as Brønsted and Lewis basic sites for enhancing the carbonyl activation of DMC. This work revealed the co-existence of acidic and basic sites over thermally activated Na-Mt for synergetic catalysis in the transesterification of glycerol to GLC, making the development of Mt-based materials as bifunctional catalysts for one-pot acid-base catalytic processes possible.
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INDUSTRIAL CROPS AND PRODUCTS
·
2023
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IF 5.9
Xin Li,Jing Wei,Lezhen Lin,Guodong Zheng
Akebia trifoliata (Thunb.) Koidz is gaining popularity, because of its high nutritional, economic and medicinal values. The purpose of this study was to optimize the extraction conditions of A. trifoliata (Thunb.) Koidz polysaccharide (ATKP) and investigate moisturizing activity to prepare a novel moisturizing cosmetic material. It has been observed that using polysaccharide yield as the evaluation criterion showed optimal extraction at a material-liquid ratio of 1:20, an ultrasonic temperature of 80 °C, an ultrasonic time 50 min and ultrasonic power of 175 W. Furthermore, three deproteinization methods of ATKP process, including Sevag, enzymatic hydrolysis and their combination method, were evaluated. The deproteinization efficiency of the Sevag and enzymatic hydrolysis combination method was the highest, with a deproteinization efficiency of 87.52 %. The moisturizing capacity of ATKP was significantly higher than that of chitosan and sodium alginate . ATKP-containing skin cream, particularly the combination of 4.2 % glycerol and 0.8 % ATKP, had an excellent moisturizing effect, and was added to cosmetic formulations to form skin creams. The findings revealed that ATKP has the potential synergistic effect with glycerol in the development of cosmetic formulations, which could be used as a new material for natural moisturizers.
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