CRYSTENGCOMM
·
2018
·Vol. 20
·期 26
·3657-3665
·
IF 3.304
G. Q. Wang,L. Y. Li,Y. N. Feng,H. Yu,X. H. Zheng
In this study, a novel dual-mode luminescence phosphor KBaLu(MoO4)3:Tb3+,Yb3+ has been synthesized by high-temperature solid-state reactions, and the crystal structures have been determined for the first time. Single-crystal X-ray diffraction data reveal that its space groups are C2/c. The crystalline structure is constituted by K/BaO8 distorted square antiprisms, distorted LuO8 polyhedra and two kinds of distorted MoO4 tetrahedra, which form chains lying along the c-axis. Spectroscopic properties and energy transfer mechanisms are investigated by detailed analyses of emission and excitation spectra and excited-state lifetimes. The KBaLu(MoO4)3:Tb3+,Yb3+ phosphor shows dominant green emissions in the visible region, which are due to 5D4 → 7F5 transition of Tb3+ under excitation light in ultraviolet region as well as a 980 nm laser, and the CIE coordinates are close to the Society of Motion Picture and Television Engineers (SMPTE) standard values for primary green (0.310, 0.595). With the increasing temperature, the luminescence of KBaLu(MoO4)3:Tb3+,Yb3+ phosphor decreases almost linearly with the CIE coordinate variation from (0.326, 0.616) to (0.327, 0.530), which results in photoluminescence color variation from primary green to yellowish-green. The present results demonstrate the successful synthesis of a dual-mode luminescence material KBaLu(MoO4)3:Tb3+,Yb3+ phosphor with both up- and down-conversion luminescence properties; moreover, it can be used as a potential spectrum converter in display lighting and anti-counterfeiting.
研究领域:
光学材料
·
固体化学
·
发光材料
·
晶体学
·
光谱学
✓ 已验证
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2016
·
IF 2.74
Xue Wang,Mingjie Wang,Zhiyun Chen,Xiaoyi Tao,Weiguo Shen
The liquid-liquid equilibrium data and the specific heat capacities for the binary room temperature ionic solution {1-butanol + 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide} have been reported. The corresponding critical exponents β and α were obtained and found to be well coincided with the 3D-Ising criticality. The critical complete scaling theory was applied to successfully describe the asymmetric behavior of the diameter of the coexistence curve with the consideration of the heat capacity related term. The large values of the reduced critical density, the reduced critical temperature and the critical amplitude related to the heat capacity were deduced, which indicated a solvophobic criticality of the studied system.
研究领域:
物理化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Jinkui Wang,Tong Bu,Qianqian Cui,Zizhe Li,Qing Xu,Zhixian Jiang,Yexuan Mao,Xianqing Huang,Lianjun Song,Dapeng Peng,Xiya Zhang
Simultaneous detection of carbofuran (CBF) and 3-hydroxy carbofuran (3-OH-CBF) in fruits and vegetables is important due to their high toxicity and widespread use in pest control. However, most lateral flow immunoassay (LFA) approaches only detection CBF. To overcome this limitation, two haptens, 6-((2,2-dimethyl-2,3-dihydrobenzofuran-7-yl)oxy)hexanoic acid and 6-((((2,2-dimethyl-2,3-dihydroben zofuran-7-yl)oxy)carbonyl)amino)hexanoic acid, named H1 and H2, were designed for broad-spectrum antibody detection of CBF and 3-OH-CBF. The highly specific monoclonal antibody (mAb) 3F4 based on hapten H1 only recognized CBF, while broad-spectrum mAbs 1D3, 6B7 and 6E8 based on hapten H2 recognized both compounds. The carbon bond to the hydroxyl group of 3-OH-CBF adopts a 28.55° angle relative to the same carbon bond of CBF, explaining the specificity of the H1-based mAb against CBF. After optimizing the antigen-antibody combination, a sensitive colloidal gold-LFA (CG-LFA) strip was constructed based on mAb 1D3 and H2-BSA for CBF and 3-OH-CBF detection following simple sample pretreatment. The established LFA had a limit of detection of 0.49–6.63 ng/mL, and adequate recoveries (69.3–105.7 %) for CBF and 3-OH-CBF in 26 fruit and vegetable samples.
研究领域:
环境科学
·
食品安全
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2024
·
IF 2.7
Jiajie Xue,Lingjun Kong,Wei Lin,Jiasheng Zhang,Youzhi Zhu
The development of sensitive breast cancer biomarker (CA 15-3) measurement techniques is of great significance for the early diagnosis and therapeutic monitoring of breast cancer worldwide. Herein, we have designed a split-type photoelectrochemical (PEC) immunoassay platform for the ultrasensitive determination of CA 15-3 using In2O3/ZnIn2S4 heterojunctions with high photovoltaic conversion performance as a photoactive substrate. Efficient photocurrent-responsive In2O3/ZnIn2S4 was formed by surface loading narrow-bandgap ZnIn2S4 on MOF-derived wide bandgap In2O3 nanomaterials. Subsequently, a sandwich immunoreaction was performed using an alkaline phosphatase (ALP)-labeled secondary antibody as a signaling indicator to produce large amounts of ascorbic acid by enzymatic biocatalysis. Since ascorbic acid is an excellent electron donor capable of promoting photogenerated electron separation in semiconductor materials, it enhances the anodic photocurrent of In2O3/ZnIn2S4-modified electrodes. Under the optimal catalyst loading and immunoreaction time, the In2O3/ZnIn2S4-based PEC immunoassay showed a good photocurrent response to CA 15-3 over a wide detection range from 1.0 mU mL−1 to 10 000 mU mL−1 with a limit of detection of 0.45 mU mL−1. Owing to the excellent photocurrent response of In2O3/ZnIn2S4 to ascorbic acid and the split-type immunoassay protocol, the constructed PEC immunosensing platform has the advantages of high precision, anti-interference, and acceptable accuracy, and the results are equivalent to those of commercial enzyme-linked immunosorbent assay (ELISA) kits.
研究领域:
生物医学工程
·
肿瘤学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2024
·
IF 8.1
Xiang Liu,Ailing Zhang,Hao Yu,Liang Chen,Lei Zhang,Yong Zhao,Jialu Li,Weiqi Zhang,Zhiting Zhou,Yongyan Zhou,Yuanyuan Wang,Jian Zhen Ou
Alkaline water electrolysis is one of the primary drivers of hydrogen energy development, and anion exchange membranes (AEMs) play a dual role in ensuring both conductivity and safety. However, traditional polymer AEMs have a wide pore size distribution and poor chemical stability, making it difficult to achieve a long-term balance between conductivity and safety of the water electrolysis system. Here, we select inorganic two-dimensional multilayer graphene oxide (GO) membranes as AEMs, using carboxylated wrinkled graphene (WG) and ethylenediamine (EDA) to create a cation-modified porous EDA-WG/GO (E-W/G) composite membrane with a 3 nm pore size. The enlarged channel size and enhanced hydrophilicity improve OH − permeability compared to the pristine GO membrane, while the strengthened hydration layer acts as a barrier to hydrophobic gases for O 2 /H 2 separation. The results show that the prepared E-W/G membrane exhibits superior current density (600 mA cm −2 ) and gas impermeability (gas purity 99.99%) compared to the commercial Fumasep FAA-3-50 membrane (590 mA cm −2 and 99.81%, respectively). Furthermore, after continuous testing for 168 h in high-temperature and alkaline environments, the E-W/G membrane maintained conductivity comparable to its initial state and showed enhanced gas impermeability. Our strategy provides new insights into the design of high-performance AEMs and is expected to contribute to the advancement of the hydrogen energy industry.
研究领域:
材料科学
·
能源技术
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2021
·Vol. 13
·期 14
·1757-1765
·
IF 2.896
Can Zhang,Xiaoxiao Wu,Dongyang Li,Jinnuo Hu,Debin Wan,Zhen Zhang,Bruce D. Hammock
As a major metabolite of pyrethroid pesticides, 3-phenoxybenzoic acid (3-PBA) can be an indicator of health risk and human exposure assessment. Based on nanobodies (Nbs), we have developed a rapid flow-through dot enzyme linked immunosorbent assay (dot ELISA) and gold nanoparticle (GNP) lateral-flow immunoassay for detecting 3-PBA. The limit of detection (LOD) values for detecting 3-PBA by flow-through dot ELISA and GNP lateral-flow immunoassay were 0.01 ng mL−1 and 0.1 ng mL−1, respectively. The samples (urine and lake water) with and without 3-PBA were detected by both nanobody-based flow-through dot ELISA and GNP lateral-flow immunoassay, as well as liquid chromatography-mass spectrometry (LC-MS) for validation. There was good consistency between the results of the immunoassays. This demonstrated that the two developed nanobody-based immunoassays are suitable for rapid detection of 3-PBA.
研究领域:
环境科学
·
分析化学
·
免疫学
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
LETTERS IN APPLIED MICROBIOLOGY
·
2014
·Vol. 59
·期 3
·306-312
·
IF 1.749
Meng F.,Ma L.,Ji S.,Yang W.,Cao B.
Bioconversion of biomass, particularly crop wastes, into biofuels is being developed as an alternative approach in meeting the high energy demand. In this study, a thermophilic bacterial strain BY‐3 that exhibits cellulolytic potential was isolated from faecal samples of Tibetan pigs; this strain was identified as Bacillus subtilis. The strain can produce cellulase when grown on various substrates, including carboxymethyl cellulose, rice straw, corn stover, soluble starch and wheat bran. The maximum cellulase activity of the strain was up to 4·323 ± 0·065 U ml−1 when cultivated in the medium containing corn stover (30 g l−1) for 24 h. The results demonstrated that corn stover is the most suitable substrate for cellulase production by the strain BY‐3. The crude cellulase of strain BY‐3 was most active at pH 5·5 and 60°C, and the enzyme in acetate buffer (50 mmol l−1) demonstrated a good stability at 60°C for at least 1 h. The crude cellulase exhibited a strong antibacterial activity against Staphylococcus aureus. The strain can be used in cost‐efficient cellulase production for bioconversion of agricultural residual biomass into biofuels.Significance and Impact of the StudyThe increased consumption of fossil fuels has caused serious energy crisis and environmental problem. Thus, an alternative energy source is necessary. Bioconversion of biomass, particularly agricultural residuals, into value‐added bioproducts, such as biofuels and chemical solvents, has received considerable attention. In this study, the newly isolated thermophilic Bacillus subtilis strain BY‐3 produces cellulase efficiently with the use of untreated corn stover as a sole carbon source. This strain possesses the thermostable cellulase that is active with diverse crop wastes with a broad pH range and is a highly promising candidate for agricultural waste management.
研究领域:
微生物学
·
生物技术
·
可再生能源
✓ 已验证
DOI
高影响因子
JOURNAL OF SURFACTANTS AND DETERGENTS
·
2010
·Vol. 14
·期 3
·317-324
·
IF 0.795
Cui Zheng-gang,Song Hong-xing,Yu Jun-jie,Jiang Jian-zhong,Wang Feng
Alkali-surfactant-polymer (ASP) flooding has been considered to be one of the useful tertiary oil recovery techniques. However, field tests in China have revealed that serious side effects may occur due to using alkali. Thus alkali-free SP flooding is more favorable in China. Unfortunately, surfactants effective in ASP flooding are usually ineffective in the absence of alkali and new surfactants need to be designed. In this paper N -(3-Oxapropanoxyl)dodecanamide as a pure compound and a mixture of homologues with narrow EO number distribution, synthesized by the reaction of lauroyl chloride with diglycolamine and addition of one ethylene oxide to coconut monoethanolamide respectively, are examined for their adaptability in SP flooding. It is found that, when mixed with betaines, both products can reduce Daqing crude oil/connate water interfacial tension to a magnitude of 10 −3 –10 −4 mNm −1 at 45 °C in a wide surfactant concentration range, 0.01–0.5 wt%, and oil displacement tests using natural cores indicates that a tertiary oil recovery of 18.6 ± 0.4% OOIP can be achieved by SP flooding with the N -(3-Oxapropanoxyl)dodecanamide as the main surfactant without adding any alkaline agent and neutral electrolyte. N -(3-Oxapropanoxyl)dodecanamide, as a nonionic surfactant without a cloud point and producible industrially from renewable materials, is an ideal surfactant for SP flooding in the absence of an alkaline agent.
研究领域:
石油工程
·
化学工程
✓ 已验证
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2014
·
IF 6.451
Zihong Sun,Zhigang Luo,Cuifen Gan,Shidong Fei,Yingju Liu,Hongtao Lei
A sensitive electrochemical immunosensor for 3-bromobiphenyl (3-BBP) detection was constructed by employing a new polydopamine coated prussian blue-mesoporous carbon (PDOP/PB/CMK-3) nanocomposite as the substrate platform and multi-horseradish peroxidase-double helix carbon nanotubes-secondary antibody (multi-HRP-DHCNTs-Ab 2 ) as the signal label. PB/CMK-3 was firstly successfully in-situ synthesized with the aid of the CMK-3 reduction, which was characterized by transmission electron microscope (TEM), infrared spectroscopy (IR), X-ray diffraction (XRD) and N 2 adsorption–desorption analysis. By using PDOP/PB/CMK-3 as the substrate, it can effectively enhance the specific surface for antigen loading due to the three-dimensional structure of the nanocomposites, while large amount of PB that fixed inside or outside the pore of CMK-3 successfully improved the electrochemical response and the PDOP film can provide a biocompatible environment to maintain the activity of antigen availability. Under the optimized conditions, the proposed immunosensor shows a good current response to 3-BBP in a linear range from 5 pM to 2 nM with a detection limit of 2.25 pM. In addition, the specificity, reproducibility and stability of the immunosensor were also proved to be acceptable, indicating its potential application in environmental monitoring.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Min Fang,Zhaojie Wang,Qing Fu,Qing Yang,Lin Xu,Yuepeng Lu,Yong Yang,Xiaoming Jiang,Yongning Wu,Zhiyong Gong,Xin Liu
The aim of this work was to develop a fluorescence method based on the polydopamine-polyethyleneimine (PDA-PEI) copolymerization, which was subsequently applied for the determination of 3-monochloropropane-1,2-diol (3-MCPD) in food contact papers (FCMs). PEI could provide an alkaline environment and then react with dopamine (DA) to produce copolymers by Michael addition and Schiff-base reactions. This copolymer has a strong fluorescence emission at 527 nm. We found that amino groups of DA and PEI could also react with 3-MCPD in an alkaline medium, which improved the morphology and fluorescence intensity of PDA-PEI copolymers. The fluorescence intensity of the polymers was linear but inversely proportional to the concentration of 3-MCPD in the range of 10.0–500.0 μg kg −1 and the detection limit was 2 μg kg −1 . The standard addition method was used in FCMs to demonstrate the practical applicability and the spiked recoveries ranged from 99.8 to 110.3 %. Finally, the levels of 3-MCPD in different FCMs (n = 70) were determined by the proposed method. The detection frequencies ranged from 25 % to 100 % and both the highest detection frequency and levels were observed in kitchen papers. More than half of the samples did not comply with the limits recommended by the German Federal Institute for Risk Assessment, suggesting that 3-MCPD released from FCMs is a major route of human exposure.
研究领域:
食品安全
·
分析化学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2015
·
IF 4.074
Cong Chen,Yan Meng,Sheng Li,Wenhua Wu,Chuanjun Liu,Xiaojuan Xu,Lina Zhang
A polysaccharide coded as PR-CA was extracted from Polyporus rhinoceros and determined to be a β-(1→3)- d -glucan with multiple branches. The weight-average molecular weights ( M w ) of PR-CA in dimethylsulfoxide (DMSO) and in water were determined with static light scattering (SLS) to be 3.57 × 10 5 and 1.79 × 10 7 , indicating existence of the single chains in DMSO and co-existence of single chains and aggregates in water. Moreover, the stiffness of single chains of PR-CA in water was directly visualized by atomic force microscopy (AFM) and transmission electron microscopy (TEM). The hollow structure of PR-CA nanofibers with width of 30–40 nm and length of ∼350 nm formed in the water/DMSO (9:1, v:v) was demonstrated by a fluorescent probe tetraphenylethylene (TPE) via aggregation-induced emission (AIE). The formation of PR-CA nanofibers was ascribed to the parallel aggregation of the extended PR-CA chains due to the hydrogen bonding and hydrophobic interaction. This work offered valuable results for promising applications of natural branched β-glucans in the biological fields of drug inclusion, delivery and disease diagnosis.
研究领域:
生物化学
·
材料科学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Biomedical Nanotechnology
·
2021
·Vol. 17
·期 4
·677-690
·
IF 4.099
Zhou Shuping,Ma Yongfang,Liu Xueke,Yu Pan,Huang Ning,Song Li,Xu Ruyue,Huo Zhen,Zhu Tao,Tang Xiaolong
The miR let-7b-5p (a kind of microRNAs) has many pathophysiological regulation effects, including human hepatocellular carcinoma (HCC) pathogenesis. This study investigated whether nanoparticle-mediated miR let-7b-5p could jointly enhance the therapeutic effect of sorafenib on HCC by
inhibiting the proliferation of HCC cells, inducing apoptosis, and reversing drug resistance. We evaluated the level of miR let-7b-5p in sorafenib-resistant HepG2 cells (HepG2R) and HepG2 HCC cells by qRT-PCR and analyzed the biological effects of hepatocellular carcinoma treated
with sorafenib with miR let-7b-5p, and further studied the toxicity of nanoparticles (Ab-miR-NPs) that deliver miR let-7b-5p mimics and target GPC3 on the surface of hepatocellular carcinoma cells. Results showed that, in HepG2 cells, the expression level of miR let-7b-5p was significantly
higher than that in HepG2R cells. Targeted nanoparticle Ab-miR-NPs mediated the delivery of miR let-7b-5p to the HCC cytoplasm and released miRNA after being broken down, down-regulating the expression of IGF1R and inhibiting AKT/mTOR and Ras/Raf signal transmission. Ab-miR-NPs
not only enhanced the proliferation of sorafenib in cultured HepG2R cells and induced cell apoptosis efficiency, but they also improved the anti-tumor activity in the mouse models. These results indicated that GPC3 antibody-modified PLGA-PLL (polylactic acid-glycolic acetic copolymer
grafted hyper-branched polylysine) loaded miR let-7b-5p polymer nanoparticles combined with sorafenib may be a new treatment strategy for HCC resistant to sorafenib.
研究领域:
肿瘤学
·
分子生物学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）