Analytical Methods
·
2015
·Vol. 7
·期 6
·2406-2412
·
IF 1.821
Weiwen Hu,Qiong Hu,Lianzhi Li,Jinming Kong,Xueji Zhang
In this work, an efficient method for the sequence-specific detection of DNA based on a morpholino-functionalized silicon chip platform has been proposed. Briefly, morpholino was first immobilized on the surface of a silicon chip using 3-aminopropyltriethoxysilane (APTES) as the silane coupling agent and 1,4-phenylenediisothiocyanate (PDITC) as the cross-linker and then hybridized with DNA in the ensuing step. The fluorescence label was introduced by strongly binding Rhodamine B, which contains a terminal carboxylic group, with DNA by means of phosphate–zirconium–carboxylate coordination reaction. X-ray photoelectron spectroscopy (XPS) was used to characterize the silicon surface. Under optimal conditions, the morpholino-functionalized silicon chip presented a great linear relationship between the fluorescence intensity and the logarithm of target DNA concentrations in the range from 1 pM to 1 nM with a detection limit of 4.52 pM. Furthermore, fully complementary versus single-base mismatched, three-base mismatched and non-complementary DNA could be effectively identified. The chip showed excellent stability because it could be reused for another hybridization experiment after denaturing the morpholino–complementary DNA duplex. In addition, the chip rendered satisfactory analytical performance for the detection of DNA in serum samples, thus exhibiting practical significance. Morpholino-functionalized silicon chips display good sensitivity and selectivity for the detection of DNA and promising applications in single-nucleotide polymorphisms (SNPs).
研究领域:
生物化学
·
分子生物学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FLUORESCENCE
·
2023
·
IF 2.7
Yuan Xinyu,Zhang Xiufeng,Hu Jinshan,Zhang Buyue,Ma Xiaoying,He Jinxing,Shi Lei
In this report, we developed a sensing strategy based on ThT-E (a ThT derivative) and DNA G-quadruplex for the label-free detection of Zn 2+ . In the absence of Zn 2+ , there was a fluorescence enhancement of ThT-E by interaction with human telomere sequence. On the addition of Zn 2+ , Zn 2+ induced a more compact antiparallel G-quadruplex to release ThT-E, resulting in fluorescence quenching. The detection limit was 0.6996 μM, and the fluorescence intensity showed a good linear relationship with the concentration of Zn 2+ in the range of 0-10 μM. This sensing strategy which only needs to mix two kinds of materials has the characteristics of label-feel, simple operation, short response time, economical and efficient.
研究领域:
分析化学
·
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yanlin Zheng,Fei Li,Xiaohui Zhu,Min Wang,Yuantao Wen,Tao Zeng,Wukun Zhong,Chao Xing,Chunhua Lu
Photodynamic therapy (PDT) holds great promise for treating various types of cancer. However, its clinical advancement is hindered by the intrinsic hypoxic tumor microenvironment, which reduces PDT efficacy, and safety concerns related to the biological specificity of photosensitizers. Here we constructed a MnO 2 nanosheet based DNA tetrahedron-photosensitizer (TMPyP4) nanoplatform (TPM), which integration with tumor targeting (aptamer), gene drugs (DNAzyme) and photosensitizer agents for enhanced PDT. Once the TPM entered cancer cells through aptamer targeting, the MnO 2 nanosheet reacts with endogenous acidic and H 2 O 2 to generate Mn 2+ and oxygen (O 2 ), thereby alleviating the tumor hypoxic. Subsequently, TMPyP4 further converts the generated O 2 into singlet oxygen ( 1 O 2 ) under near-infrared light, enhancing PDT efficacy both in vitro and in vivo. Specifically, the generated Mn 2+ activates the DNAzyme and accelerate tumor target mRNA cleavage, resulting in effective PDT-gene synergistic therapy for tumors. This work offers novel perspectives on advancing precise and highly effective gene-PDT systems.
研究领域:
生物医学工程
·
肿瘤学
·
纳米医学
✓ 已验证
产品关联
DOI
高影响因子
Matter
·
2024
·
IF 17.3
Yi Zhang,Weiwei Shi,Chao Ma,Sikang Wan,Cong Li,Jianlei Shen,Jingjing Li,Xiaoguo Liu,Dong Chen,Fan Wang,Hongjie Zhang,Chunhai Fan,Kai Liu
The impressive mechanical properties of DNA molecules play important physiological and pathological roles, which arise from their
intrinsically ordered and periodic double-helix structures. Nevertheless, bulk fabrication of DNA-based materials that inherit their nanoscale mechanical strength remains a grand challenge. Here, we
developed a multiscale engineering approach to assemble with
highly ordered DNA bulk fibers. We found that the involvement of
metal ions enabled tunable assembly and orientation of DNA-metal
ensembles ranging from molecules to nanobundles and to macroscale filaments. This strategy remarkably enhanced the mechanical
performance of DNA fibers with the optimal tensile strength and
toughness reaching to 513 MPa and 130 MJ m3
, respectively,
which outperform even partially protein- and polymer-based fibers.
We further demonstrated the high scalability of this method by
continuous spinning of 1,000 m of robust fibers with 50 mg DNA,
which shows great promise in translations of DNA-based materials
in mechanobiology, bioelectronics, and beyond.
研究领域:
生物物理学
·
材料科学
·
分子生物学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Yong Liu,Rui Wang,Yuhan Zhu,Ruizhen Li,Jingdong Zhang
A photoelectrochemical (PEC) sensing platform was designed for catechol determination using CdS quantum dots (QDs), DNA and pristine graphene (PGR) nanocomposite film-modified electrode. In such a composite film-based PEC sensor, CdS QDs served as the photoelectric conversion element which produced photocurrent signal under visible light illumination; and DNA was designed as the biological recognition element which improved the PEC response of sensor toward catechol due to the interaction between DNA and catechol. To improve the electron transfer of the composite film, PGR with high electrical conductivity was doped, which significantly amplified the photocurrent signal of sensor. Based on such a CdS-DNA-PGR composite film-modified electrode, catechol showed a linear PEC response proportional to its concentration from 1.0 × 10 −8 to 1.0 × 10 −6 mol L −1 . The detection limit (3S/N) was estimated to be 4.9 × 10 −9 mol L −1 . The developed PEC sensor was successfully applied to determine trace catechol in water samples.
研究领域:
分析化学
·
环境科学
·
生物传感技术
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Yunlei Zhou,Fei Li,Hanwen Wu,Yan Chen,Huanshun Yin,Shiyun Ai,Jun Wang
A selective and sensitive electrochemical aptasensing strategy was developed for kanamycin detection based on target-triggered single-strand DNA adsorption on MoS 2 nanosheets and enzymatic signal amplification. Firstly, a Y-shape double-stranded DNA (dsDNA) was designed containing probe DNA, assist DNA (labeled with biotin at its 3′-terminal) and aptamer DNA. Then, in the presence of kanamycin, aptamer DNA can be released from the Y-shape dsDNA structure due to its interaction with kanamycin to form stable conjugate, which will destroy the Y-shape dsDNA structure and lead to the presence of two-fragment of single-stranded DNA (ssDNA) on the remained half-hybrid DNA structure (containing probe DNA and assist DNA). Based on the strong interaction between ssDNA and MoS 2 nanosheets, the half-hybrid DNA structure can be captured on MoS 2 nanosheets modified electrode surface, which triggers the subsequent immobilization of streptavidin-alkaline phosphatase (SA-ALP) compound. Under the catalysis of ALP, p -nitrophenol phosphate (PNPP) is hydrolyzed to produce p -nitrophenol (PNP). Based on the linear relationship between the electrochemical oxidation signal of PNP and kanamycin concentration, kanamycin can be detected with the detection limit of 0.029 nM (S/N = 3). The aptamer-MoS 2 nanosheets based electrochemical biosensor shows good selectivity, reproducibility for kanamycin detection.
研究领域:
分析化学
·
生物传感器
·
电化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2010
·Vol. 168
·期 3
·347-354
·
IF 2.648
Qian Ping,Ai Shiyun,Yin Huanshun,Li Jinhuan
Au-Pd bimetallic nanoparticles were prepared in the presence of an amine-terminated dendrimer of a fourth generation poly(amidoamine) type. A biosensor was fabricated by immobilizing dsDNA on a thin layer of a dendrimer-encapsulated bimetallic nanoparticles (Au-Pd) in a chitosan composite on a glassy carbon electrode. The biosensor was evaluated by square wave voltammetry for the determination of the oxidative damage of immobilized DNA and the antioxidant capacity of sericin. The biosensor is shown to be suitable for the rapid detection of DNA damage and the assessment of the antioxidant capacity of sericin.
研究领域:
生物分析化学
·
纳米生物技术
·
分子毒理学
·
食品与药物分析
·
生物传感技术
✓ 已验证
DOI
高影响因子
Scientific Reports
·
2015
·Vol. 5
·期 1
·1-8
·
IF 5.578
Qu JieHao,Hou XianLiang,Fan WanChao,Xi GuangHui,Diao HongYan,Liu XiangDon
A facile strategy for fabricating scalable stamps has been developed using cross-linked polyacrylamide gel (PAMG) that controllably and precisely shrinks and swells with water content. Aligned patterns of natural DNA molecules were prepared by evaporative self-assembly on a PMMA substrate and were transferred to unsaturated polyester resin (UPR) to form a negative replica. The negative was used to pattern the linear structures onto the surface of water-swollen PAMG and the pattern sizes on the PAMG stamp were customized by adjusting the water content of the PAMG. As a result, consistent reproduction of DNA patterns could be achieved with feature sizes that can be controlled over the range of 40%–200% of the original pattern dimensions. This methodology is novel and may pave a new avenue for manufacturing stamp-based functional nanostructures in a simple and cost-effective manner on a large scale.
研究领域:
纳米技术
·
材料科学
·
生物工程
✓ 已验证
DOI
PubMed
高影响因子
Biomolecules
·
2025
·Vol. 15
·期 3
·315
·
IF 4.8
Dongxia Pan,Mukaddas Mijit,Hui Wang,Chaoqun Sun,Bantan Pingcuo,Zhixue Yu,Benhai Xiong,Xiangfang Tang
The A-to-G mutation (FecB) in theBMPR1Bgene is strongly linked to fertility in sheep, significantly increasing ovulation rates and litter sizes compared to wild-type populations. The rapid and reliable screening of theFecBgene is therefore critical for advancing sheep breeding programs. This study aimed to develop a fast and accurate method for detecting theFecBmutation and genotyping the gene to enhance sheep reproduction and productivity. To achieve this, we integrated the CRISPR-Cas12a system with an optimized amplification refractory mutation system (ARMS). A similar DNA origami technique-based fluorescence reporter nanotree structure was synthesized using gold nanomagnetic beads as carriers to amplify the fluorescence signal further. The resulting biosensing platform, termed CRISPR-ARMS, demonstrated excellent sensitivity for detectingFecBmutations, with a detection limit as low as 0.02 pmol. Therefore, this innovative approach shows great promise for single-base mutation detection and represents a pioneering tool for high-yield genetic screening.
研究领域:
分子生物学
·
遗传学
·
动物科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Bo-Xin Zheng,Wei Long,Yi-Han Zhang,Xuan-He Huang,Cui-Cui Chen,Dong-Xiao Zhong,Meng-Ting She,Ze-Xin Chen,Dong-Peng Cai,Yu-Jing Lu,Wing-Leung Wong
Red fluorescent G-quadruplex DNA selective organic binding probe is relatively underdeveloped. In the present study, we designed a series of new fluorescent probes that exhibit large Stokes shift (112−131 nm), high quantum yield (up to 0.56), robust against photo-bleaching and high selectivity towards quadruplex structures of c-MYC and telomere DNA with strong binding affinity . The discrimination ability of the probes towards G-quadruplex structures against single-/double-stranded DNA or RNA was investigated by analyzing their structural property with respect to their binding specificity. From the sensing application of the probe targeting pu27, an excellent linear relationship (R 2 = 0.9997) with respect to the pu27- A3 interaction signal was observed. The LOD was found down to 4.0 nM, which is superior to thiazole orange dye. The probe is able to realize real-time fluorescence visualization in aqueous, PAGE staining and live cell imaging. In addition, from the immunofluorescence analysis using the probe and BG4 antibody, the A3 foci and BG4 staining co-localized well in PC3 cells, which suggest that the probe is G-quadruplex selective and is useful for visualizing G-quadruplex DNA structures in live cells. Cytotoxicity assays also demonstrated that the probe has low cytotoxicity against both PC3 cancer cells and 16HBE normal cells.
研究领域:
生物化学
·
分子生物学
·
医学诊断
✓ 已验证
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Li Li Li,Wen Yi Lv,Yao Wang,Yuan Fang Li,Chun Mei Li,Cheng Zhi Huang
DNA logic nanodevices have prospects in molecular recognitions but still face challenges in achieving DNA computation-controlled regulation in specific compartments of living cells. By incorporating the i-motif sequence and ATP aptamers into a Y-shaped DNA (Y-DNA) structure, and applying gold nanoparticles (AuNPs) as the transporting carrier, herein we present a new type of DNA logic nanodevices to monitor the ATP levels in lysosomes of living cells. Triple energy transfers including dual fluorescent resonance energy transfers (FRETs) and a nanometal surface energy transfer (NSET) occurred in the DNA logic nanodevices. It was identified that the proposed nanodevices perform an AND logic operation to output FRET signals only when an endogenous proton and ATP simultaneously exist in the cellular microenvironment. Owing to the use of the i-motif sequence, the nanodevices have lysosome-recognizing capacity without causing alkalization of the acidic organelle, making DNA computation-controlled regulation at the level of cellular organelles achievable. These DNA logic nanodevices show high application prospects in lysosome-related cellular function and disease treatment.
研究领域:
分子生物学
·
纳米技术
·
细胞生物学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DNA AND CELL BIOLOGY
·
2025
·
IF 2.6
Lijiang Zhao,Xueqing Wu,Qiang Li,Yan Shen,Sheng Zeng,Jinbao Wang,Qian Liu
Bisphenol A (BPA) is the most common endocrine disruptor that has toxicity to the reproductive system and male infertility. However, the underlying mechanisms of BPA’s toxicity to Sertoli cells remain poorly understood. Cellular communication network factor 5 (CCN5) is reported to regulate cell proliferation, apoptosis, and differentiation. Our study demonstrated a significant elevation of CCN5 expression in the testis of nonobstructive azoospermia patients and TM4 Sertoli cells exposed to BPA. Knockdown of CCN5 reduced apoptotic cells after BPA treatment, as determined by flow cytometry and terminal deoxynucleotidyl transferase dUTP nick end labeling assays. Cells exposed to BPA showed increased expressions of Bax and cleaved poly(ADP-ribose) polymerase, decreased expression of Bcl-2, as well as elevated activities of caspase-3 and caspase-9 in BPA-induced TM4 cells, which were reversed by CCN5 inhibition. Loss of CCN5 declined phosphorylation of protein kinase R-like endoplasmic reticulum kinase and eukaryotic translation initiation factor 2A and decreased activating transcription factor 4 and C/EBP-homologous protein in BPA-treated cells. Furthermore, silencing CCN5 blocked BPA-induced phosphorylation of p38 and c-Jun N-terminal kinase (JNK). Administration of anisomycin, a mitogen-activated protein kinase (MAPK) activator, reversed the effects of CCN5 knockdown on BPA-induced endoplasmic reticulum (ER) stress and apoptosis. Taken together, CCN5 promotes apoptosis and ER stress in Sertoli cells exposed to BPA by activating the p38/JNK MAPK signaling pathway.
研究领域:
生殖生物学
·
毒理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）