ANALYTICAL LETTERS
·
2022
·
IF 2.267
Wentang Zhao,Mei Wu,Lisha Mei,Hongling Li
An ultra-sensitive electrochemical immunosensor for detecting carcinoembryonic antigen was prepared. Graphene oxide-gold was used as the substrate modifier of the sandwich immunosensor. The high conductivity of graphene oxide effectively enhanced the electron transfer rate. The large specific surface area of graphene and the biocompatibility of gold nanoparticles increased the number of primary antibodies. The bovine serum albumin coated silver – platinum composite was used as a secondary antibody tag to prepare signal probes. The excellent biocompatibility of bovine serum albumin increased the number of secondary antibody connections. Silver nanoparticles increased the speed of electron transfer, and platinum further enhanced electrochemical sensing capabilities by catalyzing hydrogen peroxide. The immunosensor showed good performance for detecting carcinoembryonic antigen. Under the optimal conditions, the dynamic response range for carcinoembryonic antigen was from 0.005 ng·mL−1 to 100 ng·mL−1 with a detection limit of 0.76 pg·mL−1. The immunosensor also shown high selectivity, suitable stability, and good reproducibility, which provides new capabilities for clinical carcinoembryonic antigen detection.
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高影响因子
POLYHEDRON
·
2025
·
IF 2.6
Xinying Yuan,Xue Zhang,Qionghua Zhang,Gang Zhao
Three forms of cobalt, including cobalt chloride (CoCl 2 ), cobalt (II) acetylacetonate (Co(acac) 2 ) and cobalt (III) acetylacetonate (Co(acac) 3 ), were evaluated for their interactions with bovine serum albumin (BSA). Results reveal distinct binding mechanisms among cobalt compounds. CoCl 2 bound to negatively charged residues on the BSA surface via electrostatic interactions, exhibiting weak binding affinity ( K b = (0.09–2.3) × 10 3 L·mol −1 ) and high temperature sensitivity (Δ G ° decreased significantly with increasing temperature). Co(acac) 2 partially penetrated the hydrophobic microenvironment of BSA through its hydrophobic ligands, achieving moderate binding strength ( K b = (3.24–6.84) × 10 3 L·mol −1 ) with synergistic contributions from hydrogen bonding and van der Waals forces (Δ H ° = −48.80 ± 0.01 kJ·mol −1 , Δ S ° = −89.87 ± 0.01 J·mol −1 ·K −1 ). Co(acac) 3 demonstrated the strongest binding capacity ( K b = (9.27–32.3) × 10 4 L·mol −1 ), attributed to multimodal interactions (hydrophobic embedding, coordination bonds, and hydrogen bonding) facilitated by its three hydrophobic ligands. The binding affinity of cobalt compounds to BSA increases with ligand complexity and hydrophobicity (CoCl 2 → Co(acac) 2 → Co(acac) 3 ). UV–Vis, three-dimensional fluorescence, and circular dichroism spectra further revealed that organic acetylacetonate ligands enhanced BSA structure perturbation, intensifying with ligand number. Co(acac) 3 induced significant conformational changes in BSA (19.98 % reduction in α -helix content), increased microenvironmental polarity (5 nm fluorescence redshift), and optimized spectral overlap between donor and acceptor (34.6 % increase in J value) by shortening the binding distance ( r = 2.83 ± 0.01 nm), thereby elevating energy transfer efficiency ( E = 34.65 %). This study provides molecular-level insights into the biocompatibility and toxicity assessment of cobalt-based compounds.
研究领域:
无机化学
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生物无机化学
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分子毒理学
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物理化学
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药学
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高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2024
·
IF 5.4
Feng Yang,Yilan Wang,Huatao He,Guanxiong Wang,Menghan Yang,Meiying Hong,Jin Huang,Yaolei Wang
Water-in-water (W/W) Pickering emulsions , exhibit considerable potential in the food and pharmaceutical fields owing to their compartmentalization and high biocompatibility . However, constrained by the non-uniform distribution of shear forces during emulsification or the spatial obstruction in polydimethylsiloxane (PDMS) passive microfluidic platform , the existing methods cannot generate monodisperse W/W Pickering emulsions with high particle coverage rate, thereby limiting their applications. Herein, a novel microfluidic system is designed for the preparation of monodisperse and highly particle-covered W/W Pickering emulsions under mild conditions. pH-responsive Polyethylene glycol (PEG)/phosphate aqueous two-phase system (ATPS) is used for the emulsions' preparation. Notably, a coverage rate of 96 ± 3 % is obtained by adjusting the length of the helical coiled tube, as well as the size and contact angle of genipin cross-linked BSA (BSA-GP) particles. Moreover, these W/W Pickering emulsions, with surfaces almost completely covered, can maintain monodisperse (N coal = 1.18 ± 0.03) for one day. Furthermore, the results of ranitidine hydrochloride (RH) release demonstrated that the drug release rate of W/W Pickering emulsions in the simulated gastric fluid (SGF) was 10 times faster than that in the neutral solution. We believe that the highly particle-covered monodisperse W/W Pickering emulsions possess great potential applications in bioencapsulation for foods and drug delivery.
研究领域:
化学工程
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材料科学
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药剂学
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食品科学
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DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Xinmeng Zhou,Huan Liu,Zilian Yu,Hao Yu,Decheng Meng,Liran Zhu,Huanjun Li
Hydrogel microneedle patches have emerged as promising platforms for painless, minimally invasive, safe, and portable transdermal drug administration. However, the conventional mold-based fabrication processes and inherent single-functionality of such microneedles present significant hurdles to broader implementation. Herein, we have developed a novel approach utilizing a precursor solution of robust nanocomposite hydrogels to formulate photo-printable inks suitable for the direct 3D printing of high-precision, triple-responsive hydrogel microneedle patches through digital light processing (DLP) technology. The ink formulation comprises four functionally diverse monomers including 2-(dimethylamino)ethyl methacrylate , N -isopropylacrylamide, acrylic acid , and acrylamide , which were crosslinked by aluminum hydroxide nanoparticles (AH NPs) acting as both reinforcing agents and crosslinking centers. This results in the formation of a nanocomposite hydrogel characterized by exceptional mechanical strength , an essential attribute for the 3D printing of hydrogel microneeedle patches. Furthermore, this innovative 3D printing strategy facilitates facile customization of microneedle geometry and patch dimensions. As a proof-of-concept, we employed the fabricated hydrogel microneedles for transdermal delivery of bovine serum albumin (BSA). Importantly, these hydrogel microneedles displayed no cytotoxic effects and exhibited triple sensitivity to pH, temperature and glucose levels, thereby enabling more precise on-demand drug delivery. This study provides a universal method for the rapid fabrication of hydrogel microneedles with smart responsiveness for transdermal drug delivery applications.
研究领域:
材料科学
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生物医学工程
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药学
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纳米技术
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DOI
PubMed
高影响因子
Food & Function
·
2024
·
IF 5.1
Gang Yu,Jianxin He,Zhongshan Gao,Linglin Fu,Qiaozhi Zhang
Advanced glycation end-products (AGEs) are complex and heterogeneous compounds widely present in processed foods. Previous studies evidenced the adverse effects of AGEs on gut homeostasis, but the precise pathological mechanisms and molecular pathways responsible for the disruption of intestinal barrier integrity by AGEs remain incompletely elucidated. In this study, protein-bound AGEs (BSA-MGO), the most common type of dietary AGEs, were prepared by methylglyoxal-mediated glycation, and an in vitro human epithelial colorectal adenocarcinoma (Caco-2) cell model was employed to evaluate the impact of protein-bound AGEs on gut epithelial function. Results showed that exposure to BSA-MGO significantly increased the permeability of Caco-2 cell monolayers as evidenced by the decreased transepithelial electrical resistance value, increased paracellular transport of FITC-dextran, and down-regulated tight-junction proteins. Parallelly, BSA-MGO induced pro-inflammatory responses and oxidative stress in the monolayers. Transcriptomic profiling further revealed that BSA-MGO disrupted the retinol metabolism, thereby contributing to the barrier integrity damage in epithelial cells. Overall, these results provide valuable insights into the disrupting effects of dietary AGEs on intestinal barrier function, and the perturbed pathways present potential targets for further exploration of the molecular mechanisms underlying the detrimental effect of processed foods on gut health.
研究领域:
胃肠病学
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细胞生物学
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分子生物学
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营养学
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炎症
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Dan Yang,Yao Zhou,Mengqi Wang,Ying Yuan,Yuqi Feng,Yapeng Fang,Guoliang Li,Dechun Liu,Xiaolin Yao
Biomacromolecules in physiological environments would adsorb onto the nanoparticles (NPs) to form corona layers, in which protein coronas (PCs) are the major constituent. PCs always play diverse influences on the fate of NPs in vitro and in vivo, especially for active-targeting NPs (e.g., transferrin-modified nanoparticles, Tf-NP). In order to eliminate the inhibition of PCs on the efficiency of Tf-NP, the precoated Tf-NP with bovine serum albumin (BSA, B@Tf-NP) was designed to fabricate an “active PCs” (PCs formed by artificial modification) against the “passive PCs” (PCs formed in the biological environments), which was inspired by the formation pattern of PCs. The results indicated that B@Tf-NP had similar particle size, dispersion, and physical stability with Tf-NP. Surprisingly, B@Tf-NP enhanced the cellular uptake in enterocytes and permeability in intestinal tract of mice. Notably, the concentration ratio of BSA to Tf that could ensure Tf revealed timely during the interacted process was considered to be appropriate. These findings provide an easy while efficient design platform for active-targeting NPs to overcome the biomacromolecular barrier in oral administration.
研究领域:
纳米技术
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药学
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生物医学工程
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
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2021
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IF 8.742
Qiang Xue,Kaisong Zhang
Current membranes have limitations in permeability and selectivity, and require long-time stability, which drive the development of high-performance nanofiltration (NF) membranes. MXene is a new type of highly hydrophilic and negatively charged two-dimensional (2D) nanosheets material, especially its supernatant has good dispersibility and stability. The MXene supernatant containing several layers was treated as aqueous solvent for interfacial polymerization, consequently the prepared MXene nanocomposite membrane could maintain high permeselectivity even under low pressures required for low carbon. The characterization results showed that the MXene nanocomposite membrane had better hydrophilicity and higher negative charge. At the same time, the Na 2 SO 4 rejection was increased to 97.6% and the pure water flux was improved by about 1.7 times compared with the pristine membrane. In the BSA antifouling test and 58-day persistent desalination test, the MXene nanocomposite membrane showed the better antifouling property, high rejection and good stability. As an excellent 2D modifier, MXene provided fresh ideas and guidance for low-carbon and persistent NF membranes with excellent separation performance.
研究领域:
材料科学
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环境工程
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纳米技术
·
水处理
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DOI
高影响因子
Acta Biomaterialia
·
2024
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IF 9.4
Meng Zhang,Yiqing Ye,Zhengyun Chen,Xiaodong Wu,Yue Chen,Pengfei Zhao,Mengdan Zhao,Caihong Zheng
Endometriosis seriously affects 6–10% of reproductive women globally and poses significant clinical challenges. The process of ectopic endometrial cell colonization shares similarities with cancer, and a dysfunctional immune microenvironment, characterized by non-classically polarized macrophages, plays a critical role in the progression of endometriosis. In this study, a targeted nano delivery system (BSA@Mif NPs) was developed using bovine serum albumin (BSA) as the carrier of mifepristone. The BSA@Mif NPs were utilized to selectively target M2 macrophages highly enriched in ectopic endometrial tissue via the SPARC receptor. This targeting strategy increases drug concentration at ectopic lesions while minimizing its distribution to normal tissue, thereby reducing side effects. In vitro studies demonstrated that BSA@Mif NPs not only enhanced the cellular uptake of M2-type macrophages and ectopic endometrial cells but also improved the cytotoxic effect of mifepristone on ectopic endometrial cells. Furthermore, the BSA@Mif NPs effectively induced immunogenic cell death (ICD) in ectopic endometrial cells and repolarized M2-type macrophages toward the M1 phenotype, resulting in a synergistic inhibition of ectopic endometrial cell growth. In vivo experiments revealed that BSA@Mif NPs exhibited significant therapeutic efficacy in endometriosis-bearing mice by increasing drug accumulation in the endometriotic tissues and modulating the immune microenvironment. This targeted biomimetic delivery strategy presents a promising approach for the development of endometriosis-specific therapies based on existing drugs. Statement of Significance Macrophages play an essential role in immune dysfunctional microenvironment promoting the occurrence and progression of endometriosis and can be a crucial target for developing immune microenvironment regulation strategies for the unmet long-term management of endometriosis. The albumin nanoparticles constructed based on SPARC overexpression in macrophages and endometrial cells and albumin biosafety can achieve the targeted therapy of endometriosis by increasing the passive- and active-mediated drug accumulation in ectopic endometrium and remodeling the immune microenvironment based on macrophage regulation. This study has the following implications: i) overcoming the inherent shortcomings of clinical drugs by nanotechnology is an alternative way of developing medication; ii) developing microenvironment modulation strategies based on macrophage regulation for endometriosis management is feasible.
研究领域:
妇科学
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药学
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纳米技术
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免疫学
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DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Jingke Yao,Xunjie Shang,Jiayi Wei,Liting Guo,Feifan Sheng,Nannan Shao,Limin Chen,Zhe Zhang,Yunlong Zhou,Wei Chen
Biofilm and multidrug-resistant bacteria limit therapy effects on bacteria keratitis. Hence, we developed an all-in-one strategy based on a photothermal trigger to combat multidrug-resistant bacteria and eradicate biofilm. In the nanoplatforms, BSA-Ag2Te nanoparticles (NPs) fabricated by a one-step synthesis approach were utilized as photothermal agents to generate low temperature (40–48 °C) and then modified with RGD (Arg-Gly-Asp) to increase the drug concentration and retention in the cornea. The heat generated from photothermal therapy can accelerate the Ag+ release. In addition, laser-triggered release of Ag+ can significantly increase the ROS content in the bacteria. This synergistic approach could effectively eradicate multidrug-resistant bacteria and biofilm by photothermal therapy (PPT) and the combination of Ag+ and ROS. Based on the synergistic treatment of PTT, Ag+, and ROS, BSA-Ag2Te@RGD NPs exhibited outstanding antibacterial effects on a mice MRSA-infected keratitis model. BSA-Ag2Te@RGD NPs will be promising candidates for applications of low-temperature PTT in bacterial keratitis treatments.
研究领域:
纳米技术
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生物医学工程
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眼科学
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药学
·
细菌学
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2019
·Vol. 43
·期 6
·2694-2701
·
IF 3.069
Yuan Zhang,Yang Xing,Ming Xian,Shaomin Shuang,Chuan Dong
Herein, distinctive nanomaterial-based mesoporous silica nanoparticles (MSNs) successively coupled with bovine serum albumin (BSA) and folic acid (FA) were engineered for targeting the release of epirubicin (EPI). The BSA molecule as a “gatekeeper” prevents untimely drug leakage until the BSA coating layer is biodegraded in response to GSH. FA promotes the specific intracellular delivery of the drug to high folate receptor (FR)-expressing cancer cells to improve the efficacy of chemotherapy. In vitro drug release profiles show that this drug carrier can avoid premature release of the drug under physiological conditions, and redox-responsive EPI release from EPI/MSN-FA with easy biodegradability is significantly accelerated in the presence of GSH due to the cleavage of the intramolecular disulfide bond; thus, a “redox-triggered release” of the drug in the simulated targeted tissue microenvironment is achieved. The folate-mediated cellular uptake measured by confocal laser scanning microscopy (CLSM) shows that the hybrid nanoparticles more likely enter the FR-positive (FR+) HepG2 cells than the FR-negative (FR−) PC-12 cells. Toxicity experiment also demonstrates the negligible toxicity of MSN-FA and an increase in the antitumor suppression of EPI/MSN-FA with an increase in the dosage. The results show that the strategy of combining mesoporous silica with protein molecules having stimuli-responsive biodegradability will offer new potential directions towards the “on-demand” drug release for pinpoint chemotherapy in cancer treatment.
研究领域:
纳米技术
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药学
·
癌症研究
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药物递送系统
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生物医学工程
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高影响因子
RSC Advances
·
2018
·Vol. 8
·期 46
·26029-26035
·
IF 2.936
Ming Li,Yuanyuan Zhang,Rujin Zhao,Zhenjiang Liu,Xia Hong,Yin Cui,Yonglai Xue,Daolin Du
The potential homogeneous assay employing immunomagnetic beads (IMB) has been receiving attention as a screening tool in food-safety control; the method is simple, efficient, and does not require long incubation times or complex separation steps. In this study, a homogeneous immunoassay has been successfully developed and applied in the determination of aflatoxin B1 (AFB1) contamination in agricultural products by coupling IMB and the biotin-streptavidin (BSA) (BSA-IMB) system. Under optimal conditions, the limit of detection (LOD, IC10), half-maximal inhibition concentration (IC50) and detection range (IC20–IC80) of BSA-IMB are 0.00579, 0.573 and 0.0183–17.9 ng mL−1, respectively, for AFB1. The detection of AFB1 by BSA-IMB can be achieved in 40 min (ELISA needs at least 180 min). The cross-reactivities of BSA-IMB with its analogues are negligible (<3.82%); these results indicate high selectivity. The spiked recoveries are in the range from 89.6 to 118.2% with relative standard deviations (RSDs) of 3.4 to 13.2% for AFB1 in agricultural product samples. Furthermore, the results of BSA-IMB for authentic samples show reliability and high correlation of 0.9928 with an HPLC-fluorescence detector. The proposed BSA-IMB system is demonstrated to be a satisfactory tool for homogeneous, efficient, sensitive, and alternative detection of AFB1 in a wide detection range for agricultural product samples.
研究领域:
食品安全
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分析化学
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毒理学
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农业科学
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Jing Zhang,Di Zhang,Qian Li,Yue Jiang,Aixin Song,Zhonghao Li,Yuxia Luan
Potentiating systemic immunity against breast cancer is in the most urgent demand as breast cancer is less sensitive to immune checkpoint blockade. Although phototherapy and some chemotherapy can trigger immunogenic cell death (ICD) for T cell-mediated antitumor immune response, their immunotherapy efficacy is severely restricted by insufficient phototherapeutic capability and severe multidrug resistance (MDR). Inspired by both the hypersensitivity to phototherapy and the key role of MDR for mitochondria, a rationally engineered immunity amplifier via mitochondria-targeted photochemotherapeutic nanoparticles was, for the first time, achieved to fight against low-immunogenic breast cancer without additional immune agents. The newly synthesized task-specific mitochondria-targeted IR780 derivative (T780) was integrated with chemotherapeutic doxorubicin (DOX) to form multifunctional nanoparticles via an assembling strategy along with bovine serum albumin (BSA) as a biomimetic corona (BSA@T780/DOX NPs). The in situ enhancement in both phototherapy and MDR reversal by targeting mitochondria with BSA@T780/DOX NPs boosted highly efficient ICD toward excellent antitumor immune response. The newly developed strategy not only eradicated the primary tumor but also eliminated the bilateral tumors efficiently, as well as preventing metastasis and postsurgical recurrence, demonstrating great interest for fighting against low-immunogenic breast cancer.
研究领域:
癌症研究
·
纳米技术
·
免疫疗法
·
药物科学
·
生物医学工程
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）