ANALYST
·
2020
·Vol. 146
·期 4
·1295-1302
·
IF 3.978
Defen Feng,Fucun Wei,Yeyu Wu,Xuecai Tan,Fang Li,Yongkuai Lu,Gaochao Fan,Heyou Han
An ultrasensitive electrochemiluminescence (ECL) biosensor was developed based on MIL-53(Al)@CdS QDs and SiO2@AuNPs for trichlorfon detection. Metal–organic frameworks (MOFs) were used as a loading platform that provided a large surface area to load targets and modified materials onto the electrode. At the same time, SiO2@AuNPs loaded plenty of AuNPs which effectively increased the ECL resonance energy transfer between the CdS QDs, so that the ECL signal was strongly quenched and resulted in an amplified response. In the range of 10−11–10−4 M, the ECL response showed a linear relationship with the concentration (logarithm) of trichlorfon, and the detection limit was 5.1 × 10−12 M (S/N = 3). When the biosensor was applied to detect trichlorfon in lettuce, broccoli, cucumber, and chives, the recoveries obtained from the spiked samples were 97%–105%, 102%–104%, 100%–104%, and 98%–104%, respectively. Thus, this novel ECL biosensor has potential applications for the analysis of trichlorfon in food samples.
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Analytical Methods
·
2019
·Vol. 11
·期 10
·1353-1360
·
IF 2.378
Xiaoyun Lin,Yanfang Wang,Miaomiao Zou,Yongnian Ni
Terbutaline sulfate (TBS) is a β2-adrenoceptor agonist, overdoses of which can cause serious harm and even be life-threatening to humans. Herein, we demonstrate a simple and facile method for the preparation of a molybdenum disulfide–gold nanoparticle composite (MoS2/AuNPs). The obtained MoS2 and MoS2/AuNPs were characterized and investigated by X-ray powder diffraction, scanning electron microscopy, and transmission electron microscopy. An MoS2/AuNPs-modified glassy carbon electrode (MoS2/AuNPs/GCE) was constructed using the dropping method and used to study the electrochemical behavior of TBS. The results indicated that the sensor had excellent electrocatalytic activity for TBS, with detection sensitivity for TBS more than 100 times higher compared with the bare GCE. With the successive addition of TBS, the peak current of the differential pulse stripping voltammogram (DPSV) of TBS was proportional to its concentration within a certain range, with a linear range of 1–85 nmol L−1 and limit of detection (LOD) of up to 0.47 nmol L−1. Furthermore, the modified electrode showed good stability, reproducibility, and anti-interference ability, meaning that it could be used to detect TBS in real samples.
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ADVANCED POWDER TECHNOLOGY
·
2021
·
IF 4.833
Xinyue Chen,Dan Liu,Sanhu Gou,Jiahui Ji,Zhiyuan Xue,Shilan Feng
A colorimetric assay based on the oriented TA-AuNPs-PEG-FITC for rapid and sensitive PABA detection was proposed. Initially, the negatively charged TA modified on the surface of AuNPs enabled rapid binding to PABA due to the electrostatic interaction and hydrogen bond, resulting in a visible color change. However, particles were aggregated in a non-oriented way, leading to an unstable testing system which failed to realize the accurate quantification. Thus, the asymmetrically functionalized TA-AuNPs-PEG-FITC were prepared, in which the stabilizer agent of HS-PEG-FITC was attached to the specific sites on the surface of TA-AuNPs. Such a way of modification resulted in an oriented aggregation manner, the addition of PABA induced the formation of oligomers. Moreover, the introduction of FITC group acted as fluorescent marker, providing a simple, fast and quantitative method for characterization of PEG chain. The molar ratio between TA-AuNPs and modified PEG-FITC was further determined. A linear regression was established in a wide range from 10 μM to 1 mM with the LOD of 6.9 μM. Finally, the sensor was successfully applied in the health food. This was the first example in which TA-AuNPs-PEG-FITC were fabricated for colorimetric detection of PABA, and an indirect method based on fluorescence marker was well used to quantify the content of PEG chain.
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JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2019
·Vol. 166
·期 10
·B821
·
IF 3.12
Pengfei Li,Zhenping Liu,Zhibin Yan,Xin Wang,Eser Metin Akinoglu,Mingliang Jin,Guofu Zhou,Lingling Shui
Insufficient or excessive consumption of Vitamin B2 (Vit B2) and Vitamin B6 (Vit B6) can cause harmful effects on human health conditions, it is therefore very important to control their content in supplements and food products. In this work, we report on a simple, sensitive and selective electrochemical sensor based on gold nanoparticles (AuNPs) on glassy carbon electrode (GCE) modified by a hybrid of polydopamine and reduced graphene (PDA-RGO), for determination of Vit B2 and Vit B6. The hybrid nanomaterial comprising PDA-RGO supported AuNPs is constructed through a one-step GCE modification process for electrochemical quantification of Vit B2 and Vit B6 in pharmaceutical formulations. Cyclic voltammetry result reveals that the effective response to Vit B2 and Vit B6 is according to the high electrical conductivity of AuNPs and the large surface area of PDA-RGO. Meanwhile, the sensor modified with a composite film shows a synergistic effect for catalyzing the analytes. Under optimized experimental conditions, these sensors show wide linear ranges of 0.02−60.0 μM and 0.03−600.0 μM, with the detection limits (LODs) of 0.0096 μM and 0.025 μM for Vit B2 and Vit B6, respectively. Additionally, the constructed sensors have been applied for quantifying Vit B2 and Vit B6 in pharmaceutical formulations. Such an effective electrochemical platform could be potentially applied for analyzing various components for therapeutic drug monitoring.
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Nanoscale
·
2024
·
IF 5.8
Jiayi Zhu,Chao Zhao,Binyun Xia,Ning Wang,Xi Chen,Xinyue Jing,Minxuan Chen,Xinrui Xu
Abnormal human immunoglobulin G (IgG) may induce the risk of immune system disorder, infectious diseases, tumors and so on. However, the current detection methods exhibit low sensitivity, which limits their practical application. In this work, an SPR optical fiber sensor (SPR-OFS) with high sensitivity is designed for label-free detection of human IgG. It is fabricated using a heterostructure optical fiber coated with Au film/AuNPs and the Ti3C2Tx MXene biofunctionalized with goat anti-human IgG by polydopamine (PDA). In the experiment, the optimal thickness of the Ti3C2Tx MXene was explored and determined to be about 93 nm by comprehensively considering the refractive index (RI) sensitivity and spectral bandwidth of the SPR sensor. When the largest figure of merit (FOM) is calculated to be 17.8279 RIU−1, its RI sensitivity was ultimately found to be 2804.5 nm per RIU. The SPR-OFS was employed to detect human IgG within the concentration range of 0–30 μg mL−1 and its sensitivity is demonstrated to be 1.7046 nm (μg mL−1)−1. The SPR-OFS was also proved to have excellent linearity, specificity and stability. The proposed sensor offers outstanding performance with simple fabrication, providing a cutting-edge bioanalytical platform with potential applications in clinical diagnosis.
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EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
·
2025
·
IF 4.7
Xin Li,Qing Wang,Chun-Rong Qin,Ai-Lin Liu,Yong Chen
Background: Cluster of differentiation 123 (CD123) prominently overexpress in various hematological malignancies and plays a crucial role in diagnosis and prognosis of leukemia. Clinical studies have demonstrated that cell-free CD123 levels also significantly influence leukemia immunotherapy outcomes. The development of novel electrochemical immunosensors addresses the need for point-of-care detection tools, thereby facilitating advancements in clinical monitoring technologies. Methods: This study presented a practical electrochemical immunosensor constructed using sandwich strategy for CD123 detection based on the modification of gold nanoparticles and poly(2-aminoterephthalic acid). Common proteins and tumor-related biomarkers found in human were selected as interference factors to evaluate the detection specificity of the electrochemical immunosensor. Further, the electrochemical immunosensor was utilized to directly detect CD123 in bone marrow supernatant from leukemia patients. Results: The constructed electrochemical immunosensor exhibited good linearity for CD123 detection over a range of 0.02 to 2.5 µg/mL, with a detection limit of 12.8 ng/mL, alongside satisfactory specificity and repeatability. Furthermore, the immunosensor was successfully employed to detect CD123 levels in the bone marrow supernatant of leukemia patients, demonstrating results that were highly consistent with those obtained via ELISA. Conclusions: The developed approach is anticipated to provide robust technical support for the long-term monitoring of leukemia patients during their diagnosis and treatment.
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ChemistrySelect
·
2026
·Vol. 11
·期 8
·e07116
·
IF 2.3
Xiongtao Yu,Haiping Xu,Rui Wang,Feng Zhou,Chunmin Yang
In this manuscript, Au nanoparticles (AuNPs) and a nanospherical conjugated microporous polymer (CMP) were prepared and then modified on the surface of screen-printed carbon electrode (SPCE) to construct an electrochemical sensor for high sensitivity and rapid detection of 3-amino-2-oxazolidinone (AOZ). The preparation process of the materials and electrodes were characterized by scanning electron microscope (SEM), transmission electron microscope (TEM), cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance (EIS), and the test conditions were optimized. Under optimal conditions, the oxidation peak current of AOZ and the concentrations show a wider linear relationship in the ranges of 1.0×10 −8 ∼1.0×10 −6 and 1.0×10 −6 ∼ 1.0×10 −4 mol/L ( R 1 2 = 0.996, R 2 2 = 0.998), respectively, and a lower detection limit which is as low as 3.3×10 −9 mol/L (S/N = 3) compared to existing methods. The reason for this result may be that CMP exhibited abundant porous structures and numerous active sites, which could significantly improve response sensitivity; AuNPs also possess high specific surface area and make the diffusion and transport of electrolyte ions easier. CMP-AuNPs nanocomposites greatly improved the electron transport rate, signal intensity and electroactive surface of SPCE, thereby improving the sensitivity of the sensor. It is worth noting that the sensor also has strong anti-interference ability, stability, and reproducibility. When applied to detect AOZ in real samples, it also showed good recovery rate. Compared with HPLC-MS/MS, the sensor also obtained good accuracy and consistency, which has great application potential for rapid on-site analysis and detection in animal-derived food not only for AOZ but also for Furazolidone (FZD).
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SURFACE REVIEW AND LETTERS
·
2024
·
IF 1.2
ZHE-NAN LIU,XIAO-YING LIU,XIAO-YUE FAN,JIN-PENG XUE,GUO-NA LI,XING LI
In this study, a novel colorimetric method is developed for the detection of arsenic and mercury ions, utilizing the surface plasmon resonance (SPR) properties of AuNPs ( AuNPs = gold nanoparticles) functionalized with APT ( APT = 5 -(3-aminophenyl) tetrazole). Theoretical calculations confirm the findings from UV–Visible spectroscopy and transmission electron microscopy analyses, indicating that APT is electrostatically bound to the surfaces of the AuNPs. The introduction of As 3 + and Hg 2 + ions triggers a coordination reaction with APT, resulting in a reduced interparticle distance among the AuNPs. This change leads to a visible color shift of the solution from wine red to bluish-gray. The detection limits for As 3 + and Hg 2 + are determined to be 0.6 and 0.24 μ g⋅ mL − 1 , respectively, distinguishable to the naked eye. A robust linear correlation is observed between the absorbance ratios and the ion concentrations, with correlation coefficients ( R 2 ) of 0.990 for As 3 + and 0.998 for Hg 2 + . These findings demonstrate that AuNPs functionalized with APT are effective for the quantitative analysis of arsenic and mercury ions. The sensor exhibits high selectivity and excellent resistance to interference, indicating its potential applicability for monitoring As 3 + and Hg 2 + in tap water and cosmetic products.
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Chunyan Ren,Guoliang Ding,Mengru Chen,Xiaojun Chen,Gang Peng
Carbon material derived from lotus petiole exhibits enzyme-like activity. • The multiple enzyme-like activities of Cu@C-AuNPs can be switched by pH. • A colorimetric-electrochemical dual-mode aptasensor was successfully constructed. • The sensor provides a detection limit as low as 4.35 × 10 −6 ng mL −1 . • The developed method is suitable for controlling AFB1 in rice. Rapid and reliable detection of aflatoxin B1 (AFB1) is of paramount significance for food safety control and public health protection. Herein, a novel lotus petiole-derived pH-switchable Cu@C-AuNPs nanozyme featuring versatile enzyme-like activities was rationally engineered to fabricate a dual-mode colorimetric-electrochemical aptasensor for the ultrasensitive and selective detection of AFB1. Benefiting from its sharp pH-switchable catalytic behavior, the as-prepared nanozyme displays prominent peroxidase-like activity under acidic conditions (pH 4.0), efficiently catalyzing H 2 O 2 -mediated oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to enable visual colorimetric readout; in contrast, it switches to distinct catalase-like activity under neutral conditions (pH 7.4), decomposing H 2 O 2 to generate oxygen and thereby producing quantifiable electrochemical signals. Under optimized experimental parameters, the colorimetric mode exhibits a linear response from 1 × 10 −2 to 1 × 10 3 ng mL −1 with a limit of detection (LOD) of 6.96 × 10 −3 ng mL −1 , whereas the electrochemical mode achieves a broader linear range of 1 × 10 −5 to 1 × 10 3 ng mL −1 with a superior LOD of 4.35 × 10 −6 ng mL −1 . This dual-signal sensing strategy not only furnishes a robust and accurate platform for AFB1 detection but also holds promising application prospects in on-site and laboratory-grade food safety monitoring. A lotus petiole-derived Cu@C-AuNPs nanozyme with pH-switchable activities enables dual-mode detection of aflatoxin B1. At pH 4.0 (colorimetric), peroxidase-like activity oxidizes TMB to oxTMB, giving an absorbance increase (LOD 6.96 × 10 −3 ng mL −1 ). At pH 7.4 (electrochemical), catalase-like activity decomposes H 2 O 2 to O 2 , producing a current decrease (LOD 4.35 × 10 −6 ng mL −1 ). This aptasensor provides reliable cross-validation for food safety monitoring. Download: Download high-res image (177KB) Download: Download full-size image
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Tao Liu,Dan Li,Wenying Hu,Amirah F. Kamaruddin,Zhisong Lu,Wei Sun
Reliable electrochemical determination of adenine (Ade) in biological samples remains challenging due to interference from structurally similar nucleobases, nonspecific adsorption, and instability of conventional sensing interfaces, which compromise selectivity and signal reproducibility. Here, we report an interface engineered electrochemical molecularly imprinted sensor based on disposable screen-printed electrodes (SPEs). The sensing platform integrates a poly( o -phenylenediamine) molecularly imprinted recognition layer with Au nanoparticles/MXene quantum dots/nitrogen-doped graphene (AuNPs/MQDs/NGR) nanocomposite transduction interface. MXene quantum dots (MQDs) provide abundant edge and terminal sites that promote uniform AuNPs nucleation and anchoring on NGR, forming a conductive and structurally stable sensing layer with reduced charge-transfer resistance. This engineered interface enables stable electropolymerization of the imprinted film and improves signal stability and electrode-to-electrode reproducibility. The sensor exhibited a linear response for Ade from 0.25 to 80.0 μM (R 2 = 0.992) with a detection limit of 0.094 μM and recoveries of 96.40–103.84% in human serum sample. This strategy provides a reproducible and selective electrochemical platform that addresses key limitations of current adenine sensors for analysis in complex biological matrices.
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MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 8
·542
·
IF 5.4
Chen Chong,Liu Yufei,Zhang Kai,Fan Qiao,Luo Tingting,Peng Shigui,Wei Lanlan,Yin Yucheng,He Min,Yu Jie
Wearable sweat sensors offer a promising technical approach for real-time personal health management due to their non-invasive, in situ, and continuous monitoring capabilities. This study presents a novel wearable non-enzymatic electrochemical sensor based on a PEDTM/rGO/AuNPs composite for continuous and non-invasive monitoring of sweat glucose. Leveraging the synergistic effects of hydroxymethyl-functionalized poly(3,4-ethylenedioxythiophene) (PEDTM), reduced graphene oxide (rGO), and gold nanoparticles (AuNPs), the sensor significantly improves the electrocatalytic oxidation efficiency of glucose. At a low working potential of 0.2 V, the sensor demonstrates high sensitivity (362 µA·mM − 1 ·cm − 2 ) and a low detection limit (6.2 µM). Density functional theory (DFT) calculations reveal the synergistic catalytic mechanism of the composite at the oxidation pathway level. To achieve stable and efficient sweat collection and real‑time analysis, the sensor is integrated with a microfluidic chip based on SDS/NaOH/PI nanofibers and a portable point-of-care testing (POCT) system, enabling on‑site visual monitoring of sweat glucose. Real‑time monitoring results indicate a strong correlation between the sweat glucose levels measured by the sensor and blood glucose concentrations. This work provides an effective material and device design strategy for constructing high‑performance, integrated non‑invasive glucose monitoring systems, offering significant theoretical value and application potential in the field of wearable health monitoring. Graphical abstract
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ACS Applied Materials & Interfaces
·
2026
·
IF 7.8
Yang Hongqun,Li Longhao,Niu Mu,Wang Hong,Zhang Yingwei
The development of highly sensitive and reliable biosensors is essential for the early diagnosis and therapeutic monitoring of cancer. Herein, we report a dual-mode fluorescence–colorimetric biosensing platform for the ultrasensitive detection of miRNA-21, integrating AuNP-supported dual-stage rolling circle amplification (RCA) with an RCA-generated multipedal binding-and-cleavage mechanism. The sensing hierarchy is initiated by target-dependent activation of the primary RCA on the gold nanoparticle (AuNP) surface, producing long DNA strands with multiple repeated recognition domains that function as numerous walker “legs” and form a DNA-rich nanogel matrix. Through these repeated domains, the RCA-generated multipedal walker repeatedly and multivalently binds fluorophore-labeled hairpin substrates on the AuNP surface, thereby facilitating APE1-mediated substrate cleavage and fluorescence recovery. The APE1-generated cleavage fragments subsequently initiate the secondary RCA following the addition of the T2 template. The two successive RCA reactions ultimately produce an Au@RCA DNA nanogel containing abundant G-quadruplex/hemin DNAzymes that catalyze a visually detectable color change. By integrating the multipedal walker-mediated multivalent binding-and-cleavage process with dual-stage RCA, the platform enables dual-mode fluorescence–colorimetric detection with efficient signal amplification and low background, achieving limits of detection of 9.854 and 790 fM for the fluorescence and colorimetric modes, respectively. Furthermore, the two complementary outputs provide independent signal readouts for cross-validation. The biosensor demonstrated excellent specificity and distinguished the endogenous responses of MCF-7, HeLa, and HEK293T cell lysates. These results support the potential of the platform for ultrasensitive tumor-associated biomarker detection, while the cell-lysate experiments represent a preliminary proof-of-concept evaluation in complex biological matrices.
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