Materials
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2014
·Vol. 7
·期 4
·2459-2473
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IF 1.879
Hu-Fan Song,Ai-Zheng Chen,Shi-Bin Wang,Yong-Qiang Kang,Shi-Fu Ye,Yuan-Gang Liu,Wen-Guo Wu
Using ammonium bicarbonate (AB) particles as a porogen, chitosan (CS)-based hemostatic porous sponges were prepared in supercritical carbon dioxide due to its low viscosity, small surface tension, and good compatibility with organic solvent. Fourier transform infrared spectroscopy (FTIR) spectra demonstrated that the chemical compositions of CS and poly-(methyl vinyl ether-co-maleic anhydride) (PVM/MA) were not altered during the phase inversion process. The morphology and structure of the sponge after the supercritical fluid (SCF) process were observed by scanning electron microscopy (SEM). The resulting hemostatic sponges showed a relatively high porosity (about 80%) with a controllable pore size ranging from 0.1 to 200 μm. The concentration of PVM/MA had no significant influence on the porosity of the sponges. Comparative experiments on biological assessment and hemostatic effect between the resulting sponges and Avitene®were also carried out. With the incorporation of PVM/MA into the CS-based sponges, the water absorption rate of the sponges increased significantly, and the CS-PVM/MA sponges showed a similar water absorption rate (about 90%) to that of Avitene®. The results of the whole blood clotting experiment and animal experiment also demonstrated that the clotting ability of the CS-PVM/MA sponges was similar to that of Avitene®. All these results elementarily verified that the sponges prepared in this study were suitable for hemostasis and demonstrated the feasibility of using SCF-assisted phase inversion technology to produce hemostatic porous sponges.Keywords:chitosan;hemostatic sponge;porous structure;supercritical fluids
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CARBOHYDRATE POLYMERS
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2014
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IF 3.916
Na Wang,Xianfeng Wang,Yongtang Jia,Xiaoqi Li,Jianyong Yu,Bin Ding
Here we describe a formaldehyde sensor fabricated by coating polyethyleneimine (PEI) functionalized chitosan nanofiber-net-binary structured layer on quartz crystal microbalance (QCM). The chitosan fibrous substrate comprising nanofibers and spider-web-like nano-nets constructed by a facile electro-spinning/netting process provided an ideal structure for the uniform PEI modification and sensing performance enhancement. Benefiting from the fascinating nanostructure, abundant primary amine groups of PEI, and strong adhesive force to the QCM electrode of PEI–chitosan membranes, the developed formaldehyde sensor presented rapid response and low detection limit (5 ppm) at room temperature. These findings have important implications in fabricating multi-dimensional nanostructures on QCM for gas sensing and chemical analysis.
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JOURNAL OF APPLIED POLYMER SCIENCE
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2014
·Vol. 132
·期 15
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IF 1.64
Qian Ma,Yanfei Liu,Zhe Dong,Jianglang Wang,Xin Hou
Hydrophobic and nanoporous chitosan–silica composite aerogels with low density, high porosity, and superior oil absorbency were successfully prepared by a typical sol–gel method and a two-step hydrophobic treatment. The morphologies, porosity characteristics, mechanical properties, thermal stability, hydrophobicity, and oil absorbencies of the composite aerogels were systematically investigated. The nitrogen physisorption analysis showed that composite aerogels had large specific surface areas and uniform nanoporous structures. In addition, the composite aerogels could support 7000 times its own weight; this indicated the role of the supporting skeleton played by chitosan. The hydrophobicity and lipophilicity was demonstrated with a water contact angle of 137° and an oil contact angle of 0°. Importantly, the composite aerogel with 20 wt % chitosan had a relatively high oil absorbency of 30 g/g and could be reused up to 10 times. Therefore, the chitosan–silica composite aerogels in this study had a broad prospect to be used as efficient and recyclable oil absorbents. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132 , 41770.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2015
·
IF 2.858
Qin Zou,Junfeng Li,Yubao Li
Chitosan microspheres with diameter of 14.3–48.5 μm were prepared by emulsion method and using natural vanillin as cross-linking agent. The surface morphology and microstructure of the microspheres were characterized by scanning electron microscopy, X-ray diffraction and Fourier-transform infrared spectroscopy, etc. The hollow microspheres showed a well-defined spherical shape with median diameter of 30.3 μm and possessed a uniform surface with micro-wrinkles, which is in favor of the drug release. Interpenetrating network cross-linking mechanism might result from the Schiff base reaction and the acetalization of hydroxyl and carbonyl between chitosan and vanillin. Berberine, as a model drug, was loaded in the microspheres and released in a sustainable manner. The drug loading ratio could change from 9.16% to 29.70% corresponding to the entrapment efficiency of 91.61% to 74.25%. In vitro cell culture study using MG63 cells and in vivo implantation clearly showed that the microspheres could provide favorable cell attachment and biocompatibility. The results confirm that the drug-loaded vanillin-crosslinked chitosan microspheres could be a worthy candidate either as carriers of drugs and cells, or as therapeutic matrix for bone repair and regeneration.
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INDUSTRIAL CROPS AND PRODUCTS
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2015
·
IF 2.837
Shan Lin,Lihui Chen,Liulian Huang,Shilin Cao,Xiaolin Luo,Kai Liu
Cellulose-based membranes have emerged as an attractive alternative to non-biodegradable petrochemical materials. An important drawback, however, is that cellulose-based membranes are prone to biofouling. Silver nanoparticles (AgNPs) encapped with polyacrylic acid were conjugated with the chitosan/cellulose composite films to enhance the antimicrobial activities. Using the 1-ethyl-3-(3-dimethylaminopropyl) carbodii-mide hydrochloride and N -hydroxysuccinimide as biocoupling agents, AgNPs with an average size of 9 nm were distributed evenly in the film without agglomeration. The presence of AgNPs in the chitosan/cellulose–AgNPs composite films was further confirmed by X-ray diffraction measurements. Fourier transform infrared spectroscopy analysis supported the presence of amide bonds between the primary amino groups of chitosan and the carboxylic residues of coordination to silver nanoparticles. The antimicrobial properties of the chitosan/cellulose and chitosan/cellulose–AgNPs composite films were determined using the disk diffusion tests with Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ). As compared to the chitosan/cellulose composite films, the chitosan/cellulose–AgNPs composite films showed significantly improved antimicrobial activities.
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JOURNAL OF APPLIED POLYMER SCIENCE
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2017
·Vol. 134
·期 14
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IF 1.86
Hongjiao Wang,Shunli Liu,Aokai Zhang,Kewen Li,Olayinka Oderinde,Fang Yao,Guodong Fu
A series of N-succinyl Chitosan (NSCS) hydrogel derived from different substitution degree of NSCS were fabricated through the zinc ions crosslinking. Subsequently, a light-weight, transparent, and hierarchical NSCS-Zn(II) film was obtained by drying the hydrogel. The hierarchical structure of NSCS-Zn(II) film was confirmed by scanning electron microscopy. The NSCS-Zn(II) film exhibited excellent mechanical strength because of the preferential formation of the alignment structure. The tensile strength and Young's modulus reaches 119.3 MPa and 2.67 GPa when the NSCS substitution degree was 91.95%. Furthermore, a remarkable and enhanced broad-spectrum bactericidal properties of the NSCS-Zn(II) film was revealed through E. coli and S. aureus exposure, respectively. These features indicated that NSCS-Zn(II) film is a promising material that can be used in biomedical engineering. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017 , 134 , 44664.
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Biomedical Materials
·
2024
·Vol. 19
·期 5
·055024
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IF 3.9
Zhan Zhang,Xinzhe Zhao,Ziyu Song,Lu Wang,Jing Gao
The protein-polysaccharide nanofibers have attracted intensive attention in promoting wound healing, due to their components and nanoscale fibrous structure that mimics the native extracellular matrix (ECM). For the full-thickness wounds, in addition to promoting healing, hemostatic property and antibacterial activity are also of critical importance. However, currently, protein-polysaccharide-based nanofiber membranes exhibit poor mechanical properties, lack inherent hemostatic and antibacterial capabilities, as well as the ability to promote tissue repair. In this study, we developed composited membranes, which were composed of collagen (Col) and chitosan (Chs), through solvent alteration and post-processing, the membranes showed enhanced stability under physiological conditions, proper hydrophilic performance and improved mechanical property. Appropriated porosity and water vapor transmission rate, which benefit to wound healing, were detected among all the membranes except for Col membrane. Aimed at wound dressing, hemocompatibility, antibacterial activity and cell proliferation of the electrospun membranes were evaluated. The results indicated that the Col/Chs composited membranes exhibited superior blood clotting capacity, and the membranes with Chs exceeding 60% possessed sufficient antibacterial activity. Moreover, compared with Chs nanofibers, significant increase in cell grow was detected in Col/Chs (1:3) membrane. Taken together, the electrospun membrane with multiple properties favorable to wound healing, superior blood coagulation, sufficient antibacterial performance and promoting cell proliferation property make it favorable candidate for full-thickness skin wound healing.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Zhicheng Liu,Hao Zhang,Huining Su,Yang Chen,Xunan Jing,Daquan Wang,Shaohui Li,Hao Guan,Lingjie Meng
Developing porous hemostatic sponges that are both biosafe and multifunctional remains a complex challenge. Conventional hemostatic techniques often fall short in managing bleeding effectively, leading to severe critical cases such as suboptimal hemostasis, increased infection risk, and complications arising from profuse bleeding. To address these deficits, our study introduces a novel multifunctional nanocomposite sponge that synergistically incorporates chitosan (CS), cellulose (Cel), graphene oxide (GO), and silver (Ag) nanoparticles. The resulting CS/Cel/GO/Ag developed demonstrates a swelling rate exceeding 3000 %, an absorption rate of over 2100 %, and the lowest stress surpassing 20 kPa at an initial 80 % strain. In vitro analyses reveal that the CS/Cel/GO/Ag sponge has excellent cytocompatibility, non-hemolytic nature, and competence in blood cell adherence and bacterial inhibition. In vivo evaluations further demonstrate that compared to conventional hemostatic methods, the sponge substantially enhances hemostatic efficacy, as evidenced by the marked reductions in clotting times and diminished blood loss compared to conventional hemostatic methods. Specifically, the test results of the CS/Cel/GO/Ag sponge across three different models are as follows: for the rat tail amputation model, the clotting time was 99 s, while blood loss was 222 mg; for the rat liver injury model, the clotting time was 129 s. while blood loss was 812 mg; for the rat femoral artery laceration model, the clotting time was 96 s, while blood loss was 758 mg. The compelling attributes of the CS/Cel/GO/Ag sponges position them as a promising solution for the acute management of bleeding. Their excellent performance indicates they have potential role to play in trauma care.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Zhiwei Wen,Shuiling Qin,Huajie Huang,Xingle Xia,Wei Zhang,Wei Wu
Anthracyclines belong to a class of anti-tumor antibiotics, and their severe cardiotoxicity significantly limits their clinical use. Exosomes play key roles in intercellular communication, characterized by high biocompatibility and specific tissue and organ homing effects. In this study, doxorubicin, an anthracycline anticancer drug widely used in clinical chemotherapy, was selected as a model drug. To address the significant cardiotoxicity associated with doxorubicin, tumor exosomes are utilized as drug carriers. The homing effect of autologous exosomes enhances drug uptake by tumor cells and reduces cardiotoxicity. To enhance the stability of exosomes, improve therapeutic effectiveness, and reduce toxic side effects, chitosan was utilized to modify the surface of exosomes. Chitosan has a specific anti-tumor effect because it can target the CD44 receptor of tumor stem cells and interact with tumor cells through charge adsorption. Through in vitro cell experiments, in vivo pharmacokinetic experiments, and an in situ ectopic nude mouse tumor model, the study demonstrated that chitosan-modified tumor exosomes significantly alleviated the severe cardiotoxicity of doxorubicin, while also showing remarkable anti-tumor efficacy. This study introduces a novel approach to reduce the adverse side effects of anthracycline chemotherapeutic drugs and presents a highly promising nanocarrier delivery system.
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ACS Biomaterials Science & Engineering
·
2024
·
IF 5.4
Shasha Zhou,Jibing He,Quan Liu,Ting Chen,Xiangheng Guan,Haihan Gao,Jia Jiang,Jiaxing Wang,Xiaochun Peng,Jinglei Wu
Itaconic acid and its derivative 4-octyl itaconate (OI) represent a novel anti-inflammatory medication that has demonstrated efficacy in multiple inflammation models because of its minimal side effects. Recently, natural polymers conjugated with small molecule drugs, known as polymer-drug conjugates (PDCs), have emerged as a promising approach to sustained drug release. In this work, we reported an approach to prepare a PDC containing an OI and make it into an injectable hydrogel. Chitosan (CS) was selected for PDC synthesis because of its abundant free amino groups that can be conjugated with molecules containing carboxyl groups by carbodiimide chemistry. We used an ethanol/water cosolvent system to synthesize a CS-OI conjugate via EDC/NHS catalysis. The CS-OI conjugate had improved water solubility and unique anti-inflammatory activity and did not show compromised antibacterial activity compared with unmodified CS. Beta-glycerophosphate (β-GP) cross-linked CS-OI hydrogel exhibited good injectability with sustainable OI release and effectively modulated inflammatory response in a rat model. Therefore, this study provides valuable insights into the design of PDC hydrogels with inflammatory modulatory properties.
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FOOD CHEMISTRY
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2024
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IF 8.5
Bodan Luo,Simin Xuan,Xiaotong Wang,Keying Ding,Peng Jin,Yonghua Zheng,Zhengguo Wu
Chitosan is an ideal coating film for food preservation, but the performance of a single chitosan coating film is not good. Herein, the liposome was prepared by embedding copper nanoparticles (CuNPs) and thyme essential oil (TEO) in the hydrophilic and hydrophobic double-domain structure formed by phospholipids, and combining with chitosan to obtain a chitosan-based coating film for litchi preservation. The liposome was well-dispersed and stable with an average particle size of about 190 nm. The liposome showed excellent controllable release properties, and the cumulative release rate of TEO was 65.17 % and that of CuNPs was 15.17 % after 7 days. Furthermore, the oxygen and water vapor barrier properties of the coating film were greatly improved. Importantly, the film possessed effective antioxidant, antibacterial activity and excellent safety, which presents a better fresh-keeping effect on litchi. This study provides insights into the design and manufacture of food packaging for controllable and long-lasting preservation.
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ACS Applied Materials & Interfaces
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2024
·
IF 8.3
Yuting Li,Chen Yang,Feng Hu,Hui Hu,Yuncheng Xu,Hongbing Deng,Yumin Du,Xiaowen Shi
The storage of dynamic information in hydrogels has aroused considerable interest regarding the multiple responsiveness of soft matter. Herein, we propose an electrical writing methodology to prepare dopamine (DA)-modified chitosan hydrogels with a dynamic information storage ability. A pH-responsive chitosan hydrogel medium was patterned by cathodic writing to in situ generate OH– in the writing area, at which dopamine underwent an auto-oxidation reaction in the locally alkaline environment to generate a dark color. The patterned information on the hydrogel can be encoded simply by electrical signals. The speed of information retrieval is positively correlated with the charge transfer during the electrical writing process, and the hiding of information is negatively correlated with the environmental stimulus (i.e., dopamine concentration, pH value, etc.). To showcase the versatility of this medium for information storage and the precision of the pattern, a quick response (QR) code is electronically written on dopamine-modified chitosan hydrogel and can be recognized programmably by a standard mobile phone. The results show that electrical regulation is a novel means to program the information storage process of hydrogels, which inspires future research on structural and functional information storage using stimulus-responsive hydrogels.
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