MOLECULES
·
2021
·Vol. 26
·期 15
·4472
·
IF 4.412
Rurui Li,Yuerong Ru,Zhenxing Wang,Xiahong He,Kin-Weng Kong,Tingting Zheng,Xuechun Zhang
In this study, we aimed to investigate the chemical components and biological activities ofMusella lasiocarpa, a special flower that is edible and has functional properties. The crude methanol extract and its four fractions (petroleum ether, ethyl acetate, n-butanol, and aqueous fractions) were tested for their total antioxidant capacity, followed by their α-glucosidase, acetylcholinesterase, and xanthine oxidase inhibitory activities. Among the samples, the highest total phenolic and total flavonoid contents were found in the ethyl acetate (EtOAc) fraction (224.99 mg GAE/g DE) and crude methanol extract (187.81 mg QE/g DE), respectively. The EtOAc fraction ofMusella lasiocarpaexhibited the strongest DPPH· scavenging ability, ABTS·+scavenging ability, and α-glucosidase inhibitory activity with the IC50values of 22.17, 12.10, and 125.66 μg/mL, respectively. The EtOAc fraction also showed the strongest ferric reducing antioxidant power (1513.89 mg FeSO4/g DE) and oxygen radical absorbance capacity ability (524.11 mg Trolox/g DE), which were higher than those of the control BHT. In contrast, the aqueous fraction demonstrated the highest acetylcholinesterase inhibitory activity (IC50= 10.11 μg/mL), and the best xanthine oxidase inhibitory ability (IC50= 5.23 μg/mL) was observed from the crude methanol extract as compared with allopurinol (24.85 μg/mL). The HPLC-MS/MS and GC-MS analyses further revealed an impressive arsenal of compounds, including phenolic acids, fatty acids, esters, terpenoids, and flavonoids, in the most biologically active EtOAc fraction. Taken together, this is the first report indicating the potential ofMusella lasiocarpaas an excellent natural source of antioxidants with possible therapeutic, nutraceutical, and functional food applications.Keywords:Musella lasiocarpa;antioxidant activity;α-glucosidase;xanthine oxidase;acetylcholinesterase;HPLC-MS/MS;GC-MS
研究领域:
植物化学
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药理学
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营养学
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PubMed
高影响因子
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2021
·Vol. 247
·期 10
·2591-2608
·
IF 2.998
Wu Yi,Li LinZhu,Yuan WeiQiong,Hu JianZhong,Lv ZhaoLin
Tree peony essential oil (EO) is a critical material for flavor and function in the industrial fields of perfume, medicine and food. To conduct an overall analysis of different varieties of tree peony EOs, the composition, aroma characteristics and antioxidant activity of ZiBan and FengDan EOs were analyzed by two-dimensional gas chromatography and time-of-flight mass spectrometry (GC × GC-TOF/MS) and the odor activity value (OAV). A total of 151 and 123 compounds were identified in ZiBan and FengDan EOs, respectively, in which ZiBan EO was characterized by alcohol, alkane and acid, and FengDan EO was represented by aldehyde, alcohol and terpene. Nevertheless, there were great differences in the content of coexisting substances in the two kinds of EOs. The aroma result indicated that the characteristic aroma and intensity of the two kinds of tree peony EOs were completely different; the aroma of ZiBan EO was mainly composed of fruity and floral aromas, whereas that of FengDan EO primarily consisted of fruity, fatty, grass and floral odors. The antioxidant activities of ZiBan EO was better than that of FengDan EO. The total antioxidant activities of ZiBan and FengDan EOs were found to be 0.020 mg Vc/mL EO and 0.003 mg Vc/mL EO, while the DPPH radical scavenging abilities of ZiBan and FengDan EOs were 24.77% and 21.60%, respectively.
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FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Yun-Jiao Ma,Ping Li,Bei-Wei Zhu,Ming Du,Xian-Bing Xu
The comprehensive determination of fatty acids without derivatization, including short-chain fatty acids (SCFAs), medium-chain fatty acids (MCFAs) and long-chain fatty acids (LCFAs), is a big challenge but powerful for lipidomics in biology, food, and environment. Herein, the dual mode unity solid-phase microextraction (DMU-SPME) combined with gas chromatography-flame ionization detector (GC-FID) or mass spectrometry (MS) was proposed as a powerful method for the determination of comprehensive free fatty acids in real samples. Under the optimized DMU-SPME conditions, the proposed method has good linearity (R 2 ≥ 0.994) and low limits of determination (0.01–0.14 mg/L). In the stability analysis, the intra-day relative standard deviation was 1.39–12.43 %, and the inter-day relative standard deviation was 2.84–10.79 %. The recoveries of selected 10 fatty acids in real samples ranged from 90.18 % to 110.75 %, indicating that the method has good accuracy. Fatty acids ranging from C2 to C22 were detected in real samples by the untargeted determination method of DMU-SPME combined with gas chromatography-mass spectrometry (GC–MS). The DMU-SPME method proposed in this study can be used for lipid metabolism analysis and free fatty acid determination in the fields of biology, food, and environment.
研究领域:
生物化学
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食品科学
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环境科学
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PubMed
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Food Chemistry-X
·
2024
·
IF 6.5
Jianfeng Liang,Hailin Wu,Mingfei Lu,Ya Li
This study used headspace solid-phase microextraction-gas chromatography–mass spectrometry and multivariate statistical analysis to comprehensively analyze the volatile components in Liupao tea samples throughout fermentation. In total, 1009 volatile organic compounds were detected and identified, including terpenoids, heterocyclic compounds, esters, ketones, hydrocarbons, alcohols, aromatics, and acids. Principal component and hierarchical cluster analyses, characterize the volatile components of Liupao tea samples were characterized at various fermentation stages. Orthogonal partial least squares discriminant analysis identified 248 differentiating compounds (VIP ≥ 1, P < 0.05, and |Log 2 FC| ≥ 1.0) during fermentation. K-means clustering analysis showed that 11 metabolites increased significantly throughout the fermentation process, whereas 31 metabolites decreased continuously. Annotation of these differential compounds revealed significant changes in sensory flavor characteristics in “green, sweet, fruity, floral, and woody” flavors. The results demonstrated significant variations in the volatile components of Liupao tea fermentation, along with notable changes in flavor characteristics.
研究领域:
食品科学
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分析化学
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植物代谢
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DOI
PubMed
高影响因子
Cell Reports
·
2026
·Vol. 45
·期 10
·
IF 7.7
Juan Zhang,Mengjiao Lan,Hui Fu,Xucong Teng,Erwei Zhang,Xiran Peng,Chen Li,Bingjie Pan,Hang Yu,Yu Cai,Yan Yan,Yulan Jin,Zongyi Bo,Pumin Zhang,Ying Liu,Dongli Pan,Wei Wu,Jiyong Zhou
Many α-herpesviruses, such as herpes simplex virus type 1 (HSV-1) and pseudorabies virus, possess GC-rich genomes prone to forming G-quadruplex (G4) structures. However, the impact of these G4 structures on viral genome maintenance is unknown. Here, we demonstrate that G4s impede lagging strand DNA synthesis in the viral genome, generating single-stranded DNA (ssDNA) gaps that activate an ATR-mediated replication checkpoint. To complete genome duplication, these viruses co-opt host ssDNA gap prevention pathways, including PARP-mediated backup Okazaki fragment maturation and post-replicative gap-filling mechanisms, to resolve G4-induced ssDNA gaps. Pharmacological G4 stabilization, combined with inhibition of these pathways, synergistically inhibits viral replication. In terminally differentiated neurons, only gap-filling pathways are essential for virus replication, and disrupting them or stabilizing G4s robustly impairs HSV-1 reactivation in trigeminal ganglion explants. Collectively, our findings identify G4-induced ssDNA gaps as key barriers to α-herpesvirus replication and reactivation and highlight host ssDNA gap prevention pathways as promising therapeutic targets.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2026
·
IF 4.1
Chen Hongze,Wan Yuebing,Li Kangcong,Li Xiuqin,Li Xiaomin,Chu Hongtao,Zhang Qinghe
Organophosphorus flame retardants (OPFRs) are widespread environmental contaminants with bioaccumulative potential and associated human health risks. The lack of commercial reference standards for many OPFRs limits the accuracy of their quantification. In this study, a compound-independent calibration (CIC) method using gas chromatography-inductively coupled plasma mass spectrometry (GC-ICP-MS) was developed for the simultaneous quantification of seven alkyl OPFRs, with tripropyl phosphate (TnPP) as a single universal calibrant. Key parameters were optimized: a DB-5MS column (15 m × 0.25 mm × 0.10 μm) with pulsed splitless injection (70 psi, 280 °C), and ICP-MS settings including an RF power of 1600 W, sampling depth of 7.5 mm, dilution gas flow rate of 0.5 mL/min, and octopole voltage of − 5 V. The method exhibited excellent linearity ( R 2 = 0.9994 to 0.9999) over the range of 0.01–1.0 μg/mL. Limits of detection and quantification were 0.22 to 0.91 ng/mL and 0.75 to 3.02 ng/mL, respectively. For six of the seven OPFRs (excluding TEHP), the relative P response ratios compared to TnPP ranged from 0.91 to 1.01, confirming effective structure-independent calibration. The matrix effect in rice was moderate (enhancement factors 1.13–1.35). Quantitative results obtained by the CIC method agreed well with those from an external standard approach, with relative deviations within −8.54% to +7.78%. This work demonstrates that CIC-GC-ICP-MS using a single, readily available calibrant provides a reliable, practical solution for quantifying alkyl OPFRs in complex food matrices, effectively overcoming the challenge posed by the scarcity of compound-specific reference standards. Graphical
The alternative text for this image may have been generated using AI.
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高影响因子
CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Ye Li,Jia-Ning Gao,Jun Liang,Xin-Hua Zhu,Hai-Xue Kuang,Yong-Gang Xia
The accurate characterization of glycosyl linkages in complex carbohydrates is always a challenging task due to the occurrence of isomer diversity and the unavailability of known standards. Because of the significant difference between head and tail in structures, this article built a complete methylation analysis library based on partially methylated aldononitrile acetates (PMANs) for facilitated identification of complex glycosidic linkages in polysaccharides. This library covered full EI-MS spectra and detailed fragmentation behaviors of corresponding PMANs ascribed to 46 aldohexoses, 32 aldopentoses and 16 deoxyhexoses. Furthermore, the elute behaviors of these PMANs are summarized on two capillary columns ascribed to complementary polarities. It is quite benefit to elucidate the glycosyl linkages of polysaccharides for inexperienced researchers in this field. The newly established PMAN library has been conformed to be an effective and alternative strategy for glycosyl linkage analysis of polysaccharides.
研究领域:
分析化学
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糖生物学
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PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Wanxiang Yu,Ye Liu,FuBao Qiu,Yuyan Chen,Yingsi Huang,Xiaoming Luo
Dehydroacetic acid (DHA) is a commonly used preservative widely applied in food products. Currently, the quantitative analysis process for DHA remains cumbersome, involving multiple toxic organic reagents. To address these issues, this study leveraged the low-toxicity, environmentally friendly, and rapid characteristics of aqueous two-phase systems (ATPS) to develop a pretreatment method suitable for the extraction and purification of DHA. This method is compatible with both high-performance liquid chromatography (HPLC) and gas chromatography (GC) techniques. For HPLC, acidic chromatographic conditions were developed to mitigate issues such as peak tailing and retention time drift, improving peak shapes and stabilizing retention times. For GC, appropriate chromatographic conditions were established for accurate quantitative analysis of DHA. The study focused on screening appropriate ATPS types and optimizing the composition ratios of their constituent components to achieve optimal extraction and purification efficiency for DHA. Method validation and sample analysis were conducted for both ATPS-HPLC and ATPS-GC approaches. Under the ATPS conditions composed of 5.00 mL water, 2.00 mL n-propanol, and 7.00 g sodium dihydrogen phosphate, results demonstrated that the ATPS-HPLC (GC) method is accurate, reliable, rapid, and environmentally friendly, with detection limits of 2.00 × 10 −4 g/kg (6.00 × 10 −4 g/kg), average recoveries of 93.7–104 % (98.3–107 %), and relative standard deviations (RSDs) of 0.7–5.2 % (1.9–6.8 %).
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Biomed Research International
·
2018
·
IF 2.583
Xuemei Ye,Aifang Huang,Xianqin Wang,Congcong Wen,Lufeng Hu,Guanyang Lin
Linezolid has been widely used in serious infections for its effective inhibiting effect against multidrug-resistant gram-positive pathogens. However, linezolid caused severe adverse reactions, such as thrombocytopenia, anaemia, optic neuropathy, and near-fatal serotonin syndrome. In order to investigate the toxicity of linezolid, twenty-four Sprague-Dawley rats were randomly divided into: control group (n=7), low-group (n=8), and high-group (n=9). The rats of low-group and high-group were given by gavage with linezolid 60 and 120 mg/kg/day for 7 days, respectively. The serum concentration of linezolid was determined by high performance liquid chromatography (HPLC); blood metabolic change was analyzed by gas chromatography-mass spectrometer (GC-MS). Adenosine triphosphate (ATP) concentration in HepG2-C3A after being cultured with linezolid was determined by HPLC. The results showed that there were six metabolites and nine metabolites had statistical differences in low-group and high-group (P<0.05). The trimethyl phosphate was the most significant indicator in those changed metabolites. Except for d-glucose which was slightly increased in low-group, octadecanoic acid, cholest-5-ene, hexadecanoic acid, α-linolenic acid, eicosapentaenoic acid, 9,12-Octadecadienoic acid, and docosahexaenoic acid were all decreased in low-group and high-group. ATP concentration was decreased in HepG2-C3A after cultured with linezolid. In conclusion, the toxicity of linezolid is related to its serum concentration. Linezolid may inhibit the synthesis of ATP and fatty acid.
研究领域:
药理学
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毒理学
·
代谢研究
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PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Yang-bo Xiao,Rongji Tian,Ying-ying Shen,Han Tang,Jian-an Huang,Wenliang Wu,Shu-guang Zhang,Xia Yin
Leaf grade is a critical factor influencing black tea aroma formation, yet the precise relationship between leaf grade and aroma characteristics, along with their key volatile compounds, remains unclear. This study employed sensory evaluation combined with headspace solid phase microextraction/gas chromatography-Mass spectrometry (HS-SPME/GC-MS), odor activity values (OAV), partial least squares (PLS) regression, and correlation analysis to investigate the association between leaf grades and aroma attributes, as well as their key volatile compounds. Sensory evaluation and quantitative analysis revealed that as fresh tea leaf grade decreased, the sweet aroma attribute gradually declined, while the floral aroma initially decreased before increasing. GC-MS and OAV analyses identified 22 and 23 differential aroma-active compounds in ‘Jinguanyin’ (JGY) and ‘Jinmudan’ (JMD) black teas of different grades, respectively. Furthermore, PLS modeling and correlation analysis determined that β-damascenone, β-myrcene, geraniol, linalool, and trans -β-ocimene were key compounds responsible for sweet aroma variations across leaf grades, whereas methyl jasmonate, β-ionone, and jasmine lactone were identified as critical compounds influencing floral aroma differences. This study deciphered the chemical basis of how leaf grades affect black tea aroma quality, providing theoretical guidance for precision processing of black tea.
研究领域:
食品科学
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分析化学
·
感官分析
·
植物生物化学
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高影响因子
ChemistrySelect
·
2025
·Vol. 10
·期 27
·e01763
·
IF 2
Junjie Tang,Shiyu Peng,Guizhi Tang,Yuxue Mo,Chen Wang
Graphical This paper presents a urchin-like graphitized carbon-coated SnO₂ (GC-SnO₂) composite. Its spine-like carbon structure with branched networks alleviates stress concentration in SnO₂ anodes during cycling, enabling efficient ion transport. This design enhances high-rate performance and structural stability of lithium-ion batteries. Tin dioxide (SnO₂) has emerged as a promising anode for lithium-ion batteries (LIBs) due to its high theoretical capacity and natural abundance. However, it suffers from severe volume expansion during lithiation/delithiation processes and limited rate capability. To address these challenges, this work rationally designed an urchin-like-graphitized carbon (GC)-SnO₂ microsphere composite (denoted for GC-SnO 2 ) through hydrothermal synthesis followed by controlled annealing. The hierarchical architecture consists of hollow SnO 2 nanospheres encapsulated within branched graphitized carbon protrusions, forming a hierarchically porous network. This structural design enables three key advantages: (1) accelerated Li + diffusion through interconnected channels, (2) stress buffering via spiny carbon branches to mitigate SnO 2 pulverization, and (3) enhanced conductivity through conformal GC coating. Benefiting from these synergistic features, the GC-SnO 2 anode exhibits outstanding cyclability, maintaining 765 mA h g −1 after 100 cycles at 0.2 A g −1 . Notably, it achieves 538 mA h g −1 at 2 A g −1 over 1000 cycles, surpassing bare SnO 2 (89 mA h g −1 ) and exceeding the performance of most reported SnO 2 -based anodes. This work provides an effective strategy for designing stress-tolerant oxide/carbon composites toward high-energy-density LIBs.
研究领域:
材料科学
·
储能
·
纳米技术
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电化学
·
复合材料
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高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Yujiao Cheng,Guijie Li,Houjiu Wu,Guolu Liang,Hua Wang
Commercial mandarin juice often exhibits flavor deterioration during storage. The changes in volatile flavor profiles were investigated in mandarin juice stored under three different temperature conditions (4 °C, 25 °C, and 40 °C). The application of multidimensional gas chromatography-mass spectrometry/olfactometry (MDGC-MS/O) coupled with aroma extract dilution analysis (AEDA) identified 31 odorants in stored mandarin juice, including seven off-flavor contributing compounds: methanethiol (unpleasant, repulsive), dimethyl sulfide (sulfur, cabbage), methional (cooked potato), p -cymene (solventy), 4-vinylguaiacol (medicine), terpinen-4-ol (wood), and α-terpineol (floral, mint). 4-Vinylguaiacol was also identified in mandarin juice for the first time. In addition, 64 volatile compounds determined by GC-MS/pulsed flame photometric detector (GC-MS/PFPD) were classified as alcohols, terpenes, volatile sulfur compounds (VSCs), aldehydes, esters, ketones, and phenols. Volatile compound differences were observed in mandarin juice under different storage conditions by heat-map generation and principal component analysis (PCA). The results revealed that high-temperature storage led to a rapid increase in the concentration of total alcohols and VSCs. Furthermore, the flavor deterioration markers α-terpineol and dimethyl sulfide were identified based on off-flavor contribution and variable importance in projection (VIP) (score >1) values in the PLS-DA model. These two compounds are valuable for marker-assisted flavor deterioration analysis in mandarin juice during storage.
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危险品化学品经营许可证（带存储）