SPECTROSCOPY LETTERS
·
2015
·
IF 0.852
Bo Zhang,Yanbin Su,Lei Chen,Zhuofu Wu,Fengrong Zhang,Yanwen Su,Gang Zhao,Zhengqiang Li
The characteristic changes in UV-visible and Raman spectra of lithocholic acid on E-2-Butenal for antiglioma are the maximum absorption peak of E-2-Butenal from 302 to 319 nm, the decreases of Raman signal of 1688 cm−1 and 1641 cm−1 in E-2-Butenal by lithocholic acid with the phenomenon of 1688 cm−1 splitting, and the disappearance of the band at 932 cm−1, which clearly displays the change of wagging in methyl. In the presence of mitochondrial protein deriving from glioma, the above mentioned effects of lithocholic acid on E-2-Butenal are more significant by Raman spectra detections. The molecular targets of lithocholic acid on E-2-Butenal are carbonyl group, conjugated double bonds, and methyl, respectively. The study concludes that the interaction between lithocholic acid and E-2-Butenal decreases the carbonyl group bond strength in E-2-Butenal and then changes the structure and function of E-2-Butenal. The internal cause of the interaction between lithocholic acid and E-2-Butenal leading to the change of the carbonyl group bond strength is the electronic delocalization near the carbonyl group bond in E-2-Butenal. Since E-2-Butenal derives from glioma in the process of lipid peroxidation of mitochondria, our investigation significantly contributes to understanding the mechanism of action of lithocholic acid as a potential antiglioma drug.
✓ 已验证
产品关联
DOI
高影响因子
ECS Journal of Solid State Science and Technology
·
2024
·Vol. 13
·期 3
·037005
·
IF 1.8
Ning Lu,Boyu Guo,Zhikang Rao,Juntao Chen,Ying Xu
Electrochemical impedance sensors are widely used for bacterial detection and interpreted by the equivalent circuit model (ECM) to evaluate the concentration changes on the electrode interface. However, this method is mainly based on prior models and focuses on frequency domain analysis which cannot precisely describe unknown dynamic processes and a fractional surface coverage of electrode. Distribution of Relaxation Times (DRT) method, as an alternative to interpret impedance spectra can be applied to solve the problem. In this research, two electrode schemes with different modification steps were designed to collect impedance data. An Ensemble Tree-SHAP model was then adopted to quantitatively analyze the impedance data processed by ECM-DRT method in time-frequency domain and applied for the detection of Escherichia coli (E.coli). The results showed that using a dataset integrated with time-frequency domain features significantly improved the accuracy of bacterial concentration prediction. As a result, the RMSE of untrained concentration with LightGBM model was 6.4\times 1{0}^{-4}\,log CFU ml−1, with the sensor detection limit about 2.69 log CFU ml−1. The research provided a tool combining time-frequency analysis for accurate prediction of bacterial concentration, which is crucial for evaluation of the antibacterial effect of long-term antibiotics on E.coli in future.
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL TOXICOLOGY
·
2024
·
IF 4.4
Ling-Zi Xia,Li-Lan Liu,Jun-Zhe Yue,Zhen-Yu Lu,Ru-Ya Deng,Xi He,Can-Can Li,Burong Hu,Hai-Tao Gao
Objective Phthalates (PEs) could cause reproductive harm to males. A mixture of three widely used PEs (MPEs) was used to investigate the ameliorative effects of zinc (Zn) and vitamin E (VE) against male reproductive toxicity. Methods Fifty male SD rats were randomly divided into five groups ( n = 10). Rats in MPEs group were orally treated with 160 mg/kg/d MPEs, while rats in MPEs combined Zn and/or VE groups were treated with 160 mg/kg/d MPEs plus 25 mg/kg/d Zn and/or 25 mg/kg/d VE. After intervention for 70 days, it's was measured of male reproductive organs' weight, histopathological observation of sperms and testes, serum hormones, PIWI proteins and steroidogenic proteins. Results Compared with control, anogenital distance, testes weight, epididymides weight, and sex hormones were significantly decreased, while the sperm malformation rate was markedly increased in MPEs group ( p < .05); the testicular tissues were injured in MPEs group with disordered and decreased spermatids, and arrested spermatogenesis. PIWIL1, PIWIL2, StAR, CYP11A1 and CYP19A1 were down-regulated in MPEs group ( p < .05). However, the alterations of these parameters were restored in MPEs combined Zn and/or VE groups ( p < .05). Conclusion Zn and/or VE improved steroid hormone metabolism, and inhibited MPEs' male reproductive toxicity.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED THERMAL ENGINEERING
·
2025
·
IF 6.9
Yanjun Sun,Haiqi Zheng,Bohao Yin,Xiaopo Wang,Maogang He
With global energy consumption surging and environmental concerns escalating, absorption refrigeration systems are receiving increasing attention. This study investigates the absorption behavior and thermodynamic performance of absorption refrigeration systems using trans -1-chloro-3,3,3-trifluoro-1-propene (R-1233zd(E)) paired with phosphonium-based ionic liquids (ILs), trihexyltetradecylphosphonium bis(trifluoromethylsulfonyl) amide ([P 66614 ][Tf 2 N]) and trihexyltetradecylphosphonium bis(2,4,4-trimethylpentyl) phosphinate ([P 66614 ][TMPP]). Experimental solubility measurements of R-1233zd(E) in the ILs were conducted using an isochoric saturation method across a temperature range of 293.15–343.15 K and pressures up to 0.61 MPa. The Non-Random Two-Liquid (NRTL) thermodynamic model was employed to correlate solubility data, yielding excellent agreement with deviations of less than 3.84 %. Results revealed that [P 66614 ][TMPP] exhibits higher solubility due to its branched alkyl chains, increased free volume, and favorable van der Waals interactions. Thermodynamic analyses were performed for single-effect (SARS), double-effect (DARS), and compression-assisted cycles (LC-SARS, single-effect cycle with a compressor added in the low-pressure side; HC-SARS, single-effect cycle with a compressor added in the high-pressure side; LC-DARS, double-effect cycle with a compressor added in the low-pressure side). It demonstrated that [P 66614 ][TMPP] exhibits a higher coefficient of performance (COP) compared to [P 66614 ][Tf 2 N]. Compression assistance reduced the minimum generation temperature and improved COP by leveraging electrical energy. Among all configurations, LC-DARS with a compression ratio of 1.9 demonstrated the optimal balance between COP (0.82) and thermal energy utilization efficiency. These findings highlight the potential of R-1233zd(E)/[P 66614 ][TMPP] as a sustainable working pair for absorption systems, particularly in low-grade heat recovery applications.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Yang Li,Jitong Wang,Ping Gong,Yuchao Feng,Ting Yao,Xing Gao,Wei Guo,Xue Chen,Jie Cui,Xia Fan
An automatic method that combines online solid-phase extraction (online SPE) and heart-cutting two-dimensional liquid chromatography (2D-LC) was developed to determine seven fat-soluble vitamins simultaneously and was validated in pet food. The saponified sample solution was injected directly into our combined online SPE and heart-cut 2D-LC system for enrichment, purification, separation, and quantification. Vitamin A and E isomers were analyzed using pentafluorophenyl (PFP) as the first column. Vitamins D 2 and D 3 were analyzed using C 18 as the second column. The results showed good linearity (R 2 > 0.9964) for the analytes and good reproducibility (RSD < 3 %, n = 3). The limits of quantification for vitamins A, D 2 , and D 3 , and α −, β-, γ-, and δ-E (calculated using S/N = 10:1) were 0.24, 0.09, 0.09, 2.4, 2.4, 2.4 and 2.4 μg/g, respectively. The spiked recoveries were from 80.6 to 104.0 %. The proposed method considerably simplifies the analytical workflow of standard methods for the analysis of fat-soluble vitamins and improves efficiency.
研究领域:
分析化学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Jingnan Chen,Xianli Fu,Guolong Yang,Wei Liu
Nature tocopherols are mainly produced from vegetable oil deodorized distillates. And development of simple and green extraction method is highly desirable for nature tocopherols separation. In this work, a green, rapid and mild (room temperature) solvent extraction method based on phenolic deep eutectic solvents (DES) has been developed to simultaneously extract three isomers of tocopherols (α-, γ-, δ-tocopherol) from soybean oil deodorizer distillate methyl esters (SODD-ME) for the first time. Tetrabutyl ammonium chloride-based DESs (TBAC-DESs) were used as extraction solvents. The effect of factors on the extraction efficiency of three individuals and total tocopherols was investigated. The optimal extraction conditions were determined as follows: DES composed of TBAC and 4-methylphenol (molar ratio of 1:1), DES/SODD-ME 6:1 (w/w), hexane/SODD-ME 1:1 (w/w) and vortex-assisted extraction of 1 min at room temperature. Under these conditions, the maximum extraction efficiency of total tocopherols was (97.5 ± 0.5)%. And the molecular structure of the phenolic DESs had significant effect on the extraction selectivity on α-, γ- and δ- tocopherol from SODD-ME. The underlying extraction mechanism was that the TBAC-phenol DESs combined the three tocopherols by both hydrogen bonding and π-π interaction.
研究领域:
食品科学与工程
·
绿色化学
✓ 已验证
产品关联
DOI
高影响因子
BIOTECHNOLOGY LETTERS
·
2018
·Vol. 40
·期 5
·837-845
·
IF 1.846
Hui Chang-Ye,Guo Yan,Yang Xue-Qin,Zhang Wen,Huang Xian-Qing
Objectives To improve the Pb 2+ biosorption capacity of the potential E. coli biosorbent, a putative Pb 2+ binding domain (PbBD) derived from PbrR was efficiently displayed on to the E. coli cell surface. Results The PbBD was obtained by truncating the N-terminal DNA-binding domain and C-terminal redundant amino acid residues of the Pb 2+ -sensing transcriptional factor PbrR. Whole-cell sorbents were constructed with the full-length PbrR and PbBD of PbrR genetically engineered onto the surface of E. coli cells using Lpp-OmpA as the anchor. Followed by a 1.71-fold higher display of PbBD than PbrR, the presence of PbBD on the surface of E. coli cells enabled a 1.92-fold higher Pb 2+ biosorption than that found in PbrR-displayed cells. Specific Pb 2+ binding via PbBD was the same as Pb 2+ binding via the full-length PbrR, with no observable decline even in the presence of Zn 2+ and Cd 2+ . Conclusions Since surface-engineered E. coli cells with PbBD increased the Pb 2+ binding capacity and did not affect the adsorption selectivity, this suggests that surface display of the metal binding domain derived from MerR-like proteins may be used for the bioremediation of specific toxic heavy metals.
研究领域:
微生物学
·
环境科学
·
生物技术
✓ 已验证
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2018
·Vol. 7
·期 3
·578-585
·
IF 5.976
Yibo Yang,Jize Liu,Jie Cao,Zehang Zhou,Xinxing Zhang
Despite the wide range of applications of flexible strain sensors, most of them use covalently crosslinked elastomers as a stretchable matrix, which may cause potential environmental problems via the disposal of worn-out devices. To mitigate this environmental threat, endowing sensors with self-repairability can be a simple and effective way to prolong their service lives. However, most current self-repairing sensors require complicated syntheses, in which hazardous solvents and reagents are always needed. Therefore, it remains challenging to develop an environment-friendly self-repairing sensor. Here, we report a sustainable self-repairing strain sensor based on naturally-derived supramolecular elastomer and green-synthesized silver nanofibers. This E-skin sensor features a high conductivity (≥104 S m−1), low percolation threshold (0.32%), high sensitivity (gauge factor ∼405.6) and desirable real-time (≤30 s) self-repairability after damage. This work may pave the way for the development of renewable, biocompatible and self-repairing next-generation wearable electronic devices.
研究领域:
材料科学
·
环境科学
·
生物医学工程
✓ 已验证
DOI
高影响因子
BRAZILIAN JOURNAL OF BIOLOGY
·
2021
·
IF 1.651
Huang Y.,Zhang N.-J.,Zhao Z.
As an important enzyme, xylanase is widely used in the food, pulp, and textile industry. Different applications of xylanase warrant specific conditions including temperature and pH. This study aimed to carry out sodium alginate beads as carrier to immobilize previous reported mutated xylanase from Neocallimastix patriciarum which expressed in E. coli, the activity of immobilization of mutated xylanase was elevated about 4% at pH 6 and 13% at 62 °C. Moreover, the immobilized mutated xylanase retained a greater proportion of its activity than the wide type in thermostability. These properties suggested that the immobilization of mutated xylanase has potential to apply in biobleaching industry.
研究领域:
酶工程
·
工业微生物学
·
生物技术
✓ 已验证
DOI
PubMed
高影响因子
Microbial Cell Factories
·
2021
·Vol. 20
·期 1
·1-13
·
IF 5.328
Zhang Yuyang,Chen Hongping,Zhang Yao,Yin Huifang,Zhou Chenyan,Wang Yan
Background Violaceins have attracted much attention as potential targets used in medicines, food additives, insecticides, cosmetics and textiles, but low productivity was the key factor to limit their large-scale applications. This work put forward a direct RBS engineering strategy to engineer the violacein biosynthetic gene cluster cloned from Chromobacterium violaceum ATCC 12,472 to efficiently improve the fermentation titers.Results Through four-rounds of engineering of the native RBSs within the violaceins biosynthetic operon vioABCDE, this work apparently broke through the rate-limiting steps of intermediates conversion, resulting in 2.41-fold improvement of violaceins production compared to the titers of the starting strain Escherichia coli BL21(DE3) (Vio12472). Furthermore, by optimizing the batch-fermentation parameters including temperature, concentration of IPTG inducer and fermentation time, the maximum yield of violaceins from (BCDE)m (tnaA−) reached 3269.7 µM at 2 mM tryptophan in the medium. Interestingly, rather than previous reported low temperature (20 ℃), we for the first time found the RBS engineered Escherichia coli strain (BCDE)m worked better at higher temperature (30 ℃ and 37 ℃), leading to a higher-level production of violaceins.Conclusion sTo our knowledge, this is the first time that a direct RBS engineering strategy is used for the biosynthesis of natural products, having the potential for a greater improvement of the product yields within tryptophan hyperproducers and simultaneously avoiding the costly low temperature cultivation for large-scale industrial production of violaciens. This direct RBS engineering strategy could also be easily and helpfully used in engineering the native RBSs of other larger and value-added natural product biosynthetic gene clusters by widely used site-specific mutagenesis methods represented by inverse PCR or CRISPR-Cas9 techniques to increase their fermentation titers in the future.
研究领域:
合成生物学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
NANOTECHNOLOGY
·
2020
·Vol. 31
·期 39
·395602
·
IF 3.551
Hongchao Geng,Ning Jiang,Chenyi Li,Xingqi Zhu,Yan Qiao,Qingyun Cai
A novel visible light-driven photocatalyst (represented as Mn-CdS/ZCISe/CIS/TiO2) for the passivation of E. coli was prepared with TiO2 nanowires as support and using CuInS2 (CIS) and ZnCuInSe (ZCISe) quantum dots (QDs), as well as Mn-doped CdS (Mn-CdS) nanoparticles (NPs) as sensitizers. The use of CIS and ZCISe QDs and Mn-CdS NPs extends the light harvest region to visible light. The photoelectric conversion efficiency was consequently improved, with a photocurrent density of 12.5 mA cm−2, about 60 times that of pure TiO2 nanowires. The germicidal efficiency of the photocatalyst was assessed by passivation of E. coli, 96% bacteria in 50 ml 105 colony forming units (CFU) ml−1 solution were killed within 50 min. Besides the high efficiency, the composite has good stability and satisfactory recycling performance. The antibiotic mechanism was also performed by using photoluminescence and a scavenging agent of different active matter, revealing that the photo-generated holes play a major role in the sterilization process.
研究领域:
微生物学
·
材料科学
·
环境科学
✓ 已验证
DOI
高影响因子
iScience
·
2023
·
IF 5.8
Sirui Tan,Shaila Afroj,Daiqi Li,Md Rashedul Islam,Jihong Wu,Guangming Cai,Nazmul Karim,Zhong Zhao
Summary Electroconductive textile yarns are of particular interest for their use as flexible and wearable sensors without compromising the properties and comfort of usual textiles. However, the detection of fine actions of the human body is quite challenging since it requires both the relatively higher sensitivity and stability of the sensor. Herein, highly sensitive, ultra-stable, and extremely durable piezoresistive wearable sensors were prepared by loading N-doped rGO and polydopamine-coated carbon nanotubes into silicon rubber tube. The wearable sensors thus produced show an excellent ability to sense subtle movement or stimuli with good sensitivity and repeatability. Furthermore, by bending the straight conductive silicon rubber tube (CSRT) into three different patterns, its sensitivity was then dramatically increased. Finally, the CSRT was found capable of sensing cardiorespiratory signals, indicating that the sensor would be an important step toward realizing bio-signal sensing for next-generation personalized health care applications.
研究领域:
材料科学
·
生物医学工程
·
可穿戴技术
·
医疗保健
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）