TALANTA
·
2021
·
IF 6.057
Huazheng Yu,Yanhong Li,Aisheng Huang
Sialic acid (SA) is a crucial component of glycoproteins and glycolipids on the cellular membrane, which is essential for maintaining the function of cell membranes, such as cell recognition and communication. Simultaneously, sialic acid plays a significant role in many physiological and pathological processes. Hence, it is urgent to develop a simple and sensitive strategy for determining sialic acid. In this work, a new metal-organic framework called UiO-66-NH 2 @B(OH) 2 has been designed and synthesized for the recognition and detection of sialic acid. The boronic acid functional group in UiO-66-NH 2 @B(OH) 2 can bind to a diol moiety of the glycerol side chain of sialic acid, which will attenuate or even quench the fluorescence of UiO-66-NH 2 @B(OH) 2 , thus opening a new road to detect sialic acid. Based on the measurement results, sialic acid can be quantitatively measured in a linear range of 0.05–2.5 mmoL/L with the UiO-66-NH 2 @B(OH) 2 probe. The detection limit of sialic acid is as low as 0.025 mmol/L. Furthermore, the boronic-acid functionalized probe UiO-66-NH 2 @B(OH) 2 displays high sensitivity and high selectivity to recognize the sialic acid in mouse serum samples. Therefore, the developed UiO-66-NH 2 @B(OH) 2 can be used as a promising probe to identify and detect sialic acid in the practical application.
研究领域:
分析化学
·
生物化学
·
材料科学
✓ 已验证
DOI
高影响因子
Journal of Nanoelectronics and Optoelectronics
·
2021
·Vol. 16
·期 11
·1834-1843
·
IF 0.961
Yuan Wenfeng,Yang Jia,Yuan Mengmeng,Fan Jiacheng,Li Ke,Wang Libo,Zhou Aiguo
In this paper, a novel conductive hydrogel was made by a one-pot method at 60 °C for 12 hours in one step without any cross-linking agent. The hydrogel was composited of Ti3C2 MXene nano slices, poly(vinyl alcohol) (PVA) and poly-N-(2-hydroxyethyl acrylamide)
(PHEAA), named as MXene/PVA/PHEAA hydrogel. Compared with previous methods to make MXene hydrogel, this one-pot method was facile and fast. The as-prepared hydrogel was highly stretchable with the breaking elongation of 1300% and tensile strength of 1.1 MPa. MXene nano sheets dispersed in
the hydrogel formed a three-dimensional conductive network, which increased the strength and conductivity of the gels. The conductivity changed with the gel’s deformation and could be applied as strain sensors. The strain sensors made of the hydrogels were highly stretchable, bendable
and flexible with the response range of 1%–300% and a large gauge factor of 4.23. The sensors can be applied to detect human movements such as finger bending, leg bending, water drinking. The MXene/PVA/PHEAA hydrogel made by this facile one-pot method is promising to make strain sensors
for health detection.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
DOI
高影响因子
Landslides
·
2019
·Vol. 16
·期 7
·1313-1324
·
IF 4.252
Yang Wentao,Wang Yunqi,Sun Shao,Wang Yujie,Ma Chao
Detecting slope movement before landslides occur in mountain regions is crucial for disaster reduction. In October 2018, a gigantic landslide occurred on the Jinsha River, causing dammed-lake breach flood 500 km downstream. In this work, we used 25 Sentinel-2 images from November 2015 to August 2018 to explore the capability of this high temporal resolution optical images in detecting slope movement before the Jinsha River landslide. Normalized difference vegetation index (NDVI) was calculated to composite temporal profiles using all Sentinel-2 images. With this NDVI time series, unsupervised K-means classifier was applied to initially classify the study area and find the best thresholds for automatically extracting landslide scars in the image series. These extracted landslide scars were validated using interpreted results from two high spatial resolution images of similar dates in 2015 (user’s accuracy 89.7%, producer’s accuracy 83.6%) and 2018 (user’s accuracy 90.8%, producer’s accuracy 74.9%). After validation, extracted landslide scars of different years were counted and displayed in an RGB composite image to highlight slope movement. In addition, monotonous decrease/increase of NDVI was also observed, indicating continuous expansion of landslide scarps and movement of landslide head along the slope on the landslide surface. This work demonstrated the capability of Sentinel-2 time series images to capture slope movement with short revisit time at low cost. By incorporating other environmental information (such as elevation), this proposed method has the potential to consistently map pre-landslide slope movements over a large region.
研究领域:
遥感地质学
·
环境灾害监测
·
地理信息系统
·
山地灾害防治
·
地球观测
✓ 已验证
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2021
·
IF 8.128
Lixia Wang,Rongfei Ye,Panming Jian,Jiangyong Liu
Metal-free catalysts with environmental friendless, cost-competitiveness and less susceptibility to leaching and poisoning over metal-based catalysts, have revolutionized in the catalysis domain. In this respect, we herein report the first application of cheap and abundant pumpkin-derived N -doped porous carbon for the reduction of 2-methyl-4-nitrophenol assisted by NaBH 4 . The obtained catalyst is cost-competitive, efficient and robust, with an attractive mass-normalized rate constant of 4.73 s −1 g −1 and good recycling performance. Systematical analyses demonstrate that the 2-methyl-4-nitrophenol reduction reaction catalyzed by the N -doped carbon proceeds through the Langmuir–Hinshelwood kinetics and the performance enhancement benefits from the strong adsorption and activation of the substrates induced by the electronic modulation in the carbon framework via N -doping. This study opens up new avenues for the high-value use of pumpkin as well as the development of metal-free strategy in more catalytic applications.
研究领域:
催化化学
·
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Jiao Chen,Hongye Qin,Yaping Pan,Jinli Wu,Jianan Liu,Wanying Lin,Jun Jiang,Enzuo Liu,Junwei Sha,Fang He,Liying Ma,Naiqin Zhao
It is necessary and challenging to develop platinum-based electrocatalysts with low Pt content and high performance for industrial development in a suitable strategy. Herein, ultralow Pt (0.35 wt%) loading Ni(OH) 2 nanosheets (named as 2-Pt/Ni(OH) 2 ) with cataphracted structure is synthesized on nickel foam by one-step hydrothermal process. It only needs an overpotential of 34 mV to achieve a current density of 10 mA cm -2 without any significant decrease for 40 hours. This excellent performance of hydrogen evolution reaction (HER) is attributed to the fact that the cataphracted structure not only has a larger active area and more active sites, but also effectively limits the size of Pt nanoparticles to 2~3 nm, which results in a higher active quality. In addition, the relatively simple and easily operational one-step method can reduce the Pt content to 0.35 wt% significantly. This facile strategy opens up new approaches in the rational design and synthesis of low loading Pt electrocatalysts with eminent electrocatalytic activity for industrial production.
研究领域:
材料科学
·
电化学
✓ 已验证
DOI
高影响因子
TETRAHEDRON
·
2024
·
IF 2.1
Nan Wang,Yuanyuan Huang,Mengke Wang,Weichun Huang,You Zi
Herein, a new approach for the synthesis of ( Z )- N -alkenyl-2-pyridones from 2-pyridones and propiolates using phosphine catalysis under mild conditions is reported. This dual Z -diastereoselective and N -selective reaction demonstrates excellent selectivity, yielding sole ( Z )- N -alkenyl-2-pyridones. Furthermore, scale-up studies confirm its practical applicability, with consistent efficiencies observed.
研究领域:
有机化学
·
合成化学
✓ 已验证
DOI
高影响因子
iScience
·
2023
·
IF 5.8
Yao Li,Zi-Yu Liang,Hai-Lin Wang
Summary Red blood cells supply the oxygen required for all human cells and are in demand for emerging blood-loss therapy. Here we identified N6-methyl-2′-deoxyadenosine (6mdA) as an agonist that promotes the hyperproliferation of burst-forming unit erythroid (BFU-E) progenitor cells. In addition, 6mdA represses the apoptosis of erythroid progenitor cells (EPCs). Combined use of with SCF and EPO enabled cultures of isolated BFU-E to be expanded up to 5,000-fold. Transcriptome analysis showed that 6mdA upregulates the expression of the EPC-associated factors c-Kit, Myb, and Gata2 and downregulates that of the erythroid maturation-related transcription factors Gata1, Spi1, and Klf1. Mechanistic studies suggested that 6mdA enhances and prolongs the activation of erythropoiesis-associated master gene c-Kit and its downstream signaling, leading to expansion and accumulation of EPCs. Collectively, we demonstrate that 6mdA can efficiently stimulate the EPC hyperproliferation and provide a new regenerative medicine recipe to improve ex vivo generation of red blood cells.
研究领域:
血液学
·
分子生物学
·
再生医学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
ChemMedChem
·
2017
·Vol. 12
·期 4
·319-326
·
IF 3.225
Tingjunhong Ni,Ran Li,Fei Xie,Jing Zhao,Xin Huang,Maomao An,Chengxu Zang,Zhan Cai,Dazhi Zhang,Yuanying Jiang
Graphical Infections unanchored ! A series of novel 2-aminonicotinamide derivatives were designed, synthesized, and evaluated for in vitro antifungal activity. Compounds 11 g and 11 h have excellent activity against Candida albicans , with MIC 80 values of 0.0313 μg mL −1 , and exhibit broad-spectrum antifungal activity against fluconazole-resistant C. albicans , C. parapsilosis , C. glabrata , and Cryptococcus neoformans , with a MIC 80 range of 0.0313–2.0 μg mL −1 . Further investigation suggested that compound 11 g targets the cell wall and decreases glycosylphosphatidylinositol anchor content on the cell surface of C. albicans . Based on the structures of the reported compounds G884 [ N -(3-(pentan-2-yloxy)phenyl)nicotinamide], E1210 [3-(3-(4-((pyridin-2-yloxy)methyl)benzyl)isoxazol-5-yl)pyridin-2-amine], and 10 b [2-amino- N -((5-(3-fluorophenoxy)thiophen-2-yl)methyl)nicotinamide], which inhibit the biosynthesis of glycosylphosphatidylinositol (GPI)-anchored proteins in fungi, a series of novel 2-aminonicotinamide derivatives were designed, synthesized, and evaluated for in vitro antifungal activity. Most of these compounds were found to exhibit potent in vitro antifungal activity against Candida albicans , with MIC 80 values ranging from 0.0313 to 4.0 μg mL −1 . In particular, compounds 11 g [2-amino- N -((5-(((2-fluorophenyl)amino)methyl)thiophen-2-yl)methyl)nicotinamide] and 11 h [2-amino- N -((5-(((3-fluorophenyl)amino)methyl)thiophen-2-yl)methyl)nicotinamide] displayed excellent activity against C. albicans , with MIC 80 values of 0.0313 μg mL −1 , and exhibited broad-spectrum antifungal activity against fluconazole-resistant C. albicans , C. parapsilosis , C. glabrata , and Cryptococcus neoformans , with a MIC 80 range of 0.0313–2.0 μg mL −1 . Further studies by electron microscopy and laser confocal microscopy indicated that compound 11 g targets the cell wall and decreases GPI anchor content on the cell surface of C. albicans .
研究领域:
药理学
·
微生物学
·
化学
✓ 已验证
DOI
PubMed
高影响因子
FITOTERAPIA
·
2019
·
IF 2.431
Yue Yang,Haihui Xie,Yueming Jiang,Xiaoyi Wei
Averrhoa carambola L. (Oxalidaceae) was widely cultivated for fruits (star fruit), whereas the value of leaves remains to be discovered. Our study on the leaves yielded five flavan-3-ols ( 1 – 5 ) and two 2-diglycosyloxybenzoates. Their structures were determined by spectroscopic and chemical methods. Epicatechin-(5,6-bc)-4β-( p -hydroxyphenyl)-dihydro-2(3 H )-pyranone ( 1 ) and benzyl 2-β- d -apiofuranosyl-(1 → 6)-β- d -glucopyranosyloxybenzoate ( 6 ) were new structures. 6-( S −2 -Pyrrolidinone-5-yl)epicatechin ( 4 ) and 6-( R -2-pyrrolidinone-5-yl)epicatechin ( 5 ) were obtained as monomeric diastereomer for the first time and their absolute configurations were determined by electronic circular dichroism (ECD) computation. Epicatechin-(7,8-bc)-4α-( p -hydroxyphenyl)-dihydro-2(3 H )-pyranone ( 2 ), epicatechin-(7,8-bc)-4β-( p -hydroxyphenyl)-dihydro-2(3 H )-pyranone ( 3 ), and methyl 2-β- d -apiofuranosyl-(1 → 6)-β- d -glucopyranosyloxybenzoate ( 7 ) were not previously reported from the genus Averrhoa . Compounds 1 – 5 showed more potent 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical cation and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities and ferric reducing antioxidant power (FRAP) than l -ascorbic acid. Meanwhile 1 and 3 exhibited lipase and α-glucosidase inhibitory activities, respectively. The results clarified the structures of flavan-3-ols and 2-diglycosyloxybenzoates in the leaves and their antioxidant, lipase, and α-glucosidase inhibitory activities.
研究领域:
植物化学
·
药理学
·
食品科学
✓ 已验证
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CATALYSIS
·
2014
·
IF 1.552
Zhongkui Zhao,Renzhi Li,Yu Li
A clean and highly efficient synthesis of 1-amino-2-acetylanthraquinone via reductive isoxazole ring cleavage of 3-methylanthra[1,2- c ]isoxazole-6,11-dione catalyzed by trace copper using hydrazine hydrate as a clean reducing agent and water as a green reaction medium under mild reaction conditions was investigated. Various transition-metal catalysts were screened for the reductive ring-opening reaction, and Cu(NO 3 ) 2 was shown to be an excellent catalyst. A conversion of 97.2% and 1-amino-2-acetylanthraquinone selectivity greater than 95% were obtained in the presence of 2.6 mol% Cu(NO 3 ) 2 (turnover number 38) with 1.3 equiv. of hydrazine hydrate for 2 h in water. The structure of the product was confirmed by 1 H nuclear magnetic resonance spectroscopy and mass spectrometry ; the main byproduct was hydroxyl-substituted 1-amino-2-acetylanthraquinone. A possible reaction mechanism for the copper-catalyzed ring cleavage of 3-methylanthra[1,2- c ]isoxazole-6,11-dione with hydrazine hydrate was proposed.
研究领域:
有机化学
·
环境化学
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Bokai Ma,Fujian Xu,Mian He,Yuqing Lin,Guanghui Hu,Mei Zhang,Xinying Zhao,Weili Liu
Rhodamine B hydrazide (RBH) was prepared as a new derivatizing reagent for residual formaldehyde in a drug such as N-butyl-2-cyanoacrylate (NBCA). RBH reacted with formaldehyde rapidly at room temperature, and the resulting derivative can be detected sensitively at 238 nm. After optimizing the derivatization and chromatographic separation conditions, RBH as a pre-column derivatizing reagent has been successfully used for the analysis of formaldehyde in real NBCA samples by high-performance liquid chromatography (HPLC). The detection limit of the method is 0.004 μg/mL for formaldehyde, and the recovery is 94.0%-96.6%. Most notably, compared with the previous HPLC methods, the developed method has the advantages of mild derivatization temperature, short derivatization time and high sensitivity.
研究领域:
分析化学
·
药物分析
✓ 已验证
DOI
高影响因子
AAPS PHARMSCITECH
·
2012
·Vol. 13
·期 3
·836-845
·
IF 1.432
Han Lina,Zhao Yuefang,Yin Lifang,Li Ruiming,Liang Yang,Huang Huan,Pan Shirong,Wu Chuanbin,Feng Min
In the present study, we developed novel insulin-loaded hyaluronic acid (HA) nanoparticles for insulin delivery. The insulin-loaded HA nanoparticles were prepared by reverse-emulsion-freeze-drying method. This method led to a homogenous population of small HA nanoparticles with average size of 182.2 nm and achieved high insulin entrapment efficiencies (approximately 95%). The pH-sensitive HA nanoparticles as an oral delivery carrier showed advantages in protecting insulin against the strongly acidic environment of the stomach, and not destroying the junction integrity of epithelial cells which promise long-term safety for chronic insulin treatment. The results of transport experiments suggested that insulin-loaded HA nanoparticles were transported across Caco-2 cell monolayers mainly via transcellular pathway and their apparent permeability coefficient from apical to basolateral had more than twofold increase compared with insulin solution. The efflux ratio of P
app (B to A) to P
app (A to B) less than 1 demonstrated that HA nanoparticle-mediated transport of insulin across Caco-2 cell monolayers underwent active transport. The results of permeability through the rat small intestine confirmed that HA nanoparticles significantly enhanced insulin transport through the duodenum and ileum. Diabetic rats treated with oral insulin-loaded HA nanoparticles also showed stronger hypoglycemic effects than insulin solution. Therefore, these HA nanoparticles could be a promising candidate for oral insulin delivery.
研究领域:
药剂学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）